RLG00000003745

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000302
Physical Location & Seq
Forward (+)
52237994 .. 52240024
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003745

Sequence Viewer

Length: 2031 bp
ATGGTTTATGCATACCACAATGTGGCAACTGGCTTCGCTGCAAAGCTGACAGTAGAGGAAGTTGAAGAGATGGAAAAGAAAGATGGGTTTATCTCCGCTCATCCAGAACGAAATCTGCAATTGCACACAACTCGTAGTCCTAACTTCTTGGGGCTGCAGCAAGGAATGGGACTTGGTAAAGGGCCTAACTATGGTGAAGGTGTGATTATTGGAATTTTGGATACTGGGATATTCCCAGACCATCCTTCATTTAGTGATGAAGGAGTACCACCTCCTCCAGCTAAATGGAAAGGCAAATGTGATTTCAGCGGGACAGTCTGCAATAACAAACTTATCGGTGCCAGAAGTTTTAATGGTGGAAAAACTACTGGAGGCCCTCCAGTTGACGATGAAGGCCATGGAACTCACACGTCAAGCACAGCTGGAGGAAATTTTGTGAAAGGTGCCAGCGTGTTTGGAATGGCCAACGGCACAGCAACTGGCGTGGCACCTCATGCTCACTTGGCAATGTACAAAGTATGCACAGACGAGGGTTGTTCTGAATCAGATATTATAGCTGCTATGGACACTGCTGTTGATGATGGCGTGGATGTGCTATCCCTTTCACTCGGTGGTGGCTCAGCTGATTTTTATGCTGATGGAATTGCAGTCAGCGCATTTGGAGCAATTCAAAAGGGAATCTTTGTCAGCTGTTCAGCTGGAAATGCTGGCCCTGACAATATGACTTTATCCAATGAAGCTCCATGGATGCTCACGGTTGGAGCAAGCACCATTGATAGAAACATAACAGCAACAGCAAAGCTTGGAAATGGGCAAGAATATGACGGGGAATCACTATTCCAGCCTAAAGATTTTGGTTCAAAACTGTTGCCTCTTGTTTATGCAGGTGCACATAGCAATGATTCATCAGCTTTGTGTGACGAAGGATCTCTTGAAAATGTTGAAGGGAAAGTAGTGGTCTGTGAGGTAGGTGGAGGAATTGGAAGAATTGCACAAGGGGTAGAAGTGAAAAGAGCTGGTGGTGTTGCCATGATTCTTGTCAACCAAGACTTTGCTGGCTATACCACCATAGCAGACGCTCACGTGCTTCCGGCAACACATGTGAGTTATGCTGCAGGGGTGAGTATCAAGAGCTATATAAACTCAACCTCAACTCCTACAGCTACAATCTTATTCAAAGGCACTGTCATCGGTGATCAGCTTGCTCCCAAGGTTGCTTTCTTTTCATCAAGAGGACCAAACCGGGCAAGCCCTGCAATTTTGAAACCTGACATTATTGGTCCCGGTGTGAGCATTCTAGCAGCCTGGCCTTTTTCGGTGGATAATACCACAGAATCCAAGGCCACATTTAACATTATTTCAGGTACTTCAATGTCATGCCCTCACCTAAGTGGCATTGCAGCCTTGCTCAAGAGCTCACACCCTGACTGGTCACCAGCTGCTATTAAGTCTGCAATGATGACAACTGCTGATGTCCTAAACCTCGCTGGCTCGGCCATTCTTGATCAAACACTTCTTGCAGCAGACCTCTTTGCCATCGGCGCAGGCCATGTTAACCCTTCAAAAGCAAATGACCCTGGGCTCATCTACGACATACAGCCAGAGGATTACATTCCTTACTTGTGTGGTTTGAATTACACAAGTGAACAGACAGCGGCAATCACCCAACAAAAAGTGAATTGCTCTAAAGTAGGAGTCATACCAGAAGCACAGCTAAACTATCCCACATTTTGTATCTATATAGAGTCTGGTGGTGAGCCTCAGAATTACACAAGAACCTTGACCAATGTTGGCCCAGCTAATTCAACTTACAAATTGGCTCCCCTGAGACTACATAAAATGGACATCACTGTGCTACCTGAGGTGCTTACATTTACAGAGATCAACCAGAAATTGACATACCGTGTGGTGTTTTCCGCACAAGACGGAGCTGGGAAAGATGGTATACCATTTTCTCAGGGGTATTTGAGTTGGGTGTCTCATCAGCATTCTGTTAACAGCCAAATATCTGTGGTCTTTGACTATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

677

Amino Acids

70.78

Weight (kDa)

5.12

Isoelectric Point (pI)

30.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 65 - 492 4.9e-48 Subtilase family
PA PF02225 291 - 375 1.3e-10 PA domain
fn3_6 PF17766 571 - 671 2.6e-21 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 875
Acc36I ACCTGC 1 cut(s) 875
AccB1I GGYRCC 3 cut(s) 338, 443, 487
AccB7I CCANNNNNTGG 1 cut(s) 22
AccBSI CCGCTC 1 cut(s) 98
AccI GTMKAC 1 cut(s) 1945
AciI CCGC 4 cut(s) 96, 309, 1655, 1917
AclWI GGATC 1 cut(s) 934
AcoI YGGCCR 2 cut(s) 462, 1494
AcsI RAATTY 2 cut(s) 213, 430
AcvI CACGTG 1 cut(s) 1084
AdeI CACNNNGTG 2 cut(s) 22, 611
AfaI GTAC 3 cut(s) 267, 512, 1366
AfiI CCNNNNNNNGG 2 cut(s) 22, 191
AflIII ACRYGT 2 cut(s) 408, 1099
AjiI CACGTC 1 cut(s) 411
AjnI CCWGG 2 cut(s) 1304, 1576
AjuI GAANNNNNNNTTGG 2 cut(s) 1202, 1234
AleI CACNNNNGTG 1 cut(s) 1389
Alw21I GWGCWC 2 cut(s) 892, 1418
Alw26I GTCTC 2 cut(s) 1822, 1983
Alw44I GTGCAC 1 cut(s) 888
AlwI GGATC 1 cut(s) 934
AlwNI CAGNNNCTG 1 cut(s) 479
ApaLI GTGCAC 1 cut(s) 888
ApeKI GCWGC 9 cut(s) 38, 154, 157, 557, 1112, 1301, 1400, 1439, 1520
ApoI RAATTY 2 cut(s) 213, 430
ArsI GACNNNNNNTTYG 2 cut(s) 1170, 1202
AspLEI GCGC 2 cut(s) 656, 1544
AspS9I GGNCC 6 cut(s) 182, 374, 710, 1235, 1280, 1793
AsuC2I CCSGG 2 cut(s) 1244, 1284
AsuHPI GGTGA 7 cut(s) 206, 1132, 1205, 1376, 1425, 1654, 1766
AvaII GGWCC 2 cut(s) 1235, 1280
AxyI CCTNAGG 1 cut(s) 1860
BaeGI GKGCMC 1 cut(s) 892
BalI TGGCCA 1 cut(s) 464
BanI GGYRCC 3 cut(s) 338, 443, 487
BanII GRGCYC 2 cut(s) 1418, 1584
BbrPI CACGTG 1 cut(s) 1084
Bbv12I GWGCWC 2 cut(s) 892, 1418
BbvI GCAGC 9 cut(s) 25, 141, 169, 544, 1099, 1313, 1412, 1426, 1532
BccI CCATC 7 cut(s) 64, 77, 249, 575, 632, 1544, 1934
BceAI ACGGC 1 cut(s) 484
BcgI CGANNNNNNTGC 4 cut(s) 113, 147, 1171, 1205
BciT130I CCWGG 2 cut(s) 1306, 1578
BciVI GTATCC 1 cut(s) 214
BclI TGATCA 2 cut(s) 1195, 1504
BcnI CCSGG 2 cut(s) 1244, 1284
BcoDI GTCTC 2 cut(s) 1822, 1983
BfaI CTAG 1 cut(s) 1298
BfmI CTRYAG 3 cut(s) 155, 1113, 1158
BfuAI ACCTGC 1 cut(s) 875
BfuI GTATCC 1 cut(s) 214
BlpI GCTNAGC 1 cut(s) 619
Bme1390I CCNGG 4 cut(s) 1244, 1284, 1306, 1578
Bme18I GGWCC 2 cut(s) 1235, 1280
BmgBI CACGTC 1 cut(s) 411
BmgT120I GGNCC 6 cut(s) 182, 374, 710, 1235, 1280, 1793
BmiI GGNNCC 5 cut(s) 340, 445, 489, 1282, 1821
BmrFI CCNGG 4 cut(s) 1244, 1284, 1306, 1578
BmrI ACTGGG 1 cut(s) 234
BmsI GCATC 1 cut(s) 738
BmuI ACTGGG 1 cut(s) 234
BpmI CTGGAG 4 cut(s) 261, 363, 390, 444
Bpu1102I GCTNAGC 1 cut(s) 619
BpuEI CTTGAG 1 cut(s) 1394
BpuMI CCSGG 2 cut(s) 1244, 1284
BsaAI YACGTR 1 cut(s) 1084
BsaJI CCNNGG 6 cut(s) 397, 743, 1209, 1338, 1576, 1577
BsaXI ACNNNNNCTCC 2 cut(s) 1138, 1168
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 191
Bse1I ACTGG 6 cut(s) 34, 229, 373, 380, 484, 1433
Bse21I CCTNAGG 1 cut(s) 1860
Bse3DI GCAATG 4 cut(s) 513, 904, 1395, 1461
BseBI CCWGG 2 cut(s) 1306, 1578
BseDI CCNNGG 6 cut(s) 397, 743, 1209, 1338, 1576, 1577
BseGI GGATG 4 cut(s) 100, 241, 595, 753
BseLI CCNNNNNNNGG 2 cut(s) 22, 191
BseMI GCAATG 4 cut(s) 513, 904, 1395, 1461
BseMII CTCAG 5 cut(s) 633, 1775, 1817, 1851, 1970
BseNI ACTGG 6 cut(s) 34, 229, 373, 380, 484, 1433
BseRI GAGGAG 1 cut(s) 264
BseSI GKGCMC 1 cut(s) 892
BseXI GCAGC 9 cut(s) 25, 141, 169, 544, 1099, 1313, 1412, 1426, 1532
BseYI CCCAGC 2 cut(s) 1795, 1931
BshNI GGYRCC 3 cut(s) 338, 443, 487
BsiHKAI GWGCWC 2 cut(s) 892, 1418
BsiSI CCGG 3 cut(s) 1091, 1243, 1284
BslFI GGGAC 3 cut(s) 183, 325, 1266
BslI CCNNNNNNNGG 2 cut(s) 22, 191
BsmAI GTCTC 2 cut(s) 1822, 1983
BsmFI GGGAC 3 cut(s) 183, 325, 1266
BsmI GAATGC 2 cut(s) 1293, 1987
Bsp1286I GDGCHC 3 cut(s) 892, 1418, 1584
Bsp1407I TGTACA 1 cut(s) 510
Bsp143I GATC 4 cut(s) 926, 1195, 1504, 1881
Bsp1720I GCTNAGC 1 cut(s) 619
Bsp19I CCATGG 2 cut(s) 397, 743
BspACI CCGC 4 cut(s) 96, 309, 1655, 1917
BspCNI CTCAG 5 cut(s) 632, 1774, 1818, 1852, 1969
BspLI GGNNCC 5 cut(s) 340, 445, 489, 1282, 1821
BspMAI CTGCAG 2 cut(s) 159, 1117
BspMI ACCTGC 1 cut(s) 875
BspPI GGATC 1 cut(s) 934
BspT107I GGYRCC 3 cut(s) 338, 443, 487
BsrBI CCGCTC 1 cut(s) 98
BsrDI GCAATG 4 cut(s) 513, 904, 1395, 1461
BsrGI TGTACA 1 cut(s) 510
BsrI ACTGG 6 cut(s) 34, 229, 373, 380, 484, 1433
BssECI CCNNGG 6 cut(s) 397, 743, 1209, 1338, 1576, 1577
BssMI GATC 4 cut(s) 926, 1195, 1504, 1881
BssNAI GTATAC 1 cut(s) 1946
BssT1I CCWWGG 4 cut(s) 397, 743, 1209, 1338
Bst1107I GTATAC 1 cut(s) 1946
Bst2UI CCWGG 2 cut(s) 1306, 1578
Bst4CI ACNGT 7 cut(s) 52, 316, 757, 867, 1186, 1852, 1904
Bst6I CTCTTC 1 cut(s) 60
BstAPI GCANNNNNTGC 2 cut(s) 494, 1253
BstAUI TGTACA 1 cut(s) 510
BstBAI YACGTR 1 cut(s) 1084
BstC8I GCNNGC 8 cut(s) 448, 709, 766, 1057, 1203, 1249, 1489, 1546
BstDEI CTNAG 6 cut(s) 619, 1388, 1761, 1826, 1860, 1956
BstDSI CCRYGG 2 cut(s) 397, 743
BstEII GGTNACC 1 cut(s) 1431
BstF5I GGATG 4 cut(s) 100, 241, 595, 753
BstHHI GCGC 2 cut(s) 656, 1544
BstKTI GATC 4 cut(s) 929, 1198, 1507, 1884
BstMAI GTCTC 2 cut(s) 1822, 1983
BstMBI GATC 4 cut(s) 926, 1195, 1504, 1881
BstMWI GCNNNNNNNGC 8 cut(s) 494, 503, 653, 662, 704, 1253, 1493, 1541
BstNI CCWGG 2 cut(s) 1306, 1578
BstNSI RCATGY 1 cut(s) 1103
BstPI GGTNACC 1 cut(s) 1431
BstSCI CCNGG 4 cut(s) 1242, 1282, 1304, 1576
BstSFI CTRYAG 3 cut(s) 155, 1113, 1158
BstSLI GKGCMC 1 cut(s) 892
BstV1I GCAGC 9 cut(s) 25, 141, 169, 544, 1099, 1313, 1412, 1426, 1532
BstX2I RGATCY 1 cut(s) 926
BstXI CCANNNNNNTGG 1 cut(s) 285
BstYI RGATCY 1 cut(s) 926
BstZ17I GTATAC 1 cut(s) 1946
Bsu36I CCTNAGG 1 cut(s) 1860
BsuI GTATCC 1 cut(s) 214
BtgI CCRYGG 2 cut(s) 397, 743
BtrI CACGTC 1 cut(s) 411
BtsCI GGATG 4 cut(s) 100, 241, 595, 753
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 3 cut(s) 567, 1182, 1848
BveI ACCTGC 1 cut(s) 875
Cac8I GCNNGC 8 cut(s) 448, 709, 766, 1057, 1203, 1249, 1489, 1546
CaiI CAGNNNCTG 1 cut(s) 479
CfoI GCGC 2 cut(s) 656, 1544
Cfr13I GGNCC 6 cut(s) 182, 374, 710, 1235, 1280, 1793
CseI GACGC 1 cut(s) 1085
Csp6I GTAC 3 cut(s) 266, 511, 1365
CspCI CAANNNNNGTGG 2 cut(s) 465, 500
CviAII CATG 7 cut(s) 398, 494, 744, 1030, 1100, 1377, 1550
CviQI GTAC 3 cut(s) 266, 511, 1365
DdeI CTNAG 6 cut(s) 619, 1388, 1761, 1826, 1860, 1956
DpnI GATC 4 cut(s) 928, 1197, 1506, 1883
DpnII GATC 4 cut(s) 926, 1195, 1504, 1881
DraIII CACNNNGTG 2 cut(s) 22, 611
EaeI YGGCCR 2 cut(s) 462, 1494
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
Ecl136II GAGCTC 1 cut(s) 1416
Eco130I CCWWGG 4 cut(s) 397, 743, 1209, 1338
Eco24I GRGCYC 2 cut(s) 1418, 1584
Eco47I GGWCC 2 cut(s) 1235, 1280
Eco53kI GAGCTC 1 cut(s) 1416
Eco72I CACGTG 1 cut(s) 1084
Eco81I CCTNAGG 1 cut(s) 1860
Eco91I GGTNACC 1 cut(s) 1431
EcoICRI GAGCTC 1 cut(s) 1416
EcoO109I RGGNCCY 2 cut(s) 182, 374
EcoO65I GGTNACC 1 cut(s) 1431
EcoRII CCWGG 2 cut(s) 1304, 1576
EcoT14I CCWWGG 4 cut(s) 397, 743, 1209, 1338
EcoT22I ATGCAT 1 cut(s) 13
EcoT38I GRGCYC 2 cut(s) 1418, 1584
ErhI CCWWGG 4 cut(s) 397, 743, 1209, 1338
FaeI CATG 7 cut(s) 401, 497, 747, 1033, 1103, 1380, 1553
FalI AAGNNNNNCTT 4 cut(s) 665, 697, 915, 947
FaqI GGGAC 3 cut(s) 183, 325, 1266
FatI CATG 7 cut(s) 397, 493, 743, 1029, 1099, 1376, 1549
FauI CCCGC 1 cut(s) 302
FbaI TGATCA 2 cut(s) 1195, 1504
FblI GTMKAC 1 cut(s) 1945
FokI GGATG 4 cut(s) 87, 228, 602, 760
FriOI GRGCYC 2 cut(s) 1418, 1584
FspBI CTAG 1 cut(s) 1298
GlaI GCGC 2 cut(s) 655, 1543
GsaI CCCAGC 2 cut(s) 1799, 1935
GsuI CTGGAG 4 cut(s) 261, 363, 390, 444
HapII CCGG 3 cut(s) 1091, 1243, 1284
HgaI GACGC 1 cut(s) 1085
HhaI GCGC 2 cut(s) 656, 1544
Hin1II CATG 7 cut(s) 401, 497, 747, 1033, 1103, 1380, 1553
Hin6I GCGC 2 cut(s) 654, 1542
HinP1I GCGC 2 cut(s) 654, 1542
HincII GTYRAC 4 cut(s) 385, 1042, 1555, 1996
HindII GTYRAC 4 cut(s) 385, 1042, 1555, 1996
HindIII AAGCTT 1 cut(s) 800
HinfI GANTC 8 cut(s) 542, 678, 830, 902, 1033, 1334, 1695, 1745
HpaI GTTAAC 2 cut(s) 1555, 1996
HpaII CCGG 3 cut(s) 1091, 1243, 1284
HphI GGTGA 7 cut(s) 206, 1132, 1205, 1376, 1425, 1654, 1766
Hpy166II GTNNAC 7 cut(s) 385, 890, 1042, 1555, 1646, 1946, 1996
Hpy188I TCNGA 3 cut(s) 541, 547, 1764
Hpy188III TCNNGA 6 cut(s) 104, 932, 1129, 1230, 1411, 1502
Hpy8I GTNNAC 7 cut(s) 385, 890, 1042, 1555, 1646, 1946, 1996
HpyAV CCTTC 7 cut(s) 191, 254, 255, 386, 917, 938, 1569
HpyCH4III ACNGT 7 cut(s) 52, 316, 757, 867, 1186, 1852, 1904
HpyCH4IV ACGT 2 cut(s) 410, 1083
HpyF10VI GCNNNNNNNGC 8 cut(s) 494, 503, 653, 662, 704, 1253, 1493, 1541
HpyF3I CTNAG 6 cut(s) 619, 1388, 1761, 1826, 1860, 1956
HpySE526I ACGT 2 cut(s) 410, 1083
Hsp92II CATG 7 cut(s) 401, 497, 747, 1033, 1103, 1380, 1553
HspAI GCGC 2 cut(s) 654, 1542
Ksp22I TGATCA 2 cut(s) 1195, 1504
KspAI GTTAAC 2 cut(s) 1555, 1996
Kzo9I GATC 4 cut(s) 926, 1195, 1504, 1881
LmnI GCTCC 6 cut(s) 662, 745, 761, 1210, 1825, 1928
Lsp1109I GCAGC 9 cut(s) 25, 141, 169, 544, 1099, 1313, 1412, 1426, 1532
LweI GCATC 1 cut(s) 738
MaeI CTAG 1 cut(s) 1298
MaeII ACGT 2 cut(s) 410, 1083
MaeIII GTNAC 2 cut(s) 917, 1431
MalI GATC 4 cut(s) 928, 1197, 1506, 1883
MbiI CCGCTC 1 cut(s) 98
MboI GATC 4 cut(s) 926, 1195, 1504, 1881
MboII GAAGA 2 cut(s) 77, 996
MfeI CAATTG 1 cut(s) 119
MflI RGATCY 1 cut(s) 926
MhlI GDGCHC 3 cut(s) 892, 1418, 1584
MlsI TGGCCA 1 cut(s) 464
MluNI TGGCCA 1 cut(s) 464
MlyI GAGTC 2 cut(s) 1704, 1754
MmeI TCCRAC 1 cut(s) 739
Mox20I TGGCCA 1 cut(s) 464
Mph1103I ATGCAT 1 cut(s) 13
MscI TGGCCA 1 cut(s) 464
MseI TTAA 5 cut(s) 351, 1350, 1446, 1554, 1995
MslI CAYNNNNRTG 3 cut(s) 897, 1389, 1850
Msp20I TGGCCA 1 cut(s) 464
MspA1I CMGCKG 7 cut(s) 309, 422, 623, 690, 698, 1439, 1655
MspI CCGG 3 cut(s) 1091, 1243, 1284
MspR9I CCNGG 4 cut(s) 1244, 1284, 1306, 1578
MunI CAATTG 1 cut(s) 119
Mva1269I GAATGC 2 cut(s) 1293, 1987
MvaI CCWGG 2 cut(s) 1306, 1578
MwoI GCNNNNNNNGC 8 cut(s) 494, 503, 653, 662, 704, 1253, 1493, 1541
NciI CCSGG 2 cut(s) 1244, 1284
NcoI CCATGG 2 cut(s) 397, 743
NdeII GATC 4 cut(s) 926, 1195, 1504, 1881
NlaIII CATG 7 cut(s) 401, 497, 747, 1033, 1103, 1380, 1553
NlaIV GGNNCC 5 cut(s) 340, 445, 489, 1282, 1821
NmeAIII GCCGAG 1 cut(s) 1472
NmuCI GTSAC 2 cut(s) 917, 1431
NsiI ATGCAT 1 cut(s) 13
NspI RCATGY 1 cut(s) 1103
OliI CACNNNNGTG 1 cut(s) 1389
PaqCI CACCTGC 1 cut(s) 875
PasI CCCWGGG 1 cut(s) 1577
PciI ACATGT 1 cut(s) 1099
PctI GAATGC 2 cut(s) 1293, 1987
PfeI GAWTC 6 cut(s) 542, 678, 830, 902, 1033, 1334
PflMI CCANNNNNTGG 1 cut(s) 22
PleI GAGTC 2 cut(s) 1703, 1753
PmaCI CACGTG 1 cut(s) 1084
PmlI CACGTG 1 cut(s) 1084
PpsI GAGTC 2 cut(s) 1703, 1753
Ppu21I YACGTR 1 cut(s) 1084
PscI ACATGT 1 cut(s) 1099
Psp124BI GAGCTC 1 cut(s) 1418
Psp6I CCWGG 2 cut(s) 1304, 1576
PspCI CACGTG 1 cut(s) 1084
PspEI GGTNACC 1 cut(s) 1431
PspFI CCCAGC 2 cut(s) 1795, 1931
PspGI CCWGG 2 cut(s) 1304, 1576
PspN4I GGNNCC 5 cut(s) 340, 445, 489, 1282, 1821
PspPI GGNCC 6 cut(s) 182, 374, 710, 1235, 1280, 1793
PstI CTGCAG 2 cut(s) 159, 1117
PstNI CAGNNNCTG 1 cut(s) 479
PsuI RGATCY 1 cut(s) 926
PvuII CAGCTG 5 cut(s) 422, 623, 690, 698, 1439
RsaI GTAC 3 cut(s) 267, 512, 1366
RsaNI GTAC 3 cut(s) 266, 511, 1365
RseI CAYNNNNRTG 3 cut(s) 897, 1389, 1850
SacI GAGCTC 1 cut(s) 1418
SaqAI TTAA 5 cut(s) 351, 1350, 1446, 1554, 1995
Sau3AI GATC 4 cut(s) 926, 1195, 1504, 1881
Sau96I GGNCC 6 cut(s) 182, 374, 710, 1235, 1280, 1793
SchI GAGTC 2 cut(s) 1704, 1754
ScrFI CCNGG 4 cut(s) 1244, 1284, 1306, 1578
SduI GDGCHC 3 cut(s) 892, 1418, 1584
SfaNI GCATC 1 cut(s) 738
SfcI CTRYAG 3 cut(s) 155, 1113, 1158
SinI GGWCC 2 cut(s) 1235, 1280
SmiMI CAYNNNNRTG 3 cut(s) 897, 1389, 1850
SmlI CTYRAG 1 cut(s) 1409
SmoI CTYRAG 1 cut(s) 1409
SsiI CCGC 4 cut(s) 96, 309, 1655, 1917
SspMI CTAG 1 cut(s) 1298
SstI GAGCTC 1 cut(s) 1418
StyD4I CCNGG 4 cut(s) 1242, 1282, 1304, 1576
StyI CCWWGG 4 cut(s) 397, 743, 1209, 1338
TaaI ACNGT 7 cut(s) 52, 316, 757, 867, 1186, 1852, 1904
TaiI ACGT 2 cut(s) 413, 1086
TatI WGTACW 1 cut(s) 510
TauI GCSGC 1 cut(s) 1658
TfiI GAWTC 6 cut(s) 542, 678, 830, 902, 1033, 1334
Tru1I TTAA 5 cut(s) 351, 1350, 1446, 1554, 1995
Tru9I TTAA 5 cut(s) 351, 1350, 1446, 1554, 1995
TscAI CASTG 3 cut(s) 574, 1189, 1855
TseFI GTSAC 2 cut(s) 917, 1431
TseI GCWGC 9 cut(s) 38, 154, 157, 557, 1112, 1301, 1400, 1439, 1520
Tsp45I GTSAC 2 cut(s) 917, 1431
TspDTI ATGAA 6 cut(s) 237, 273, 405, 750, 894, 1215
TspGWI ACGGA 1 cut(s) 1941
TspRI CASTG 3 cut(s) 574, 1189, 1855
Van91I CCANNNNNTGG 1 cut(s) 22
VneI GTGCAC 1 cut(s) 888
VpaK11BI GGWCC 2 cut(s) 1235, 1280
XapI RAATTY 2 cut(s) 213, 430
XceI RCATGY 1 cut(s) 1103
XcmI CCANNNNNNNNNTGG 1 cut(s) 1052
XmiI GTMKAC 1 cut(s) 1945
XspI CTAG 1 cut(s) 1298
Zsp2I ATGCAT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.