Prupe.5G085400_v2.0.a1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000302
Physical Location & Seq
Forward (+)
9741605 .. 9743524
1920 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G085400.1

Sequence Viewer

Length: 1878 bp
ATGGAGACAGAAAAGGGTATGGCATTGCAGGTTATATATCTTGTTGGTTTAACTTTCATGTTTTATTTATCACTCGCAGTTGCACAAGAGGAGCAAAACAACCTGCAAACATATATCGTTTGGGTTGAAAAGCCGGTGTCCCAGAATTCTTTTGCACAATCTCATGAAGATCTGGAGAGTTGGTATCACTCATTTTTGCCTGAAACCAGTGAAAACTCAAACCAGCTGATAAAGAAAAGAATGGTTCATACATACCGTAATGTGGCCATAGGATTTGCAGCAAAACTGACGCCAGAGGAAGTAAAGGCAATGGAAAAGAAAGAAGGGTTTGTGTCAGCTCATCCAGAGAGAATTCTGCCTTTGCAGACTACTCATAGTCCTGACTTCTTGGGTTTACACCAAGGGTTGGGACTTTGGGAACAAACAAACTATGGTGAAGGTGTGATCATTGGAGTTTTGGATACTGGTGTTGCACCATATCATCCTTCATTTAGTGATCAAGGAGTGTCCCCTCCTCCGGCTAAATGGAAAGGCAAATGTGACTTCAATGCGACATTGTGCAATAAAAAACTTATCGGTGCGAAAGATTTCATGGGCTCAAGCAAAGGGAAACCTACAGGGGCTCCATTTGACCAAGATGGCCATGGCACCCACACTTCTAGCACAGCTGCTGGAAATTTTGTGGAAGGCGCAAGTGCATTTGGAGTGGCGAATGGCACAGCTGTTGGCATGGCGCCTTATGCTCACTTGGCCATTTACAGAGTCTGTGGCCTTGATTGTGCTGAAGCTGACATTTTGGCGGCAATGGATGCTGCTGTTGAAGATGGCGTGGATGTGCTCTCCCTCTCACTTGGCGGTCCCTCAATTCCTTTCTATGGGGATGTGATTGCAGTTGGTGCATTTGGAGCAACTCAGAAGGGAATTTTTGTCAGCTGTGCAGCTGGAAACTCTGGTCCTTACCATGCAAGTACCACAGACAGAATATTAAACTTAACCTCAACACCTTCATCCACAATCTTGTTCAATGGCGCCACATCAGATCCACTCGCTCCAAAGGTCGCTACCTTTTCATCAAGAGGACCAAATACTGCAAGCCCTGGAATTCTGAAACCTGATATCATTGGGCCCGGTGTTGACATCCTAGCTGCATGGCCTGTTTCTGTAGACAATGCCACAAAGTCAAATGCAACATTCAACATAATTTCGGGTACCTCAATGTCAACTCCTCACTTAAGTGGCATTGCAGCCTTGCTCAAGAGCTCTCACCCTGACTGGTCACCAGCTGCAATCAAATCTGCCATCATGACAAGCGCCAATGTACTGAACCTTGCAGGGTTGCCCATTGTTGATCAAAGACTTAATCTCGCGGACGTATATGCAATCGGTGCAGGCCATGTTAACCCTACAAAAGCAAATGACCCGGGGCTCATCTACGACACACAACCTATGGATTACATTCCTTACTTGTGTGGTTTGAATTACACAGAAAAAGAGATACAGATCATCACCCAACAAAAAGTGAACTGCTCTCAAGTCGGGGTCATACCAGAAGCACAGCTCAATTATCCTTCATTTTCTATTAAGATAGGGTCCAATGAGTCTCAGTCTCAGTATTATACAAGGACTGTGAGGAATGTTGGCCCGGCTAGTTCAACTTACAATTTGGATCTTCTTGTACCACATAAAATGGGCATGAGTGTGAATCCTGGGGTGCTTACATTCACAAAGGTGAACCAGAAGATTACGTTCCATGTTGAGTTCATAGCAGAAGATGGTGCTGGGAAGGATGGTGTAGCATTTGGTCAGGGATATTTGAGATGGGTTTCTGATAAGCATAATGTTACTAGCCCAATAGCTGTGATCTTTGACTCTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

626

Amino Acids

66.64

Weight (kDa)

5.71

Isoelectric Point (pI)

33.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 19, 111
Acc65I GGTACC 1 cut(s) 1208
AccB1I GGYRCC 4 cut(s) 647, 733, 1028, 1208
AccB7I CCANNNNNTGG 1 cut(s) 406
AccI GTMKAC 1 cut(s) 1164
AccII CGCG 1 cut(s) 1367
AciI CCGC 3 cut(s) 800, 855, 1367
AclWI GGATC 2 cut(s) 1034, 1674
AcoI YGGCCR 3 cut(s) 264, 640, 750
AcsI RAATTY 5 cut(s) 145, 351, 676, 921, 1101
AcuI CTGAAG 1 cut(s) 804
AcyI GRCGYC 3 cut(s) 290, 734, 1029
AfaI GTAC 4 cut(s) 970, 1210, 1320, 1677
AfiI CCNNNNNNNGG 4 cut(s) 262, 406, 517, 875
AflII CTTAAG 1 cut(s) 1231
AgsI TTSAA 7 cut(s) 128, 547, 821, 1024, 1195, 1477, 1653
AjnI CCWGG 2 cut(s) 1096, 1705
AleI CACNNNNGTG 2 cut(s) 1233, 1727
Alw21I GWGCWC 2 cut(s) 840, 1262
Alw26I GTCTC 2 cut(s) 1605, 1611
AlwI GGATC 2 cut(s) 1034, 1674
AlwNI CAGNNNCTG 2 cut(s) 671, 765
Ama87I CYCGRG 1 cut(s) 1420
AoxI GGCC 8 cut(s) 264, 640, 750, 769, 1124, 1151, 1390, 1639
ApaI GGGCCC 1 cut(s) 1128
ApeKI GCWGC 7 cut(s) 278, 668, 812, 938, 1145, 1244, 1283
ApoI RAATTY 5 cut(s) 145, 351, 676, 921, 1101
Asp700I GAANNNNTTC 1 cut(s) 587
Asp718I GGTACC 1 cut(s) 1208
AspLEI GCGC 4 cut(s) 692, 736, 1031, 1313
AspS9I GGNCC 7 cut(s) 857, 953, 1079, 1124, 1125, 1590, 1640
AsuC2I CCSGG 4 cut(s) 1128, 1421, 1422, 1643
AsuHPI GGTGA 5 cut(s) 446, 1256, 1269, 1498, 1741
AvaI CYCGRG 1 cut(s) 1420
AvaII GGWCC 4 cut(s) 857, 953, 1079, 1590
BaeGI GKGCMC 1 cut(s) 1128
BaeI ACNNNNGTAYC 2 cut(s) 453, 486
BalI TGGCCA 3 cut(s) 266, 642, 752
BanI GGYRCC 4 cut(s) 647, 733, 1028, 1208
BanII GRGCYC 5 cut(s) 599, 625, 1128, 1262, 1428
Bbv12I GWGCWC 2 cut(s) 840, 1262
BbvI GCAGC 7 cut(s) 290, 655, 799, 950, 1132, 1256, 1270
BccI CCATC 6 cut(s) 632, 818, 1307, 1766, 1781, 1812
BciT130I CCWGG 2 cut(s) 1098, 1707
BciVI GTATCC 1 cut(s) 454
BclI TGATCA 3 cut(s) 444, 496, 1348
BcnI CCSGG 4 cut(s) 1128, 1421, 1422, 1643
BcoDI GTCTC 2 cut(s) 1605, 1611
BfaI CTAG 4 cut(s) 660, 1142, 1647, 1845
BfmI CTRYAG 2 cut(s) 615, 1161
BfoI RGCGCY 3 cut(s) 737, 1032, 1314
BfrI CTTAAG 1 cut(s) 1231
BfuAI ACCTGC 2 cut(s) 19, 111
BfuI GTATCC 1 cut(s) 454
BglII AGATCT 1 cut(s) 169
BisI GCNGC 8 cut(s) 279, 669, 801, 813, 939, 1146, 1245, 1284
BlsI GCNGC 8 cut(s) 280, 670, 802, 814, 940, 1147, 1246, 1285
Bme1390I CCNGG 6 cut(s) 1098, 1128, 1421, 1422, 1643, 1707
Bme18I GGWCC 4 cut(s) 857, 953, 1079, 1590
BmeT110I CYCGRG 1 cut(s) 1420
BmgT120I GGNCC 7 cut(s) 857, 953, 1079, 1124, 1125, 1590, 1640
BmiI GGNNCC 8 cut(s) 624, 649, 735, 859, 1030, 1126, 1210, 1591
BmrFI CCNGG 6 cut(s) 1098, 1128, 1421, 1422, 1643, 1707
BmsI GCATC 1 cut(s) 799
BpmI CTGGAG 1 cut(s) 194
BpuEI CTTGAG 3 cut(s) 583, 1238, 1515
BpuMI CCSGG 4 cut(s) 1128, 1421, 1422, 1643
BsaBI GATNNNNATC 1 cut(s) 1499
BsaHI GRCGYC 3 cut(s) 290, 734, 1029
BsaJI CCNNGG 6 cut(s) 400, 643, 1096, 1420, 1421, 1706
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 607, 637
Bsc4I CCNNNNNNNGG 4 cut(s) 262, 406, 517, 875
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 3 cut(s) 207, 469, 1277
Bse3DI GCAATG 4 cut(s) 23, 315, 810, 1239
Bse8I GATNNNNATC 1 cut(s) 1499
BseBI CCWGG 2 cut(s) 1098, 1707
BseDI CCNNGG 6 cut(s) 400, 643, 1096, 1420, 1421, 1706
BseGI GGATG 8 cut(s) 340, 481, 814, 838, 886, 1007, 1137, 1792
BseJI GATNNNNATC 1 cut(s) 1499
BseLI CCNNNNNNNGG 4 cut(s) 262, 406, 517, 875
BseMI GCAATG 4 cut(s) 23, 315, 810, 1239
BseMII CTCAG 3 cut(s) 926, 1616, 1622
BseNI ACTGG 3 cut(s) 207, 469, 1277
BseRI GAGGAG 3 cut(s) 104, 504, 1215
BseSI GKGCMC 1 cut(s) 1128
BseXI GCAGC 7 cut(s) 290, 655, 799, 950, 1132, 1256, 1270
BseYI CCCAGC 1 cut(s) 1778
BsgI GTGCAG 2 cut(s) 957, 1407
Bsh1236I CGCG 1 cut(s) 1367
BshFI GGCC 8 cut(s) 266, 642, 752, 771, 1126, 1153, 1392, 1641
BshNI GGYRCC 4 cut(s) 647, 733, 1028, 1208
BsiHKAI GWGCWC 2 cut(s) 840, 1262
BsiHKCI CYCGRG 1 cut(s) 1420
BsiSI CCGG 5 cut(s) 134, 518, 1128, 1421, 1643
BslFI GGGAC 4 cut(s) 124, 423, 493, 843
BslI CCNNNNNNNGG 4 cut(s) 262, 406, 517, 875
BsmAI GTCTC 2 cut(s) 1605, 1611
BsmFI GGGAC 4 cut(s) 124, 423, 493, 843
BsnI GGCC 8 cut(s) 266, 642, 752, 771, 1126, 1153, 1392, 1641
BsoBI CYCGRG 1 cut(s) 1420
Bsp120I GGGCCC 1 cut(s) 1124
Bsp1286I GDGCHC 6 cut(s) 599, 625, 840, 1128, 1262, 1428
Bsp143I GATC 8 cut(s) 169, 444, 496, 1039, 1348, 1500, 1666, 1860
Bsp19I CCATGG 1 cut(s) 643
BspACI CCGC 3 cut(s) 800, 855, 1367
BspANI GGCC 8 cut(s) 266, 642, 752, 771, 1126, 1153, 1392, 1641
BspCNI CTCAG 3 cut(s) 925, 1615, 1621
BspFNI CGCG 1 cut(s) 1367
BspHI TCATGA 2 cut(s) 163, 1302
BspLI GGNNCC 8 cut(s) 624, 649, 735, 859, 1030, 1126, 1210, 1591
BspMI ACCTGC 2 cut(s) 19, 111
BspPI GGATC 2 cut(s) 1034, 1674
BspT107I GGYRCC 4 cut(s) 647, 733, 1028, 1208
BspTI CTTAAG 1 cut(s) 1231
BsrDI GCAATG 4 cut(s) 23, 315, 810, 1239
BsrFI RCCGGY 1 cut(s) 133
BsrI ACTGG 3 cut(s) 207, 469, 1277
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 6 cut(s) 400, 643, 1096, 1420, 1421, 1706
BssMI GATC 8 cut(s) 169, 444, 496, 1039, 1348, 1500, 1666, 1860
BssNI GRCGYC 3 cut(s) 290, 734, 1029
BssT1I CCWWGG 2 cut(s) 400, 643
Bst2UI CCWGG 2 cut(s) 1098, 1707
Bst4CI ACNGT 2 cut(s) 257, 1627
BstACI GRCGYC 3 cut(s) 290, 734, 1029
BstAFI CTTAAG 1 cut(s) 1231
BstAPI GCANNNNNTGC 3 cut(s) 809, 896, 1385
BstC8I GCNNGC 2 cut(s) 1093, 1390
BstDEI CTNAG 4 cut(s) 912, 1602, 1608, 1872
BstDSI CCRYGG 1 cut(s) 643
BstEII GGTNACC 1 cut(s) 1275
BstF5I GGATG 8 cut(s) 340, 481, 814, 838, 886, 1007, 1137, 1792
BstFNI CGCG 1 cut(s) 1367
BstH2I RGCGCY 3 cut(s) 737, 1032, 1314
BstHHI GCGC 4 cut(s) 692, 736, 1031, 1313
BstKTI GATC 8 cut(s) 172, 447, 499, 1042, 1351, 1503, 1669, 1863
BstMAI GTCTC 2 cut(s) 1605, 1611
BstMBI GATC 8 cut(s) 169, 444, 496, 1039, 1348, 1500, 1666, 1860
BstMWI GCNNNNNNNGC 6 cut(s) 740, 749, 809, 896, 905, 1385
BstNI CCWGG 2 cut(s) 1098, 1707
BstPI GGTNACC 1 cut(s) 1275
BstSCI CCNGG 6 cut(s) 1096, 1126, 1419, 1420, 1641, 1705
BstSFI CTRYAG 2 cut(s) 615, 1161
BstSLI GKGCMC 1 cut(s) 1128
BstUI CGCG 1 cut(s) 1367
BstV1I GCAGC 7 cut(s) 290, 655, 799, 950, 1132, 1256, 1270
BstX2I RGATCY 3 cut(s) 169, 1039, 1666
BstYI RGATCY 3 cut(s) 169, 1039, 1666
BsuI GTATCC 1 cut(s) 454
BsuRI GGCC 8 cut(s) 266, 642, 752, 771, 1126, 1153, 1392, 1641
BtgI CCRYGG 1 cut(s) 643
BtsCI GGATG 8 cut(s) 340, 481, 814, 838, 886, 1007, 1137, 1792
BtsIMutI CAGTG 1 cut(s) 214
BveI ACCTGC 2 cut(s) 19, 111
Cac8I GCNNGC 2 cut(s) 1093, 1390
CaiI CAGNNNCTG 2 cut(s) 671, 765
CciI TCATGA 2 cut(s) 163, 1302
CfoI GCGC 4 cut(s) 692, 736, 1031, 1313
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 7 cut(s) 857, 953, 1079, 1124, 1125, 1590, 1640
Cfr9I CCCGGG 1 cut(s) 1420
CseI GACGC 1 cut(s) 298
Csp6I GTAC 4 cut(s) 969, 1209, 1319, 1676
CviQI GTAC 4 cut(s) 969, 1209, 1319, 1676
DdeI CTNAG 4 cut(s) 912, 1602, 1608, 1872
DinI GGCGCC 2 cut(s) 735, 1030
DpnI GATC 8 cut(s) 171, 446, 498, 1041, 1350, 1502, 1668, 1862
DpnII GATC 8 cut(s) 169, 444, 496, 1039, 1348, 1500, 1666, 1860
EaeI YGGCCR 3 cut(s) 264, 640, 750
Ecl136II GAGCTC 1 cut(s) 1260
Eco130I CCWWGG 2 cut(s) 400, 643
Eco24I GRGCYC 5 cut(s) 599, 625, 1128, 1262, 1428
Eco32I GATATC 1 cut(s) 1117
Eco47I GGWCC 4 cut(s) 857, 953, 1079, 1590
Eco53kI GAGCTC 1 cut(s) 1260
Eco57I CTGAAG 1 cut(s) 804
Eco88I CYCGRG 1 cut(s) 1420
Eco91I GGTNACC 1 cut(s) 1275
EcoICRI GAGCTC 1 cut(s) 1260
EcoO65I GGTNACC 1 cut(s) 1275
EcoRI GAATTC 3 cut(s) 145, 351, 1101
EcoRII CCWGG 2 cut(s) 1096, 1705
EcoRV GATATC 1 cut(s) 1117
EcoT14I CCWWGG 2 cut(s) 400, 643
EcoT38I GRGCYC 5 cut(s) 599, 625, 1128, 1262, 1428
EgeI GGCGCC 2 cut(s) 735, 1030
EheI GGCGCC 2 cut(s) 735, 1030
ErhI CCWWGG 2 cut(s) 400, 643
FaqI GGGAC 4 cut(s) 124, 423, 493, 843
FbaI TGATCA 3 cut(s) 444, 496, 1348
FblI GTMKAC 1 cut(s) 1164
Fnu4HI GCNGC 8 cut(s) 279, 669, 801, 813, 939, 1146, 1245, 1284
FokI GGATG 8 cut(s) 327, 468, 821, 845, 893, 994, 1124, 1799
FriOI GRGCYC 5 cut(s) 599, 625, 1128, 1262, 1428
Fsp4HI GCNGC 8 cut(s) 279, 669, 801, 813, 939, 1146, 1245, 1284
FspBI CTAG 4 cut(s) 660, 1142, 1647, 1845
GlaI GCGC 4 cut(s) 691, 735, 1030, 1312
GluI GCNGC 8 cut(s) 279, 669, 801, 813, 939, 1146, 1245, 1284
GsaI CCCAGC 1 cut(s) 1782
GsuI CTGGAG 1 cut(s) 194
HaeII RGCGCY 3 cut(s) 737, 1032, 1314
HaeIII GGCC 8 cut(s) 266, 642, 752, 771, 1126, 1153, 1392, 1641
HapII CCGG 5 cut(s) 134, 518, 1128, 1421, 1643
HgaI GACGC 1 cut(s) 298
HhaI GCGC 4 cut(s) 692, 736, 1031, 1313
Hin1I GRCGYC 3 cut(s) 290, 734, 1029
Hin6I GCGC 4 cut(s) 690, 734, 1029, 1311
HinP1I GCGC 4 cut(s) 690, 734, 1029, 1311
HincII GTYRAC 3 cut(s) 1135, 1221, 1399
HindII GTYRAC 3 cut(s) 1135, 1221, 1399
HinfI GANTC 4 cut(s) 762, 1598, 1702, 1868
HpaI GTTAAC 1 cut(s) 1399
HpaII CCGG 5 cut(s) 134, 518, 1128, 1421, 1643
HphI GGTGA 5 cut(s) 446, 1256, 1269, 1498, 1741
Hpy166II GTNNAC 7 cut(s) 395, 1135, 1165, 1221, 1399, 1522, 1732
Hpy188I TCNGA 4 cut(s) 915, 1039, 1107, 1828
Hpy188III TCNNGA 7 cut(s) 164, 173, 344, 380, 1074, 1255, 1303
Hpy8I GTNNAC 7 cut(s) 395, 1135, 1165, 1221, 1399, 1522, 1732
HpyAV CCTTC 8 cut(s) 317, 431, 495, 680, 910, 1014, 1578, 1777
HpyCH4III ACNGT 2 cut(s) 257, 1627
HpyCH4IV ACGT 2 cut(s) 1371, 1745
HpyF10VI GCNNNNNNNGC 6 cut(s) 740, 749, 809, 896, 905, 1385
HpyF3I CTNAG 4 cut(s) 912, 1602, 1608, 1872
HpySE526I ACGT 2 cut(s) 1371, 1745
Hsp92I GRCGYC 3 cut(s) 290, 734, 1029
HspAI GCGC 4 cut(s) 690, 734, 1029, 1311
KasI GGCGCC 2 cut(s) 733, 1028
KpnI GGTACC 1 cut(s) 1212
Ksp22I TGATCA 3 cut(s) 444, 496, 1348
KspAI GTTAAC 1 cut(s) 1399
Kzo9I GATC 8 cut(s) 169, 444, 496, 1039, 1348, 1500, 1666, 1860
LmnI GCTCC 4 cut(s) 91, 628, 905, 1054
Lsp1109I GCAGC 7 cut(s) 290, 655, 799, 950, 1132, 1256, 1270
LweI GCATC 1 cut(s) 799
MaeI CTAG 4 cut(s) 660, 1142, 1647, 1845
MaeII ACGT 2 cut(s) 1371, 1745
MaeIII GTNAC 3 cut(s) 539, 1275, 1840
MalI GATC 8 cut(s) 171, 446, 498, 1041, 1350, 1502, 1668, 1862
MboI GATC 8 cut(s) 169, 444, 496, 1039, 1348, 1500, 1666, 1860
MboII GAAGA 5 cut(s) 179, 833, 1661, 1750, 1781
MflI RGATCY 3 cut(s) 169, 1039, 1666
MhlI GDGCHC 6 cut(s) 599, 625, 840, 1128, 1262, 1428
MlsI TGGCCA 3 cut(s) 266, 642, 752
MluNI TGGCCA 3 cut(s) 266, 642, 752
Mly113I GGCGCC 2 cut(s) 734, 1029
MlyI GAGTC 3 cut(s) 771, 1607, 1862
Mox20I TGGCCA 3 cut(s) 266, 642, 752
MroXI GAANNNNTTC 1 cut(s) 587
MscI TGGCCA 3 cut(s) 266, 642, 752
MseI TTAA 7 cut(s) 50, 986, 992, 1232, 1359, 1398, 1581
MslI CAYNNNNRTG 3 cut(s) 1233, 1697, 1727
Msp20I TGGCCA 3 cut(s) 266, 642, 752
MspA1I CMGCKG 6 cut(s) 226, 668, 722, 933, 941, 1283
MspCI CTTAAG 1 cut(s) 1231
MspI CCGG 5 cut(s) 134, 518, 1128, 1421, 1643
MspR9I CCNGG 6 cut(s) 1098, 1128, 1421, 1422, 1643, 1707
MvaI CCWGG 2 cut(s) 1098, 1707
MvnI CGCG 1 cut(s) 1367
MwoI GCNNNNNNNGC 6 cut(s) 740, 749, 809, 896, 905, 1385
NarI GGCGCC 2 cut(s) 734, 1029
NciI CCSGG 4 cut(s) 1128, 1421, 1422, 1643
NcoI CCATGG 1 cut(s) 643
NdeII GATC 8 cut(s) 169, 444, 496, 1039, 1348, 1500, 1666, 1860
NlaIV GGNNCC 8 cut(s) 624, 649, 735, 859, 1030, 1126, 1210, 1591
NmuCI GTSAC 2 cut(s) 539, 1275
OliI CACNNNNGTG 2 cut(s) 1233, 1727
PagI TCATGA 2 cut(s) 163, 1302
PdmI GAANNNNTTC 1 cut(s) 587
PfeI GAWTC 1 cut(s) 1702
PflMI CCANNNNNTGG 1 cut(s) 406
PkrI GCNGC 8 cut(s) 280, 670, 802, 814, 940, 1147, 1246, 1285
PleI GAGTC 3 cut(s) 770, 1606, 1862
PluTI GGCGCC 2 cut(s) 737, 1032
PpsI GAGTC 3 cut(s) 770, 1606, 1862
Psp124BI GAGCTC 1 cut(s) 1262
Psp6I CCWGG 2 cut(s) 1096, 1705
PspEI GGTNACC 1 cut(s) 1275
PspFI CCCAGC 1 cut(s) 1778
PspGI CCWGG 2 cut(s) 1096, 1705
PspN4I GGNNCC 8 cut(s) 624, 649, 735, 859, 1030, 1126, 1210, 1591
PspOMI GGGCCC 1 cut(s) 1124
PspPI GGNCC 7 cut(s) 857, 953, 1079, 1124, 1125, 1590, 1640
PstNI CAGNNNCTG 2 cut(s) 671, 765
PsuI RGATCY 3 cut(s) 169, 1039, 1666
PvuII CAGCTG 6 cut(s) 226, 668, 722, 933, 941, 1283
RsaI GTAC 4 cut(s) 970, 1210, 1320, 1677
RsaNI GTAC 4 cut(s) 969, 1209, 1319, 1676
RseI CAYNNNNRTG 3 cut(s) 1233, 1697, 1727
SacI GAGCTC 1 cut(s) 1262
SaqAI TTAA 7 cut(s) 50, 986, 992, 1232, 1359, 1398, 1581
SatI GCNGC 8 cut(s) 279, 669, 801, 813, 939, 1146, 1245, 1284
Sau3AI GATC 8 cut(s) 169, 444, 496, 1039, 1348, 1500, 1666, 1860
Sau96I GGNCC 7 cut(s) 857, 953, 1079, 1124, 1125, 1590, 1640
SchI GAGTC 3 cut(s) 771, 1607, 1862
ScrFI CCNGG 6 cut(s) 1098, 1128, 1421, 1422, 1643, 1707
SduI GDGCHC 6 cut(s) 599, 625, 840, 1128, 1262, 1428
SfaNI GCATC 1 cut(s) 799
SfcI CTRYAG 2 cut(s) 615, 1161
SfoI GGCGCC 2 cut(s) 735, 1030
SinI GGWCC 4 cut(s) 857, 953, 1079, 1590
SmaI CCCGGG 1 cut(s) 1422
SmiMI CAYNNNNRTG 3 cut(s) 1233, 1697, 1727
SmlI CTYRAG 4 cut(s) 598, 1231, 1253, 1530
SmoI CTYRAG 4 cut(s) 598, 1231, 1253, 1530
SsiI CCGC 3 cut(s) 800, 855, 1367
SspDI GGCGCC 2 cut(s) 733, 1028
SspI AATATT 1 cut(s) 984
SspMI CTAG 4 cut(s) 660, 1142, 1647, 1845
SstI GAGCTC 1 cut(s) 1262
StyD4I CCNGG 6 cut(s) 1096, 1126, 1419, 1420, 1641, 1705
StyI CCWWGG 2 cut(s) 400, 643
TaaI ACNGT 2 cut(s) 257, 1627
TaiI ACGT 2 cut(s) 1374, 1748
TatI WGTACW 1 cut(s) 1318
TauI GCSGC 1 cut(s) 803
TfiI GAWTC 1 cut(s) 1702
Tru1I TTAA 7 cut(s) 50, 986, 992, 1232, 1359, 1398, 1581
Tru9I TTAA 7 cut(s) 50, 986, 992, 1232, 1359, 1398, 1581
TscAI CASTG 1 cut(s) 214
TseFI GTSAC 2 cut(s) 539, 1275
TseI GCWGC 7 cut(s) 278, 668, 812, 938, 1145, 1244, 1283
Tsp45I GTSAC 2 cut(s) 539, 1275
TspDTI ATGAA 9 cut(s) 46, 180, 236, 477, 580, 996, 1059, 1560, 1750
TspMI CCCGGG 1 cut(s) 1420
TspRI CASTG 1 cut(s) 214
Van91I CCANNNNNTGG 1 cut(s) 406
Vha464I CTTAAG 1 cut(s) 1231
VpaK11BI GGWCC 4 cut(s) 857, 953, 1079, 1590
XapI RAATTY 5 cut(s) 145, 351, 676, 921, 1101
XcmI CCANNNNNNNNNTGG 1 cut(s) 641
XmaI CCCGGG 1 cut(s) 1420
XmiI GTMKAC 1 cut(s) 1164
XmnI GAANNNNTTC 1 cut(s) 587
XspI CTAG 4 cut(s) 660, 1142, 1647, 1845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.