MD04G1035800.v1.1

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000302
Physical Location & Seq
Reverse (-)
4011891 .. 4013051
1161 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1035800.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGGAGAACAAGAAAGGTGCAGCAATGCATGTCATATATCTTCTTGGTTTTACTCTGGTCATGTTTTACTTATCAAAGGCAGTTGCACAAGAGGAGCAAAATACCCTCCAAACTTACATTGTTTTTCTTGAAAAGCCGGCCTCCTCGAAATTTTCTGAAGAATCAGATTTAGACAGTTGGTACCAGTCATTTTTGCCTGATACTACAGCGAACTTAAACCAGCAGAGGATCGTTCATGCATACCGCAGTGCTGTCACTGGATTTGCAGCTAAACTGACACCAGAGGAAGTGAAAGCAATGGAAAACAAAGAAGGGTTTTTGTCAGCTCATCTTGAGAAAACGGTTCCTTTGCTAACTACTCATAGTCCTGACTTCTTGGGGTTACACCAAGGATATGGACTATGGAAACAAGCAAATTACGGTGAAGGTGTAATAATAGGACTCTTGGATACAGGCATTGGACCCGATCATCCTTCATTCAGTGACGAAGGAGTATCCCCTCCTCCTGCTAAATGGAAGGGCAAGTGCGACTTCAATGGAACAGTGTGCAACAACAAGCTCATTGGTGCACAAAATTTCGTAGGTGCAGAAGAAGGGAATATTACAGGAAATCCATTTGATCTCGTTGGCCATGGCACCCACACTTCTAGCACCGCTGCTGGAAATTTTGTGGAAGGTGCCAGCGTGTTTGGAGAGGCCAATGGTACGGCTGTTGGCATGGCACCTTACGCTCACTTGGCAATGTACAAAGTCTGTTATGACGATGGTTGTAGCGAAGGCGATATCTTAGCTGGTCTTGATGCCGCTGTTGAAGATGGCTTGGACGTGCTTTCCCTTTCACTTGGAGGCCCCTCACTTCCTTTCTACGATGATGTGATTGCAATCGGTGCATTTGCAGCAATTAAAAAAGGAGTATTTTTCAGCTGTGCGGCTGGAAATTCAGGAGCAGGAAATCGCTATAAATCTTTATCGAATGAGGCCCCATGGATTCTCACAGTTGGAGCAAGTACCACTGACAGAATACTTAAATCGGAAGATGAGAAGCTCAACACCTATGGCAATCAAGCCCTGGAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

360

Amino Acids

38.32

Weight (kDa)

4.83

Isoelectric Point (pI)

31.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 38 - 117 9.9e-11 Peptidase inhibitor I9
Peptidase_S8 PF00082 141 - 338 1.2e-22 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 180
AccB1I GGYRCC 4 cut(s) 180, 635, 677, 721
AciI CCGC 4 cut(s) 244, 654, 804, 929
AclWI GGATC 1 cut(s) 236
AcoI YGGCCR 1 cut(s) 628
AcsI RAATTY 5 cut(s) 149, 574, 664, 937, 1073
AcuI CTGAAG 1 cut(s) 177
AfaI GTAC 4 cut(s) 182, 706, 746, 1009
AgsI TTSAA 3 cut(s) 131, 535, 812
AjiI CACGTC 1 cut(s) 826
AjnI CCWGG 1 cut(s) 1068
AluBI AGCT 6 cut(s) 269, 326, 559, 791, 924, 1045
AluI AGCT 6 cut(s) 269, 326, 559, 791, 924, 1045
Alw21I GWGCWC 1 cut(s) 571
Alw44I GTGCAC 1 cut(s) 567
AlwI GGATC 1 cut(s) 236
AoxI GGCC 5 cut(s) 138, 628, 696, 847, 978
ApaLI GTGCAC 1 cut(s) 567
ApeKI GCWGC 4 cut(s) 20, 266, 656, 896
ApoI RAATTY 5 cut(s) 149, 574, 664, 937, 1073
Asp718I GGTACC 1 cut(s) 180
AspS9I GGNCC 3 cut(s) 461, 848, 979
AsuHPI GGTGA 1 cut(s) 434
AvaII GGWCC 1 cut(s) 461
BaeGI GKGCMC 1 cut(s) 571
BaeI ACNNNNGTAYC 2 cut(s) 696, 729
BalI TGGCCA 1 cut(s) 630
BanI GGYRCC 4 cut(s) 180, 635, 677, 721
Bbv12I GWGCWC 1 cut(s) 571
BbvI GCAGC 4 cut(s) 32, 278, 643, 908
BccI CCATC 2 cut(s) 758, 809
BceAI ACGGC 1 cut(s) 723
BciT130I CCWGG 1 cut(s) 1070
BciVI GTATCC 2 cut(s) 442, 505
BfaI CTAG 1 cut(s) 648
BfmI CTRYAG 1 cut(s) 204
BfuI GTATCC 2 cut(s) 442, 505
BisI GCNGC 6 cut(s) 21, 267, 657, 804, 897, 930
BlsI GCNGC 6 cut(s) 22, 268, 658, 805, 898, 931
Bme1390I CCNGG 1 cut(s) 1070
Bme18I GGWCC 1 cut(s) 461
BmgBI CACGTC 1 cut(s) 826
BmgT120I GGNCC 3 cut(s) 461, 848, 979
BmiI GGNNCC 8 cut(s) 182, 345, 463, 637, 679, 723, 850, 981
BmrFI CCNGG 1 cut(s) 1070
BmsI GCATC 1 cut(s) 790
BpuEI CTTGAG 1 cut(s) 353
BsaBI GATNNNNATC 1 cut(s) 881
BsaJI CCNNGG 4 cut(s) 388, 631, 983, 1068
Bse118I RCCGGY 1 cut(s) 136
Bse1I ACTGG 2 cut(s) 184, 262
Bse3DI GCAATG 3 cut(s) 30, 303, 747
Bse8I GATNNNNATC 1 cut(s) 881
BseBI CCWGG 1 cut(s) 1070
BseDI CCNNGG 4 cut(s) 388, 631, 983, 1068
BseGI GGATG 1 cut(s) 469
BseJI GATNNNNATC 1 cut(s) 881
BseMI GCAATG 3 cut(s) 30, 303, 747
BseNI ACTGG 2 cut(s) 184, 262
BseRI GAGGAG 3 cut(s) 107, 133, 492
BseSI GKGCMC 1 cut(s) 571
BseXI GCAGC 4 cut(s) 32, 278, 643, 908
BsgI GTGCAG 2 cut(s) 39, 606
BshFI GGCC 5 cut(s) 140, 630, 698, 849, 980
BshNI GGYRCC 4 cut(s) 180, 635, 677, 721
BsiHKAI GWGCWC 1 cut(s) 571
BsiSI CCGG 1 cut(s) 137
BsnI GGCC 5 cut(s) 140, 630, 698, 849, 980
Bsp1286I GDGCHC 1 cut(s) 571
Bsp1407I TGTACA 1 cut(s) 744
Bsp143I GATC 3 cut(s) 228, 466, 619
Bsp19I CCATGG 2 cut(s) 631, 983
BspACI CCGC 4 cut(s) 244, 654, 804, 929
BspANI GGCC 5 cut(s) 140, 630, 698, 849, 980
BspLI GGNNCC 8 cut(s) 182, 345, 463, 637, 679, 723, 850, 981
BspPI GGATC 1 cut(s) 236
BspT107I GGYRCC 4 cut(s) 180, 635, 677, 721
BsrDI GCAATG 3 cut(s) 30, 303, 747
BsrFI RCCGGY 1 cut(s) 136
BsrGI TGTACA 1 cut(s) 744
BsrI ACTGG 2 cut(s) 184, 262
BssAI RCCGGY 1 cut(s) 136
BssECI CCNNGG 4 cut(s) 388, 631, 983, 1068
BssMI GATC 3 cut(s) 228, 466, 619
BssT1I CCWWGG 3 cut(s) 388, 631, 983
Bst2UI CCWGG 1 cut(s) 1070
Bst4CI ACNGT 5 cut(s) 176, 343, 422, 544, 997
BstAPI GCANNNNNTGC 1 cut(s) 887
BstAUI TGTACA 1 cut(s) 744
BstC8I GCNNGC 2 cut(s) 138, 682
BstDEI CTNAG 1 cut(s) 787
BstDSI CCRYGG 2 cut(s) 631, 983
BstF5I GGATG 1 cut(s) 469
BstKTI GATC 3 cut(s) 231, 469, 622
BstMBI GATC 3 cut(s) 228, 466, 619
BstMWI GCNNNNNNNGC 4 cut(s) 728, 737, 887, 896
BstNI CCWGG 1 cut(s) 1070
BstNSI RCATGY 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 1068
BstSFI CTRYAG 1 cut(s) 204
BstSLI GKGCMC 1 cut(s) 571
BstV1I GCAGC 4 cut(s) 32, 278, 643, 908
BstXI CCANNNNNNTGG 1 cut(s) 395
BsuI GTATCC 2 cut(s) 442, 505
BsuRI GGCC 5 cut(s) 140, 630, 698, 849, 980
BtgI CCRYGG 2 cut(s) 631, 983
BtrI CACGTC 1 cut(s) 826
BtsCI GGATG 1 cut(s) 469
BtsI GCAGTG 1 cut(s) 253
BtsIMutI CAGTG 5 cut(s) 253, 255, 487, 549, 1011
Cac8I GCNNGC 2 cut(s) 138, 682
Cfr10I RCCGGY 1 cut(s) 136
Cfr13I GGNCC 3 cut(s) 461, 848, 979
Csp6I GTAC 4 cut(s) 181, 705, 745, 1008
CviAII CATG 6 cut(s) 29, 61, 236, 632, 718, 984
CviQI GTAC 4 cut(s) 181, 705, 745, 1008
DdeI CTNAG 1 cut(s) 787
DpnI GATC 3 cut(s) 230, 468, 621
DpnII GATC 3 cut(s) 228, 466, 619
EaeI YGGCCR 1 cut(s) 628
Eco130I CCWWGG 3 cut(s) 388, 631, 983
Eco32I GATATC 1 cut(s) 784
Eco47I GGWCC 1 cut(s) 461
Eco57I CTGAAG 1 cut(s) 177
EcoO109I RGGNCCY 2 cut(s) 848, 979
EcoRII CCWGG 1 cut(s) 1068
EcoRV GATATC 1 cut(s) 784
EcoT14I CCWWGG 3 cut(s) 388, 631, 983
EcoT22I ATGCAT 2 cut(s) 30, 241
ErhI CCWWGG 3 cut(s) 388, 631, 983
FaeI CATG 6 cut(s) 32, 64, 239, 635, 721, 987
FalI AAGNNNNNCTT 2 cut(s) 515, 547
FatI CATG 6 cut(s) 28, 60, 235, 631, 717, 983
Fnu4HI GCNGC 6 cut(s) 21, 267, 657, 804, 897, 930
FokI GGATG 1 cut(s) 456
Fsp4HI GCNGC 6 cut(s) 21, 267, 657, 804, 897, 930
FspBI CTAG 1 cut(s) 648
GluI GCNGC 6 cut(s) 21, 267, 657, 804, 897, 930
HaeIII GGCC 5 cut(s) 140, 630, 698, 849, 980
HapII CCGG 1 cut(s) 137
Hin1II CATG 6 cut(s) 32, 64, 239, 635, 721, 987
HinfI GANTC 3 cut(s) 161, 441, 988
HpaII CCGG 1 cut(s) 137
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 1 cut(s) 569
Hpy188I TCNGA 3 cut(s) 157, 166, 1033
Hpy188III TCNNGA 5 cut(s) 128, 332, 368, 797, 942
Hpy8I GTNNAC 1 cut(s) 569
HpyAV CCTTC 8 cut(s) 305, 419, 482, 483, 511, 587, 668, 770
HpyCH4III ACNGT 5 cut(s) 176, 343, 422, 544, 997
HpyCH4IV ACGT 1 cut(s) 825
HpyF10VI GCNNNNNNNGC 4 cut(s) 728, 737, 887, 896
HpyF3I CTNAG 1 cut(s) 787
HpySE526I ACGT 1 cut(s) 825
Hsp92II CATG 6 cut(s) 32, 64, 239, 635, 721, 987
KpnI GGTACC 1 cut(s) 184
KroI GCCGGC 1 cut(s) 136
KroNI GCCGGC 1 cut(s) 138
Kzo9I GATC 3 cut(s) 228, 466, 619
LmnI GCTCC 3 cut(s) 94, 944, 1001
Lsp1109I GCAGC 4 cut(s) 32, 278, 643, 908
LweI GCATC 1 cut(s) 790
MaeI CTAG 1 cut(s) 648
MaeII ACGT 1 cut(s) 825
MaeIII GTNAC 3 cut(s) 253, 381, 482
MalI GATC 3 cut(s) 230, 468, 621
MboI GATC 3 cut(s) 228, 466, 619
MboII GAAGA 5 cut(s) 32, 170, 602, 824, 1046
MhlI GDGCHC 1 cut(s) 571
MlsI TGGCCA 1 cut(s) 630
MluCI AATT 7 cut(s) 149, 415, 574, 664, 900, 937, 1073
MluNI TGGCCA 1 cut(s) 630
MlyI GAGTC 1 cut(s) 435
MmeI TCCRAC 1 cut(s) 979
Mox20I TGGCCA 1 cut(s) 630
Mph1103I ATGCAT 2 cut(s) 30, 241
MroNI GCCGGC 1 cut(s) 136
MscI TGGCCA 1 cut(s) 630
MseI TTAA 3 cut(s) 215, 903, 1026
Msp20I TGGCCA 1 cut(s) 630
MspA1I CMGCKG 3 cut(s) 656, 806, 924
MspI CCGG 1 cut(s) 137
MspR9I CCNGG 1 cut(s) 1070
MvaI CCWGG 1 cut(s) 1070
MwoI GCNNNNNNNGC 4 cut(s) 728, 737, 887, 896
NaeI GCCGGC 1 cut(s) 138
NcoI CCATGG 2 cut(s) 631, 983
NdeII GATC 3 cut(s) 228, 466, 619
NgoMIV GCCGGC 1 cut(s) 136
NlaIII CATG 6 cut(s) 32, 64, 239, 635, 721, 987
NlaIV GGNNCC 8 cut(s) 182, 345, 463, 637, 679, 723, 850, 981
NmuCI GTSAC 2 cut(s) 253, 482
NsiI ATGCAT 2 cut(s) 30, 241
NspI RCATGY 1 cut(s) 32
PdiI GCCGGC 1 cut(s) 138
PfeI GAWTC 2 cut(s) 161, 988
PkrI GCNGC 6 cut(s) 22, 268, 658, 805, 898, 931
PleI GAGTC 1 cut(s) 435
PpsI GAGTC 1 cut(s) 435
Psp6I CCWGG 1 cut(s) 1068
PspGI CCWGG 1 cut(s) 1068
PspN4I GGNNCC 8 cut(s) 182, 345, 463, 637, 679, 723, 850, 981
PspPI GGNCC 3 cut(s) 461, 848, 979
PvuII CAGCTG 1 cut(s) 924
RsaI GTAC 4 cut(s) 182, 706, 746, 1009
RsaNI GTAC 4 cut(s) 181, 705, 745, 1008
SaqAI TTAA 3 cut(s) 215, 903, 1026
SatI GCNGC 6 cut(s) 21, 267, 657, 804, 897, 930
Sau3AI GATC 3 cut(s) 228, 466, 619
Sau96I GGNCC 3 cut(s) 461, 848, 979
SchI GAGTC 1 cut(s) 435
ScrFI CCNGG 1 cut(s) 1070
SduI GDGCHC 1 cut(s) 571
SfaNI GCATC 1 cut(s) 790
SfcI CTRYAG 1 cut(s) 204
SinI GGWCC 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
Sse9I AATT 7 cut(s) 149, 415, 574, 664, 900, 937, 1073
SsiI CCGC 4 cut(s) 244, 654, 804, 929
SspI AATATT 1 cut(s) 601
SspMI CTAG 1 cut(s) 648
StyD4I CCNGG 1 cut(s) 1068
StyI CCWWGG 3 cut(s) 388, 631, 983
TaaI ACNGT 5 cut(s) 176, 343, 422, 544, 997
TaiI ACGT 1 cut(s) 828
TaqI TCGA 2 cut(s) 146, 971
TasI AATT 7 cut(s) 149, 415, 574, 664, 900, 937, 1073
TatI WGTACW 1 cut(s) 744
TauI GCSGC 2 cut(s) 806, 932
TfiI GAWTC 2 cut(s) 161, 988
Tru1I TTAA 3 cut(s) 215, 903, 1026
Tru9I TTAA 3 cut(s) 215, 903, 1026
TscAI CASTG 5 cut(s) 253, 262, 487, 549, 1018
TseFI GTSAC 2 cut(s) 253, 482
TseI GCWGC 4 cut(s) 20, 266, 656, 896
Tsp45I GTSAC 2 cut(s) 253, 482
TspDTI ATGAA 2 cut(s) 224, 465
TspRI CASTG 5 cut(s) 253, 262, 487, 549, 1018
VneI GTGCAC 1 cut(s) 567
VpaK11BI GGWCC 1 cut(s) 461
XapI RAATTY 5 cut(s) 149, 574, 664, 937, 1073
XceI RCATGY 1 cut(s) 32
XspI CTAG 1 cut(s) 648
Zsp2I ATGCAT 2 cut(s) 30, 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.