Prupe.5G086100_v2.0.a1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000302
Physical Location & Seq
Forward (+)
9774733 .. 9776766
2034 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G086100.1

Sequence Viewer

Length: 1935 bp
ATGCTTTACTCGTACCAAGAAGTGATCAGTGGTTTTGCAGCCAGACTCACTCAGGAGCAAGTGAGAGCAATGAAGGAGATGGATGGCTTTGTGGCAGCACATCCCGAGAGAGTATTTCGTCGGAAAACTACACACACACCCAACTTCTTGGGGCTGCATCAACAAAAAGGTATATGGAAAGAATCCAATTTTGGGAAGGGAGTGATCATTGGAGTACTGGATGGTGGAATTGAGCCCAACCATCCTTCATTTAGTGGTGCAGGAATTCCACCTCCACCTGCAAAATGGAAAGGGAGGTGTGACTTTAACGCGTCAGACTGCAATAACAAGTTAATTGGTGCAAGGGCTTTCAATCTTGCAGCTCAGGCATTGAAAGGAGATCAACCTGAGGCACCAAACGATATAGACGGACATGGGACCCACACAGCAAGCACAGCTGCTGGTGCATTTGTGCAGAATGCTGATGTGCTGGGAAATGCAAAAGCGGCTGTTCAGGATGGTGTTGATGTGATCTCAATCTCTCTTGGCGAAGATTCAGTTCCATTTTTCAATGACAGTACTGCAATAGGCTCATTTGCAGCAATCCAAAAGGGAATATTTGTAAGTTGTTCAGCTGGAAATTCTGGCCCATTTAATGGCACTCTTTCTAATGAAGCCCCCTGGATTCTAACAGTTGGAGCAAGCACCATAGATAGACGTATTGTAGCCACGGCAAAGCTTGGAAATGGAGAAGAATTTGATGGCGAATCTCTCTTTCAGCCAAAAGATTTCCCTTCAACCTTGTTGCCGCTTGTTTATGCTGGAGTAAACGGTAAAGCAAATTCTGCATTGTGCGCTGAAGGATCCTTGAAAGGTCTCAGTGTTAAAGGTAAGGTAGTTTTGTGTGAGCGAGGCGGGGGAATAGGAAGAATTGCCAAGGGAGAGGAAGTGAAAAATGCTGGAGGTGCTGCCATGATTCTTGTGAATGAAGAAACCGATGGCTTCAGTACATCAGCTGATGTTCATGTTCTTCCTGCAACACATGTGAGCCATGCAGCTGGACTGAAGATCAAAGCGTATATAAATTCAACAGCAACACCAACAGCGACAATATTATTCAAAGGAACTGTCATTGGAGACTCATCCACTCCTGCCGTTGCTTCCTTCTCTTCAAGAGGCCCCAGCCTGGCAAGTCCAGGTATTCTGAAACCAGACATTATTGGACCAGGCGTAAGCATTCTTGCTGCATGGCCATTCCCTGTTGACAACACCACAAATTCAAAAGTCAATTTCAACATAATTTCTGGAACTTCAATGTCATGCCCGCATCTCAGTGGCATTGCAGCTTTGCTCAAGAGCTCTCACCCTTACTGGTCACCAGCTGCCATCAAATCTGCTATAATGACTTCTGCTGATCTTCTAAATTTGGAAGGAAAACCAATTCCCGATGAAACACTTCAGCCTGCAGATGTCTTAGCCACCGGTGTAGGCCATGTCAATCCATCAAAGGCAAATGACCCAGGATTAGTATATGATATTCAACCTGATGATTATATCCCTTATCTTTGTGGCTTGGGCTACAAAGACGATGAAGTAAGTATCCTTGTGCACAGACCAATAAAGTGCTCAAAGGTATCAAGCATACCTGAAGGAGAGCTGAACTACCCTTCATTTTCTGTCGCACTGGGACCATCTCAGACATTCACACGAACTGTGACAAATGTTGGTGAGGCATACTCATCTTATGCAGTCAAGGTTAACGCACCAGAAGAAGTCTATGTGAGTATCAATCCCAAAACACTTTACTTCTCAAAGGTGAACCAGAAGCTGTCGTATTCAGTGACATTCAGTCGTATTGGTTCAAGAGGCAAAGCTGGTGAATTCACTCAAGGATTTCTAACATGGGTTTCTGCTAAACACGTCGTTAGGAGTCCAATTTCTGTTAAACTCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

645

Amino Acids

67.69

Weight (kDa)

6.92

Isoelectric Point (pI)

26.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 286
Acc36I ACCTGC 1 cut(s) 286
AccB1I GGYRCC 1 cut(s) 391
AccB7I CCANNNNNTGG 1 cut(s) 635
AccII CGCG 1 cut(s) 311
AciI CCGC 4 cut(s) 485, 788, 894, 1304
AclWI GGATC 2 cut(s) 837, 850
AcoI YGGCCR 1 cut(s) 1229
AcsI RAATTY 8 cut(s) 264, 619, 734, 820, 1063, 1255, 1402, 1859
AcuI CTGAAG 5 cut(s) 858, 967, 1064, 1421, 1647
AfaI GTAC 4 cut(s) 14, 216, 559, 988
AfiI CCNNNNNNNGG 3 cut(s) 192, 635, 1165
AflIII ACRYGT 3 cut(s) 309, 1021, 1897
AgeI ACCGGT 1 cut(s) 1460
AhdI GACNNNNNGTC 1 cut(s) 1827
AjiI CACGTC 1 cut(s) 1900
AjnI CCWGG 5 cut(s) 659, 1164, 1174, 1204, 1498
AjuI GAANNNNNNNTTGG 2 cut(s) 174, 206
Alw21I GWGCWC 3 cut(s) 1340, 1590, 1607
Alw26I GTCTC 2 cut(s) 860, 1110
Alw44I GTGCAC 1 cut(s) 1586
AlwI GGATC 2 cut(s) 837, 850
AlwNI CAGNNNCTG 2 cut(s) 440, 1807
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 4 cut(s) 625, 1156, 1229, 1468
ApaLI GTGCAC 1 cut(s) 1586
ApoI RAATTY 8 cut(s) 264, 619, 734, 820, 1063, 1255, 1402, 1859
ArsI GACNNNNNNTTYG 2 cut(s) 1092, 1124
AsiGI ACCGGT 1 cut(s) 1460
Asp700I GAANNNNTTC 1 cut(s) 1434
AspLEI GCGC 1 cut(s) 836
AspS9I GGNCC 5 cut(s) 417, 626, 1157, 1202, 1667
AsuHPI GGTGA 5 cut(s) 1334, 1347, 1718, 1807, 1868
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 3 cut(s) 417, 1202, 1667
AxyI CCTNAGG 1 cut(s) 387
BaeGI GKGCMC 1 cut(s) 1590
BalI TGGCCA 1 cut(s) 1231
BamHI GGATCC 1 cut(s) 842
BanI GGYRCC 1 cut(s) 391
BanII GRGCYC 2 cut(s) 237, 1340
Bbv12I GWGCWC 3 cut(s) 1340, 1590, 1607
BceAI ACGGC 2 cut(s) 726, 1118
BciT130I CCWGG 5 cut(s) 661, 1166, 1176, 1206, 1500
BciVI GTATCC 1 cut(s) 1589
BclI TGATCA 2 cut(s) 24, 204
BcoDI GTCTC 2 cut(s) 860, 1110
BfmI CTRYAG 1 cut(s) 1443
BfuAI ACCTGC 1 cut(s) 286
BfuI GTATCC 1 cut(s) 1589
BmcAI AGTACT 2 cut(s) 216, 559
Bme1390I CCNGG 5 cut(s) 661, 1166, 1176, 1206, 1500
Bme18I GGWCC 3 cut(s) 417, 1202, 1667
BmeRI GACNNNNNGTC 1 cut(s) 1827
BmeT110I CYCGRG 1 cut(s) 104
BmgBI CACGTC 1 cut(s) 1900
BmgT120I GGNCC 5 cut(s) 417, 626, 1157, 1202, 1667
BmiI GGNNCC 6 cut(s) 393, 418, 419, 844, 1159, 1668
BmrFI CCNGG 5 cut(s) 661, 1166, 1176, 1206, 1500
BmrI ACTGGG 1 cut(s) 1673
BmsI GCATC 2 cut(s) 166, 1315
BmuI ACTGGG 1 cut(s) 1673
BplI GAGNNNNNCTC 2 cut(s) 1700, 1732
BpmI CTGGAG 3 cut(s) 822, 960, 1913
Bpu10I CCTNAGC 1 cut(s) 363
BpuEI CTTGAG 2 cut(s) 1316, 1851
BsaBI GATNNNNATC 1 cut(s) 515
BsaI GGTCTC 1 cut(s) 860
BsaJI CCNNGG 4 cut(s) 659, 708, 915, 1498
BsaWI WCCGGW 1 cut(s) 1460
BsaXI ACNNNNNCTCC 2 cut(s) 912, 942
Bsc4I CCNNNNNNNGG 3 cut(s) 192, 635, 1165
Bse118I RCCGGY 1 cut(s) 1460
Bse1I ACTGG 4 cut(s) 222, 1355, 1668, 1930
Bse21I CCTNAGG 1 cut(s) 387
Bse3DI GCAATG 2 cut(s) 75, 1317
Bse8I GATNNNNATC 1 cut(s) 515
BseBI CCWGG 5 cut(s) 661, 1166, 1176, 1206, 1500
BseDI CCNNGG 4 cut(s) 659, 708, 915, 1498
BseGI GGATG 6 cut(s) 88, 100, 226, 241, 502, 1121
BseJI GATNNNNATC 1 cut(s) 515
BseLI CCNNNNNNNGG 3 cut(s) 192, 635, 1165
BseMI GCAATG 2 cut(s) 75, 1317
BseMII CTCAG 6 cut(s) 65, 377, 378, 871, 1324, 1688
BseNI ACTGG 4 cut(s) 222, 1355, 1668, 1930
BseSI GKGCMC 1 cut(s) 1590
BseYI CCCAGC 2 cut(s) 469, 1160
BsgI GTGCAG 2 cut(s) 279, 473
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 4 cut(s) 627, 1158, 1231, 1470
BshNI GGYRCC 1 cut(s) 391
BshTI ACCGGT 1 cut(s) 1460
BsiHKAI GWGCWC 3 cut(s) 1340, 1590, 1607
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 1 cut(s) 1461
BslFI GGGAC 2 cut(s) 430, 1680
BslI CCNNNNNNNGG 3 cut(s) 192, 635, 1165
BsmAI GTCTC 2 cut(s) 860, 1110
BsmFI GGGAC 2 cut(s) 430, 1680
BsmI GAATGC 2 cut(s) 463, 1215
BsnI GGCC 4 cut(s) 627, 1158, 1231, 1470
Bso31I GGTCTC 1 cut(s) 860
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 4 cut(s) 237, 1340, 1590, 1607
Bsp143I GATC 7 cut(s) 24, 204, 379, 510, 842, 1047, 1393
BspACI CCGC 4 cut(s) 485, 788, 894, 1304
BspANI GGCC 4 cut(s) 627, 1158, 1231, 1470
BspCNI CTCAG 6 cut(s) 64, 376, 379, 870, 1323, 1687
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 6 cut(s) 393, 418, 419, 844, 1159, 1668
BspMAI CTGCAG 1 cut(s) 1447
BspMI ACCTGC 1 cut(s) 286
BspPI GGATC 2 cut(s) 837, 850
BspT107I GGYRCC 1 cut(s) 391
BspTNI GGTCTC 1 cut(s) 860
BsrDI GCAATG 2 cut(s) 75, 1317
BsrFI RCCGGY 1 cut(s) 1460
BsrI ACTGG 4 cut(s) 222, 1355, 1668, 1930
BssAI RCCGGY 1 cut(s) 1460
BssECI CCNNGG 4 cut(s) 659, 708, 915, 1498
BssMI GATC 7 cut(s) 24, 204, 379, 510, 842, 1047, 1393
BssT1I CCWWGG 1 cut(s) 915
Bst2UI CCWGG 5 cut(s) 661, 1166, 1176, 1206, 1500
Bst4CI ACNGT 5 cut(s) 557, 673, 812, 1108, 1693
Bst6I CTCTTC 1 cut(s) 1153
BstAPI GCANNNNNTGC 1 cut(s) 824
BstC8I GCNNGC 4 cut(s) 430, 682, 1304, 1443
BstDEI CTNAG 7 cut(s) 51, 363, 387, 857, 1310, 1453, 1674
BstDSI CCRYGG 1 cut(s) 708
BstEII GGTNACC 1 cut(s) 1353
BstF5I GGATG 6 cut(s) 88, 100, 226, 241, 502, 1121
BstFNI CGCG 1 cut(s) 311
BstHHI GCGC 1 cut(s) 836
BstKTI GATC 7 cut(s) 27, 207, 382, 513, 845, 1050, 1396
BstMAI GTCTC 2 cut(s) 860, 1110
BstMBI GATC 7 cut(s) 24, 204, 379, 510, 842, 1047, 1393
BstMWI GCNNNNNNNGC 7 cut(s) 365, 434, 443, 485, 824, 833, 944
BstNI CCWGG 5 cut(s) 661, 1166, 1176, 1206, 1500
BstNSI RCATGY 1 cut(s) 1025
BstPI GGTNACC 1 cut(s) 1353
BstSCI CCNGG 5 cut(s) 659, 1164, 1174, 1204, 1498
BstSFI CTRYAG 1 cut(s) 1443
BstSLI GKGCMC 1 cut(s) 1590
BstUI CGCG 1 cut(s) 311
BstX2I RGATCY 1 cut(s) 842
BstXI CCANNNNNNTGG 2 cut(s) 148, 1037
BstYI RGATCY 1 cut(s) 842
Bsu36I CCTNAGG 1 cut(s) 387
BsuI GTATCC 1 cut(s) 1589
BsuRI GGCC 4 cut(s) 627, 1158, 1231, 1470
BtgI CCRYGG 1 cut(s) 708
BtrI CACGTC 1 cut(s) 1900
BtsCI GGATG 6 cut(s) 88, 100, 226, 241, 502, 1121
BtsIMutI CAGTG 5 cut(s) 34, 865, 1318, 1661, 1824
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 4 cut(s) 430, 682, 1304, 1443
CaiI CAGNNNCTG 2 cut(s) 440, 1807
CfoI GCGC 1 cut(s) 836
Cfr10I RCCGGY 1 cut(s) 1460
Cfr13I GGNCC 5 cut(s) 417, 626, 1157, 1202, 1667
CseI GACGC 1 cut(s) 300
Csp6I GTAC 4 cut(s) 13, 215, 558, 987
CspAI ACCGGT 1 cut(s) 1460
CviAII CATG 9 cut(s) 413, 952, 1004, 1022, 1031, 1227, 1299, 1472, 1881
CviQI GTAC 4 cut(s) 13, 215, 558, 987
DdeI CTNAG 7 cut(s) 51, 363, 387, 857, 1310, 1453, 1674
DpnI GATC 7 cut(s) 26, 206, 381, 512, 844, 1049, 1395
DpnII GATC 7 cut(s) 24, 204, 379, 510, 842, 1047, 1393
DriI GACNNNNNGTC 1 cut(s) 1827
EaeI YGGCCR 1 cut(s) 1229
Eam1104I CTCTTC 1 cut(s) 1153
Eam1105I GACNNNNNGTC 1 cut(s) 1827
EarI CTCTTC 1 cut(s) 1153
Ecl136II GAGCTC 1 cut(s) 1338
Eco130I CCWWGG 1 cut(s) 915
Eco24I GRGCYC 2 cut(s) 237, 1340
Eco31I GGTCTC 1 cut(s) 860
Eco47I GGWCC 3 cut(s) 417, 1202, 1667
Eco53kI GAGCTC 1 cut(s) 1338
Eco57I CTGAAG 5 cut(s) 858, 967, 1064, 1421, 1647
Eco81I CCTNAGG 1 cut(s) 387
Eco88I CYCGRG 1 cut(s) 104
Eco91I GGTNACC 1 cut(s) 1353
EcoICRI GAGCTC 1 cut(s) 1338
EcoO109I RGGNCCY 2 cut(s) 417, 1157
EcoO65I GGTNACC 1 cut(s) 1353
EcoRI GAATTC 2 cut(s) 264, 1859
EcoRII CCWGG 5 cut(s) 659, 1164, 1174, 1204, 1498
EcoT14I CCWWGG 1 cut(s) 915
EcoT38I GRGCYC 2 cut(s) 237, 1340
ErhI CCWWGG 1 cut(s) 915
FaeI CATG 9 cut(s) 416, 955, 1007, 1025, 1034, 1230, 1302, 1475, 1884
FaqI GGGAC 2 cut(s) 430, 1680
FatI CATG 9 cut(s) 412, 951, 1003, 1021, 1030, 1226, 1298, 1471, 1880
FauI CCCGC 2 cut(s) 887, 1311
FbaI TGATCA 2 cut(s) 24, 204
FokI GGATG 6 cut(s) 87, 95, 228, 233, 509, 1108
FriOI GRGCYC 2 cut(s) 237, 1340
GlaI GCGC 1 cut(s) 835
GsaI CCCAGC 2 cut(s) 473, 1164
GsuI CTGGAG 3 cut(s) 822, 960, 1913
HaeIII GGCC 4 cut(s) 627, 1158, 1231, 1470
HapII CCGG 1 cut(s) 1461
HgaI GACGC 1 cut(s) 300
HhaI GCGC 1 cut(s) 836
Hin1II CATG 9 cut(s) 416, 955, 1007, 1025, 1034, 1230, 1302, 1475, 1884
Hin6I GCGC 1 cut(s) 834
HinP1I GCGC 1 cut(s) 834
HincII GTYRAC 2 cut(s) 1243, 1738
HindII GTYRAC 2 cut(s) 1243, 1738
HindIII AAGCTT 1 cut(s) 716
HinfI GANTC 8 cut(s) 45, 182, 533, 664, 746, 955, 1118, 1909
HpaI GTTAAC 1 cut(s) 1738
HpaII CCGG 1 cut(s) 1461
HphI GGTGA 5 cut(s) 1334, 1347, 1718, 1807, 1868
Hpy166II GTNNAC 5 cut(s) 808, 1243, 1588, 1738, 1798
Hpy188I TCNGA 4 cut(s) 123, 316, 1185, 1677
Hpy188III TCNNGA 8 cut(s) 53, 104, 494, 1152, 1284, 1333, 1424, 1842
Hpy8I GTNNAC 5 cut(s) 808, 1243, 1588, 1738, 1798
Hpy99I CGWCG 2 cut(s) 123, 1904
HpyAV CCTTC 9 cut(s) 67, 190, 255, 783, 833, 1153, 1403, 1622, 1656
HpyCH4III ACNGT 5 cut(s) 557, 673, 812, 1108, 1693
HpyCH4IV ACGT 2 cut(s) 697, 1899
HpyF10VI GCNNNNNNNGC 7 cut(s) 365, 434, 443, 485, 824, 833, 944
HpyF3I CTNAG 7 cut(s) 51, 363, 387, 857, 1310, 1453, 1674
HpySE526I ACGT 2 cut(s) 697, 1899
Hsp92II CATG 9 cut(s) 416, 955, 1007, 1025, 1034, 1230, 1302, 1475, 1884
HspAI GCGC 1 cut(s) 834
KflI GGGWCCC 1 cut(s) 417
Ksp22I TGATCA 2 cut(s) 24, 204
KspAI GTTAAC 1 cut(s) 1738
Kzo9I GATC 7 cut(s) 24, 204, 379, 510, 842, 1047, 1393
LmnI GCTCC 2 cut(s) 55, 677
LweI GCATC 2 cut(s) 166, 1315
MaeII ACGT 2 cut(s) 697, 1899
MaeIII GTNAC 4 cut(s) 299, 1353, 1693, 1819
MalI GATC 7 cut(s) 26, 206, 381, 512, 844, 1049, 1395
MboI GATC 7 cut(s) 24, 204, 379, 510, 842, 1047, 1393
MboII GAAGA 9 cut(s) 542, 743, 918, 980, 1001, 1057, 1140, 1388, 1760
MflI RGATCY 1 cut(s) 842
MhlI GDGCHC 4 cut(s) 237, 1340, 1590, 1607
MlsI TGGCCA 1 cut(s) 1231
MluI ACGCGT 1 cut(s) 309
MluNI TGGCCA 1 cut(s) 1231
MlyI GAGTC 3 cut(s) 39, 1112, 1918
MmeI TCCRAC 2 cut(s) 101, 655
MnlI CCTC 9 cut(s) 282, 288, 382, 884, 916, 935, 1148, 1702, 1838
Mox20I TGGCCA 1 cut(s) 1231
MroXI GAANNNNTTC 1 cut(s) 1434
MscI TGGCCA 1 cut(s) 1231
MseI TTAA 6 cut(s) 306, 332, 633, 864, 1737, 1923
MslI CAYNNNNRTG 1 cut(s) 1311
Msp20I TGGCCA 1 cut(s) 1231
MspA1I CMGCKG 5 cut(s) 437, 614, 995, 1037, 1361
MspI CCGG 1 cut(s) 1461
MspR9I CCNGG 5 cut(s) 661, 1166, 1176, 1206, 1500
Mva1269I GAATGC 2 cut(s) 463, 1215
MvaI CCWGG 5 cut(s) 661, 1166, 1176, 1206, 1500
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 7 cut(s) 365, 434, 443, 485, 824, 833, 944
NdeII GATC 7 cut(s) 24, 204, 379, 510, 842, 1047, 1393
NlaIII CATG 9 cut(s) 416, 955, 1007, 1025, 1034, 1230, 1302, 1475, 1884
NlaIV GGNNCC 6 cut(s) 393, 418, 419, 844, 1159, 1668
NmuCI GTSAC 4 cut(s) 299, 1353, 1693, 1819
NspI RCATGY 1 cut(s) 1025
PaqCI CACCTGC 1 cut(s) 286
PciI ACATGT 1 cut(s) 1021
PctI GAATGC 2 cut(s) 463, 1215
PdmI GAANNNNTTC 1 cut(s) 1434
PfeI GAWTC 5 cut(s) 182, 533, 664, 746, 955
PflMI CCANNNNNTGG 1 cut(s) 635
PinAI ACCGGT 1 cut(s) 1460
PleI GAGTC 3 cut(s) 39, 1112, 1917
PpsI GAGTC 3 cut(s) 39, 1112, 1917
PpuMI RGGWCCY 1 cut(s) 417
PscI ACATGT 1 cut(s) 1021
Psp124BI GAGCTC 1 cut(s) 1340
Psp5II RGGWCCY 1 cut(s) 417
Psp6I CCWGG 5 cut(s) 659, 1164, 1174, 1204, 1498
PspEI GGTNACC 1 cut(s) 1353
PspFI CCCAGC 2 cut(s) 469, 1160
PspGI CCWGG 5 cut(s) 659, 1164, 1174, 1204, 1498
PspN4I GGNNCC 6 cut(s) 393, 418, 419, 844, 1159, 1668
PspPI GGNCC 5 cut(s) 417, 626, 1157, 1202, 1667
PspPPI RGGWCCY 1 cut(s) 417
PstI CTGCAG 1 cut(s) 1447
PstNI CAGNNNCTG 2 cut(s) 440, 1807
PsuI RGATCY 1 cut(s) 842
PvuII CAGCTG 5 cut(s) 437, 614, 995, 1037, 1361
RsaI GTAC 4 cut(s) 14, 216, 559, 988
RsaNI GTAC 4 cut(s) 13, 215, 558, 987
RseI CAYNNNNRTG 1 cut(s) 1311
SacI GAGCTC 1 cut(s) 1340
SaqAI TTAA 6 cut(s) 306, 332, 633, 864, 1737, 1923
Sau3AI GATC 7 cut(s) 24, 204, 379, 510, 842, 1047, 1393
Sau96I GGNCC 5 cut(s) 417, 626, 1157, 1202, 1667
ScaI AGTACT 2 cut(s) 216, 559
SchI GAGTC 3 cut(s) 39, 1112, 1918
ScrFI CCNGG 5 cut(s) 661, 1166, 1176, 1206, 1500
SduI GDGCHC 4 cut(s) 237, 1340, 1590, 1607
SfaNI GCATC 2 cut(s) 166, 1315
SfcI CTRYAG 1 cut(s) 1443
SgrAI CRCCGGYG 1 cut(s) 1460
SinI GGWCC 3 cut(s) 417, 1202, 1667
SmiMI CAYNNNNRTG 1 cut(s) 1311
SmlI CTYRAG 2 cut(s) 1331, 1866
SmoI CTYRAG 2 cut(s) 1331, 1866
SsiI CCGC 4 cut(s) 485, 788, 894, 1304
SspI AATATT 2 cut(s) 597, 1092
SstI GAGCTC 1 cut(s) 1340
StyD4I CCNGG 5 cut(s) 659, 1164, 1174, 1204, 1498
StyI CCWWGG 1 cut(s) 915
TaaI ACNGT 5 cut(s) 557, 673, 812, 1108, 1693
TaiI ACGT 2 cut(s) 700, 1902
TatI WGTACW 3 cut(s) 214, 557, 986
TauI GCSGC 2 cut(s) 488, 790
TfiI GAWTC 5 cut(s) 182, 533, 664, 746, 955
Tru1I TTAA 6 cut(s) 306, 332, 633, 864, 1737, 1923
Tru9I TTAA 6 cut(s) 306, 332, 633, 864, 1737, 1923
TscAI CASTG 5 cut(s) 34, 865, 1318, 1668, 1824
TseFI GTSAC 4 cut(s) 299, 1353, 1693, 1819
Tsp45I GTSAC 4 cut(s) 299, 1353, 1693, 1819
TspDTI ATGAA 8 cut(s) 86, 237, 666, 981, 992, 1443, 1584, 1638
TspGWI ACGGA 1 cut(s) 423
TspRI CASTG 5 cut(s) 34, 865, 1318, 1668, 1824
Van91I CCANNNNNTGG 1 cut(s) 635
VneI GTGCAC 1 cut(s) 1586
VpaK11BI GGWCC 3 cut(s) 417, 1202, 1667
XapI RAATTY 8 cut(s) 264, 619, 734, 820, 1063, 1255, 1402, 1859
XceI RCATGY 1 cut(s) 1025
XcmI CCANNNNNNNNNTGG 1 cut(s) 282
XmnI GAANNNNTTC 1 cut(s) 1434
ZrmI AGTACT 2 cut(s) 216, 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.