Rmu_sc0006398.1_g000003

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006398.1
OG0000302
Physical Location & Seq
Reverse (-)
8894 .. 11167
2274 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006398.1_g000003.1.cds

Sequence Viewer

Length: 2274 bp
atgcagatcatataccttcttggctttagttgcatgttttacttgtcactagcagttgctgtcgaaaaagtggcagtactggaaccacaaggtgagacagatagcatgcaaatttatatagttttggttgaaaggccacccagcaatttcaatgcacaatctgatcatgaagatttagagagttggtaccagtcatttctgccccaaactattgcaaactcaaaccagctcatgaagccacgaatggtttatgcataccacaatgtggccacaggatttgcagcaaagctgactggggaggaagttgaagaaatggagaagaaagatgggtttatctacgctcatccagaacggaatctgcaattgcacactacccacagtccgaacttcttggggttgtaccaagatttgggactttggaaagggtctaattatggtgaaggaatcattattggagttttggatagtggaataacaccaggccatccctcattcagcgatgaaggagtgggacctcctccggctaaatggaaaggcaagtgtgatttcaatggcacattctgcaacaacaagcttattggtgccagaagtttccagggtggtaaaactaccggagctccaccagttgaccttgacggccatggaacccacacgtccagcacagctggaggaaattttgtgaaaggcgccaacgtgtttggaatggcaaagggaacagcctctggcatggcaccttatgctcacttggcaatgtaccaagtttgttctgaacagggttgttctgaaagtgacattgtagctgccatggacactgctattgccgatggagtggacgtgctctccctctcacttggcggtggctcagctcctttctatgcagacggaatcgcagtcggtgcatttggagcaattcagaagggaatttttgttagctgctcagcaggaaatgatggtcctgtatatggttctttatccaatgaggcgccatggattctaaccgtcggagcaagcaccattgacagaagcataaaggcaacagcaaaacttggcaatgggcaggaatatgatggggaatcgttattccaggataaaaattttagttcaaatttgttacctcttgtttatgccggtgcatatagcaatgattcaacagctctgtgcgatgtaggaacccttaatggagttgaagggaaagtggtgctgtgtgaaagaggtggaggaattggaagaattgccaaaggagaagaagtcaaaagagcaggtggtgctgccatgatcctcatgaacgaagacattgatggctatagcaccttagctgatgctcatgtgcttcctgcaacacatgtgagttatgctgcaggtttgagcatcaaagcctacataaactccacctcaacacctatggccacaatcttgttcaaaggcactgtcatcggtgatcaaaatgctcccgcagtcacttccttttcatcaagaggaccaagcctggcgagcacaggaatcttgaaacctgacatcattggtcccggtgttagcatcttagctgcatggcctgtttcagtggacaatggcacagaatctacggcaacatttaacatggtttcaggtacttcaatgtcatgccctcacctgagtggcattgcagctttgctcaagagctcacacccggactggtcaccagctgccatcaagtctgcaattgtgacaaccgctgacatagtaaacctcggaggcaaacctattattgatgaaacacttgctccagcagacatctttgccactggtgcagggcatgttaatcctccaaaagcagatgaccctgggctaatctacgacacaaaaccaacggattacattccctacctgtgcggtttgaactacacagacgatcagatacagataatcacccaacaaacagtgaagtgctctgaagtaggagtcatacctgaagcgcagctaaattatccttcattttctattatattccaggacttgtctcagtctcaagattacacaaggacggtgacaaatgttggaccagctaattcaacttacaacttggatcttaagttaccaggtaaaatcgccatgagtgtgagtccttcgcagcttaagtttacagaagttggccagacaattacatacctagcagagtttatcccggaagatggggcagggaaggatgcaccattttatcagggatatttgagatggttctctgatcagcattctgttactagccccatatctgtgatcttcgacacccagcagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

757

Amino Acids

79.96

Weight (kDa)

4.78

Isoelectric Point (pI)

32.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1250
Acc36I ACCTGC 2 cut(s) 1250, 1349
Acc65I GGTACC 1 cut(s) 186
AccB1I GGYRCC 5 cut(s) 186, 581, 686, 730, 982
AccB7I CCANNNNNTGG 2 cut(s) 265, 409
AciI CCGC 4 cut(s) 855, 1452, 1710, 1869
AclWI GGATC 2 cut(s) 1270, 2070
AcoI YGGCCR 4 cut(s) 267, 637, 1404, 2128
AcsI RAATTY 5 cut(s) 111, 673, 921, 1093, 1105
AcuI CTGAAG 2 cut(s) 1950, 1968
AcyI GRCGYC 2 cut(s) 687, 983
AdeI CACNNNGTG 2 cut(s) 92, 265
AfaI GTAC 5 cut(s) 78, 188, 401, 755, 1609
AfiI CCNNNNNNNGG 4 cut(s) 265, 409, 962, 2168
AflII CTTAAG 2 cut(s) 2066, 2111
AflIII ACRYGT 3 cut(s) 651, 693, 1342
AjiI CACGTC 2 cut(s) 654, 835
AjnI CCWGG 7 cut(s) 478, 594, 1083, 1485, 1819, 1986, 2074
AleI CACNNNNGTG 1 cut(s) 1632
Alw21I GWGCWC 5 cut(s) 619, 840, 1496, 1661, 1928
Alw26I GTCTC 3 cut(s) 89, 2001, 2007
AlwI GGATC 2 cut(s) 1270, 2070
AlwNI CAGNNNCTG 2 cut(s) 59, 722
AoxI GGCC 7 cut(s) 134, 267, 481, 637, 1404, 1550, 2128
ApoI RAATTY 5 cut(s) 111, 673, 921, 1093, 1105
ArsI GACNNNNNNTTYG 2 cut(s) 1413, 1445
Asp718I GGTACC 1 cut(s) 186
AspLEI GCGC 3 cut(s) 689, 985, 1954
AspS9I GGNCC 5 cut(s) 512, 953, 1478, 1523, 2036
AsuC2I CCSGG 3 cut(s) 1527, 1667, 2162
AsuHPI GGTGA 7 cut(s) 104, 449, 1448, 1619, 1668, 1897, 2035
AvaII GGWCC 5 cut(s) 512, 953, 1478, 1523, 2036
BalI TGGCCA 3 cut(s) 269, 1406, 2130
BanI GGYRCC 5 cut(s) 186, 581, 686, 730, 982
BanII GRGCYC 2 cut(s) 619, 1661
BbsI GAAGAC 1 cut(s) 1296
Bbv12I GWGCWC 5 cut(s) 619, 840, 1496, 1661, 1928
BccI CCATC 9 cut(s) 320, 492, 818, 944, 1061, 1292, 1694, 2162, 2205
BceAI ACGGC 2 cut(s) 652, 1599
BcgI CGANNNNNNTGC 2 cut(s) 1414, 1448
BciT130I CCWGG 7 cut(s) 480, 596, 1085, 1487, 1821, 1988, 2076
BclI TGATCA 3 cut(s) 163, 1438, 2221
BcnI CCSGG 3 cut(s) 1527, 1667, 2162
BcoDI GTCTC 3 cut(s) 89, 2001, 2007
BfaI CTAG 3 cut(s) 50, 2147, 2238
BfmI CTRYAG 2 cut(s) 1303, 1356
BfoI RGCGCY 2 cut(s) 690, 986
BfrI CTTAAG 2 cut(s) 2066, 2111
BfuAI ACCTGC 2 cut(s) 1250, 1349
BlpI GCTNAGC 2 cut(s) 862, 937
BmcAI AGTACT 1 cut(s) 78
Bme18I GGWCC 5 cut(s) 512, 953, 1478, 1523, 2036
BmgBI CACGTC 2 cut(s) 654, 835
BmgT120I GGNCC 5 cut(s) 512, 953, 1478, 1523, 2036
BmrI ACTGGG 1 cut(s) 303
BmsI GCATC 4 cut(s) 1309, 1377, 1545, 2173
BmuI ACTGGG 1 cut(s) 303
BpiI GAAGAC 1 cut(s) 1296
BpmI CTGGAG 2 cut(s) 687, 1746
Bpu10I CCTNAGC 1 cut(s) 1312
Bpu1102I GCTNAGC 2 cut(s) 862, 937
BpuEI CTTGAG 2 cut(s) 1637, 1989
BpuMI CCSGG 3 cut(s) 1527, 1667, 2162
BsaHI GRCGYC 2 cut(s) 687, 983
BsaJI CCNNGG 7 cut(s) 595, 640, 804, 986, 1726, 1819, 1820
BsaWI WCCGGW 1 cut(s) 611
Bsc4I CCNNNNNNNGG 4 cut(s) 265, 409, 962, 2168
Bse118I RCCGGY 1 cut(s) 1127
Bse1I ACTGG 6 cut(s) 84, 190, 298, 623, 1676, 1786
Bse3DI GCAATG 4 cut(s) 756, 1057, 1147, 1638
BseBI CCWGG 7 cut(s) 480, 596, 1085, 1487, 1821, 1988, 2076
BseDI CCNNGG 7 cut(s) 595, 640, 804, 986, 1726, 1819, 1820
BseGI GGATG 3 cut(s) 343, 484, 2188
BseLI CCNNNNNNNGG 4 cut(s) 265, 409, 962, 2168
BseMI GCAATG 4 cut(s) 756, 1057, 1147, 1638
BseMII CTCAG 4 cut(s) 876, 951, 1622, 2012
BseNI ACTGG 6 cut(s) 84, 190, 298, 623, 1676, 1786
BseRI GAGGAG 1 cut(s) 507
BseYI CCCAGC 2 cut(s) 140, 2265
BsgI GTGCAG 1 cut(s) 1806
BshFI GGCC 7 cut(s) 136, 269, 483, 639, 1406, 1552, 2130
BshNI GGYRCC 5 cut(s) 186, 581, 686, 730, 982
BsiHKAI GWGCWC 5 cut(s) 619, 840, 1496, 1661, 1928
BsiSI CCGG 6 cut(s) 521, 612, 1128, 1527, 1667, 2162
BslFI GGGAC 3 cut(s) 426, 525, 1509
BslI CCNNNNNNNGG 4 cut(s) 265, 409, 962, 2168
BsmAI GTCTC 3 cut(s) 89, 2001, 2007
BsmFI GGGAC 3 cut(s) 426, 525, 1509
BsmI GAATGC 1 cut(s) 2227
BsnI GGCC 7 cut(s) 136, 269, 483, 639, 1406, 1552, 2130
Bsp1286I GDGCHC 5 cut(s) 619, 840, 1496, 1661, 1928
Bsp143I GATC 8 cut(s) 6, 163, 1275, 1438, 1888, 2062, 2221, 2253
Bsp1720I GCTNAGC 2 cut(s) 862, 937
Bsp19I CCATGG 3 cut(s) 640, 804, 986
BspACI CCGC 4 cut(s) 855, 1452, 1710, 1869
BspANI GGCC 7 cut(s) 136, 269, 483, 639, 1406, 1552, 2130
BspCNI CTCAG 4 cut(s) 875, 950, 1623, 2011
BspHI TCATGA 3 cut(s) 166, 231, 1281
BspMAI CTGCAG 1 cut(s) 1360
BspMI ACCTGC 2 cut(s) 1250, 1349
BspPI GGATC 2 cut(s) 1270, 2070
BspT107I GGYRCC 5 cut(s) 186, 581, 686, 730, 982
BspTI CTTAAG 2 cut(s) 2066, 2111
BsrDI GCAATG 4 cut(s) 756, 1057, 1147, 1638
BsrFI RCCGGY 1 cut(s) 1127
BsrI ACTGG 6 cut(s) 84, 190, 298, 623, 1676, 1786
BssAI RCCGGY 1 cut(s) 1127
BssECI CCNNGG 7 cut(s) 595, 640, 804, 986, 1726, 1819, 1820
BssMI GATC 8 cut(s) 6, 163, 1275, 1438, 1888, 2062, 2221, 2253
BssNI GRCGYC 2 cut(s) 687, 983
BssT1I CCWWGG 3 cut(s) 640, 804, 986
Bst2UI CCWGG 7 cut(s) 480, 596, 1085, 1487, 1821, 1988, 2076
Bst4CI ACNGT 5 cut(s) 380, 1000, 1429, 1918, 2023
BstACI GRCGYC 2 cut(s) 687, 983
BstAFI CTTAAG 2 cut(s) 2066, 2111
BstAPI GCANNNNNTGC 4 cut(s) 561, 737, 896, 1265
BstC8I GCNNGC 3 cut(s) 107, 1009, 1492
BstDEI CTNAG 6 cut(s) 862, 937, 1312, 1540, 1631, 1998
BstDSI CCRYGG 3 cut(s) 640, 804, 986
BstEII GGTNACC 1 cut(s) 1674
BstF5I GGATG 3 cut(s) 343, 484, 2188
BstH2I RGCGCY 2 cut(s) 690, 986
BstHHI GCGC 3 cut(s) 689, 985, 1954
BstKTI GATC 8 cut(s) 9, 166, 1278, 1441, 1891, 2065, 2224, 2256
BstMAI GTCTC 3 cut(s) 89, 2001, 2007
BstMBI GATC 8 cut(s) 6, 163, 1275, 1438, 1888, 2062, 2221, 2253
BstNI CCWGG 7 cut(s) 480, 596, 1085, 1487, 1821, 1988, 2076
BstNSI RCATGY 4 cut(s) 37, 109, 1346, 1796
BstPI GGTNACC 1 cut(s) 1674
BstSFI CTRYAG 2 cut(s) 1303, 1356
BstV2I GAAGAC 1 cut(s) 1296
BstX2I RGATCY 1 cut(s) 2062
BstYI RGATCY 1 cut(s) 2062
BsuRI GGCC 7 cut(s) 136, 269, 483, 639, 1406, 1552, 2130
BtgI CCRYGG 3 cut(s) 640, 804, 986
BtgZI GCGATG 2 cut(s) 513, 1176
BtrI CACGTC 2 cut(s) 654, 835
BtsCI GGATG 3 cut(s) 343, 484, 2188
BtsI GCAGTG 1 cut(s) 810
BtsIMutI CAGTG 5 cut(s) 810, 1425, 1566, 1779, 1923
BveI ACCTGC 2 cut(s) 1250, 1349
Cac8I GCNNGC 3 cut(s) 107, 1009, 1492
CaiI CAGNNNCTG 2 cut(s) 59, 722
CciI TCATGA 3 cut(s) 166, 231, 1281
CfoI GCGC 3 cut(s) 689, 985, 1954
Cfr10I RCCGGY 1 cut(s) 1127
Cfr13I GGNCC 5 cut(s) 512, 953, 1478, 1523, 2036
CsiI ACCWGGT 1 cut(s) 2074
Csp6I GTAC 5 cut(s) 77, 187, 400, 754, 1608
CspCI CAANNNNNGTGG 2 cut(s) 259, 294
CviQI GTAC 5 cut(s) 77, 187, 400, 754, 1608
DdeI CTNAG 6 cut(s) 862, 937, 1312, 1540, 1631, 1998
DinI GGCGCC 2 cut(s) 688, 984
DpnI GATC 8 cut(s) 8, 165, 1277, 1440, 1890, 2064, 2223, 2255
DpnII GATC 8 cut(s) 6, 163, 1275, 1438, 1888, 2062, 2221, 2253
DraIII CACNNNGTG 2 cut(s) 92, 265
EaeI YGGCCR 4 cut(s) 267, 637, 1404, 2128
Ecl136II GAGCTC 2 cut(s) 617, 1659
Eco130I CCWWGG 3 cut(s) 640, 804, 986
Eco24I GRGCYC 2 cut(s) 619, 1661
Eco47I GGWCC 5 cut(s) 512, 953, 1478, 1523, 2036
Eco53kI GAGCTC 2 cut(s) 617, 1659
Eco57I CTGAAG 2 cut(s) 1950, 1968
Eco91I GGTNACC 1 cut(s) 1674
EcoICRI GAGCTC 2 cut(s) 617, 1659
EcoO109I RGGNCCY 1 cut(s) 512
EcoO65I GGTNACC 1 cut(s) 1674
EcoRII CCWGG 7 cut(s) 478, 594, 1083, 1485, 1819, 1986, 2074
EcoT14I CCWWGG 3 cut(s) 640, 804, 986
EcoT22I ATGCAT 1 cut(s) 256
EcoT38I GRGCYC 2 cut(s) 619, 1661
EgeI GGCGCC 2 cut(s) 688, 984
EheI GGCGCC 2 cut(s) 688, 984
ErhI CCWWGG 3 cut(s) 640, 804, 986
FaqI GGGAC 3 cut(s) 426, 525, 1509
FauI CCCGC 1 cut(s) 1459
FbaI TGATCA 3 cut(s) 163, 1438, 2221
FokI GGATG 3 cut(s) 330, 471, 2195
FriOI GRGCYC 2 cut(s) 619, 1661
FspBI CTAG 3 cut(s) 50, 2147, 2238
GlaI GCGC 3 cut(s) 688, 984, 1953
GsaI CCCAGC 2 cut(s) 144, 2269
GsuI CTGGAG 2 cut(s) 687, 1746
HaeII RGCGCY 2 cut(s) 690, 986
HaeIII GGCC 7 cut(s) 136, 269, 483, 639, 1406, 1552, 2130
HapII CCGG 6 cut(s) 521, 612, 1128, 1527, 1667, 2162
HhaI GCGC 3 cut(s) 689, 985, 1954
Hin1I GRCGYC 2 cut(s) 687, 983
Hin6I GCGC 3 cut(s) 687, 983, 1952
HinP1I GCGC 3 cut(s) 687, 983, 1952
HincII GTYRAC 1 cut(s) 628
HindII GTYRAC 1 cut(s) 628
HindIII AAGCTT 1 cut(s) 572
HpaII CCGG 6 cut(s) 521, 612, 1128, 1527, 1667, 2162
HphI GGTGA 7 cut(s) 104, 449, 1448, 1619, 1668, 1897, 2035
Hpy166II GTNNAC 5 cut(s) 628, 832, 1564, 1723, 2118
Hpy188III TCNNGA 8 cut(s) 167, 232, 347, 1282, 1473, 1504, 1654, 2006
Hpy8I GTNNAC 5 cut(s) 628, 832, 1564, 1723, 2118
Hpy99I CGWCG 1 cut(s) 1004
HpyAV CCTTC 8 cut(s) 26, 434, 497, 910, 1181, 1977, 2112, 2173
HpyCH4III ACNGT 5 cut(s) 380, 1000, 1429, 1918, 2023
HpyCH4IV ACGT 3 cut(s) 653, 693, 834
HpyF3I CTNAG 6 cut(s) 862, 937, 1312, 1540, 1631, 1998
HpySE526I ACGT 3 cut(s) 653, 693, 834
Hsp92I GRCGYC 2 cut(s) 687, 983
HspAI GCGC 3 cut(s) 687, 983, 1952
KasI GGCGCC 2 cut(s) 686, 982
KpnI GGTACC 1 cut(s) 190
Ksp22I TGATCA 3 cut(s) 163, 1438, 2221
Kzo9I GATC 8 cut(s) 6, 163, 1275, 1438, 1888, 2062, 2221, 2253
LmnI GCTCC 7 cut(s) 614, 622, 871, 905, 1004, 1453, 1765
LweI GCATC 4 cut(s) 1309, 1377, 1545, 2173
MabI ACCWGGT 1 cut(s) 2074
MaeI CTAG 3 cut(s) 50, 2147, 2238
MaeII ACGT 3 cut(s) 653, 693, 834
MaeIII GTNAC 9 cut(s) 45, 788, 1110, 1456, 1674, 1702, 2023, 2070, 2233
MalI GATC 8 cut(s) 8, 165, 1277, 1440, 1890, 2064, 2223, 2255
MboI GATC 8 cut(s) 6, 163, 1275, 1438, 1888, 2062, 2221, 2253
MboII GAAGA 8 cut(s) 182, 320, 331, 1239, 1256, 1301, 2177, 2248
MfeI CAATTG 2 cut(s) 362, 1698
MflI RGATCY 1 cut(s) 2062
MhlI GDGCHC 5 cut(s) 619, 840, 1496, 1661, 1928
MlsI TGGCCA 3 cut(s) 269, 1406, 2130
MluNI TGGCCA 3 cut(s) 269, 1406, 2130
Mly113I GGCGCC 2 cut(s) 687, 983
MlyI GAGTC 2 cut(s) 1947, 2107
MmeI TCCRAC 2 cut(s) 982, 2014
Mox20I TGGCCA 3 cut(s) 269, 1406, 2130
Mph1103I ATGCAT 1 cut(s) 256
MscI TGGCCA 3 cut(s) 269, 1406, 2130
MseI TTAA 5 cut(s) 1176, 1593, 1797, 2067, 2112
MslI CAYNNNNRTG 3 cut(s) 1632, 2093, 2249
Msp20I TGGCCA 3 cut(s) 269, 1406, 2130
MspA1I CMGCKG 3 cut(s) 665, 1682, 1712
MspCI CTTAAG 2 cut(s) 2066, 2111
MspI CCGG 6 cut(s) 521, 612, 1128, 1527, 1667, 2162
MunI CAATTG 2 cut(s) 362, 1698
Mva1269I GAATGC 1 cut(s) 2227
MvaI CCWGG 7 cut(s) 480, 596, 1085, 1487, 1821, 1988, 2076
NarI GGCGCC 2 cut(s) 687, 983
NciI CCSGG 3 cut(s) 1527, 1667, 2162
NcoI CCATGG 3 cut(s) 640, 804, 986
NdeII GATC 8 cut(s) 6, 163, 1275, 1438, 1888, 2062, 2221, 2253
NmuCI GTSAC 6 cut(s) 45, 788, 1456, 1674, 1702, 2023
NsiI ATGCAT 1 cut(s) 256
NspI RCATGY 4 cut(s) 37, 109, 1346, 1796
OliI CACNNNNGTG 1 cut(s) 1632
PaeI GCATGC 1 cut(s) 109
PagI TCATGA 3 cut(s) 166, 231, 1281
PaqCI CACCTGC 1 cut(s) 1250
PasI CCCWGGG 1 cut(s) 1820
PciI ACATGT 1 cut(s) 1342
PctI GAATGC 1 cut(s) 2227
PfeI GAWTC 8 cut(s) 355, 444, 885, 991, 1073, 1145, 1500, 1577
PflMI CCANNNNNTGG 2 cut(s) 265, 409
PfoI TCCNGGA 3 cut(s) 1083, 1986, 2160
PleI GAGTC 2 cut(s) 1946, 2106
PluTI GGCGCC 2 cut(s) 690, 986
PpsI GAGTC 2 cut(s) 1946, 2106
PpuMI RGGWCCY 1 cut(s) 512
PscI ACATGT 1 cut(s) 1342
Psp124BI GAGCTC 2 cut(s) 619, 1661
Psp5II RGGWCCY 1 cut(s) 512
Psp6I CCWGG 7 cut(s) 478, 594, 1083, 1485, 1819, 1986, 2074
PspEI GGTNACC 1 cut(s) 1674
PspFI CCCAGC 2 cut(s) 140, 2265
PspGI CCWGG 7 cut(s) 478, 594, 1083, 1485, 1819, 1986, 2074
PspPI GGNCC 5 cut(s) 512, 953, 1478, 1523, 2036
PspPPI RGGWCCY 1 cut(s) 512
PstI CTGCAG 1 cut(s) 1360
PstNI CAGNNNCTG 2 cut(s) 59, 722
PsuI RGATCY 1 cut(s) 2062
PvuII CAGCTG 2 cut(s) 665, 1682
RsaI GTAC 5 cut(s) 78, 188, 401, 755, 1609
RsaNI GTAC 5 cut(s) 77, 187, 400, 754, 1608
RseI CAYNNNNRTG 3 cut(s) 1632, 2093, 2249
SacI GAGCTC 2 cut(s) 619, 1661
SaqAI TTAA 5 cut(s) 1176, 1593, 1797, 2067, 2112
Sau3AI GATC 8 cut(s) 6, 163, 1275, 1438, 1888, 2062, 2221, 2253
Sau96I GGNCC 5 cut(s) 512, 953, 1478, 1523, 2036
ScaI AGTACT 1 cut(s) 78
SchI GAGTC 2 cut(s) 1947, 2107
SduI GDGCHC 5 cut(s) 619, 840, 1496, 1661, 1928
SexAI ACCWGGT 1 cut(s) 2074
SfaNI GCATC 4 cut(s) 1309, 1377, 1545, 2173
SfcI CTRYAG 2 cut(s) 1303, 1356
SfoI GGCGCC 2 cut(s) 688, 984
SinI GGWCC 5 cut(s) 512, 953, 1478, 1523, 2036
SmiMI CAYNNNNRTG 3 cut(s) 1632, 2093, 2249
SmlI CTYRAG 4 cut(s) 1652, 2004, 2066, 2111
SmoI CTYRAG 4 cut(s) 1652, 2004, 2066, 2111
SphI GCATGC 1 cut(s) 109
SsiI CCGC 4 cut(s) 855, 1452, 1710, 1869
SspDI GGCGCC 2 cut(s) 686, 982
SspMI CTAG 3 cut(s) 50, 2147, 2238
SstI GAGCTC 2 cut(s) 619, 1661
StyI CCWWGG 3 cut(s) 640, 804, 986
TaaI ACNGT 5 cut(s) 380, 1000, 1429, 1918, 2023
TaiI ACGT 3 cut(s) 656, 696, 837
TaqI TCGA 2 cut(s) 63, 2259
TatI WGTACW 1 cut(s) 76
TfiI GAWTC 8 cut(s) 355, 444, 885, 991, 1073, 1145, 1500, 1577
Tru1I TTAA 5 cut(s) 1176, 1593, 1797, 2067, 2112
Tru9I TTAA 5 cut(s) 1176, 1593, 1797, 2067, 2112
TscAI CASTG 5 cut(s) 817, 1432, 1566, 1786, 1923
TseFI GTSAC 6 cut(s) 45, 788, 1456, 1674, 1702, 2023
Tsp45I GTSAC 6 cut(s) 45, 788, 1456, 1674, 1702, 2023
TspDTI ATGAA 7 cut(s) 183, 248, 516, 1298, 1458, 1764, 1959
TspGWI ACGGA 3 cut(s) 367, 897, 1862
TspRI CASTG 5 cut(s) 817, 1432, 1566, 1786, 1923
Van91I CCANNNNNTGG 2 cut(s) 265, 409
Vha464I CTTAAG 2 cut(s) 2066, 2111
VpaK11BI GGWCC 5 cut(s) 512, 953, 1478, 1523, 2036
XapI RAATTY 5 cut(s) 111, 673, 921, 1093, 1105
XceI RCATGY 4 cut(s) 37, 109, 1346, 1796
XspI CTAG 3 cut(s) 50, 2147, 2238
ZrmI AGTACT 1 cut(s) 78
Zsp2I ATGCAT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.