Rorug07G0058100

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000302
Physical Location & Seq
Forward (+)
4178440 .. 4183065
4626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0058100.1

Sequence Viewer

Length: 1563 bp
ATGTGCAAGGCGGAGAAGGAGAGAAGAGGAGTGATGGGGATAAAGGGGCTGGGAGGAGGAGGAGGTGATAAGCTGAAGAACTCGCTGCTGCAAACGACGTCGTCTTCGTCTTCGAGGTCGAGAATGAAGCTGTGGATGATAAGGGCGACGACGTCGGTTTTGCTGTGGACGTGCGTCGTGCAATTGACGGCGTTGGGGGATATGTGGGGGCCTAGGGTTTTGAAGGGATGGCCGTCCTGCTTCTCTCAGGAGAGTGCCGTGGCCTCGTCGGTTTTGCAGGACCGGATGATTTCCGTTCCGGCCAGAGTTCTTCCGCCAAAGAGGGTTTACAAAAACAATGGCTACCTGATGGTTTCGTGCAATGGGGGACTCAATCAAATGAGAGCAGCGATATGCGACATGGTTGCTATTGCAAGGTATTTGAATGTCACGCTCGTAGTTCCCGAACTTGATAAAACGTCCTTTTGGGCTGATCCCAGTGAGTTTCAAGACATATTTGATGTGGATCATTTCATCACATCCTTGAGAGATGAGGTTCGGATATTGAAAGAGTTGCCTCCCAGGGTTAAGAGAAGAGTGGAACTAGGAATGGTTTATACCATGCCACCAGTTAGTTGGTCTGACATATCTTATTATCATAATCAGATTCTTCCTCTGATACAAAAATACAAAGTTGTACATTTGAATAAAACTGATGCTAGACTGGCCAATAATGGACAACCCTTGGAGATCCAGAAACTTCGATGCCGAGTAAATTTTAGTGCTCTGCGATTCACTTCTCAAATAGAGGAATTGGGTAGAAGGGTCATAAATCTTCTTAGGGCAAATGGTCCTTTCCTAGTTCTTCATCTTAGATATGAAATGGACATGTTGGCATTTTCTGGCTGTACCCAAGGTTGCAACGCTGAGGAGGTGGAAGAGCTGACAAGGATGAGATATGCTTATCCCTGGTGGAAGGAGAAAATAATAAACTCTGACCTGAAAAGGAAAGATGGTTTGTGTCCTTTGACACCAGAAGAAACTGCTCTCACACTGAGTGCACTTGAAATTGATCCCAACATCCAGATCTATATTGCCGCTGGTGAAATCTACGGTGGTGAGAGGAGAATGGCAAGTCTTGCAAAGGCTTTTCCAAAATTGGTCAGAAAGGAGACACTGTTAGAACCGTCAGACCTCAGGTTCTTTCAAAACCACTCCTCCCAAATGGCGGCATTGGATTATCTCGTCTCGTTGGAGAGTGATATCTTTGTTCCCACATATGATGGAAACATGGCTAAAGTTGTTGAAGGCCATCGCAGATTTCTCGGGTTCAAGAAGACAATTCTGCTGGACAGAAGGCTTCTTGTTGATTTGATAGACCAGTACAATGGTGGAGAATTGAATTGGGATGAATTCTCTTCTGCAGTGAAGGAAGCTCATGCAGAACGCATGGGTAACCCAACTAAAAGGTTGATGATCCAAGACAGACCTAAAGAGGAGGACTATTTCTATGCCAACCCAGAAGAGTGTTTGCAATTGTCAGATGATGAGCAATTATCAGATGGGCTATTGAGTAGTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

520

Amino Acids

59.17

Weight (kDa)

8.2

Isoelectric Point (pI)

49.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 115 - 434 4.3e-106 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 101, 155
AciI CCGC 4 cut(s) 11, 314, 1077, 1208
AclWI GGATC 5 cut(s) 467, 513, 724, 1046, 1450
AcoI YGGCCR 3 cut(s) 230, 300, 705
AcsI RAATTY 2 cut(s) 754, 1391
AcuI CTGAAG 1 cut(s) 95
AcyI GRCGYC 2 cut(s) 98, 152
AdeI CACNNNGTG 1 cut(s) 1037
AfaI GTAC 3 cut(s) 678, 889, 1364
AfiI CCNNNNNNNGG 1 cut(s) 1207
AflIII ACRYGT 1 cut(s) 867
AjiI CACGTC 1 cut(s) 171
AjnI CCWGG 2 cut(s) 560, 947
AluBI AGCT 4 cut(s) 73, 130, 922, 1415
AluI AGCT 4 cut(s) 73, 130, 922, 1415
Alw21I GWGCWC 2 cut(s) 766, 1042
Alw26I GTCTC 2 cut(s) 1145, 1231
Alw44I GTGCAC 1 cut(s) 1038
AlwI GGATC 5 cut(s) 467, 513, 724, 1046, 1450
Ama87I CYCGRG 1 cut(s) 1304
AoxI GGCC 6 cut(s) 209, 230, 261, 300, 705, 1288
ApaLI GTGCAC 1 cut(s) 1038
ApeKI GCWGC 3 cut(s) 85, 88, 386
ApoI RAATTY 2 cut(s) 754, 1391
ArsI GACNNNNNNTTYG 4 cut(s) 88, 120, 142, 174
AspA2I CCTAGG 1 cut(s) 212
AspS9I GGNCC 3 cut(s) 209, 280, 830
AsuHPI GGTGA 3 cut(s) 77, 1094, 1109
AvaI CYCGRG 1 cut(s) 1304
AvaII GGWCC 2 cut(s) 280, 830
AvrII CCTAGG 1 cut(s) 212
AxyI CCTNAGG 1 cut(s) 1175
BaeGI GKGCMC 1 cut(s) 1042
BalI TGGCCA 1 cut(s) 707
BbsI GAAGAC 3 cut(s) 96, 102, 1322
Bbv12I GWGCWC 2 cut(s) 766, 1042
BbvCI CCTCAGC 1 cut(s) 906
BbvI GCAGC 3 cut(s) 72, 75, 398
BccI CCATC 7 cut(s) 28, 222, 343, 986, 1256, 1299, 1535
BceAI ACGGC 3 cut(s) 204, 217, 242
BcgI CGANNNNNNTGC 4 cut(s) 386, 420, 1285, 1319
BciT130I CCWGG 2 cut(s) 562, 949
BcoDI GTCTC 2 cut(s) 1145, 1231
BfaI CTAG 4 cut(s) 213, 584, 699, 839
BfmI CTRYAG 1 cut(s) 1401
BglII AGATCT 1 cut(s) 1065
BisI GCNGC 5 cut(s) 86, 89, 387, 1077, 1209
BlnI CCTAGG 1 cut(s) 212
BlsI GCNGC 5 cut(s) 87, 90, 388, 1078, 1210
Bme1390I CCNGG 2 cut(s) 562, 949
Bme18I GGWCC 2 cut(s) 280, 830
BmeT110I CYCGRG 1 cut(s) 1304
BmgBI CACGTC 1 cut(s) 171
BmgT120I GGNCC 3 cut(s) 209, 280, 830
BmiI GGNNCC 1 cut(s) 210
BmrFI CCNGG 2 cut(s) 562, 949
BmrI ACTGGG 1 cut(s) 471
BmsI GCATC 2 cut(s) 685, 734
BmuI ACTGGG 1 cut(s) 471
BoxI GACNNNNGTC 1 cut(s) 173
BpiI GAAGAC 3 cut(s) 96, 102, 1322
Bpu10I CCTNAGC 1 cut(s) 906
BpuEI CTTGAG 1 cut(s) 544
BsaBI GATNNNNATC 1 cut(s) 504
BsaHI GRCGYC 2 cut(s) 98, 152
BsaJI CCNNGG 7 cut(s) 212, 258, 560, 561, 723, 892, 947
BsaWI WCCGGW 1 cut(s) 282
BsaXI ACNNNNNCTCC 4 cut(s) 1142, 1172, 1181, 1211
Bsc4I CCNNNNNNNGG 1 cut(s) 1207
Bse1I ACTGG 4 cut(s) 477, 608, 708, 1360
Bse21I CCTNAGG 1 cut(s) 1175
Bse3DI GCAATG 1 cut(s) 367
Bse8I GATNNNNATC 1 cut(s) 504
BseBI CCWGG 2 cut(s) 562, 949
BseDI CCNNGG 7 cut(s) 212, 258, 560, 561, 723, 892, 947
BseGI GGATG 7 cut(s) 141, 233, 291, 518, 936, 1059, 1393
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 1 cut(s) 1207
BseMI GCAATG 1 cut(s) 367
BseMII CTCAG 4 cut(s) 260, 897, 1025, 1189
BseNI ACTGG 4 cut(s) 477, 608, 708, 1360
BseRI GAGGAG 8 cut(s) 42, 69, 72, 75, 923, 1117, 1186, 1490
BseSI GKGCMC 1 cut(s) 1042
BseXI GCAGC 3 cut(s) 72, 75, 398
BseYI CCCAGC 1 cut(s) 49
BshFI GGCC 6 cut(s) 211, 232, 263, 302, 707, 1290
BsiHKAI GWGCWC 2 cut(s) 766, 1042
BsiHKCI CYCGRG 1 cut(s) 1304
BsiSI CCGG 2 cut(s) 283, 299
BslFI GGGAC 1 cut(s) 381
BslI CCNNNNNNNGG 1 cut(s) 1207
BsmAI GTCTC 2 cut(s) 1145, 1231
BsmBI CGTCTC 1 cut(s) 1231
BsmFI GGGAC 1 cut(s) 381
BsnI GGCC 6 cut(s) 211, 232, 263, 302, 707, 1290
BsoBI CYCGRG 1 cut(s) 1304
Bsp1286I GDGCHC 2 cut(s) 766, 1042
Bsp1407I TGTACA 1 cut(s) 676
Bsp143I GATC 6 cut(s) 472, 505, 729, 1051, 1065, 1455
BspACI CCGC 4 cut(s) 11, 314, 1077, 1208
BspANI GGCC 6 cut(s) 211, 232, 263, 302, 707, 1290
BspCNI CTCAG 4 cut(s) 259, 898, 1026, 1188
BspLI GGNNCC 1 cut(s) 210
BspMAI CTGCAG 1 cut(s) 1405
BspPI GGATC 5 cut(s) 467, 513, 724, 1046, 1450
BspQI GCTCTTC 1 cut(s) 912
BsrDI GCAATG 1 cut(s) 367
BsrGI TGTACA 1 cut(s) 676
BsrI ACTGG 4 cut(s) 477, 608, 708, 1360
BssECI CCNNGG 7 cut(s) 212, 258, 560, 561, 723, 892, 947
BssMI GATC 6 cut(s) 472, 505, 729, 1051, 1065, 1455
BssNI GRCGYC 2 cut(s) 98, 152
BssT1I CCWWGG 3 cut(s) 212, 723, 892
Bst2UI CCWGG 2 cut(s) 562, 949
Bst4CI ACNGT 3 cut(s) 1094, 1158, 1167
Bst6I CTCTTC 5 cut(s) 19, 568, 912, 1402, 1497
BstACI GRCGYC 2 cut(s) 98, 152
BstAPI GCANNNNNTGC 1 cut(s) 1118
BstAUI TGTACA 1 cut(s) 676
BstDEI CTNAG 6 cut(s) 246, 818, 851, 906, 1034, 1175
BstDSI CCRYGG 1 cut(s) 258
BstEII GGTNACC 1 cut(s) 1433
BstF5I GGATG 7 cut(s) 141, 233, 291, 518, 936, 1059, 1393
BstKTI GATC 6 cut(s) 475, 508, 732, 1054, 1068, 1458
BstMAI GTCTC 2 cut(s) 1145, 1231
BstMBI GATC 6 cut(s) 472, 505, 729, 1051, 1065, 1455
BstMWI GCNNNNNNNGC 2 cut(s) 704, 1118
BstNI CCWGG 2 cut(s) 562, 949
BstNSI RCATGY 1 cut(s) 871
BstPAI GACNNNNGTC 1 cut(s) 173
BstPI GGTNACC 1 cut(s) 1433
BstSCI CCNGG 2 cut(s) 560, 947
BstSFI CTRYAG 1 cut(s) 1401
BstSLI GKGCMC 1 cut(s) 1042
BstV1I GCAGC 3 cut(s) 72, 75, 398
BstV2I GAAGAC 3 cut(s) 96, 102, 1322
BstX2I RGATCY 2 cut(s) 729, 1065
BstXI CCANNNNNNTGG 2 cut(s) 615, 1367
BstYI RGATCY 2 cut(s) 729, 1065
Bsu36I CCTNAGG 1 cut(s) 1175
BsuRI GGCC 6 cut(s) 211, 232, 263, 302, 707, 1290
BtgI CCRYGG 1 cut(s) 258
BtgZI GCGATG 1 cut(s) 1277
BtrI CACGTC 1 cut(s) 171
BtsCI GGATG 7 cut(s) 141, 233, 291, 518, 936, 1059, 1393
BtsI GCAGTG 1 cut(s) 1410
BtsIMutI CAGTG 4 cut(s) 484, 1031, 1154, 1410
Cfr13I GGNCC 3 cut(s) 209, 280, 830
CseI GACGC 1 cut(s) 163
Csp6I GTAC 3 cut(s) 677, 888, 1363
CviAII CATG 6 cut(s) 400, 601, 868, 1270, 1418, 1429
CviQI GTAC 3 cut(s) 677, 888, 1363
DdeI CTNAG 6 cut(s) 246, 818, 851, 906, 1034, 1175
DpnI GATC 6 cut(s) 474, 507, 731, 1053, 1067, 1457
DpnII GATC 6 cut(s) 472, 505, 729, 1051, 1065, 1455
DraIII CACNNNGTG 1 cut(s) 1037
EaeI YGGCCR 3 cut(s) 230, 300, 705
Eam1104I CTCTTC 5 cut(s) 19, 568, 912, 1402, 1497
EarI CTCTTC 5 cut(s) 19, 568, 912, 1402, 1497
EciI GGCGGA 2 cut(s) 26, 303
Eco130I CCWWGG 3 cut(s) 212, 723, 892
Eco32I GATATC 1 cut(s) 1243
Eco47I GGWCC 2 cut(s) 280, 830
Eco57I CTGAAG 1 cut(s) 95
Eco81I CCTNAGG 1 cut(s) 1175
Eco88I CYCGRG 1 cut(s) 1304
Eco91I GGTNACC 1 cut(s) 1433
EcoO109I RGGNCCY 1 cut(s) 209
EcoO65I GGTNACC 1 cut(s) 1433
EcoRI GAATTC 1 cut(s) 1391
EcoRII CCWGG 2 cut(s) 560, 947
EcoRV GATATC 1 cut(s) 1243
EcoT14I CCWWGG 3 cut(s) 212, 723, 892
ErhI CCWWGG 3 cut(s) 212, 723, 892
Esp3I CGTCTC 1 cut(s) 1231
FaeI CATG 6 cut(s) 403, 604, 871, 1273, 1421, 1432
FaqI GGGAC 1 cut(s) 381
FatI CATG 6 cut(s) 399, 600, 867, 1269, 1417, 1428
FauNDI CATATG 1 cut(s) 1258
Fnu4HI GCNGC 5 cut(s) 86, 89, 387, 1077, 1209
FokI GGATG 7 cut(s) 148, 240, 298, 505, 943, 1046, 1400
Fsp4HI GCNGC 5 cut(s) 86, 89, 387, 1077, 1209
FspBI CTAG 4 cut(s) 213, 584, 699, 839
GluI GCNGC 5 cut(s) 86, 89, 387, 1077, 1209
GsaI CCCAGC 1 cut(s) 53
HaeIII GGCC 6 cut(s) 211, 232, 263, 302, 707, 1290
HapII CCGG 2 cut(s) 283, 299
HgaI GACGC 1 cut(s) 163
Hin1I GRCGYC 2 cut(s) 98, 152
Hin1II CATG 6 cut(s) 403, 604, 871, 1273, 1421, 1432
HinfI GANTC 3 cut(s) 369, 646, 771
HpaII CCGG 2 cut(s) 283, 299
HphI GGTGA 3 cut(s) 77, 1094, 1109
Hpy166II GTNNAC 3 cut(s) 168, 328, 1040
Hpy188I TCNGA 9 cut(s) 540, 622, 645, 657, 976, 1145, 1171, 1522, 1540
Hpy188III TCNNGA 7 cut(s) 120, 248, 443, 488, 733, 1063, 1312
Hpy8I GTNNAC 3 cut(s) 168, 328, 1040
Hpy99I CGWCG 7 cut(s) 100, 103, 151, 154, 157, 179, 271
HpyAV CCTTC 7 cut(s) 10, 217, 795, 949, 1280, 1329, 1402
HpyCH4III ACNGT 3 cut(s) 1094, 1158, 1167
HpyCH4IV ACGT 4 cut(s) 98, 152, 170, 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 704, 1118
HpyF3I CTNAG 6 cut(s) 246, 818, 851, 906, 1034, 1175
HpySE526I ACGT 4 cut(s) 98, 152, 170, 458
Hsp92I GRCGYC 2 cut(s) 98, 152
Hsp92II CATG 6 cut(s) 403, 604, 871, 1273, 1421, 1432
Kzo9I GATC 6 cut(s) 472, 505, 729, 1051, 1065, 1455
LguI GCTCTTC 1 cut(s) 912
Lsp1109I GCAGC 3 cut(s) 72, 75, 398
LweI GCATC 2 cut(s) 685, 734
MaeI CTAG 4 cut(s) 213, 584, 699, 839
MaeII ACGT 4 cut(s) 98, 152, 170, 458
MaeIII GTNAC 2 cut(s) 427, 1433
MalI GATC 6 cut(s) 474, 507, 731, 1053, 1067, 1457
MboI GATC 6 cut(s) 472, 505, 729, 1051, 1065, 1455
MfeI CAATTG 2 cut(s) 182, 1514
MflI RGATCY 2 cut(s) 729, 1065
MhlI GDGCHC 2 cut(s) 766, 1042
MlsI TGGCCA 1 cut(s) 707
MluNI TGGCCA 1 cut(s) 707
MlyI GAGTC 1 cut(s) 363
MmeI TCCRAC 1 cut(s) 1212
Mox20I TGGCCA 1 cut(s) 707
MscI TGGCCA 1 cut(s) 707
MseI TTAA 1 cut(s) 567
Msp20I TGGCCA 1 cut(s) 707
MspA1I CMGCKG 1 cut(s) 1079
MspI CCGG 2 cut(s) 283, 299
MspR9I CCNGG 2 cut(s) 562, 949
MunI CAATTG 2 cut(s) 182, 1514
MvaI CCWGG 2 cut(s) 562, 949
MwoI GCNNNNNNNGC 2 cut(s) 704, 1118
NdeI CATATG 1 cut(s) 1258
NdeII GATC 6 cut(s) 472, 505, 729, 1051, 1065, 1455
NlaIII CATG 6 cut(s) 403, 604, 871, 1273, 1421, 1432
NlaIV GGNNCC 1 cut(s) 210
NmeAIII GCCGAG 1 cut(s) 773
NmuCI GTSAC 1 cut(s) 427
NspI RCATGY 1 cut(s) 871
PasI CCCWGGG 1 cut(s) 561
PciI ACATGT 1 cut(s) 867
PciSI GCTCTTC 1 cut(s) 912
PcsI WCGNNNNNNNCGW 2 cut(s) 104, 441
PfeI GAWTC 2 cut(s) 646, 771
PflFI GACNNNGTC 2 cut(s) 100, 151
PkrI GCNGC 5 cut(s) 87, 90, 388, 1078, 1210
PleI GAGTC 1 cut(s) 363
PpsI GAGTC 1 cut(s) 363
PscI ACATGT 1 cut(s) 867
PshAI GACNNNNGTC 1 cut(s) 173
Psp6I CCWGG 2 cut(s) 560, 947
PspEI GGTNACC 1 cut(s) 1433
PspFI CCCAGC 1 cut(s) 49
PspGI CCWGG 2 cut(s) 560, 947
PspN4I GGNNCC 1 cut(s) 210
PspPI GGNCC 3 cut(s) 209, 280, 830
PstI CTGCAG 1 cut(s) 1405
PsuI RGATCY 2 cut(s) 729, 1065
PsyI GACNNNGTC 2 cut(s) 100, 151
RsaI GTAC 3 cut(s) 678, 889, 1364
RsaNI GTAC 3 cut(s) 677, 888, 1363
SapI GCTCTTC 1 cut(s) 912
SaqAI TTAA 1 cut(s) 567
SatI GCNGC 5 cut(s) 86, 89, 387, 1077, 1209
Sau3AI GATC 6 cut(s) 472, 505, 729, 1051, 1065, 1455
Sau96I GGNCC 3 cut(s) 209, 280, 830
SchI GAGTC 1 cut(s) 363
ScrFI CCNGG 2 cut(s) 562, 949
SduI GDGCHC 2 cut(s) 766, 1042
SfaNI GCATC 2 cut(s) 685, 734
SfcI CTRYAG 1 cut(s) 1401
SinI GGWCC 2 cut(s) 280, 830
SmlI CTYRAG 1 cut(s) 523
SmoI CTYRAG 1 cut(s) 523
SsiI CCGC 4 cut(s) 11, 314, 1077, 1208
SspMI CTAG 4 cut(s) 213, 584, 699, 839
StyD4I CCNGG 2 cut(s) 560, 947
StyI CCWWGG 3 cut(s) 212, 723, 892
TaaI ACNGT 3 cut(s) 1094, 1158, 1167
TaiI ACGT 4 cut(s) 101, 155, 173, 461
TaqI TCGA 3 cut(s) 113, 119, 742
TatI WGTACW 2 cut(s) 676, 1362
TauI GCSGC 2 cut(s) 1079, 1211
TfiI GAWTC 2 cut(s) 646, 771
Tru1I TTAA 1 cut(s) 567
Tru9I TTAA 1 cut(s) 567
TscAI CASTG 4 cut(s) 484, 1038, 1161, 1410
TseFI GTSAC 1 cut(s) 427
TseI GCWGC 3 cut(s) 85, 88, 386
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 5 cut(s) 140, 502, 836, 873, 1404
TspGWI ACGGA 1 cut(s) 283
TspRI CASTG 4 cut(s) 484, 1038, 1161, 1410
Tth111I GACNNNGTC 2 cut(s) 100, 151
VneI GTGCAC 1 cut(s) 1038
VpaK11BI GGWCC 2 cut(s) 280, 830
XapI RAATTY 2 cut(s) 754, 1391
XceI RCATGY 1 cut(s) 871
XcmI CCANNNNNNNNNTGG 2 cut(s) 612, 1367
XmaJI CCTAGG 1 cut(s) 212
XspI CTAG 4 cut(s) 213, 584, 699, 839
ZraI GACGTC 2 cut(s) 99, 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.