RLG00000028998

Subtilisin-like serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000302
Physical Location & Seq
Forward (+)
31227709 .. 31229510
1802 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028998

Sequence Viewer

Length: 1368 bp
ATGGTTCACTCATACAGCAATGTGGCCACTGGCTTTGCAGCGAAACTGACACTAGAGGAAGTGAAAGCAATGGAAAAGAAAGATGGGTTCGTGCATGCTCATCCAGAGAGGTTTCTGCCTTTGCACACTAGTCGTAGTCCAAACTTCCTGGGGCTGCAACATGGGTTCGGACTTTGGAAAAGGACTAATTATGGTGAAGGTGTGATCGTAGGAGTTTTGGACACTGGGATATCTCCAGATCATCCCTCATCTAGTGATGAAGGAGTGCCACCTCCTCCGGTGAAATGGAAAGGCAAATGCGAGTTCAAGTTCAATGGTACGACAGTGTGCAACAACAAGCTTATTGGTGCAAGAAATTTCCAAGGTGGGAAAGAAACAAAAGAAGTCTCTCCATTTGGTGAAGAAGGCCATGGCACTCTCACTTCTAGCATAGCTGCTGGAAACTTTGTGAAAAGCGCCAGCGTGTTTGGAATGGCAAATGGCACAGCATCTGGCATTGCACTTTATGCTCACTTGGCCATCTACGAGGTCTGCACCGAGGCAGGTTGTGCTGAAAGTGATATGCTAGCAGCTCTTGACACTGCTGTTGAGGACGGTGTGGAAGTGCTCTCACTGTCCATAGGTTTTGCTTCCTCGGTTCCTTTCCATCAAGATGGGGTTGCTATCGGTGCAAATCCCAATAACCAAACTGAGTTCTGTGCCTTGGGATCTCTTGTAAATGTTGAAGGGAAAGTAGTGGTGTGTGAAAGAGGTGGAGGAGTGGGAAGAACTGCCAAAGGGGAAGAAGTGAAAAAAGCAGGAGGTGCTGCCATGATTCTAGTGAACCAAATCAATGATGACTATAGCACCTTAGCTGATGCTCATGTACTTCCAGCAACAAATGTTGGTTTTCAAGCAGGTTTGAGTATCAAAACCTATATAAAATCAACAACAACACCTATAGCCACAGTCTATTCTCCACGCACTGAAATAGGAGATACACTTGCTCCAATGGTTGCTTCTTTCTCATCAAGAGGACCGAGCATAGCTAGCCCCGGAATTTTGAAACCTGACATCATTGGTCCTGGTCTTAATATCCTCGCTGCATGGCCTGTTTCGGTGGATAATGTCACATCATTTGACATTCTTTCAGGTACCTCAGTGTCATGCCCACACCTAAGTGGCATTGCAGCCATGATCAAGAGTTCACACCCGGACTGGTCACCTGCTGCCATTAAATCGGCCATTATGACAACCGCTCATATGCTAAATCTCAAACGCACACCAATTGTTGATAGATCAAATATCTCAGCAGACATCTACAGCACTGGCTCAAGCCATGTCATCTATGACATAAAACCAATGACCCGGGGATCATCTATGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

456

Amino Acids

47.61

Weight (kDa)

6.16

Isoelectric Point (pI)

28.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 65 - 414 8.6e-35 Subtilase family
PA PF02225 229 - 302 4.5e-11 PA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1213
Acc36I ACCTGC 3 cut(s) 533, 887, 1213
Acc65I GGTACC 1 cut(s) 1133
AccB1I GGYRCC 1 cut(s) 1133
AccBSI CCGCTC 1 cut(s) 1238
AciI CCGC 1 cut(s) 1236
AclWI GGATC 2 cut(s) 715, 1360
AcoI YGGCCR 3 cut(s) 24, 516, 1221
AcsI RAATTY 2 cut(s) 355, 1038
AfaI GTAC 3 cut(s) 319, 867, 1135
AgsI TTSAA 5 cut(s) 307, 313, 725, 893, 1045
AhlI ACTAGT 1 cut(s) 128
AjnI CCWGG 2 cut(s) 147, 1063
AjuI GAANNNNNNNTTGG 2 cut(s) 982, 1014
AleI CACNNNNGTG 1 cut(s) 1158
AluBI AGCT 5 cut(s) 340, 434, 572, 854, 1028
AluI AGCT 5 cut(s) 340, 434, 572, 854, 1028
Alw21I GWGCWC 1 cut(s) 609
Alw26I GTCTC 1 cut(s) 391
AlwI GGATC 2 cut(s) 715, 1360
AlwNI CAGNNNCTG 1 cut(s) 491
Ama87I CYCGRG 1 cut(s) 1347
AoxI GGCC 5 cut(s) 24, 406, 516, 1088, 1221
ApeKI GCWGC 8 cut(s) 38, 154, 434, 569, 806, 1082, 1169, 1208
ApoI RAATTY 2 cut(s) 355, 1038
Asp718I GGTACC 1 cut(s) 1133
AspLEI GCGC 1 cut(s) 458
AspS9I GGNCC 2 cut(s) 1016, 1061
AsuC2I CCSGG 4 cut(s) 1035, 1193, 1348, 1349
AsuHPI GGTGA 4 cut(s) 206, 292, 410, 1194
AsuNHI GCTAGC 2 cut(s) 565, 1028
AvaI CYCGRG 1 cut(s) 1347
AvaII GGWCC 2 cut(s) 1016, 1061
BaeI ACNNNNGTAYC 4 cut(s) 309, 342, 1125, 1158
BalI TGGCCA 2 cut(s) 26, 518
BanI GGYRCC 1 cut(s) 1133
Bbv12I GWGCWC 1 cut(s) 609
BbvI GCAGC 8 cut(s) 50, 141, 421, 581, 793, 1069, 1181, 1195
BccI CCATC 4 cut(s) 77, 527, 647, 654
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 2 cut(s) 149, 1065
BclI TGATCA 1 cut(s) 1176
BcnI CCSGG 4 cut(s) 1035, 1193, 1348, 1349
BcoDI GTCTC 1 cut(s) 391
BcuI ACTAGT 1 cut(s) 128
BfaI CTAG 7 cut(s) 53, 129, 252, 426, 566, 818, 1029
BfmI CTRYAG 3 cut(s) 841, 939, 1300
BfoI RGCGCY 1 cut(s) 459
BfuAI ACCTGC 3 cut(s) 533, 887, 1213
BisI GCNGC 8 cut(s) 39, 155, 435, 570, 807, 1083, 1170, 1209
BlsI GCNGC 8 cut(s) 40, 156, 436, 571, 808, 1084, 1171, 1210
Bme1390I CCNGG 6 cut(s) 149, 1035, 1065, 1193, 1348, 1349
Bme18I GGWCC 2 cut(s) 1016, 1061
BmeT110I CYCGRG 1 cut(s) 1347
BmgT120I GGNCC 2 cut(s) 1016, 1061
BmiI GGNNCC 2 cut(s) 639, 1135
BmrFI CCNGG 6 cut(s) 149, 1035, 1065, 1193, 1348, 1349
BmrI ACTGGG 1 cut(s) 234
BmsI GCATC 2 cut(s) 497, 847
BmtI GCTAGC 2 cut(s) 569, 1032
BmuI ACTGGG 1 cut(s) 234
BpmI CTGGAG 1 cut(s) 219
Bpu10I CCTNAGC 1 cut(s) 850
BpuEI CTTGAG 1 cut(s) 1297
BpuMI CCSGG 4 cut(s) 1035, 1193, 1348, 1349
BsaJI CCNNGG 9 cut(s) 148, 361, 409, 537, 633, 702, 1033, 1347, 1348
BsaWI WCCGGW 1 cut(s) 277
BsaXI ACNNNNNCTCC 2 cut(s) 970, 1000
Bse1I ACTGG 4 cut(s) 34, 229, 1202, 1312
Bse3DI GCAATG 4 cut(s) 25, 75, 495, 1164
BseBI CCWGG 2 cut(s) 149, 1065
BseDI CCNNGG 9 cut(s) 148, 361, 409, 537, 633, 702, 1033, 1347, 1348
BseGI GGATG 2 cut(s) 100, 241
BseMI GCAATG 4 cut(s) 25, 75, 495, 1164
BseMII CTCAG 3 cut(s) 681, 1152, 1302
BseNI ACTGG 4 cut(s) 34, 229, 1202, 1312
BseRI GAGGAG 2 cut(s) 264, 771
BseXI GCAGC 8 cut(s) 50, 141, 421, 581, 793, 1069, 1181, 1195
BsgI GTGCAG 1 cut(s) 517
BshFI GGCC 5 cut(s) 26, 408, 518, 1090, 1223
BshNI GGYRCC 1 cut(s) 1133
BsiHKAI GWGCWC 1 cut(s) 609
BsiHKCI CYCGRG 1 cut(s) 1347
BsiSI CCGG 4 cut(s) 278, 1035, 1193, 1348
BsmAI GTCTC 1 cut(s) 391
BsnI GGCC 5 cut(s) 26, 408, 518, 1090, 1223
BsoBI CYCGRG 1 cut(s) 1347
Bsp1286I GDGCHC 1 cut(s) 609
Bsp143I GATC 6 cut(s) 204, 238, 707, 1176, 1277, 1352
Bsp19I CCATGG 1 cut(s) 409
BspACI CCGC 1 cut(s) 1236
BspANI GGCC 5 cut(s) 26, 408, 518, 1090, 1223
BspCNI CTCAG 3 cut(s) 682, 1151, 1301
BspLI GGNNCC 2 cut(s) 639, 1135
BspMI ACCTGC 3 cut(s) 533, 887, 1213
BspOI GCTAGC 2 cut(s) 569, 1032
BspPI GGATC 2 cut(s) 715, 1360
BspT107I GGYRCC 1 cut(s) 1133
BsrBI CCGCTC 1 cut(s) 1238
BsrDI GCAATG 4 cut(s) 25, 75, 495, 1164
BsrI ACTGG 4 cut(s) 34, 229, 1202, 1312
BssECI CCNNGG 9 cut(s) 148, 361, 409, 537, 633, 702, 1033, 1347, 1348
BssMI GATC 6 cut(s) 204, 238, 707, 1176, 1277, 1352
BssT1I CCWWGG 3 cut(s) 361, 409, 702
Bst2UI CCWGG 2 cut(s) 149, 1065
Bst4CI ACNGT 4 cut(s) 325, 596, 615, 949
BstAPI GCANNNNNTGC 3 cut(s) 506, 548, 803
BstC8I GCNNGC 4 cut(s) 96, 460, 567, 1030
BstDEI CTNAG 5 cut(s) 690, 850, 1138, 1157, 1288
BstDSI CCRYGG 1 cut(s) 409
BstEII GGTNACC 1 cut(s) 1200
BstF5I GGATG 2 cut(s) 100, 241
BstH2I RGCGCY 1 cut(s) 459
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 6 cut(s) 207, 241, 710, 1179, 1280, 1355
BstMAI GTCTC 1 cut(s) 391
BstMBI GATC 6 cut(s) 204, 238, 707, 1176, 1277, 1352
BstMWI GCNNNNNNNGC 6 cut(s) 506, 515, 548, 668, 803, 1029
BstNI CCWGG 2 cut(s) 149, 1065
BstNSI RCATGY 1 cut(s) 98
BstPI GGTNACC 1 cut(s) 1200
BstSCI CCNGG 6 cut(s) 147, 1033, 1063, 1191, 1346, 1347
BstSFI CTRYAG 3 cut(s) 841, 939, 1300
BstV1I GCAGC 8 cut(s) 50, 141, 421, 581, 793, 1069, 1181, 1195
BstX2I RGATCY 1 cut(s) 707
BstXI CCANNNNNNTGG 1 cut(s) 653
BstYI RGATCY 1 cut(s) 707
BsuRI GGCC 5 cut(s) 26, 408, 518, 1090, 1223
BtgI CCRYGG 1 cut(s) 409
BtsCI GGATG 2 cut(s) 100, 241
BtsI GCAGTG 1 cut(s) 579
BtsIMutI CAGTG 8 cut(s) 27, 222, 330, 579, 611, 963, 1146, 1305
BveI ACCTGC 3 cut(s) 533, 887, 1213
Cac8I GCNNGC 4 cut(s) 96, 460, 567, 1030
CaiI CAGNNNCTG 1 cut(s) 491
CfoI GCGC 1 cut(s) 458
Cfr13I GGNCC 2 cut(s) 1016, 1061
Cfr9I CCCGGG 1 cut(s) 1347
Csp6I GTAC 3 cut(s) 318, 866, 1134
CspCI CAANNNNNGTGG 2 cut(s) 16, 51
CviAII CATG 9 cut(s) 95, 161, 410, 811, 863, 1086, 1146, 1174, 1319
CviQI GTAC 3 cut(s) 318, 866, 1134
DdeI CTNAG 5 cut(s) 690, 850, 1138, 1157, 1288
DpnI GATC 6 cut(s) 206, 240, 709, 1178, 1279, 1354
DpnII GATC 6 cut(s) 204, 238, 707, 1176, 1277, 1352
EaeI YGGCCR 3 cut(s) 24, 516, 1221
Eco130I CCWWGG 3 cut(s) 361, 409, 702
Eco32I GATATC 1 cut(s) 231
Eco47I GGWCC 2 cut(s) 1016, 1061
Eco88I CYCGRG 1 cut(s) 1347
Eco91I GGTNACC 1 cut(s) 1200
EcoO65I GGTNACC 1 cut(s) 1200
EcoRII CCWGG 2 cut(s) 147, 1063
EcoRV GATATC 1 cut(s) 231
EcoT14I CCWWGG 3 cut(s) 361, 409, 702
ErhI CCWWGG 3 cut(s) 361, 409, 702
FaeI CATG 9 cut(s) 98, 164, 413, 814, 866, 1089, 1149, 1177, 1322
FatI CATG 9 cut(s) 94, 160, 409, 810, 862, 1085, 1145, 1173, 1318
FauNDI CATATG 1 cut(s) 1242
FbaI TGATCA 1 cut(s) 1176
Fnu4HI GCNGC 8 cut(s) 39, 155, 435, 570, 807, 1083, 1170, 1209
FokI GGATG 2 cut(s) 87, 228
Fsp4HI GCNGC 8 cut(s) 39, 155, 435, 570, 807, 1083, 1170, 1209
FspBI CTAG 7 cut(s) 53, 129, 252, 426, 566, 818, 1029
GlaI GCGC 1 cut(s) 457
GluI GCNGC 8 cut(s) 39, 155, 435, 570, 807, 1083, 1170, 1209
GsuI CTGGAG 1 cut(s) 219
HaeII RGCGCY 1 cut(s) 459
HaeIII GGCC 5 cut(s) 26, 408, 518, 1090, 1223
HapII CCGG 4 cut(s) 278, 1035, 1193, 1348
HhaI GCGC 1 cut(s) 458
Hin1II CATG 9 cut(s) 98, 164, 413, 814, 866, 1089, 1149, 1177, 1322
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 1 cut(s) 814
HpaII CCGG 4 cut(s) 278, 1035, 1193, 1348
HphI GGTGA 4 cut(s) 206, 292, 410, 1194
Hpy166II GTNNAC 3 cut(s) 7, 823, 1187
Hpy188I TCNGA 1 cut(s) 170
Hpy188III TCNNGA 6 cut(s) 104, 236, 575, 650, 1011, 1180
Hpy8I GTNNAC 3 cut(s) 7, 823, 1187
HpyAV CCTTC 4 cut(s) 191, 254, 398, 719
HpyCH4III ACNGT 4 cut(s) 325, 596, 615, 949
HpyF10VI GCNNNNNNNGC 6 cut(s) 506, 515, 548, 668, 803, 1029
HpyF3I CTNAG 5 cut(s) 690, 850, 1138, 1157, 1288
Hsp92II CATG 9 cut(s) 98, 164, 413, 814, 866, 1089, 1149, 1177, 1322
HspAI GCGC 1 cut(s) 456
KpnI GGTACC 1 cut(s) 1137
Ksp22I TGATCA 1 cut(s) 1176
Kzo9I GATC 6 cut(s) 204, 238, 707, 1176, 1277, 1352
LmnI GCTCC 1 cut(s) 991
Lsp1109I GCAGC 8 cut(s) 50, 141, 421, 581, 793, 1069, 1181, 1195
LweI GCATC 2 cut(s) 497, 847
MaeI CTAG 7 cut(s) 53, 129, 252, 426, 566, 818, 1029
MaeIII GTNAC 2 cut(s) 1108, 1200
MalI GATC 6 cut(s) 206, 240, 709, 1178, 1279, 1354
MbiI CCGCTC 1 cut(s) 1238
MboI GATC 6 cut(s) 204, 238, 707, 1176, 1277, 1352
MboII GAAGA 3 cut(s) 413, 777, 794
MfeI CAATTG 1 cut(s) 1266
MflI RGATCY 1 cut(s) 707
MhlI GDGCHC 1 cut(s) 609
MlsI TGGCCA 2 cut(s) 26, 518
MluCI AATT 4 cut(s) 187, 355, 1038, 1266
MluNI TGGCCA 2 cut(s) 26, 518
Mox20I TGGCCA 2 cut(s) 26, 518
MscI TGGCCA 2 cut(s) 26, 518
MseI TTAA 2 cut(s) 1071, 1215
MslI CAYNNNNRTG 2 cut(s) 651, 1158
Msp20I TGGCCA 2 cut(s) 26, 518
MspI CCGG 4 cut(s) 278, 1035, 1193, 1348
MspR9I CCNGG 6 cut(s) 149, 1035, 1065, 1193, 1348, 1349
MunI CAATTG 1 cut(s) 1266
MvaI CCWGG 2 cut(s) 149, 1065
MwoI GCNNNNNNNGC 6 cut(s) 506, 515, 548, 668, 803, 1029
NciI CCSGG 4 cut(s) 1035, 1193, 1348, 1349
NcoI CCATGG 1 cut(s) 409
NdeI CATATG 1 cut(s) 1242
NdeII GATC 6 cut(s) 204, 238, 707, 1176, 1277, 1352
NheI GCTAGC 2 cut(s) 565, 1028
NlaIII CATG 9 cut(s) 98, 164, 413, 814, 866, 1089, 1149, 1177, 1322
NlaIV GGNNCC 2 cut(s) 639, 1135
NmuCI GTSAC 2 cut(s) 1108, 1200
NspI RCATGY 1 cut(s) 98
OliI CACNNNNGTG 1 cut(s) 1158
PaeI GCATGC 1 cut(s) 98
PaqCI CACCTGC 1 cut(s) 1213
PfeI GAWTC 1 cut(s) 814
PkrI GCNGC 8 cut(s) 40, 156, 436, 571, 808, 1084, 1171, 1210
Psp6I CCWGG 2 cut(s) 147, 1063
PspEI GGTNACC 1 cut(s) 1200
PspGI CCWGG 2 cut(s) 147, 1063
PspN4I GGNNCC 2 cut(s) 639, 1135
PspPI GGNCC 2 cut(s) 1016, 1061
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 1 cut(s) 707
RsaI GTAC 3 cut(s) 319, 867, 1135
RsaNI GTAC 3 cut(s) 318, 866, 1134
RseI CAYNNNNRTG 2 cut(s) 651, 1158
SaqAI TTAA 2 cut(s) 1071, 1215
SatI GCNGC 8 cut(s) 39, 155, 435, 570, 807, 1083, 1170, 1209
Sau3AI GATC 6 cut(s) 204, 238, 707, 1176, 1277, 1352
Sau96I GGNCC 2 cut(s) 1016, 1061
ScrFI CCNGG 6 cut(s) 149, 1035, 1065, 1193, 1348, 1349
SduI GDGCHC 1 cut(s) 609
SfaNI GCATC 2 cut(s) 497, 847
SfcI CTRYAG 3 cut(s) 841, 939, 1300
SinI GGWCC 2 cut(s) 1016, 1061
SmaI CCCGGG 1 cut(s) 1349
SmiMI CAYNNNNRTG 2 cut(s) 651, 1158
SmlI CTYRAG 1 cut(s) 1312
SmoI CTYRAG 1 cut(s) 1312
SpeI ACTAGT 1 cut(s) 128
SphI GCATGC 1 cut(s) 98
Sse9I AATT 4 cut(s) 187, 355, 1038, 1266
SsiI CCGC 1 cut(s) 1236
SspMI CTAG 7 cut(s) 53, 129, 252, 426, 566, 818, 1029
StyD4I CCNGG 6 cut(s) 147, 1033, 1063, 1191, 1346, 1347
StyI CCWWGG 3 cut(s) 361, 409, 702
TaaI ACNGT 4 cut(s) 325, 596, 615, 949
TaqII GACCGA 1 cut(s) 1033
TasI AATT 4 cut(s) 187, 355, 1038, 1266
TatI WGTACW 1 cut(s) 865
TfiI GAWTC 1 cut(s) 814
Tru1I TTAA 2 cut(s) 1071, 1215
Tru9I TTAA 2 cut(s) 1071, 1215
TscAI CASTG 8 cut(s) 34, 229, 330, 586, 618, 970, 1146, 1312
TseFI GTSAC 2 cut(s) 1108, 1200
TseI GCWGC 8 cut(s) 38, 154, 434, 569, 806, 1082, 1169, 1208
Tsp45I GTSAC 2 cut(s) 1108, 1200
TspDTI ATGAA 1 cut(s) 273
TspMI CCCGGG 1 cut(s) 1347
TspRI CASTG 8 cut(s) 34, 229, 330, 586, 618, 970, 1146, 1312
VpaK11BI GGWCC 2 cut(s) 1016, 1061
XapI RAATTY 2 cut(s) 355, 1038
XceI RCATGY 1 cut(s) 98
XmaI CCCGGG 1 cut(s) 1347
XspI CTAG 7 cut(s) 53, 129, 252, 426, 566, 818, 1029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.