Prupe.5G085100_v2.0.a1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000302
Physical Location & Seq
Forward (+)
9723154 .. 9725385
2232 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G085100.1

Sequence Viewer

Length: 2232 bp
ATGTTTTATCGATCACTAGGACTTGTACAAGAGGAGCAGAATAGCCTGCAAACATATATTGTTTGGGTTGAAAAGCCGGTCTCTCGGAATATCTTTTCACAATCTCATGAAGGTTTAGAGAGCTGGTATCAGTCATTTTTGCCTGAAACGACTGTAAACTCAAACGAGCAGAAGAGTTCGCGAATTGTTCATGCATACCGCAATGTGGGCACCGGTTTTGCAGCGAAACTGACACCTGAGGAAGTAAAAGCAATGGAAAAGAAACAAGGGTTTGTCTCAGCTCGTCCGGAGAGAATTCTGCCTCTGCAAACTACTCACACTCCTGACTTCTTGGGGTTGCACCAAGGATACGGACTTTGGAAACAAACAAACTATGGTGAAGGTGTGATCATTGGAGTTTTGGATACCGGGATTGGAGCGGGACATCCTTCCTTTAGTGATGAAGGAGTACCACCTCCTCCGGCTAGATGGAAAGGAAAATGCGAGTTCAATGCTACATTGTGCAATAACAAGCTTATTGGTGCAAAAAATTTTGTAGGCTCAGGCAAAGGTAATACCACAGACAGCCCTCCAGTTGATAACCATGGCCATGGCACACACACGTCTAGTACCGCTGCAGGAAATTTTGTGGCAGGTGCCAGCGTTTTTGGAGAGGCCAATGGCACAGCAGTTGGCATGGCACCTTATGCTCACTTGGCAATGTACAAAGTGTGTGCTGGGGGTGGTTGTGCTGACGGTGATATTTTAGCTGCTCTTGATGCCGCTGTTGAAGATGGCGTGGACGTGCTCTCCCTCTCCCTTGGTGGCGAATCACTTCCTTTCTATGAGGATGTTATTGCAATTGGTGCATTTGCGGCAATTCAAAAGGGAATATTTTTCAGCTGTGCAGCTGGAAATTCTGGTCCTTCCTATAACTCTCTATCAAATGAGGCCCCATGGATTCTGACAGTTGGAGCAAGCACCACTGACAGAATATTAAAATCAAAAGCGCAGATTGGAGATGATAAAAACAACCATTTTGATGGAAAATCTTTGTCCCAGCCTGAAGATTTCGATTCAACAGTTTCGCTACCTCTTGTTTATGCAGGCTCAGTTGGCAACCAGCCATCAGCTTCCTGCAAGGCAGGGTCTCTTGAAAACGTTGAAGGGAAAATAGTGTTGTGTGAGACAGGTGGAGGAGTAACAAATATTGCCAAAGGGGAAGAAGTGAAAAGAGCTGGTGGTGCTGCCATGATTCTCATGAACCAAGAGACTGATGGCTTTAGCACCTTAGCTGAATCTCATGTGCTCCCCTCGACGCATGTGAGTTATGCTGCAGGGTTGCAAATTAAGTCATATATAAGCTCAACCTCAAGTCCTACAGCCAAAATCTTGTTCAACGGCACTGTCATCGGAGATGCACTTGCTCCCAAAGTCGCTTCCTTTTCGTCTAGAGGACCAAGCTCTGCAAGCCCTGGTATTCTGAAACCTGACATCATTGGACCCGGAGTTAGCATCCTAGCAGCCTGGCCTGTTGCCGTGGACCAGACACAATCTAAAGCAACGTTTAACATAATTTCGGGTACCTCAATGTCAACTCCTCACCTGAGTGGCATTGCAGCCTTGCTCAAGAGCTCACACCCTGACTGGTCTCCAGCTGCTATTAAGTCTGCCATCATGACAACAGCCAACGTCCTAAACCTCGAAGGCAAGCCCATTGTTGATCAAACGCTTGAGCCCGCGGACATCTTTGCAACCGGTGCAGGCCATGTTAACCCGTCAAAAGCTAATGACCCGGGGCTTGTCTATGACACACAACCAAAGGATTACATTCCCTACTTGTGTGGTTTGAATTACACAGACCAACAGATACAGCTCATCACCAAACAAGCAGTGATCTGCTCTCAAGTAGAAGCCATACCAGAAGCACAGCTAAACTACCCTTCATTTTCCATTAAAATAAGGTCCAATGAGACTCAGTCTCAGTATTATACAAGGACAGTGAGGAACGTTGGCCCGGCTAGTTCAACTTACAACTTGGATCTTCTTGTACCACATAAAATGGGGATGAGTGTGAACCCTCAGGTGCTCACATTCACAGAGGTAAACCAGGAGCTTACGTTCCATGTGGAGTTTATCGCAGAAGATGGCGCTGGCAAGGATGGCGTACCGATTTCTCAGGGATATTTGAGATGGTTTTCTGATAAGTATAATGTTACTAGTCCAATAGCTGTGGTCTTTGAATCTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

744

Amino Acids

78.66

Weight (kDa)

5.43

Isoelectric Point (pI)

39.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 623
Acc36I ACCTGC 1 cut(s) 623
Acc65I GGTACC 1 cut(s) 1562
AccB1I GGYRCC 4 cut(s) 209, 635, 679, 1562
AccBSI CCGCTC 1 cut(s) 419
AccII CGCG 2 cut(s) 181, 1721
AccIII TCCGGA 1 cut(s) 286
AciI CCGC 7 cut(s) 199, 419, 612, 762, 854, 1719, 1721
AclI AACGTT 3 cut(s) 1140, 1544, 1989
AclWI GGATC 1 cut(s) 2028
AcoI YGGCCR 1 cut(s) 586
AcsI RAATTY 4 cut(s) 294, 529, 622, 895
AcuI CTGAAG 1 cut(s) 1065
AfaI GTAC 7 cut(s) 27, 450, 610, 704, 1564, 2031, 2148
AfiI CCNNNNNNNGG 1 cut(s) 205
AflIII ACRYGT 1 cut(s) 600
AgeI ACCGGT 2 cut(s) 212, 1736
AhlI ACTAGT 1 cut(s) 2198
AjiI CACGTC 2 cut(s) 603, 784
AjnI CCWGG 3 cut(s) 1453, 1505, 2088
AjuI GAANNNNNNNTTGG 2 cut(s) 1358, 1390
AleI CACNNNNGTG 1 cut(s) 1587
AloI GAACNNNNNNTCC 2 cut(s) 2084, 2116
Alw21I GWGCWC 4 cut(s) 789, 1290, 1616, 2070
Alw26I GTCTC 8 cut(s) 85, 280, 1134, 1160, 1244, 1635, 1946, 1965
AlwI GGATC 1 cut(s) 2028
Ama87I CYCGRG 1 cut(s) 1774
Aor13HI TCCGGA 1 cut(s) 286
AoxI GGCC 6 cut(s) 586, 654, 930, 1508, 1744, 1993
ApeKI GCWGC 9 cut(s) 221, 614, 749, 887, 1226, 1313, 1502, 1598, 1637
ApoI RAATTY 4 cut(s) 294, 529, 622, 895
AsiGI ACCGGT 2 cut(s) 212, 1736
Asp700I GAANNNNTTC 1 cut(s) 813
Asp718I GGTACC 1 cut(s) 1562
AspLEI GCGC 2 cut(s) 991, 2132
AspS9I GGNCC 7 cut(s) 902, 931, 1436, 1481, 1522, 1944, 1994
AsuC2I CCSGG 5 cut(s) 409, 1485, 1775, 1776, 1997
AsuHPI GGTGA 4 cut(s) 389, 749, 1574, 1852
AvaI CYCGRG 1 cut(s) 1774
AvaII GGWCC 5 cut(s) 902, 1436, 1481, 1522, 1944
AxyI CCTNAGG 2 cut(s) 237, 2061
BaeGI GKGCMC 1 cut(s) 212
BalI TGGCCA 1 cut(s) 588
BanI GGYRCC 4 cut(s) 209, 635, 679, 1562
BanII GRGCYC 2 cut(s) 1616, 1719
Bbv12I GWGCWC 4 cut(s) 789, 1290, 1616, 2070
BbvI GCAGC 9 cut(s) 233, 601, 736, 899, 1213, 1300, 1514, 1610, 1624
BccI CCATC 9 cut(s) 462, 767, 1016, 1114, 1250, 1661, 2120, 2135, 2166
BceAI ACGGC 2 cut(s) 1396, 1502
BcgI CGANNNNNNTGC 4 cut(s) 473, 507, 1372, 1406
BciT130I CCWGG 3 cut(s) 1455, 1507, 2090
BciVI GTATCC 2 cut(s) 341, 397
BclI TGATCA 2 cut(s) 387, 1702
BcnI CCSGG 5 cut(s) 409, 1485, 1775, 1776, 1997
BcoDI GTCTC 8 cut(s) 85, 280, 1134, 1160, 1244, 1635, 1946, 1965
BcuI ACTAGT 1 cut(s) 2198
BfaI CTAG 8 cut(s) 17, 465, 606, 1431, 1499, 2001, 2199, 2230
BfmI CTRYAG 3 cut(s) 615, 1314, 1359
BfoI RGCGCY 1 cut(s) 2133
BfuAI ACCTGC 1 cut(s) 623
BfuI GTATCC 2 cut(s) 341, 397
Bme1390I CCNGG 8 cut(s) 409, 1455, 1485, 1507, 1775, 1776, 1997, 2090
Bme18I GGWCC 5 cut(s) 902, 1436, 1481, 1522, 1944
BmeT110I CYCGRG 1 cut(s) 1774
BmgBI CACGTC 2 cut(s) 603, 784
BmgT120I GGNCC 7 cut(s) 902, 931, 1436, 1481, 1522, 1944, 1994
BmiI GGNNCC 6 cut(s) 211, 637, 681, 933, 1483, 1564
BmrFI CCNGG 8 cut(s) 409, 1455, 1485, 1507, 1775, 1776, 1997, 2090
BmsI GCATC 3 cut(s) 748, 1387, 1503
BpmI CTGGAG 2 cut(s) 555, 1617
Bpu10I CCTNAGC 2 cut(s) 541, 1270
BpuEI CTTGAG 4 cut(s) 1336, 1592, 1733, 1869
BpuMI CCSGG 5 cut(s) 409, 1485, 1775, 1776, 1997
Bsa29I ATCGAT 1 cut(s) 10
BsaI GGTCTC 3 cut(s) 85, 1134, 1635
BsaWI WCCGGW 3 cut(s) 212, 286, 1736
BsaXI ACNNNNNCTCC 6 cut(s) 304, 334, 773, 803, 2084, 2114
Bsc4I CCNNNNNNNGG 1 cut(s) 205
Bse118I RCCGGY 3 cut(s) 76, 212, 1736
Bse1I ACTGG 2 cut(s) 572, 1631
Bse21I CCTNAGG 2 cut(s) 237, 2061
Bse3DI GCAATG 4 cut(s) 208, 258, 705, 1593
BseAI TCCGGA 1 cut(s) 286
BseBI CCWGG 3 cut(s) 1455, 1507, 2090
BseCI ATCGAT 1 cut(s) 10
BseGI GGATG 5 cut(s) 424, 835, 1494, 2052, 2146
BseLI CCNNNNNNNGG 1 cut(s) 205
BseMI GCAATG 4 cut(s) 208, 258, 705, 1593
BseMII CTCAG 9 cut(s) 228, 291, 555, 1104, 1577, 1970, 1976, 2075, 2171
BseNI ACTGG 2 cut(s) 572, 1631
BseRI GAGGAG 4 cut(s) 47, 447, 1191, 1569
BseSI GKGCMC 1 cut(s) 212
BseXI GCAGC 9 cut(s) 233, 601, 736, 899, 1213, 1300, 1514, 1610, 1624
BseYI CCCAGC 2 cut(s) 716, 1038
BsgI GTGCAG 2 cut(s) 906, 1761
Bsh1236I CGCG 2 cut(s) 181, 1721
BshFI GGCC 6 cut(s) 588, 656, 932, 1510, 1746, 1995
BshNI GGYRCC 4 cut(s) 209, 635, 679, 1562
BshTI ACCGGT 2 cut(s) 212, 1736
BshVI ATCGAT 1 cut(s) 10
BsiHKAI GWGCWC 4 cut(s) 789, 1290, 1616, 2070
BsiHKCI CYCGRG 1 cut(s) 1774
BsiSI CCGG 9 cut(s) 77, 213, 287, 408, 461, 1485, 1737, 1775, 1997
BslFI GGGAC 2 cut(s) 435, 1021
BslI CCNNNNNNNGG 1 cut(s) 205
BsmAI GTCTC 8 cut(s) 85, 280, 1134, 1160, 1244, 1635, 1946, 1965
BsmFI GGGAC 2 cut(s) 435, 1021
BsnI GGCC 6 cut(s) 588, 656, 932, 1510, 1746, 1995
Bso31I GGTCTC 3 cut(s) 85, 1134, 1635
BsoBI CYCGRG 1 cut(s) 1774
Bsp1286I GDGCHC 6 cut(s) 212, 789, 1290, 1616, 1719, 2070
Bsp13I TCCGGA 1 cut(s) 286
Bsp1407I TGTACA 2 cut(s) 25, 702
Bsp143I GATC 5 cut(s) 11, 387, 1702, 1875, 2020
Bsp19I CCATGG 3 cut(s) 583, 589, 935
Bsp68I TCGCGA 1 cut(s) 181
BspACI CCGC 7 cut(s) 199, 419, 612, 762, 854, 1719, 1721
BspANI GGCC 6 cut(s) 588, 656, 932, 1510, 1746, 1995
BspCNI CTCAG 9 cut(s) 229, 290, 554, 1103, 1578, 1969, 1975, 2074, 2170
BspDI ATCGAT 1 cut(s) 10
BspEI TCCGGA 1 cut(s) 286
BspFNI CGCG 2 cut(s) 181, 1721
BspHI TCATGA 3 cut(s) 106, 1239, 1656
BspLI GGNNCC 6 cut(s) 211, 637, 681, 933, 1483, 1564
BspMAI CTGCAG 2 cut(s) 619, 1318
BspMI ACCTGC 1 cut(s) 623
BspPI GGATC 1 cut(s) 2028
BspT107I GGYRCC 4 cut(s) 209, 635, 679, 1562
BspTNI GGTCTC 3 cut(s) 85, 1134, 1635
BsrBI CCGCTC 1 cut(s) 419
BsrDI GCAATG 4 cut(s) 208, 258, 705, 1593
BsrFI RCCGGY 3 cut(s) 76, 212, 1736
BsrGI TGTACA 2 cut(s) 25, 702
BsrI ACTGG 2 cut(s) 572, 1631
BssAI RCCGGY 3 cut(s) 76, 212, 1736
BssMI GATC 5 cut(s) 11, 387, 1702, 1875, 2020
BssT1I CCWWGG 5 cut(s) 343, 583, 589, 799, 935
Bst2UI CCWGG 3 cut(s) 1455, 1507, 2090
Bst4CI ACNGT 6 cut(s) 154, 737, 949, 1063, 1387, 1981
Bst6I CTCTTC 1 cut(s) 167
BstAPI GCANNNNNTGC 3 cut(s) 686, 845, 1739
BstAUI TGTACA 2 cut(s) 25, 702
BstC8I GCNNGC 9 cut(s) 47, 640, 958, 1087, 1450, 1691, 1719, 1744, 2134
BstDSI CCRYGG 5 cut(s) 583, 589, 935, 1518, 1719
BstF5I GGATG 5 cut(s) 424, 835, 1494, 2052, 2146
BstFNI CGCG 2 cut(s) 181, 1721
BstH2I RGCGCY 1 cut(s) 2133
BstHHI GCGC 2 cut(s) 991, 2132
BstKTI GATC 5 cut(s) 14, 390, 1705, 1878, 2023
BstMAI GTCTC 8 cut(s) 85, 280, 1134, 1160, 1244, 1635, 1946, 1965
BstMBI GATC 5 cut(s) 11, 387, 1702, 1875, 2020
BstNI CCWGG 3 cut(s) 1455, 1507, 2090
BstNSI RCATGY 1 cut(s) 1304
BstSCI CCNGG 8 cut(s) 407, 1453, 1483, 1505, 1773, 1774, 1995, 2088
BstSFI CTRYAG 3 cut(s) 615, 1314, 1359
BstSLI GKGCMC 1 cut(s) 212
BstUI CGCG 2 cut(s) 181, 1721
BstV1I GCAGC 9 cut(s) 233, 601, 736, 899, 1213, 1300, 1514, 1610, 1624
BstX2I RGATCY 1 cut(s) 2020
BstXI CCANNNNNNTGG 2 cut(s) 590, 1022
BstYI RGATCY 1 cut(s) 2020
Bsu15I ATCGAT 1 cut(s) 10
Bsu36I CCTNAGG 2 cut(s) 237, 2061
BsuI GTATCC 2 cut(s) 341, 397
BsuRI GGCC 6 cut(s) 588, 656, 932, 1510, 1746, 1995
BsuTUI ATCGAT 1 cut(s) 10
BtgI CCRYGG 5 cut(s) 583, 589, 935, 1518, 1719
BtrI CACGTC 2 cut(s) 603, 784
BtsCI GGATG 5 cut(s) 424, 835, 1494, 2052, 2146
BtsI GCAGTG 1 cut(s) 1878
BtsIMutI CAGTG 4 cut(s) 963, 1383, 1878, 1986
BtuMI TCGCGA 1 cut(s) 181
BveI ACCTGC 1 cut(s) 623
Cac8I GCNNGC 9 cut(s) 47, 640, 958, 1087, 1450, 1691, 1719, 1744, 2134
CciI TCATGA 3 cut(s) 106, 1239, 1656
CfoI GCGC 2 cut(s) 991, 2132
Cfr10I RCCGGY 3 cut(s) 76, 212, 1736
Cfr13I GGNCC 7 cut(s) 902, 931, 1436, 1481, 1522, 1944, 1994
Cfr42I CCGCGG 1 cut(s) 1722
Cfr9I CCCGGG 1 cut(s) 1774
ClaI ATCGAT 1 cut(s) 10
CseI GACGC 1 cut(s) 1306
Csp6I GTAC 7 cut(s) 26, 449, 609, 703, 1563, 2030, 2147
CspAI ACCGGT 2 cut(s) 212, 1736
CspCI CAANNNNNGTGG 2 cut(s) 2193, 2228
CviQI GTAC 7 cut(s) 26, 449, 609, 703, 1563, 2030, 2147
DpnI GATC 5 cut(s) 13, 389, 1704, 1877, 2022
DpnII GATC 5 cut(s) 11, 387, 1702, 1875, 2020
EaeI YGGCCR 1 cut(s) 586
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
Ecl136II GAGCTC 1 cut(s) 1614
Eco130I CCWWGG 5 cut(s) 343, 583, 589, 799, 935
Eco24I GRGCYC 2 cut(s) 1616, 1719
Eco31I GGTCTC 3 cut(s) 85, 1134, 1635
Eco47I GGWCC 5 cut(s) 902, 1436, 1481, 1522, 1944
Eco53kI GAGCTC 1 cut(s) 1614
Eco57I CTGAAG 1 cut(s) 1065
Eco81I CCTNAGG 2 cut(s) 237, 2061
Eco88I CYCGRG 1 cut(s) 1774
EcoICRI GAGCTC 1 cut(s) 1614
EcoO109I RGGNCCY 1 cut(s) 931
EcoRI GAATTC 1 cut(s) 294
EcoRII CCWGG 3 cut(s) 1453, 1505, 2088
EcoT14I CCWWGG 5 cut(s) 343, 583, 589, 799, 935
EcoT22I ATGCAT 1 cut(s) 196
EcoT38I GRGCYC 2 cut(s) 1616, 1719
ErhI CCWWGG 5 cut(s) 343, 583, 589, 799, 935
FaqI GGGAC 2 cut(s) 435, 1021
FauI CCCGC 2 cut(s) 412, 1726
FbaI TGATCA 2 cut(s) 387, 1702
FokI GGATG 5 cut(s) 411, 842, 1481, 2059, 2153
FriOI GRGCYC 2 cut(s) 1616, 1719
FspBI CTAG 8 cut(s) 17, 465, 606, 1431, 1499, 2001, 2199, 2230
GlaI GCGC 2 cut(s) 990, 2131
GsaI CCCAGC 2 cut(s) 720, 1042
GsuI CTGGAG 2 cut(s) 555, 1617
HaeII RGCGCY 1 cut(s) 2133
HaeIII GGCC 6 cut(s) 588, 656, 932, 1510, 1746, 1995
HapII CCGG 9 cut(s) 77, 213, 287, 408, 461, 1485, 1737, 1775, 1997
HgaI GACGC 1 cut(s) 1306
HhaI GCGC 2 cut(s) 991, 2132
Hin6I GCGC 2 cut(s) 989, 2130
HinP1I GCGC 2 cut(s) 989, 2130
HincII GTYRAC 2 cut(s) 1575, 1753
HindII GTYRAC 2 cut(s) 1575, 1753
HindIII AAGCTT 1 cut(s) 512
HinfI GANTC 7 cut(s) 809, 940, 1055, 1234, 1277, 1954, 2222
HpaI GTTAAC 1 cut(s) 1753
HpaII CCGG 9 cut(s) 77, 213, 287, 408, 461, 1485, 1737, 1775, 1997
HphI GGTGA 4 cut(s) 389, 749, 1574, 1852
Hpy166II GTNNAC 7 cut(s) 157, 781, 1522, 1575, 1753, 2056, 2086
Hpy188I TCNGA 5 cut(s) 87, 945, 1394, 1464, 2182
Hpy8I GTNNAC 7 cut(s) 157, 781, 1522, 1575, 1753, 2056, 2086
Hpy99I CGWCG 1 cut(s) 1300
HpyAV CCTTC 8 cut(s) 104, 374, 437, 438, 915, 1139, 1679, 1932
HpyCH4III ACNGT 6 cut(s) 154, 737, 949, 1063, 1387, 1981
HpyCH4IV ACGT 7 cut(s) 602, 783, 1140, 1544, 1671, 1989, 2099
HpySE526I ACGT 7 cut(s) 602, 783, 1140, 1544, 1671, 1989, 2099
HspAI GCGC 2 cut(s) 989, 2130
Kpn2I TCCGGA 1 cut(s) 286
KpnI GGTACC 1 cut(s) 1566
Ksp22I TGATCA 2 cut(s) 387, 1702
KspAI GTTAAC 1 cut(s) 1753
KspI CCGCGG 1 cut(s) 1722
Kzo9I GATC 5 cut(s) 11, 387, 1702, 1875, 2020
LmnI GCTCC 6 cut(s) 34, 416, 953, 1293, 1411, 2092
Lsp1109I GCAGC 9 cut(s) 233, 601, 736, 899, 1213, 1300, 1514, 1610, 1624
LweI GCATC 3 cut(s) 748, 1387, 1503
MaeI CTAG 8 cut(s) 17, 465, 606, 1431, 1499, 2001, 2199, 2230
MaeII ACGT 7 cut(s) 602, 783, 1140, 1544, 1671, 1989, 2099
MaeIII GTNAC 2 cut(s) 1180, 2194
MalI GATC 5 cut(s) 13, 389, 1704, 1877, 2022
MbiI CCGCTC 1 cut(s) 419
MboI GATC 5 cut(s) 11, 387, 1702, 1875, 2020
MboII GAAGA 6 cut(s) 184, 782, 1058, 1214, 2015, 2135
MfeI CAATTG 1 cut(s) 840
MflI RGATCY 1 cut(s) 2020
MhlI GDGCHC 6 cut(s) 212, 789, 1290, 1616, 1719, 2070
MlsI TGGCCA 1 cut(s) 588
MluNI TGGCCA 1 cut(s) 588
MlyI GAGTC 1 cut(s) 1948
MmeI TCCRAC 1 cut(s) 931
Mox20I TGGCCA 1 cut(s) 588
Mph1103I ATGCAT 1 cut(s) 196
MroI TCCGGA 1 cut(s) 286
MroXI GAANNNNTTC 1 cut(s) 813
MscI TGGCCA 1 cut(s) 588
MseI TTAA 6 cut(s) 977, 1329, 1548, 1644, 1752, 1935
MslI CAYNNNNRTG 3 cut(s) 588, 1020, 1587
Msp20I TGGCCA 1 cut(s) 588
MspA1I CMGCKG 6 cut(s) 614, 764, 882, 890, 1637, 1721
MspI CCGG 9 cut(s) 77, 213, 287, 408, 461, 1485, 1737, 1775, 1997
MspR9I CCNGG 8 cut(s) 409, 1455, 1485, 1507, 1775, 1776, 1997, 2090
MunI CAATTG 1 cut(s) 840
MvaI CCWGG 3 cut(s) 1455, 1507, 2090
MvnI CGCG 2 cut(s) 181, 1721
NciI CCSGG 5 cut(s) 409, 1485, 1775, 1776, 1997
NcoI CCATGG 3 cut(s) 583, 589, 935
NdeII GATC 5 cut(s) 11, 387, 1702, 1875, 2020
NlaIV GGNNCC 6 cut(s) 211, 637, 681, 933, 1483, 1564
NruI TCGCGA 1 cut(s) 181
NsiI ATGCAT 1 cut(s) 196
NspI RCATGY 1 cut(s) 1304
OliI CACNNNNGTG 1 cut(s) 1587
PagI TCATGA 3 cut(s) 106, 1239, 1656
PaqCI CACCTGC 1 cut(s) 623
PdmI GAANNNNTTC 1 cut(s) 813
PfeI GAWTC 6 cut(s) 809, 940, 1055, 1234, 1277, 2222
PflFI GACNNNGTC 1 cut(s) 1957
PinAI ACCGGT 2 cut(s) 212, 1736
PleI GAGTC 1 cut(s) 1948
PpsI GAGTC 1 cut(s) 1948
Psp124BI GAGCTC 1 cut(s) 1616
Psp1406I AACGTT 3 cut(s) 1140, 1544, 1989
Psp6I CCWGG 3 cut(s) 1453, 1505, 2088
PspFI CCCAGC 2 cut(s) 716, 1038
PspGI CCWGG 3 cut(s) 1453, 1505, 2088
PspN4I GGNNCC 6 cut(s) 211, 637, 681, 933, 1483, 1564
PspPI GGNCC 7 cut(s) 902, 931, 1436, 1481, 1522, 1944, 1994
PstI CTGCAG 2 cut(s) 619, 1318
PsuI RGATCY 1 cut(s) 2020
PsyI GACNNNGTC 1 cut(s) 1957
PvuII CAGCTG 3 cut(s) 882, 890, 1637
RruI TCGCGA 1 cut(s) 181
RsaI GTAC 7 cut(s) 27, 450, 610, 704, 1564, 2031, 2148
RsaNI GTAC 7 cut(s) 26, 449, 609, 703, 1563, 2030, 2147
RseI CAYNNNNRTG 3 cut(s) 588, 1020, 1587
SacI GAGCTC 1 cut(s) 1616
SacII CCGCGG 1 cut(s) 1722
SaqAI TTAA 6 cut(s) 977, 1329, 1548, 1644, 1752, 1935
Sau3AI GATC 5 cut(s) 11, 387, 1702, 1875, 2020
Sau96I GGNCC 7 cut(s) 902, 931, 1436, 1481, 1522, 1944, 1994
SchI GAGTC 1 cut(s) 1948
ScrFI CCNGG 8 cut(s) 409, 1455, 1485, 1507, 1775, 1776, 1997, 2090
SduI GDGCHC 6 cut(s) 212, 789, 1290, 1616, 1719, 2070
SfaNI GCATC 3 cut(s) 748, 1387, 1503
SfcI CTRYAG 3 cut(s) 615, 1314, 1359
Sfr303I CCGCGG 1 cut(s) 1722
SgrBI CCGCGG 1 cut(s) 1722
SinI GGWCC 5 cut(s) 902, 1436, 1481, 1522, 1944
SmaI CCCGGG 1 cut(s) 1776
SmiMI CAYNNNNRTG 3 cut(s) 588, 1020, 1587
SmlI CTYRAG 4 cut(s) 1351, 1607, 1712, 1884
SmoI CTYRAG 4 cut(s) 1351, 1607, 1712, 1884
SpeI ACTAGT 1 cut(s) 2198
SsiI CCGC 7 cut(s) 199, 419, 612, 762, 854, 1719, 1721
SspI AATATT 3 cut(s) 873, 975, 1189
SspMI CTAG 8 cut(s) 17, 465, 606, 1431, 1499, 2001, 2199, 2230
SstI GAGCTC 1 cut(s) 1616
StyD4I CCNGG 8 cut(s) 407, 1453, 1483, 1505, 1773, 1774, 1995, 2088
StyI CCWWGG 5 cut(s) 343, 583, 589, 799, 935
TaaI ACNGT 6 cut(s) 154, 737, 949, 1063, 1387, 1981
TaiI ACGT 7 cut(s) 605, 786, 1143, 1547, 1674, 1992, 2102
TaqI TCGA 4 cut(s) 10, 1053, 1295, 1683
TatI WGTACW 2 cut(s) 25, 702
TauI GCSGC 2 cut(s) 764, 857
TfiI GAWTC 6 cut(s) 809, 940, 1055, 1234, 1277, 2222
Tru1I TTAA 6 cut(s) 977, 1329, 1548, 1644, 1752, 1935
Tru9I TTAA 6 cut(s) 977, 1329, 1548, 1644, 1752, 1935
TscAI CASTG 4 cut(s) 970, 1390, 1878, 1986
TseI GCWGC 9 cut(s) 221, 614, 749, 887, 1226, 1313, 1502, 1598, 1637
TspDTI ATGAA 5 cut(s) 123, 179, 456, 1256, 1914
TspGWI ACGGA 1 cut(s) 366
TspMI CCCGGG 1 cut(s) 1774
TspRI CASTG 4 cut(s) 970, 1390, 1878, 1986
Tth111I GACNNNGTC 1 cut(s) 1957
VpaK11BI GGWCC 5 cut(s) 902, 1436, 1481, 1522, 1944
XapI RAATTY 4 cut(s) 294, 529, 622, 895
XbaI TCTAGA 1 cut(s) 1430
XceI RCATGY 1 cut(s) 1304
XcmI CCANNNNNNNNNTGG 1 cut(s) 1253
XmaI CCCGGG 1 cut(s) 1774
XmnI GAANNNNTTC 1 cut(s) 813
XspI CTAG 8 cut(s) 17, 465, 606, 1431, 1499, 2001, 2199, 2230
Zsp2I ATGCAT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.