Rmu_sc0006398.1_g000008

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006398.1
OG0000302
Physical Location & Seq
Reverse (-)
22376 .. 24840
2465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006398.1_g000008.1.cds

Sequence Viewer

Length: 2376 bp
atggagaacaaaaagtgtgtagtgatgcagatcatataccttcttggctttagttgcatgttttacttgtcactagcagttgctgtcgaaaaagtggcagtactggaaccacaaggtgagacagatagcatgcaaatttatatagttttggttgaaaggccacccagcaatttcaatgcacaatctgatcatgaagatttagagagttggtaccagtcatttctgccccaaactattgcaaactcaaaccagctcatgaagccacgaatggtttatgcataccacaatgtggccacaggatttgcagcaaagctgactggggaggaagttgaagaaatggagaagaaagatgggtttatctacgctcatccagaacggaatctgcaattgcacacaactcatagtcctaacttcttggggctgcaacaaggattgggactttggaaagggtctaattatggtgcaggaatcattattggagtcttggatactggaataacaccaggtcatccctcattcagcgacgaaggagtgccacctcctccggctaaatggaaaggcaagtgtgatttcaatggcacattctgcaacaacaagcttattggtgccagaagtttccaaggtggtaaaactaccggagctccaccagttgaccttgacggccatggaacccacacgtcgagcacagctggaggaaattttggtgggaacactaccggagctccaccagttgacattgcaggccatggaacccacacgtcgagcacagctggtggaaattttgtgaaaggtgccaacgtgtttggaatggcaaatggaacagcctctggcatggcaccttatgctcacttggcaatgtaccaagtttgttctgaacagagttgttctgaaagtgacattctagctgctattgacactgctattgatgatggcgtggatgtgctatccatctcacttggtggtggctcagctactttttattgggattttattgcaattggtgcatttgcagcaattcagaagggaatttttgtcagctgttcggctggaaatgcagggcctgactactctactacatcaaatgaggccccatggattttgacagttggagcaagctccattgacagaaacataagagcaatagcacagcttggaaatgggcaagaatatgacggggaatcagtattccagcctaaagattttagttcaaaacagttacctcttgtttatgcaggtgaccatagccatgattcatcagctctttgcgatgtagggtcccttacaaatgttgaagggaaagtagtggtgtgtgagctaggtggcggaattggaagaattgcaaaaggggtagaagtgaaaagagctggtggtgttgccatgattcttgtcaacccagactttgctggctataccaccatagcagacgttcacgtgcttccggcaacacatgtaagttactctgcgggggtgagtatcaaaacctatataaactcaacctcaactcctacagcaacaatcttgttcaaaggcactgtcattggtgatcagcttgctccgaaggtctctttcttttcatcaagaggaccaagcctcgcaagtcctggcattttgaaacctgacataattggtcccggtgtgagcatcctagctgcatggcctttttcggtggacaatgccacagaatctaaagccacatttaacattatttccggtacttcaatgtcatgccctcacctgactggcattgtagccttactcaagagctcacaccctgactggtcaccagctgccattaagtctgccatcatgacaactgctgaagtaaacaacctcgcaggctctgccataatggatgaaacattatttgcagcagacctctttgccattggtgcaggtcatgttaacccttcaaaagcaaatgaccctgggctcatctacgacacgcaaccagatgactacattccttacatgtgtggtttgaactacacagaccaacagatagcaatagtcacccggaaattagtgaaatgctctgaagtaggagccatacccgaagcacagctaaattatcccacattttctctctttatagatcctgcagggaagcctcagcattacacaagaacagtgacgaatgttggccccgctaaatcaacttatgaagtagccgttgtaagtccacataacatagacatcagtgtgcaacctgaggagctcacattcacagaggttaaccagacaatgacgtatcatgtggtgtttaccgcacaaaaaggtgctgcgaagaatggtataaaattttctcagggatatctgagctgggtctctcaccagcattctgttagaagccaaatatctgtgatctttgacacttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

791

Amino Acids

83.72

Weight (kDa)

5.4

Isoelectric Point (pI)

33.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1223
Acc36I ACCTGC 2 cut(s) 1223, 1883
Acc65I GGTACC 1 cut(s) 210
AccB1I GGYRCC 4 cut(s) 210, 605, 791, 835
AccB7I CCANNNNNTGG 1 cut(s) 289
AciI CCGC 4 cut(s) 1323, 1463, 2145, 2265
AclWI GGATC 1 cut(s) 2087
AcoI YGGCCR 2 cut(s) 291, 661
AcsI RAATTY 5 cut(s) 135, 697, 778, 1026, 2297
AcuI CTGAAG 2 cut(s) 1839, 2055
AcvI CACGTG 1 cut(s) 1432
AdeI CACNNNGTG 3 cut(s) 116, 289, 959
AfaI GTAC 4 cut(s) 102, 212, 860, 1714
AfiI CCNNNNNNNGG 1 cut(s) 289
AflIII ACRYGT 5 cut(s) 675, 756, 798, 1447, 1968
AjiI CACGTC 2 cut(s) 678, 759
AjnI CCWGG 3 cut(s) 502, 1600, 1924
Alw21I GWGCWC 6 cut(s) 643, 686, 724, 767, 1766, 2217
Alw26I GTCTC 3 cut(s) 113, 1567, 2329
AlwI GGATC 1 cut(s) 2087
AlwNI CAGNNNCTG 4 cut(s) 83, 827, 1061, 1841
AoxI GGCC 8 cut(s) 158, 291, 661, 742, 1058, 1086, 1655, 2140
ApeKI GCWGC 8 cut(s) 305, 421, 905, 1010, 1649, 1787, 1868, 2279
ApoI RAATTY 5 cut(s) 135, 697, 778, 1026, 2297
ArsI GACNNNNNNTTYG 2 cut(s) 1518, 1550
Asp718I GGTACC 1 cut(s) 210
AspS9I GGNCC 6 cut(s) 1058, 1087, 1275, 1583, 1628, 2141
AsuC2I CCSGG 2 cut(s) 1632, 2014
AsuHPI GGTGA 8 cut(s) 128, 1247, 1480, 1553, 1724, 1773, 2002, 2321
AvaII GGWCC 3 cut(s) 1275, 1583, 1628
AxyI CCTNAGG 1 cut(s) 2208
BalI TGGCCA 1 cut(s) 293
BanI GGYRCC 4 cut(s) 210, 605, 791, 835
BanII GRGCYC 5 cut(s) 643, 724, 1766, 1932, 2217
BbrPI CACGTG 1 cut(s) 1432
Bbv12I GWGCWC 6 cut(s) 643, 686, 724, 767, 1766, 2217
BbvCI CCTCAGC 1 cut(s) 2109
BbvI GCAGC 8 cut(s) 317, 408, 892, 1022, 1636, 1774, 1880, 2266
BccI CCATC 4 cut(s) 344, 923, 956, 1811
BceAI ACGGC 2 cut(s) 676, 2153
BciT130I CCWGG 3 cut(s) 504, 1602, 1926
BciVI GTATCC 1 cut(s) 481
BclI TGATCA 2 cut(s) 187, 1543
BcnI CCSGG 2 cut(s) 1632, 2014
BcoDI GTCTC 3 cut(s) 113, 1567, 2329
BfaI CTAG 4 cut(s) 74, 902, 1316, 1646
BfmI CTRYAG 2 cut(s) 1506, 2097
BfuAI ACCTGC 2 cut(s) 1223, 1883
BfuI GTATCC 1 cut(s) 481
BisI GCNGC 8 cut(s) 306, 422, 906, 1011, 1650, 1788, 1869, 2280
BlpI GCTNAGC 1 cut(s) 967
BlsI GCNGC 8 cut(s) 307, 423, 907, 1012, 1651, 1789, 1870, 2281
BmcAI AGTACT 1 cut(s) 102
Bme1390I CCNGG 5 cut(s) 504, 1602, 1632, 1926, 2014
Bme18I GGWCC 3 cut(s) 1275, 1583, 1628
BmgBI CACGTC 2 cut(s) 678, 759
BmgT120I GGNCC 6 cut(s) 1058, 1087, 1275, 1583, 1628, 2141
BmrFI CCNGG 5 cut(s) 504, 1602, 1632, 1926, 2014
BmrI ACTGGG 1 cut(s) 327
BmsI GCATC 2 cut(s) 15, 1650
BmuI ACTGGG 1 cut(s) 327
BpmI CTGGAG 1 cut(s) 711
Bpu10I CCTNAGC 1 cut(s) 2109
Bpu1102I GCTNAGC 1 cut(s) 967
BpuEI CTTGAG 1 cut(s) 1742
BpuMI CCSGG 2 cut(s) 1632, 2014
BsaAI YACGTR 1 cut(s) 1432
BsaBI GATNNNNATC 1 cut(s) 29
BsaI GGTCTC 2 cut(s) 1567, 2329
BsaJI CCNNGG 6 cut(s) 619, 664, 745, 1091, 1924, 1925
BsaWI WCCGGW 3 cut(s) 635, 716, 1709
BsaXI ACNNNNNCTCC 6 cut(s) 625, 655, 706, 736, 1486, 1516
Bsc4I CCNNNNNNNGG 1 cut(s) 289
Bse1I ACTGG 8 cut(s) 108, 214, 322, 496, 647, 728, 1744, 1781
Bse21I CCTNAGG 1 cut(s) 2208
Bse3DI GCAATG 2 cut(s) 735, 861
Bse8I GATNNNNATC 1 cut(s) 29
BseBI CCWGG 3 cut(s) 504, 1602, 1926
BseDI CCNNGG 6 cut(s) 619, 664, 745, 1091, 1924, 1925
BseGI GGATG 5 cut(s) 367, 508, 943, 1641, 1858
BseJI GATNNNNATC 1 cut(s) 29
BseLI CCNNNNNNNGG 1 cut(s) 289
BseMI GCAATG 2 cut(s) 735, 861
BseMII CTCAG 5 cut(s) 981, 2123, 2199, 2306, 2318
BseNI ACTGG 8 cut(s) 108, 214, 322, 496, 647, 728, 1744, 1781
BseRI GAGGAG 2 cut(s) 531, 2225
BseXI GCAGC 8 cut(s) 317, 408, 892, 1022, 1636, 1774, 1880, 2266
BseYI CCCAGC 2 cut(s) 164, 2319
BsgI GTGCAG 2 cut(s) 483, 1911
BshFI GGCC 8 cut(s) 160, 293, 663, 744, 1060, 1088, 1657, 2142
BshNI GGYRCC 4 cut(s) 210, 605, 791, 835
BsiHKAI GWGCWC 6 cut(s) 643, 686, 724, 767, 1766, 2217
BsiSI CCGG 7 cut(s) 545, 636, 717, 1439, 1632, 1710, 2014
BslFI GGGAC 3 cut(s) 450, 1261, 1614
BslI CCNNNNNNNGG 1 cut(s) 289
BsmAI GTCTC 3 cut(s) 113, 1567, 2329
BsmFI GGGAC 3 cut(s) 450, 1261, 1614
BsmI GAATGC 1 cut(s) 2335
BsnI GGCC 8 cut(s) 160, 293, 663, 744, 1060, 1088, 1657, 2142
Bso31I GGTCTC 2 cut(s) 1567, 2329
Bsp1286I GDGCHC 7 cut(s) 643, 686, 724, 767, 1766, 1932, 2217
Bsp143I GATC 5 cut(s) 30, 187, 1543, 2092, 2361
Bsp1720I GCTNAGC 1 cut(s) 967
Bsp19I CCATGG 3 cut(s) 664, 745, 1091
BspACI CCGC 4 cut(s) 1323, 1463, 2145, 2265
BspANI GGCC 8 cut(s) 160, 293, 663, 744, 1060, 1088, 1657, 2142
BspCNI CTCAG 5 cut(s) 980, 2122, 2200, 2307, 2317
BspHI TCATGA 3 cut(s) 190, 255, 1806
BspMAI CTGCAG 1 cut(s) 2101
BspMI ACCTGC 2 cut(s) 1223, 1883
BspPI GGATC 1 cut(s) 2087
BspT107I GGYRCC 4 cut(s) 210, 605, 791, 835
BspTNI GGTCTC 2 cut(s) 1567, 2329
BsrDI GCAATG 2 cut(s) 735, 861
BsrI ACTGG 8 cut(s) 108, 214, 322, 496, 647, 728, 1744, 1781
BssECI CCNNGG 6 cut(s) 619, 664, 745, 1091, 1924, 1925
BssMI GATC 5 cut(s) 30, 187, 1543, 2092, 2361
BssT1I CCWWGG 4 cut(s) 619, 664, 745, 1091
Bst2UI CCWGG 3 cut(s) 504, 1602, 1926
Bst4CI ACNGT 4 cut(s) 1105, 1215, 1534, 2128
BstAPI GCANNNNNTGC 4 cut(s) 585, 842, 1001, 1841
BstBAI YACGTR 1 cut(s) 1432
BstC8I GCNNGC 6 cut(s) 131, 742, 1114, 1405, 1551, 1837
BstDEI CTNAG 5 cut(s) 967, 2109, 2208, 2304, 2315
BstDSI CCRYGG 3 cut(s) 664, 745, 1091
BstEII GGTNACC 2 cut(s) 1235, 1779
BstF5I GGATG 5 cut(s) 367, 508, 943, 1641, 1858
BstKTI GATC 5 cut(s) 33, 190, 1546, 2095, 2364
BstMAI GTCTC 3 cut(s) 113, 1567, 2329
BstMBI GATC 5 cut(s) 30, 187, 1543, 2092, 2361
BstNI CCWGG 3 cut(s) 504, 1602, 1926
BstNSI RCATGY 4 cut(s) 61, 133, 1451, 1972
BstPI GGTNACC 2 cut(s) 1235, 1779
BstSCI CCNGG 5 cut(s) 502, 1600, 1630, 1924, 2012
BstSFI CTRYAG 2 cut(s) 1506, 2097
BstV1I GCAGC 8 cut(s) 317, 408, 892, 1022, 1636, 1774, 1880, 2266
BstX2I RGATCY 1 cut(s) 2092
BstYI RGATCY 1 cut(s) 2092
Bsu36I CCTNAGG 1 cut(s) 2208
BsuI GTATCC 1 cut(s) 481
BsuRI GGCC 8 cut(s) 160, 293, 663, 744, 1060, 1088, 1657, 2142
BtgI CCRYGG 3 cut(s) 664, 745, 1091
BtgZI GCGATG 1 cut(s) 1281
BtrI CACGTC 2 cut(s) 678, 759
BtsCI GGATG 5 cut(s) 367, 508, 943, 1641, 1858
BtsI GCAGTG 1 cut(s) 915
BtsIMutI CAGTG 4 cut(s) 915, 1530, 2133, 2203
BveI ACCTGC 2 cut(s) 1223, 1883
Cac8I GCNNGC 6 cut(s) 131, 742, 1114, 1405, 1551, 1837
CaiI CAGNNNCTG 4 cut(s) 83, 827, 1061, 1841
CciI TCATGA 3 cut(s) 190, 255, 1806
Cfr13I GGNCC 6 cut(s) 1058, 1087, 1275, 1583, 1628, 2141
CsiI ACCWGGT 1 cut(s) 502
Csp6I GTAC 4 cut(s) 101, 211, 859, 1713
CspCI CAANNNNNGTGG 2 cut(s) 283, 318
CviQI GTAC 4 cut(s) 101, 211, 859, 1713
DdeI CTNAG 5 cut(s) 967, 2109, 2208, 2304, 2315
DpnI GATC 5 cut(s) 32, 189, 1545, 2094, 2363
DpnII GATC 5 cut(s) 30, 187, 1543, 2092, 2361
DraIII CACNNNGTG 3 cut(s) 116, 289, 959
EaeI YGGCCR 2 cut(s) 291, 661
EciI GGCGGA 1 cut(s) 1338
Ecl136II GAGCTC 4 cut(s) 641, 722, 1764, 2215
Eco130I CCWWGG 4 cut(s) 619, 664, 745, 1091
Eco24I GRGCYC 5 cut(s) 643, 724, 1766, 1932, 2217
Eco31I GGTCTC 2 cut(s) 1567, 2329
Eco32I GATATC 1 cut(s) 2312
Eco47I GGWCC 3 cut(s) 1275, 1583, 1628
Eco53kI GAGCTC 4 cut(s) 641, 722, 1764, 2215
Eco57I CTGAAG 2 cut(s) 1839, 2055
Eco72I CACGTG 1 cut(s) 1432
Eco81I CCTNAGG 1 cut(s) 2208
Eco91I GGTNACC 2 cut(s) 1235, 1779
EcoICRI GAGCTC 4 cut(s) 641, 722, 1764, 2215
EcoO109I RGGNCCY 3 cut(s) 1058, 1087, 1275
EcoO65I GGTNACC 2 cut(s) 1235, 1779
EcoRII CCWGG 3 cut(s) 502, 1600, 1924
EcoRV GATATC 1 cut(s) 2312
EcoT14I CCWWGG 4 cut(s) 619, 664, 745, 1091
EcoT22I ATGCAT 1 cut(s) 280
EcoT38I GRGCYC 5 cut(s) 643, 724, 1766, 1932, 2217
ErhI CCWWGG 4 cut(s) 619, 664, 745, 1091
FaqI GGGAC 3 cut(s) 450, 1261, 1614
FauI CCCGC 2 cut(s) 1456, 2152
FbaI TGATCA 2 cut(s) 187, 1543
Fnu4HI GCNGC 8 cut(s) 306, 422, 906, 1011, 1650, 1788, 1869, 2280
FokI GGATG 5 cut(s) 354, 495, 950, 1628, 1865
FriOI GRGCYC 5 cut(s) 643, 724, 1766, 1932, 2217
Fsp4HI GCNGC 8 cut(s) 306, 422, 906, 1011, 1650, 1788, 1869, 2280
FspBI CTAG 4 cut(s) 74, 902, 1316, 1646
GluI GCNGC 8 cut(s) 306, 422, 906, 1011, 1650, 1788, 1869, 2280
GsaI CCCAGC 2 cut(s) 168, 2323
GsuI CTGGAG 1 cut(s) 711
HaeIII GGCC 8 cut(s) 160, 293, 663, 744, 1060, 1088, 1657, 2142
HapII CCGG 7 cut(s) 545, 636, 717, 1439, 1632, 1710, 2014
HincII GTYRAC 5 cut(s) 652, 733, 1390, 1903, 2233
HindII GTYRAC 5 cut(s) 652, 733, 1390, 1903, 2233
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 7 cut(s) 379, 468, 480, 1178, 1250, 1381, 1682
HpaI GTTAAC 2 cut(s) 1903, 2233
HpaII CCGG 7 cut(s) 545, 636, 717, 1439, 1632, 1710, 2014
HphI GGTGA 8 cut(s) 128, 1247, 1480, 1553, 1724, 1773, 2002, 2321
Hpy188I TCNGA 7 cut(s) 187, 874, 889, 1020, 1557, 2035, 2316
Hpy188III TCNNGA 6 cut(s) 191, 256, 371, 1578, 1759, 1807
Hpy99I CGWCG 3 cut(s) 527, 682, 763
HpyAV CCTTC 6 cut(s) 50, 521, 1015, 1286, 1552, 1917
HpyCH4III ACNGT 4 cut(s) 1105, 1215, 1534, 2128
HpyCH4IV ACGT 6 cut(s) 677, 758, 798, 1425, 1431, 2246
HpyF3I CTNAG 5 cut(s) 967, 2109, 2208, 2304, 2315
HpySE526I ACGT 6 cut(s) 677, 758, 798, 1425, 1431, 2246
KflI GGGWCCC 1 cut(s) 1275
KpnI GGTACC 1 cut(s) 214
Ksp22I TGATCA 2 cut(s) 187, 1543
KspAI GTTAAC 2 cut(s) 1903, 2233
Kzo9I GATC 5 cut(s) 30, 187, 1543, 2092, 2361
LmnI GCTCC 9 cut(s) 638, 646, 719, 727, 1109, 1121, 1558, 2042, 2212
Lsp1109I GCAGC 8 cut(s) 317, 408, 892, 1022, 1636, 1774, 1880, 2266
LweI GCATC 2 cut(s) 15, 1650
MabI ACCWGGT 1 cut(s) 502
MaeI CTAG 4 cut(s) 74, 902, 1316, 1646
MaeII ACGT 6 cut(s) 677, 758, 798, 1425, 1431, 2246
MaeIII GTNAC 8 cut(s) 69, 893, 1215, 1235, 1454, 1779, 2008, 2128
MalI GATC 5 cut(s) 32, 189, 1545, 2094, 2363
MboI GATC 5 cut(s) 30, 187, 1543, 2092, 2361
MboII GAAGA 5 cut(s) 206, 344, 355, 1344, 2296
MfeI CAATTG 2 cut(s) 386, 996
MflI RGATCY 1 cut(s) 2092
MhlI GDGCHC 7 cut(s) 643, 686, 724, 767, 1766, 1932, 2217
MlsI TGGCCA 1 cut(s) 293
MluNI TGGCCA 1 cut(s) 293
MlyI GAGTC 1 cut(s) 489
MmeI TCCRAC 1 cut(s) 1087
Mox20I TGGCCA 1 cut(s) 293
Mph1103I ATGCAT 1 cut(s) 280
MscI TGGCCA 1 cut(s) 293
MseI TTAA 5 cut(s) 1698, 1794, 1902, 2232, 2374
MslI CAYNNNNRTG 2 cut(s) 1245, 2198
Msp20I TGGCCA 1 cut(s) 293
MspA1I CMGCKG 4 cut(s) 689, 770, 1038, 1787
MspI CCGG 7 cut(s) 545, 636, 717, 1439, 1632, 1710, 2014
MspR9I CCNGG 5 cut(s) 504, 1602, 1632, 1926, 2014
MunI CAATTG 2 cut(s) 386, 996
Mva1269I GAATGC 1 cut(s) 2335
MvaI CCWGG 3 cut(s) 504, 1602, 1926
NciI CCSGG 2 cut(s) 1632, 2014
NcoI CCATGG 3 cut(s) 664, 745, 1091
NdeII GATC 5 cut(s) 30, 187, 1543, 2092, 2361
NmuCI GTSAC 6 cut(s) 69, 893, 1235, 1779, 2008, 2128
NsiI ATGCAT 1 cut(s) 280
NspI RCATGY 4 cut(s) 61, 133, 1451, 1972
PaeI GCATGC 1 cut(s) 133
PagI TCATGA 3 cut(s) 190, 255, 1806
PaqCI CACCTGC 1 cut(s) 1223
PasI CCCWGGG 1 cut(s) 1925
PciI ACATGT 2 cut(s) 1447, 1968
PctI GAATGC 1 cut(s) 2335
PfeI GAWTC 6 cut(s) 379, 468, 1178, 1250, 1381, 1682
PflMI CCANNNNNTGG 1 cut(s) 289
PkrI GCNGC 8 cut(s) 307, 423, 907, 1012, 1651, 1789, 1870, 2281
PleI GAGTC 1 cut(s) 488
PmaCI CACGTG 1 cut(s) 1432
PmlI CACGTG 1 cut(s) 1432
PpsI GAGTC 1 cut(s) 488
Ppu21I YACGTR 1 cut(s) 1432
PpuMI RGGWCCY 1 cut(s) 1275
PscI ACATGT 2 cut(s) 1447, 1968
Psp124BI GAGCTC 4 cut(s) 643, 724, 1766, 2217
Psp5II RGGWCCY 1 cut(s) 1275
Psp6I CCWGG 3 cut(s) 502, 1600, 1924
PspCI CACGTG 1 cut(s) 1432
PspEI GGTNACC 2 cut(s) 1235, 1779
PspFI CCCAGC 2 cut(s) 164, 2319
PspGI CCWGG 3 cut(s) 502, 1600, 1924
PspPI GGNCC 6 cut(s) 1058, 1087, 1275, 1583, 1628, 2141
PspPPI RGGWCCY 1 cut(s) 1275
PstI CTGCAG 1 cut(s) 2101
PstNI CAGNNNCTG 4 cut(s) 83, 827, 1061, 1841
PsuI RGATCY 1 cut(s) 2092
PvuII CAGCTG 4 cut(s) 689, 770, 1038, 1787
RsaI GTAC 4 cut(s) 102, 212, 860, 1714
RsaNI GTAC 4 cut(s) 101, 211, 859, 1713
RseI CAYNNNNRTG 2 cut(s) 1245, 2198
SacI GAGCTC 4 cut(s) 643, 724, 1766, 2217
SaqAI TTAA 5 cut(s) 1698, 1794, 1902, 2232, 2374
SatI GCNGC 8 cut(s) 306, 422, 906, 1011, 1650, 1788, 1869, 2280
Sau3AI GATC 5 cut(s) 30, 187, 1543, 2092, 2361
Sau96I GGNCC 6 cut(s) 1058, 1087, 1275, 1583, 1628, 2141
SbfI CCTGCAGG 1 cut(s) 2101
ScaI AGTACT 1 cut(s) 102
SchI GAGTC 1 cut(s) 489
ScrFI CCNGG 5 cut(s) 504, 1602, 1632, 1926, 2014
SdaI CCTGCAGG 1 cut(s) 2101
SduI GDGCHC 7 cut(s) 643, 686, 724, 767, 1766, 1932, 2217
SexAI ACCWGGT 1 cut(s) 502
SfaNI GCATC 2 cut(s) 15, 1650
SfcI CTRYAG 2 cut(s) 1506, 2097
SinI GGWCC 3 cut(s) 1275, 1583, 1628
SmiMI CAYNNNNRTG 2 cut(s) 1245, 2198
SmlI CTYRAG 1 cut(s) 1757
SmoI CTYRAG 1 cut(s) 1757
SphI GCATGC 1 cut(s) 133
Sse8387I CCTGCAGG 1 cut(s) 2101
SsiI CCGC 4 cut(s) 1323, 1463, 2145, 2265
SspMI CTAG 4 cut(s) 74, 902, 1316, 1646
SstI GAGCTC 4 cut(s) 643, 724, 1766, 2217
StyD4I CCNGG 5 cut(s) 502, 1600, 1630, 1924, 2012
StyI CCWWGG 4 cut(s) 619, 664, 745, 1091
TaaI ACNGT 4 cut(s) 1105, 1215, 1534, 2128
TaiI ACGT 6 cut(s) 680, 761, 801, 1428, 1434, 2249
TaqI TCGA 3 cut(s) 87, 680, 761
TatI WGTACW 1 cut(s) 100
TfiI GAWTC 6 cut(s) 379, 468, 1178, 1250, 1381, 1682
Tru1I TTAA 5 cut(s) 1698, 1794, 1902, 2232, 2374
Tru9I TTAA 5 cut(s) 1698, 1794, 1902, 2232, 2374
TscAI CASTG 4 cut(s) 922, 1537, 2133, 2203
TseFI GTSAC 6 cut(s) 69, 893, 1235, 1779, 2008, 2128
TseI GCWGC 8 cut(s) 305, 421, 905, 1010, 1649, 1787, 1868, 2279
Tsp45I GTSAC 6 cut(s) 69, 893, 1235, 1779, 2008, 2128
TspDTI ATGAA 6 cut(s) 207, 272, 1242, 1563, 1869, 2175
TspGWI ACGGA 1 cut(s) 391
TspRI CASTG 4 cut(s) 922, 1537, 2133, 2203
Van91I CCANNNNNTGG 1 cut(s) 289
VpaK11BI GGWCC 3 cut(s) 1275, 1583, 1628
XapI RAATTY 5 cut(s) 135, 697, 778, 1026, 2297
XceI RCATGY 4 cut(s) 61, 133, 1451, 1972
XspI CTAG 4 cut(s) 74, 902, 1316, 1646
ZrmI AGTACT 1 cut(s) 102
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.