pycom04g06430

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000302
Physical Location & Seq
Reverse (-)
6492427 .. 6494685
2259 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g06430.1

Sequence Viewer

Length: 2259 bp
ATGGCTGTGATGCCTTTTCTTACTCTCATTTTCATCCTTAGTTTCTGTTATGTTACCGCTCAGAAAAACGAGCTCTCACCAGCAACTACTAAAACGAGCAATTTACAGACATACGTTGTCCATGTAAGGCAACCAGAGGGCAGAGTTTTTGCTCAAACAGAAGACCTCAAGAGCTGGCACGAATCTTTTCTGCCCGTTACCACAGCAAGCGCAGATGAACCGCCACGCCTGCTTTACTCGTACGAAGAAGTGATCAGTGGTTTTGCTGCTAGACTAACTCAGGACGAAGTAAAGGCAATGCAGGAGATGGATGGCTTTGTGGCAGCATATCCTCAAAGAGTATTCCGTCGGAAAACTACACGCACACCCAACTTCTTGGGGCTGCACCAACAAACCGGAATATGGAAGGATTCAAATTTTGGGAAGGGCGTGATCATTGGCGTGCTGGATGGTGGAATTGAGCCCAACCATCCTTCATTTAGTGGTGCAGGAATTCCACCTCCACCCGCTAAATGGAAAGGGAGGTGTGACTTTAACGCATCAGACTGCAACAACAAATTAATTGGTGCAAGGGCTTTCAATCTTGCAGCTATGGCGTTGAAAGGCGAGAAGCCTGAGGCACCGATCGATATAGACGGACATGGGACCCACACAGCAAGCACTGCTGCTGGTGCATTTGTGCAGAATGCTGATGTTTTAGGGAATGCCAAAGGTACGGCAGTCGGGATCGCACCTCATGCCCACCTAGCAATATACAAGGTGTGCTTCGGAGACCCCTGCCCAGATGCTGATATATTAGCTGCACTTGAAGCAGCTGTGCAGGATGGTGTTGATGTGATCTCAATCTCCCTTGGTGACGTGTCAGTTCCATTTTTCCAAGACACCACTGCAATAGGCTCATTTGCAGCAATCCAAAAAGGAATATTTGTAAGTTGTTCAGCTGGAAATTCTGGCCCATTTAACGGCAAACTATCTAATGAAGCCCCCTGGATGCTAACAGTTGGAGCAAGCACAATAGACAGACGTGTTGTAGCCACAGCAAAACTTGGAAATGGAGAAGTATTTGATGGTGAATCTCTCTTTCAGCCCAGTGATTTCCCTTCAACATTAATGCCTCTTATTTATGCTGGAGTAAATGGTAATGATTCGGCATTGTGTGCTGAAGGATCCTTGAAAGGTCTCCCTGTTAAAGGTAAGGTAGTTGTGTGTGAGCGAGGGGGAGGTATAGGAAGGATTGCTAAGGGAGAAGAAGTGAAAAACGCTGGAGGTGCTGCCATGATTCTTTTGAATGAAGAAACTGACGGCGTCAGTACTTTAGCTGAAGTTCATGTTCTTCCGGCAACACATGTGAGCTACGCAGCTGGACTGAAGATCAAAGCATATATAAATTCAACAGTAACACCAACAGCAACAATCTTATTCAAAGGAACTGTCATCGGAGATTCATCTACTCCAGTGGTTGCTTCCTTCTCTTCAAGAGGCCCCAGCCTGGCAAGTCCAGGTATTCTGAAACCAGACATTATTGGCCCTGGAGTAAGCATTCTTGCTGCATCGCCATTTCCCGTTGACAATACCACAAAATCAAAAGTCAATTTCAAAATAATGTCTGGAACTTCAATGTCTTGCCCGCATCTCAGTGGAATTGCAGCTTTGCTTAAGAGCTCACACCCTTACTGGTCACCAGCTGCCATTAAATCTGCTATAATGACTTCTGATGATCTTCTAAATTTAGAAGGCAAACCTATTCTCGATGAACAACTTCAGCCTGCAGATGTTTTAGCCACAGGTGCCGGCCAGGTCAATCCATCAAAGGCAAATGACCCGGGATTAATTTATGATATTCAACCTGATGATTATATCCCTTATCTTTGTGGCTTGGGCTATAAAGATGATGAAATCAGTATCATTGTGCATAGACAAATAAAATGCTCAATGGTATCGAGCATACCTGAAGGAGAGCTGAACTACCCTTCGTTTTCTGTCACACTGGGACCATCTCAGACATTCACACGAACTCTGACAAATGTTGGTGAGGCATATTCATCTTATGCAGTCAAGGTTAATGCACCAGAAGGGGTATATGTGAGTGTCAAGCCCAAAACACTTAACTTCACAAAGGTGAACCAGAAGATGTCGTATTCAGTGACATTTAGCCGTATTGGTTCAAAAGGTGAAGCAGGTGAATTCACTAAAGGATTCTTAACATGGGTTTCTGCTAAATATGTTGTTAGGAGTCCGGTTTCTGTTAAATTACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

753

Amino Acids

79.54

Weight (kDa)

6.26

Isoelectric Point (pI)

31.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 37 - 114 1.2e-11 Peptidase inhibitor I9
Peptidase_S8 PF00082 141 - 572 6.4e-45 Subtilase family
PA PF02225 372 - 458 2e-12 PA domain
fn3_6 PF17766 653 - 749 4e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2168
Acc36I ACCTGC 1 cut(s) 2168
AccB1I GGYRCC 2 cut(s) 619, 1787
AccBSI CCGCTC 1 cut(s) 59
AciI CCGC 4 cut(s) 57, 221, 507, 1628
AclWI GGATC 3 cut(s) 734, 1161, 1174
AcoI YGGCCR 1 cut(s) 1792
AcsI RAATTY 6 cut(s) 415, 492, 946, 1387, 1726, 2183
AcuI CTGAAG 5 cut(s) 1182, 1341, 1388, 1745, 1971
AcyI GRCGYC 1 cut(s) 1305
AfaI GTAC 3 cut(s) 242, 715, 1312
AfiI CCNNNNNNNGG 5 cut(s) 402, 513, 962, 1190, 1489
AflII CTTAAG 1 cut(s) 1655
AflIII ACRYGT 3 cut(s) 858, 1024, 1345
AjiI CACGTC 2 cut(s) 859, 1025
AjnI CCWGG 5 cut(s) 986, 1488, 1498, 1528, 1794
AleI CACNNNNGTG 1 cut(s) 2117
Alw21I GWGCWC 2 cut(s) 75, 1664
Alw26I GTCTC 2 cut(s) 765, 1184
AlwI GGATC 3 cut(s) 734, 1161, 1174
AlwNI CAGNNNCTG 1 cut(s) 788
Ama87I CYCGRG 1 cut(s) 1822
AoxI GGCC 4 cut(s) 952, 1480, 1525, 1792
ApoI RAATTY 6 cut(s) 415, 492, 946, 1387, 1726, 2183
ArsI GACNNNNNNTTYG 2 cut(s) 1416, 1448
AseI ATTAAT 3 cut(s) 560, 1109, 1829
Asp700I GAANNNNTTC 2 cut(s) 186, 1758
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 5 cut(s) 645, 953, 1481, 1526, 1991
AsuC2I CCSGG 2 cut(s) 1823, 1824
AsuHPI GGTGA 8 cut(s) 69, 866, 1082, 1671, 2042, 2131, 2183, 2192
AvaI CYCGRG 1 cut(s) 1822
AvaII GGWCC 2 cut(s) 645, 1991
AxyI CCTNAGG 1 cut(s) 615
BaeI ACNNNNGTAYC 2 cut(s) 705, 738
BamHI GGATCC 1 cut(s) 1166
BanI GGYRCC 2 cut(s) 619, 1787
BanII GRGCYC 3 cut(s) 75, 465, 1664
BbsI GAAGAC 1 cut(s) 168
Bbv12I GWGCWC 2 cut(s) 75, 1664
BccI CCATC 8 cut(s) 301, 305, 443, 477, 818, 1061, 1813, 2002
BceAI ACGGC 4 cut(s) 732, 979, 1318, 2139
BciT130I CCWGG 5 cut(s) 988, 1490, 1500, 1530, 1796
BclI TGATCA 2 cut(s) 252, 432
BcnI CCSGG 2 cut(s) 1823, 1824
BcoDI GTCTC 2 cut(s) 765, 1184
BfaI CTAG 2 cut(s) 270, 746
BfmI CTRYAG 1 cut(s) 1767
BfrI CTTAAG 1 cut(s) 1655
BfuAI ACCTGC 1 cut(s) 2168
BmcAI AGTACT 1 cut(s) 1312
Bme1390I CCNGG 7 cut(s) 988, 1490, 1500, 1530, 1796, 1823, 1824
Bme18I GGWCC 2 cut(s) 645, 1991
BmeT110I CYCGRG 1 cut(s) 1822
BmgBI CACGTC 2 cut(s) 859, 1025
BmgT120I GGNCC 5 cut(s) 645, 953, 1481, 1526, 1991
BmiI GGNNCC 7 cut(s) 621, 646, 647, 1168, 1483, 1789, 1992
BmrFI CCNGG 7 cut(s) 988, 1490, 1500, 1530, 1796, 1823, 1824
BmrI ACTGGG 2 cut(s) 1083, 1997
BmsI GCATC 5 cut(s) 548, 775, 981, 1559, 1639
BmuI ACTGGG 2 cut(s) 1083, 1997
BpiI GAAGAC 1 cut(s) 168
BpmI CTGGAG 4 cut(s) 1149, 1284, 1437, 1551
Bpu10I CCTNAGC 1 cut(s) 1239
BpuEI CTTGAG 1 cut(s) 152
BpuMI CCSGG 2 cut(s) 1823, 1824
Bsa29I ATCGAT 1 cut(s) 627
BsaBI GATNNNNATC 1 cut(s) 842
BsaHI GRCGYC 1 cut(s) 1305
BsaI GGTCTC 2 cut(s) 765, 1184
BsaJI CCNNGG 4 cut(s) 850, 986, 1528, 1822
BsaWI WCCGGW 2 cut(s) 395, 2236
BsaXI ACNNNNNCTCC 2 cut(s) 1236, 1266
Bsc4I CCNNNNNNNGG 5 cut(s) 402, 513, 962, 1190, 1489
Bse118I RCCGGY 1 cut(s) 1790
Bse1I ACTGG 4 cut(s) 1089, 1454, 1679, 1992
Bse21I CCTNAGG 1 cut(s) 615
Bse3DI GCAATG 1 cut(s) 303
Bse8I GATNNNNATC 1 cut(s) 842
BseBI CCWGG 5 cut(s) 988, 1490, 1500, 1530, 1796
BseCI ATCGAT 1 cut(s) 627
BseDI CCNNGG 4 cut(s) 850, 986, 1528, 1822
BseGI GGATG 6 cut(s) 33, 316, 454, 469, 829, 996
BseJI GATNNNNATC 1 cut(s) 842
BseLI CCNNNNNNNGG 5 cut(s) 402, 513, 962, 1190, 1489
BseMI GCAATG 1 cut(s) 303
BseMII CTCAG 5 cut(s) 74, 293, 606, 1648, 2012
BseNI ACTGG 4 cut(s) 1089, 1454, 1679, 1992
BseYI CCCAGC 1 cut(s) 1484
BsgI GTGCAG 5 cut(s) 368, 507, 701, 786, 839
Bsh1285I CGRYCG 1 cut(s) 627
BshFI GGCC 4 cut(s) 954, 1482, 1527, 1794
BshNI GGYRCC 2 cut(s) 619, 1787
BshVI ATCGAT 1 cut(s) 627
BsiEI CGRYCG 1 cut(s) 627
BsiHKAI GWGCWC 2 cut(s) 75, 1664
BsiHKCI CYCGRG 1 cut(s) 1822
BsiSI CCGG 5 cut(s) 396, 1337, 1791, 1823, 2237
BsiWI CGTACG 1 cut(s) 240
BslFI GGGAC 2 cut(s) 658, 2004
BslI CCNNNNNNNGG 5 cut(s) 402, 513, 962, 1190, 1489
BsmAI GTCTC 2 cut(s) 765, 1184
BsmFI GGGAC 2 cut(s) 658, 2004
BsmI GAATGC 3 cut(s) 691, 709, 1539
BsnI GGCC 4 cut(s) 954, 1482, 1527, 1794
Bso31I GGTCTC 2 cut(s) 765, 1184
BsoBI CYCGRG 1 cut(s) 1822
Bsp1286I GDGCHC 3 cut(s) 75, 465, 1664
Bsp143I GATC 8 cut(s) 252, 432, 624, 726, 837, 1166, 1371, 1717
BspACI CCGC 4 cut(s) 57, 221, 507, 1628
BspANI GGCC 4 cut(s) 954, 1482, 1527, 1794
BspCNI CTCAG 5 cut(s) 73, 292, 607, 1647, 2011
BspDI ATCGAT 1 cut(s) 627
BspLI GGNNCC 7 cut(s) 621, 646, 647, 1168, 1483, 1789, 1992
BspMAI CTGCAG 1 cut(s) 1771
BspMI ACCTGC 1 cut(s) 2168
BspPI GGATC 3 cut(s) 734, 1161, 1174
BspT107I GGYRCC 2 cut(s) 619, 1787
BspTI CTTAAG 1 cut(s) 1655
BspTNI GGTCTC 2 cut(s) 765, 1184
BsrBI CCGCTC 1 cut(s) 59
BsrDI GCAATG 1 cut(s) 303
BsrFI RCCGGY 1 cut(s) 1790
BsrI ACTGG 4 cut(s) 1089, 1454, 1679, 1992
BssAI RCCGGY 1 cut(s) 1790
BssECI CCNNGG 4 cut(s) 850, 986, 1528, 1822
BssMI GATC 8 cut(s) 252, 432, 624, 726, 837, 1166, 1371, 1717
BssNI GRCGYC 1 cut(s) 1305
BssT1I CCWWGG 1 cut(s) 850
Bst2UI CCWGG 5 cut(s) 988, 1490, 1500, 1530, 1796
Bst4CI ACNGT 4 cut(s) 1000, 1396, 1432, 2256
Bst6I CTCTTC 1 cut(s) 1477
BstACI GRCGYC 1 cut(s) 1305
BstAFI CTTAAG 1 cut(s) 1655
BstAPI GCANNNNNTGC 3 cut(s) 662, 737, 1157
BstC8I GCNNGC 9 cut(s) 176, 208, 230, 443, 658, 1009, 1628, 1767, 1792
BstDEI CTNAG 7 cut(s) 38, 60, 279, 615, 1239, 1634, 1998
BstEII GGTNACC 1 cut(s) 1677
BstENI CCTNNNNNAGG 1 cut(s) 1188
BstF5I GGATG 6 cut(s) 33, 316, 454, 469, 829, 996
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 8 cut(s) 255, 435, 627, 729, 840, 1169, 1374, 1720
BstMAI GTCTC 2 cut(s) 765, 1184
BstMBI GATC 8 cut(s) 252, 432, 624, 726, 837, 1166, 1371, 1717
BstMCI CGRYCG 1 cut(s) 627
BstNI CCWGG 5 cut(s) 988, 1490, 1500, 1530, 1796
BstNSI RCATGY 1 cut(s) 1349
BstPI GGTNACC 1 cut(s) 1677
BstSCI CCNGG 7 cut(s) 986, 1488, 1498, 1528, 1794, 1821, 1822
BstSFI CTRYAG 1 cut(s) 1767
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 1 cut(s) 1166
BstXI CCANNNNNNTGG 1 cut(s) 376
BstYI RGATCY 1 cut(s) 1166
Bsu15I ATCGAT 1 cut(s) 627
Bsu36I CCTNAGG 1 cut(s) 615
BsuRI GGCC 4 cut(s) 954, 1482, 1527, 1794
BsuTUI ATCGAT 1 cut(s) 627
BtgZI GCGATG 1 cut(s) 1536
BtrI CACGTC 2 cut(s) 859, 1025
BtsCI GGATG 6 cut(s) 33, 316, 454, 469, 829, 996
BtsI GCAGTG 2 cut(s) 660, 885
BtsIMutI CAGTG 8 cut(s) 262, 660, 885, 1096, 1461, 1642, 1985, 2148
BveI ACCTGC 1 cut(s) 2168
Cac8I GCNNGC 9 cut(s) 176, 208, 230, 443, 658, 1009, 1628, 1767, 1792
CaiI CAGNNNCTG 1 cut(s) 788
CfoI GCGC 1 cut(s) 212
Cfr10I RCCGGY 1 cut(s) 1790
Cfr13I GGNCC 5 cut(s) 645, 953, 1481, 1526, 1991
Cfr9I CCCGGG 1 cut(s) 1822
ClaI ATCGAT 1 cut(s) 627
CseI GACGC 1 cut(s) 1294
Csp6I GTAC 3 cut(s) 241, 714, 1311
CviAII CATG 7 cut(s) 122, 641, 737, 1276, 1328, 1346, 2205
CviQI GTAC 3 cut(s) 241, 714, 1311
DdeI CTNAG 7 cut(s) 38, 60, 279, 615, 1239, 1634, 1998
DpnI GATC 8 cut(s) 254, 434, 626, 728, 839, 1168, 1373, 1719
DpnII GATC 8 cut(s) 252, 432, 624, 726, 837, 1166, 1371, 1717
EaeI YGGCCR 1 cut(s) 1792
Eam1104I CTCTTC 1 cut(s) 1477
EarI CTCTTC 1 cut(s) 1477
Ecl136II GAGCTC 2 cut(s) 73, 1662
Eco130I CCWWGG 1 cut(s) 850
Eco24I GRGCYC 3 cut(s) 75, 465, 1664
Eco31I GGTCTC 2 cut(s) 765, 1184
Eco47I GGWCC 2 cut(s) 645, 1991
Eco53kI GAGCTC 2 cut(s) 73, 1662
Eco57I CTGAAG 5 cut(s) 1182, 1341, 1388, 1745, 1971
Eco81I CCTNAGG 1 cut(s) 615
Eco88I CYCGRG 1 cut(s) 1822
Eco91I GGTNACC 1 cut(s) 1677
EcoICRI GAGCTC 2 cut(s) 73, 1662
EcoNI CCTNNNNNAGG 1 cut(s) 1188
EcoO109I RGGNCCY 2 cut(s) 645, 1481
EcoO65I GGTNACC 1 cut(s) 1677
EcoRI GAATTC 2 cut(s) 492, 2183
EcoRII CCWGG 5 cut(s) 986, 1488, 1498, 1528, 1794
EcoT14I CCWWGG 1 cut(s) 850
EcoT38I GRGCYC 3 cut(s) 75, 465, 1664
ErhI CCWWGG 1 cut(s) 850
FaeI CATG 7 cut(s) 125, 644, 740, 1279, 1331, 1349, 2208
FalI AAGNNNNNCTT 2 cut(s) 749, 781
FaqI GGGAC 2 cut(s) 658, 2004
FatI CATG 7 cut(s) 121, 640, 736, 1275, 1327, 1345, 2204
FauI CCCGC 2 cut(s) 514, 1635
FbaI TGATCA 2 cut(s) 252, 432
FokI GGATG 6 cut(s) 20, 323, 456, 461, 836, 1003
FriOI GRGCYC 3 cut(s) 75, 465, 1664
FspBI CTAG 2 cut(s) 270, 746
GlaI GCGC 1 cut(s) 211
GsaI CCCAGC 1 cut(s) 1488
GsuI CTGGAG 4 cut(s) 1149, 1284, 1437, 1551
HaeIII GGCC 4 cut(s) 954, 1482, 1527, 1794
HapII CCGG 5 cut(s) 396, 1337, 1791, 1823, 2237
HgaI GACGC 1 cut(s) 1294
HhaI GCGC 1 cut(s) 212
Hin1I GRCGYC 1 cut(s) 1305
Hin1II CATG 7 cut(s) 125, 644, 740, 1279, 1331, 1349, 2208
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HincII GTYRAC 1 cut(s) 1567
HindII GTYRAC 1 cut(s) 1567
HinfI GANTC 8 cut(s) 182, 410, 1073, 1145, 1279, 1442, 2196, 2233
HpaII CCGG 5 cut(s) 396, 1337, 1791, 1823, 2237
HphI GGTGA 8 cut(s) 69, 866, 1082, 1671, 2042, 2131, 2183, 2192
Hpy166II GTNNAC 2 cut(s) 1567, 2122
Hpy188I TCNGA 9 cut(s) 63, 351, 544, 770, 1439, 1509, 1714, 2001, 2019
Hpy188III TCNNGA 6 cut(s) 169, 281, 724, 1476, 1608, 1748
Hpy8I GTNNAC 2 cut(s) 1567, 2122
Hpy99I CGWCG 1 cut(s) 351
HpyCH4III ACNGT 4 cut(s) 1000, 1396, 1432, 2256
HpyCH4IV ACGT 3 cut(s) 114, 858, 1024
HpyF3I CTNAG 7 cut(s) 38, 60, 279, 615, 1239, 1634, 1998
HpySE526I ACGT 3 cut(s) 114, 858, 1024
Hsp92I GRCGYC 1 cut(s) 1305
Hsp92II CATG 7 cut(s) 125, 644, 740, 1279, 1331, 1349, 2208
HspAI GCGC 1 cut(s) 210
KflI GGGWCCC 1 cut(s) 645
KroI GCCGGC 1 cut(s) 1790
KroNI GCCGGC 1 cut(s) 1792
Ksp22I TGATCA 2 cut(s) 252, 432
Kzo9I GATC 8 cut(s) 252, 432, 624, 726, 837, 1166, 1371, 1717
LmnI GCTCC 1 cut(s) 1004
LweI GCATC 5 cut(s) 548, 775, 981, 1559, 1639
MaeI CTAG 2 cut(s) 270, 746
MaeII ACGT 3 cut(s) 114, 858, 1024
MaeIII GTNAC 8 cut(s) 52, 196, 527, 854, 1396, 1677, 1981, 2143
MalI GATC 8 cut(s) 254, 434, 626, 728, 839, 1168, 1373, 1719
MbiI CCGCTC 1 cut(s) 59
MboI GATC 8 cut(s) 252, 432, 624, 726, 837, 1166, 1371, 1717
MboII GAAGA 9 cut(s) 173, 257, 1259, 1304, 1325, 1381, 1464, 1712, 2140
MflI RGATCY 1 cut(s) 1166
MhlI GDGCHC 3 cut(s) 75, 465, 1664
MlyI GAGTC 1 cut(s) 2242
MmeI TCCRAC 2 cut(s) 329, 982
MroNI GCCGGC 1 cut(s) 1790
MroXI GAANNNNTTC 2 cut(s) 186, 1758
MslI CAYNNNNRTG 3 cut(s) 440, 1635, 2117
MspA1I CMGCKG 4 cut(s) 815, 941, 1361, 1685
MspCI CTTAAG 1 cut(s) 1655
MspI CCGG 5 cut(s) 396, 1337, 1791, 1823, 2237
MspR9I CCNGG 7 cut(s) 988, 1490, 1500, 1530, 1796, 1823, 1824
Mva1269I GAATGC 3 cut(s) 691, 709, 1539
MvaI CCWGG 5 cut(s) 988, 1490, 1500, 1530, 1796
NaeI GCCGGC 1 cut(s) 1792
NciI CCSGG 2 cut(s) 1823, 1824
NdeII GATC 8 cut(s) 252, 432, 624, 726, 837, 1166, 1371, 1717
NgoMIV GCCGGC 1 cut(s) 1790
NlaIII CATG 7 cut(s) 125, 644, 740, 1279, 1331, 1349, 2208
NlaIV GGNNCC 7 cut(s) 621, 646, 647, 1168, 1483, 1789, 1992
NmuCI GTSAC 5 cut(s) 527, 854, 1677, 1981, 2143
NspI RCATGY 1 cut(s) 1349
OliI CACNNNNGTG 1 cut(s) 2117
PaqCI CACCTGC 1 cut(s) 2168
PciI ACATGT 1 cut(s) 1345
PctI GAATGC 3 cut(s) 691, 709, 1539
PdiI GCCGGC 1 cut(s) 1792
PdmI GAANNNNTTC 2 cut(s) 186, 1758
PfeI GAWTC 7 cut(s) 182, 410, 1073, 1145, 1279, 1442, 2196
Pfl23II CGTACG 1 cut(s) 240
PflFI GACNNNGTC 1 cut(s) 1304
Ple19I CGATCG 1 cut(s) 627
PleI GAGTC 1 cut(s) 2241
PpsI GAGTC 1 cut(s) 2241
PpuMI RGGWCCY 1 cut(s) 645
PscI ACATGT 1 cut(s) 1345
PshBI ATTAAT 3 cut(s) 560, 1109, 1829
Psp124BI GAGCTC 2 cut(s) 75, 1664
Psp5II RGGWCCY 1 cut(s) 645
Psp6I CCWGG 5 cut(s) 986, 1488, 1498, 1528, 1794
PspEI GGTNACC 1 cut(s) 1677
PspFI CCCAGC 1 cut(s) 1484
PspGI CCWGG 5 cut(s) 986, 1488, 1498, 1528, 1794
PspLI CGTACG 1 cut(s) 240
PspN4I GGNNCC 7 cut(s) 621, 646, 647, 1168, 1483, 1789, 1992
PspPI GGNCC 5 cut(s) 645, 953, 1481, 1526, 1991
PspPPI RGGWCCY 1 cut(s) 645
PstI CTGCAG 1 cut(s) 1771
PstNI CAGNNNCTG 1 cut(s) 788
PsuI RGATCY 1 cut(s) 1166
PsyI GACNNNGTC 1 cut(s) 1304
PvuI CGATCG 1 cut(s) 627
PvuII CAGCTG 4 cut(s) 815, 941, 1361, 1685
RsaI GTAC 3 cut(s) 242, 715, 1312
RsaNI GTAC 3 cut(s) 241, 714, 1311
RseI CAYNNNNRTG 3 cut(s) 440, 1635, 2117
SacI GAGCTC 2 cut(s) 75, 1664
Sau3AI GATC 8 cut(s) 252, 432, 624, 726, 837, 1166, 1371, 1717
Sau96I GGNCC 5 cut(s) 645, 953, 1481, 1526, 1991
ScaI AGTACT 1 cut(s) 1312
SchI GAGTC 1 cut(s) 2242
ScrFI CCNGG 7 cut(s) 988, 1490, 1500, 1530, 1796, 1823, 1824
SduI GDGCHC 3 cut(s) 75, 465, 1664
SfaNI GCATC 5 cut(s) 548, 775, 981, 1559, 1639
SfcI CTRYAG 1 cut(s) 1767
SinI GGWCC 2 cut(s) 645, 1991
SmaI CCCGGG 1 cut(s) 1824
SmiMI CAYNNNNRTG 3 cut(s) 440, 1635, 2117
SmlI CTYRAG 2 cut(s) 167, 1655
SmoI CTYRAG 2 cut(s) 167, 1655
SsiI CCGC 4 cut(s) 57, 221, 507, 1628
SspI AATATT 1 cut(s) 924
SspMI CTAG 2 cut(s) 270, 746
SstI GAGCTC 2 cut(s) 75, 1664
StyD4I CCNGG 7 cut(s) 986, 1488, 1498, 1528, 1794, 1821, 1822
StyI CCWWGG 1 cut(s) 850
TaaI ACNGT 4 cut(s) 1000, 1396, 1432, 2256
TaiI ACGT 3 cut(s) 117, 861, 1027
TaqI TCGA 3 cut(s) 627, 1749, 1940
TatI WGTACW 1 cut(s) 1310
TfiI GAWTC 7 cut(s) 182, 410, 1073, 1145, 1279, 1442, 2196
TscAI CASTG 8 cut(s) 262, 667, 892, 1096, 1461, 1642, 1992, 2148
TseFI GTSAC 5 cut(s) 527, 854, 1677, 1981, 2143
Tsp45I GTSAC 5 cut(s) 527, 854, 1677, 1981, 2143
TspGWI ACGGA 2 cut(s) 335, 651
TspMI CCCGGG 1 cut(s) 1822
TspRI CASTG 8 cut(s) 262, 667, 892, 1096, 1461, 1642, 1992, 2148
Tth111I GACNNNGTC 1 cut(s) 1304
Vha464I CTTAAG 1 cut(s) 1655
VpaK11BI GGWCC 2 cut(s) 645, 1991
VspI ATTAAT 3 cut(s) 560, 1109, 1829
XagI CCTNNNNNAGG 1 cut(s) 1188
XapI RAATTY 6 cut(s) 415, 492, 946, 1387, 1726, 2183
XceI RCATGY 1 cut(s) 1349
XcmI CCANNNNNNNNNTGG 1 cut(s) 510
XmaI CCCGGG 1 cut(s) 1822
XmnI GAANNNNTTC 2 cut(s) 186, 1758
XspI CTAG 2 cut(s) 270, 746
ZrmI AGTACT 1 cut(s) 1312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.