RLG00000003746

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000302
Physical Location & Seq
Forward (+)
52242444 .. 52244259
1816 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003746

Sequence Viewer

Length: 1701 bp
ATGGTTTATGCATACCACAACGTGGCCACCGGATTTGCAGCAAAGCTGACCGCGGAGGAAGTTGAAGAGATGGAGAAGAAAGATGGGTTTATCTCGGCTCATCCAGAATGGAATATGCAATTGCACACAACTCATAGTCCTAACTTCTTGGGGCTGCAGCAAGGAATGGGACTTTGGAAAGGGTCAAATTATGGTGAAGGAGTGATTATTGGACTTTTGGATACTGGGATATTCCCAAACCATCCTTCATTTAGTGATGAAGGAGTACCACCTCCTCCAGCTAAATGGAAAGGCAAGTGTCATTTTAGCGGGACAGTCTGCAATAACAAGCTTATCGGCGCCAGAAGTTTTGATGGTGGAAAAACTACAGGAGGCCCTCCAGTTGACGATGAAGGTCATGGAACTCACACTTCAAGCGCAGCTGGAGGAAACTTTGTGAAATGTGCCAGTGTGTTTGGAAAGGCCAACGGCACAGCGACTGGCATGGCACCTTATGCTCACTTGGCAATGTACAAAGTCTGCTCTGAACAGGTCGGTGCATTTGGAGCAATTCAGAAGAGAATTTTTGTCAGCTGTTCGGCTGGAAATGAGGGACCTGACTACAAGACTTTATCAAATGAGGCCCCATGGATTCTCACAGTTGGAGCAAGCACCATTGACAGAAGCATAAGAGCAACAACAAAGCTTGGAAATAGGCAAGAATATGACGGGGAATCACTATTCCAGCCTAAAGATTTTACTTCAAAACTGTTACCTCTTATTTATACAGGTGCGCATAGCAATGATTCATCAGCTCTTTCCGATGTAGGGTCCCTTAAAAATGTTGAAGGCAAAGTAGTGGTGTGTGAGGTAGGTGGAGGAATTGGAAGAATTGCAAAAGGGGTAGAAGTGAAAAGAGTTGGTGGTGCTGCCATGATTCTTGTCAACCAAGACTTCGCTGGCTATACCACCATAGCAGACTCTCACGTGCTTTCGGCAACACATGTGAGTTACTCTGCAGGGGTGAGCATCAAAACCTATATAAACTTAACCTCAACTCCTACAGCAACAATCTTGTTCAAAGGCACTGTCATTGGTGATCAGCTTGCTCCGAAGGTCTATTTCTTTTCATCGAGAGGACCAAGCCTCGCAAGTCCTGGCATTTTGAAACCTGACATTATTGGTCCAGGTGTGAGCATCCTAGCTGCATGGCCTTTTTCAGTGGACAATGCCACAGAATCTAAAGCCACAGGATCTAAAGCCACATTAAACATTATTTCTGGTACTTCAATGTCATGCCCTCACCTAAGTGGCATTGCAGCCTTGCTCAAGAGCTCACACCCTGACTGGTCACCAGCTGCCATTAAGTCTGCCATCATGACAACCGCTGAAGTAAGCAACCTCGCAGGCTCCGCCATACTGGGTGAAACACTTTTTGCAGCAGACCTCTTTGCCATTGGTGCAGGTCATGTTAACCCTTCAAAAGCAAATGACCCTGGGCTCATCTACGACACGCAACCAGAGGACTACATTCCTTACTTGTGTGGTTTAAACTACACTGACAAACAGATAGCGGTAATCACCCAAAAATCAGTGAAATGCTCTGAAGAAGGAGCCATACCAGAAGCACAGCTAAATTATCCCACATTTTCTCTCTTTATAGATCCTGCTGGCAAGCCTCAGAAGTACACAAGAACAGTGACGAATTTTTGGCCCCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

567

Amino Acids

59.66

Weight (kDa)

6.57

Isoelectric Point (pI)

27.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 1 - 42 1.4e-06 Peptidase inhibitor I9
Peptidase_S8 PF00082 65 - 177 1.9e-09 Subtilase family
Peptidase_S8 PF00082 176 - 459 1.8e-22 Subtilase family
PA PF02225 266 - 336 2.6e-08 PA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 774
Acc36I ACCTGC 1 cut(s) 1433
AccB1I GGYRCC 2 cut(s) 338, 487
AccB7I CCANNNNNTGG 1 cut(s) 22
AccII CGCG 1 cut(s) 53
AciI CCGC 7 cut(s) 51, 53, 309, 1365, 1392, 1553, 1696
AclWI GGATC 2 cut(s) 1240, 1637
AcoI YGGCCR 1 cut(s) 24
AcsI RAATTY 2 cut(s) 561, 1684
AcuI CTGAAG 2 cut(s) 1389, 1605
AcvI CACGTG 1 cut(s) 967
AcyI GRCGYC 1 cut(s) 339
AdeI CACNNNGTG 1 cut(s) 22
AfaI GTAC 4 cut(s) 267, 512, 1264, 1667
AfiI CCNNNNNNNGG 2 cut(s) 22, 807
AflIII ACRYGT 1 cut(s) 982
AgsI TTSAA 8 cut(s) 65, 414, 744, 827, 1060, 1147, 1269, 1461
AjnI CCWGG 3 cut(s) 1135, 1165, 1474
AjuI GAANNNNNNNTTGG 4 cut(s) 157, 189, 229, 261
AleI CACNNNNGTG 1 cut(s) 1287
Alw21I GWGCWC 1 cut(s) 1316
AlwI GGATC 2 cut(s) 1240, 1637
AlwNI CAGNNNCTG 1 cut(s) 479
AoxI GGCC 6 cut(s) 24, 373, 462, 621, 1190, 1691
ApeKI GCWGC 9 cut(s) 38, 154, 157, 419, 908, 1184, 1298, 1337, 1418
ApoI RAATTY 2 cut(s) 561, 1684
ArsI GACNNNNNNTTYG 2 cut(s) 1053, 1085
AspLEI GCGC 3 cut(s) 341, 419, 775
AspS9I GGNCC 7 cut(s) 374, 593, 622, 810, 1118, 1163, 1692
AsuHPI GGTGA 7 cut(s) 206, 1015, 1088, 1274, 1323, 1415, 1552
AvaII GGWCC 4 cut(s) 593, 810, 1118, 1163
BalI TGGCCA 1 cut(s) 26
BanI GGYRCC 2 cut(s) 338, 487
BanII GRGCYC 2 cut(s) 1316, 1482
BarI GAAGNNNNNNTAC 2 cut(s) 1581, 1613
BbrPI CACGTG 1 cut(s) 967
Bbv12I GWGCWC 1 cut(s) 1316
BbvI GCAGC 9 cut(s) 50, 141, 169, 431, 895, 1171, 1310, 1324, 1430
BccI CCATC 5 cut(s) 64, 77, 249, 347, 1361
BceAI ACGGC 1 cut(s) 484
BciT130I CCWGG 3 cut(s) 1137, 1167, 1476
BciVI GTATCC 1 cut(s) 214
BclI TGATCA 1 cut(s) 1078
BfaI CTAG 1 cut(s) 1181
BfmI CTRYAG 4 cut(s) 155, 366, 996, 1041
BfoI RGCGCY 1 cut(s) 342
BfuAI ACCTGC 1 cut(s) 1433
BfuI GTATCC 1 cut(s) 214
BisI GCNGC 9 cut(s) 39, 155, 158, 420, 909, 1185, 1299, 1338, 1419
BlsI GCNGC 9 cut(s) 40, 156, 159, 421, 910, 1186, 1300, 1339, 1420
Bme1390I CCNGG 3 cut(s) 1137, 1167, 1476
Bme18I GGWCC 4 cut(s) 593, 810, 1118, 1163
BmgT120I GGNCC 7 cut(s) 374, 593, 622, 810, 1118, 1163, 1692
BmiI GGNNCC 9 cut(s) 340, 489, 594, 624, 811, 812, 1390, 1594, 1694
BmrFI CCNGG 3 cut(s) 1137, 1167, 1476
BmrI ACTGGG 2 cut(s) 234, 1409
BmsI GCATC 2 cut(s) 1017, 1185
BmuI ACTGGG 2 cut(s) 234, 1409
BpmI CTGGAG 3 cut(s) 261, 363, 444
BpuEI CTTGAG 1 cut(s) 1292
BsaAI YACGTR 1 cut(s) 967
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 4 cut(s) 51, 626, 1474, 1475
BsaWI WCCGGW 1 cut(s) 29
BsaXI ACNNNNNCTCC 2 cut(s) 1021, 1051
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 807
Bse1I ACTGG 6 cut(s) 229, 380, 447, 484, 1331, 1404
Bse3DI GCAATG 3 cut(s) 513, 787, 1293
BseBI CCWGG 3 cut(s) 1137, 1167, 1476
BseDI CCNNGG 4 cut(s) 51, 626, 1474, 1475
BseGI GGATG 3 cut(s) 100, 241, 1176
BseLI CCNNNNNNNGG 2 cut(s) 22, 807
BseMI GCAATG 3 cut(s) 513, 787, 1293
BseMII CTCAG 1 cut(s) 1673
BseNI ACTGG 6 cut(s) 229, 380, 447, 484, 1331, 1404
BseRI GAGGAG 1 cut(s) 264
BseXI GCAGC 9 cut(s) 50, 141, 169, 431, 895, 1171, 1310, 1324, 1430
BsgI GTGCAG 1 cut(s) 1461
Bsh1236I CGCG 1 cut(s) 53
BshFI GGCC 6 cut(s) 26, 375, 464, 623, 1192, 1693
BshNI GGYRCC 2 cut(s) 338, 487
BsiHKAI GWGCWC 1 cut(s) 1316
BsiSI CCGG 1 cut(s) 30
BslFI GGGAC 4 cut(s) 183, 325, 606, 796
BslI CCNNNNNNNGG 2 cut(s) 22, 807
BsmFI GGGAC 4 cut(s) 183, 325, 606, 796
BsnI GGCC 6 cut(s) 26, 375, 464, 623, 1192, 1693
Bsp1286I GDGCHC 2 cut(s) 1316, 1482
Bsp1407I TGTACA 1 cut(s) 510
Bsp143I GATC 3 cut(s) 1078, 1232, 1642
Bsp19I CCATGG 1 cut(s) 626
BspACI CCGC 7 cut(s) 51, 53, 309, 1365, 1392, 1553, 1696
BspANI GGCC 6 cut(s) 26, 375, 464, 623, 1192, 1693
BspCNI CTCAG 1 cut(s) 1672
BspFNI CGCG 1 cut(s) 53
BspHI TCATGA 1 cut(s) 1356
BspLI GGNNCC 9 cut(s) 340, 489, 594, 624, 811, 812, 1390, 1594, 1694
BspMAI CTGCAG 2 cut(s) 159, 1000
BspMI ACCTGC 1 cut(s) 1433
BspPI GGATC 2 cut(s) 1240, 1637
BspT107I GGYRCC 2 cut(s) 338, 487
BsrDI GCAATG 3 cut(s) 513, 787, 1293
BsrGI TGTACA 1 cut(s) 510
BsrI ACTGG 6 cut(s) 229, 380, 447, 484, 1331, 1404
BssECI CCNNGG 4 cut(s) 51, 626, 1474, 1475
BssMI GATC 3 cut(s) 1078, 1232, 1642
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 626
Bst2UI CCWGG 3 cut(s) 1137, 1167, 1476
Bst4CI ACNGT 5 cut(s) 316, 640, 750, 1069, 1678
Bst6I CTCTTC 2 cut(s) 60, 551
BstACI GRCGYC 1 cut(s) 339
BstAPI GCANNNNNTGC 1 cut(s) 494
BstAUI TGTACA 1 cut(s) 510
BstBAI YACGTR 1 cut(s) 967
BstC8I GCNNGC 6 cut(s) 649, 940, 1086, 1387, 1651, 1655
BstDEI CTNAG 2 cut(s) 1286, 1659
BstDSI CCRYGG 2 cut(s) 51, 626
BstEII GGTNACC 1 cut(s) 1329
BstF5I GGATG 3 cut(s) 100, 241, 1176
BstFNI CGCG 1 cut(s) 53
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 3 cut(s) 341, 419, 775
BstKTI GATC 3 cut(s) 1081, 1235, 1645
BstMBI GATC 3 cut(s) 1078, 1232, 1642
BstMWI GCNNNNNNNGC 5 cut(s) 494, 503, 545, 1391, 1439
BstNI CCWGG 3 cut(s) 1137, 1167, 1476
BstNSI RCATGY 1 cut(s) 986
BstPI GGTNACC 1 cut(s) 1329
BstSCI CCNGG 3 cut(s) 1135, 1165, 1474
BstSFI CTRYAG 4 cut(s) 155, 366, 996, 1041
BstUI CGCG 1 cut(s) 53
BstV1I GCAGC 9 cut(s) 50, 141, 169, 431, 895, 1171, 1310, 1324, 1430
BstX2I RGATCY 2 cut(s) 1232, 1642
BstXI CCANNNNNNTGG 1 cut(s) 285
BstYI RGATCY 2 cut(s) 1232, 1642
BsuI GTATCC 1 cut(s) 214
BsuRI GGCC 6 cut(s) 26, 375, 464, 623, 1192, 1693
BtgI CCRYGG 2 cut(s) 51, 626
BtsCI GGATG 3 cut(s) 100, 241, 1176
BtsIMutI CAGTG 6 cut(s) 454, 1065, 1206, 1536, 1578, 1683
BveI ACCTGC 1 cut(s) 1433
Cac8I GCNNGC 6 cut(s) 649, 940, 1086, 1387, 1651, 1655
CaiI CAGNNNCTG 1 cut(s) 479
CciI TCATGA 1 cut(s) 1356
CfoI GCGC 3 cut(s) 341, 419, 775
Cfr13I GGNCC 7 cut(s) 374, 593, 622, 810, 1118, 1163, 1692
Cfr42I CCGCGG 1 cut(s) 54
Csp6I GTAC 4 cut(s) 266, 511, 1263, 1666
CspCI CAANNNNNGTGG 2 cut(s) 16, 51
CviAII CATG 9 cut(s) 398, 484, 627, 913, 983, 1188, 1275, 1357, 1448
CviQI GTAC 4 cut(s) 266, 511, 1263, 1666
DdeI CTNAG 2 cut(s) 1286, 1659
DinI GGCGCC 1 cut(s) 340
DpnI GATC 3 cut(s) 1080, 1234, 1644
DpnII GATC 3 cut(s) 1078, 1232, 1642
DraI TTTAAA 1 cut(s) 1530
DraIII CACNNNGTG 1 cut(s) 22
EaeI YGGCCR 1 cut(s) 24
Eam1104I CTCTTC 2 cut(s) 60, 551
EarI CTCTTC 2 cut(s) 60, 551
EciI GGCGGA 1 cut(s) 1381
Ecl136II GAGCTC 1 cut(s) 1314
Eco130I CCWWGG 1 cut(s) 626
Eco24I GRGCYC 2 cut(s) 1316, 1482
Eco47I GGWCC 4 cut(s) 593, 810, 1118, 1163
Eco53kI GAGCTC 1 cut(s) 1314
Eco57I CTGAAG 2 cut(s) 1389, 1605
Eco72I CACGTG 1 cut(s) 967
Eco91I GGTNACC 1 cut(s) 1329
EcoICRI GAGCTC 1 cut(s) 1314
EcoO109I RGGNCCY 4 cut(s) 374, 593, 622, 810
EcoO65I GGTNACC 1 cut(s) 1329
EcoRII CCWGG 3 cut(s) 1135, 1165, 1474
EcoT14I CCWWGG 1 cut(s) 626
EcoT22I ATGCAT 1 cut(s) 13
EcoT38I GRGCYC 2 cut(s) 1316, 1482
EgeI GGCGCC 1 cut(s) 340
EheI GGCGCC 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 626
FaeI CATG 9 cut(s) 401, 487, 630, 916, 986, 1191, 1278, 1360, 1451
FaqI GGGAC 4 cut(s) 183, 325, 606, 796
FatI CATG 9 cut(s) 397, 483, 626, 912, 982, 1187, 1274, 1356, 1447
FauI CCCGC 1 cut(s) 302
FbaI TGATCA 1 cut(s) 1078
Fnu4HI GCNGC 9 cut(s) 39, 155, 158, 420, 909, 1185, 1299, 1338, 1419
FokI GGATG 3 cut(s) 87, 228, 1163
FriOI GRGCYC 2 cut(s) 1316, 1482
Fsp4HI GCNGC 9 cut(s) 39, 155, 158, 420, 909, 1185, 1299, 1338, 1419
FspAI RTGCGCAY 1 cut(s) 774
FspBI CTAG 1 cut(s) 1181
FspI TGCGCA 1 cut(s) 774
GlaI GCGC 3 cut(s) 340, 418, 774
GluI GCNGC 9 cut(s) 39, 155, 158, 420, 909, 1185, 1299, 1338, 1419
GsuI CTGGAG 3 cut(s) 261, 363, 444
HaeII RGCGCY 1 cut(s) 342
HaeIII GGCC 6 cut(s) 26, 375, 464, 623, 1192, 1693
HapII CCGG 1 cut(s) 30
HhaI GCGC 3 cut(s) 341, 419, 775
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 9 cut(s) 401, 487, 630, 916, 986, 1191, 1278, 1360, 1451
Hin6I GCGC 3 cut(s) 339, 417, 773
HinP1I GCGC 3 cut(s) 339, 417, 773
HincII GTYRAC 3 cut(s) 385, 925, 1453
HindII GTYRAC 3 cut(s) 385, 925, 1453
HindIII AAGCTT 2 cut(s) 329, 683
HinfI GANTC 6 cut(s) 631, 713, 785, 916, 959, 1217
HpaI GTTAAC 1 cut(s) 1453
HpaII CCGG 1 cut(s) 30
HphI GGTGA 7 cut(s) 206, 1015, 1088, 1274, 1323, 1415, 1552
Hpy166II GTNNAC 5 cut(s) 385, 925, 1204, 1453, 1668
Hpy188I TCNGA 6 cut(s) 526, 555, 802, 1092, 1585, 1662
Hpy188III TCNNGA 4 cut(s) 104, 1113, 1309, 1357
Hpy8I GTNNAC 5 cut(s) 385, 925, 1204, 1453, 1668
HpyAV CCTTC 8 cut(s) 191, 254, 255, 386, 821, 1087, 1467, 1583
HpyCH4III ACNGT 5 cut(s) 316, 640, 750, 1069, 1678
HpyCH4IV ACGT 2 cut(s) 21, 966
HpyF10VI GCNNNNNNNGC 5 cut(s) 494, 503, 545, 1391, 1439
HpyF3I CTNAG 2 cut(s) 1286, 1659
HpySE526I ACGT 2 cut(s) 21, 966
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 9 cut(s) 401, 487, 630, 916, 986, 1191, 1278, 1360, 1451
HspAI GCGC 3 cut(s) 339, 417, 773
KasI GGCGCC 1 cut(s) 338
KflI GGGWCCC 1 cut(s) 810
Ksp22I TGATCA 1 cut(s) 1078
KspAI GTTAAC 1 cut(s) 1453
KspI CCGCGG 1 cut(s) 54
Kzo9I GATC 3 cut(s) 1078, 1232, 1642
LmnI GCTCC 5 cut(s) 545, 644, 1093, 1394, 1592
Lsp1109I GCAGC 9 cut(s) 50, 141, 169, 431, 895, 1171, 1310, 1324, 1430
LweI GCATC 2 cut(s) 1017, 1185
MaeI CTAG 1 cut(s) 1181
MaeII ACGT 2 cut(s) 21, 966
MaeIII GTNAC 4 cut(s) 750, 989, 1329, 1678
MalI GATC 3 cut(s) 1080, 1234, 1644
MboI GATC 3 cut(s) 1078, 1232, 1642
MboII GAAGA 5 cut(s) 77, 88, 568, 879, 1598
MfeI CAATTG 1 cut(s) 119
MflI RGATCY 2 cut(s) 1232, 1642
MhlI GDGCHC 2 cut(s) 1316, 1482
MlsI TGGCCA 1 cut(s) 26
MluCI AATT 8 cut(s) 119, 187, 549, 561, 861, 870, 1615, 1684
MluNI TGGCCA 1 cut(s) 26
Mly113I GGCGCC 1 cut(s) 339
MlyI GAGTC 1 cut(s) 953
MmeI TCCRAC 1 cut(s) 622
Mox20I TGGCCA 1 cut(s) 26
Mph1103I ATGCAT 1 cut(s) 13
MscI TGGCCA 1 cut(s) 26
MseI TTAA 6 cut(s) 816, 1028, 1247, 1344, 1452, 1529
MslI CAYNNNNRTG 2 cut(s) 780, 1287
Msp20I TGGCCA 1 cut(s) 26
MspA1I CMGCKG 5 cut(s) 53, 422, 573, 1337, 1367
MspI CCGG 1 cut(s) 30
MspR9I CCNGG 3 cut(s) 1137, 1167, 1476
MssI GTTTAAAC 1 cut(s) 1530
MunI CAATTG 1 cut(s) 119
MvaI CCWGG 3 cut(s) 1137, 1167, 1476
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 5 cut(s) 494, 503, 545, 1391, 1439
NarI GGCGCC 1 cut(s) 339
NcoI CCATGG 1 cut(s) 626
NdeII GATC 3 cut(s) 1078, 1232, 1642
NlaIII CATG 9 cut(s) 401, 487, 630, 916, 986, 1191, 1278, 1360, 1451
NlaIV GGNNCC 9 cut(s) 340, 489, 594, 624, 811, 812, 1390, 1594, 1694
NmeAIII GCCGAG 1 cut(s) 74
NmuCI GTSAC 2 cut(s) 1329, 1678
NsbI TGCGCA 1 cut(s) 774
NsiI ATGCAT 1 cut(s) 13
NspI RCATGY 1 cut(s) 986
OliI CACNNNNGTG 1 cut(s) 1287
PagI TCATGA 1 cut(s) 1356
PasI CCCWGGG 1 cut(s) 1475
PciI ACATGT 1 cut(s) 982
PfeI GAWTC 5 cut(s) 631, 713, 785, 916, 1217
PflMI CCANNNNNTGG 1 cut(s) 22
PkrI GCNGC 9 cut(s) 40, 156, 159, 421, 910, 1186, 1300, 1339, 1420
PleI GAGTC 1 cut(s) 953
PluTI GGCGCC 1 cut(s) 342
PmaCI CACGTG 1 cut(s) 967
PmeI GTTTAAAC 1 cut(s) 1530
PmlI CACGTG 1 cut(s) 967
PpsI GAGTC 1 cut(s) 953
Ppu21I YACGTR 1 cut(s) 967
PpuMI RGGWCCY 2 cut(s) 593, 810
PscI ACATGT 1 cut(s) 982
Psp124BI GAGCTC 1 cut(s) 1316
Psp5II RGGWCCY 2 cut(s) 593, 810
Psp6I CCWGG 3 cut(s) 1135, 1165, 1474
PspCI CACGTG 1 cut(s) 967
PspEI GGTNACC 1 cut(s) 1329
PspGI CCWGG 3 cut(s) 1135, 1165, 1474
PspN4I GGNNCC 9 cut(s) 340, 489, 594, 624, 811, 812, 1390, 1594, 1694
PspPI GGNCC 7 cut(s) 374, 593, 622, 810, 1118, 1163, 1692
PspPPI RGGWCCY 2 cut(s) 593, 810
PstI CTGCAG 2 cut(s) 159, 1000
PstNI CAGNNNCTG 1 cut(s) 479
PsuI RGATCY 2 cut(s) 1232, 1642
PvuII CAGCTG 3 cut(s) 422, 573, 1337
RsaI GTAC 4 cut(s) 267, 512, 1264, 1667
RsaNI GTAC 4 cut(s) 266, 511, 1263, 1666
RseI CAYNNNNRTG 2 cut(s) 780, 1287
SacI GAGCTC 1 cut(s) 1316
SacII CCGCGG 1 cut(s) 54
SaqAI TTAA 6 cut(s) 816, 1028, 1247, 1344, 1452, 1529
SatI GCNGC 9 cut(s) 39, 155, 158, 420, 909, 1185, 1299, 1338, 1419
Sau3AI GATC 3 cut(s) 1078, 1232, 1642
Sau96I GGNCC 7 cut(s) 374, 593, 622, 810, 1118, 1163, 1692
SchI GAGTC 1 cut(s) 953
ScrFI CCNGG 3 cut(s) 1137, 1167, 1476
SduI GDGCHC 2 cut(s) 1316, 1482
SfaNI GCATC 2 cut(s) 1017, 1185
SfcI CTRYAG 4 cut(s) 155, 366, 996, 1041
SfoI GGCGCC 1 cut(s) 340
Sfr303I CCGCGG 1 cut(s) 54
SgrBI CCGCGG 1 cut(s) 54
SinI GGWCC 4 cut(s) 593, 810, 1118, 1163
SmiMI CAYNNNNRTG 2 cut(s) 780, 1287
SmlI CTYRAG 1 cut(s) 1307
SmoI CTYRAG 1 cut(s) 1307
Sse9I AATT 8 cut(s) 119, 187, 549, 561, 861, 870, 1615, 1684
SsiI CCGC 7 cut(s) 51, 53, 309, 1365, 1392, 1553, 1696
SspDI GGCGCC 1 cut(s) 338
SspMI CTAG 1 cut(s) 1181
SstI GAGCTC 1 cut(s) 1316
StyD4I CCNGG 3 cut(s) 1135, 1165, 1474
StyI CCWWGG 1 cut(s) 626
TaaI ACNGT 5 cut(s) 316, 640, 750, 1069, 1678
TaiI ACGT 2 cut(s) 24, 969
TaqI TCGA 1 cut(s) 1112
TasI AATT 8 cut(s) 119, 187, 549, 561, 861, 870, 1615, 1684
TatI WGTACW 2 cut(s) 510, 1665
TfiI GAWTC 5 cut(s) 631, 713, 785, 916, 1217
Tru1I TTAA 6 cut(s) 816, 1028, 1247, 1344, 1452, 1529
Tru9I TTAA 6 cut(s) 816, 1028, 1247, 1344, 1452, 1529
TscAI CASTG 6 cut(s) 454, 1072, 1206, 1543, 1578, 1683
TseFI GTSAC 2 cut(s) 1329, 1678
TseI GCWGC 9 cut(s) 38, 154, 157, 419, 908, 1184, 1298, 1337, 1418
Tsp45I GTSAC 2 cut(s) 1329, 1678
TspDTI ATGAA 5 cut(s) 237, 273, 405, 777, 1098
TspRI CASTG 6 cut(s) 454, 1072, 1206, 1543, 1578, 1683
Van91I CCANNNNNTGG 1 cut(s) 22
VpaK11BI GGWCC 4 cut(s) 593, 810, 1118, 1163
XapI RAATTY 2 cut(s) 561, 1684
XceI RCATGY 1 cut(s) 986
XcmI CCANNNNNNNNNTGG 1 cut(s) 935
XspI CTAG 1 cut(s) 1181
Zsp2I ATGCAT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.