RLG00000003747

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000302
Physical Location & Seq
Forward (+)
52246746 .. 52252814
6069 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003747

Sequence Viewer

Length: 2298 bp
ATGAAGCCACGAATGGTTTACGCATACCACAATGTGGCCACAGGATTTGCAGCAAAGCTGACTGGGGAGGAAGTTGAAGAAATGGAGAAGAAAGATGGGTTTATCTACGCTCATCCAGAACGGAATCTGCAATTGCACACAACTCATAGTCCTAACTTCTTGGGGCTGCAACAAGGATTGGGACTTTGGAAAGGGTCTAATTATGGTGCAGGAATCATTATTGGAGTTTTGGATACTGGAATAACACCAGGTCATCCCTCATTCAGCGACGAAGGAGTGCCACCTCCTCCTGCTAAATGGAAAGGCAAGTGTGATTTCAATGGCACATTCTGCAACAACAAGCTTATTGGTGCCAGAAGTTTCCAGGGTGGGAACACTACCGGAGCTCCACCAGTTGACATTGCAGGCCATGGAACCCACACGTCGAGCACAGCTGGAGGAAATTTTGTGAAAGGTGCCAATGTGTTTGGAATGGCAAATGGAACAGCCTCTGGCATGGCACCTTATGCTCACTTGGCAATGTACCAAGTTTGTTCTGAACAAGGTTGTTCTGAAAGTGACATTCTAGCTGCTATTGACACTGCTATTGATGATGGCGTGGATGTGCTATCCCTCTCACTTGGTGATGGCTCAGCTACTTTTTATTGGGATTTTATTGCAATTGGTGCATTTGCAGCAATTCAGAAGGGAATTTTTGTCAGCTGTTCGGCTGGAAATGCGGGGCCTGACTACTCTACTACATCAAATGAGGCCCCATGGATTTTGACAGTTGGAGCAAGCTCCATTGACAGAAACATAAGAGCAATAGCACAGCTTGGAAATGGGCAAGAATATGATGGGGAATCACTATTCCAGCCTAAAGATTTTAGTTCAAAACAGTTACCTCTTGTTTATGCAGGTGACCATAGCCATGATTCATCAGCTCTTTGCGATGTAGGGTCCCTTACAAATGTTGAAGGGAAAGTAGTGGTGTGTGAGCTAGGTGGCGGAATTGGAAGAATTGCAAAAGGGGTAGAAGTGAAAAGAGCTGGTGGTGTTGCCATGATTCTTGTCAACCCAGACTTTGCTGGCTATACCACCATAGCAGACGCTCACGTGCTTCCGGCAACACATGTGAGTTACTCTGCAGGGGTGAGTATCAAAACCTATATAAACTCAACCTCAATTCCTACAGCAACAATCTTGTTCAAAGGCACTGTCATTGGTGATCAGCTTGCTCCGAAGGTCTCTTTCTTTTCATCAAGAGGACCAAGCCTCGCAAGTCCTGGCATTTTGAAACCTGACATAATTGGTCCTGGTGTGAGCATCCTAGCTGCATGGCCTTTTTCGGTGGACAATGCCACAGAATCTAAAGCCACATTTAACATTATTTCCGGTACTTCAATGTCATGCCCTCACCTGAGTGGCATTGTAGCCTTACTCAAGAGCTCACACCCTGACTGGTCACCAGCTGCCATTAAGTCTGCCATCATGACAACTGCTGAAGTAAACAACCTCGCAGGCTCCGCCATAATGGATGAAACACTATTTACAGCAGACCTCTTTGCCATTGGTGCAGGTCATGTTAACCCTTCAAAAGCAAATGACCCTGGGCTCATCTACGACACGCAACCAGATGACTACATTCCTTACATGTGTGGTTTGAACTACACAGACCAACAGATAGCAATAGTCACCCGGAAATTAGTGAAATGCTCTGAAGTAGGAGCCATACCAGAAGCACAGCTAAATTATCCCACATTTTCTCTCTTTATAGATCCTGCTGGGAAGCCTCAAAATTACACAAGAACAGTGACGAATGTTGGCCCCGCTAAATCAACTTATGAATTAGCCGTTGTAAGTCCACATAACATAGACATCAGTGTGCAACCTGAGGAGCTCACATTCACAGAGGTTAACCAGACAATGACGTATCATGTGGTGTTTACCGCACAAAAAGGTGCTGCGAAGGATGGTATAAAATTTTCTCAGGGATATCTGAGCTGGGTCTCTCGCCAGCATTCTGTTAGAAGCCAAATATCGCACTGGCTCAAGAGCTCACACCCTACCTGGACGCCGGCTGCCATTAAGTCTGCAATCAAGACAACCGCTGATATACTAAACCTCGGAGGCAAGGCCATTGTTGATGAAAGACATGCTCCAGCAGACATCTTTGCCACTGGTGCAGGCCATGTCAAACCTTCAAAAGCAAATGACCCTGGGCTCATCTATGACCTAAACCCTGAAGATTACATCCCCTACTTGTGCGGTTTGAATTATACAGATGACCAGATACTGATATTACCCATGAAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

766

Amino Acids

80.91

Weight (kDa)

5.62

Isoelectric Point (pI)

29.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 3 - 46 9.3e-07 Peptidase inhibitor I9
Peptidase_S8 PF00082 69 - 497 3.7e-46 Subtilase family
PA PF02225 293 - 379 6e-11 PA domain
fn3_6 PF17766 575 - 673 7.4e-26 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 887
Acc36I ACCTGC 2 cut(s) 887, 1547
AccB1I GGYRCC 3 cut(s) 350, 455, 499
AccB7I CCANNNNNTGG 1 cut(s) 34
AciI CCGC 7 cut(s) 719, 987, 1506, 1809, 1929, 2088, 2247
AclWI GGATC 1 cut(s) 1751
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 3 cut(s) 442, 690, 1961
AcuI CTGAAG 3 cut(s) 1503, 1719, 2244
AcvI CACGTG 1 cut(s) 1096
AcyI GRCGYC 1 cut(s) 2054
AdeI CACNNNGTG 2 cut(s) 34, 623
AfaI GTAC 2 cut(s) 524, 1378
AfiI CCNNNNNNNGG 1 cut(s) 34
AflIII ACRYGT 3 cut(s) 420, 1111, 1632
AjiI CACGTC 1 cut(s) 423
AjnI CCWGG 7 cut(s) 247, 363, 1264, 1294, 1588, 2048, 2197
AleI CACNNNNGTG 1 cut(s) 1401
Alw21I GWGCWC 5 cut(s) 388, 431, 1430, 1881, 2039
Alw26I GTCTC 2 cut(s) 1231, 1993
AlwI GGATC 1 cut(s) 1751
AlwNI CAGNNNCTG 2 cut(s) 491, 2275
AoxI GGCC 8 cut(s) 36, 406, 722, 750, 1319, 1804, 2115, 2167
ApeKI GCWGC 8 cut(s) 50, 166, 569, 674, 1313, 1451, 1943, 2060
ApoI RAATTY 3 cut(s) 442, 690, 1961
ArsI GACNNNNNNTTYG 2 cut(s) 1182, 1214
AspS9I GGNCC 6 cut(s) 722, 751, 939, 1247, 1292, 1805
AsuC2I CCSGG 1 cut(s) 1678
AsuHPI GGTGA 7 cut(s) 635, 911, 1144, 1217, 1388, 1437, 1666
AvaII GGWCC 3 cut(s) 939, 1247, 1292
AxyI CCTNAGG 1 cut(s) 1872
BalI TGGCCA 1 cut(s) 38
BanI GGYRCC 3 cut(s) 350, 455, 499
BanII GRGCYC 6 cut(s) 388, 1430, 1596, 1881, 2039, 2205
BbrPI CACGTG 1 cut(s) 1096
Bbv12I GWGCWC 5 cut(s) 388, 431, 1430, 1881, 2039
BbvI GCAGC 8 cut(s) 62, 153, 556, 686, 1300, 1438, 1930, 2047
BccI CCATC 6 cut(s) 89, 587, 620, 830, 1475, 1946
BceAI ACGGC 1 cut(s) 1817
BciT130I CCWGG 7 cut(s) 249, 365, 1266, 1296, 1590, 2050, 2199
BciVI GTATCC 1 cut(s) 226
BclI TGATCA 1 cut(s) 1207
BcnI CCSGG 1 cut(s) 1678
BcoDI GTCTC 2 cut(s) 1231, 1993
BfaI CTAG 3 cut(s) 566, 980, 1310
BfmI CTRYAG 2 cut(s) 1125, 1170
BfuAI ACCTGC 2 cut(s) 887, 1547
BfuI GTATCC 1 cut(s) 226
BisI GCNGC 8 cut(s) 51, 167, 570, 675, 1314, 1452, 1944, 2061
BlpI GCTNAGC 1 cut(s) 631
BlsI GCNGC 8 cut(s) 52, 168, 571, 676, 1315, 1453, 1945, 2062
Bme1390I CCNGG 8 cut(s) 249, 365, 1266, 1296, 1590, 1678, 2050, 2199
Bme18I GGWCC 3 cut(s) 939, 1247, 1292
BmgBI CACGTC 1 cut(s) 423
BmgT120I GGNCC 6 cut(s) 722, 751, 939, 1247, 1292, 1805
BmrFI CCNGG 8 cut(s) 249, 365, 1266, 1296, 1590, 1678, 2050, 2199
BmrI ACTGGG 1 cut(s) 72
BmsI GCATC 1 cut(s) 1314
BmuI ACTGGG 1 cut(s) 72
BpmI CTGGAG 2 cut(s) 456, 2124
Bpu1102I GCTNAGC 1 cut(s) 631
BpuEI CTTGAG 2 cut(s) 1406, 2015
BpuMI CCSGG 1 cut(s) 1678
BsaAI YACGTR 1 cut(s) 1096
BsaHI GRCGYC 1 cut(s) 2054
BsaI GGTCTC 2 cut(s) 1231, 1993
BsaJI CCNNGG 8 cut(s) 364, 409, 755, 1588, 1589, 2104, 2197, 2198
BsaWI WCCGGW 2 cut(s) 380, 1373
BsaXI ACNNNNNCTCC 2 cut(s) 370, 400
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse118I RCCGGY 1 cut(s) 2056
Bse1I ACTGG 6 cut(s) 67, 241, 392, 1445, 2030, 2164
Bse21I CCTNAGG 1 cut(s) 1872
Bse3DI GCAATG 2 cut(s) 399, 525
BseBI CCWGG 7 cut(s) 249, 365, 1266, 1296, 1590, 2050, 2199
BseDI CCNNGG 8 cut(s) 364, 409, 755, 1588, 1589, 2104, 2197, 2198
BseGI GGATG 7 cut(s) 112, 253, 607, 1305, 1522, 1957, 2232
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMI GCAATG 2 cut(s) 399, 525
BseMII CTCAG 5 cut(s) 645, 1391, 1863, 1970, 1982
BseNI ACTGG 6 cut(s) 67, 241, 392, 1445, 2030, 2164
BseRI GAGGAG 2 cut(s) 276, 1889
BseXI GCAGC 8 cut(s) 62, 153, 556, 686, 1300, 1438, 1930, 2047
BseYI CCCAGC 2 cut(s) 1763, 1983
BsgI GTGCAG 3 cut(s) 228, 1575, 2184
BshFI GGCC 8 cut(s) 38, 408, 724, 752, 1321, 1806, 2117, 2169
BshNI GGYRCC 3 cut(s) 350, 455, 499
BsiHKAI GWGCWC 5 cut(s) 388, 431, 1430, 1881, 2039
BsiSI CCGG 5 cut(s) 381, 1103, 1374, 1678, 2057
BslFI GGGAC 2 cut(s) 195, 925
BslI CCNNNNNNNGG 1 cut(s) 34
BsmAI GTCTC 2 cut(s) 1231, 1993
BsmFI GGGAC 2 cut(s) 195, 925
BsmI GAATGC 1 cut(s) 1999
BsnI GGCC 8 cut(s) 38, 408, 724, 752, 1321, 1806, 2117, 2169
Bso31I GGTCTC 2 cut(s) 1231, 1993
Bsp1286I GDGCHC 7 cut(s) 388, 431, 1430, 1596, 1881, 2039, 2205
Bsp143I GATC 2 cut(s) 1207, 1756
Bsp1720I GCTNAGC 1 cut(s) 631
Bsp19I CCATGG 2 cut(s) 409, 755
BspACI CCGC 7 cut(s) 719, 987, 1506, 1809, 1929, 2088, 2247
BspANI GGCC 8 cut(s) 38, 408, 724, 752, 1321, 1806, 2117, 2169
BspCNI CTCAG 5 cut(s) 644, 1392, 1864, 1971, 1981
BspHI TCATGA 1 cut(s) 1470
BspMAI CTGCAG 1 cut(s) 1129
BspMI ACCTGC 2 cut(s) 887, 1547
BspPI GGATC 1 cut(s) 1751
BspT107I GGYRCC 3 cut(s) 350, 455, 499
BspTNI GGTCTC 2 cut(s) 1231, 1993
BsrDI GCAATG 2 cut(s) 399, 525
BsrFI RCCGGY 1 cut(s) 2056
BsrI ACTGG 6 cut(s) 67, 241, 392, 1445, 2030, 2164
BssAI RCCGGY 1 cut(s) 2056
BssECI CCNNGG 8 cut(s) 364, 409, 755, 1588, 1589, 2104, 2197, 2198
BssMI GATC 2 cut(s) 1207, 1756
BssNI GRCGYC 1 cut(s) 2054
BssT1I CCWWGG 2 cut(s) 409, 755
Bst2UI CCWGG 7 cut(s) 249, 365, 1266, 1296, 1590, 2050, 2199
Bst4CI ACNGT 5 cut(s) 769, 879, 1198, 1792, 2295
BstACI GRCGYC 1 cut(s) 2054
BstAPI GCANNNNNTGC 3 cut(s) 330, 506, 665
BstBAI YACGTR 1 cut(s) 1096
BstC8I GCNNGC 8 cut(s) 406, 778, 1069, 1215, 1501, 1997, 2058, 2167
BstDEI CTNAG 5 cut(s) 631, 1400, 1872, 1968, 1979
BstDSI CCRYGG 2 cut(s) 409, 755
BstEII GGTNACC 2 cut(s) 899, 1443
BstF5I GGATG 7 cut(s) 112, 253, 607, 1305, 1522, 1957, 2232
BstKTI GATC 2 cut(s) 1210, 1759
BstMAI GTCTC 2 cut(s) 1231, 1993
BstMBI GATC 2 cut(s) 1207, 1756
BstMWI GCNNNNNNNGC 9 cut(s) 330, 506, 515, 665, 674, 716, 1505, 1553, 2162
BstNI CCWGG 7 cut(s) 249, 365, 1266, 1296, 1590, 2050, 2199
BstNSI RCATGY 3 cut(s) 1115, 1636, 2138
BstPI GGTNACC 2 cut(s) 899, 1443
BstSCI CCNGG 8 cut(s) 247, 363, 1264, 1294, 1588, 1676, 2048, 2197
BstSFI CTRYAG 2 cut(s) 1125, 1170
BstV1I GCAGC 8 cut(s) 62, 153, 556, 686, 1300, 1438, 1930, 2047
BstX2I RGATCY 1 cut(s) 1756
BstYI RGATCY 1 cut(s) 1756
Bsu36I CCTNAGG 1 cut(s) 1872
BsuI GTATCC 1 cut(s) 226
BsuRI GGCC 8 cut(s) 38, 408, 724, 752, 1321, 1806, 2117, 2169
BtgI CCRYGG 2 cut(s) 409, 755
BtgZI GCGATG 1 cut(s) 945
BtrI CACGTC 1 cut(s) 423
BtsCI GGATG 7 cut(s) 112, 253, 607, 1305, 1522, 1957, 2232
BtsI GCAGTG 1 cut(s) 579
BtsIMutI CAGTG 6 cut(s) 579, 1194, 1797, 1867, 2023, 2157
BveI ACCTGC 2 cut(s) 887, 1547
Cac8I GCNNGC 8 cut(s) 406, 778, 1069, 1215, 1501, 1997, 2058, 2167
CaiI CAGNNNCTG 2 cut(s) 491, 2275
CciI TCATGA 1 cut(s) 1470
Cfr10I RCCGGY 1 cut(s) 2056
Cfr13I GGNCC 6 cut(s) 722, 751, 939, 1247, 1292, 1805
CseI GACGC 2 cut(s) 1097, 2062
CsiI ACCWGGT 1 cut(s) 247
Csp6I GTAC 2 cut(s) 523, 1377
CspCI CAANNNNNGTGG 2 cut(s) 28, 63
CviQI GTAC 2 cut(s) 523, 1377
DdeI CTNAG 5 cut(s) 631, 1400, 1872, 1968, 1979
DpnI GATC 2 cut(s) 1209, 1758
DpnII GATC 2 cut(s) 1207, 1756
DraIII CACNNNGTG 2 cut(s) 34, 623
EaeI YGGCCR 1 cut(s) 36
EciI GGCGGA 2 cut(s) 1002, 1495
Ecl136II GAGCTC 4 cut(s) 386, 1428, 1879, 2037
Eco130I CCWWGG 2 cut(s) 409, 755
Eco24I GRGCYC 6 cut(s) 388, 1430, 1596, 1881, 2039, 2205
Eco31I GGTCTC 2 cut(s) 1231, 1993
Eco32I GATATC 1 cut(s) 1976
Eco47I GGWCC 3 cut(s) 939, 1247, 1292
Eco53kI GAGCTC 4 cut(s) 386, 1428, 1879, 2037
Eco57I CTGAAG 3 cut(s) 1503, 1719, 2244
Eco72I CACGTG 1 cut(s) 1096
Eco81I CCTNAGG 1 cut(s) 1872
Eco91I GGTNACC 2 cut(s) 899, 1443
EcoICRI GAGCTC 4 cut(s) 386, 1428, 1879, 2037
EcoO109I RGGNCCY 3 cut(s) 722, 751, 939
EcoO65I GGTNACC 2 cut(s) 899, 1443
EcoRII CCWGG 7 cut(s) 247, 363, 1264, 1294, 1588, 2048, 2197
EcoRV GATATC 1 cut(s) 1976
EcoT14I CCWWGG 2 cut(s) 409, 755
EcoT38I GRGCYC 6 cut(s) 388, 1430, 1596, 1881, 2039, 2205
ErhI CCWWGG 2 cut(s) 409, 755
FaqI GGGAC 2 cut(s) 195, 925
FauI CCCGC 2 cut(s) 712, 1816
FbaI TGATCA 1 cut(s) 1207
Fnu4HI GCNGC 8 cut(s) 51, 167, 570, 675, 1314, 1452, 1944, 2061
FokI GGATG 7 cut(s) 99, 240, 614, 1292, 1529, 1964, 2219
FriOI GRGCYC 6 cut(s) 388, 1430, 1596, 1881, 2039, 2205
Fsp4HI GCNGC 8 cut(s) 51, 167, 570, 675, 1314, 1452, 1944, 2061
FspBI CTAG 3 cut(s) 566, 980, 1310
GluI GCNGC 8 cut(s) 51, 167, 570, 675, 1314, 1452, 1944, 2061
GsaI CCCAGC 2 cut(s) 1767, 1987
GsuI CTGGAG 2 cut(s) 456, 2124
HaeIII GGCC 8 cut(s) 38, 408, 724, 752, 1321, 1806, 2117, 2169
HapII CCGG 5 cut(s) 381, 1103, 1374, 1678, 2057
HgaI GACGC 2 cut(s) 1097, 2062
Hin1I GRCGYC 1 cut(s) 2054
HincII GTYRAC 4 cut(s) 397, 1054, 1567, 1897
HindII GTYRAC 4 cut(s) 397, 1054, 1567, 1897
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 6 cut(s) 124, 213, 842, 914, 1045, 1346
HpaI GTTAAC 2 cut(s) 1567, 1897
HpaII CCGG 5 cut(s) 381, 1103, 1374, 1678, 2057
HphI GGTGA 7 cut(s) 635, 911, 1144, 1217, 1388, 1437, 1666
Hpy166II GTNNAC 9 cut(s) 19, 397, 1054, 1333, 1489, 1567, 1844, 1897, 1926
Hpy188I TCNGA 7 cut(s) 538, 553, 684, 1221, 1699, 1980, 2108
Hpy188III TCNNGA 6 cut(s) 116, 1242, 1423, 1471, 2032, 2080
Hpy8I GTNNAC 9 cut(s) 19, 397, 1054, 1333, 1489, 1567, 1844, 1897, 1926
Hpy99I CGWCG 2 cut(s) 272, 427
HpyAV CCTTC 7 cut(s) 266, 679, 950, 1216, 1581, 1942, 2190
HpyCH4III ACNGT 5 cut(s) 769, 879, 1198, 1792, 2295
HpyCH4IV ACGT 3 cut(s) 422, 1095, 1910
HpyF10VI GCNNNNNNNGC 9 cut(s) 330, 506, 515, 665, 674, 716, 1505, 1553, 2162
HpyF3I CTNAG 5 cut(s) 631, 1400, 1872, 1968, 1979
HpySE526I ACGT 3 cut(s) 422, 1095, 1910
Hsp92I GRCGYC 1 cut(s) 2054
KflI GGGWCCC 1 cut(s) 939
KroI GCCGGC 1 cut(s) 2056
KroNI GCCGGC 1 cut(s) 2058
Ksp22I TGATCA 1 cut(s) 1207
KspAI GTTAAC 2 cut(s) 1567, 1897
Kzo9I GATC 2 cut(s) 1207, 1756
LmnI GCTCC 9 cut(s) 383, 391, 773, 785, 1222, 1508, 1706, 1876, 2143
Lsp1109I GCAGC 8 cut(s) 62, 153, 556, 686, 1300, 1438, 1930, 2047
LweI GCATC 1 cut(s) 1314
MabI ACCWGGT 1 cut(s) 247
MaeI CTAG 3 cut(s) 566, 980, 1310
MaeII ACGT 3 cut(s) 422, 1095, 1910
MaeIII GTNAC 7 cut(s) 557, 879, 899, 1118, 1443, 1672, 1792
MalI GATC 2 cut(s) 1209, 1758
MboI GATC 2 cut(s) 1207, 1756
MboII GAAGA 4 cut(s) 89, 100, 1008, 2237
MfeI CAATTG 2 cut(s) 131, 660
MflI RGATCY 1 cut(s) 1756
MhlI GDGCHC 7 cut(s) 388, 431, 1430, 1596, 1881, 2039, 2205
MlsI TGGCCA 1 cut(s) 38
MluNI TGGCCA 1 cut(s) 38
MmeI TCCRAC 1 cut(s) 751
Mox20I TGGCCA 1 cut(s) 38
MroNI GCCGGC 1 cut(s) 2056
MscI TGGCCA 1 cut(s) 38
MseI TTAA 5 cut(s) 1362, 1458, 1566, 1896, 2067
MslI CAYNNNNRTG 3 cut(s) 909, 1401, 1862
Msp20I TGGCCA 1 cut(s) 38
MspA1I CMGCKG 4 cut(s) 434, 702, 1451, 2090
MspI CCGG 5 cut(s) 381, 1103, 1374, 1678, 2057
MspR9I CCNGG 8 cut(s) 249, 365, 1266, 1296, 1590, 1678, 2050, 2199
MunI CAATTG 2 cut(s) 131, 660
Mva1269I GAATGC 1 cut(s) 1999
MvaI CCWGG 7 cut(s) 249, 365, 1266, 1296, 1590, 2050, 2199
MwoI GCNNNNNNNGC 9 cut(s) 330, 506, 515, 665, 674, 716, 1505, 1553, 2162
NaeI GCCGGC 1 cut(s) 2058
NciI CCSGG 1 cut(s) 1678
NcoI CCATGG 2 cut(s) 409, 755
NdeII GATC 2 cut(s) 1207, 1756
NgoMIV GCCGGC 1 cut(s) 2056
NmuCI GTSAC 5 cut(s) 557, 899, 1443, 1672, 1792
NspI RCATGY 3 cut(s) 1115, 1636, 2138
OliI CACNNNNGTG 1 cut(s) 1401
PagI TCATGA 1 cut(s) 1470
PaqCI CACCTGC 1 cut(s) 887
PasI CCCWGGG 2 cut(s) 1589, 2198
PciI ACATGT 2 cut(s) 1111, 1632
PctI GAATGC 1 cut(s) 1999
PdiI GCCGGC 1 cut(s) 2058
PfeI GAWTC 6 cut(s) 124, 213, 842, 914, 1045, 1346
PflMI CCANNNNNTGG 1 cut(s) 34
PkrI GCNGC 8 cut(s) 52, 168, 571, 676, 1315, 1453, 1945, 2062
PmaCI CACGTG 1 cut(s) 1096
PmlI CACGTG 1 cut(s) 1096
Ppu21I YACGTR 1 cut(s) 1096
PpuMI RGGWCCY 1 cut(s) 939
PscI ACATGT 2 cut(s) 1111, 1632
Psp124BI GAGCTC 4 cut(s) 388, 1430, 1881, 2039
Psp5II RGGWCCY 1 cut(s) 939
Psp6I CCWGG 7 cut(s) 247, 363, 1264, 1294, 1588, 2048, 2197
PspCI CACGTG 1 cut(s) 1096
PspEI GGTNACC 2 cut(s) 899, 1443
PspFI CCCAGC 2 cut(s) 1763, 1983
PspGI CCWGG 7 cut(s) 247, 363, 1264, 1294, 1588, 2048, 2197
PspPI GGNCC 6 cut(s) 722, 751, 939, 1247, 1292, 1805
PspPPI RGGWCCY 1 cut(s) 939
PstI CTGCAG 1 cut(s) 1129
PstNI CAGNNNCTG 2 cut(s) 491, 2275
PsuI RGATCY 1 cut(s) 1756
PvuII CAGCTG 3 cut(s) 434, 702, 1451
RsaI GTAC 2 cut(s) 524, 1378
RsaNI GTAC 2 cut(s) 523, 1377
RseI CAYNNNNRTG 3 cut(s) 909, 1401, 1862
SacI GAGCTC 4 cut(s) 388, 1430, 1881, 2039
SaqAI TTAA 5 cut(s) 1362, 1458, 1566, 1896, 2067
SatI GCNGC 8 cut(s) 51, 167, 570, 675, 1314, 1452, 1944, 2061
Sau3AI GATC 2 cut(s) 1207, 1756
Sau96I GGNCC 6 cut(s) 722, 751, 939, 1247, 1292, 1805
ScrFI CCNGG 8 cut(s) 249, 365, 1266, 1296, 1590, 1678, 2050, 2199
SduI GDGCHC 7 cut(s) 388, 431, 1430, 1596, 1881, 2039, 2205
SexAI ACCWGGT 1 cut(s) 247
SfaNI GCATC 1 cut(s) 1314
SfcI CTRYAG 2 cut(s) 1125, 1170
SinI GGWCC 3 cut(s) 939, 1247, 1292
SmiMI CAYNNNNRTG 3 cut(s) 909, 1401, 1862
SmlI CTYRAG 2 cut(s) 1421, 2030
SmoI CTYRAG 2 cut(s) 1421, 2030
SsiI CCGC 7 cut(s) 719, 987, 1506, 1809, 1929, 2088, 2247
SspMI CTAG 3 cut(s) 566, 980, 1310
SstI GAGCTC 4 cut(s) 388, 1430, 1881, 2039
StyD4I CCNGG 8 cut(s) 247, 363, 1264, 1294, 1588, 1676, 2048, 2197
StyI CCWWGG 2 cut(s) 409, 755
TaaI ACNGT 5 cut(s) 769, 879, 1198, 1792, 2295
TaiI ACGT 3 cut(s) 425, 1098, 1913
TaqI TCGA 1 cut(s) 425
TfiI GAWTC 6 cut(s) 124, 213, 842, 914, 1045, 1346
Tru1I TTAA 5 cut(s) 1362, 1458, 1566, 1896, 2067
Tru9I TTAA 5 cut(s) 1362, 1458, 1566, 1896, 2067
TscAI CASTG 6 cut(s) 586, 1201, 1797, 1867, 2030, 2164
TseFI GTSAC 5 cut(s) 557, 899, 1443, 1672, 1792
TseI GCWGC 8 cut(s) 50, 166, 569, 674, 1313, 1451, 1943, 2060
Tsp45I GTSAC 5 cut(s) 557, 899, 1443, 1672, 1792
TspDTI ATGAA 6 cut(s) 17, 906, 1227, 1533, 1839, 2142
TspGWI ACGGA 1 cut(s) 136
TspRI CASTG 6 cut(s) 586, 1201, 1797, 1867, 2030, 2164
Van91I CCANNNNNTGG 1 cut(s) 34
VpaK11BI GGWCC 3 cut(s) 939, 1247, 1292
XapI RAATTY 3 cut(s) 442, 690, 1961
XceI RCATGY 3 cut(s) 1115, 1636, 2138
XspI CTAG 3 cut(s) 566, 980, 1310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.