Rroxscaffold_3G00256250

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000302
Physical Location & Seq
Forward (+)
50631661 .. 50633181
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00256250.1

Sequence Viewer

Length: 1521 bp
ATGTACCAAGTTTGTTCTGAACAGAGTTGTTCTGAAAGTGACATTCTAGCTGCTATTGACACTGCTATTGATGATGGCGTGGATGTGCTATCCCTCTCACTTGGTGGTGGCTCAGCTACGTTTTATTGGGATTTTATTGCAATCGGTGCATTTGCAGCAATTCAAAAGGGAATTTTTGTCAGCTGTTCGGCTGGAAATGCGGGGCCTGACTACTCCACTACATCAAATGAGGCCCCATGGATTTTGACAGTTGGAGCAAGCTCCACTGACAGAAACATAAGAGCAATAGCACAGCTTGGAAATGGGCAAGAATATGACGGGGAATCAGTATTCCAGCCTAAAGATTTTAGTTCAAAACAGTTACCTCTTGTTTATGCAGGTGACCATAGCCATGATTCATCAGCTCTTTGCGATGTAGGTTCCCTTACAAATGTTGAAGGGAAAGTAGTGGTGTGTGAGCTAGGTGGCGGAATTGGAAGAATTGCAAAAGGGGTAGAAGTGAAAAGAGCTGGTGGTGTTGCCATGATTCTTGTCAACCCAGACTTTGCTGGCTACACCACCATAGCAGACGTTCACGTGCTTCCGGCAACACATGTGAGTTACTCTGCCGGGGTGAGTATCAAAACCTATATAAACTCAACCTCAACTCCTACAGCAACAATCTTGTTCAAAGGCACTGTCATTGGTGATCAGCTTGCTCCGAAGGTCTCTTTCTTTTCATCAAGAGGACCAAGCCTCGCAAGTCCTGGCATTTTGAAACCTGACATAATTGGTCCCGGTGTGAGCATCCTAGCTGCATGGCCTTTTTCTGTGGACAATGCCACTGAATCTAAAGCCACATTTAACATTATTTCCGGTACTTCAATGTCATGCCCTCACCTGAGTGGCATTGTAGCCTTACTCAAGAGCTCACACCCTGACTGGTCACCAGCTGCCATTAAGTCTGCCATCATGACAACTGCTGAAGTAAACAACCTCGCAGGCTCCGCCATAATGGATGAAACACTATTTGCAGCAGACCTCTTTGCCATTGGTGCAGGTCATGTTAACCCTTCAAAAGCAAATGACCCTGGGCTCATCTACGACACGCAACCAGATGACTACATTCCTTACATGTGTGGTTTGAACTACACAGACCAACAGATAGCAATAGTCACCCGGAAATCAGTGAAATGCTCTGAAGTAGGAGCCATACCCGAAGCACAGCTAAATTATCCCACATTTTCTCTCTTTATAGATCCTGCTGGGAAGCCTCAGAGTTACACAAGAACAGTGACGAATGTTGGGCCCGCTAAATCAACTTATGAAATAGCCGTTGTAAGTCCACATAACATAGACATCAGTGTGCAACCTGAGGAGCTCACATTCACAGAGGTTAACCAGACAATGACGTATCATGTGGTGTTTACCGCACAAAAAGGTGCTGCGAAGGATGGTATAAAATTTTCTCAGGGATATCTGAGCTGGGTCTCTCGCCAGCATTCTGTTAGAAGCCCAATATCTGTGATCTTTGACACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

506

Amino Acids

53.3

Weight (kDa)

4.93

Isoelectric Point (pI)

34.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 2 - 324 5.1e-31 Subtilase family
PA PF02225 120 - 206 4.7e-11 PA domain
fn3_6 PF17766 402 - 502 6e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 368
Acc36I ACCTGC 2 cut(s) 368, 1028
AciI CCGC 5 cut(s) 200, 468, 987, 1290, 1410
AclWI GGATC 1 cut(s) 1232
AcsI RAATTY 2 cut(s) 171, 1442
AcuI CTGAAG 2 cut(s) 984, 1200
AcvI CACGTG 1 cut(s) 577
AdeI CACNNNGTG 1 cut(s) 104
AfaI GTAC 2 cut(s) 5, 859
AflIII ACRYGT 2 cut(s) 592, 1113
AgsI TTSAA 8 cut(s) 164, 354, 437, 670, 757, 864, 1056, 1126
AjnI CCWGG 2 cut(s) 745, 1069
AleI CACNNNNGTG 1 cut(s) 882
Alw21I GWGCWC 2 cut(s) 911, 1362
Alw26I GTCTC 2 cut(s) 712, 1474
AlwI GGATC 1 cut(s) 1232
AoxI GGCC 4 cut(s) 203, 231, 800, 1286
ApaI GGGCCC 1 cut(s) 1290
ApeKI GCWGC 6 cut(s) 50, 155, 794, 932, 1013, 1424
ApoI RAATTY 2 cut(s) 171, 1442
ArsI GACNNNNNNTTYG 2 cut(s) 663, 695
AspS9I GGNCC 6 cut(s) 203, 232, 728, 773, 1286, 1287
AsuC2I CCSGG 3 cut(s) 610, 777, 1159
AsuHPI GGTGA 6 cut(s) 392, 625, 698, 869, 918, 1147
AvaII GGWCC 2 cut(s) 728, 773
AxyI CCTNAGG 1 cut(s) 1353
BaeGI GKGCMC 1 cut(s) 1290
BanII GRGCYC 4 cut(s) 911, 1077, 1290, 1362
BbrPI CACGTG 1 cut(s) 577
Bbv12I GWGCWC 2 cut(s) 911, 1362
BbvI GCAGC 6 cut(s) 37, 167, 781, 919, 1025, 1411
BccI CCATC 3 cut(s) 68, 956, 1427
BceAI ACGGC 1 cut(s) 1298
BciT130I CCWGG 2 cut(s) 747, 1071
BclI TGATCA 1 cut(s) 688
BcnI CCSGG 3 cut(s) 610, 777, 1159
BcoDI GTCTC 2 cut(s) 712, 1474
BfaI CTAG 3 cut(s) 47, 461, 791
BfmI CTRYAG 1 cut(s) 651
BfuAI ACCTGC 2 cut(s) 368, 1028
BisI GCNGC 6 cut(s) 51, 156, 795, 933, 1014, 1425
BlpI GCTNAGC 1 cut(s) 112
BlsI GCNGC 6 cut(s) 52, 157, 796, 934, 1015, 1426
Bme1390I CCNGG 5 cut(s) 610, 747, 777, 1071, 1159
Bme18I GGWCC 2 cut(s) 728, 773
BmgT120I GGNCC 6 cut(s) 203, 232, 728, 773, 1286, 1287
BmiI GGNNCC 7 cut(s) 204, 234, 421, 775, 985, 1189, 1288
BmrFI CCNGG 5 cut(s) 610, 747, 777, 1071, 1159
BmsI GCATC 1 cut(s) 795
Bpu1102I GCTNAGC 1 cut(s) 112
BpuEI CTTGAG 1 cut(s) 887
BpuMI CCSGG 3 cut(s) 610, 777, 1159
BsaAI YACGTR 1 cut(s) 577
BsaI GGTCTC 2 cut(s) 712, 1474
BsaJI CCNNGG 4 cut(s) 236, 609, 1069, 1070
BsaWI WCCGGW 1 cut(s) 854
BsaXI ACNNNNNCTCC 2 cut(s) 631, 661
Bse1I ACTGG 1 cut(s) 926
Bse21I CCTNAGG 1 cut(s) 1353
BseBI CCWGG 2 cut(s) 747, 1071
BseDI CCNNGG 4 cut(s) 236, 609, 1069, 1070
BseGI GGATG 4 cut(s) 88, 786, 1003, 1438
BseMII CTCAG 6 cut(s) 126, 872, 1268, 1344, 1451, 1463
BseNI ACTGG 1 cut(s) 926
BseRI GAGGAG 1 cut(s) 1370
BseSI GKGCMC 1 cut(s) 1290
BseXI GCAGC 6 cut(s) 37, 167, 781, 919, 1025, 1411
BseYI CCCAGC 2 cut(s) 1244, 1464
BsgI GTGCAG 1 cut(s) 1056
BshFI GGCC 4 cut(s) 205, 233, 802, 1288
BsiHKAI GWGCWC 2 cut(s) 911, 1362
BsiSI CCGG 5 cut(s) 584, 609, 777, 855, 1159
BslFI GGGAC 1 cut(s) 759
BsmAI GTCTC 2 cut(s) 712, 1474
BsmFI GGGAC 1 cut(s) 759
BsmI GAATGC 1 cut(s) 1480
BsnI GGCC 4 cut(s) 205, 233, 802, 1288
Bso31I GGTCTC 2 cut(s) 712, 1474
Bsp120I GGGCCC 1 cut(s) 1286
Bsp1286I GDGCHC 4 cut(s) 911, 1077, 1290, 1362
Bsp143I GATC 3 cut(s) 688, 1237, 1506
Bsp1720I GCTNAGC 1 cut(s) 112
Bsp19I CCATGG 1 cut(s) 236
BspACI CCGC 5 cut(s) 200, 468, 987, 1290, 1410
BspANI GGCC 4 cut(s) 205, 233, 802, 1288
BspCNI CTCAG 6 cut(s) 125, 873, 1267, 1345, 1452, 1462
BspHI TCATGA 1 cut(s) 951
BspLI GGNNCC 7 cut(s) 204, 234, 421, 775, 985, 1189, 1288
BspMI ACCTGC 2 cut(s) 368, 1028
BspPI GGATC 1 cut(s) 1232
BspTNI GGTCTC 2 cut(s) 712, 1474
BsrI ACTGG 1 cut(s) 926
BssECI CCNNGG 4 cut(s) 236, 609, 1069, 1070
BssMI GATC 3 cut(s) 688, 1237, 1506
BssT1I CCWWGG 1 cut(s) 236
Bst2UI CCWGG 2 cut(s) 747, 1071
Bst4CI ACNGT 4 cut(s) 250, 360, 679, 1273
BstAPI GCANNNNNTGC 1 cut(s) 146
BstBAI YACGTR 1 cut(s) 577
BstC8I GCNNGC 6 cut(s) 259, 550, 696, 982, 1290, 1478
BstDEI CTNAG 6 cut(s) 112, 881, 1254, 1353, 1449, 1460
BstDSI CCRYGG 1 cut(s) 236
BstEII GGTNACC 2 cut(s) 380, 924
BstF5I GGATG 4 cut(s) 88, 786, 1003, 1438
BstKTI GATC 3 cut(s) 691, 1240, 1509
BstMAI GTCTC 2 cut(s) 712, 1474
BstMBI GATC 3 cut(s) 688, 1237, 1506
BstMWI GCNNNNNNNGC 5 cut(s) 146, 155, 197, 986, 1034
BstNI CCWGG 2 cut(s) 747, 1071
BstNSI RCATGY 2 cut(s) 596, 1117
BstPI GGTNACC 2 cut(s) 380, 924
BstSCI CCNGG 5 cut(s) 608, 745, 775, 1069, 1157
BstSFI CTRYAG 1 cut(s) 651
BstSLI GKGCMC 1 cut(s) 1290
BstV1I GCAGC 6 cut(s) 37, 167, 781, 919, 1025, 1411
BstX2I RGATCY 1 cut(s) 1237
BstYI RGATCY 1 cut(s) 1237
Bsu36I CCTNAGG 1 cut(s) 1353
BsuRI GGCC 4 cut(s) 205, 233, 802, 1288
BtgI CCRYGG 1 cut(s) 236
BtgZI GCGATG 1 cut(s) 426
BtsCI GGATG 4 cut(s) 88, 786, 1003, 1438
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 7 cut(s) 60, 264, 675, 822, 1173, 1278, 1348
BveI ACCTGC 2 cut(s) 368, 1028
Cac8I GCNNGC 6 cut(s) 259, 550, 696, 982, 1290, 1478
CciI TCATGA 1 cut(s) 951
Cfr13I GGNCC 6 cut(s) 203, 232, 728, 773, 1286, 1287
Csp6I GTAC 2 cut(s) 4, 858
CviQI GTAC 2 cut(s) 4, 858
DdeI CTNAG 6 cut(s) 112, 881, 1254, 1353, 1449, 1460
DpnI GATC 3 cut(s) 690, 1239, 1508
DpnII GATC 3 cut(s) 688, 1237, 1506
DraIII CACNNNGTG 1 cut(s) 104
EciI GGCGGA 2 cut(s) 483, 976
Ecl136II GAGCTC 2 cut(s) 909, 1360
Eco130I CCWWGG 1 cut(s) 236
Eco24I GRGCYC 4 cut(s) 911, 1077, 1290, 1362
Eco31I GGTCTC 2 cut(s) 712, 1474
Eco32I GATATC 1 cut(s) 1457
Eco47I GGWCC 2 cut(s) 728, 773
Eco53kI GAGCTC 2 cut(s) 909, 1360
Eco57I CTGAAG 2 cut(s) 984, 1200
Eco72I CACGTG 1 cut(s) 577
Eco81I CCTNAGG 1 cut(s) 1353
Eco91I GGTNACC 2 cut(s) 380, 924
EcoICRI GAGCTC 2 cut(s) 909, 1360
EcoO109I RGGNCCY 2 cut(s) 203, 232
EcoO65I GGTNACC 2 cut(s) 380, 924
EcoRII CCWGG 2 cut(s) 745, 1069
EcoRV GATATC 1 cut(s) 1457
EcoT14I CCWWGG 1 cut(s) 236
EcoT38I GRGCYC 4 cut(s) 911, 1077, 1290, 1362
ErhI CCWWGG 1 cut(s) 236
FaqI GGGAC 1 cut(s) 759
FauI CCCGC 2 cut(s) 193, 1297
FbaI TGATCA 1 cut(s) 688
Fnu4HI GCNGC 6 cut(s) 51, 156, 795, 933, 1014, 1425
FokI GGATG 4 cut(s) 95, 773, 1010, 1445
FriOI GRGCYC 4 cut(s) 911, 1077, 1290, 1362
Fsp4HI GCNGC 6 cut(s) 51, 156, 795, 933, 1014, 1425
FspBI CTAG 3 cut(s) 47, 461, 791
GluI GCNGC 6 cut(s) 51, 156, 795, 933, 1014, 1425
GsaI CCCAGC 2 cut(s) 1248, 1468
HaeIII GGCC 4 cut(s) 205, 233, 802, 1288
HapII CCGG 5 cut(s) 584, 609, 777, 855, 1159
HincII GTYRAC 3 cut(s) 535, 1048, 1378
HindII GTYRAC 3 cut(s) 535, 1048, 1378
HinfI GANTC 4 cut(s) 323, 395, 526, 827
HpaI GTTAAC 2 cut(s) 1048, 1378
HpaII CCGG 5 cut(s) 584, 609, 777, 855, 1159
HphI GGTGA 6 cut(s) 392, 625, 698, 869, 918, 1147
Hpy166II GTNNAC 8 cut(s) 535, 574, 814, 970, 1048, 1325, 1378, 1407
Hpy188I TCNGA 6 cut(s) 19, 34, 702, 1180, 1257, 1461
Hpy188III TCNNGA 3 cut(s) 723, 904, 952
Hpy8I GTNNAC 8 cut(s) 535, 574, 814, 970, 1048, 1325, 1378, 1407
HpyAV CCTTC 4 cut(s) 431, 697, 1062, 1423
HpyCH4III ACNGT 4 cut(s) 250, 360, 679, 1273
HpyCH4IV ACGT 4 cut(s) 119, 570, 576, 1391
HpyCH4V TGCA 9 cut(s) 140, 149, 155, 377, 485, 797, 1013, 1037, 1348
HpyF10VI GCNNNNNNNGC 5 cut(s) 146, 155, 197, 986, 1034
HpyF3I CTNAG 6 cut(s) 112, 881, 1254, 1353, 1449, 1460
HpySE526I ACGT 4 cut(s) 119, 570, 576, 1391
Ksp22I TGATCA 1 cut(s) 688
KspAI GTTAAC 2 cut(s) 1048, 1378
Kzo9I GATC 3 cut(s) 688, 1237, 1506
LmnI GCTCC 6 cut(s) 254, 266, 703, 989, 1187, 1357
Lsp1109I GCAGC 6 cut(s) 37, 167, 781, 919, 1025, 1411
LweI GCATC 1 cut(s) 795
MaeI CTAG 3 cut(s) 47, 461, 791
MaeII ACGT 4 cut(s) 119, 570, 576, 1391
MaeIII GTNAC 8 cut(s) 38, 360, 380, 599, 924, 1153, 1259, 1273
MalI GATC 3 cut(s) 690, 1239, 1508
MboI GATC 3 cut(s) 688, 1237, 1506
MboII GAAGA 1 cut(s) 489
MflI RGATCY 1 cut(s) 1237
MhlI GDGCHC 4 cut(s) 911, 1077, 1290, 1362
MluCI AATT 7 cut(s) 159, 171, 471, 480, 768, 1210, 1442
MmeI TCCRAC 1 cut(s) 232
MseI TTAA 5 cut(s) 843, 939, 1047, 1377, 1519
MslI CAYNNNNRTG 3 cut(s) 390, 882, 1343
MspA1I CMGCKG 2 cut(s) 183, 932
MspI CCGG 5 cut(s) 584, 609, 777, 855, 1159
MspR9I CCNGG 5 cut(s) 610, 747, 777, 1071, 1159
Mva1269I GAATGC 1 cut(s) 1480
MvaI CCWGG 2 cut(s) 747, 1071
MwoI GCNNNNNNNGC 5 cut(s) 146, 155, 197, 986, 1034
NciI CCSGG 3 cut(s) 610, 777, 1159
NcoI CCATGG 1 cut(s) 236
NdeII GATC 3 cut(s) 688, 1237, 1506
NlaIV GGNNCC 7 cut(s) 204, 234, 421, 775, 985, 1189, 1288
NmuCI GTSAC 5 cut(s) 38, 380, 924, 1153, 1273
NspI RCATGY 2 cut(s) 596, 1117
OliI CACNNNNGTG 1 cut(s) 882
PagI TCATGA 1 cut(s) 951
PaqCI CACCTGC 1 cut(s) 368
PasI CCCWGGG 1 cut(s) 1070
PciI ACATGT 2 cut(s) 592, 1113
PctI GAATGC 1 cut(s) 1480
PfeI GAWTC 4 cut(s) 323, 395, 526, 827
PkrI GCNGC 6 cut(s) 52, 157, 796, 934, 1015, 1426
PmaCI CACGTG 1 cut(s) 577
PmlI CACGTG 1 cut(s) 577
Ppu21I YACGTR 1 cut(s) 577
PscI ACATGT 2 cut(s) 592, 1113
Psp124BI GAGCTC 2 cut(s) 911, 1362
Psp6I CCWGG 2 cut(s) 745, 1069
PspCI CACGTG 1 cut(s) 577
PspEI GGTNACC 2 cut(s) 380, 924
PspFI CCCAGC 2 cut(s) 1244, 1464
PspGI CCWGG 2 cut(s) 745, 1069
PspN4I GGNNCC 7 cut(s) 204, 234, 421, 775, 985, 1189, 1288
PspOMI GGGCCC 1 cut(s) 1286
PspPI GGNCC 6 cut(s) 203, 232, 728, 773, 1286, 1287
PsuI RGATCY 1 cut(s) 1237
PvuII CAGCTG 2 cut(s) 183, 932
RsaI GTAC 2 cut(s) 5, 859
RsaNI GTAC 2 cut(s) 4, 858
RseI CAYNNNNRTG 3 cut(s) 390, 882, 1343
SacI GAGCTC 2 cut(s) 911, 1362
SaqAI TTAA 5 cut(s) 843, 939, 1047, 1377, 1519
SatI GCNGC 6 cut(s) 51, 156, 795, 933, 1014, 1425
Sau3AI GATC 3 cut(s) 688, 1237, 1506
Sau96I GGNCC 6 cut(s) 203, 232, 728, 773, 1286, 1287
ScrFI CCNGG 5 cut(s) 610, 747, 777, 1071, 1159
SduI GDGCHC 4 cut(s) 911, 1077, 1290, 1362
SfaNI GCATC 1 cut(s) 795
SfcI CTRYAG 1 cut(s) 651
SinI GGWCC 2 cut(s) 728, 773
SmiMI CAYNNNNRTG 3 cut(s) 390, 882, 1343
SmlI CTYRAG 1 cut(s) 902
SmoI CTYRAG 1 cut(s) 902
Sse9I AATT 7 cut(s) 159, 171, 471, 480, 768, 1210, 1442
SsiI CCGC 5 cut(s) 200, 468, 987, 1290, 1410
SspMI CTAG 3 cut(s) 47, 461, 791
SstI GAGCTC 2 cut(s) 911, 1362
StyD4I CCNGG 5 cut(s) 608, 745, 775, 1069, 1157
StyI CCWWGG 1 cut(s) 236
TaaI ACNGT 4 cut(s) 250, 360, 679, 1273
TaiI ACGT 4 cut(s) 122, 573, 579, 1394
TasI AATT 7 cut(s) 159, 171, 471, 480, 768, 1210, 1442
TfiI GAWTC 4 cut(s) 323, 395, 526, 827
Tru1I TTAA 5 cut(s) 843, 939, 1047, 1377, 1519
Tru9I TTAA 5 cut(s) 843, 939, 1047, 1377, 1519
TscAI CASTG 7 cut(s) 67, 271, 682, 829, 1173, 1278, 1348
TseFI GTSAC 5 cut(s) 38, 380, 924, 1153, 1273
TseI GCWGC 6 cut(s) 50, 155, 794, 932, 1013, 1424
Tsp45I GTSAC 5 cut(s) 38, 380, 924, 1153, 1273
TspDTI ATGAA 4 cut(s) 387, 708, 1014, 1320
TspRI CASTG 7 cut(s) 67, 271, 682, 829, 1173, 1278, 1348
VpaK11BI GGWCC 2 cut(s) 728, 773
XapI RAATTY 2 cut(s) 171, 1442
XceI RCATGY 2 cut(s) 596, 1117
XspI CTAG 3 cut(s) 47, 461, 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.