Prupe.5G085200_v2.0.a1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000302
Physical Location & Seq
Forward (+)
9726784 .. 9729120
2337 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G085200.1

Sequence Viewer

Length: 2337 bp
ATGGAGAACAAAAAGGGTGCATCATTAATGCAGGTTATATATCTTGTTGGTTTAGTTTTCATGTTTTGTTTATCACTAACAGTTGCGGATTATGAAGAAATGGCTCTGGAATCGGTAGTTGCTGAGAAAAATGATGGCAACAATGACATGCAAACTTATATTGTTTGGGTGCAAAAGCCAGCCTCTCAGAAATTTTCTGCAGAATCTCATGAAGATTTGGAGAGTTGGTATCAGTCATTTTTGCCTGAAACTGATGCAAACTCAAACCAGCTGACGAAACAACGGATGGTTTATGCATACCACAATGTGGCAACAGGGTTTGCAGCAAGACTGACACCAGAGGAAGTAAAAACAATGGAAACGATAGAAGGGTTTGTGTCAGCTCGTCCAGAGAAGATTCTGCCGTTGCAGACTACTCATAGTCCCGACTTCTTGGGTTTGCACCAAGGATTTGGACTTTGGGAAGCAACAAACTATGGTGAAGGTGTGATAATTGGACTTTTGGATACTGGGATTACACCTGATCATCCCTCATTTAGTGATGAAGGAATGCCACCTCCTCCTGCTAAATGGAAAGGTAAGTGTGAGTTCAATGGGACCTTGTGCAATAACAAGCTTATCGGTGCAAGAAATTTCAAGGGAGTAGGCAAAGATCAACCCCCACCAGGTCCTCCATTTGACATAGATGGCCATGGCACCCACACATCTAGCACTGCTGCTGGAAGGTTTGTGGAAGGCGCAAGCGTGTTTGGACAGGCCAATGGCACAGCGGCTGGCATGGCACCGAAAGCTCACTTGGCAGTTTACAAAGTCTGCTCTGGCTTTGGTTGTGCTGAAGCTGACATTTTGGCTGCTCTTGATGTTGCTGTTGATGATGGCGTGGACGTGCTCTCCCTCTCACTTGGAGGCCCCTCACTTCCTTTCTATAAGGATGTGATTGCAATTGGTGCATTTGCAGCAATTCAAAAGGGAATTTTTTTCAGCTGTGCAGGTGGAAATTTTGGTCCTTTCAACCGTTCTTTATCAAACGAGGCCCCGTGGATTCTGACAGTTGGAGCAAGCACTACCGACAGAATATTAAAATCAGAAGCGCAGATCGGGGATGATAAAAATAACCATTTTGATGGAAAATCTTTGTCCCAGCCTCAAGATTTCGATTCAACAGTTTCGTTACCTTTACCTCTTGTTTATGCAGGCTCAGTTGGCAACCAGCCATCAGCTTTCTGCAAGAAAGGGACTCTCGAAAACGTTGAAGGGAAAATAGTACTGTGTGAGACAGGTGGAGGAGTTACAAATATTGCCAAAGGGGAAGAAGTGAAACGAGCTGGTGGTGCTGCCATGATTCTCATGAACCAAGAAACTGATGGCTTTAGCACCTTAGCTGAATCACATGCGCTACCCACGACACATGTGAGTTATGCTGCAGGGTTGCAAATCAAGTCATATATAAGCTCAACCTCAAGTCCTACAGCCACAATCTTGTTCAACGGCACTGTCATTGGAGACGCACTTGCTCCCAGAGTTGCTTTCTTTTCATCAAGAGGACCAAACAAGGCAAGCCCCGGAATTTTGAAACCTGACATCATTGGACCCGGTGTTGACATCCTAGCAGCATGGCCTGTTTCTGTGGACAATGCCACGCAGTCTAAGGCAACATTTAACATAATTTCTGGTACTTCAATGGCTTGTCCTCACCTAAGTGGAATTGCAGCCTTGGTCAAGAGCTCACACCCTGATTGGTCACCAGCTGCAATTAAATCTGCCATCATGACAACCGCTGATGTGCTAAACCTTGCAGGCTTGCCCATTGTTGATCAAGACCAAAGGCCTGCGAATGTCTTTGCAATCGGTGCAGGCCATGTTAATCCTTCAAAAGCAAATGACCCGGGGCTCATCTACGACACACAACCAGAGGATTACATTCCTTACTTGTGTGGTTTGAATTACACTGATAAAGAGATACAGATCATCACCCAACAAAAAGTGAACTGCTCTCAAGTCGGGGTCATATCAGAAGCACAGCTGAATTATCCTTCATTTTCTATTAAGATAGGGTCCAATGAGTCTCAGTCTCAGTATTATACAAGGACAGTGAGGAATGTTGGCCCGGCTAGTACAACTTACAACTTGGATCTTCTTGTACCACATAAAATGGGCATGAGTGTGAATCCTGGGGTTCTTACATTTACTGAGGTCAACCAGGAGATTACATTCCATGTGGAGTTTATAGCACAAGATGGTGCTGGGAAGGATGGTGTAGCATTTGGTCAGGGATATTTGAGATGGGTTTCTGATAAGCATAATGTTACTAGCCCAATAGCTGTGGTCTTCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

779

Amino Acids

82.77

Weight (kDa)

5.11

Isoelectric Point (pI)

36.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 980
Acc36I ACCTGC 2 cut(s) 22, 980
AccB1I GGYRCC 2 cut(s) 695, 781
AccB7I CCANNNNNTGG 1 cut(s) 307
AciI CCGC 3 cut(s) 86, 770, 1776
AclI AACGTT 1 cut(s) 1248
AclWI GGATC 1 cut(s) 2139
AcoI YGGCCR 1 cut(s) 688
AcsI RAATTY 5 cut(s) 191, 631, 972, 997, 1566
AcuI CTGAAG 1 cut(s) 855
AdeI CACNNNGTG 1 cut(s) 307
AfaI GTAC 4 cut(s) 1266, 1675, 2116, 2142
AfiI CCNNNNNNNGG 2 cut(s) 307, 665
AflIII ACRYGT 1 cut(s) 1408
AjiI CACGTC 1 cut(s) 886
AjnI CCWGG 3 cut(s) 664, 2170, 2199
AjuI GAANNNNNNNTTGG 2 cut(s) 779, 811
AleI CACNNNNGTG 1 cut(s) 1698
Alw21I GWGCWC 2 cut(s) 891, 1727
Alw26I GTCTC 4 cut(s) 1268, 1497, 2070, 2076
AlwI GGATC 1 cut(s) 2139
AlwNI CAGNNNCTG 1 cut(s) 773
Ama87I CYCGRG 1 cut(s) 1885
AoxI GGCC 8 cut(s) 688, 756, 907, 1032, 1616, 1826, 1855, 2104
ApeKI GCWGC 9 cut(s) 323, 716, 851, 956, 1334, 1421, 1610, 1709, 1748
ApoI RAATTY 5 cut(s) 191, 631, 972, 997, 1566
AseI ATTAAT 1 cut(s) 26
AspLEI GCGC 3 cut(s) 740, 1093, 1396
AspS9I GGNCC 9 cut(s) 597, 668, 908, 1004, 1033, 1544, 1589, 2055, 2105
AsuC2I CCSGG 5 cut(s) 1563, 1593, 1886, 1887, 2108
AsuHPI GGTGA 4 cut(s) 491, 1685, 1734, 1963
AvaI CYCGRG 1 cut(s) 1885
AvaII GGWCC 6 cut(s) 597, 668, 1004, 1544, 1589, 2055
BalI TGGCCA 1 cut(s) 690
BanI GGYRCC 2 cut(s) 695, 781
BanII GRGCYC 2 cut(s) 1727, 1893
BbsI GAAGAC 1 cut(s) 2320
Bbv12I GWGCWC 2 cut(s) 891, 1727
BbvI GCAGC 9 cut(s) 335, 703, 838, 968, 1321, 1408, 1622, 1721, 1735
BceAI ACGGC 2 cut(s) 388, 1504
BciT130I CCWGG 3 cut(s) 666, 2172, 2201
BciVI GTATCC 1 cut(s) 499
BclI TGATCA 2 cut(s) 523, 1813
BcnI CCSGG 5 cut(s) 1563, 1593, 1886, 1887, 2108
BcoDI GTCTC 4 cut(s) 1268, 1497, 2070, 2076
BfaI CTAG 4 cut(s) 708, 1607, 2112, 2310
BfmI CTRYAG 3 cut(s) 198, 1422, 1467
BfuAI ACCTGC 2 cut(s) 22, 980
BfuI GTATCC 1 cut(s) 499
BmcAI AGTACT 1 cut(s) 1266
Bme1390I CCNGG 8 cut(s) 666, 1563, 1593, 1886, 1887, 2108, 2172, 2201
Bme18I GGWCC 6 cut(s) 597, 668, 1004, 1544, 1589, 2055
BmeT110I CYCGRG 1 cut(s) 1885
BmgBI CACGTC 1 cut(s) 886
BmgT120I GGNCC 9 cut(s) 597, 668, 908, 1004, 1033, 1544, 1589, 2055, 2105
BmiI GGNNCC 7 cut(s) 598, 697, 783, 910, 1035, 1591, 2056
BmrFI CCNGG 8 cut(s) 666, 1563, 1593, 1886, 1887, 2108, 2172, 2201
BmrI ACTGGG 1 cut(s) 519
BmsI GCATC 2 cut(s) 29, 244
BmuI ACTGGG 1 cut(s) 519
BpiI GAAGAC 1 cut(s) 2320
Bpu10I CCTNAGC 1 cut(s) 1378
BpuEI CTTGAG 3 cut(s) 1131, 1444, 1980
BpuMI CCSGG 5 cut(s) 1563, 1593, 1886, 1887, 2108
BsaBI GATNNNNATC 1 cut(s) 1964
BsaJI CCNNGG 8 cut(s) 445, 691, 1037, 1561, 1713, 1885, 1886, 2171
BsaXI ACNNNNNCTCC 4 cut(s) 212, 242, 875, 905
Bsc4I CCNNNNNNNGG 2 cut(s) 307, 665
Bse1I ACTGG 1 cut(s) 514
Bse8I GATNNNNATC 1 cut(s) 1964
BseBI CCWGG 3 cut(s) 666, 2172, 2201
BseDI CCNNGG 8 cut(s) 445, 691, 1037, 1561, 1713, 1885, 1886, 2171
BseGI GGATG 6 cut(s) 291, 526, 937, 1108, 1602, 2257
BseJI GATNNNNATC 1 cut(s) 1964
BseLI CCNNNNNNNGG 2 cut(s) 307, 665
BseMII CTCAG 6 cut(s) 114, 200, 1212, 2081, 2087, 2181
BseNI ACTGG 1 cut(s) 514
BseRI GAGGAG 2 cut(s) 549, 1299
BseXI GCAGC 9 cut(s) 335, 703, 838, 968, 1321, 1408, 1622, 1721, 1735
BseYI CCCAGC 2 cut(s) 1140, 2243
BsgI GTGCAG 2 cut(s) 1008, 1872
BshFI GGCC 8 cut(s) 690, 758, 909, 1034, 1618, 1828, 1857, 2106
BshNI GGYRCC 2 cut(s) 695, 781
BsiHKAI GWGCWC 2 cut(s) 891, 1727
BsiHKCI CYCGRG 1 cut(s) 1885
BsiSI CCGG 4 cut(s) 1563, 1593, 1886, 2108
BslFI GGGAC 4 cut(s) 408, 610, 1123, 1249
BslI CCNNNNNNNGG 2 cut(s) 307, 665
BsmAI GTCTC 4 cut(s) 1268, 1497, 2070, 2076
BsmBI CGTCTC 1 cut(s) 1497
BsmFI GGGAC 4 cut(s) 408, 610, 1123, 1249
BsmI GAATGC 1 cut(s) 555
BsnI GGCC 8 cut(s) 690, 758, 909, 1034, 1618, 1828, 1857, 2106
BsoBI CYCGRG 1 cut(s) 1885
Bsp1286I GDGCHC 3 cut(s) 891, 1727, 1893
Bsp143I GATC 6 cut(s) 523, 652, 1095, 1813, 1965, 2131
Bsp19I CCATGG 1 cut(s) 691
BspACI CCGC 3 cut(s) 86, 770, 1776
BspANI GGCC 8 cut(s) 690, 758, 909, 1034, 1618, 1828, 1857, 2106
BspCNI CTCAG 6 cut(s) 115, 199, 1211, 2080, 2086, 2182
BspHI TCATGA 3 cut(s) 208, 1347, 1767
BspLI GGNNCC 7 cut(s) 598, 697, 783, 910, 1035, 1591, 2056
BspMAI CTGCAG 2 cut(s) 202, 1426
BspMI ACCTGC 2 cut(s) 22, 980
BspPI GGATC 1 cut(s) 2139
BspT107I GGYRCC 2 cut(s) 695, 781
BsrI ACTGG 1 cut(s) 514
BssECI CCNNGG 8 cut(s) 445, 691, 1037, 1561, 1713, 1885, 1886, 2171
BssMI GATC 6 cut(s) 523, 652, 1095, 1813, 1965, 2131
BssT1I CCWWGG 3 cut(s) 445, 691, 1713
Bst2UI CCWGG 3 cut(s) 666, 2172, 2201
Bst4CI ACNGT 7 cut(s) 82, 1016, 1051, 1165, 1269, 1495, 2092
BstAPI GCANNNNNTGC 2 cut(s) 947, 1850
BstDEI CTNAG 9 cut(s) 123, 186, 1198, 1378, 1647, 1697, 2067, 2073, 2190
BstDSI CCRYGG 2 cut(s) 691, 1037
BstEII GGTNACC 1 cut(s) 1740
BstF5I GGATG 6 cut(s) 291, 526, 937, 1108, 1602, 2257
BstHHI GCGC 3 cut(s) 740, 1093, 1396
BstKTI GATC 6 cut(s) 526, 655, 1098, 1816, 1968, 2134
BstMAI GTCTC 4 cut(s) 1268, 1497, 2070, 2076
BstMBI GATC 6 cut(s) 523, 652, 1095, 1813, 1965, 2131
BstMWI GCNNNNNNNGC 8 cut(s) 779, 788, 797, 947, 956, 1203, 1331, 1850
BstNI CCWGG 3 cut(s) 666, 2172, 2201
BstNSI RCATGY 3 cut(s) 151, 1394, 1412
BstPI GGTNACC 1 cut(s) 1740
BstSCI CCNGG 8 cut(s) 664, 1561, 1591, 1884, 1885, 2106, 2170, 2199
BstSFI CTRYAG 3 cut(s) 198, 1422, 1467
BstV1I GCAGC 9 cut(s) 335, 703, 838, 968, 1321, 1408, 1622, 1721, 1735
BstV2I GAAGAC 1 cut(s) 2320
BstX2I RGATCY 1 cut(s) 2131
BstXI CCANNNNNNTGG 2 cut(s) 452, 1124
BstYI RGATCY 1 cut(s) 2131
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 8 cut(s) 690, 758, 909, 1034, 1618, 1828, 1857, 2106
BtgI CCRYGG 2 cut(s) 691, 1037
BtrI CACGTC 1 cut(s) 886
BtsCI GGATG 6 cut(s) 291, 526, 937, 1108, 1602, 2257
BtsI GCAGTG 1 cut(s) 711
BtsIMutI CAGTG 4 cut(s) 711, 1491, 1947, 2097
BveI ACCTGC 2 cut(s) 22, 980
CaiI CAGNNNCTG 1 cut(s) 773
CciI TCATGA 3 cut(s) 208, 1347, 1767
CfoI GCGC 3 cut(s) 740, 1093, 1396
Cfr13I GGNCC 9 cut(s) 597, 668, 908, 1004, 1033, 1544, 1589, 2055, 2105
Cfr9I CCCGGG 1 cut(s) 1885
CseI GACGC 1 cut(s) 1514
CsiI ACCWGGT 1 cut(s) 664
Csp6I GTAC 4 cut(s) 1265, 1674, 2115, 2141
CspCI CAANNNNNGTGG 1 cut(s) 2304
CviQI GTAC 4 cut(s) 1265, 1674, 2115, 2141
DdeI CTNAG 9 cut(s) 123, 186, 1198, 1378, 1647, 1697, 2067, 2073, 2190
DpnI GATC 6 cut(s) 525, 654, 1097, 1815, 1967, 2133
DpnII GATC 6 cut(s) 523, 652, 1095, 1813, 1965, 2131
DraIII CACNNNGTG 1 cut(s) 307
EaeI YGGCCR 1 cut(s) 688
Ecl136II GAGCTC 1 cut(s) 1725
Eco130I CCWWGG 3 cut(s) 445, 691, 1713
Eco147I AGGCCT 1 cut(s) 1828
Eco24I GRGCYC 2 cut(s) 1727, 1893
Eco47I GGWCC 6 cut(s) 597, 668, 1004, 1544, 1589, 2055
Eco53kI GAGCTC 1 cut(s) 1725
Eco57I CTGAAG 1 cut(s) 855
Eco88I CYCGRG 1 cut(s) 1885
Eco91I GGTNACC 1 cut(s) 1740
EcoICRI GAGCTC 1 cut(s) 1725
EcoO109I RGGNCCY 4 cut(s) 597, 668, 908, 1033
EcoO65I GGTNACC 1 cut(s) 1740
EcoRII CCWGG 3 cut(s) 664, 2170, 2199
EcoT14I CCWWGG 3 cut(s) 445, 691, 1713
EcoT22I ATGCAT 1 cut(s) 298
EcoT38I GRGCYC 2 cut(s) 1727, 1893
ErhI CCWWGG 3 cut(s) 445, 691, 1713
Esp3I CGTCTC 1 cut(s) 1497
FaqI GGGAC 4 cut(s) 408, 610, 1123, 1249
FbaI TGATCA 2 cut(s) 523, 1813
FokI GGATG 6 cut(s) 298, 513, 944, 1115, 1589, 2264
FriOI GRGCYC 2 cut(s) 1727, 1893
FspBI CTAG 4 cut(s) 708, 1607, 2112, 2310
GlaI GCGC 3 cut(s) 739, 1092, 1395
GsaI CCCAGC 2 cut(s) 1144, 2247
HaeIII GGCC 8 cut(s) 690, 758, 909, 1034, 1618, 1828, 1857, 2106
HapII CCGG 4 cut(s) 1563, 1593, 1886, 2108
HgaI GACGC 1 cut(s) 1514
HhaI GCGC 3 cut(s) 740, 1093, 1396
Hin6I GCGC 3 cut(s) 738, 1091, 1394
HinP1I GCGC 3 cut(s) 738, 1091, 1394
HincII GTYRAC 2 cut(s) 1600, 2197
HindII GTYRAC 2 cut(s) 1600, 2197
HindIII AAGCTT 1 cut(s) 614
HpaII CCGG 4 cut(s) 1563, 1593, 1886, 2108
HphI GGTGA 4 cut(s) 491, 1685, 1734, 1963
Hpy166II GTNNAC 6 cut(s) 805, 883, 1600, 1630, 1987, 2197
Hpy188I TCNGA 5 cut(s) 189, 1047, 1087, 2014, 2293
Hpy8I GTNNAC 6 cut(s) 805, 883, 1600, 1630, 1987, 2197
HpyAV CCTTC 9 cut(s) 362, 476, 539, 717, 728, 1247, 1878, 2043, 2242
HpyCH4III ACNGT 7 cut(s) 82, 1016, 1051, 1165, 1269, 1495, 2092
HpyCH4IV ACGT 2 cut(s) 885, 1248
HpyF10VI GCNNNNNNNGC 8 cut(s) 779, 788, 797, 947, 956, 1203, 1331, 1850
HpyF3I CTNAG 9 cut(s) 123, 186, 1198, 1378, 1647, 1697, 2067, 2073, 2190
HpySE526I ACGT 2 cut(s) 885, 1248
HspAI GCGC 3 cut(s) 738, 1091, 1394
Ksp22I TGATCA 2 cut(s) 523, 1813
Kzo9I GATC 6 cut(s) 523, 652, 1095, 1813, 1965, 2131
LmnI GCTCC 2 cut(s) 1055, 1519
Lsp1109I GCAGC 9 cut(s) 335, 703, 838, 968, 1321, 1408, 1622, 1721, 1735
LweI GCATC 2 cut(s) 29, 244
MabI ACCWGGT 1 cut(s) 664
MaeI CTAG 4 cut(s) 708, 1607, 2112, 2310
MaeII ACGT 2 cut(s) 885, 1248
MaeIII GTNAC 4 cut(s) 1170, 1288, 1740, 2305
MalI GATC 6 cut(s) 525, 654, 1097, 1815, 1967, 2133
MboI GATC 6 cut(s) 523, 652, 1095, 1813, 1965, 2131
MboII GAAGA 6 cut(s) 107, 224, 406, 1322, 2126, 2320
MfeI CAATTG 1 cut(s) 942
MflI RGATCY 1 cut(s) 2131
MhlI GDGCHC 3 cut(s) 891, 1727, 1893
MlsI TGGCCA 1 cut(s) 690
MluNI TGGCCA 1 cut(s) 690
MlyI GAGTC 2 cut(s) 1231, 2072
MmeI TCCRAC 1 cut(s) 1033
Mox20I TGGCCA 1 cut(s) 690
Mph1103I ATGCAT 1 cut(s) 298
MscI TGGCCA 1 cut(s) 690
MseI TTAA 6 cut(s) 26, 1079, 1659, 1755, 1863, 2046
MslI CAYNNNNRTG 3 cut(s) 1122, 1698, 2162
Msp20I TGGCCA 1 cut(s) 690
MspA1I CMGCKG 6 cut(s) 271, 770, 984, 1748, 1778, 2023
MspI CCGG 4 cut(s) 1563, 1593, 1886, 2108
MspR9I CCNGG 8 cut(s) 666, 1563, 1593, 1886, 1887, 2108, 2172, 2201
MunI CAATTG 1 cut(s) 942
Mva1269I GAATGC 1 cut(s) 555
MvaI CCWGG 3 cut(s) 666, 2172, 2201
MwoI GCNNNNNNNGC 8 cut(s) 779, 788, 797, 947, 956, 1203, 1331, 1850
NciI CCSGG 5 cut(s) 1563, 1593, 1886, 1887, 2108
NcoI CCATGG 1 cut(s) 691
NdeII GATC 6 cut(s) 523, 652, 1095, 1813, 1965, 2131
NlaIV GGNNCC 7 cut(s) 598, 697, 783, 910, 1035, 1591, 2056
NmuCI GTSAC 1 cut(s) 1740
NsiI ATGCAT 1 cut(s) 298
NspI RCATGY 3 cut(s) 151, 1394, 1412
OliI CACNNNNGTG 1 cut(s) 1698
PagI TCATGA 3 cut(s) 208, 1347, 1767
PaqCI CACCTGC 1 cut(s) 980
PceI AGGCCT 1 cut(s) 1828
PciI ACATGT 1 cut(s) 1408
PctI GAATGC 1 cut(s) 555
PfeI GAWTC 8 cut(s) 110, 203, 397, 1042, 1157, 1342, 1385, 2167
PflMI CCANNNNNTGG 1 cut(s) 307
PleI GAGTC 2 cut(s) 1231, 2071
PpsI GAGTC 2 cut(s) 1231, 2071
PpuMI RGGWCCY 2 cut(s) 597, 668
PscI ACATGT 1 cut(s) 1408
PshBI ATTAAT 1 cut(s) 26
Psp124BI GAGCTC 1 cut(s) 1727
Psp1406I AACGTT 1 cut(s) 1248
Psp5II RGGWCCY 2 cut(s) 597, 668
Psp6I CCWGG 3 cut(s) 664, 2170, 2199
PspEI GGTNACC 1 cut(s) 1740
PspFI CCCAGC 2 cut(s) 1140, 2243
PspGI CCWGG 3 cut(s) 664, 2170, 2199
PspN4I GGNNCC 7 cut(s) 598, 697, 783, 910, 1035, 1591, 2056
PspPI GGNCC 9 cut(s) 597, 668, 908, 1004, 1033, 1544, 1589, 2055, 2105
PspPPI RGGWCCY 2 cut(s) 597, 668
PstI CTGCAG 2 cut(s) 202, 1426
PstNI CAGNNNCTG 1 cut(s) 773
PsuI RGATCY 1 cut(s) 2131
PvuII CAGCTG 4 cut(s) 271, 984, 1748, 2023
RsaI GTAC 4 cut(s) 1266, 1675, 2116, 2142
RsaNI GTAC 4 cut(s) 1265, 1674, 2115, 2141
RseI CAYNNNNRTG 3 cut(s) 1122, 1698, 2162
SacI GAGCTC 1 cut(s) 1727
SaqAI TTAA 6 cut(s) 26, 1079, 1659, 1755, 1863, 2046
Sau3AI GATC 6 cut(s) 523, 652, 1095, 1813, 1965, 2131
Sau96I GGNCC 9 cut(s) 597, 668, 908, 1004, 1033, 1544, 1589, 2055, 2105
ScaI AGTACT 1 cut(s) 1266
SchI GAGTC 2 cut(s) 1231, 2072
ScrFI CCNGG 8 cut(s) 666, 1563, 1593, 1886, 1887, 2108, 2172, 2201
SduI GDGCHC 3 cut(s) 891, 1727, 1893
SexAI ACCWGGT 1 cut(s) 664
SfaNI GCATC 2 cut(s) 29, 244
SfcI CTRYAG 3 cut(s) 198, 1422, 1467
SinI GGWCC 6 cut(s) 597, 668, 1004, 1544, 1589, 2055
SmaI CCCGGG 1 cut(s) 1887
SmiMI CAYNNNNRTG 3 cut(s) 1122, 1698, 2162
SmlI CTYRAG 3 cut(s) 1146, 1459, 1995
SmoI CTYRAG 3 cut(s) 1146, 1459, 1995
SseBI AGGCCT 1 cut(s) 1828
SsiI CCGC 3 cut(s) 86, 770, 1776
SspI AATATT 2 cut(s) 1077, 1297
SspMI CTAG 4 cut(s) 708, 1607, 2112, 2310
SstI GAGCTC 1 cut(s) 1727
StuI AGGCCT 1 cut(s) 1828
StyD4I CCNGG 8 cut(s) 664, 1561, 1591, 1884, 1885, 2106, 2170, 2199
StyI CCWWGG 3 cut(s) 445, 691, 1713
TaaI ACNGT 7 cut(s) 82, 1016, 1051, 1165, 1269, 1495, 2092
TaiI ACGT 2 cut(s) 888, 1251
TaqI TCGA 3 cut(s) 1155, 1242, 2331
TatI WGTACW 2 cut(s) 1264, 2114
TauI GCSGC 1 cut(s) 773
TfiI GAWTC 8 cut(s) 110, 203, 397, 1042, 1157, 1342, 1385, 2167
Tru1I TTAA 6 cut(s) 26, 1079, 1659, 1755, 1863, 2046
Tru9I TTAA 6 cut(s) 26, 1079, 1659, 1755, 1863, 2046
TscAI CASTG 4 cut(s) 718, 1498, 1954, 2097
TseFI GTSAC 1 cut(s) 1740
TseI GCWGC 9 cut(s) 323, 716, 851, 956, 1334, 1421, 1610, 1709, 1748
Tsp45I GTSAC 1 cut(s) 1740
TspDTI ATGAA 7 cut(s) 49, 108, 225, 558, 1364, 1524, 2025
TspGWI ACGGA 1 cut(s) 298
TspMI CCCGGG 1 cut(s) 1885
TspRI CASTG 4 cut(s) 718, 1498, 1954, 2097
Van91I CCANNNNNTGG 1 cut(s) 307
VpaK11BI GGWCC 6 cut(s) 597, 668, 1004, 1544, 1589, 2055
VspI ATTAAT 1 cut(s) 26
XapI RAATTY 5 cut(s) 191, 631, 972, 997, 1566
XceI RCATGY 3 cut(s) 151, 1394, 1412
XcmI CCANNNNNNNNNTGG 1 cut(s) 1361
XmaI CCCGGG 1 cut(s) 1885
XspI CTAG 4 cut(s) 708, 1607, 2112, 2310
ZrmI AGTACT 1 cut(s) 1266
Zsp2I ATGCAT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.