RH7DG187900

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0000302
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG187900.1

Sequence Viewer

Length: 2298 bp
ATGGAGAACAAAAAGTGTGTAGTGATGCAGATCATATACCTTCTTGGCTTTAGTTGCATGTTTTACTTGTCACTAGCAGTTGCTGTCGAAAAAGTGGCAGTACTGGAACCACAAGGTGAGACAGATAGCATGCAAATTTATATAGTTTTGGTTGAAAGGCCACCCAGCAATTTCAATGCACAATCTGATCATGAAGATTTAGAGAGTTGGTACCAGTCATTTCTGCCCCAAACTATTGCAAACTCAAACCAGCTCATGAAGCCACGAATGGTTTATGCATACCACAATGTGGCCACAGGATTTGCAGCAAAGCTGACTGGGGAGGAAGTTGAAGAAATGGAGAAGAAAGATGGGTTTATCTACGCTCATCCAGAACGGAATCTGCAATTGCACACAACTCATAGTCCTAACTTCTTGGGGCTGCAACAAGGATTGGGACTTTGGAAAGGGTCTAATTATGGTGCAGGAATCATTATTGGAGTCTTGGATACTGGAATAACACCAGGTCATCCCTCATTCAGCGACGAAGGAGTGCCACCTCCTCCGGCTAAATGGAAAGGCAAGTGTGATTTCAATGGCACATTCTGCAACAACAAGCTTATTGGTGCCAGAAGTTTCCAGGGTGGGAACACTACCGGAGCTCCACCAGTTGACATTGCAGGCCATGGAACCCACACGTCGAGCACAGCTGGTGGAAATTTTGTGAAAGGTGCCAACGTGTTTGGAATGGCAAATGGAACAGCCTCTGGCATGGCACCTTATGCTCACTTGGCAATGTACCAAGTTTGTTCTGAACAGGGTTGTTCTGAAAGTGACATTCTAGCTGCTATTGACACTGCTATTGATGATGGCGTGGATGTGCTATCCATCTCACTTGGTGGTGGCTCAGCTACTTTTTATTGGGATTTTATTGCAATTGGTGCATTTGCAGCAATTCAGAAGGGAATTTTTGTCAGCTGTTCGGCTGGAAATGCAGGGCCTGACTACTCTACTACATCAAATGAGGCCCCATGGATTTTGACAGTTGGAGCAAGCTCCATTGACAGAAACATAAGAGCAATAGCACAGCTTGGAAATGGGCAAGAATATGACGGGGAATCAGTATTCCAGCCTAAAGATTTTAGTTCAAAACAGTTACCTCTTGTTTATGCAGGTGACCATAGCCATGATTCATCAGCTCTTTGCGATGTAGGGTCCCTTACAAATGTTGAAGGGAAAGTAGTGGTGTGTGAGCTAGGTGGAGGAATTGGAAGAATTGCCAAAGGAGAAGAAGTCAAAAGAGCAGGTGGTGCTGCCATGATCCTCATGAACGAAGACACTGATGGCTATAGCACTTTAGCTGATGCTCATGTGCTTCCTGCAACACATGTGAGTTATGCTGCAGGTTTGAGCATCAAAGCCTACATAAACTCCACCTCAACACCTATGGCCACAATCTTGTTCAAAGGCACTGTCATCGGTGATCAAAATGCTCCCGCAGTCACTTCCTTTTCATCAAGAGGACCAAGCCTGGCGAGCACAGGAATCTTGAAACCTGACATCATTGGTCCCGGTGTTAGCATCTTAGCTGCATGGCCTGTTTCAGTGGACAATGGCACAGAATCTACGGCAACATTTAACATGGTTTCAGGTACTTCAATGTCATGCCCTCACCTGAGTGGCATTGCAGCTTTGCTCAAGAGCTCACACCCCGACTGGTCACCAGCTGCCATCAAGTCTGCAATTGTGACAACCGCTGACATAGTAAACCTCGGAGGCAAACCTATTATTGATGAAACACTTGCTCCAGCAGACATCTTTGCCACTGGTGCAGGACATGTTAATCCTCCAAAAGCAGATGACCCTGGGCTAATCTACGACACAAAACCAACGGATTACATTCCCTACCTGTGCGGTTTGAACTACACAGACGATCAGATACAGATAATCACCCAACAAACAGTGAAGTGCTCTGAAGTAGGAGTCATACCTGAAGCGCAGCTAAATTATCCTTCATTTTCTATTATATTCCAGGACTTGTCTCAGTCTCAAGATTACACAAGGACGGTGACAAATGTTGGACCAGCTAATTCAACTTACAACTTGGATCTTAAGTTACCAGGTAAAATCGCCATGAGTGTGAGTCCTTCGCAGCTTAAGTTTACAGAAGTTGGCCAGACAATTACATACCTAGCAGAGTTTATACCGGAAGATGGGGCAGGGAAGGATGCACCATTTTATCAGGGATATTTGAGATGGTTCTCTGATCAACATTCTGTTATTAGCCCCATATCTGTGATCTTCGACACCCAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

765

Amino Acids

81.06

Weight (kDa)

4.81

Isoelectric Point (pI)

34.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 59 - 131 9.2e-11 Peptidase inhibitor I9
Peptidase_S8 PF00082 154 - 579 4.8e-46 Subtilase family
PA PF02225 378 - 465 2.7e-12 PA domain
fn3_6 PF17766 660 - 759 1.7e-23 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1142, 1274
Acc36I ACCTGC 3 cut(s) 1142, 1274, 1373
Acc65I GGTACC 1 cut(s) 210
AccB1I GGYRCC 4 cut(s) 210, 605, 710, 754
AccB7I CCANNNNNTGG 1 cut(s) 289
AciI CCGC 3 cut(s) 1476, 1734, 1893
AclWI GGATC 2 cut(s) 1294, 2094
AcoI YGGCCR 3 cut(s) 291, 1428, 2152
AcsI RAATTY 3 cut(s) 135, 697, 945
AcuI CTGAAG 2 cut(s) 1974, 1992
AdeI CACNNNGTG 3 cut(s) 116, 289, 878
AfaI GTAC 4 cut(s) 102, 212, 779, 1633
AfiI CCNNNNNNNGG 2 cut(s) 289, 2192
AflII CTTAAG 2 cut(s) 2090, 2135
AflIII ACRYGT 4 cut(s) 675, 717, 1366, 1816
AjiI CACGTC 1 cut(s) 678
AjnI CCWGG 6 cut(s) 502, 618, 1509, 1843, 2010, 2098
AleI CACNNNNGTG 1 cut(s) 1656
Alw21I GWGCWC 5 cut(s) 643, 686, 1520, 1685, 1952
Alw26I GTCTC 3 cut(s) 113, 2025, 2031
AlwI GGATC 2 cut(s) 1294, 2094
AlwNI CAGNNNCTG 3 cut(s) 83, 746, 980
AoxI GGCC 8 cut(s) 158, 291, 661, 977, 1005, 1428, 1574, 2152
ApoI RAATTY 3 cut(s) 135, 697, 945
ArsI GACNNNNNNTTYG 2 cut(s) 1437, 1469
Asp718I GGTACC 1 cut(s) 210
AspLEI GCGC 1 cut(s) 1978
AspS9I GGNCC 6 cut(s) 977, 1006, 1194, 1502, 1547, 2060
AsuC2I CCSGG 1 cut(s) 1551
AsuHPI GGTGA 7 cut(s) 128, 1166, 1472, 1643, 1692, 1921, 2059
AvaII GGWCC 4 cut(s) 1194, 1502, 1547, 2060
BalI TGGCCA 3 cut(s) 293, 1430, 2154
BanI GGYRCC 4 cut(s) 210, 605, 710, 754
BanII GRGCYC 2 cut(s) 643, 1685
BbsI GAAGAC 1 cut(s) 1320
Bbv12I GWGCWC 5 cut(s) 643, 686, 1520, 1685, 1952
BccI CCATC 7 cut(s) 344, 842, 875, 1316, 1718, 2186, 2229
BceAI ACGGC 1 cut(s) 1623
BcgI CGANNNNNNTGC 2 cut(s) 1438, 1472
BciT130I CCWGG 6 cut(s) 504, 620, 1511, 1845, 2012, 2100
BciVI GTATCC 1 cut(s) 481
BclI TGATCA 3 cut(s) 187, 1462, 2245
BcnI CCSGG 1 cut(s) 1551
BcoDI GTCTC 3 cut(s) 113, 2025, 2031
BfaI CTAG 4 cut(s) 74, 821, 1235, 2171
BfmI CTRYAG 2 cut(s) 1327, 1380
BfrI CTTAAG 2 cut(s) 2090, 2135
BfuAI ACCTGC 3 cut(s) 1142, 1274, 1373
BfuI GTATCC 1 cut(s) 481
BlpI GCTNAGC 1 cut(s) 886
BmcAI AGTACT 1 cut(s) 102
Bme1390I CCNGG 7 cut(s) 504, 620, 1511, 1551, 1845, 2012, 2100
Bme18I GGWCC 4 cut(s) 1194, 1502, 1547, 2060
BmgBI CACGTC 1 cut(s) 678
BmgT120I GGNCC 6 cut(s) 977, 1006, 1194, 1502, 1547, 2060
BmrFI CCNGG 7 cut(s) 504, 620, 1511, 1551, 1845, 2012, 2100
BmrI ACTGGG 1 cut(s) 327
BmsI GCATC 5 cut(s) 15, 1333, 1401, 1569, 2197
BmuI ACTGGG 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 1320
BpmI CTGGAG 1 cut(s) 1770
Bpu1102I GCTNAGC 1 cut(s) 886
BpuEI CTTGAG 2 cut(s) 1661, 2013
BpuMI CCSGG 1 cut(s) 1551
BsaBI GATNNNNATC 1 cut(s) 29
BsaJI CCNNGG 6 cut(s) 619, 664, 1010, 1750, 1843, 1844
BsaWI WCCGGW 2 cut(s) 635, 2185
BsaXI ACNNNNNCTCC 8 cut(s) 625, 655, 1257, 1287, 1394, 1424, 1768, 1798
Bsc4I CCNNNNNNNGG 2 cut(s) 289, 2192
Bse1I ACTGG 7 cut(s) 108, 214, 322, 496, 647, 1700, 1810
Bse3DI GCAATG 3 cut(s) 654, 780, 1662
Bse8I GATNNNNATC 1 cut(s) 29
BseBI CCWGG 6 cut(s) 504, 620, 1511, 1845, 2012, 2100
BseDI CCNNGG 6 cut(s) 619, 664, 1010, 1750, 1843, 1844
BseGI GGATG 4 cut(s) 367, 508, 862, 2212
BseJI GATNNNNATC 1 cut(s) 29
BseLI CCNNNNNNNGG 2 cut(s) 289, 2192
BseMI GCAATG 3 cut(s) 654, 780, 1662
BseMII CTCAG 3 cut(s) 900, 1646, 2036
BseNI ACTGG 7 cut(s) 108, 214, 322, 496, 647, 1700, 1810
BseRI GAGGAG 1 cut(s) 531
BseYI CCCAGC 2 cut(s) 164, 2289
BsgI GTGCAG 2 cut(s) 483, 1830
BshFI GGCC 8 cut(s) 160, 293, 663, 979, 1007, 1430, 1576, 2154
BshNI GGYRCC 4 cut(s) 210, 605, 710, 754
BsiHKAI GWGCWC 5 cut(s) 643, 686, 1520, 1685, 1952
BsiSI CCGG 4 cut(s) 545, 636, 1551, 2186
BslFI GGGAC 3 cut(s) 450, 1180, 1533
BslI CCNNNNNNNGG 2 cut(s) 289, 2192
BsmAI GTCTC 3 cut(s) 113, 2025, 2031
BsmFI GGGAC 3 cut(s) 450, 1180, 1533
BsnI GGCC 8 cut(s) 160, 293, 663, 979, 1007, 1430, 1576, 2154
Bsp1286I GDGCHC 5 cut(s) 643, 686, 1520, 1685, 1952
Bsp143I GATC 8 cut(s) 30, 187, 1299, 1462, 1912, 2086, 2245, 2277
Bsp1720I GCTNAGC 1 cut(s) 886
Bsp19I CCATGG 2 cut(s) 664, 1010
BspACI CCGC 3 cut(s) 1476, 1734, 1893
BspANI GGCC 8 cut(s) 160, 293, 663, 979, 1007, 1430, 1576, 2154
BspCNI CTCAG 3 cut(s) 899, 1647, 2035
BspHI TCATGA 3 cut(s) 190, 255, 1305
BspMAI CTGCAG 1 cut(s) 1384
BspMI ACCTGC 3 cut(s) 1142, 1274, 1373
BspPI GGATC 2 cut(s) 1294, 2094
BspT107I GGYRCC 4 cut(s) 210, 605, 710, 754
BspTI CTTAAG 2 cut(s) 2090, 2135
BsrDI GCAATG 3 cut(s) 654, 780, 1662
BsrI ACTGG 7 cut(s) 108, 214, 322, 496, 647, 1700, 1810
BssECI CCNNGG 6 cut(s) 619, 664, 1010, 1750, 1843, 1844
BssMI GATC 8 cut(s) 30, 187, 1299, 1462, 1912, 2086, 2245, 2277
BssT1I CCWWGG 2 cut(s) 664, 1010
Bst2UI CCWGG 6 cut(s) 504, 620, 1511, 1845, 2012, 2100
Bst4CI ACNGT 5 cut(s) 1024, 1134, 1453, 1942, 2047
BstAFI CTTAAG 2 cut(s) 2090, 2135
BstAPI GCANNNNNTGC 4 cut(s) 585, 761, 920, 1289
BstC8I GCNNGC 4 cut(s) 131, 661, 1033, 1516
BstDEI CTNAG 4 cut(s) 886, 1564, 1655, 2022
BstDSI CCRYGG 2 cut(s) 664, 1010
BstEII GGTNACC 2 cut(s) 1154, 1698
BstF5I GGATG 4 cut(s) 367, 508, 862, 2212
BstHHI GCGC 1 cut(s) 1978
BstKTI GATC 8 cut(s) 33, 190, 1302, 1465, 1915, 2089, 2248, 2280
BstMAI GTCTC 3 cut(s) 113, 2025, 2031
BstMBI GATC 8 cut(s) 30, 187, 1299, 1462, 1912, 2086, 2245, 2277
BstNI CCWGG 6 cut(s) 504, 620, 1511, 1845, 2012, 2100
BstNSI RCATGY 4 cut(s) 61, 133, 1370, 1820
BstPI GGTNACC 2 cut(s) 1154, 1698
BstSCI CCNGG 7 cut(s) 502, 618, 1509, 1549, 1843, 2010, 2098
BstSFI CTRYAG 2 cut(s) 1327, 1380
BstV2I GAAGAC 1 cut(s) 1320
BstX2I RGATCY 1 cut(s) 2086
BstYI RGATCY 1 cut(s) 2086
BsuI GTATCC 1 cut(s) 481
BsuRI GGCC 8 cut(s) 160, 293, 663, 979, 1007, 1430, 1576, 2154
BtgI CCRYGG 2 cut(s) 664, 1010
BtgZI GCGATG 1 cut(s) 1200
BtrI CACGTC 1 cut(s) 678
BtsCI GGATG 4 cut(s) 367, 508, 862, 2212
BtsI GCAGTG 1 cut(s) 834
BtsIMutI CAGTG 6 cut(s) 834, 1317, 1449, 1590, 1803, 1947
BveI ACCTGC 3 cut(s) 1142, 1274, 1373
Cac8I GCNNGC 4 cut(s) 131, 661, 1033, 1516
CaiI CAGNNNCTG 3 cut(s) 83, 746, 980
CciI TCATGA 3 cut(s) 190, 255, 1305
CfoI GCGC 1 cut(s) 1978
Cfr13I GGNCC 6 cut(s) 977, 1006, 1194, 1502, 1547, 2060
CsiI ACCWGGT 2 cut(s) 502, 2098
Csp6I GTAC 4 cut(s) 101, 211, 778, 1632
CspCI CAANNNNNGTGG 2 cut(s) 283, 318
CviQI GTAC 4 cut(s) 101, 211, 778, 1632
DdeI CTNAG 4 cut(s) 886, 1564, 1655, 2022
DpnI GATC 8 cut(s) 32, 189, 1301, 1464, 1914, 2088, 2247, 2279
DpnII GATC 8 cut(s) 30, 187, 1299, 1462, 1912, 2086, 2245, 2277
DraIII CACNNNGTG 3 cut(s) 116, 289, 878
EaeI YGGCCR 3 cut(s) 291, 1428, 2152
Ecl136II GAGCTC 2 cut(s) 641, 1683
Eco130I CCWWGG 2 cut(s) 664, 1010
Eco24I GRGCYC 2 cut(s) 643, 1685
Eco47I GGWCC 4 cut(s) 1194, 1502, 1547, 2060
Eco53kI GAGCTC 2 cut(s) 641, 1683
Eco57I CTGAAG 2 cut(s) 1974, 1992
Eco91I GGTNACC 2 cut(s) 1154, 1698
EcoICRI GAGCTC 2 cut(s) 641, 1683
EcoO109I RGGNCCY 3 cut(s) 977, 1006, 1194
EcoO65I GGTNACC 2 cut(s) 1154, 1698
EcoRII CCWGG 6 cut(s) 502, 618, 1509, 1843, 2010, 2098
EcoT14I CCWWGG 2 cut(s) 664, 1010
EcoT22I ATGCAT 1 cut(s) 280
EcoT38I GRGCYC 2 cut(s) 643, 1685
ErhI CCWWGG 2 cut(s) 664, 1010
FaqI GGGAC 3 cut(s) 450, 1180, 1533
FauI CCCGC 1 cut(s) 1483
FbaI TGATCA 3 cut(s) 187, 1462, 2245
FokI GGATG 4 cut(s) 354, 495, 869, 2219
FriOI GRGCYC 2 cut(s) 643, 1685
FspBI CTAG 4 cut(s) 74, 821, 1235, 2171
GlaI GCGC 1 cut(s) 1977
GsaI CCCAGC 2 cut(s) 168, 2293
GsuI CTGGAG 1 cut(s) 1770
HaeIII GGCC 8 cut(s) 160, 293, 663, 979, 1007, 1430, 1576, 2154
HapII CCGG 4 cut(s) 545, 636, 1551, 2186
HhaI GCGC 1 cut(s) 1978
Hin6I GCGC 1 cut(s) 1976
HinP1I GCGC 1 cut(s) 1976
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 9 cut(s) 379, 468, 480, 1097, 1169, 1524, 1601, 1962, 2122
HpaII CCGG 4 cut(s) 545, 636, 1551, 2186
HphI GGTGA 7 cut(s) 128, 1166, 1472, 1643, 1692, 1921, 2059
Hpy166II GTNNAC 4 cut(s) 652, 1588, 1747, 2142
Hpy188I TCNGA 8 cut(s) 187, 793, 808, 939, 1754, 1917, 1954, 2245
Hpy188III TCNNGA 8 cut(s) 191, 256, 371, 1306, 1497, 1528, 1678, 2030
Hpy8I GTNNAC 4 cut(s) 652, 1588, 1747, 2142
Hpy99I CGWCG 2 cut(s) 527, 682
HpyAV CCTTC 7 cut(s) 50, 521, 934, 1205, 2001, 2136, 2197
HpyCH4III ACNGT 5 cut(s) 1024, 1134, 1453, 1942, 2047
HpyCH4IV ACGT 2 cut(s) 677, 717
HpyF3I CTNAG 4 cut(s) 886, 1564, 1655, 2022
HpySE526I ACGT 2 cut(s) 677, 717
HspAI GCGC 1 cut(s) 1976
KflI GGGWCCC 1 cut(s) 1194
KpnI GGTACC 1 cut(s) 214
Ksp22I TGATCA 3 cut(s) 187, 1462, 2245
Kzo9I GATC 8 cut(s) 30, 187, 1299, 1462, 1912, 2086, 2245, 2277
LmnI GCTCC 6 cut(s) 638, 646, 1028, 1040, 1477, 1789
LweI GCATC 5 cut(s) 15, 1333, 1401, 1569, 2197
MabI ACCWGGT 2 cut(s) 502, 2098
MaeI CTAG 4 cut(s) 74, 821, 1235, 2171
MaeII ACGT 2 cut(s) 677, 717
MaeIII GTNAC 9 cut(s) 69, 812, 1134, 1154, 1480, 1698, 1726, 2047, 2094
MalI GATC 8 cut(s) 32, 189, 1301, 1464, 1914, 2088, 2247, 2279
MboI GATC 8 cut(s) 30, 187, 1299, 1462, 1912, 2086, 2245, 2277
MboII GAAGA 8 cut(s) 206, 344, 355, 1263, 1280, 1325, 2201, 2272
MfeI CAATTG 3 cut(s) 386, 915, 1722
MflI RGATCY 1 cut(s) 2086
MhlI GDGCHC 5 cut(s) 643, 686, 1520, 1685, 1952
MlsI TGGCCA 3 cut(s) 293, 1430, 2154
MluNI TGGCCA 3 cut(s) 293, 1430, 2154
MlyI GAGTC 3 cut(s) 489, 1971, 2131
MmeI TCCRAC 2 cut(s) 1006, 2038
Mox20I TGGCCA 3 cut(s) 293, 1430, 2154
Mph1103I ATGCAT 1 cut(s) 280
MscI TGGCCA 3 cut(s) 293, 1430, 2154
MseI TTAA 4 cut(s) 1617, 1821, 2091, 2136
MslI CAYNNNNRTG 4 cut(s) 1164, 1656, 2117, 2273
Msp20I TGGCCA 3 cut(s) 293, 1430, 2154
MspA1I CMGCKG 4 cut(s) 689, 957, 1706, 1736
MspCI CTTAAG 2 cut(s) 2090, 2135
MspI CCGG 4 cut(s) 545, 636, 1551, 2186
MspR9I CCNGG 7 cut(s) 504, 620, 1511, 1551, 1845, 2012, 2100
MunI CAATTG 3 cut(s) 386, 915, 1722
MvaI CCWGG 6 cut(s) 504, 620, 1511, 1845, 2012, 2100
NciI CCSGG 1 cut(s) 1551
NcoI CCATGG 2 cut(s) 664, 1010
NdeII GATC 8 cut(s) 30, 187, 1299, 1462, 1912, 2086, 2245, 2277
NmuCI GTSAC 7 cut(s) 69, 812, 1154, 1480, 1698, 1726, 2047
NsiI ATGCAT 1 cut(s) 280
NspI RCATGY 4 cut(s) 61, 133, 1370, 1820
OliI CACNNNNGTG 1 cut(s) 1656
PaeI GCATGC 1 cut(s) 133
PagI TCATGA 3 cut(s) 190, 255, 1305
PaqCI CACCTGC 2 cut(s) 1142, 1274
PasI CCCWGGG 1 cut(s) 1844
PciI ACATGT 2 cut(s) 1366, 1816
PfeI GAWTC 6 cut(s) 379, 468, 1097, 1169, 1524, 1601
PflMI CCANNNNNTGG 1 cut(s) 289
PfoI TCCNGGA 1 cut(s) 2010
PleI GAGTC 3 cut(s) 488, 1970, 2130
PpsI GAGTC 3 cut(s) 488, 1970, 2130
PpuMI RGGWCCY 1 cut(s) 1194
PscI ACATGT 2 cut(s) 1366, 1816
Psp124BI GAGCTC 2 cut(s) 643, 1685
Psp5II RGGWCCY 1 cut(s) 1194
Psp6I CCWGG 6 cut(s) 502, 618, 1509, 1843, 2010, 2098
PspEI GGTNACC 2 cut(s) 1154, 1698
PspFI CCCAGC 2 cut(s) 164, 2289
PspGI CCWGG 6 cut(s) 502, 618, 1509, 1843, 2010, 2098
PspPI GGNCC 6 cut(s) 977, 1006, 1194, 1502, 1547, 2060
PspPPI RGGWCCY 1 cut(s) 1194
PstI CTGCAG 1 cut(s) 1384
PstNI CAGNNNCTG 3 cut(s) 83, 746, 980
PsuI RGATCY 1 cut(s) 2086
PvuII CAGCTG 3 cut(s) 689, 957, 1706
RsaI GTAC 4 cut(s) 102, 212, 779, 1633
RsaNI GTAC 4 cut(s) 101, 211, 778, 1632
RseI CAYNNNNRTG 4 cut(s) 1164, 1656, 2117, 2273
SacI GAGCTC 2 cut(s) 643, 1685
SaqAI TTAA 4 cut(s) 1617, 1821, 2091, 2136
Sau3AI GATC 8 cut(s) 30, 187, 1299, 1462, 1912, 2086, 2245, 2277
Sau96I GGNCC 6 cut(s) 977, 1006, 1194, 1502, 1547, 2060
ScaI AGTACT 1 cut(s) 102
SchI GAGTC 3 cut(s) 489, 1971, 2131
ScrFI CCNGG 7 cut(s) 504, 620, 1511, 1551, 1845, 2012, 2100
SduI GDGCHC 5 cut(s) 643, 686, 1520, 1685, 1952
SexAI ACCWGGT 2 cut(s) 502, 2098
SfaNI GCATC 5 cut(s) 15, 1333, 1401, 1569, 2197
SfcI CTRYAG 2 cut(s) 1327, 1380
SinI GGWCC 4 cut(s) 1194, 1502, 1547, 2060
SmiMI CAYNNNNRTG 4 cut(s) 1164, 1656, 2117, 2273
SmlI CTYRAG 4 cut(s) 1676, 2028, 2090, 2135
SmoI CTYRAG 4 cut(s) 1676, 2028, 2090, 2135
SphI GCATGC 1 cut(s) 133
SsiI CCGC 3 cut(s) 1476, 1734, 1893
SspMI CTAG 4 cut(s) 74, 821, 1235, 2171
SstI GAGCTC 2 cut(s) 643, 1685
StyD4I CCNGG 7 cut(s) 502, 618, 1509, 1549, 1843, 2010, 2098
StyI CCWWGG 2 cut(s) 664, 1010
TaaI ACNGT 5 cut(s) 1024, 1134, 1453, 1942, 2047
TaiI ACGT 2 cut(s) 680, 720
TaqI TCGA 3 cut(s) 87, 680, 2283
TatI WGTACW 1 cut(s) 100
TfiI GAWTC 6 cut(s) 379, 468, 1097, 1169, 1524, 1601
Tru1I TTAA 4 cut(s) 1617, 1821, 2091, 2136
Tru9I TTAA 4 cut(s) 1617, 1821, 2091, 2136
TscAI CASTG 6 cut(s) 841, 1324, 1456, 1590, 1810, 1947
TseFI GTSAC 7 cut(s) 69, 812, 1154, 1480, 1698, 1726, 2047
Tsp45I GTSAC 7 cut(s) 69, 812, 1154, 1480, 1698, 1726, 2047
TspDTI ATGAA 7 cut(s) 207, 272, 1161, 1322, 1482, 1788, 1983
TspGWI ACGGA 2 cut(s) 391, 1886
TspRI CASTG 6 cut(s) 841, 1324, 1456, 1590, 1810, 1947
Van91I CCANNNNNTGG 1 cut(s) 289
Vha464I CTTAAG 2 cut(s) 2090, 2135
VpaK11BI GGWCC 4 cut(s) 1194, 1502, 1547, 2060
XapI RAATTY 3 cut(s) 135, 697, 945
XceI RCATGY 4 cut(s) 61, 133, 1370, 1820
XspI CTAG 4 cut(s) 74, 821, 1235, 2171
ZrmI AGTACT 1 cut(s) 102
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.