Rw7G016080

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000302
Physical Location & Seq
Reverse (-)
16447827 .. 16452527
4701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G016080.1

Sequence Viewer

Length: 2571 bp
ATGGAGAACAAAAAGTGTGTAGTGATGCAGATCATATACCTTCTTGGCTTTAGTTGCATGTTTTACTTGTCACTAGCAGTTGCTGTCGAAAAAGTGGCAGTACTGGAACCACAAGGTGAGACAGATAGCATGCAAATTTATATAGTTTTGGTTGAAAGGCCACCCAGCAATTTCAATGCACAATCTGATCATGAAGATTTAGAGAGTTGGTACCAGTCATTTCTGCCCCAAACTATTGCAAACTCAAACCAGCTCATGAAGCCACGAATGGTTTATGCATACCACAATGTGGCCACAGGATTTGCAGCAAAGCTGACTGGGGAGGAAGTTGAAGAAATGGAGAAGAAAGATGGGTTTATCTACGCTCATCCAGAACGGAATCTGCAATTGCACACAACTCATAGTCCTAACTTCTTGGGGCTGCAACAAGGATTGGGACTTTGGAAAGGGTCTAATTATGGTGCAGGAATCATTATTGGAGTCTTGGATACTGGAATAACACCAGGTCATCCCTCATTCAGCGACGAAGGAGTGCCACCTCCTCCGGCTAAATGGAAAGGCAAGTGTGATTTCAATGGCACATTCTGCAACAACAAGCTTATTGGTGCCAGAAGTTTCCAGGGTGGGAACACTACCGGAGCTCCACCAGTTGACATTGCAGGCCATGGAACCCACACGTCGAGCACAGCTGGTGGAAATTTTGTGAAAGGTGCCAACGTGTTTGGAATGGCAAATGGAACAGCCTCTGGCATGGCACCTTATGCTCACTTGGCAATGTACCAAGTTTGTTCTGAACAGGGTTGTTCTGAAAGTGACATTCTAGCTGCTATTGACACTGCTATTGATGATGGCGTGGATGTGCTATCCATCTCACTTGGTGGTGGCTCAGCTACTTTTTATTGGGATTTTATTGCAATTGGTGCATTTGCAGCAATTCAGAAGGGAATTTTTGTCAGCTGTTCGGCTGGAAATGCAGGGCCTGACTACTCTACTACATCAAATGAGGCCCCATGGATTTTGACAGTTGGAGCAAGCTCCATTGACAGAAACATAAGAGCAATAGCACAGCTTGGAAATGGGCAAGAATATGACGGGGAATCAGTATTCCAGCCTAAAGATTTTAGTTCAAAACAGTTACCTCTTGTTTATGCAGGTGACCATAGCCATGATTCATCACCTCTTTGCGATGTAGGGTCCCTTACAAATGTTGAAGGGAAAGTAGTGGTGTGTGAGCTAGGTGGCGGAATTGGAAGAATTGCAAAAGGGGTAGAAGTGAAAAGAGCTGGTGGTGTTGCCATGATTCTTGTCAACCCAGACTTTGCTGGCTATACCACCATAGCAGACGTTCACGTGCTTCCGGCAACACATGTAAGTTACTCTGCGGGGGTGAGTATCAAAACCTATATAAACTCAACCTCAACTCCTACAGCAACAATCTTGTTCAAAGGCACTGTCATTGGTGATCAGCTTGCTCCGAAGGTCTCTTTCTTTTCATCAAGAGGACCAAGCCTCGCAAGTCCTGGCATTTTGAAACCTGACATAATTGGTCCCGGTGTGAGCATCCTAGCTGCATGGCCTTTTTCGGTGGACAATGCCACAGAATCTAAAGCCACATTTAACATTATTTCCGGTACTTCAATGTCATGCCCTCACCTGAGTGGCATTGTAGCCTTACTCAAGAGCTCACACCCTGACTGGTCACCAGCTGCCATTAAGTCTGCCATCATGACAACTGCTGAAGTAAACAACCTCGCAGGCTCTGCCATAATGGATGAAACACTATTTGCAGCAGACCTCTTTGCCATTGGTGCAGGTCATGTTAACCCTTCAAAAGCAAATGACCCTGGGCTCATCTACGACACGCAACCAGATGACTACATTCCTTACATGTGTGGTTTGAACTACACAGACCAACAGATAGCAATAGTCACCCGGAAATTAGTGAAATGCTCTGAAGTAGGAGCCATACCCGAAGCACAGCTAAATTATCCCACATTTTCTCTCTTTATAGATCCTGCAGGGAAGCCTCAGCATTACACAAGAACAGTGACGAATGTTGGCCCCGCTAAATCAACTTATGAAGTAGCCGTTGTAAGTCCACATAACATAGACATCAGTGTGCAACCTGAGGAGCTCACATTCACAGAGGTTAACCAGACAATGACGTATCATGTGGTGTTTACCGCACAAAAAGGTGCTGCGAAGAATGATCACAACAGAATGAGAACCGGTGGACATGGATATGTAACATGTGGACAGTCTCAAGAGAATAATCTTCAAATAATTACTGATACTGTTCGCTACTACCTGCAAAGACAGAAGCAAAAGGCAATGTTCAAGCACTGGCTCAACAGCTCACACCCTAACTGGTCGCCAGCTGCCATTAAGTCTGCAATCAAGACAACCGCCGATGTACTAAACCTCGGAGGCAAGCCCATTGTTGATGAAAGACATGCTCCAGCAGACATCTTTGCCACTGGTGCAGGCCATGTCAAACCTTCAAAAGCAAATGACCCTGGGCTCATCTACGACCTAACCCTGAAGATTACATCCCCTACTTGTGCGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

856

Amino Acids

91.17

Weight (kDa)

5.75

Isoelectric Point (pI)

33.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 59 - 131 1.1e-10 Peptidase inhibitor I9
Peptidase_S8 PF00082 154 - 582 1.4e-45 Subtilase family
PA PF02225 378 - 464 1.4e-10 PA domain
fn3_6 PF17766 660 - 736 7.3e-20 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1142
Acc36I ACCTGC 3 cut(s) 1142, 1802, 2316
Acc65I GGTACC 1 cut(s) 210
AccB1I GGYRCC 4 cut(s) 210, 605, 710, 754
AccB7I CCANNNNNTGG 1 cut(s) 289
AciI CCGC 6 cut(s) 1242, 1382, 2064, 2184, 2406, 2564
AclWI GGATC 1 cut(s) 2006
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 3 cut(s) 135, 697, 945
AcuI CTGAAG 3 cut(s) 1758, 1974, 2561
AcvI CACGTG 1 cut(s) 1351
AdeI CACNNNGTG 3 cut(s) 116, 289, 878
AfaI GTAC 5 cut(s) 102, 212, 779, 1633, 2415
AfiI CCNNNNNNNGG 1 cut(s) 289
AflIII ACRYGT 5 cut(s) 675, 717, 1366, 1887, 2249
AgeI ACCGGT 1 cut(s) 2228
AjiI CACGTC 1 cut(s) 678
AjnI CCWGG 5 cut(s) 502, 618, 1519, 1843, 2515
AleI CACNNNNGTG 1 cut(s) 1656
Alw21I GWGCWC 4 cut(s) 643, 686, 1685, 2136
Alw26I GTCTC 3 cut(s) 113, 1486, 2265
AlwI GGATC 1 cut(s) 2006
AlwNI CAGNNNCTG 4 cut(s) 83, 746, 980, 1760
AoxI GGCC 8 cut(s) 158, 291, 661, 977, 1005, 1574, 2059, 2485
ApeKI GCWGC 9 cut(s) 305, 421, 824, 929, 1568, 1706, 1787, 2198, 2378
ApoI RAATTY 3 cut(s) 135, 697, 945
ArsI GACNNNNNNTTYG 2 cut(s) 1437, 1469
AsiGI ACCGGT 1 cut(s) 2228
Asp718I GGTACC 1 cut(s) 210
AspS9I GGNCC 6 cut(s) 977, 1006, 1194, 1502, 1547, 2060
AsuC2I CCSGG 2 cut(s) 1551, 1933
AsuHPI GGTGA 8 cut(s) 128, 1166, 1167, 1399, 1472, 1643, 1692, 1921
AvaII GGWCC 3 cut(s) 1194, 1502, 1547
AxyI CCTNAGG 1 cut(s) 2127
BalI TGGCCA 1 cut(s) 293
BanI GGYRCC 4 cut(s) 210, 605, 710, 754
BanII GRGCYC 5 cut(s) 643, 1685, 1851, 2136, 2523
BbrPI CACGTG 1 cut(s) 1351
Bbv12I GWGCWC 4 cut(s) 643, 686, 1685, 2136
BbvCI CCTCAGC 1 cut(s) 2028
BbvI GCAGC 9 cut(s) 317, 408, 811, 941, 1555, 1693, 1799, 2185, 2365
BccI CCATC 4 cut(s) 344, 842, 875, 1730
BceAI ACGGC 1 cut(s) 2072
BciT130I CCWGG 5 cut(s) 504, 620, 1521, 1845, 2517
BciVI GTATCC 1 cut(s) 481
BclI TGATCA 3 cut(s) 187, 1462, 2209
BcnI CCSGG 2 cut(s) 1551, 1933
BcoDI GTCTC 3 cut(s) 113, 1486, 2265
BfaI CTAG 4 cut(s) 74, 821, 1235, 1565
BfmI CTRYAG 2 cut(s) 1425, 2016
BfuAI ACCTGC 3 cut(s) 1142, 1802, 2316
BfuI GTATCC 1 cut(s) 481
BisI GCNGC 9 cut(s) 306, 422, 825, 930, 1569, 1707, 1788, 2199, 2379
BlpI GCTNAGC 1 cut(s) 886
BlsI GCNGC 9 cut(s) 307, 423, 826, 931, 1570, 1708, 1789, 2200, 2380
BmcAI AGTACT 1 cut(s) 102
Bme1390I CCNGG 7 cut(s) 504, 620, 1521, 1551, 1845, 1933, 2517
Bme18I GGWCC 3 cut(s) 1194, 1502, 1547
BmgBI CACGTC 1 cut(s) 678
BmgT120I GGNCC 6 cut(s) 977, 1006, 1194, 1502, 1547, 2060
BmrFI CCNGG 7 cut(s) 504, 620, 1521, 1551, 1845, 1933, 2517
BmrI ACTGGG 1 cut(s) 327
BmsI GCATC 2 cut(s) 15, 1569
BmuI ACTGGG 1 cut(s) 327
BpmI CTGGAG 1 cut(s) 2442
Bpu10I CCTNAGC 1 cut(s) 2028
Bpu1102I GCTNAGC 1 cut(s) 886
BpuEI CTTGAG 2 cut(s) 1661, 2247
BpuMI CCSGG 2 cut(s) 1551, 1933
BsaAI YACGTR 1 cut(s) 1351
BsaBI GATNNNNATC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 1486
BsaJI CCNNGG 8 cut(s) 619, 664, 1010, 1843, 1844, 2422, 2515, 2516
BsaWI WCCGGW 3 cut(s) 635, 1628, 2228
BsaXI ACNNNNNCTCC 4 cut(s) 625, 655, 1405, 1435
Bsc4I CCNNNNNNNGG 1 cut(s) 289
Bse118I RCCGGY 1 cut(s) 2228
Bse1I ACTGG 9 cut(s) 108, 214, 322, 496, 647, 1700, 2348, 2372, 2482
Bse21I CCTNAGG 1 cut(s) 2127
Bse3DI GCAATG 3 cut(s) 654, 780, 2337
Bse8I GATNNNNATC 1 cut(s) 29
BseBI CCWGG 5 cut(s) 504, 620, 1521, 1845, 2517
BseDI CCNNGG 8 cut(s) 619, 664, 1010, 1843, 1844, 2422, 2515, 2516
BseGI GGATG 6 cut(s) 367, 508, 862, 1560, 1777, 2549
BseJI GATNNNNATC 1 cut(s) 29
BseLI CCNNNNNNNGG 1 cut(s) 289
BseMI GCAATG 3 cut(s) 654, 780, 2337
BseMII CTCAG 4 cut(s) 900, 1646, 2042, 2118
BseNI ACTGG 9 cut(s) 108, 214, 322, 496, 647, 1700, 2348, 2372, 2482
BseRI GAGGAG 2 cut(s) 531, 2144
BseXI GCAGC 9 cut(s) 317, 408, 811, 941, 1555, 1693, 1799, 2185, 2365
BseYI CCCAGC 1 cut(s) 164
BsgI GTGCAG 3 cut(s) 483, 1830, 2502
BshFI GGCC 8 cut(s) 160, 293, 663, 979, 1007, 1576, 2061, 2487
BshNI GGYRCC 4 cut(s) 210, 605, 710, 754
BshTI ACCGGT 1 cut(s) 2228
BsiHKAI GWGCWC 4 cut(s) 643, 686, 1685, 2136
BsiSI CCGG 7 cut(s) 545, 636, 1358, 1551, 1629, 1933, 2229
BslFI GGGAC 3 cut(s) 450, 1180, 1533
BslI CCNNNNNNNGG 1 cut(s) 289
BsmAI GTCTC 3 cut(s) 113, 1486, 2265
BsmFI GGGAC 3 cut(s) 450, 1180, 1533
BsnI GGCC 8 cut(s) 160, 293, 663, 979, 1007, 1576, 2061, 2487
Bso31I GGTCTC 1 cut(s) 1486
Bsp1286I GDGCHC 6 cut(s) 643, 686, 1685, 1851, 2136, 2523
Bsp143I GATC 5 cut(s) 30, 187, 1462, 2011, 2209
Bsp1720I GCTNAGC 1 cut(s) 886
Bsp19I CCATGG 2 cut(s) 664, 1010
BspACI CCGC 6 cut(s) 1242, 1382, 2064, 2184, 2406, 2564
BspANI GGCC 8 cut(s) 160, 293, 663, 979, 1007, 1576, 2061, 2487
BspCNI CTCAG 4 cut(s) 899, 1647, 2041, 2119
BspHI TCATGA 3 cut(s) 190, 255, 1725
BspMAI CTGCAG 1 cut(s) 2020
BspMI ACCTGC 3 cut(s) 1142, 1802, 2316
BspPI GGATC 1 cut(s) 2006
BspT107I GGYRCC 4 cut(s) 210, 605, 710, 754
BspTNI GGTCTC 1 cut(s) 1486
BsrDI GCAATG 3 cut(s) 654, 780, 2337
BsrFI RCCGGY 1 cut(s) 2228
BsrI ACTGG 9 cut(s) 108, 214, 322, 496, 647, 1700, 2348, 2372, 2482
BssAI RCCGGY 1 cut(s) 2228
BssECI CCNNGG 8 cut(s) 619, 664, 1010, 1843, 1844, 2422, 2515, 2516
BssMI GATC 5 cut(s) 30, 187, 1462, 2011, 2209
BssT1I CCWWGG 2 cut(s) 664, 1010
Bst2UI CCWGG 5 cut(s) 504, 620, 1521, 1845, 2517
Bst4CI ACNGT 6 cut(s) 1024, 1134, 1453, 2047, 2259, 2296
BstAPI GCANNNNNTGC 4 cut(s) 585, 761, 920, 1760
BstBAI YACGTR 1 cut(s) 1351
BstC8I GCNNGC 9 cut(s) 131, 661, 1033, 1324, 1470, 1756, 2376, 2432, 2485
BstDEI CTNAG 4 cut(s) 886, 1655, 2028, 2127
BstDSI CCRYGG 2 cut(s) 664, 1010
BstEII GGTNACC 2 cut(s) 1154, 1698
BstF5I GGATG 6 cut(s) 367, 508, 862, 1560, 1777, 2549
BstKTI GATC 5 cut(s) 33, 190, 1465, 2014, 2212
BstMAI GTCTC 3 cut(s) 113, 1486, 2265
BstMBI GATC 5 cut(s) 30, 187, 1462, 2011, 2209
BstNI CCWGG 5 cut(s) 504, 620, 1521, 1845, 2517
BstNSI RCATGY 6 cut(s) 61, 133, 1370, 1891, 2253, 2456
BstPI GGTNACC 2 cut(s) 1154, 1698
BstSCI CCNGG 7 cut(s) 502, 618, 1519, 1549, 1843, 1931, 2515
BstSFI CTRYAG 2 cut(s) 1425, 2016
BstV1I GCAGC 9 cut(s) 317, 408, 811, 941, 1555, 1693, 1799, 2185, 2365
BstX2I RGATCY 1 cut(s) 2011
BstYI RGATCY 1 cut(s) 2011
Bsu36I CCTNAGG 1 cut(s) 2127
BsuI GTATCC 1 cut(s) 481
BsuRI GGCC 8 cut(s) 160, 293, 663, 979, 1007, 1576, 2061, 2487
BtgI CCRYGG 2 cut(s) 664, 1010
BtgZI GCGATG 1 cut(s) 1200
BtrI CACGTC 1 cut(s) 678
BtsCI GGATG 6 cut(s) 367, 508, 862, 1560, 1777, 2549
BtsI GCAGTG 1 cut(s) 834
BtsIMutI CAGTG 6 cut(s) 834, 1449, 2052, 2122, 2341, 2475
BveI ACCTGC 3 cut(s) 1142, 1802, 2316
Cac8I GCNNGC 9 cut(s) 131, 661, 1033, 1324, 1470, 1756, 2376, 2432, 2485
CaiI CAGNNNCTG 4 cut(s) 83, 746, 980, 1760
CciI TCATGA 3 cut(s) 190, 255, 1725
Cfr10I RCCGGY 1 cut(s) 2228
Cfr13I GGNCC 6 cut(s) 977, 1006, 1194, 1502, 1547, 2060
CsiI ACCWGGT 1 cut(s) 502
Csp6I GTAC 5 cut(s) 101, 211, 778, 1632, 2414
CspAI ACCGGT 1 cut(s) 2228
CspCI CAANNNNNGTGG 2 cut(s) 283, 318
CviQI GTAC 5 cut(s) 101, 211, 778, 1632, 2414
DdeI CTNAG 4 cut(s) 886, 1655, 2028, 2127
DpnI GATC 5 cut(s) 32, 189, 1464, 2013, 2211
DpnII GATC 5 cut(s) 30, 187, 1462, 2011, 2209
DraIII CACNNNGTG 3 cut(s) 116, 289, 878
EaeI YGGCCR 1 cut(s) 291
EciI GGCGGA 1 cut(s) 1257
Ecl136II GAGCTC 3 cut(s) 641, 1683, 2134
Eco130I CCWWGG 2 cut(s) 664, 1010
Eco24I GRGCYC 5 cut(s) 643, 1685, 1851, 2136, 2523
Eco31I GGTCTC 1 cut(s) 1486
Eco47I GGWCC 3 cut(s) 1194, 1502, 1547
Eco53kI GAGCTC 3 cut(s) 641, 1683, 2134
Eco57I CTGAAG 3 cut(s) 1758, 1974, 2561
Eco72I CACGTG 1 cut(s) 1351
Eco81I CCTNAGG 1 cut(s) 2127
Eco91I GGTNACC 2 cut(s) 1154, 1698
EcoICRI GAGCTC 3 cut(s) 641, 1683, 2134
EcoO109I RGGNCCY 3 cut(s) 977, 1006, 1194
EcoO65I GGTNACC 2 cut(s) 1154, 1698
EcoRII CCWGG 5 cut(s) 502, 618, 1519, 1843, 2515
EcoT14I CCWWGG 2 cut(s) 664, 1010
EcoT22I ATGCAT 1 cut(s) 280
EcoT38I GRGCYC 5 cut(s) 643, 1685, 1851, 2136, 2523
ErhI CCWWGG 2 cut(s) 664, 1010
FaqI GGGAC 3 cut(s) 450, 1180, 1533
FauI CCCGC 2 cut(s) 1375, 2071
FbaI TGATCA 3 cut(s) 187, 1462, 2209
Fnu4HI GCNGC 9 cut(s) 306, 422, 825, 930, 1569, 1707, 1788, 2199, 2379
FokI GGATG 6 cut(s) 354, 495, 869, 1547, 1784, 2536
FriOI GRGCYC 5 cut(s) 643, 1685, 1851, 2136, 2523
Fsp4HI GCNGC 9 cut(s) 306, 422, 825, 930, 1569, 1707, 1788, 2199, 2379
FspBI CTAG 4 cut(s) 74, 821, 1235, 1565
GluI GCNGC 9 cut(s) 306, 422, 825, 930, 1569, 1707, 1788, 2199, 2379
GsaI CCCAGC 1 cut(s) 168
GsuI CTGGAG 1 cut(s) 2442
HaeIII GGCC 8 cut(s) 160, 293, 663, 979, 1007, 1576, 2061, 2487
HapII CCGG 7 cut(s) 545, 636, 1358, 1551, 1629, 1933, 2229
HincII GTYRAC 4 cut(s) 652, 1309, 1822, 2152
HindII GTYRAC 4 cut(s) 652, 1309, 1822, 2152
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 7 cut(s) 379, 468, 480, 1097, 1169, 1300, 1601
HpaI GTTAAC 2 cut(s) 1822, 2152
HpaII CCGG 7 cut(s) 545, 636, 1358, 1551, 1629, 1933, 2229
HphI GGTGA 8 cut(s) 128, 1166, 1167, 1399, 1472, 1643, 1692, 1921
Hpy188I TCNGA 7 cut(s) 187, 793, 808, 939, 1476, 1954, 2426
Hpy188III TCNNGA 8 cut(s) 191, 256, 371, 1497, 1678, 1726, 2264, 2398
Hpy99I CGWCG 2 cut(s) 527, 682
HpyAV CCTTC 7 cut(s) 50, 521, 934, 1205, 1471, 1836, 2508
HpyCH4III ACNGT 6 cut(s) 1024, 1134, 1453, 2047, 2259, 2296
HpyCH4IV ACGT 5 cut(s) 677, 717, 1344, 1350, 2165
HpyF3I CTNAG 4 cut(s) 886, 1655, 2028, 2127
HpySE526I ACGT 5 cut(s) 677, 717, 1344, 1350, 2165
KflI GGGWCCC 1 cut(s) 1194
KpnI GGTACC 1 cut(s) 214
Ksp22I TGATCA 3 cut(s) 187, 1462, 2209
KspAI GTTAAC 2 cut(s) 1822, 2152
Kzo9I GATC 5 cut(s) 30, 187, 1462, 2011, 2209
LmnI GCTCC 8 cut(s) 638, 646, 1028, 1040, 1477, 1961, 2131, 2461
Lsp1109I GCAGC 9 cut(s) 317, 408, 811, 941, 1555, 1693, 1799, 2185, 2365
LweI GCATC 2 cut(s) 15, 1569
MabI ACCWGGT 1 cut(s) 502
MaeI CTAG 4 cut(s) 74, 821, 1235, 1565
MaeII ACGT 5 cut(s) 677, 717, 1344, 1350, 2165
MaeIII GTNAC 9 cut(s) 69, 812, 1134, 1154, 1373, 1698, 1927, 2047, 2245
MalI GATC 5 cut(s) 32, 189, 1464, 2013, 2211
MboI GATC 5 cut(s) 30, 187, 1462, 2011, 2209
MboII GAAGA 7 cut(s) 206, 344, 355, 1263, 2215, 2267, 2554
MfeI CAATTG 2 cut(s) 386, 915
MflI RGATCY 1 cut(s) 2011
MhlI GDGCHC 6 cut(s) 643, 686, 1685, 1851, 2136, 2523
MlsI TGGCCA 1 cut(s) 293
MluNI TGGCCA 1 cut(s) 293
MlyI GAGTC 1 cut(s) 489
MmeI TCCRAC 1 cut(s) 1006
Mox20I TGGCCA 1 cut(s) 293
Mph1103I ATGCAT 1 cut(s) 280
MscI TGGCCA 1 cut(s) 293
MseI TTAA 5 cut(s) 1617, 1713, 1821, 2151, 2385
MslI CAYNNNNRTG 4 cut(s) 1164, 1656, 2117, 2241
Msp20I TGGCCA 1 cut(s) 293
MspA1I CMGCKG 4 cut(s) 689, 957, 1706, 2378
MspI CCGG 7 cut(s) 545, 636, 1358, 1551, 1629, 1933, 2229
MspR9I CCNGG 7 cut(s) 504, 620, 1521, 1551, 1845, 1933, 2517
MunI CAATTG 2 cut(s) 386, 915
MvaI CCWGG 5 cut(s) 504, 620, 1521, 1845, 2517
NciI CCSGG 2 cut(s) 1551, 1933
NcoI CCATGG 2 cut(s) 664, 1010
NdeII GATC 5 cut(s) 30, 187, 1462, 2011, 2209
NmuCI GTSAC 6 cut(s) 69, 812, 1154, 1698, 1927, 2047
NsiI ATGCAT 1 cut(s) 280
NspI RCATGY 6 cut(s) 61, 133, 1370, 1891, 2253, 2456
OliI CACNNNNGTG 1 cut(s) 1656
PaeI GCATGC 1 cut(s) 133
PagI TCATGA 3 cut(s) 190, 255, 1725
PaqCI CACCTGC 1 cut(s) 1142
PasI CCCWGGG 2 cut(s) 1844, 2516
PciI ACATGT 3 cut(s) 1366, 1887, 2249
PfeI GAWTC 6 cut(s) 379, 468, 1097, 1169, 1300, 1601
PflMI CCANNNNNTGG 1 cut(s) 289
PinAI ACCGGT 1 cut(s) 2228
PkrI GCNGC 9 cut(s) 307, 423, 826, 931, 1570, 1708, 1789, 2200, 2380
PleI GAGTC 1 cut(s) 488
PmaCI CACGTG 1 cut(s) 1351
PmlI CACGTG 1 cut(s) 1351
PpsI GAGTC 1 cut(s) 488
Ppu21I YACGTR 1 cut(s) 1351
PpuMI RGGWCCY 1 cut(s) 1194
PscI ACATGT 3 cut(s) 1366, 1887, 2249
Psp124BI GAGCTC 3 cut(s) 643, 1685, 2136
Psp5II RGGWCCY 1 cut(s) 1194
Psp6I CCWGG 5 cut(s) 502, 618, 1519, 1843, 2515
PspCI CACGTG 1 cut(s) 1351
PspEI GGTNACC 2 cut(s) 1154, 1698
PspFI CCCAGC 1 cut(s) 164
PspGI CCWGG 5 cut(s) 502, 618, 1519, 1843, 2515
PspPI GGNCC 6 cut(s) 977, 1006, 1194, 1502, 1547, 2060
PspPPI RGGWCCY 1 cut(s) 1194
PstI CTGCAG 1 cut(s) 2020
PstNI CAGNNNCTG 4 cut(s) 83, 746, 980, 1760
PsuI RGATCY 1 cut(s) 2011
PvuII CAGCTG 4 cut(s) 689, 957, 1706, 2378
RsaI GTAC 5 cut(s) 102, 212, 779, 1633, 2415
RsaNI GTAC 5 cut(s) 101, 211, 778, 1632, 2414
RseI CAYNNNNRTG 4 cut(s) 1164, 1656, 2117, 2241
SacI GAGCTC 3 cut(s) 643, 1685, 2136
SaqAI TTAA 5 cut(s) 1617, 1713, 1821, 2151, 2385
SatI GCNGC 9 cut(s) 306, 422, 825, 930, 1569, 1707, 1788, 2199, 2379
Sau3AI GATC 5 cut(s) 30, 187, 1462, 2011, 2209
Sau96I GGNCC 6 cut(s) 977, 1006, 1194, 1502, 1547, 2060
SbfI CCTGCAGG 1 cut(s) 2020
ScaI AGTACT 1 cut(s) 102
SchI GAGTC 1 cut(s) 489
ScrFI CCNGG 7 cut(s) 504, 620, 1521, 1551, 1845, 1933, 2517
SdaI CCTGCAGG 1 cut(s) 2020
SduI GDGCHC 6 cut(s) 643, 686, 1685, 1851, 2136, 2523
SexAI ACCWGGT 1 cut(s) 502
SfaNI GCATC 2 cut(s) 15, 1569
SfcI CTRYAG 2 cut(s) 1425, 2016
SinI GGWCC 3 cut(s) 1194, 1502, 1547
SmiMI CAYNNNNRTG 4 cut(s) 1164, 1656, 2117, 2241
SmlI CTYRAG 2 cut(s) 1676, 2262
SmoI CTYRAG 2 cut(s) 1676, 2262
SphI GCATGC 1 cut(s) 133
Sse8387I CCTGCAGG 1 cut(s) 2020
SsiI CCGC 6 cut(s) 1242, 1382, 2064, 2184, 2406, 2564
SspMI CTAG 4 cut(s) 74, 821, 1235, 1565
SstI GAGCTC 3 cut(s) 643, 1685, 2136
StyD4I CCNGG 7 cut(s) 502, 618, 1519, 1549, 1843, 1931, 2515
StyI CCWWGG 2 cut(s) 664, 1010
TaaI ACNGT 6 cut(s) 1024, 1134, 1453, 2047, 2259, 2296
TaiI ACGT 5 cut(s) 680, 720, 1347, 1353, 2168
TaqI TCGA 2 cut(s) 87, 680
TatI WGTACW 2 cut(s) 100, 2413
TfiI GAWTC 6 cut(s) 379, 468, 1097, 1169, 1300, 1601
Tru1I TTAA 5 cut(s) 1617, 1713, 1821, 2151, 2385
Tru9I TTAA 5 cut(s) 1617, 1713, 1821, 2151, 2385
TscAI CASTG 6 cut(s) 841, 1456, 2052, 2122, 2348, 2482
TseFI GTSAC 6 cut(s) 69, 812, 1154, 1698, 1927, 2047
TseI GCWGC 9 cut(s) 305, 421, 824, 929, 1568, 1706, 1787, 2198, 2378
Tsp45I GTSAC 6 cut(s) 69, 812, 1154, 1698, 1927, 2047
TspDTI ATGAA 7 cut(s) 207, 272, 1161, 1482, 1788, 2094, 2460
TspGWI ACGGA 1 cut(s) 391
TspRI CASTG 6 cut(s) 841, 1456, 2052, 2122, 2348, 2482
Van91I CCANNNNNTGG 1 cut(s) 289
VpaK11BI GGWCC 3 cut(s) 1194, 1502, 1547
XapI RAATTY 3 cut(s) 135, 697, 945
XceI RCATGY 6 cut(s) 61, 133, 1370, 1891, 2253, 2456
XspI CTAG 4 cut(s) 74, 821, 1235, 1565
ZrmI AGTACT 1 cut(s) 102
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.