pycom04g02960

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000302
Physical Location & Seq
Reverse (-)
2711013 .. 2712539
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g02960.1

Sequence Viewer

Length: 1527 bp
ATGGAGAACAAGAAAGGTGCAGCAATGCATGTCATATATCTTCTTGGTTTTACTCTGATCATGTTTTACTTATCAAAGGCAGTTGCACAAGAGGAGCAAAATACCCTCCAAACTTACATTGTTTTTCTCGAAAAGCCGGCCTCCTCAAAATTTTCTGAAGAATCAGATTTAGACAGTTGGTACCAGTCATTTTTGCCTGATACTACAACGAACTTAAACCAGCAGAGGATCGTTCATGCATACCGCAGTGCCGTCACTGGATTTGCAGCTAAACTGACACCAGAGGAAGTGAAAGCAATGGAAAACAAAGAAGGGTTTTTGTCGGCTCATCTTGAGAAAACGGTTCCTTTGCTAACTACTCATAGTCCTGACTTCTTGGGGTTACACCAAGGATACGGACTATGGAAACAAGCAAATTACGGTGATGGTGTAATAATAGGACTCTTGGATACAGGCATTGGACCCGATCATCCTTCATTCAGTGACGAAGGAGTATCCCCTCCTCCAACTAAATGGAAGGGCCAGTGCGACTTCAATGGAACAGTGTGCAACAACAAGCTCATTGGTGCACAAAATTTCGTAGGTGCAGAAGAAGGGAATATTACAGGAAGTCCATTTGATCTCGTTGGCCATGGCACCCACACTTCTAGCACCGCTGCTGGAAATTTTGTGGAAGGTGCCAGTGTGTTTGGAGAGGCCAATGGTACGGCTGTTGGCATGGCACCTTATGCTCACTTAGCAATGTACAAAGTCTGTTATGATGATGGTTGTAGCGAAGGCGATATCTTAGCTGCTCTTGATGTCGCTGTTGAAGATGGCTTGGACGTGCTTTCCCTTTCACTTGGAGGCCCCTCACTTCCTTTCTACGATGATGTGATTGCAATCGGTGCATTTGCAGCAATTAAAAAAGGAATATTTTTCAGTTGTGCGGCTGGAAATTCCGGAGCAGGAAATCGCTATAAGTCTTTGTCGAATGAGGCCCCATGGATTCTCACAGTTGGAGCAAGTACCACTGACAGAATACTTAAATCGGAAGATGAGAAGCTCAACACCTATGGCAGTATAATCGGAGATGCACTTGCTCCTAAGGTCGCTCACTTTTCTTCGCGAGGACCAAGCAGATCGATCCCGGGAATTTTGAAACCTGACATCATTGGACCTGGCGTTGACATCCTAGCAGCATGGCCTGAATCAGTAGACAATGCCACACTTCCTAATCCTAAGGCAACATTTAACATAATTTCAGGCACCTCAATGGCAACTCCTCACCTAAGCGGCATTGCGGCTTTGATCAAGAAATCACACCCTGACTGGTCTCCAGCTGCTATTAAATCTGCCATCATGACAACCGCTGATGTACTAAACCTCGCAGGCAAGCCCATCATTGACCAAGGGCTTTTCCCCGTGGACGTCTTTGCAATTGGTGCTGGCCATGTCAACCCTGCAAAGGCAAATGACCCAGGGCTCATCTTCGACATAAAACCAGAGGATTACTTTCCTTACTTGTGCGGTTTGAATTACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

509

Amino Acids

53.88

Weight (kDa)

5.0

Isoelectric Point (pI)

30.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 38 - 117 1.5e-10 Peptidase inhibitor I9
Peptidase_S8 PF00082 141 - 452 1.6e-45 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1413
Acc65I GGTACC 1 cut(s) 180
AccB1I GGYRCC 5 cut(s) 180, 635, 677, 721, 1247
AccI GTMKAC 1 cut(s) 1197
AccII CGCG 1 cut(s) 1108
AccIII TCCGGA 1 cut(s) 941
AciI CCGC 7 cut(s) 244, 654, 929, 1275, 1283, 1350, 1509
AclWI GGATC 2 cut(s) 236, 1120
AcoI YGGCCR 2 cut(s) 628, 1429
AcsI RAATTY 5 cut(s) 149, 574, 664, 937, 1134
AcuI CTGAAG 1 cut(s) 177
AcyI GRCGYC 1 cut(s) 1410
AfaI GTAC 5 cut(s) 182, 706, 746, 1009, 1359
AfiI CCNNNNNNNGG 1 cut(s) 1447
AgsI TTSAA 4 cut(s) 535, 812, 1141, 1516
AjiI CACGTC 1 cut(s) 826
AjnI CCWGG 2 cut(s) 1159, 1459
AluBI AGCT 5 cut(s) 269, 559, 791, 1045, 1322
AluI AGCT 5 cut(s) 269, 559, 791, 1045, 1322
Alw21I GWGCWC 1 cut(s) 571
Alw26I GTCTC 1 cut(s) 1320
Alw44I GTGCAC 1 cut(s) 567
AlwI GGATC 2 cut(s) 236, 1120
Ama87I CYCGRG 1 cut(s) 1129
Aor13HI TCCGGA 1 cut(s) 941
AoxI GGCC 8 cut(s) 138, 520, 628, 696, 847, 978, 1184, 1429
ApaLI GTGCAC 1 cut(s) 567
ApeKI GCWGC 7 cut(s) 20, 266, 656, 791, 896, 1178, 1322
ApoI RAATTY 5 cut(s) 149, 574, 664, 937, 1134
Asp718I GGTACC 1 cut(s) 180
AspS9I GGNCC 6 cut(s) 461, 520, 848, 979, 1112, 1157
AsuC2I CCSGG 2 cut(s) 1130, 1131
AsuHPI GGTGA 2 cut(s) 434, 1259
AvaI CYCGRG 1 cut(s) 1129
AvaII GGWCC 3 cut(s) 461, 1112, 1157
AxyI CCTNAGG 2 cut(s) 1086, 1221
BaeGI GKGCMC 1 cut(s) 571
BaeI ACNNNNGTAYC 2 cut(s) 696, 729
BalI TGGCCA 2 cut(s) 630, 1431
BanI GGYRCC 5 cut(s) 180, 635, 677, 721, 1247
BanII GRGCYC 1 cut(s) 1467
Bbv12I GWGCWC 1 cut(s) 571
BbvI GCAGC 7 cut(s) 32, 278, 643, 778, 908, 1190, 1309
BccI CCATC 5 cut(s) 419, 758, 809, 1346, 1388
BceAI ACGGC 2 cut(s) 236, 723
BcgI CGANNNNNNTGC 2 cut(s) 1048, 1082
BciT130I CCWGG 2 cut(s) 1161, 1461
BciVI GTATCC 3 cut(s) 386, 442, 505
BclI TGATCA 2 cut(s) 57, 1290
BcnI CCSGG 2 cut(s) 1130, 1131
BcoDI GTCTC 1 cut(s) 1320
BfaI CTAG 2 cut(s) 648, 1175
BfuI GTATCC 3 cut(s) 386, 442, 505
Bme1390I CCNGG 4 cut(s) 1130, 1131, 1161, 1461
Bme18I GGWCC 3 cut(s) 461, 1112, 1157
BmeT110I CYCGRG 1 cut(s) 1129
BmgBI CACGTC 1 cut(s) 826
BmgT120I GGNCC 6 cut(s) 461, 520, 848, 979, 1112, 1157
BmiI GGNNCC 9 cut(s) 182, 345, 463, 637, 679, 723, 850, 981, 1249
BmrFI CCNGG 4 cut(s) 1130, 1131, 1161, 1461
BmsI GCATC 1 cut(s) 1063
BpmI CTGGAG 1 cut(s) 1302
Bpu10I CCTNAGC 1 cut(s) 1271
BpuEI CTTGAG 1 cut(s) 353
BpuMI CCSGG 2 cut(s) 1130, 1131
Bsa29I ATCGAT 1 cut(s) 1124
BsaBI GATNNNNATC 1 cut(s) 881
BsaHI GRCGYC 1 cut(s) 1410
BsaI GGTCTC 1 cut(s) 1320
BsaJI CCNNGG 8 cut(s) 388, 631, 983, 1129, 1390, 1404, 1459, 1460
BsaWI WCCGGW 1 cut(s) 941
Bsc4I CCNNNNNNNGG 1 cut(s) 1447
Bse118I RCCGGY 1 cut(s) 136
Bse1I ACTGG 5 cut(s) 184, 262, 523, 681, 1316
Bse21I CCTNAGG 2 cut(s) 1086, 1221
Bse3DI GCAATG 4 cut(s) 30, 303, 747, 1278
Bse8I GATNNNNATC 1 cut(s) 881
BseAI TCCGGA 1 cut(s) 941
BseBI CCWGG 2 cut(s) 1161, 1461
BseCI ATCGAT 1 cut(s) 1124
BseDI CCNNGG 8 cut(s) 388, 631, 983, 1129, 1390, 1404, 1459, 1460
BseGI GGATG 2 cut(s) 469, 1170
BseJI GATNNNNATC 1 cut(s) 881
BseLI CCNNNNNNNGG 1 cut(s) 1447
BseMI GCAATG 4 cut(s) 30, 303, 747, 1278
BseNI ACTGG 5 cut(s) 184, 262, 523, 681, 1316
BseRI GAGGAG 4 cut(s) 107, 133, 492, 1254
BseSI GKGCMC 1 cut(s) 571
BseXI GCAGC 7 cut(s) 32, 278, 643, 778, 908, 1190, 1309
BsgI GTGCAG 2 cut(s) 39, 606
Bsh1236I CGCG 1 cut(s) 1108
BshFI GGCC 8 cut(s) 140, 522, 630, 698, 849, 980, 1186, 1431
BshNI GGYRCC 5 cut(s) 180, 635, 677, 721, 1247
BshVI ATCGAT 1 cut(s) 1124
BsiHKAI GWGCWC 1 cut(s) 571
BsiHKCI CYCGRG 1 cut(s) 1129
BsiSI CCGG 3 cut(s) 137, 942, 1130
BslI CCNNNNNNNGG 1 cut(s) 1447
BsmAI GTCTC 1 cut(s) 1320
BsnI GGCC 8 cut(s) 140, 522, 630, 698, 849, 980, 1186, 1431
Bso31I GGTCTC 1 cut(s) 1320
BsoBI CYCGRG 1 cut(s) 1129
Bsp1286I GDGCHC 2 cut(s) 571, 1467
Bsp13I TCCGGA 1 cut(s) 941
Bsp1407I TGTACA 1 cut(s) 744
Bsp143I GATC 7 cut(s) 57, 228, 466, 619, 1121, 1125, 1290
Bsp19I CCATGG 2 cut(s) 631, 983
Bsp68I TCGCGA 1 cut(s) 1108
BspACI CCGC 7 cut(s) 244, 654, 929, 1275, 1283, 1350, 1509
BspANI GGCC 8 cut(s) 140, 522, 630, 698, 849, 980, 1186, 1431
BspDI ATCGAT 1 cut(s) 1124
BspEI TCCGGA 1 cut(s) 941
BspFNI CGCG 1 cut(s) 1108
BspHI TCATGA 1 cut(s) 1341
BspLI GGNNCC 9 cut(s) 182, 345, 463, 637, 679, 723, 850, 981, 1249
BspPI GGATC 2 cut(s) 236, 1120
BspT107I GGYRCC 5 cut(s) 180, 635, 677, 721, 1247
BspTNI GGTCTC 1 cut(s) 1320
BsrDI GCAATG 4 cut(s) 30, 303, 747, 1278
BsrFI RCCGGY 1 cut(s) 136
BsrGI TGTACA 1 cut(s) 744
BsrI ACTGG 5 cut(s) 184, 262, 523, 681, 1316
BssAI RCCGGY 1 cut(s) 136
BssECI CCNNGG 8 cut(s) 388, 631, 983, 1129, 1390, 1404, 1459, 1460
BssMI GATC 7 cut(s) 57, 228, 466, 619, 1121, 1125, 1290
BssNI GRCGYC 1 cut(s) 1410
BssT1I CCWWGG 4 cut(s) 388, 631, 983, 1390
Bst2UI CCWGG 2 cut(s) 1161, 1461
Bst4CI ACNGT 5 cut(s) 176, 343, 422, 544, 997
BstACI GRCGYC 1 cut(s) 1410
BstAPI GCANNNNNTGC 3 cut(s) 728, 887, 1424
BstAUI TGTACA 1 cut(s) 744
BstC8I GCNNGC 4 cut(s) 138, 1372, 1376, 1429
BstDEI CTNAG 5 cut(s) 736, 787, 1086, 1221, 1271
BstDSI CCRYGG 3 cut(s) 631, 983, 1404
BstF5I GGATG 2 cut(s) 469, 1170
BstFNI CGCG 1 cut(s) 1108
BstKTI GATC 7 cut(s) 60, 231, 469, 622, 1124, 1128, 1293
BstMAI GTCTC 1 cut(s) 1320
BstMBI GATC 7 cut(s) 57, 228, 466, 619, 1121, 1125, 1290
BstMWI GCNNNNNNNGC 5 cut(s) 728, 737, 887, 896, 1424
BstNI CCWGG 2 cut(s) 1161, 1461
BstNSI RCATGY 1 cut(s) 32
BstSCI CCNGG 4 cut(s) 1128, 1129, 1159, 1459
BstSLI GKGCMC 1 cut(s) 571
BstUI CGCG 1 cut(s) 1108
BstV1I GCAGC 7 cut(s) 32, 278, 643, 778, 908, 1190, 1309
BstXI CCANNNNNNTGG 1 cut(s) 513
Bsu15I ATCGAT 1 cut(s) 1124
Bsu36I CCTNAGG 2 cut(s) 1086, 1221
BsuI GTATCC 3 cut(s) 386, 442, 505
BsuRI GGCC 8 cut(s) 140, 522, 630, 698, 849, 980, 1186, 1431
BsuTUI ATCGAT 1 cut(s) 1124
BtgI CCRYGG 3 cut(s) 631, 983, 1404
BtrI CACGTC 1 cut(s) 826
BtsCI GGATG 2 cut(s) 469, 1170
BtsI GCAGTG 1 cut(s) 253
BtsIMutI CAGTG 7 cut(s) 253, 255, 487, 530, 549, 688, 1011
BtuMI TCGCGA 1 cut(s) 1108
Cac8I GCNNGC 4 cut(s) 138, 1372, 1376, 1429
CciI TCATGA 1 cut(s) 1341
Cfr10I RCCGGY 1 cut(s) 136
Cfr13I GGNCC 6 cut(s) 461, 520, 848, 979, 1112, 1157
Cfr9I CCCGGG 1 cut(s) 1129
ClaI ATCGAT 1 cut(s) 1124
Csp6I GTAC 5 cut(s) 181, 705, 745, 1008, 1358
CviAII CATG 9 cut(s) 29, 61, 236, 632, 718, 984, 1182, 1342, 1433
CviQI GTAC 5 cut(s) 181, 705, 745, 1008, 1358
DdeI CTNAG 5 cut(s) 736, 787, 1086, 1221, 1271
DpnI GATC 7 cut(s) 59, 230, 468, 621, 1123, 1127, 1292
DpnII GATC 7 cut(s) 57, 228, 466, 619, 1121, 1125, 1290
EaeI YGGCCR 2 cut(s) 628, 1429
Eco130I CCWWGG 4 cut(s) 388, 631, 983, 1390
Eco24I GRGCYC 1 cut(s) 1467
Eco31I GGTCTC 1 cut(s) 1320
Eco32I GATATC 1 cut(s) 784
Eco47I GGWCC 3 cut(s) 461, 1112, 1157
Eco57I CTGAAG 1 cut(s) 177
Eco81I CCTNAGG 2 cut(s) 1086, 1221
Eco88I CYCGRG 1 cut(s) 1129
EcoO109I RGGNCCY 2 cut(s) 848, 979
EcoRII CCWGG 2 cut(s) 1159, 1459
EcoRV GATATC 1 cut(s) 784
EcoT14I CCWWGG 4 cut(s) 388, 631, 983, 1390
EcoT22I ATGCAT 2 cut(s) 30, 241
EcoT38I GRGCYC 1 cut(s) 1467
ErhI CCWWGG 4 cut(s) 388, 631, 983, 1390
FaeI CATG 9 cut(s) 32, 64, 239, 635, 721, 987, 1185, 1345, 1436
FatI CATG 9 cut(s) 28, 60, 235, 631, 717, 983, 1181, 1341, 1432
FbaI TGATCA 2 cut(s) 57, 1290
FblI GTMKAC 1 cut(s) 1197
FokI GGATG 2 cut(s) 456, 1157
FriOI GRGCYC 1 cut(s) 1467
FspBI CTAG 2 cut(s) 648, 1175
GsuI CTGGAG 1 cut(s) 1302
HaeIII GGCC 8 cut(s) 140, 522, 630, 698, 849, 980, 1186, 1431
HapII CCGG 3 cut(s) 137, 942, 1130
Hin1I GRCGYC 1 cut(s) 1410
Hin1II CATG 9 cut(s) 32, 64, 239, 635, 721, 987, 1185, 1345, 1436
HincII GTYRAC 2 cut(s) 1168, 1438
HindII GTYRAC 2 cut(s) 1168, 1438
HinfI GANTC 4 cut(s) 161, 441, 988, 1190
HpaII CCGG 3 cut(s) 137, 942, 1130
HphI GGTGA 2 cut(s) 434, 1259
Hpy166II GTNNAC 5 cut(s) 569, 1168, 1198, 1408, 1438
Hpy188I TCNGA 5 cut(s) 57, 157, 166, 1033, 1070
Hpy188III TCNNGA 8 cut(s) 128, 332, 368, 797, 942, 1107, 1294, 1342
Hpy8I GTNNAC 5 cut(s) 569, 1168, 1198, 1408, 1438
HpyAV CCTTC 7 cut(s) 305, 482, 483, 511, 587, 668, 770
HpyCH4III ACNGT 5 cut(s) 176, 343, 422, 544, 997
HpyCH4IV ACGT 2 cut(s) 825, 1410
HpyF10VI GCNNNNNNNGC 5 cut(s) 728, 737, 887, 896, 1424
HpyF3I CTNAG 5 cut(s) 736, 787, 1086, 1221, 1271
HpySE526I ACGT 2 cut(s) 825, 1410
Hsp92I GRCGYC 1 cut(s) 1410
Hsp92II CATG 9 cut(s) 32, 64, 239, 635, 721, 987, 1185, 1345, 1436
Kpn2I TCCGGA 1 cut(s) 941
KpnI GGTACC 1 cut(s) 184
KroI GCCGGC 1 cut(s) 136
KroNI GCCGGC 1 cut(s) 138
Ksp22I TGATCA 2 cut(s) 57, 1290
Kzo9I GATC 7 cut(s) 57, 228, 466, 619, 1121, 1125, 1290
LmnI GCTCC 4 cut(s) 94, 944, 1001, 1087
Lsp1109I GCAGC 7 cut(s) 32, 278, 643, 778, 908, 1190, 1309
LweI GCATC 1 cut(s) 1063
MaeI CTAG 2 cut(s) 648, 1175
MaeII ACGT 2 cut(s) 825, 1410
MaeIII GTNAC 3 cut(s) 253, 381, 482
MalI GATC 7 cut(s) 59, 230, 468, 621, 1123, 1127, 1292
MboI GATC 7 cut(s) 57, 228, 466, 619, 1121, 1125, 1290
MboII GAAGA 7 cut(s) 32, 170, 602, 824, 1046, 1095, 1462
MfeI CAATTG 1 cut(s) 1419
MhlI GDGCHC 2 cut(s) 571, 1467
MlsI TGGCCA 2 cut(s) 630, 1431
MluNI TGGCCA 2 cut(s) 630, 1431
MlyI GAGTC 1 cut(s) 435
MmeI TCCRAC 2 cut(s) 530, 979
Mox20I TGGCCA 2 cut(s) 630, 1431
Mph1103I ATGCAT 2 cut(s) 30, 241
MroI TCCGGA 1 cut(s) 941
MroNI GCCGGC 1 cut(s) 136
MscI TGGCCA 2 cut(s) 630, 1431
MseI TTAA 6 cut(s) 215, 903, 1026, 1233, 1329, 1525
MslI CAYNNNNRTG 1 cut(s) 1253
Msp20I TGGCCA 2 cut(s) 630, 1431
MspA1I CMGCKG 3 cut(s) 656, 1322, 1352
MspI CCGG 3 cut(s) 137, 942, 1130
MspR9I CCNGG 4 cut(s) 1130, 1131, 1161, 1461
MunI CAATTG 1 cut(s) 1419
MvaI CCWGG 2 cut(s) 1161, 1461
MvnI CGCG 1 cut(s) 1108
MwoI GCNNNNNNNGC 5 cut(s) 728, 737, 887, 896, 1424
NaeI GCCGGC 1 cut(s) 138
NciI CCSGG 2 cut(s) 1130, 1131
NcoI CCATGG 2 cut(s) 631, 983
NdeII GATC 7 cut(s) 57, 228, 466, 619, 1121, 1125, 1290
NgoMIV GCCGGC 1 cut(s) 136
NlaIII CATG 9 cut(s) 32, 64, 239, 635, 721, 987, 1185, 1345, 1436
NlaIV GGNNCC 9 cut(s) 182, 345, 463, 637, 679, 723, 850, 981, 1249
NmuCI GTSAC 2 cut(s) 253, 482
NruI TCGCGA 1 cut(s) 1108
NsiI ATGCAT 2 cut(s) 30, 241
NspI RCATGY 1 cut(s) 32
PagI TCATGA 1 cut(s) 1341
PasI CCCWGGG 1 cut(s) 1460
PdiI GCCGGC 1 cut(s) 138
PfeI GAWTC 3 cut(s) 161, 988, 1190
PleI GAGTC 1 cut(s) 435
PpsI GAGTC 1 cut(s) 435
Psp6I CCWGG 2 cut(s) 1159, 1459
PspGI CCWGG 2 cut(s) 1159, 1459
PspN4I GGNNCC 9 cut(s) 182, 345, 463, 637, 679, 723, 850, 981, 1249
PspPI GGNCC 6 cut(s) 461, 520, 848, 979, 1112, 1157
PvuII CAGCTG 1 cut(s) 1322
RruI TCGCGA 1 cut(s) 1108
RsaI GTAC 5 cut(s) 182, 706, 746, 1009, 1359
RsaNI GTAC 5 cut(s) 181, 705, 745, 1008, 1358
RseI CAYNNNNRTG 1 cut(s) 1253
SaqAI TTAA 6 cut(s) 215, 903, 1026, 1233, 1329, 1525
Sau3AI GATC 7 cut(s) 57, 228, 466, 619, 1121, 1125, 1290
Sau96I GGNCC 6 cut(s) 461, 520, 848, 979, 1112, 1157
SchI GAGTC 1 cut(s) 435
ScrFI CCNGG 4 cut(s) 1130, 1131, 1161, 1461
SduI GDGCHC 2 cut(s) 571, 1467
SfaNI GCATC 1 cut(s) 1063
SinI GGWCC 3 cut(s) 461, 1112, 1157
SmaI CCCGGG 1 cut(s) 1131
SmiMI CAYNNNNRTG 1 cut(s) 1253
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
SsiI CCGC 7 cut(s) 244, 654, 929, 1275, 1283, 1350, 1509
SspI AATATT 2 cut(s) 601, 915
SspMI CTAG 2 cut(s) 648, 1175
StyD4I CCNGG 4 cut(s) 1128, 1129, 1159, 1459
StyI CCWWGG 4 cut(s) 388, 631, 983, 1390
TaaI ACNGT 5 cut(s) 176, 343, 422, 544, 997
TaiI ACGT 2 cut(s) 828, 1413
TaqI TCGA 4 cut(s) 129, 971, 1124, 1473
TatI WGTACW 2 cut(s) 744, 1357
TauI GCSGC 3 cut(s) 932, 1278, 1286
TfiI GAWTC 3 cut(s) 161, 988, 1190
Tru1I TTAA 6 cut(s) 215, 903, 1026, 1233, 1329, 1525
Tru9I TTAA 6 cut(s) 215, 903, 1026, 1233, 1329, 1525
TscAI CASTG 7 cut(s) 253, 262, 487, 530, 549, 688, 1018
TseFI GTSAC 2 cut(s) 253, 482
TseI GCWGC 7 cut(s) 20, 266, 656, 791, 896, 1178, 1322
Tsp45I GTSAC 2 cut(s) 253, 482
TspDTI ATGAA 2 cut(s) 224, 465
TspGWI ACGGA 1 cut(s) 411
TspMI CCCGGG 1 cut(s) 1129
TspRI CASTG 7 cut(s) 253, 262, 487, 530, 549, 688, 1018
VneI GTGCAC 1 cut(s) 567
VpaK11BI GGWCC 3 cut(s) 461, 1112, 1157
XapI RAATTY 5 cut(s) 149, 574, 664, 937, 1134
XceI RCATGY 1 cut(s) 32
XmaI CCCGGG 1 cut(s) 1129
XmiI GTMKAC 1 cut(s) 1197
XspI CTAG 2 cut(s) 648, 1175
ZraI GACGTC 1 cut(s) 1411
Zsp2I ATGCAT 2 cut(s) 30, 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.