MD00G1069500.v1.1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000302
Physical Location & Seq
Forward (+)
13595016 .. 13597292
2277 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1069500.v1.1.491

Sequence Viewer

Length: 2277 bp
ATGGAGGGCAAAAGGTTTGCTATAGTGTTGCAGATTATTAATCTTCTTGGCTTCATTTTCGTGTTTGATCCATCACTAGTAGCTGCAGATGAGGAGCAAAATGGCATGCAAACTTATATTGTTTGGGTAGAAAAGCCGCCGGTTTCTCTGGAACCTTCTGCACTGTCCCATGAAGACTTGGAGAGGTGGTATCGATCATTTTTGCCAGAAAATAGGATTGAAAGCTCGAAGAAGATGCAAATTGTCCATGCATACCACAATGTGGCAACTGGATTTGCAGCAAAGCTGACACCAGAGGAAGTGAAAGCGATGGAAAAGAAACAAGGATTTGTTTCTGCTCATCCGGAAGCTATTCTGCCTTTGCACACAACTCATAGTCCCAACTTCTTGGGTTTGCAGCAAGGATCGGGATTTTGGAAAGGAGCAAATTATGGTAAAGGTGTGATCATTGGAGTTTTGGATACTGGGATTTCACCCGATCATCCCTCATTTAGTGACAAAGGAGTGCCTCCTCCTCCAGCTAAATGGAAAGGCAAATGTGAGTTTGATGGGGCAGTATGTAATAACAAACTTATTGGTGCAAGAAGTTTCGTAGACGGGCAACCTGCAGGAGGTCCTCCATTTGACGGAGAAGGCCACGGGACACACACAGCCAGCACAGCTGGTGGAAATTTTGTGAAACGCGCCGCAGTGTTTGAAATGGCCAAAGGCACAGCGGTTGGCATGGCGCCATATGCTCATTTGGCAATCTACAAAGTATGCTCAATTTTGGGTTGCTTCGAGGGTGACATTTTGGCTGCTTTTGATGCTGCTGTTGATGATGGCGTGGATGTGATCTCGCTCTCAGTTGGGGGTCCCTCTGGTCCTTTTTATACAGATGGGATTGCAATTGGCGCATTTGGAGCAATGCAAAGGGGAATTTTTGTTAGCTGCTCTGCCGGAAACTCTGGTCCTTCCTATTCTAGTTTATCAAATGTGGCCCCATGGATTCTCACCGTCGGCGCCAGCACTATTGACAGAAGCATAAGAGCAACGACACGTCTTGGGAATAAGAAGGCATACAATGGGGAATCCCTCTTCCAGCCTAAGAATTTTAACTCAAATTCAAAATTAATGTTACCCCTTGTGTATGCAGGCGCAATTGGCAACACTTCTTCCCCCTCTTGCGAACATGGTACGCTTGAAAATGTTCGAGGGAAACTAGTGCTGTGCGAGAGAGGTGGAATGAGTGGAAGAGTCGACAAAGGGGCAGAAGTGAAAAGAGCTGGTGGGGCTGCCATGATTCTCGTGAACCAAGGCATTGATGGCTATAGCACATCAGCTGACCCTCATGTGCTTCCCGCAACACATGTGAGTTATGCCGCAGGGTTGAAAATTAAGTCTTATATAAAATCAACCTCAAAACCAACAGCGACAATTTTATTCAAAGGCACTGTTATTGGAAATTCGCAAGCTCCCAGTGTCGCTTCGTTTTCATCAAGAGGACCAAGCGTTCCAAGTCCTGGGATTTTGAAACCGGACATCATTGGTCCTGGTGTTAGCATCTTAGCTGCATGGCCTGTTCCAGTGGACAATGCCATAAAGTCCAAAGCAACATTCAACATAATTTCGGGTACTTCAATGTCATGCCCTCACCTAAGTGGCATTGCAGCTCTGCTCAAGAGTTTCCACCCCGACTGGTCTCCAGCTGCGATTAAATCCGCAATCATGACAACCGCAGATGTACATAACCTCGGAGGTAAGTCCATAGTTGATCAAACACTTAGGCCAGCAGACATTTTTGCAATAGGTGCAGGCCATGTTAACCCTACAAAAGCAAATGACCCCGGGCTCATATACGACACACAGCCGAAGGATTATATCCGTTACTTGTGCGGTTTGAAATACACAGAGAAACAGATACAGATCATCACCCAACAAAAAGTAAAATGCTCAGAAGTAGGATCCATATCAGAAGCACAGTTAAATTACCCTACATTTTCTCTCATAGTAGGTTCCAATAACAAGACTCGGTCTCAACTTTACACAAGGACAGTGAAGAATGTCGGGCCGGCTAATTCGACTTACAAGCTGGAAATTTTAGCACCACAAAAGATGAATGTGGATGTCAGTCCACCGGAGCTTAGATTCCTGAAGGCCAACCAGATCATTACGTACCGTGTGAAGTTCACGGCACAAAAGGGTGCAGGGAAGGAAGGTGTATCATTTGGTCAGGGATATTTGAGATGGGTTTCTGATAAGTATAAGGTGACTAGCCCTATAGCTGTGCTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

759

Amino Acids

80.62

Weight (kDa)

9.14

Isoelectric Point (pI)

34.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 38 - 122 5.7e-10 Peptidase inhibitor I9
Peptidase_S8 PF00082 145 - 576 7.9e-45 Subtilase family
PA PF02225 373 - 459 3.4e-14 PA domain
fn3_6 PF17766 654 - 756 1.8e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 613
AccB1I GGYRCC 2 cut(s) 727, 1001
AccB7I CCANNNNNTGG 2 cut(s) 262, 1502
AccI GTMKAC 2 cut(s) 594, 1239
AccII CGCG 1 cut(s) 684
AccIII TCCGGA 1 cut(s) 343
AciI CCGC 8 cut(s) 137, 687, 716, 1341, 1362, 1701, 1716, 1875
AclWI GGATC 4 cut(s) 62, 412, 1938, 1951
AcoI YGGCCR 1 cut(s) 702
AcsI RAATTY 6 cut(s) 670, 918, 1090, 1102, 1444, 2076
AcuI CTGAAG 1 cut(s) 2153
AcyI GRCGYC 2 cut(s) 728, 1002
AdeI CACNNNGTG 1 cut(s) 262
AfaI GTAC 4 cut(s) 1177, 1615, 1725, 2156
AfiI CCNNNNNNNGG 4 cut(s) 262, 611, 626, 1502
AflIII ACRYGT 2 cut(s) 1037, 1348
AhlI ACTAGT 2 cut(s) 76, 1201
AjiI CACGTC 1 cut(s) 1040
AjnI CCWGG 2 cut(s) 1501, 1531
AjuI GAANNNNNNNTTGG 2 cut(s) 1580, 1612
AleI CACNNNNGTG 1 cut(s) 1638
AloI GAACNNNNNNTCC 2 cut(s) 1476, 1508
Alw21I GWGCWC 1 cut(s) 2271
Alw26I GTCTC 2 cut(s) 1686, 2019
AlwI GGATC 4 cut(s) 62, 412, 1938, 1951
Ama87I CYCGRG 1 cut(s) 1826
Aor13HI TCCGGA 1 cut(s) 343
AoxI GGCC 8 cut(s) 634, 702, 978, 1556, 1766, 1795, 2048, 2136
ApoI RAATTY 6 cut(s) 670, 918, 1090, 1102, 1444, 2076
AseI ATTAAT 2 cut(s) 39, 1112
Asp700I GAANNNNTTC 2 cut(s) 351, 1188
AspLEI GCGC 5 cut(s) 686, 730, 896, 1004, 1139
AspS9I GGNCC 8 cut(s) 614, 854, 863, 950, 979, 1484, 1529, 2048
AsuC2I CCSGG 2 cut(s) 1827, 1828
AsuHPI GGTGA 6 cut(s) 465, 797, 985, 1625, 1903, 2260
AvaI CYCGRG 1 cut(s) 1826
AvaII GGWCC 6 cut(s) 614, 854, 863, 950, 1484, 1529
BalI TGGCCA 1 cut(s) 704
BamHI GGATCC 1 cut(s) 1943
BanI GGYRCC 2 cut(s) 727, 1001
BanII GRGCYC 1 cut(s) 1833
BauI CACGAG 1 cut(s) 1286
BbsI GAAGAC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 2271
BccI CCATC 7 cut(s) 79, 304, 542, 815, 872, 1298, 2220
BceAI ACGGC 1 cut(s) 2187
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BciT130I CCWGG 2 cut(s) 1503, 1533
BciVI GTATCC 1 cut(s) 454
BclI TGATCA 2 cut(s) 444, 1753
BcnI CCSGG 2 cut(s) 1827, 1828
BcoDI GTCTC 2 cut(s) 1686, 2019
BcuI ACTAGT 2 cut(s) 76, 1201
BfaI CTAG 4 cut(s) 77, 963, 1202, 2253
BfmI CTRYAG 5 cut(s) 21, 84, 606, 1309, 2259
BfoI RGCGCY 2 cut(s) 731, 1005
BfuAI ACCTGC 1 cut(s) 613
BfuI GTATCC 1 cut(s) 454
Bme1390I CCNGG 4 cut(s) 1503, 1533, 1827, 1828
Bme18I GGWCC 6 cut(s) 614, 854, 863, 950, 1484, 1529
BmeT110I CYCGRG 1 cut(s) 1826
BmgBI CACGTC 1 cut(s) 1040
BmgT120I GGNCC 8 cut(s) 614, 854, 863, 950, 979, 1484, 1529, 2048
BmiI GGNNCC 8 cut(s) 153, 729, 855, 856, 981, 1003, 1945, 1996
BmrFI CCNGG 4 cut(s) 1503, 1533, 1827, 1828
BmrI ACTGGG 2 cut(s) 474, 1452
BmsI GCATC 3 cut(s) 225, 796, 1551
BmuI ACTGGG 2 cut(s) 474, 1452
BpiI GAAGAC 1 cut(s) 180
BpmI CTGGAG 2 cut(s) 501, 1668
BpuEI CTTGAG 1 cut(s) 1643
BpuMI CCSGG 2 cut(s) 1827, 1828
Bsa29I ATCGAT 1 cut(s) 193
BsaAI YACGTR 1 cut(s) 2154
BsaBI GATNNNNATC 2 cut(s) 1904, 1948
BsaHI GRCGYC 2 cut(s) 728, 1002
BsaI GGTCTC 2 cut(s) 1686, 2019
BsaJI CCNNGG 7 cut(s) 637, 983, 1294, 1502, 1732, 1825, 1826
BsaWI WCCGGW 3 cut(s) 343, 1516, 2116
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 4 cut(s) 262, 611, 626, 1502
Bse118I RCCGGY 2 cut(s) 139, 2050
Bse1I ACTGG 5 cut(s) 274, 469, 1458, 1565, 1682
Bse3DI GCAATG 2 cut(s) 912, 1644
Bse8I GATNNNNATC 2 cut(s) 1904, 1948
BseAI TCCGGA 1 cut(s) 343
BseBI CCWGG 2 cut(s) 1503, 1533
BseCI ATCGAT 1 cut(s) 193
BseDI CCNNGG 7 cut(s) 637, 983, 1294, 1502, 1732, 1825, 1826
BseGI GGATG 4 cut(s) 340, 481, 835, 2110
BseJI GATNNNNATC 2 cut(s) 1904, 1948
BseLI CCNNNNNNNGG 4 cut(s) 262, 611, 626, 1502
BseMI GCAATG 2 cut(s) 912, 1644
BseMII CTCAG 2 cut(s) 858, 1947
BseNI ACTGG 5 cut(s) 274, 469, 1458, 1565, 1682
BseRI GAGGAG 3 cut(s) 107, 501, 504
BsgI GTGCAG 3 cut(s) 144, 1812, 2205
Bsh1236I CGCG 1 cut(s) 684
BshFI GGCC 8 cut(s) 636, 704, 980, 1558, 1768, 1797, 2050, 2138
BshNI GGYRCC 2 cut(s) 727, 1001
BshVI ATCGAT 1 cut(s) 193
BsiHKAI GWGCWC 1 cut(s) 2271
BsiHKCI CYCGRG 1 cut(s) 1826
BsiSI CCGG 7 cut(s) 140, 344, 939, 1517, 1827, 2051, 2117
BslFI GGGAC 4 cut(s) 151, 363, 655, 840
BslI CCNNNNNNNGG 4 cut(s) 262, 611, 626, 1502
BsmAI GTCTC 2 cut(s) 1686, 2019
BsmFI GGGAC 4 cut(s) 151, 363, 655, 840
BsnI GGCC 8 cut(s) 636, 704, 980, 1558, 1768, 1797, 2050, 2138
Bso31I GGTCTC 2 cut(s) 1686, 2019
BsoBI CYCGRG 1 cut(s) 1826
Bsp1286I GDGCHC 2 cut(s) 1833, 2271
Bsp13I TCCGGA 1 cut(s) 343
Bsp1407I TGTACA 1 cut(s) 1723
Bsp19I CCATGG 1 cut(s) 983
BspACI CCGC 8 cut(s) 137, 687, 716, 1341, 1362, 1701, 1716, 1875
BspANI GGCC 8 cut(s) 636, 704, 980, 1558, 1768, 1797, 2050, 2138
BspCNI CTCAG 2 cut(s) 857, 1946
BspDI ATCGAT 1 cut(s) 193
BspEI TCCGGA 1 cut(s) 343
BspFNI CGCG 1 cut(s) 684
BspHI TCATGA 1 cut(s) 1707
BspLI GGNNCC 8 cut(s) 153, 729, 855, 856, 981, 1003, 1945, 1996
BspMAI CTGCAG 2 cut(s) 88, 610
BspMI ACCTGC 1 cut(s) 613
BspPI GGATC 4 cut(s) 62, 412, 1938, 1951
BspT107I GGYRCC 2 cut(s) 727, 1001
BspTNI GGTCTC 2 cut(s) 1686, 2019
BsrDI GCAATG 2 cut(s) 912, 1644
BsrFI RCCGGY 2 cut(s) 139, 2050
BsrGI TGTACA 1 cut(s) 1723
BsrI ACTGG 5 cut(s) 274, 469, 1458, 1565, 1682
BssAI RCCGGY 2 cut(s) 139, 2050
BssECI CCNNGG 7 cut(s) 637, 983, 1294, 1502, 1732, 1825, 1826
BssNI GRCGYC 2 cut(s) 728, 1002
BssSI CACGAG 1 cut(s) 1286
BssT1I CCWWGG 2 cut(s) 983, 1294
Bst2BI CACGAG 1 cut(s) 1286
Bst2UI CCWGG 2 cut(s) 1503, 1533
Bst4CI ACNGT 6 cut(s) 165, 997, 1435, 1962, 2035, 2159
Bst6I CTCTTC 2 cut(s) 1082, 1228
BstACI GRCGYC 2 cut(s) 728, 1002
BstAPI GCANNNNNTGC 1 cut(s) 1790
BstAUI TGTACA 1 cut(s) 1723
BstBAI YACGTR 1 cut(s) 2154
BstC8I GCNNGC 8 cut(s) 107, 655, 1006, 1135, 1452, 1770, 1795, 2052
BstDEI CTNAG 7 cut(s) 844, 1086, 1546, 1637, 1763, 1933, 2123
BstDSI CCRYGG 2 cut(s) 637, 983
BstENI CCTNNNNNAGG 1 cut(s) 609
BstF5I GGATG 4 cut(s) 340, 481, 835, 2110
BstFNI CGCG 1 cut(s) 684
BstH2I RGCGCY 2 cut(s) 731, 1005
BstHHI GCGC 5 cut(s) 686, 730, 896, 1004, 1139
BstMAI GTCTC 2 cut(s) 1686, 2019
BstNI CCWGG 2 cut(s) 1503, 1533
BstNSI RCATGY 2 cut(s) 109, 1352
BstSCI CCNGG 4 cut(s) 1501, 1531, 1825, 1826
BstSFI CTRYAG 5 cut(s) 21, 84, 606, 1309, 2259
BstSNI TACGTA 1 cut(s) 2154
BstUI CGCG 1 cut(s) 684
BstV2I GAAGAC 1 cut(s) 180
BstX2I RGATCY 1 cut(s) 1943
BstXI CCANNNNNNTGG 2 cut(s) 388, 525
BstYI RGATCY 1 cut(s) 1943
Bsu15I ATCGAT 1 cut(s) 193
BsuI GTATCC 1 cut(s) 454
BsuRI GGCC 8 cut(s) 636, 704, 980, 1558, 1768, 1797, 2050, 2138
BsuTUI ATCGAT 1 cut(s) 193
BtgI CCRYGG 2 cut(s) 637, 983
BtgZI GCGATG 1 cut(s) 323
BtrI CACGTC 1 cut(s) 1040
BtsCI GGATG 4 cut(s) 340, 481, 835, 2110
BtsI GCAGTG 1 cut(s) 696
BtsIMutI CAGTG 6 cut(s) 161, 696, 1431, 1465, 1572, 2040
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 8 cut(s) 107, 655, 1006, 1135, 1452, 1770, 1795, 2052
CciI TCATGA 1 cut(s) 1707
CfoI GCGC 5 cut(s) 686, 730, 896, 1004, 1139
Cfr10I RCCGGY 2 cut(s) 139, 2050
Cfr13I GGNCC 8 cut(s) 614, 854, 863, 950, 979, 1484, 1529, 2048
Cfr9I CCCGGG 1 cut(s) 1826
ClaI ATCGAT 1 cut(s) 193
Csp6I GTAC 4 cut(s) 1176, 1614, 1724, 2155
CviQI GTAC 4 cut(s) 1176, 1614, 1724, 2155
DdeI CTNAG 7 cut(s) 844, 1086, 1546, 1637, 1763, 1933, 2123
DinI GGCGCC 2 cut(s) 729, 1003
DraIII CACNNNGTG 1 cut(s) 262
EaeI YGGCCR 1 cut(s) 702
Eam1104I CTCTTC 2 cut(s) 1082, 1228
EarI CTCTTC 2 cut(s) 1082, 1228
Eco105I TACGTA 1 cut(s) 2154
Eco130I CCWWGG 2 cut(s) 983, 1294
Eco24I GRGCYC 1 cut(s) 1833
Eco31I GGTCTC 2 cut(s) 1686, 2019
Eco47I GGWCC 6 cut(s) 614, 854, 863, 950, 1484, 1529
Eco57I CTGAAG 1 cut(s) 2153
Eco88I CYCGRG 1 cut(s) 1826
EcoNI CCTNNNNNAGG 1 cut(s) 609
EcoO109I RGGNCCY 2 cut(s) 614, 854
EcoRII CCWGG 2 cut(s) 1501, 1531
EcoT14I CCWWGG 2 cut(s) 983, 1294
EcoT22I ATGCAT 1 cut(s) 253
EcoT38I GRGCYC 1 cut(s) 1833
EgeI GGCGCC 2 cut(s) 729, 1003
EheI GGCGCC 2 cut(s) 729, 1003
ErhI CCWWGG 2 cut(s) 983, 1294
FaqI GGGAC 4 cut(s) 151, 363, 655, 840
FauI CCCGC 1 cut(s) 1348
FauNDI CATATG 1 cut(s) 733
FbaI TGATCA 2 cut(s) 444, 1753
FblI GTMKAC 2 cut(s) 594, 1239
FokI GGATG 4 cut(s) 327, 468, 842, 2117
FriOI GRGCYC 1 cut(s) 1833
FspBI CTAG 4 cut(s) 77, 963, 1202, 2253
GlaI GCGC 5 cut(s) 685, 729, 895, 1003, 1138
GsuI CTGGAG 2 cut(s) 501, 1668
HaeII RGCGCY 2 cut(s) 731, 1005
HaeIII GGCC 8 cut(s) 636, 704, 980, 1558, 1768, 1797, 2050, 2138
HapII CCGG 7 cut(s) 140, 344, 939, 1517, 1827, 2051, 2117
HhaI GCGC 5 cut(s) 686, 730, 896, 1004, 1139
Hin1I GRCGYC 2 cut(s) 728, 1002
Hin6I GCGC 5 cut(s) 684, 728, 894, 1002, 1137
HinP1I GCGC 5 cut(s) 684, 728, 894, 1002, 1137
HincII GTYRAC 2 cut(s) 1240, 1804
HindII GTYRAC 2 cut(s) 1240, 1804
HinfI GANTC 6 cut(s) 988, 1070, 1236, 1282, 2008, 2127
HpaI GTTAAC 1 cut(s) 1804
HpaII CCGG 7 cut(s) 140, 344, 939, 1517, 1827, 2051, 2117
HphI GGTGA 6 cut(s) 465, 797, 985, 1625, 1903, 2260
Hpy166II GTNNAC 7 cut(s) 595, 1240, 1291, 1570, 1804, 2114, 2169
Hpy188I TCNGA 4 cut(s) 1736, 1936, 1954, 2236
Hpy188III TCNNGA 8 cut(s) 149, 344, 408, 1288, 1479, 1660, 1708, 2131
Hpy8I GTNNAC 7 cut(s) 595, 1240, 1291, 1570, 1804, 2114, 2169
Hpy99I CGWCG 1 cut(s) 1001
HpyAV CCTTC 8 cut(s) 165, 626, 963, 1048, 1846, 2128, 2185, 2189
HpyCH4III ACNGT 6 cut(s) 165, 997, 1435, 1962, 2035, 2159
HpyCH4IV ACGT 2 cut(s) 1039, 2153
HpyF3I CTNAG 7 cut(s) 844, 1086, 1546, 1637, 1763, 1933, 2123
HpySE526I ACGT 2 cut(s) 1039, 2153
Hsp92I GRCGYC 2 cut(s) 728, 1002
HspAI GCGC 5 cut(s) 684, 728, 894, 1002, 1137
KasI GGCGCC 2 cut(s) 727, 1001
KflI GGGWCCC 1 cut(s) 854
Kpn2I TCCGGA 1 cut(s) 343
KroI GCCGGC 1 cut(s) 2050
KroNI GCCGGC 1 cut(s) 2052
Ksp22I TGATCA 2 cut(s) 444, 1753
KspAI GTTAAC 1 cut(s) 1804
LmnI GCTCC 5 cut(s) 94, 422, 902, 1459, 2119
LweI GCATC 3 cut(s) 225, 796, 1551
MaeI CTAG 4 cut(s) 77, 963, 1202, 2253
MaeII ACGT 2 cut(s) 1039, 2153
MaeIII GTNAC 5 cut(s) 494, 785, 1116, 1865, 2248
MboII GAAGA 8 cut(s) 35, 185, 241, 244, 1069, 1146, 1245, 2050
MfeI CAATTG 2 cut(s) 888, 1140
MflI RGATCY 1 cut(s) 1943
MhlI GDGCHC 2 cut(s) 1833, 2271
MlsI TGGCCA 1 cut(s) 704
MluNI TGGCCA 1 cut(s) 704
Mly113I GGCGCC 2 cut(s) 728, 1002
MlyI GAGTC 2 cut(s) 1245, 2002
Mox20I TGGCCA 1 cut(s) 704
Mph1103I ATGCAT 1 cut(s) 253
MroI TCCGGA 1 cut(s) 343
MroNI GCCGGC 1 cut(s) 2050
MroXI GAANNNNTTC 2 cut(s) 351, 1188
MscI TGGCCA 1 cut(s) 704
MseI TTAA 8 cut(s) 39, 1095, 1112, 1377, 1695, 1803, 1964, 2275
MslI CAYNNNNRTG 2 cut(s) 59, 1638
Msp20I TGGCCA 1 cut(s) 704
MspA1I CMGCKG 4 cut(s) 662, 716, 1322, 1688
MspI CCGG 7 cut(s) 140, 344, 939, 1517, 1827, 2051, 2117
MspR9I CCNGG 4 cut(s) 1503, 1533, 1827, 1828
MunI CAATTG 2 cut(s) 888, 1140
MvaI CCWGG 2 cut(s) 1503, 1533
MvnI CGCG 1 cut(s) 684
NaeI GCCGGC 1 cut(s) 2052
NarI GGCGCC 2 cut(s) 728, 1002
NciI CCSGG 2 cut(s) 1827, 1828
NcoI CCATGG 1 cut(s) 983
NdeI CATATG 1 cut(s) 733
NgoMIV GCCGGC 1 cut(s) 2050
NlaIV GGNNCC 8 cut(s) 153, 729, 855, 856, 981, 1003, 1945, 1996
NmuCI GTSAC 3 cut(s) 494, 785, 2248
NsiI ATGCAT 1 cut(s) 253
NspI RCATGY 2 cut(s) 109, 1352
OliI CACNNNNGTG 1 cut(s) 1638
PaeI GCATGC 1 cut(s) 109
PagI TCATGA 1 cut(s) 1707
PciI ACATGT 1 cut(s) 1348
PdiI GCCGGC 1 cut(s) 2052
PdmI GAANNNNTTC 2 cut(s) 351, 1188
PfeI GAWTC 4 cut(s) 988, 1070, 1282, 2127
PflFI GACNNNGTC 1 cut(s) 2011
PflMI CCANNNNNTGG 2 cut(s) 262, 1502
PleI GAGTC 2 cut(s) 1244, 2002
PluTI GGCGCC 2 cut(s) 731, 1005
PpsI GAGTC 2 cut(s) 1244, 2002
Ppu21I YACGTR 1 cut(s) 2154
PpuMI RGGWCCY 2 cut(s) 614, 854
PscI ACATGT 1 cut(s) 1348
PshBI ATTAAT 2 cut(s) 39, 1112
Psp5II RGGWCCY 2 cut(s) 614, 854
Psp6I CCWGG 2 cut(s) 1501, 1531
PspGI CCWGG 2 cut(s) 1501, 1531
PspN4I GGNNCC 8 cut(s) 153, 729, 855, 856, 981, 1003, 1945, 1996
PspPI GGNCC 8 cut(s) 614, 854, 863, 950, 979, 1484, 1529, 2048
PspPPI RGGWCCY 2 cut(s) 614, 854
PstI CTGCAG 2 cut(s) 88, 610
PsuI RGATCY 1 cut(s) 1943
PsyI GACNNNGTC 1 cut(s) 2011
PvuII CAGCTG 3 cut(s) 662, 1322, 1688
RsaI GTAC 4 cut(s) 1177, 1615, 1725, 2156
RsaNI GTAC 4 cut(s) 1176, 1614, 1724, 2155
RseI CAYNNNNRTG 2 cut(s) 59, 1638
SalI GTCGAC 1 cut(s) 1238
SaqAI TTAA 8 cut(s) 39, 1095, 1112, 1377, 1695, 1803, 1964, 2275
Sau96I GGNCC 8 cut(s) 614, 854, 863, 950, 979, 1484, 1529, 2048
SbfI CCTGCAGG 1 cut(s) 610
SchI GAGTC 2 cut(s) 1245, 2002
ScrFI CCNGG 4 cut(s) 1503, 1533, 1827, 1828
SdaI CCTGCAGG 1 cut(s) 610
SduI GDGCHC 2 cut(s) 1833, 2271
SfaNI GCATC 3 cut(s) 225, 796, 1551
SfcI CTRYAG 5 cut(s) 21, 84, 606, 1309, 2259
SfoI GGCGCC 2 cut(s) 729, 1003
SinI GGWCC 6 cut(s) 614, 854, 863, 950, 1484, 1529
SmaI CCCGGG 1 cut(s) 1828
SmiMI CAYNNNNRTG 2 cut(s) 59, 1638
SmlI CTYRAG 1 cut(s) 1658
SmoI CTYRAG 1 cut(s) 1658
SnaBI TACGTA 1 cut(s) 2154
SpeI ACTAGT 2 cut(s) 76, 1201
SphI GCATGC 1 cut(s) 109
Sse8387I CCTGCAGG 1 cut(s) 610
SsiI CCGC 8 cut(s) 137, 687, 716, 1341, 1362, 1701, 1716, 1875
SspDI GGCGCC 2 cut(s) 727, 1001
SspMI CTAG 4 cut(s) 77, 963, 1202, 2253
StyD4I CCNGG 4 cut(s) 1501, 1531, 1825, 1826
StyI CCWWGG 2 cut(s) 983, 1294
TaaI ACNGT 6 cut(s) 165, 997, 1435, 1962, 2035, 2159
TaiI ACGT 2 cut(s) 1042, 2156
TaqI TCGA 6 cut(s) 193, 227, 780, 1192, 1239, 2060
TaqII GACCGA 1 cut(s) 2001
TatI WGTACW 1 cut(s) 1723
TauI GCSGC 3 cut(s) 139, 689, 1364
TfiI GAWTC 4 cut(s) 988, 1070, 1282, 2127
Tru1I TTAA 8 cut(s) 39, 1095, 1112, 1377, 1695, 1803, 1964, 2275
Tru9I TTAA 8 cut(s) 39, 1095, 1112, 1377, 1695, 1803, 1964, 2275
TscAI CASTG 6 cut(s) 168, 696, 1438, 1465, 1572, 2040
TseFI GTSAC 3 cut(s) 494, 785, 2248
Tsp45I GTSAC 3 cut(s) 494, 785, 2248
TspDTI ATGAA 4 cut(s) 43, 186, 1464, 2111
TspGWI ACGGA 2 cut(s) 642, 1853
TspMI CCCGGG 1 cut(s) 1826
TspRI CASTG 6 cut(s) 168, 696, 1438, 1465, 1572, 2040
Tth111I GACNNNGTC 1 cut(s) 2011
Van91I CCANNNNNTGG 2 cut(s) 262, 1502
VpaK11BI GGWCC 6 cut(s) 614, 854, 863, 950, 1484, 1529
VspI ATTAAT 2 cut(s) 39, 1112
XagI CCTNNNNNAGG 1 cut(s) 609
XapI RAATTY 6 cut(s) 670, 918, 1090, 1102, 1444, 2076
XceI RCATGY 2 cut(s) 109, 1352
XcmI CCANNNNNNNNNTGG 1 cut(s) 1301
XmaI CCCGGG 1 cut(s) 1826
XmiI GTMKAC 2 cut(s) 594, 1239
XmnI GAANNNNTTC 2 cut(s) 351, 1188
XspI CTAG 4 cut(s) 77, 963, 1202, 2253
Zsp2I ATGCAT 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.