MD00G1069300.v1.1

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000302
Physical Location & Seq
Reverse (-)
13575181 .. 13576496
1316 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1069300.v1.1.491

Sequence Viewer

Length: 600 bp
ATGGCACTAGAGGTAGTTTTGAGGAGGAGCGAAAGGTGCAATTGTATAAGGGAAGGTGATATGTTAGCTGGTCTTGATGCCACTGTTGAAGATGGCATGGACGTGCTCTCCCTCTCACTTGAAGGCCTCTCATTACTGTTCTACGATGATGTGATTGCAATCGATGCTCAACACCTATGGCACTATTACAGAGATGCGCTTGCTCCCAAGGTCGCTCACTCTTCATCAAGAGGACCAAGCATTCCAAGCCCTGGAATTTTGAAACCCGACATCATTGCACCCGGTGTCGACATCCTAGCAGCATGGCCTGAATTAATGGACAATGCTATAGTTCCTAATCCTAAGGCCACACTTAACATAATTTCTGGTACCTCAATGGCAACTCCTCACCTAAGTGGCATTGCAACTCTGATCAAGAAATCACACCCTGACTGGTCTCCAGCTGCTATTAAATATGCCATCATGACAACCACTGATGTACTAAACCTCGTAGGCACACCCATCGTTGATCAAAGGTTAAACCCGTGGACGTCTTTGCAACTGGTGGAGGCCGTGTCAACCCTGCAAAGGCAAATGACCCGGGGCTTATCTTCGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

200

Amino Acids

21.54

Weight (kDa)

5.25

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 66 - 159 3.5e-17 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 533
Acc65I GGTACC 1 cut(s) 368
AccB1I GGYRCC 1 cut(s) 368
AccB7I CCANNNNNTGG 1 cut(s) 251
AccI GTMKAC 1 cut(s) 288
AcsI RAATTY 1 cut(s) 255
AcyI GRCGYC 1 cut(s) 530
AdeI CACNNNGTG 1 cut(s) 284
AfaI GTAC 2 cut(s) 370, 480
AfiI CCNNNNNNNGG 2 cut(s) 251, 567
AgsI TTSAA 3 cut(s) 89, 122, 262
AjiI CACGTC 1 cut(s) 103
AjnI CCWGG 1 cut(s) 250
AleI CACNNNNGTG 1 cut(s) 393
AluBI AGCT 2 cut(s) 68, 443
AluI AGCT 2 cut(s) 68, 443
Alw21I GWGCWC 1 cut(s) 108
Alw26I GTCTC 1 cut(s) 441
Ama87I CYCGRG 1 cut(s) 579
AoxI GGCC 4 cut(s) 124, 305, 345, 549
ApeKI GCWGC 2 cut(s) 299, 443
ApoI RAATTY 1 cut(s) 255
AseI ATTAAT 1 cut(s) 314
Asp718I GGTACC 1 cut(s) 368
AspLEI GCGC 1 cut(s) 199
AspS9I GGNCC 1 cut(s) 233
AsuC2I CCSGG 3 cut(s) 282, 580, 581
AsuHPI GGTGA 2 cut(s) 68, 380
AvaI CYCGRG 1 cut(s) 579
AvaII GGWCC 1 cut(s) 233
AxyI CCTNAGG 1 cut(s) 342
BanI GGYRCC 1 cut(s) 368
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 2 cut(s) 311, 430
BccI CCATC 3 cut(s) 86, 467, 509
BceAI ACGGC 1 cut(s) 536
BcgI CGANNNNNNTGC 4 cut(s) 257, 291, 484, 518
BciT130I CCWGG 1 cut(s) 252
BclI TGATCA 2 cut(s) 411, 508
BcnI CCSGG 3 cut(s) 282, 580, 581
BcoDI GTCTC 1 cut(s) 441
BfaI CTAG 2 cut(s) 8, 296
BfmI CTRYAG 1 cut(s) 327
BisI GCNGC 2 cut(s) 300, 444
BlsI GCNGC 2 cut(s) 301, 445
Bme1390I CCNGG 4 cut(s) 252, 282, 580, 581
Bme18I GGWCC 1 cut(s) 233
BmeT110I CYCGRG 1 cut(s) 579
BmgBI CACGTC 1 cut(s) 103
BmgT120I GGNCC 1 cut(s) 233
BmiI GGNNCC 1 cut(s) 370
BmrFI CCNGG 4 cut(s) 252, 282, 580, 581
BmsI GCATC 3 cut(s) 67, 154, 184
BpmI CTGGAG 1 cut(s) 423
BpuMI CCSGG 3 cut(s) 282, 580, 581
Bsa29I ATCGAT 1 cut(s) 162
BsaBI GATNNNNATC 1 cut(s) 158
BsaHI GRCGYC 1 cut(s) 530
BsaI GGTCTC 1 cut(s) 441
BsaJI CCNNGG 5 cut(s) 207, 250, 524, 579, 580
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 539, 569
Bsc4I CCNNNNNNNGG 2 cut(s) 251, 567
Bse1I ACTGG 2 cut(s) 437, 546
Bse21I CCTNAGG 1 cut(s) 342
Bse3DI GCAATG 2 cut(s) 273, 399
Bse8I GATNNNNATC 1 cut(s) 158
BseBI CCWGG 1 cut(s) 252
BseCI ATCGAT 1 cut(s) 162
BseDI CCNNGG 5 cut(s) 207, 250, 524, 579, 580
BseGI GGATG 1 cut(s) 291
BseJI GATNNNNATC 1 cut(s) 158
BseLI CCNNNNNNNGG 2 cut(s) 251, 567
BseMI GCAATG 2 cut(s) 273, 399
BseNI ACTGG 2 cut(s) 437, 546
BseRI GAGGAG 3 cut(s) 37, 40, 375
BseXI GCAGC 2 cut(s) 311, 430
BshFI GGCC 4 cut(s) 126, 307, 347, 551
BshNI GGYRCC 1 cut(s) 368
BshVI ATCGAT 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 108
BsiHKCI CYCGRG 1 cut(s) 579
BsiSI CCGG 2 cut(s) 282, 580
BslI CCNNNNNNNGG 2 cut(s) 251, 567
BsmAI GTCTC 1 cut(s) 441
BsmI GAATGC 1 cut(s) 240
BsnI GGCC 4 cut(s) 126, 307, 347, 551
Bso31I GGTCTC 1 cut(s) 441
BsoBI CYCGRG 1 cut(s) 579
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 2 cut(s) 411, 508
BspANI GGCC 4 cut(s) 126, 307, 347, 551
BspDI ATCGAT 1 cut(s) 162
BspHI TCATGA 1 cut(s) 462
BspLI GGNNCC 1 cut(s) 370
BspT107I GGYRCC 1 cut(s) 368
BspTNI GGTCTC 1 cut(s) 441
BsrDI GCAATG 2 cut(s) 273, 399
BsrI ACTGG 2 cut(s) 437, 546
BssECI CCNNGG 5 cut(s) 207, 250, 524, 579, 580
BssMI GATC 2 cut(s) 411, 508
BssNI GRCGYC 1 cut(s) 530
BssT1I CCWWGG 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 2 cut(s) 85, 138
Bst6I CTCTTC 1 cut(s) 226
BstACI GRCGYC 1 cut(s) 530
BstAPI GCANNNNNTGC 1 cut(s) 164
BstC8I GCNNGC 1 cut(s) 201
BstDEI CTNAG 2 cut(s) 342, 392
BstDSI CCRYGG 1 cut(s) 524
BstF5I GGATG 1 cut(s) 291
BstHHI GCGC 1 cut(s) 199
BstKTI GATC 2 cut(s) 414, 511
BstMAI GTCTC 1 cut(s) 441
BstMBI GATC 2 cut(s) 411, 508
BstMWI GCNNNNNNNGC 3 cut(s) 36, 164, 246
BstNI CCWGG 1 cut(s) 252
BstSCI CCNGG 4 cut(s) 250, 280, 578, 579
BstSFI CTRYAG 1 cut(s) 327
BstV1I GCAGC 2 cut(s) 311, 430
Bsu15I ATCGAT 1 cut(s) 162
Bsu36I CCTNAGG 1 cut(s) 342
BsuRI GGCC 4 cut(s) 126, 307, 347, 551
BsuTUI ATCGAT 1 cut(s) 162
BtgI CCRYGG 1 cut(s) 524
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 1 cut(s) 291
BtsIMutI CAGTG 2 cut(s) 81, 471
Cac8I GCNNGC 1 cut(s) 201
CciI TCATGA 1 cut(s) 462
CfoI GCGC 1 cut(s) 199
Cfr13I GGNCC 1 cut(s) 233
Cfr9I CCCGGG 1 cut(s) 579
ClaI ATCGAT 1 cut(s) 162
Csp6I GTAC 2 cut(s) 369, 479
CviAII CATG 3 cut(s) 97, 303, 463
CviJI RGCY 8 cut(s) 68, 126, 249, 307, 347, 443, 551, 585
CviKI_1 RGCY 8 cut(s) 68, 126, 249, 307, 347, 443, 551, 585
CviQI GTAC 2 cut(s) 369, 479
DdeI CTNAG 2 cut(s) 342, 392
DpnI GATC 2 cut(s) 413, 510
DpnII GATC 2 cut(s) 411, 508
DraIII CACNNNGTG 1 cut(s) 284
Eam1104I CTCTTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 207
Eco147I AGGCCT 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 441
Eco47I GGWCC 1 cut(s) 233
Eco81I CCTNAGG 1 cut(s) 342
Eco88I CYCGRG 1 cut(s) 579
EcoRII CCWGG 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 207
FaeI CATG 3 cut(s) 100, 306, 466
FatI CATG 3 cut(s) 96, 302, 462
FbaI TGATCA 2 cut(s) 411, 508
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 2 cut(s) 300, 444
FokI GGATG 1 cut(s) 278
Fsp4HI GCNGC 2 cut(s) 300, 444
FspBI CTAG 2 cut(s) 8, 296
GlaI GCGC 1 cut(s) 198
GluI GCNGC 2 cut(s) 300, 444
GsuI CTGGAG 1 cut(s) 423
HaeIII GGCC 4 cut(s) 126, 307, 347, 551
HapII CCGG 2 cut(s) 282, 580
HhaI GCGC 1 cut(s) 199
Hin1I GRCGYC 1 cut(s) 530
Hin1II CATG 3 cut(s) 100, 306, 466
Hin6I GCGC 1 cut(s) 197
HinP1I GCGC 1 cut(s) 197
HincII GTYRAC 2 cut(s) 289, 558
HindII GTYRAC 2 cut(s) 289, 558
HpaII CCGG 2 cut(s) 282, 580
HphI GGTGA 2 cut(s) 68, 380
Hpy166II GTNNAC 3 cut(s) 289, 528, 558
Hpy188I TCNGA 1 cut(s) 411
Hpy188III TCNNGA 4 cut(s) 74, 228, 415, 463
Hpy8I GTNNAC 3 cut(s) 289, 528, 558
HpyAV CCTTC 2 cut(s) 47, 116
HpyCH4III ACNGT 2 cut(s) 85, 138
HpyCH4IV ACGT 2 cut(s) 102, 530
HpyCH4V TGCA 6 cut(s) 39, 158, 278, 404, 538, 565
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 164, 246
HpyF3I CTNAG 2 cut(s) 342, 392
HpySE526I ACGT 2 cut(s) 102, 530
Hsp92I GRCGYC 1 cut(s) 530
Hsp92II CATG 3 cut(s) 100, 306, 466
HspAI GCGC 1 cut(s) 197
KpnI GGTACC 1 cut(s) 372
Ksp22I TGATCA 2 cut(s) 411, 508
Kzo9I GATC 2 cut(s) 411, 508
LmnI GCTCC 2 cut(s) 27, 208
Lsp1109I GCAGC 2 cut(s) 311, 430
LweI GCATC 3 cut(s) 67, 154, 184
MaeI CTAG 2 cut(s) 8, 296
MaeII ACGT 2 cut(s) 102, 530
MalI GATC 2 cut(s) 413, 510
MboI GATC 2 cut(s) 411, 508
MboII GAAGA 3 cut(s) 101, 213, 582
MfeI CAATTG 1 cut(s) 40
MhlI GDGCHC 1 cut(s) 108
MluCI AATT 4 cut(s) 40, 255, 311, 360
MseI TTAA 4 cut(s) 314, 354, 450, 518
MslI CAYNNNNRTG 2 cut(s) 101, 393
MspA1I CMGCKG 1 cut(s) 443
MspI CCGG 2 cut(s) 282, 580
MspR9I CCNGG 4 cut(s) 252, 282, 580, 581
MunI CAATTG 1 cut(s) 40
Mva1269I GAATGC 1 cut(s) 240
MvaI CCWGG 1 cut(s) 252
MwoI GCNNNNNNNGC 3 cut(s) 36, 164, 246
NciI CCSGG 3 cut(s) 282, 580, 581
NdeII GATC 2 cut(s) 411, 508
NlaIII CATG 3 cut(s) 100, 306, 466
NlaIV GGNNCC 1 cut(s) 370
OliI CACNNNNGTG 1 cut(s) 393
PagI TCATGA 1 cut(s) 462
PceI AGGCCT 1 cut(s) 126
PctI GAATGC 1 cut(s) 240
PflMI CCANNNNNTGG 1 cut(s) 251
PkrI GCNGC 2 cut(s) 301, 445
PshBI ATTAAT 1 cut(s) 314
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 1 cut(s) 370
PspPI GGNCC 1 cut(s) 233
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 2 cut(s) 370, 480
RsaNI GTAC 2 cut(s) 369, 479
RseI CAYNNNNRTG 2 cut(s) 101, 393
SalI GTCGAC 1 cut(s) 287
SaqAI TTAA 4 cut(s) 314, 354, 450, 518
SatI GCNGC 2 cut(s) 300, 444
Sau3AI GATC 2 cut(s) 411, 508
Sau96I GGNCC 1 cut(s) 233
ScrFI CCNGG 4 cut(s) 252, 282, 580, 581
SduI GDGCHC 1 cut(s) 108
SfaNI GCATC 3 cut(s) 67, 154, 184
SfcI CTRYAG 1 cut(s) 327
SinI GGWCC 1 cut(s) 233
SmaI CCCGGG 1 cut(s) 581
SmiMI CAYNNNNRTG 2 cut(s) 101, 393
Sse9I AATT 4 cut(s) 40, 255, 311, 360
SseBI AGGCCT 1 cut(s) 126
SspMI CTAG 2 cut(s) 8, 296
StuI AGGCCT 1 cut(s) 126
StyD4I CCNGG 4 cut(s) 250, 280, 578, 579
StyI CCWWGG 1 cut(s) 207
TaaI ACNGT 2 cut(s) 85, 138
TaiI ACGT 2 cut(s) 105, 533
TaqI TCGA 3 cut(s) 162, 288, 593
TasI AATT 4 cut(s) 40, 255, 311, 360
TatI WGTACW 1 cut(s) 478
Tru1I TTAA 4 cut(s) 314, 354, 450, 518
Tru9I TTAA 4 cut(s) 314, 354, 450, 518
TscAI CASTG 2 cut(s) 88, 478
TseI GCWGC 2 cut(s) 299, 443
TspDTI ATGAA 1 cut(s) 213
TspMI CCCGGG 1 cut(s) 579
TspRI CASTG 2 cut(s) 88, 478
Van91I CCANNNNNTGG 1 cut(s) 251
VpaK11BI GGWCC 1 cut(s) 233
VspI ATTAAT 1 cut(s) 314
XapI RAATTY 1 cut(s) 255
XmaI CCCGGG 1 cut(s) 579
XmiI GTMKAC 1 cut(s) 288
XspI CTAG 2 cut(s) 8, 296
ZraI GACGTC 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.