Rroxscaffold_3G00256260

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000302
Physical Location & Seq
Forward (+)
50634984 .. 50635984
1001 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00256260.1

Sequence Viewer

Length: 573 bp
ATGCCAGCCACTATTTCGAAACCTTGTCATCCAAGTGACAACAAGCCAACCCTGGCGCCCATTGTTGATGAAAGACATGCTCCAGCAGACATCTTTGCCACTGGTGCAGGCCATGTCAAACCTTCAAAGGCAAATGACCCTGGGCTCATCTACGACCTGAACCCTGAAGATTACATCCCCTACTTGTGCGGTTTGAATTATACAGATGATCAGATACTGATAATCACCCAAGAAACAGTGAAATGCTCTCAAGTTGTAGCCATACCAGCAACACAGCTAAACTACCCTTCATTTACTATAATAATACTCGACCCAACTCAGTCTCAAGATTACACAAGGACGGTGACGAATGTTGATCCAGCTAATTCCACTTACAAGTTGGATCTTTTGGTACCGAAAACAATAGGCATGACTGTGAGTCCTTCAGTGCTCACATTCACAGAGGTCGGCCAGACCATCACATACCATGTCAAGTTTGTCCCACAAAATGGTATGGGGAAGGATGGACCATTCCATCAGGGATATCTGAGATGGGTTTCTGATAAGCATTCTGTTACTAGCCCCATAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

190

Amino Acids

20.84

Weight (kDa)

5.38

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 92 - 189 5.5e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 391
AccB1I GGYRCC 2 cut(s) 55, 391
AccB7I CCANNNNNTGG 1 cut(s) 488
AciI CCGC 1 cut(s) 189
AclWI GGATC 2 cut(s) 350, 390
AcoI YGGCCR 1 cut(s) 448
AcuI CTGAAG 2 cut(s) 186, 408
AcyI GRCGYC 1 cut(s) 56
AfaI GTAC 1 cut(s) 393
AfiI CCNNNNNNNGG 1 cut(s) 488
AgsI TTSAA 2 cut(s) 126, 196
AhdI GACNNNNNGTC 1 cut(s) 417
AjnI CCWGG 2 cut(s) 51, 139
AluBI AGCT 2 cut(s) 277, 362
AluI AGCT 2 cut(s) 277, 362
Alw21I GWGCWC 1 cut(s) 432
Alw26I GTCTC 1 cut(s) 327
AlwI GGATC 2 cut(s) 350, 390
AlwNI CAGNNNCTG 1 cut(s) 217
AoxI GGCC 2 cut(s) 109, 448
Asp718I GGTACC 1 cut(s) 391
AspLEI GCGC 1 cut(s) 58
AspS9I GGNCC 1 cut(s) 506
AsuHPI GGTGA 2 cut(s) 217, 355
AsuII TTCGAA 1 cut(s) 17
AvaII GGWCC 1 cut(s) 506
BanI GGYRCC 2 cut(s) 55, 391
BanII GRGCYC 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 432
BccI CCATC 4 cut(s) 464, 497, 522, 525
BciT130I CCWGG 2 cut(s) 53, 141
BclI TGATCA 1 cut(s) 208
BcoDI GTCTC 1 cut(s) 327
BfaI CTAG 1 cut(s) 558
BfoI RGCGCY 1 cut(s) 59
Bme1390I CCNGG 2 cut(s) 53, 141
Bme18I GGWCC 1 cut(s) 506
BmeRI GACNNNNNGTC 1 cut(s) 417
BmgT120I GGNCC 1 cut(s) 506
BmiI GGNNCC 2 cut(s) 57, 393
BmrFI CCNGG 2 cut(s) 53, 141
BpmI CTGGAG 1 cut(s) 66
Bpu14I TTCGAA 1 cut(s) 17
BpuEI CTTGAG 2 cut(s) 234, 309
BsaHI GRCGYC 1 cut(s) 56
BsaJI CCNNGG 3 cut(s) 51, 139, 140
Bsc4I CCNNNNNNNGG 1 cut(s) 488
Bse1I ACTGG 1 cut(s) 106
BseBI CCWGG 2 cut(s) 53, 141
BseDI CCNNGG 3 cut(s) 51, 139, 140
BseGI GGATG 3 cut(s) 28, 174, 508
BseLI CCNNNNNNNGG 1 cut(s) 488
BseMII CTCAG 2 cut(s) 332, 518
BseNI ACTGG 1 cut(s) 106
BsgI GTGCAG 1 cut(s) 126
BshFI GGCC 2 cut(s) 111, 450
BshNI GGYRCC 2 cut(s) 55, 391
BsiHKAI GWGCWC 1 cut(s) 432
BslFI GGGAC 1 cut(s) 464
BslI CCNNNNNNNGG 1 cut(s) 488
BsmAI GTCTC 1 cut(s) 327
BsmFI GGGAC 1 cut(s) 464
BsmI GAATGC 1 cut(s) 547
BsnI GGCC 2 cut(s) 111, 450
Bsp119I TTCGAA 1 cut(s) 17
Bsp1286I GDGCHC 2 cut(s) 147, 432
Bsp143I GATC 3 cut(s) 208, 355, 382
BspACI CCGC 1 cut(s) 189
BspANI GGCC 2 cut(s) 111, 450
BspCNI CTCAG 2 cut(s) 331, 519
BspLI GGNNCC 2 cut(s) 57, 393
BspPI GGATC 2 cut(s) 350, 390
BspT104I TTCGAA 1 cut(s) 17
BspT107I GGYRCC 2 cut(s) 55, 391
BsrI ACTGG 1 cut(s) 106
BssECI CCNNGG 3 cut(s) 51, 139, 140
BssMI GATC 3 cut(s) 208, 355, 382
BssNI GRCGYC 1 cut(s) 56
Bst2UI CCWGG 2 cut(s) 53, 141
Bst4CI ACNGT 3 cut(s) 238, 343, 415
BstACI GRCGYC 1 cut(s) 56
BstBI TTCGAA 1 cut(s) 17
BstC8I GCNNGC 2 cut(s) 6, 109
BstDEI CTNAG 2 cut(s) 318, 527
BstF5I GGATG 3 cut(s) 28, 174, 508
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 3 cut(s) 211, 358, 385
BstMAI GTCTC 1 cut(s) 327
BstMBI GATC 3 cut(s) 208, 355, 382
BstMWI GCNNNNNNNGC 2 cut(s) 104, 266
BstNI CCWGG 2 cut(s) 53, 141
BstNSI RCATGY 1 cut(s) 80
BstSCI CCNGG 2 cut(s) 51, 139
BstX2I RGATCY 1 cut(s) 382
BstYI RGATCY 1 cut(s) 382
BsuRI GGCC 2 cut(s) 111, 450
BtsCI GGATG 3 cut(s) 28, 174, 508
BtsIMutI CAGTG 3 cut(s) 99, 243, 432
Cac8I GCNNGC 2 cut(s) 6, 109
CaiI CAGNNNCTG 1 cut(s) 217
CfoI GCGC 1 cut(s) 58
Cfr13I GGNCC 1 cut(s) 506
Csp6I GTAC 1 cut(s) 392
CviAII CATG 4 cut(s) 77, 113, 409, 467
CviJI RGCY 9 cut(s) 8, 46, 111, 145, 260, 277, 362, 450, 561
CviKI_1 RGCY 9 cut(s) 8, 46, 111, 145, 260, 277, 362, 450, 561
CviQI GTAC 1 cut(s) 392
DdeI CTNAG 2 cut(s) 318, 527
DinI GGCGCC 1 cut(s) 57
DpnI GATC 3 cut(s) 210, 357, 384
DpnII GATC 3 cut(s) 208, 355, 382
DriI GACNNNNNGTC 1 cut(s) 417
EaeI YGGCCR 1 cut(s) 448
Eam1105I GACNNNNNGTC 1 cut(s) 417
Eco24I GRGCYC 1 cut(s) 147
Eco32I GATATC 1 cut(s) 524
Eco47I GGWCC 1 cut(s) 506
Eco57I CTGAAG 2 cut(s) 186, 408
EcoRII CCWGG 2 cut(s) 51, 139
EcoRV GATATC 1 cut(s) 524
EcoT38I GRGCYC 1 cut(s) 147
EgeI GGCGCC 1 cut(s) 57
EheI GGCGCC 1 cut(s) 57
FaeI CATG 4 cut(s) 80, 116, 412, 470
FaqI GGGAC 1 cut(s) 464
FatI CATG 4 cut(s) 76, 112, 408, 466
FbaI TGATCA 1 cut(s) 208
FokI GGATG 3 cut(s) 15, 161, 515
FriOI GRGCYC 1 cut(s) 147
FspBI CTAG 1 cut(s) 558
GlaI GCGC 1 cut(s) 57
GsuI CTGGAG 1 cut(s) 66
HaeII RGCGCY 1 cut(s) 59
HaeIII GGCC 2 cut(s) 111, 450
HhaI GCGC 1 cut(s) 58
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 4 cut(s) 80, 116, 412, 470
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HinfI GANTC 1 cut(s) 418
HphI GGTGA 2 cut(s) 217, 355
Hpy188I TCNGA 3 cut(s) 213, 528, 541
Hpy188III TCNNGA 1 cut(s) 326
HpyAV CCTTC 4 cut(s) 132, 297, 432, 493
HpyCH4III ACNGT 3 cut(s) 238, 343, 415
HpyCH4V TGCA 1 cut(s) 107
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 266
HpyF3I CTNAG 2 cut(s) 318, 527
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 4 cut(s) 80, 116, 412, 470
HspAI GCGC 1 cut(s) 56
KasI GGCGCC 1 cut(s) 55
KpnI GGTACC 1 cut(s) 395
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 3 cut(s) 208, 355, 382
LmnI GCTCC 1 cut(s) 85
MaeI CTAG 1 cut(s) 558
MaeIII GTNAC 3 cut(s) 35, 343, 553
MalI GATC 3 cut(s) 210, 357, 384
MboI GATC 3 cut(s) 208, 355, 382
MboII GAAGA 1 cut(s) 179
MflI RGATCY 1 cut(s) 382
MhlI GDGCHC 2 cut(s) 147, 432
MluCI AATT 2 cut(s) 196, 364
Mly113I GGCGCC 1 cut(s) 56
MlyI GAGTC 1 cut(s) 427
MmeI TCCRAC 1 cut(s) 360
MnlI CCTC 1 cut(s) 436
MslI CAYNNNNRTG 2 cut(s) 33, 413
MspR9I CCNGG 2 cut(s) 53, 141
Mva1269I GAATGC 1 cut(s) 547
MvaI CCWGG 2 cut(s) 53, 141
MwoI GCNNNNNNNGC 2 cut(s) 104, 266
NarI GGCGCC 1 cut(s) 56
NdeII GATC 3 cut(s) 208, 355, 382
NlaIII CATG 4 cut(s) 80, 116, 412, 470
NlaIV GGNNCC 2 cut(s) 57, 393
NmuCI GTSAC 2 cut(s) 35, 343
NspI RCATGY 1 cut(s) 80
NspV TTCGAA 1 cut(s) 17
PasI CCCWGGG 1 cut(s) 140
PctI GAATGC 1 cut(s) 547
PflMI CCANNNNNTGG 1 cut(s) 488
PleI GAGTC 1 cut(s) 426
PluTI GGCGCC 1 cut(s) 59
PpsI GAGTC 1 cut(s) 426
Psp6I CCWGG 2 cut(s) 51, 139
PspGI CCWGG 2 cut(s) 51, 139
PspN4I GGNNCC 2 cut(s) 57, 393
PspPI GGNCC 1 cut(s) 506
PstNI CAGNNNCTG 1 cut(s) 217
PsuI RGATCY 1 cut(s) 382
RsaI GTAC 1 cut(s) 393
RsaNI GTAC 1 cut(s) 392
RseI CAYNNNNRTG 2 cut(s) 33, 413
Sau3AI GATC 3 cut(s) 208, 355, 382
Sau96I GGNCC 1 cut(s) 506
SchI GAGTC 1 cut(s) 427
ScrFI CCNGG 2 cut(s) 53, 141
SduI GDGCHC 2 cut(s) 147, 432
SetI ASST 6 cut(s) 25, 124, 159, 279, 364, 447
SfoI GGCGCC 1 cut(s) 57
SfuI TTCGAA 1 cut(s) 17
SinI GGWCC 1 cut(s) 506
SmiMI CAYNNNNRTG 2 cut(s) 33, 413
SmlI CTYRAG 2 cut(s) 249, 324
SmoI CTYRAG 2 cut(s) 249, 324
Sse9I AATT 2 cut(s) 196, 364
SsiI CCGC 1 cut(s) 189
SspDI GGCGCC 1 cut(s) 55
SspMI CTAG 1 cut(s) 558
StyD4I CCNGG 2 cut(s) 51, 139
TaaI ACNGT 3 cut(s) 238, 343, 415
TaqI TCGA 2 cut(s) 17, 309
TasI AATT 2 cut(s) 196, 364
TscAI CASTG 3 cut(s) 106, 243, 432
TseFI GTSAC 2 cut(s) 35, 343
Tsp45I GTSAC 2 cut(s) 35, 343
TspDTI ATGAA 2 cut(s) 84, 279
TspRI CASTG 3 cut(s) 106, 243, 432
Van91I CCANNNNNTGG 1 cut(s) 488
VpaK11BI GGWCC 1 cut(s) 506
XceI RCATGY 1 cut(s) 80
XcmI CCANNNNNNNNNTGG 1 cut(s) 376
XspI CTAG 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.