RLG00000029001

Subtilisin-like serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000302
Physical Location & Seq
Forward (+)
31236119 .. 31243927
7809 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029001

Sequence Viewer

Length: 1776 bp
ATGGTTCACTCATACAGCAATGTGGCCACTGGCTTTGCAGCGAAACTGACACTAGAGGAAGTGAAAGCAATGGAAAAGAAAGATGGGTTCGTGCATGCTCATCCAGAGAGGTTTCTGCCTTTGCACACTAGTCGTAGTCCAAACTTCCTGGGGCAGCAACAAGGGTTCGGACTTTGGAAAAGGACTAATTATGGTGAAGATCATCCCTCATTTAGTGATGAACGAGTGCCACCTCCTCCGGTGAAATGGAAAGGCAAATGCGAGTTCAAGTTCAATCGTACGACAGTGTGCAACAACAAGCTTATTGGTGCAAGAAATTTCCAAGGTGGGAAAGAAACAAAAGAAGTCTCTCCATTTGGTGAAGAAGGCCATGGCACTCTCACTTCTAGCATAGCTGCTGGAAACTTTGTGAAAGGCGCCAGCGTGTTTGGAATGGCAAATGGCACAGCATCTGGCATTGCACCTTATGCTCACTTGGCCATCTACAAGGTCTGCACCGAGGCAGGTTGTGCCGAAAGTGATATGCTAGCAGCTCTTGACACTGCTGTTGAGGACGGTGTGGATGTGCTCTCACTGTCCATAAGTTTTGCTTCCTCGGTTCCTTTCCATCAAGATGGGGTTGCTATCAGTGTGTTTGGAGTAATTCGAAAGAGAATTTTTGTCAGCTGTTCAGCTGGAAATTCTGGCCTTTTCTATAGTTCCATGTCAAACGAGGCCCCTTGGATTCTCACCGTTGGAGCAAGCACCATTGACAGAAAAATCAGCTCAATAGCACAGCTTGGAGATTCTCCAAAATATTTTTACCCTGGTGAATCTTTATTCAATCCTAAAATTTTTAGCACTTCACCATTGCTACCTCTTGTTTATCTAGGTGCAAATCCCAATAACCAAACTGAGTTCTGTGCCTTGGGATCTCTTGTAAATGTTGAAGGGAAAGTAGTGGTGTGTGAAAGAGGTGGAGGAGTGGGAAGAACTGCCAAAGGGGAAGAAGTGAAAAAAGCAGGAGGTGCTGCCATGATTCTAGTGAACCAAATCACTGATGACGATAGCACCTTAGCTGATGCTCATGTACTTCCAGCAACAAATGTTGGTTTTCAAGCAGGTTTGAGTATCAAAACCTATATAAAATCAACAACAACACCTATAGCCACAGTCTATTTTCCACGCACTGAAATAGGAGATACACTTGCTCTAATGGTTGCTTCTTTCTCATCAAGAGGACCGAGCATAGCTAGCCCTGGAATTTTGAAACCTGACATCATTGGTCATGGTCTTAATATCCTCGCTGCATGGCCTGTTTCGGTGGATAATGTCACAACATTTGACATTCTTTCAGGTAATAATCCTAAACACAATAATCTGAAACTTGATTTGATTTCAGGTACCTCAGTGTCATGCCCACACCTAAGTGGCATTGCAGCCTTAATCAAGAGTTCACACCCAGACTGGTCACCTGCTGCCATTAAATCTGCCATTATGACAACTGCTGATATGCTAAATCTCGAAGGCACACCAATTGTTGATAGATCAAATATCCCAGCAGACATCTACGTACAGCACTGCCTCAGGCCATACAATCAAATAGAGATGATCACCCAACAAAGAGTGGACTGCTCTGAGGTAGGAGTCACAGCTGAAGCGCAGCTAAACTATCGTTCATTTTCTATCACAATAGGGTCCTATCAAACTCAAAATTATACAAGAACTGTGACAAATGTTGGCCCGGCTTATTCAACCTACAAATCGGAGATTTTGAAACCCGAGAAATGGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

592

Amino Acids

63.33

Weight (kDa)

6.71

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 110 - 499 3.8e-33 Subtilase family
PA PF02225 286 - 370 1e-11 PA domain
fn3_6 PF17766 548 - 589 6.6e-06 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1462
Acc36I ACCTGC 3 cut(s) 494, 1091, 1462
Acc65I GGTACC 1 cut(s) 1382
AccB1I GGYRCC 2 cut(s) 416, 1382
AclWI GGATC 1 cut(s) 919
AcoI YGGCCR 2 cut(s) 24, 477
AcsI RAATTY 5 cut(s) 316, 654, 679, 831, 1242
AcuI CTGAAG 1 cut(s) 1656
AcyI GRCGYC 1 cut(s) 417
AfaI GTAC 4 cut(s) 280, 1071, 1384, 1554
AfiI CCNNNNNNNGG 1 cut(s) 1767
AgsI TTSAA 8 cut(s) 268, 274, 823, 929, 1097, 1249, 1734, 1756
AhlI ACTAGT 1 cut(s) 128
AjnI CCWGG 3 cut(s) 147, 805, 1237
AleI CACNNNNGTG 1 cut(s) 1407
Alw21I GWGCWC 1 cut(s) 570
Alw26I GTCTC 1 cut(s) 352
AlwI GGATC 1 cut(s) 919
AlwNI CAGNNNCTG 1 cut(s) 452
Ama87I CYCGRG 1 cut(s) 1760
AoxI GGCC 8 cut(s) 24, 367, 477, 685, 714, 1292, 1568, 1720
ApeKI GCWGC 9 cut(s) 38, 154, 395, 530, 1010, 1286, 1418, 1457, 1642
ApoI RAATTY 5 cut(s) 316, 654, 679, 831, 1242
Asp718I GGTACC 1 cut(s) 1382
AspLEI GCGC 2 cut(s) 419, 1642
AspS9I GGNCC 4 cut(s) 715, 1220, 1677, 1721
AsuC2I CCSGG 1 cut(s) 1724
AsuHPI GGTGA 8 cut(s) 206, 253, 371, 721, 821, 837, 1443, 1585
AsuII TTCGAA 1 cut(s) 646
AsuNHI GCTAGC 2 cut(s) 526, 1232
AvaI CYCGRG 1 cut(s) 1760
AvaII GGWCC 2 cut(s) 1220, 1677
AxyI CCTNAGG 1 cut(s) 1565
BaeI ACNNNNGTAYC 2 cut(s) 1374, 1407
BalI TGGCCA 2 cut(s) 26, 479
BanI GGYRCC 2 cut(s) 416, 1382
Bbv12I GWGCWC 1 cut(s) 570
BbvI GCAGC 9 cut(s) 50, 166, 382, 542, 997, 1273, 1430, 1444, 1654
BccI CCATC 4 cut(s) 77, 488, 608, 615
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 3 cut(s) 149, 807, 1239
BclI TGATCA 1 cut(s) 1590
BcnI CCSGG 1 cut(s) 1724
BcoDI GTCTC 1 cut(s) 352
BcuI ACTAGT 1 cut(s) 128
BfaI CTAG 7 cut(s) 53, 129, 387, 527, 869, 1022, 1233
BfmI CTRYAG 2 cut(s) 694, 1143
BfoI RGCGCY 1 cut(s) 420
BfuAI ACCTGC 3 cut(s) 494, 1091, 1462
BisI GCNGC 9 cut(s) 39, 155, 396, 531, 1011, 1287, 1419, 1458, 1643
BlsI GCNGC 9 cut(s) 40, 156, 397, 532, 1012, 1288, 1420, 1459, 1644
Bme1390I CCNGG 4 cut(s) 149, 807, 1239, 1724
Bme18I GGWCC 2 cut(s) 1220, 1677
BmeT110I CYCGRG 1 cut(s) 1760
BmgT120I GGNCC 4 cut(s) 715, 1220, 1677, 1721
BmiI GGNNCC 5 cut(s) 418, 600, 717, 1384, 1678
BmrFI CCNGG 4 cut(s) 149, 807, 1239, 1724
BmsI GCATC 2 cut(s) 458, 1051
BmtI GCTAGC 2 cut(s) 530, 1236
Bpu10I CCTNAGC 1 cut(s) 1054
Bpu14I TTCGAA 1 cut(s) 646
BpuMI CCSGG 1 cut(s) 1724
BsaAI YACGTR 1 cut(s) 1552
BsaHI GRCGYC 1 cut(s) 417
BsaJI CCNNGG 9 cut(s) 148, 322, 370, 498, 594, 719, 805, 906, 1237
BsaWI WCCGGW 1 cut(s) 238
Bsc4I CCNNNNNNNGG 1 cut(s) 1767
Bse1I ACTGG 2 cut(s) 34, 1451
Bse21I CCTNAGG 1 cut(s) 1565
Bse3DI GCAATG 5 cut(s) 25, 75, 456, 848, 1413
BseBI CCWGG 3 cut(s) 149, 807, 1239
BseDI CCNNGG 9 cut(s) 148, 322, 370, 498, 594, 719, 805, 906, 1237
BseGI GGATG 3 cut(s) 100, 202, 568
BseLI CCNNNNNNNGG 1 cut(s) 1767
BseMI GCAATG 5 cut(s) 25, 75, 456, 848, 1413
BseMII CTCAG 4 cut(s) 885, 1401, 1579, 1608
BseNI ACTGG 2 cut(s) 34, 1451
BseRI GAGGAG 2 cut(s) 225, 975
BseXI GCAGC 9 cut(s) 50, 166, 382, 542, 997, 1273, 1430, 1444, 1654
BseYI CCCAGC 1 cut(s) 1537
BsgI GTGCAG 1 cut(s) 478
BshFI GGCC 8 cut(s) 26, 369, 479, 687, 716, 1294, 1570, 1722
BshNI GGYRCC 2 cut(s) 416, 1382
BsiHKAI GWGCWC 1 cut(s) 570
BsiHKCI CYCGRG 1 cut(s) 1760
BsiSI CCGG 2 cut(s) 239, 1724
BsiWI CGTACG 1 cut(s) 278
BslI CCNNNNNNNGG 1 cut(s) 1767
BsmAI GTCTC 1 cut(s) 352
BsnI GGCC 8 cut(s) 26, 369, 479, 687, 716, 1294, 1570, 1722
BsoBI CYCGRG 1 cut(s) 1760
Bsp119I TTCGAA 1 cut(s) 646
Bsp1286I GDGCHC 1 cut(s) 570
Bsp143I GATC 4 cut(s) 199, 911, 1526, 1590
Bsp19I CCATGG 1 cut(s) 370
BspANI GGCC 8 cut(s) 26, 369, 479, 687, 716, 1294, 1570, 1722
BspCNI CTCAG 4 cut(s) 886, 1400, 1578, 1609
BspLI GGNNCC 5 cut(s) 418, 600, 717, 1384, 1678
BspMI ACCTGC 3 cut(s) 494, 1091, 1462
BspOI GCTAGC 2 cut(s) 530, 1236
BspPI GGATC 1 cut(s) 919
BspT104I TTCGAA 1 cut(s) 646
BspT107I GGYRCC 2 cut(s) 416, 1382
BsrDI GCAATG 5 cut(s) 25, 75, 456, 848, 1413
BsrI ACTGG 2 cut(s) 34, 1451
BssECI CCNNGG 9 cut(s) 148, 322, 370, 498, 594, 719, 805, 906, 1237
BssMI GATC 4 cut(s) 199, 911, 1526, 1590
BssNI GRCGYC 1 cut(s) 417
BssT1I CCWWGG 4 cut(s) 322, 370, 719, 906
Bst2UI CCWGG 3 cut(s) 149, 807, 1239
Bst4CI ACNGT 6 cut(s) 286, 557, 576, 733, 1153, 1708
BstACI GRCGYC 1 cut(s) 417
BstAPI GCANNNNNTGC 3 cut(s) 467, 509, 1007
BstBAI YACGTR 1 cut(s) 1552
BstBI TTCGAA 1 cut(s) 646
BstC8I GCNNGC 5 cut(s) 96, 421, 528, 742, 1234
BstDEI CTNAG 6 cut(s) 894, 1054, 1387, 1406, 1565, 1617
BstDSI CCRYGG 1 cut(s) 370
BstEII GGTNACC 1 cut(s) 1449
BstF5I GGATG 3 cut(s) 100, 202, 568
BstH2I RGCGCY 1 cut(s) 420
BstHHI GCGC 2 cut(s) 419, 1642
BstKTI GATC 4 cut(s) 202, 914, 1529, 1593
BstMAI GTCTC 1 cut(s) 352
BstMBI GATC 4 cut(s) 199, 911, 1526, 1590
BstMWI GCNNNNNNNGC 5 cut(s) 467, 476, 509, 1007, 1233
BstNI CCWGG 3 cut(s) 149, 807, 1239
BstNSI RCATGY 1 cut(s) 98
BstPI GGTNACC 1 cut(s) 1449
BstSCI CCNGG 4 cut(s) 147, 805, 1237, 1722
BstSFI CTRYAG 2 cut(s) 694, 1143
BstSNI TACGTA 1 cut(s) 1552
BstV1I GCAGC 9 cut(s) 50, 166, 382, 542, 997, 1273, 1430, 1444, 1654
BstX2I RGATCY 1 cut(s) 911
BstXI CCANNNNNNTGG 1 cut(s) 614
BstYI RGATCY 1 cut(s) 911
Bsu36I CCTNAGG 1 cut(s) 1565
BsuRI GGCC 8 cut(s) 26, 369, 479, 687, 716, 1294, 1570, 1722
BtgI CCRYGG 1 cut(s) 370
BtsCI GGATG 3 cut(s) 100, 202, 568
BtsI GCAGTG 2 cut(s) 540, 1558
BtsIMutI CAGTG 9 cut(s) 27, 291, 540, 572, 634, 1035, 1167, 1395, 1558
BveI ACCTGC 3 cut(s) 494, 1091, 1462
Cac8I GCNNGC 5 cut(s) 96, 421, 528, 742, 1234
CaiI CAGNNNCTG 1 cut(s) 452
CfoI GCGC 2 cut(s) 419, 1642
Cfr13I GGNCC 4 cut(s) 715, 1220, 1677, 1721
Csp6I GTAC 4 cut(s) 279, 1070, 1383, 1553
CspCI CAANNNNNGTGG 2 cut(s) 16, 51
CviAII CATG 8 cut(s) 95, 371, 703, 1015, 1067, 1268, 1290, 1395
CviQI GTAC 4 cut(s) 279, 1070, 1383, 1553
DdeI CTNAG 6 cut(s) 894, 1054, 1387, 1406, 1565, 1617
DinI GGCGCC 1 cut(s) 418
DpnI GATC 4 cut(s) 201, 913, 1528, 1592
DpnII GATC 4 cut(s) 199, 911, 1526, 1590
EaeI YGGCCR 2 cut(s) 24, 477
Eco105I TACGTA 1 cut(s) 1552
Eco130I CCWWGG 4 cut(s) 322, 370, 719, 906
Eco47I GGWCC 2 cut(s) 1220, 1677
Eco57I CTGAAG 1 cut(s) 1656
Eco81I CCTNAGG 1 cut(s) 1565
Eco88I CYCGRG 1 cut(s) 1760
Eco91I GGTNACC 1 cut(s) 1449
EcoO109I RGGNCCY 2 cut(s) 715, 1677
EcoO65I GGTNACC 1 cut(s) 1449
EcoRII CCWGG 3 cut(s) 147, 805, 1237
EcoT14I CCWWGG 4 cut(s) 322, 370, 719, 906
EgeI GGCGCC 1 cut(s) 418
EheI GGCGCC 1 cut(s) 418
ErhI CCWWGG 4 cut(s) 322, 370, 719, 906
FaeI CATG 8 cut(s) 98, 374, 706, 1018, 1070, 1271, 1293, 1398
FalI AAGNNNNNCTT 2 cut(s) 574, 606
FatI CATG 8 cut(s) 94, 370, 702, 1014, 1066, 1267, 1289, 1394
FbaI TGATCA 1 cut(s) 1590
Fnu4HI GCNGC 9 cut(s) 39, 155, 396, 531, 1011, 1287, 1419, 1458, 1643
FokI GGATG 3 cut(s) 87, 189, 575
Fsp4HI GCNGC 9 cut(s) 39, 155, 396, 531, 1011, 1287, 1419, 1458, 1643
FspBI CTAG 7 cut(s) 53, 129, 387, 527, 869, 1022, 1233
GlaI GCGC 2 cut(s) 418, 1641
GluI GCNGC 9 cut(s) 39, 155, 396, 531, 1011, 1287, 1419, 1458, 1643
GsaI CCCAGC 1 cut(s) 1541
HaeII RGCGCY 1 cut(s) 420
HaeIII GGCC 8 cut(s) 26, 369, 479, 687, 716, 1294, 1570, 1722
HapII CCGG 2 cut(s) 239, 1724
HhaI GCGC 2 cut(s) 419, 1642
Hin1I GRCGYC 1 cut(s) 417
Hin1II CATG 8 cut(s) 98, 374, 706, 1018, 1070, 1271, 1293, 1398
Hin6I GCGC 2 cut(s) 417, 1640
HinP1I GCGC 2 cut(s) 417, 1640
HindIII AAGCTT 1 cut(s) 299
HinfI GANTC 5 cut(s) 724, 785, 812, 1018, 1626
HpaII CCGG 2 cut(s) 239, 1724
HphI GGTGA 8 cut(s) 206, 253, 371, 721, 821, 837, 1443, 1585
Hpy166II GTNNAC 4 cut(s) 7, 1027, 1436, 1609
Hpy188I TCNGA 4 cut(s) 170, 1362, 1618, 1747
Hpy188III TCNNGA 6 cut(s) 104, 536, 611, 1215, 1429, 1502
Hpy8I GTNNAC 4 cut(s) 7, 1027, 1436, 1609
HpyAV CCTTC 3 cut(s) 359, 923, 1499
HpyCH4III ACNGT 6 cut(s) 286, 557, 576, 733, 1153, 1708
HpyCH4IV ACGT 1 cut(s) 1551
HpyF10VI GCNNNNNNNGC 5 cut(s) 467, 476, 509, 1007, 1233
HpyF3I CTNAG 6 cut(s) 894, 1054, 1387, 1406, 1565, 1617
HpySE526I ACGT 1 cut(s) 1551
Hsp92I GRCGYC 1 cut(s) 417
Hsp92II CATG 8 cut(s) 98, 374, 706, 1018, 1070, 1271, 1293, 1398
HspAI GCGC 2 cut(s) 417, 1640
KasI GGCGCC 1 cut(s) 416
KpnI GGTACC 1 cut(s) 1386
Ksp22I TGATCA 1 cut(s) 1590
Kzo9I GATC 4 cut(s) 199, 911, 1526, 1590
LmnI GCTCC 1 cut(s) 737
Lsp1109I GCAGC 9 cut(s) 50, 166, 382, 542, 997, 1273, 1430, 1444, 1654
LweI GCATC 2 cut(s) 458, 1051
MaeI CTAG 7 cut(s) 53, 129, 387, 527, 869, 1022, 1233
MaeII ACGT 1 cut(s) 1551
MaeIII GTNAC 4 cut(s) 1312, 1449, 1627, 1708
MalI GATC 4 cut(s) 201, 913, 1528, 1592
MboI GATC 4 cut(s) 199, 911, 1526, 1590
MboII GAAGA 4 cut(s) 209, 374, 981, 998
MfeI CAATTG 1 cut(s) 1515
MflI RGATCY 1 cut(s) 911
MhlI GDGCHC 1 cut(s) 570
MlsI TGGCCA 2 cut(s) 26, 479
MluCI AATT 9 cut(s) 187, 316, 642, 654, 679, 831, 1242, 1515, 1693
MluNI TGGCCA 2 cut(s) 26, 479
Mly113I GGCGCC 1 cut(s) 417
MlyI GAGTC 1 cut(s) 1635
MmeI TCCRAC 1 cut(s) 715
Mox20I TGGCCA 2 cut(s) 26, 479
MscI TGGCCA 2 cut(s) 26, 479
MseI TTAA 3 cut(s) 1275, 1424, 1464
MslI CAYNNNNRTG 2 cut(s) 612, 1407
Msp20I TGGCCA 2 cut(s) 26, 479
MspA1I CMGCKG 3 cut(s) 666, 674, 1634
MspI CCGG 2 cut(s) 239, 1724
MspR9I CCNGG 4 cut(s) 149, 807, 1239, 1724
MunI CAATTG 1 cut(s) 1515
MvaI CCWGG 3 cut(s) 149, 807, 1239
MwoI GCNNNNNNNGC 5 cut(s) 467, 476, 509, 1007, 1233
NarI GGCGCC 1 cut(s) 417
NciI CCSGG 1 cut(s) 1724
NcoI CCATGG 1 cut(s) 370
NdeII GATC 4 cut(s) 199, 911, 1526, 1590
NheI GCTAGC 2 cut(s) 526, 1232
NlaIII CATG 8 cut(s) 98, 374, 706, 1018, 1070, 1271, 1293, 1398
NlaIV GGNNCC 5 cut(s) 418, 600, 717, 1384, 1678
NmuCI GTSAC 4 cut(s) 1312, 1449, 1627, 1708
NspI RCATGY 1 cut(s) 98
NspV TTCGAA 1 cut(s) 646
OliI CACNNNNGTG 1 cut(s) 1407
PaeI GCATGC 1 cut(s) 98
PaqCI CACCTGC 1 cut(s) 1462
PfeI GAWTC 4 cut(s) 724, 785, 812, 1018
Pfl23II CGTACG 1 cut(s) 278
PkrI GCNGC 9 cut(s) 40, 156, 397, 532, 1012, 1288, 1420, 1459, 1644
PleI GAGTC 1 cut(s) 1634
PluTI GGCGCC 1 cut(s) 420
PpsI GAGTC 1 cut(s) 1634
Ppu21I YACGTR 1 cut(s) 1552
PpuMI RGGWCCY 1 cut(s) 1677
Psp5II RGGWCCY 1 cut(s) 1677
Psp6I CCWGG 3 cut(s) 147, 805, 1237
PspEI GGTNACC 1 cut(s) 1449
PspFI CCCAGC 1 cut(s) 1537
PspGI CCWGG 3 cut(s) 147, 805, 1237
PspLI CGTACG 1 cut(s) 278
PspN4I GGNNCC 5 cut(s) 418, 600, 717, 1384, 1678
PspPI GGNCC 4 cut(s) 715, 1220, 1677, 1721
PspPPI RGGWCCY 1 cut(s) 1677
PstNI CAGNNNCTG 1 cut(s) 452
PsuI RGATCY 1 cut(s) 911
PvuII CAGCTG 3 cut(s) 666, 674, 1634
RsaI GTAC 4 cut(s) 280, 1071, 1384, 1554
RsaNI GTAC 4 cut(s) 279, 1070, 1383, 1553
RseI CAYNNNNRTG 2 cut(s) 612, 1407
SaqAI TTAA 3 cut(s) 1275, 1424, 1464
SatI GCNGC 9 cut(s) 39, 155, 396, 531, 1011, 1287, 1419, 1458, 1643
Sau3AI GATC 4 cut(s) 199, 911, 1526, 1590
Sau96I GGNCC 4 cut(s) 715, 1220, 1677, 1721
SchI GAGTC 1 cut(s) 1635
ScrFI CCNGG 4 cut(s) 149, 807, 1239, 1724
SduI GDGCHC 1 cut(s) 570
SfaNI GCATC 2 cut(s) 458, 1051
SfcI CTRYAG 2 cut(s) 694, 1143
SfoI GGCGCC 1 cut(s) 418
SfuI TTCGAA 1 cut(s) 646
SinI GGWCC 2 cut(s) 1220, 1677
SmiMI CAYNNNNRTG 2 cut(s) 612, 1407
SnaBI TACGTA 1 cut(s) 1552
SpeI ACTAGT 1 cut(s) 128
SphI GCATGC 1 cut(s) 98
Sse9I AATT 9 cut(s) 187, 316, 642, 654, 679, 831, 1242, 1515, 1693
SspDI GGCGCC 1 cut(s) 416
SspI AATATT 1 cut(s) 797
SspMI CTAG 7 cut(s) 53, 129, 387, 527, 869, 1022, 1233
StyD4I CCNGG 4 cut(s) 147, 805, 1237, 1722
StyI CCWWGG 4 cut(s) 322, 370, 719, 906
TaaI ACNGT 6 cut(s) 286, 557, 576, 733, 1153, 1708
TaiI ACGT 1 cut(s) 1554
TaqI TCGA 2 cut(s) 646, 1503
TaqII GACCGA 1 cut(s) 1237
TasI AATT 9 cut(s) 187, 316, 642, 654, 679, 831, 1242, 1515, 1693
TatI WGTACW 1 cut(s) 1069
TfiI GAWTC 4 cut(s) 724, 785, 812, 1018
Tru1I TTAA 3 cut(s) 1275, 1424, 1464
Tru9I TTAA 3 cut(s) 1275, 1424, 1464
TscAI CASTG 9 cut(s) 34, 291, 547, 579, 634, 1042, 1174, 1395, 1565
TseFI GTSAC 4 cut(s) 1312, 1449, 1627, 1708
TseI GCWGC 9 cut(s) 38, 154, 395, 530, 1010, 1286, 1418, 1457, 1642
Tsp45I GTSAC 4 cut(s) 1312, 1449, 1627, 1708
TspDTI ATGAA 2 cut(s) 234, 1647
TspRI CASTG 9 cut(s) 34, 291, 547, 579, 634, 1042, 1174, 1395, 1565
VpaK11BI GGWCC 2 cut(s) 1220, 1677
XapI RAATTY 5 cut(s) 316, 654, 679, 831, 1242
XceI RCATGY 1 cut(s) 98
XspI CTAG 7 cut(s) 53, 129, 387, 527, 869, 1022, 1233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.