FvH4_7g18690

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005197
Physical Location & Seq
Forward (+)
15525081 .. 15528928
3848 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g18690.t7

Sequence Viewer

Length: 1881 bp
ATGAAACGCTGGAAAGATGAGGAAGACCCCGCTGAACCAAGGTGCTGTATGAATATATCACAAAGGATACCAAGCCTGCTCGGATGCATATTTTCATGGCTTGGAACATGTCTCTTCAAAGTATTCCGGAAAATTCTTGACAATCTTGCGGATTTAAAGTGGAAGGTCGGATGCTTCTTCCGGGAGAAAAATAGACTTGTAATAAGACCATGGTGGGATATAGTACTTGTAATATCTTGTGTCATTGCCGTCTCACTGGATCCTTTATTCTTTTACATTCCGACCATCAATGAGGAAGAAAAGTGCCTTGAAATGGACACCAAGTTGAGGACTGTATCTCTCGTTTTGCGATCATTCACGGACATCATCTTCATTTTAGACATTTTAGAGCAAATTTCTAAGGCTTCAACAATAGCTGAGATATGGTGGTCACTTGCAACAGACTTTCTTTGTATACTTCCCGCTCCACAAGTGTTGATCGTAGTCATCTTTTACAAAATCGATTTTGGATATTTGAATAATTCAACGATTATGAATGCTCTTCTTCTCATTCAATATGCACCAAGGATTTATCAACTTAATCAATCGTTTGACAATCTTGAGCATGGTATCGGATTATGGGTCAAAATTGTATTCAATTTTTTCCAATACATCCTTGCTTGCCACATACTTGGAGCCTCGTGGTTTTTCTTTTCTATTCAACGAGAGATGTTATGTTGGTACCAAGTTTGTGTAAAGCATAAGACTTGCGGAACATATTTCAAGTGCAACGACGTCAATAGTTCGAGAAATATCACAAAACTAGTTGAACTTTGTCCAATGAATCCACCAAATTCCACCGTATATGATTTCGGGATATTCGTTAATCCTCTGGGAACTAGTAGCAGAACACCAATAAACTTCTCGACGAAGTTCTCATACTCCTTTTGGTGGGGAGTGCGAAATTTAAGTAATTTTGGCACAAATCTTCAAACAAGTACGTATCTGTGGGAAAATTGGTTTGCAAGTCTGATTTCCATCATCGGCTTGCTACTCTTTTTATATCTCATTGGAAATAATGTTCAGACATATATGCAAATGAAAACTAAGAAAGCGGAGGAGGAAGCTGAAAGGTTACGGCTTGAAGCTGAAAGGATAAGGCGTCAACAGACAAGGATAAATTTATGGATGGAACGACATGATTTCCCTTCGTTTGCAAGGAAAAACATCGAGGAAAAAATGACAGAGAAGTATAAAGAAAACAAAGAGGCTAATCTTGATAATGTACCGACTCTATTATCCTTTATAACTTCAGGTTACAAGAGGTACGCAAGGGTTAAAATCTGCCAGAAAATGCTAGAGAATGTGCCATTGCTTAAGGATATGGATTTAGATGTGTTGCTAACAATTAGTAGAAGAATGGAGATAGTGGTGTACCCGGAAAATACTTGGATTGTTGAAATGGACGAGCCATTGGATCGCATGGTTTTCATTACGGAAGGGACTATGGGGATCTACACGGCTAGATCAGACTGTTCTCCTTCAGCAGTCATTCAAGGGTCTCTTGAGAAATGCAGTATTTATGGACATGAACGACTACTGAGGTGGGTACTCTCACCCAACCCAGATCTCGACCGCCTTCCTATCTCGAAAGAAGCTGTCAAGTGTAGTACCAAAGTAGAAGGCTTTGTTCTCACAGCCGATGACTTGAAGACAGTGCTATCTGATAAAGGTGTAATCGGGGAGAATTGTTCTGATGAAGATGATGCGGAAGTTAGTGATTCTAGTCTGAGATCGTCATTCCTTGTAGCAACAGCAGTGTCTCGTTTCCGGCGCCGCCTTCGAAGAAAGAAGCCTGCATTACATTTAGATTTCGACCCCTCTAGCTTCAAAGGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

627

Amino Acids

72.71

Weight (kDa)

8.14

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 7 - 259 3.1e-09 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1286
AatII GACGTC 1 cut(s) 777
Acc65I GGTACC 1 cut(s) 720
AccB1I GGYRCC 2 cut(s) 720, 1812
AccBSI CCGCTC 1 cut(s) 464
AccI GTMKAC 1 cut(s) 454
AccIII TCCGGA 1 cut(s) 126
AciI CCGC 8 cut(s) 30, 149, 462, 750, 1094, 1615, 1748, 1816
AclWI GGATC 4 cut(s) 254, 267, 1464, 1499
AcsI RAATTY 5 cut(s) 132, 393, 832, 943, 1159
AcuI CTGAAG 2 cut(s) 1275, 1506
AcyI GRCGYC 3 cut(s) 774, 1141, 1813
AfaI GTAC 8 cut(s) 225, 722, 979, 1266, 1307, 1415, 1590, 1651
AfiI CCNNNNNNNGG 3 cut(s) 313, 327, 930
AflII CTTAAG 1 cut(s) 1355
AflIII ACRYGT 1 cut(s) 107
AhlI ACTAGT 2 cut(s) 802, 878
AloI GAACNNNNNNTCC 2 cut(s) 1044, 1076
AluBI AGCT 5 cut(s) 416, 1106, 1127, 1637, 1866
AluI AGCT 5 cut(s) 416, 1106, 1127, 1637, 1866
Alw26I GTCTC 4 cut(s) 116, 256, 1545, 1806
AlwI GGATC 4 cut(s) 254, 267, 1464, 1499
Aor13HI TCCGGA 1 cut(s) 126
ApoI RAATTY 5 cut(s) 132, 393, 832, 943, 1159
Asp718I GGTACC 1 cut(s) 720
AspLEI GCGC 1 cut(s) 1815
AsuC2I CCSGG 2 cut(s) 182, 1418
AsuHPI GGTGA 1 cut(s) 1587
AsuII TTCGAA 1 cut(s) 1822
BamHI GGATCC 1 cut(s) 259
BanI GGYRCC 2 cut(s) 720, 1812
BarI GAAGNNNNNNTAC 2 cut(s) 902, 934
BauI CACGAG 1 cut(s) 679
BbsI GAAGAC 2 cut(s) 30, 1697
BccI CCATC 3 cut(s) 293, 1025, 1162
BceAI ACGGC 3 cut(s) 233, 1133, 1515
BciVI GTATCC 1 cut(s) 60
BcnI CCSGG 2 cut(s) 182, 1418
BcoDI GTCTC 4 cut(s) 116, 256, 1545, 1806
BcuI ACTAGT 2 cut(s) 802, 878
BfaI CTAG 6 cut(s) 803, 879, 1337, 1503, 1764, 1863
BfoI RGCGCY 1 cut(s) 1816
BfrI CTTAAG 1 cut(s) 1355
BfuI GTATCC 1 cut(s) 60
BglII AGATCT 1 cut(s) 1606
BisI GCNGC 1 cut(s) 1816
BlsI GCNGC 1 cut(s) 1817
BmcAI AGTACT 1 cut(s) 225
Bme1390I CCNGG 2 cut(s) 182, 1418
BmiI GGNNCC 4 cut(s) 261, 676, 722, 1814
BmrFI CCNGG 2 cut(s) 182, 1418
BmsI GCATC 3 cut(s) 74, 161, 1735
BpiI GAAGAC 2 cut(s) 30, 1697
Bpu14I TTCGAA 1 cut(s) 1822
BpuEI CTTGAG 2 cut(s) 620, 1565
BpuMI CCSGG 2 cut(s) 182, 1418
Bsa29I ATCGAT 1 cut(s) 501
BsaAI YACGTR 1 cut(s) 981
BsaBI GATNNNNATC 1 cut(s) 1016
BsaHI GRCGYC 3 cut(s) 774, 1141, 1813
BsaI GGTCTC 1 cut(s) 1545
BsaJI CCNNGG 3 cut(s) 38, 209, 563
BsaWI WCCGGW 1 cut(s) 126
Bsc4I CCNNNNNNNGG 3 cut(s) 313, 327, 930
Bse1I ACTGG 1 cut(s) 261
Bse3DI GCAATG 2 cut(s) 243, 1349
Bse8I GATNNNNATC 1 cut(s) 1016
BseAI TCCGGA 1 cut(s) 126
BseCI ATCGAT 1 cut(s) 501
BseDI CCNNGG 3 cut(s) 38, 209, 563
BseGI GGATG 4 cut(s) 89, 176, 651, 1173
BseJI GATNNNNATC 1 cut(s) 1016
BseLI CCNNNNNNNGG 3 cut(s) 313, 327, 930
BseMI GCAATG 2 cut(s) 243, 1349
BseMII CTCAG 3 cut(s) 408, 1571, 1760
BseNI ACTGG 1 cut(s) 261
BseRI GAGGAG 1 cut(s) 1112
Bsh1285I CGRYCG 1 cut(s) 1615
BshNI GGYRCC 2 cut(s) 720, 1812
BshVI ATCGAT 1 cut(s) 501
BsiEI CGRYCG 1 cut(s) 1615
BsiSI CCGG 4 cut(s) 127, 181, 1418, 1810
BslFI GGGAC 1 cut(s) 1495
BslI CCNNNNNNNGG 3 cut(s) 313, 327, 930
BsmAI GTCTC 4 cut(s) 116, 256, 1545, 1806
BsmBI CGTCTC 1 cut(s) 256
BsmFI GGGAC 1 cut(s) 1495
BsmI GAATGC 1 cut(s) 541
Bso31I GGTCTC 1 cut(s) 1545
Bsp119I TTCGAA 1 cut(s) 1822
Bsp13I TCCGGA 1 cut(s) 126
Bsp143I GATC 8 cut(s) 259, 350, 477, 1456, 1491, 1505, 1606, 1772
Bsp19I CCATGG 1 cut(s) 209
BspACI CCGC 8 cut(s) 30, 149, 462, 750, 1094, 1615, 1748, 1816
BspCNI CTCAG 3 cut(s) 409, 1572, 1761
BspDI ATCGAT 1 cut(s) 501
BspEI TCCGGA 1 cut(s) 126
BspLI GGNNCC 4 cut(s) 261, 676, 722, 1814
BspPI GGATC 4 cut(s) 254, 267, 1464, 1499
BspQI GCTCTTC 1 cut(s) 546
BspT104I TTCGAA 1 cut(s) 1822
BspT107I GGYRCC 2 cut(s) 720, 1812
BspTI CTTAAG 1 cut(s) 1355
BspTNI GGTCTC 1 cut(s) 1545
BsrBI CCGCTC 1 cut(s) 464
BsrDI GCAATG 2 cut(s) 243, 1349
BsrI ACTGG 1 cut(s) 261
BssECI CCNNGG 3 cut(s) 38, 209, 563
BssMI GATC 8 cut(s) 259, 350, 477, 1456, 1491, 1505, 1606, 1772
BssNAI GTATAC 1 cut(s) 455
BssNI GRCGYC 3 cut(s) 774, 1141, 1813
BssSI CACGAG 1 cut(s) 679
BssT1I CCWWGG 3 cut(s) 38, 209, 563
Bst1107I GTATAC 1 cut(s) 455
Bst2BI CACGAG 1 cut(s) 679
Bst4CI ACNGT 4 cut(s) 334, 841, 1514, 1696
Bst6I CTCTTC 2 cut(s) 119, 546
BstACI GRCGYC 3 cut(s) 774, 1141, 1813
BstAFI CTTAAG 1 cut(s) 1355
BstBAI YACGTR 1 cut(s) 981
BstBI TTCGAA 1 cut(s) 1822
BstC8I GCNNGC 4 cut(s) 77, 661, 1028, 1836
BstDEI CTNAG 5 cut(s) 399, 417, 1086, 1580, 1769
BstDSI CCRYGG 1 cut(s) 209
BstF5I GGATG 4 cut(s) 89, 176, 651, 1173
BstH2I RGCGCY 1 cut(s) 1816
BstHHI GCGC 1 cut(s) 1815
BstKTI GATC 8 cut(s) 262, 353, 480, 1459, 1494, 1508, 1609, 1775
BstMAI GTCTC 4 cut(s) 116, 256, 1545, 1806
BstMBI GATC 8 cut(s) 259, 350, 477, 1456, 1491, 1505, 1606, 1772
BstMCI CGRYCG 1 cut(s) 1615
BstNSI RCATGY 1 cut(s) 111
BstSCI CCNGG 2 cut(s) 180, 1416
BstSNI TACGTA 1 cut(s) 981
BstV2I GAAGAC 2 cut(s) 30, 1697
BstX2I RGATCY 3 cut(s) 259, 1491, 1606
BstXI CCANNNNNNTGG 1 cut(s) 671
BstYI RGATCY 3 cut(s) 259, 1491, 1606
BstZ17I GTATAC 1 cut(s) 455
Bsu15I ATCGAT 1 cut(s) 501
BsuI GTATCC 1 cut(s) 60
BsuTUI ATCGAT 1 cut(s) 501
BtgI CCRYGG 1 cut(s) 209
BtsCI GGATG 4 cut(s) 89, 176, 651, 1173
BtsI GCAGTG 1 cut(s) 1803
BtsIMutI CAGTG 3 cut(s) 254, 1701, 1803
Cac8I GCNNGC 4 cut(s) 77, 661, 1028, 1836
CfoI GCGC 1 cut(s) 1815
ClaI ATCGAT 1 cut(s) 501
CseI GACGC 1 cut(s) 1130
Csp6I GTAC 8 cut(s) 224, 721, 978, 1265, 1306, 1414, 1589, 1650
CspCI CAANNNNNGTGG 2 cut(s) 456, 491
CviAII CATG 7 cut(s) 96, 108, 210, 605, 1178, 1462, 1568
CviQI GTAC 8 cut(s) 224, 721, 978, 1265, 1306, 1414, 1589, 1650
DdeI CTNAG 5 cut(s) 399, 417, 1086, 1580, 1769
DinI GGCGCC 1 cut(s) 1814
DpnI GATC 8 cut(s) 261, 352, 479, 1458, 1493, 1507, 1608, 1774
DpnII GATC 8 cut(s) 259, 350, 477, 1456, 1491, 1505, 1606, 1772
DraI TTTAAA 1 cut(s) 156
Eam1104I CTCTTC 2 cut(s) 119, 546
EarI CTCTTC 2 cut(s) 119, 546
Eco105I TACGTA 1 cut(s) 981
Eco130I CCWWGG 3 cut(s) 38, 209, 563
Eco31I GGTCTC 1 cut(s) 1545
Eco57I CTGAAG 2 cut(s) 1275, 1506
EcoT14I CCWWGG 3 cut(s) 38, 209, 563
EcoT22I ATGCAT 1 cut(s) 89
EgeI GGCGCC 1 cut(s) 1814
EheI GGCGCC 1 cut(s) 1814
ErhI CCWWGG 3 cut(s) 38, 209, 563
Esp3I CGTCTC 1 cut(s) 256
FaeI CATG 7 cut(s) 99, 111, 213, 608, 1181, 1465, 1571
FalI AAGNNNNNCTT 2 cut(s) 1527, 1559
FaqI GGGAC 1 cut(s) 1495
FatI CATG 7 cut(s) 95, 107, 209, 604, 1177, 1461, 1567
FauI CCCGC 2 cut(s) 37, 469
FblI GTMKAC 1 cut(s) 454
Fnu4HI GCNGC 1 cut(s) 1816
FokI GGATG 4 cut(s) 96, 183, 638, 1180
Fsp4HI GCNGC 1 cut(s) 1816
FspBI CTAG 6 cut(s) 803, 879, 1337, 1503, 1764, 1863
GlaI GCGC 1 cut(s) 1814
GluI GCNGC 1 cut(s) 1816
HaeII RGCGCY 1 cut(s) 1816
HapII CCGG 4 cut(s) 127, 181, 1418, 1810
HgaI GACGC 1 cut(s) 1130
HhaI GCGC 1 cut(s) 1815
Hin1I GRCGYC 3 cut(s) 774, 1141, 1813
Hin1II CATG 7 cut(s) 99, 111, 213, 608, 1181, 1465, 1571
Hin6I GCGC 1 cut(s) 1813
HinP1I GCGC 1 cut(s) 1813
HincII GTYRAC 1 cut(s) 1145
HindII GTYRAC 1 cut(s) 1145
HinfI GANTC 3 cut(s) 823, 1270, 1760
HpaII CCGG 4 cut(s) 127, 181, 1418, 1810
HphI GGTGA 1 cut(s) 1587
Hpy166II GTNNAC 3 cut(s) 455, 1145, 1414
Hpy8I GTNNAC 3 cut(s) 455, 1145, 1414
Hpy99I CGWCG 2 cut(s) 776, 910
HpyAV CCTTC 7 cut(s) 157, 1197, 1472, 1530, 1628, 1655, 1829
HpyCH4III ACNGT 4 cut(s) 334, 841, 1514, 1696
HpyCH4IV ACGT 2 cut(s) 774, 980
HpyCH4V TGCA 9 cut(s) 87, 437, 560, 768, 1004, 1075, 1196, 1554, 1838
HpyF3I CTNAG 5 cut(s) 399, 417, 1086, 1580, 1769
HpySE526I ACGT 2 cut(s) 774, 980
Hsp92I GRCGYC 3 cut(s) 774, 1141, 1813
Hsp92II CATG 7 cut(s) 99, 111, 213, 608, 1181, 1465, 1571
HspAI GCGC 1 cut(s) 1813
KasI GGCGCC 1 cut(s) 1812
Kpn2I TCCGGA 1 cut(s) 126
KpnI GGTACC 1 cut(s) 724
Kzo9I GATC 8 cut(s) 259, 350, 477, 1456, 1491, 1505, 1606, 1772
LguI GCTCTTC 1 cut(s) 546
LmnI GCTCC 2 cut(s) 469, 674
LweI GCATC 3 cut(s) 74, 161, 1735
MaeI CTAG 6 cut(s) 803, 879, 1337, 1503, 1764, 1863
MaeII ACGT 2 cut(s) 774, 980
MaeIII GTNAC 3 cut(s) 429, 1113, 1295
MalI GATC 8 cut(s) 261, 352, 479, 1458, 1493, 1507, 1608, 1774
MbiI CCGCTC 1 cut(s) 464
MboI GATC 8 cut(s) 259, 350, 477, 1456, 1491, 1505, 1606, 1772
MflI RGATCY 3 cut(s) 259, 1491, 1606
Mly113I GGCGCC 1 cut(s) 1813
MlyI GAGTC 1 cut(s) 1264
MmeI TCCRAC 2 cut(s) 148, 305
Mph1103I ATGCAT 1 cut(s) 89
MroI TCCGGA 1 cut(s) 126
MseI TTAA 6 cut(s) 155, 579, 864, 947, 1317, 1356
MspA1I CMGCKG 1 cut(s) 32
MspCI CTTAAG 1 cut(s) 1355
MspI CCGG 4 cut(s) 127, 181, 1418, 1810
MspR9I CCNGG 2 cut(s) 182, 1418
Mva1269I GAATGC 1 cut(s) 541
NarI GGCGCC 1 cut(s) 1813
NciI CCSGG 2 cut(s) 182, 1418
NcoI CCATGG 1 cut(s) 209
NdeII GATC 8 cut(s) 259, 350, 477, 1456, 1491, 1505, 1606, 1772
NlaIII CATG 7 cut(s) 99, 111, 213, 608, 1181, 1465, 1571
NlaIV GGNNCC 4 cut(s) 261, 676, 722, 1814
NmuCI GTSAC 1 cut(s) 429
NsiI ATGCAT 1 cut(s) 89
NspI RCATGY 1 cut(s) 111
NspV TTCGAA 1 cut(s) 1822
PciI ACATGT 1 cut(s) 107
PciSI GCTCTTC 1 cut(s) 546
PcsI WCGNNNNNNNCGW 1 cut(s) 858
PctI GAATGC 1 cut(s) 541
PfeI GAWTC 2 cut(s) 823, 1760
PfoI TCCNGGA 1 cut(s) 180
PkrI GCNGC 1 cut(s) 1817
PleI GAGTC 1 cut(s) 1264
PluTI GGCGCC 1 cut(s) 1816
PpsI GAGTC 1 cut(s) 1264
Ppu21I YACGTR 1 cut(s) 981
PscI ACATGT 1 cut(s) 107
PsiI TTATAA 1 cut(s) 1286
PspN4I GGNNCC 4 cut(s) 261, 676, 722, 1814
PsuI RGATCY 3 cut(s) 259, 1491, 1606
RsaI GTAC 8 cut(s) 225, 722, 979, 1266, 1307, 1415, 1590, 1651
RsaNI GTAC 8 cut(s) 224, 721, 978, 1265, 1306, 1414, 1589, 1650
SapI GCTCTTC 1 cut(s) 546
SaqAI TTAA 6 cut(s) 155, 579, 864, 947, 1317, 1356
SatI GCNGC 1 cut(s) 1816
Sau3AI GATC 8 cut(s) 259, 350, 477, 1456, 1491, 1505, 1606, 1772
ScaI AGTACT 1 cut(s) 225
SchI GAGTC 1 cut(s) 1264
ScrFI CCNGG 2 cut(s) 182, 1418
SfaNI GCATC 3 cut(s) 74, 161, 1735
SfoI GGCGCC 1 cut(s) 1814
SfuI TTCGAA 1 cut(s) 1822
SmlI CTYRAG 3 cut(s) 599, 1355, 1544
SmoI CTYRAG 3 cut(s) 599, 1355, 1544
SnaBI TACGTA 1 cut(s) 981
SpeI ACTAGT 2 cut(s) 802, 878
SsiI CCGC 8 cut(s) 30, 149, 462, 750, 1094, 1615, 1748, 1816
SspDI GGCGCC 1 cut(s) 1812
SspMI CTAG 6 cut(s) 803, 879, 1337, 1503, 1764, 1863
StyD4I CCNGG 2 cut(s) 180, 1416
StyI CCWWGG 3 cut(s) 38, 209, 563
TaaI ACNGT 4 cut(s) 334, 841, 1514, 1696
TaiI ACGT 2 cut(s) 777, 983
TaqI TCGA 8 cut(s) 501, 785, 905, 1209, 1611, 1628, 1822, 1854
TatI WGTACW 1 cut(s) 223
TauI GCSGC 1 cut(s) 1818
TfiI GAWTC 2 cut(s) 823, 1760
Tru1I TTAA 6 cut(s) 155, 579, 864, 947, 1317, 1356
Tru9I TTAA 6 cut(s) 155, 579, 864, 947, 1317, 1356
TscAI CASTG 3 cut(s) 261, 1701, 1803
TseFI GTSAC 1 cut(s) 429
Tsp45I GTSAC 1 cut(s) 429
TspGWI ACGGA 2 cut(s) 374, 1490
TspRI CASTG 3 cut(s) 261, 1701, 1803
Vha464I CTTAAG 1 cut(s) 1355
XapI RAATTY 5 cut(s) 132, 393, 832, 943, 1159
XceI RCATGY 1 cut(s) 111
XmiI GTMKAC 1 cut(s) 454
XspI CTAG 6 cut(s) 803, 879, 1337, 1503, 1764, 1863
ZraI GACGTC 1 cut(s) 775
ZrmI AGTACT 1 cut(s) 225
Zsp2I ATGCAT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.