Rw0G020280

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00912
OG0005197
Physical Location & Seq
Forward (+)
89 .. 8108
8020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G020280.1

Sequence Viewer

Length: 1923 bp
ATGGCACTTTGCACTTTGCAATTTGCACAACCTATCACATCCTCAGTCTATTCCATTTCAACAATGACTCGCTCGCAAGATGAGGAAGACCCTGCTAAATCAAGGTGCTATATGAGTATGTCAGACAAGATAACAAGCCTACTTCAATGCCTATTTTCTCATGGGGGGGATACAATATTTGTAACATCTTGTGTGATTGCAGTCTCACAAGATCCTCTATTTTTTTATATTCCAACCATAAATGAGGAAGAAAAGTGCCTCGAAATGGACACCAAGTTGATGATTGTGTTACTCGTTTTGCGATCACTCACGGACATCATTTTCATATGGGACATTAAAAGTCAAAATTATAAGGCTGCAGCAATAGCTAAGACATGGCGAGCAGTTGCAACAGACTTTTTTGCTATACTTCCCGTCCCACAAGTGTTAATAACAGTCGTACTTTACAAACATCAAGAATTGAGATATCTGAATGTGTTTCTTTTCATCCAATATGTACCAAGGATTTATCGACTTAGTCAATCGTTTGACAATCTTAAGCGGAGAAGGGACACCAGATTATGGGTCAAAGTTGCATTCAATTTTTTTCTATACATCCTTGCTTGTCATATACTTGGAGCCTCGTGGTTTTTCTTTTCTGTTCAACGAGAGATGTTTTGTTGGCGCCAAACTTGTGTAGAACAGAAGACATGCAGAAGTAATATCAAGTGCAACGAAAATAGTTCGAGAAATGTCACAAAACTAGATCAACTTTGTCCAATGAATCCACCAAATTCTACCATATTTGATTTTGGGATATTTGTTAATACTCTGGGAACTAGTCGCAGAACACCAATAAACTTCTCGACGAAGTTATCTTACTCTTTCTGGTGGGGAGTGCGAAATATAAGTAATTTTGGCACAAATCTGGAAACAAGTACTTATTTGGGGGAAGTTTGGTTTGCAACTCTAATTTCGGTTATCGGCTTGCTACTCTTTGTATATCTCATTGGAAATAATGTTCAGACATTCATGCAAATGAAAACTATGAAAGAGGAGGAGGAAGCTAAAAAGATGAGGCAAGAAGCTGAAAGGATAAGGCAAGAGCTTCTCCATCAAGAGAGACGCATAACTTCGTGGATGCACCGACTTAATTTCCCTGGGTTTTTGAGGGAAGAAGTCGAGAGAAAAATTAAAGAGAAGTTGCAAGAAAACAAAGAGGTTAATCTTGCTAATGTATTGACTCTATTATCATTTATGGATTCGGACCTCCACAGAGATGTAAAGTGGCTGATACTGGAAAATGTGCCAGTCATTAAAGACATGGGTCGGCGTGTGTTGTCTGAAATTTCTGAGTTGATGGAGATAGTGGAGTACCCCGAAAATACTTTGATTGTTGAAAGGGCCGGACCCTTGGATCGCATGGTTGTCATTACAGAAGGGACTATGGGGGTCTACAAGACTAGCACGAACCCTTCTCCATCAGCAATCTTGAATGGGTCTTTCGACCAAGTGAATGTCTACGGACAGATACAACTAGTGAGTTGGGCACTCTCAACCAACGGAAATCTCGACAGCCTTCCTATCTCGGAAGAAACCGTCAAGTGTCATACCAAAGTAGAAGGATTTGTTCTCACAGCCAGTGACTTGAAGAAGGTCCTCTCTAAATACGGCGTAATCCAAGACAATGATTCTGATCAAGATCATGTGGAAATTAGTAATTCCATTGAGGGGGAAAGTTCTTCTAGTATGGGATCAGCAATCCTTGTAGCAACAGATCAAGATCATGGGGAAATTAGTAATTCTATTGAGGGGGAAAGTTCTTCTAGCCTGAGATCAGCAATTCTTGTAGCAACAACAGCCTCTCGTTTCCGACGACGCCTTCGAAGAGCGAAGCCTGCATTACATTTAGATTTCGACCCCTCTAGCTACAAAGGAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

640

Amino Acids

73.28

Weight (kDa)

6.59

Isoelectric Point (pI)

52.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 63 - 342 1.3e-09 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 351
AccB1I GGYRCC 1 cut(s) 663
AccB7I CCANNNNNTGG 1 cut(s) 561
AccI GTMKAC 2 cut(s) 1434, 1500
AciI CCGC 1 cut(s) 541
AclWI GGATC 3 cut(s) 206, 1404, 1741
AcsI RAATTY 2 cut(s) 772, 1326
AcyI GRCGYC 2 cut(s) 664, 1858
AfaI GTAC 4 cut(s) 441, 498, 919, 1355
AfiI CCNNNNNNNGG 3 cut(s) 265, 561, 1710
AflII CTTAAG 1 cut(s) 536
AgsI TTSAA 7 cut(s) 60, 146, 580, 644, 1379, 1474, 1630
AhlI ACTAGT 2 cut(s) 818, 1516
AjnI CCWGG 1 cut(s) 1138
AloI GAACNNNNNNTCC 2 cut(s) 984, 1016
AluBI AGCT 5 cut(s) 368, 1046, 1067, 1087, 1908
AluI AGCT 5 cut(s) 368, 1046, 1067, 1087, 1908
Alw26I GTCTC 2 cut(s) 208, 1096
AlwI GGATC 3 cut(s) 206, 1404, 1741
AoxI GGCC 1 cut(s) 1383
ApeKI GCWGC 2 cut(s) 356, 359
ApoI RAATTY 2 cut(s) 772, 1326
ArsI GACNNNNNNTTYG 2 cut(s) 1845, 1877
AspLEI GCGC 1 cut(s) 666
AspS9I GGNCC 4 cut(s) 1246, 1383, 1388, 1636
AsuII TTCGAA 1 cut(s) 1864
AvaII GGWCC 3 cut(s) 1246, 1388, 1636
BaeGI GKGCMC 1 cut(s) 1531
BanI GGYRCC 1 cut(s) 663
BarI GAAGNNNNNNTAC 2 cut(s) 842, 874
BauI CACGAG 1 cut(s) 622
BbsI GAAGAC 2 cut(s) 93, 692
BbvI GCAGC 2 cut(s) 343, 371
BccI CCATC 3 cut(s) 1101, 1333, 1468
BceAI ACGGC 1 cut(s) 1666
BciT130I CCWGG 1 cut(s) 1140
BciVI GTATCC 1 cut(s) 163
BclI TGATCA 1 cut(s) 1675
BcoDI GTCTC 2 cut(s) 208, 1096
BcuI ACTAGT 2 cut(s) 818, 1516
BfaI CTAG 7 cut(s) 743, 819, 1443, 1517, 1725, 1806, 1905
BfmI CTRYAG 1 cut(s) 357
BfoI RGCGCY 1 cut(s) 667
BfrI CTTAAG 1 cut(s) 536
BfuI GTATCC 1 cut(s) 163
BisI GCNGC 2 cut(s) 357, 360
BlsI GCNGC 2 cut(s) 358, 361
BmcAI AGTACT 1 cut(s) 919
Bme1390I CCNGG 1 cut(s) 1140
Bme18I GGWCC 3 cut(s) 1246, 1388, 1636
BmgT120I GGNCC 4 cut(s) 1246, 1383, 1388, 1636
BmiI GGNNCC 3 cut(s) 619, 665, 1390
BmrFI CCNGG 1 cut(s) 1140
BmsI GCATC 1 cut(s) 1110
BoxI GACNNNNGTC 1 cut(s) 1305
BpiI GAAGAC 2 cut(s) 93, 692
Bpu14I TTCGAA 1 cut(s) 1864
BsaBI GATNNNNATC 3 cut(s) 1674, 1680, 1761
BsaHI GRCGYC 2 cut(s) 664, 1858
BsaJI CCNNGG 4 cut(s) 500, 1138, 1139, 1392
Bsc4I CCNNNNNNNGG 3 cut(s) 265, 561, 1710
Bse1I ACTGG 3 cut(s) 1281, 1289, 1620
Bse8I GATNNNNATC 3 cut(s) 1674, 1680, 1761
BseBI CCWGG 1 cut(s) 1140
BseDI CCNNGG 4 cut(s) 500, 1138, 1139, 1392
BseGI GGATG 4 cut(s) 38, 486, 594, 1125
BseJI GATNNNNATC 3 cut(s) 1674, 1680, 1761
BseLI CCNNNNNNNGG 3 cut(s) 265, 561, 1710
BseMII CTCAG 3 cut(s) 57, 1323, 1802
BseNI ACTGG 3 cut(s) 1281, 1289, 1620
BseRI GAGGAG 2 cut(s) 1049, 1052
BseSI GKGCMC 1 cut(s) 1531
BseXI GCAGC 2 cut(s) 343, 371
BshFI GGCC 1 cut(s) 1385
BshNI GGYRCC 1 cut(s) 663
BsiSI CCGG 1 cut(s) 1386
BslFI GGGAC 4 cut(s) 344, 401, 563, 1435
BslI CCNNNNNNNGG 3 cut(s) 265, 561, 1710
BsmAI GTCTC 2 cut(s) 208, 1096
BsmBI CGTCTC 1 cut(s) 1096
BsmFI GGGAC 4 cut(s) 344, 401, 563, 1435
BsmI GAATGC 1 cut(s) 575
BsnI GGCC 1 cut(s) 1385
Bsp119I TTCGAA 1 cut(s) 1864
Bsp1286I GDGCHC 1 cut(s) 1531
BspACI CCGC 1 cut(s) 541
BspANI GGCC 1 cut(s) 1385
BspCNI CTCAG 3 cut(s) 56, 1324, 1803
BspLI GGNNCC 3 cut(s) 619, 665, 1390
BspMAI CTGCAG 1 cut(s) 361
BspPI GGATC 3 cut(s) 206, 1404, 1741
BspQI GCTCTTC 1 cut(s) 1861
BspT104I TTCGAA 1 cut(s) 1864
BspT107I GGYRCC 1 cut(s) 663
BspTI CTTAAG 1 cut(s) 536
BsrI ACTGG 3 cut(s) 1281, 1289, 1620
BssECI CCNNGG 4 cut(s) 500, 1138, 1139, 1392
BssNI GRCGYC 2 cut(s) 664, 1858
BssSI CACGAG 1 cut(s) 622
BssT1I CCWWGG 2 cut(s) 500, 1392
Bst2BI CACGAG 1 cut(s) 622
Bst2UI CCWGG 1 cut(s) 1140
Bst4CI ACNGT 2 cut(s) 436, 1579
Bst6I CTCTTC 1 cut(s) 1861
BstACI GRCGYC 2 cut(s) 664, 1858
BstAFI CTTAAG 1 cut(s) 536
BstBI TTCGAA 1 cut(s) 1864
BstC8I GCNNGC 4 cut(s) 74, 381, 968, 1878
BstDEI CTNAG 5 cut(s) 43, 369, 515, 1332, 1811
BstF5I GGATG 4 cut(s) 38, 486, 594, 1125
BstH2I RGCGCY 1 cut(s) 667
BstHHI GCGC 1 cut(s) 666
BstMAI GTCTC 2 cut(s) 208, 1096
BstMWI GCNNNNNNNGC 3 cut(s) 365, 1838, 1877
BstNI CCWGG 1 cut(s) 1140
BstNSI RCATGY 1 cut(s) 693
BstPAI GACNNNNGTC 1 cut(s) 1305
BstSCI CCNGG 1 cut(s) 1138
BstSFI CTRYAG 1 cut(s) 357
BstSLI GKGCMC 1 cut(s) 1531
BstV1I GCAGC 2 cut(s) 343, 371
BstV2I GAAGAC 2 cut(s) 93, 692
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BsuI GTATCC 1 cut(s) 163
BsuRI GGCC 1 cut(s) 1385
BtsCI GGATG 4 cut(s) 38, 486, 594, 1125
BtsIMutI CAGTG 1 cut(s) 1627
Cac8I GCNNGC 4 cut(s) 74, 381, 968, 1878
CfoI GCGC 1 cut(s) 666
Cfr13I GGNCC 4 cut(s) 1246, 1383, 1388, 1636
CseI GACGC 2 cut(s) 1113, 1866
Csp6I GTAC 4 cut(s) 440, 497, 918, 1354
CviAII CATG 8 cut(s) 161, 375, 690, 1012, 1303, 1402, 1685, 1766
CviQI GTAC 4 cut(s) 440, 497, 918, 1354
DdeI CTNAG 5 cut(s) 43, 369, 515, 1332, 1811
DinI GGCGCC 1 cut(s) 665
Eam1104I CTCTTC 1 cut(s) 1861
EarI CTCTTC 1 cut(s) 1861
Eco130I CCWWGG 2 cut(s) 500, 1392
Eco32I GATATC 1 cut(s) 467
Eco47I GGWCC 3 cut(s) 1246, 1388, 1636
EcoO109I RGGNCCY 1 cut(s) 1636
EcoRII CCWGG 1 cut(s) 1138
EcoRV GATATC 1 cut(s) 467
EcoT14I CCWWGG 2 cut(s) 500, 1392
EgeI GGCGCC 1 cut(s) 665
EheI GGCGCC 1 cut(s) 665
ErhI CCWWGG 2 cut(s) 500, 1392
Esp3I CGTCTC 1 cut(s) 1096
FaeI CATG 8 cut(s) 164, 378, 693, 1015, 1306, 1405, 1688, 1769
FaqI GGGAC 4 cut(s) 344, 401, 563, 1435
FatI CATG 8 cut(s) 160, 374, 689, 1011, 1302, 1401, 1684, 1765
FauNDI CATATG 1 cut(s) 326
FbaI TGATCA 1 cut(s) 1675
FblI GTMKAC 2 cut(s) 1434, 1500
Fnu4HI GCNGC 2 cut(s) 357, 360
FokI GGATG 4 cut(s) 25, 473, 581, 1132
Fsp4HI GCNGC 2 cut(s) 357, 360
FspBI CTAG 7 cut(s) 743, 819, 1443, 1517, 1725, 1806, 1905
GlaI GCGC 1 cut(s) 665
GluI GCNGC 2 cut(s) 357, 360
HaeII RGCGCY 1 cut(s) 667
HaeIII GGCC 1 cut(s) 1385
HapII CCGG 1 cut(s) 1386
HgaI GACGC 2 cut(s) 1113, 1866
HhaI GCGC 1 cut(s) 666
Hin1I GRCGYC 2 cut(s) 664, 1858
Hin1II CATG 8 cut(s) 164, 378, 693, 1015, 1306, 1405, 1688, 1769
Hin6I GCGC 1 cut(s) 664
HinP1I GCGC 1 cut(s) 664
HinfI GANTC 5 cut(s) 67, 763, 1222, 1241, 1670
HpaII CCGG 1 cut(s) 1386
Hpy166II GTNNAC 2 cut(s) 1435, 1501
Hpy188I TCNGA 9 cut(s) 124, 471, 1005, 1246, 1324, 1333, 1570, 1675, 1853
Hpy8I GTNNAC 2 cut(s) 1435, 1501
Hpy99I CGWCG 3 cut(s) 850, 1857, 1860
HpyAV CCTTC 7 cut(s) 540, 1412, 1464, 1568, 1595, 1627, 1871
HpyCH4III ACNGT 2 cut(s) 436, 1579
HpyF10VI GCNNNNNNNGC 3 cut(s) 365, 1838, 1877
HpyF3I CTNAG 5 cut(s) 43, 369, 515, 1332, 1811
Hsp92I GRCGYC 2 cut(s) 664, 1858
Hsp92II CATG 8 cut(s) 164, 378, 693, 1015, 1306, 1405, 1688, 1769
HspAI GCGC 1 cut(s) 664
KasI GGCGCC 1 cut(s) 663
Ksp22I TGATCA 1 cut(s) 1675
LguI GCTCTTC 1 cut(s) 1861
LmnI GCTCC 1 cut(s) 617
Lsp1109I GCAGC 2 cut(s) 343, 371
LweI GCATC 1 cut(s) 1110
MaeI CTAG 7 cut(s) 743, 819, 1443, 1517, 1725, 1806, 1905
MaeIII GTNAC 4 cut(s) 181, 288, 733, 1622
MboII GAAGA 9 cut(s) 98, 260, 697, 1166, 1583, 1642, 1713, 1794, 1878
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 1 cut(s) 1531
Mly113I GGCGCC 1 cut(s) 664
MlyI GAGTC 2 cut(s) 61, 1216
MmeI TCCRAC 2 cut(s) 257, 1876
MseI TTAA 8 cut(s) 336, 428, 537, 804, 1131, 1173, 1203, 1296
MslI CAYNNNNRTG 2 cut(s) 1016, 1257
MspCI CTTAAG 1 cut(s) 536
MspI CCGG 1 cut(s) 1386
MspR9I CCNGG 1 cut(s) 1140
Mva1269I GAATGC 1 cut(s) 575
MvaI CCWGG 1 cut(s) 1140
MwoI GCNNNNNNNGC 3 cut(s) 365, 1838, 1877
NarI GGCGCC 1 cut(s) 664
NdeI CATATG 1 cut(s) 326
NlaIII CATG 8 cut(s) 164, 378, 693, 1015, 1306, 1405, 1688, 1769
NlaIV GGNNCC 3 cut(s) 619, 665, 1390
NmuCI GTSAC 2 cut(s) 733, 1622
NspI RCATGY 1 cut(s) 693
NspV TTCGAA 1 cut(s) 1864
PasI CCCWGGG 1 cut(s) 1139
PciSI GCTCTTC 1 cut(s) 1861
PcsI WCGNNNNNNNCGW 3 cut(s) 1548, 1852, 1861
PctI GAATGC 1 cut(s) 575
PfeI GAWTC 3 cut(s) 763, 1241, 1670
PflFI GACNNNGTC 1 cut(s) 516
PflMI CCANNNNNTGG 1 cut(s) 561
PkrI GCNGC 2 cut(s) 358, 361
PleI GAGTC 2 cut(s) 61, 1216
PluTI GGCGCC 1 cut(s) 667
PpsI GAGTC 2 cut(s) 61, 1216
PpuMI RGGWCCY 1 cut(s) 1636
PshAI GACNNNNGTC 1 cut(s) 1305
PsiI TTATAA 1 cut(s) 351
Psp5II RGGWCCY 1 cut(s) 1636
Psp6I CCWGG 1 cut(s) 1138
PspGI CCWGG 1 cut(s) 1138
PspN4I GGNNCC 3 cut(s) 619, 665, 1390
PspPI GGNCC 4 cut(s) 1246, 1383, 1388, 1636
PspPPI RGGWCCY 1 cut(s) 1636
PstI CTGCAG 1 cut(s) 361
PsuI RGATCY 1 cut(s) 211
PsyI GACNNNGTC 1 cut(s) 516
RsaI GTAC 4 cut(s) 441, 498, 919, 1355
RsaNI GTAC 4 cut(s) 440, 497, 918, 1354
RseI CAYNNNNRTG 2 cut(s) 1016, 1257
SapI GCTCTTC 1 cut(s) 1861
SaqAI TTAA 8 cut(s) 336, 428, 537, 804, 1131, 1173, 1203, 1296
SatI GCNGC 2 cut(s) 357, 360
Sau96I GGNCC 4 cut(s) 1246, 1383, 1388, 1636
ScaI AGTACT 1 cut(s) 919
SchI GAGTC 2 cut(s) 61, 1216
ScrFI CCNGG 1 cut(s) 1140
SduI GDGCHC 1 cut(s) 1531
SfaNI GCATC 1 cut(s) 1110
SfcI CTRYAG 1 cut(s) 357
SfoI GGCGCC 1 cut(s) 665
SfuI TTCGAA 1 cut(s) 1864
SinI GGWCC 3 cut(s) 1246, 1388, 1636
SmiMI CAYNNNNRTG 2 cut(s) 1016, 1257
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
SpeI ACTAGT 2 cut(s) 818, 1516
SsiI CCGC 1 cut(s) 541
SspDI GGCGCC 1 cut(s) 663
SspI AATATT 1 cut(s) 177
SspMI CTAG 7 cut(s) 743, 819, 1443, 1517, 1725, 1806, 1905
StyD4I CCNGG 1 cut(s) 1138
StyI CCWWGG 2 cut(s) 500, 1392
TaaI ACNGT 2 cut(s) 436, 1579
TaqI TCGA 9 cut(s) 261, 511, 725, 845, 1161, 1485, 1551, 1864, 1896
TatI WGTACW 1 cut(s) 917
TfiI GAWTC 3 cut(s) 763, 1241, 1670
Tru1I TTAA 8 cut(s) 336, 428, 537, 804, 1131, 1173, 1203, 1296
Tru9I TTAA 8 cut(s) 336, 428, 537, 804, 1131, 1173, 1203, 1296
TscAI CASTG 1 cut(s) 1627
TseFI GTSAC 2 cut(s) 733, 1622
TseI GCWGC 2 cut(s) 356, 359
Tsp45I GTSAC 2 cut(s) 733, 1622
TspDTI ATGAA 6 cut(s) 313, 475, 776, 1000, 1034, 1043
TspGWI ACGGA 3 cut(s) 326, 1518, 1557
TspRI CASTG 1 cut(s) 1627
Tth111I GACNNNGTC 1 cut(s) 516
Van91I CCANNNNNTGG 1 cut(s) 561
Vha464I CTTAAG 1 cut(s) 536
VpaK11BI GGWCC 3 cut(s) 1246, 1388, 1636
XapI RAATTY 2 cut(s) 772, 1326
XceI RCATGY 1 cut(s) 693
XmiI GTMKAC 2 cut(s) 1434, 1500
XspI CTAG 7 cut(s) 743, 819, 1443, 1517, 1725, 1806, 1905
ZrmI AGTACT 1 cut(s) 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.