Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0002181

Physical Location & Seq

Genomic Coordinates Reverse (-)

18969246 .. 18972323

Gene Structure 3078 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g24590.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1299 bp

ATGGCGAGTTGGGTCATCTCAGAATGTGGCCTCAAGCCTCTCCCTAGAGTCTACCCTCAAACCAGAAATGGCCTCACCTCAAAACCCATTAGGCCATTGCAACTCAAAAACAAGAGCCTTGGTTCTTCTTTGAGGGTGTCAAATGGGTTTAGAGGGCAGAGTTGGGCGCTCAATGTGAGTGCTCCATTGAATGTGGTGACTCTTGGGGAAGATAGGGTTGATCAAGATGGTGGGGAGTTTGACCCTGGTGCAGCTCCTCCGTTTAATTTGGGTGATATTCGGGCAGCAATTCCAAAGCATTGTTGGGTTAAGAATCCTTGGAAGTCTATGAGTTATGTTGTGAGGGATGTGGCTGTGGTTTTTGGCTTGGCGGCTGTGGCGGCTTACTTTAACAATTGGGCTGTTTGGCCTCTTTACTGGTTTGCTCAGAGCACCATGTTCTGGGCTCTCTTTGTTCTTGGCCATGATTGTGGTCATGGAAGCTTCTCTAATAACCCAAAGCTGAATAGTGTGGTGGGTCATCTTCTTCATTCTTCAATCCTTGTCCCATACCATGGATGGAGAATAAGTCACAGAACTCATCATCAGAACCATGGTCATGTCGAAAATGATGAGTCTTGGCATCCGTTAACTGAGAAAACCTTCAAAAGTTTGGATAAGGTTACTCAGATATTGAGGTTCGCCTTGCCTTTCCCTATGCTTGCTTACCCCTTTTATCTGTGGCTTAGAAGTCCAGGAAAGAAAGGTTCTCATTATAATCCGAGCAGCGACTTGTTTCTTCCAACTGAAAGGAAAGATATTATCACCTCCACTACCTGTTGGACTGCAATGGTTGCCCTGCTTATTGGTCTGAACTTTGTAATGGGTCCAATGCAAATGCTTAAGCTTTATGGAATTCCATACTGGGGGTTTGTCGTATGGTTGGACTTAGTGACTTACTTACATCACCATGGCCATGAAGACAAGCTTCCATGGTATCGTGGAAAGGAATGGAGTTATCTAAGAGGAGGGCTAACAACCCTTGATCGTGACTATGGACTGTTCAATAACATTCATCATGATATTGGTACCCATGTCATACATCACCTATTCCCTCAAATCCCACACTACAACTTAGTAGAAGCAACCGAGGCAGCTAAGCCGGTGTTTGGGAAGTACTACAGGGAGCCAAAGAAATCTGGGCCTCTCCCATTCCATTTATTGGGAGTCCTTGTAAAAAGCTTCAAGAAAGATCACTATGTAAGTGATACTGGGGATGTTGTATATTATCAAACTGATAAGAAAATCTCCGGATCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006950 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009536 GO:0009628 GO:0009941 GO:0009987 GO:0016020 GO:0016053 GO:0016491 GO:0016705 GO:0019752 GO:0031090 GO:0031967 GO:0031975 GO:0032787 GO:0042170 GO:0042389 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0055114 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3474 FA_desaturase

Protein Analysis

433

Amino Acids

49.17

Weight (kDa)

9.28

Isoelectric Point (pI)

29.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF3474	PF11960	1 - 125	6.9e-48	Domain of unknown function (DUF3474)
FA_desaturase	PF00487	133 - 387	2.3e-30	Fatty acid desaturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002181)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11170 AT5G05580 AT5G05580
fragaria_vesca	FvH4_6g11310 FvH4_6g11310 FvH4_6g11310 FvH4_6g11310 FvH4_7g24590 FvH4_7g24590 FvH4_7g24590
malus_domestica	MD01G1154100.v1.1 MD04G1155400.v1.1 MD07G1223100.v1.1 MD12G1168600.v1.1 MD17G1258700.v1.1
prunus_persica	Prupe.2G251200_v2.0.a1 Prupe.6G278800_v2.0.a1 Prupe.6G278800_v2.0.a1
pyrus_communis	pycom01g17140 pycom04g13980 pycom07g20170 pycom12g16010
rosa_chinensis	RchiOBHm_Chr1g0370421 RchiOBHm_Chr3g0462851
rosa_laevigata	RLG00000024827 RLG00000027049
rosa_multiflora	Rmu_sc0000766.1_g000013 Rmu_sc0005656.1_g000006
rosa_roxburghii	Rroxscaffold_4G00286590 Rroxscaffold_6G00417140
rosa_rugosa	Rorug01G0356300 Rorug03G0059600
rosa_samantha	Rh1AG364800 Rh1BG328000 Rh1CG341000 Rh1DG358300 Rh3AG117800 Rh3BG121600 Rh3CG124500 Rh3DG123200
rosa_wichuraiana	Rw1G032100 Rw1G032150 Rw3G009990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	756
Acc65I	GGTACC	1 cut(s)	1067
AccB1I	GGYRCC	1 cut(s)	1067
AccB7I	CCANNNNNTGG	3 cut(s)	441, 554, 1201
AccI	GTMKAC	1 cut(s)	51
AccIII	TCCGGA	1 cut(s)	1289
AciI	CCGC	2 cut(s)	371, 380
AcoI	YGGCCR	2 cut(s)	460, 952
AcsI	RAATTY	1 cut(s)	894
AfaI	GTAC	2 cut(s)	1069, 1157
AfiI	CCNNNNNNNGG	5 cut(s)	441, 554, 905, 1148, 1201
AflII	CTTAAG	1 cut(s)	881
AgsI	TTSAA	5 cut(s)	190, 537, 646, 1045, 1225
AjnI	CCWGG	2 cut(s)	244, 733
AluBI	AGCT	7 cut(s)	254, 483, 502, 886, 967, 1136, 1221
AluI	AGCT	7 cut(s)	254, 483, 502, 886, 967, 1136, 1221
Alw21I	GWGCWC	2 cut(s)	184, 434
Aor13HI	TCCGGA	1 cut(s)	1289
AoxI	GGCC	7 cut(s)	28, 70, 92, 407, 460, 952, 1181
ApeKI	GCWGC	4 cut(s)	251, 284, 765, 1133
ApoI	RAATTY	1 cut(s)	894
Asp700I	GAANNNNTTC	1 cut(s)	641
Asp718I	GGTACC	1 cut(s)	1067
AspLEI	GCGC	1 cut(s)	169
AspS9I	GGNCC	2 cut(s)	866, 1181
AsuHPI	GGTGA	6 cut(s)	67, 208, 284, 796, 938, 1076
AvaII	GGWCC	1 cut(s)	866
BaeI	ACNNNNGTAYC	2 cut(s)	1059, 1092
BalI	TGGCCA	2 cut(s)	462, 954
BanI	GGYRCC	1 cut(s)	1067
BanII	GRGCYC	1 cut(s)	448
BbsI	GAAGAC	1 cut(s)	966
Bbv12I	GWGCWC	2 cut(s)	184, 434
BbvI	GCAGC	4 cut(s)	263, 296, 777, 1145
BccI	CCATC	2 cut(s)	221, 552
BciT130I	CCWGG	2 cut(s)	246, 735
BclI	TGATCA	1 cut(s)	220
BfaI	CTAG	1 cut(s)	45
BfmI	CTRYAG	1 cut(s)	1159
BfoI	RGCGCY	1 cut(s)	170
BfrI	CTTAAG	1 cut(s)	881
BisI	GCNGC	6 cut(s)	252, 285, 372, 381, 766, 1134
BlpI	GCTNAGC	1 cut(s)	1137
BlsI	GCNGC	6 cut(s)	253, 286, 373, 382, 767, 1135
BmcAI	AGTACT	1 cut(s)	1157
Bme1390I	CCNGG	2 cut(s)	246, 735
Bme18I	GGWCC	1 cut(s)	866
BmgT120I	GGNCC	2 cut(s)	866, 1181
BmiI	GGNNCC	3 cut(s)	867, 1069, 1167
BmrFI	CCNGG	2 cut(s)	246, 735
BmrI	ACTGGG	2 cut(s)	913, 1260
BmsI	GCATC	1 cut(s)	631
BmuI	ACTGGG	2 cut(s)	913, 1260
BpiI	GAAGAC	1 cut(s)	966
Bpu1102I	GCTNAGC	1 cut(s)	1137
BpuEI	CTTGAG	1 cut(s)	17
BsaJI	CCNNGG	8 cut(s)	118, 244, 317, 553, 592, 949, 971, 1128
BsaWI	WCCGGW	1 cut(s)	1289
BsaXI	ACNNNNNCTCC	4 cut(s)	553, 583, 1197, 1227
Bsc4I	CCNNNNNNNGG	5 cut(s)	441, 554, 905, 1148, 1201
Bse118I	RCCGGY	1 cut(s)	1141
Bse1I	ACTGG	3 cut(s)	422, 908, 1255
Bse3DI	GCAATG	2 cut(s)	95, 834
BseAI	TCCGGA	1 cut(s)	1289
BseBI	CCWGG	2 cut(s)	246, 735
BseDI	CCNNGG	8 cut(s)	118, 244, 317, 553, 592, 949, 971, 1128
BseGI	GGATG	4 cut(s)	352, 563, 622, 1261
BseLI	CCNNNNNNNGG	5 cut(s)	441, 554, 905, 1148, 1201
BseMI	GCAATG	2 cut(s)	95, 834
BseMII	CTCAG	4 cut(s)	33, 440, 624, 680
BseNI	ACTGG	3 cut(s)	422, 908, 1255
BseRI	GAGGAG	2 cut(s)	246, 1020
BseXI	GCAGC	4 cut(s)	263, 296, 777, 1145
BsgI	GTGCAG	1 cut(s)	270
BshFI	GGCC	7 cut(s)	30, 72, 94, 409, 462, 954, 1183
BshNI	GGYRCC	1 cut(s)	1067
BsiHKAI	GWGCWC	2 cut(s)	184, 434
BsiSI	CCGG	2 cut(s)	1142, 1290
BslFI	GGGAC	1 cut(s)	530
BslI	CCNNNNNNNGG	5 cut(s)	441, 554, 905, 1148, 1201
BsmFI	GGGAC	1 cut(s)	530
BsnI	GGCC	7 cut(s)	30, 72, 94, 409, 462, 954, 1183
Bsp1286I	GDGCHC	3 cut(s)	184, 434, 448
Bsp13I	TCCGGA	1 cut(s)	1289
Bsp143I	GATC	4 cut(s)	220, 1024, 1231, 1292
Bsp1720I	GCTNAGC	1 cut(s)	1137
Bsp19I	CCATGG	4 cut(s)	553, 592, 949, 971
BspACI	CCGC	2 cut(s)	371, 380
BspANI	GGCC	7 cut(s)	30, 72, 94, 409, 462, 954, 1183
BspCNI	CTCAG	4 cut(s)	32, 439, 625, 679
BspEI	TCCGGA	1 cut(s)	1289
BspHI	TCATGA	1 cut(s)	1057
BspLI	GGNNCC	3 cut(s)	867, 1069, 1167
BspT107I	GGYRCC	1 cut(s)	1067
BspTI	CTTAAG	1 cut(s)	881
BsrDI	GCAATG	2 cut(s)	95, 834
BsrFI	RCCGGY	1 cut(s)	1141
BsrI	ACTGG	3 cut(s)	422, 908, 1255
BssAI	RCCGGY	1 cut(s)	1141
BssECI	CCNNGG	8 cut(s)	118, 244, 317, 553, 592, 949, 971, 1128
BssMI	GATC	4 cut(s)	220, 1024, 1231, 1292
BssT1I	CCWWGG	6 cut(s)	118, 317, 553, 592, 949, 971
Bst2UI	CCWGG	2 cut(s)	246, 735
Bst4CI	ACNGT	1 cut(s)	1041
BstAFI	CTTAAG	1 cut(s)	881
BstAPI	GCANNNNNTGC	1 cut(s)	833
BstC8I	GCNNGC	1 cut(s)	702
BstDSI	CCRYGG	4 cut(s)	553, 592, 949, 971
BstF5I	GGATG	4 cut(s)	352, 563, 622, 1261
BstH2I	RGCGCY	1 cut(s)	170
BstHHI	GCGC	1 cut(s)	169
BstKTI	GATC	4 cut(s)	223, 1027, 1234, 1295
BstMBI	GATC	4 cut(s)	220, 1024, 1231, 1292
BstMWI	GCNNNNNNNGC	4 cut(s)	377, 380, 833, 1130
BstNI	CCWGG	2 cut(s)	246, 735
BstSCI	CCNGG	2 cut(s)	244, 733
BstSFI	CTRYAG	1 cut(s)	1159
BstV1I	GCAGC	4 cut(s)	263, 296, 777, 1145
BstV2I	GAAGAC	1 cut(s)	966
BstX2I	RGATCY	1 cut(s)	1292
BstXI	CCANNNNNNTGG	1 cut(s)	470
BstYI	RGATCY	1 cut(s)	1292
BsuRI	GGCC	7 cut(s)	30, 72, 94, 409, 462, 954, 1183
BtgI	CCRYGG	4 cut(s)	553, 592, 949, 971
BtsCI	GGATG	4 cut(s)	352, 563, 622, 1261
Cac8I	GCNNGC	1 cut(s)	702
CciI	TCATGA	1 cut(s)	1057
CfoI	GCGC	1 cut(s)	169
Cfr10I	RCCGGY	1 cut(s)	1141
Cfr13I	GGNCC	2 cut(s)	866, 1181
Csp6I	GTAC	2 cut(s)	1068, 1156
CviQI	GTAC	2 cut(s)	1068, 1156
DpnI	GATC	4 cut(s)	222, 1026, 1233, 1294
DpnII	GATC	4 cut(s)	220, 1024, 1231, 1292
EaeI	YGGCCR	2 cut(s)	460, 952
Eco130I	CCWWGG	6 cut(s)	118, 317, 553, 592, 949, 971
Eco24I	GRGCYC	1 cut(s)	448
Eco47I	GGWCC	1 cut(s)	866
EcoRI	GAATTC	1 cut(s)	894
EcoRII	CCWGG	2 cut(s)	244, 733
EcoT14I	CCWWGG	6 cut(s)	118, 317, 553, 592, 949, 971
EcoT38I	GRGCYC	1 cut(s)	448
ErhI	CCWWGG	6 cut(s)	118, 317, 553, 592, 949, 971
FalI	AAGNNNNNCTT	2 cut(s)	951, 983
FaqI	GGGAC	1 cut(s)	530
FbaI	TGATCA	1 cut(s)	220
FblI	GTMKAC	1 cut(s)	51
Fnu4HI	GCNGC	6 cut(s)	252, 285, 372, 381, 766, 1134
FokI	GGATG	4 cut(s)	359, 570, 609, 1268
FriOI	GRGCYC	1 cut(s)	448
Fsp4HI	GCNGC	6 cut(s)	252, 285, 372, 381, 766, 1134
FspBI	CTAG	1 cut(s)	45
GlaI	GCGC	1 cut(s)	168
GluI	GCNGC	6 cut(s)	252, 285, 372, 381, 766, 1134
HaeII	RGCGCY	1 cut(s)	170
HaeIII	GGCC	7 cut(s)	30, 72, 94, 409, 462, 954, 1183
HapII	CCGG	2 cut(s)	1142, 1290
HhaI	GCGC	1 cut(s)	169
Hin6I	GCGC	1 cut(s)	167
HinP1I	GCGC	1 cut(s)	167
HincII	GTYRAC	1 cut(s)	630
HindII	GTYRAC	1 cut(s)	630
HindIII	AAGCTT	4 cut(s)	481, 884, 965, 1219
HinfI	GANTC	5 cut(s)	48, 199, 313, 614, 1206
HpaI	GTTAAC	1 cut(s)	630
HpaII	CCGG	2 cut(s)	1142, 1290
HphI	GGTGA	6 cut(s)	67, 208, 284, 796, 938, 1076
Hpy166II	GTNNAC	2 cut(s)	52, 630
Hpy188I	TCNGA	6 cut(s)	22, 429, 588, 669, 762, 852
Hpy188III	TCNNGA	5 cut(s)	224, 1028, 1058, 1225, 1290
Hpy8I	GTNNAC	2 cut(s)	52, 630
HpyAV	CCTTC	1 cut(s)	652
HpyCH4III	ACNGT	1 cut(s)	1041
HpyCH4V	TGCA	4 cut(s)	100, 251, 827, 874
HpyF10VI	GCNNNNNNNGC	4 cut(s)	377, 380, 833, 1130
HspAI	GCGC	1 cut(s)	167
Kpn2I	TCCGGA	1 cut(s)	1289
KpnI	GGTACC	1 cut(s)	1071
Ksp22I	TGATCA	1 cut(s)	220
KspAI	GTTAAC	1 cut(s)	630
Kzo9I	GATC	4 cut(s)	220, 1024, 1231, 1292
LmnI	GCTCC	3 cut(s)	187, 259, 1165
Lsp1109I	GCAGC	4 cut(s)	263, 296, 777, 1145
LweI	GCATC	1 cut(s)	631
MaeI	CTAG	1 cut(s)	45
MaeIII	GTNAC	5 cut(s)	196, 569, 661, 931, 1028
MalI	GATC	4 cut(s)	222, 1026, 1233, 1294
MboI	GATC	4 cut(s)	220, 1024, 1231, 1292
MboII	GAAGA	7 cut(s)	117, 221, 515, 518, 525, 770, 971
MfeI	CAATTG	1 cut(s)	394
MflI	RGATCY	1 cut(s)	1292
MhlI	GDGCHC	3 cut(s)	184, 434, 448
MlsI	TGGCCA	2 cut(s)	462, 954
MluCI	AATT	4 cut(s)	265, 288, 394, 894
MluNI	TGGCCA	2 cut(s)	462, 954
MlyI	GAGTC	4 cut(s)	57, 193, 623, 1215
MmeI	TCCRAC	3 cut(s)	800, 806, 903
Mox20I	TGGCCA	2 cut(s)	462, 954
MroI	TCCGGA	1 cut(s)	1289
MroXI	GAANNNNTTC	1 cut(s)	641
MscI	TGGCCA	2 cut(s)	462, 954
MseI	TTAA	5 cut(s)	264, 309, 390, 629, 882
MslI	CAYNNNNRTG	4 cut(s)	468, 597, 948, 954
Msp20I	TGGCCA	2 cut(s)	462, 954
MspCI	CTTAAG	1 cut(s)	881
MspI	CCGG	2 cut(s)	1142, 1290
MspR9I	CCNGG	2 cut(s)	246, 735
MunI	CAATTG	1 cut(s)	394
MvaI	CCWGG	2 cut(s)	246, 735
MwoI	GCNNNNNNNGC	4 cut(s)	377, 380, 833, 1130
NcoI	CCATGG	4 cut(s)	553, 592, 949, 971
NdeII	GATC	4 cut(s)	220, 1024, 1231, 1292
NlaIV	GGNNCC	3 cut(s)	867, 1069, 1167
NmuCI	GTSAC	4 cut(s)	196, 569, 931, 1028
PagI	TCATGA	1 cut(s)	1057
PdmI	GAANNNNTTC	1 cut(s)	641
PfeI	GAWTC	1 cut(s)	313
PflMI	CCANNNNNTGG	3 cut(s)	441, 554, 1201
PfoI	TCCNGGA	1 cut(s)	733
PkrI	GCNGC	6 cut(s)	253, 286, 373, 382, 767, 1135
PleI	GAGTC	4 cut(s)	56, 193, 622, 1214
PpsI	GAGTC	4 cut(s)	56, 193, 622, 1214
PsiI	TTATAA	1 cut(s)	756
Psp6I	CCWGG	2 cut(s)	244, 733
PspGI	CCWGG	2 cut(s)	244, 733
PspN4I	GGNNCC	3 cut(s)	867, 1069, 1167
PspPI	GGNCC	2 cut(s)	866, 1181
PsuI	RGATCY	1 cut(s)	1292
RsaI	GTAC	2 cut(s)	1069, 1157
RsaNI	GTAC	2 cut(s)	1068, 1156
RseI	CAYNNNNRTG	4 cut(s)	468, 597, 948, 954
SaqAI	TTAA	5 cut(s)	264, 309, 390, 629, 882
SatI	GCNGC	6 cut(s)	252, 285, 372, 381, 766, 1134
Sau3AI	GATC	4 cut(s)	220, 1024, 1231, 1292
Sau96I	GGNCC	2 cut(s)	866, 1181
ScaI	AGTACT	1 cut(s)	1157
SchI	GAGTC	4 cut(s)	57, 193, 623, 1215
ScrFI	CCNGG	2 cut(s)	246, 735
SduI	GDGCHC	3 cut(s)	184, 434, 448
SfaNI	GCATC	1 cut(s)	631
SfcI	CTRYAG	1 cut(s)	1159
SinI	GGWCC	1 cut(s)	866
SmiMI	CAYNNNNRTG	4 cut(s)	468, 597, 948, 954
SmlI	CTYRAG	2 cut(s)	32, 881
SmoI	CTYRAG	2 cut(s)	32, 881
Sse9I	AATT	4 cut(s)	265, 288, 394, 894
SsiI	CCGC	2 cut(s)	371, 380
SspMI	CTAG	1 cut(s)	45
StyD4I	CCNGG	2 cut(s)	244, 733
StyI	CCWWGG	6 cut(s)	118, 317, 553, 592, 949, 971
TaaI	ACNGT	1 cut(s)	1041
TaqI	TCGA	1 cut(s)	603
TasI	AATT	4 cut(s)	265, 288, 394, 894
TatI	WGTACW	1 cut(s)	1155
TauI	GCSGC	2 cut(s)	374, 383
TfiI	GAWTC	1 cut(s)	313
Tru1I	TTAA	5 cut(s)	264, 309, 390, 629, 882
Tru9I	TTAA	5 cut(s)	264, 309, 390, 629, 882
TseFI	GTSAC	4 cut(s)	196, 569, 931, 1028
TseI	GCWGC	4 cut(s)	251, 284, 765, 1133
Tsp45I	GTSAC	4 cut(s)	196, 569, 931, 1028
TspDTI	ATGAA	3 cut(s)	518, 972, 1043
TspGWI	ACGGA	2 cut(s)	249, 615
Van91I	CCANNNNNTGG	3 cut(s)	441, 554, 1201
Vha464I	CTTAAG	1 cut(s)	881
VpaK11BI	GGWCC	1 cut(s)	866
XapI	RAATTY	1 cut(s)	894
XcmI	CCANNNNNNNNNTGG	2 cut(s)	300, 555
XmiI	GTMKAC	1 cut(s)	51
XmnI	GAANNNNTTC	1 cut(s)	641
XspI	CTAG	1 cut(s)	45
ZrmI	AGTACT	1 cut(s)	1157

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.