Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0002181

Physical Location & Seq

Genomic Coordinates Reverse (-)

26229625 .. 26232529

Gene Structure 2905 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1154100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1329 bp

ATGGCGACTTGGGTCCTCTCAGAATGCACCCTAAAACCTCTTCCTAAATTCTATCCACATCCCAGAAACGCCCACCCGTCAAAACCCACCGTTCCTTCCTCCCTCCGGCCGTTTCCCACCTCCAAAAGCACCAATCCTTTTGGGTTCAGAGAGGCAAAATGGTCACTCAATGTGAGTGCCCCATTGAGGGTCCCATCCGTGGACGGGGAAGATAATGGGAGAGTGAGCACACATGGGGTTCGAGACGGTGGTGGATTCGACCCCGGTGCCCCTCCCCCCTTCAATTTGGGTGACATCAGGACTGCCATTCCAAAGCATTGCTGGGTTAAGGACCCCTGGAGATCTATGAGCTATGTGGTCAGAGATGTGGCGGTGGTTTTTGGGATGGCGGCTGTTGCAGCCTACTTTAACAATTGGGTTGTTTGGCCTCTCTACTGGGTTGCTCAGGGCACCATGTTCTGGGCTCTCTTTGTTCTTGGACATGATTGTGGCCACGGAAGCTTCTCGAATAATCCGAAACTGAATAGCGTGGTTGGTCATCTTCTTCATTCTTCAATCCTTGTCCCATACCATGGATGGAGAATTAGCCACAGAACTCATCATCAGAACCATGGACACGTCGAAAATGACGAGTCTTGGCATCCGTTGCCTGAGAAAATTTACAAGAGTTTGGATAAGATGACTCAGATATTTAGATTCACCTTGCCATTCCCCATGCTTGCTTATCCTTTCTATCTGTGGAATCGAAGTCCGGGAAAGAATGGCTCTCACTACAATCCAAACAGCGAACTGTTTCTCCCTAATGAAGGGAAAGATATCATTACTTCCACTGCCTGTTGGACAGCAATGGCTGCGCTGCTAGTCGGTCTGTCTTTCACAATGGGTCCTGTCCAAGTGCTTAAGCTCTACGGTGTTCCATACTGGATGTTTGTCTTGTGGTTGGACTTAGTGACTTACTTGCATCACCACGGCCACGAGGACAAGCTTCCATGGTACCGTGGGAAGGAATGGAGTTACCTAAGAGGAGGATTGACAACCATCGATCGCGATTACGGTTGGATCAACAACATCCATCATGACATCGGGACTCATGTTATACATCATCTCTTCCCTCAAATCCCACATTACAACTTAATAGAAGCAACGGAGGCGGCTAAGCCCGTGCTTGGGAAGTACTACAGAGAACCGAAGAAATCGGGGCCTCTCCCGCTTCACTTACTGGGAGTCCTTGTAAGAAGCTTTAAACAAGATCATTACGTGAGTGATACAGGGGATGTTGTATATTATCAAACCGATAAGGAACTCGCCGGATCAGCAGTCCCAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006950 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009536 GO:0009628 GO:0009941 GO:0009987 GO:0016020 GO:0016053 GO:0016491 GO:0016705 GO:0019752 GO:0031090 GO:0031967 GO:0031975 GO:0032787 GO:0042170 GO:0042389 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0055114 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3474 FA_desaturase

Protein Analysis

443

Amino Acids

50.25

Weight (kDa)

8.79

Isoelectric Point (pI)

39.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF3474	PF11960	1 - 131	2.5e-48	Domain of unknown function (DUF3474)
FA_desaturase	PF00487	138 - 393	7.1e-32	Fatty acid desaturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002181)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11170 AT5G05580 AT5G05580
fragaria_vesca	FvH4_6g11310 FvH4_6g11310 FvH4_6g11310 FvH4_6g11310 FvH4_7g24590 FvH4_7g24590 FvH4_7g24590
malus_domestica	MD01G1154100.v1.1 MD04G1155400.v1.1 MD07G1223100.v1.1 MD12G1168600.v1.1 MD17G1258700.v1.1
prunus_persica	Prupe.2G251200_v2.0.a1 Prupe.6G278800_v2.0.a1 Prupe.6G278800_v2.0.a1
pyrus_communis	pycom01g17140 pycom04g13980 pycom07g20170 pycom12g16010
rosa_chinensis	RchiOBHm_Chr1g0370421 RchiOBHm_Chr3g0462851
rosa_laevigata	RLG00000024827 RLG00000027049
rosa_multiflora	Rmu_sc0000766.1_g000013 Rmu_sc0005656.1_g000006
rosa_roxburghii	Rroxscaffold_4G00286590 Rroxscaffold_6G00417140
rosa_rugosa	Rorug01G0356300 Rorug03G0059600
rosa_samantha	Rh1AG364800 Rh1BG328000 Rh1CG341000 Rh1DG358300 Rh3AG117800 Rh3BG121600 Rh3CG124500 Rh3DG123200
rosa_wichuraiana	Rw1G032100 Rw1G032150 Rw3G009990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	993
AccB1I	GGYRCC	3 cut(s)	266, 449, 993
AccB7I	CCANNNNNTGG	2 cut(s)	459, 572
AccII	CGCG	1 cut(s)	1047
AciI	CCGC	4 cut(s)	371, 389, 1151, 1208
AclWI	GGATC	2 cut(s)	1067, 1318
AcoI	YGGCCR	3 cut(s)	107, 490, 970
AcsI	RAATTY	2 cut(s)	47, 657
AfaI	GTAC	2 cut(s)	995, 1175
AflII	CTTAAG	1 cut(s)	899
AflIII	ACRYGT	1 cut(s)	616
AgsI	TTSAA	2 cut(s)	283, 555
AjiI	CACGTC	1 cut(s)	619
AjnI	CCWGG	1 cut(s)	335
AloI	GAACNNNNNNTCC	2 cut(s)	780, 812
AluBI	AGCT	5 cut(s)	351, 501, 904, 985, 1239
AluI	AGCT	5 cut(s)	351, 501, 904, 985, 1239
Alw21I	GWGCWC	1 cut(s)	230
Alw26I	GTCTC	1 cut(s)	237
AlwI	GGATC	2 cut(s)	1067, 1318
AoxI	GGCC	5 cut(s)	107, 425, 490, 970, 1199
ApeKI	GCWGC	3 cut(s)	398, 851, 856
ApoI	RAATTY	2 cut(s)	47, 657
Asp700I	GAANNNNTTC	1 cut(s)	792
Asp718I	GGTACC	1 cut(s)	993
AspLEI	GCGC	1 cut(s)	856
AspS9I	GGNCC	5 cut(s)	13, 190, 331, 884, 1199
AsuC2I	CCSGG	2 cut(s)	264, 753
AsuHPI	GGTGA	3 cut(s)	302, 691, 956
AvaII	GGWCC	4 cut(s)	13, 190, 331, 884
BaeGI	GKGCMC	3 cut(s)	181, 271, 452
BalI	TGGCCA	1 cut(s)	492
BanI	GGYRCC	3 cut(s)	266, 449, 993
BanII	GRGCYC	1 cut(s)	466
BauI	CACGAG	1 cut(s)	974
Bbv12I	GWGCWC	1 cut(s)	230
BbvI	GCAGC	3 cut(s)	410, 838, 843
BccI	CCATC	5 cut(s)	202, 379, 570, 1046, 1080
BceAI	ACGGC	2 cut(s)	94, 985
BcgI	CGANNNNNNTGC	2 cut(s)	248, 282
BciT130I	CCWGG	1 cut(s)	337
BcnI	CCSGG	2 cut(s)	264, 753
BcoDI	GTCTC	1 cut(s)	237
BfaI	CTAG	1 cut(s)	860
BfmI	CTRYAG	1 cut(s)	1177
BfrI	CTTAAG	1 cut(s)	899
BglII	AGATCT	1 cut(s)	341
BisI	GCNGC	5 cut(s)	390, 399, 852, 857, 1152
BlpI	GCTNAGC	1 cut(s)	1155
BlsI	GCNGC	5 cut(s)	391, 400, 853, 858, 1153
BmcAI	AGTACT	1 cut(s)	1175
Bme1390I	CCNGG	3 cut(s)	264, 337, 753
Bme18I	GGWCC	4 cut(s)	13, 190, 331, 884
BmgBI	CACGTC	1 cut(s)	619
BmgT120I	GGNCC	5 cut(s)	13, 190, 331, 884, 1199
BmiI	GGNNCC	9 cut(s)	14, 191, 192, 268, 333, 451, 885, 995, 1200
BmrFI	CCNGG	3 cut(s)	264, 337, 753
BmrI	ACTGGG	2 cut(s)	445, 1229
BmsI	GCATC	2 cut(s)	649, 970
BmuI	ACTGGG	2 cut(s)	445, 1229
BoxI	GACNNNNGTC	1 cut(s)	11
BpmI	CTGGAG	1 cut(s)	358
Bpu10I	CCTNAGC	1 cut(s)	444
Bpu1102I	GCTNAGC	1 cut(s)	1155
BpuMI	CCSGG	2 cut(s)	264, 753
Bsa29I	ATCGAT	1 cut(s)	1041
BsaAI	YACGTR	1 cut(s)	1258
BsaJI	CCNNGG	9 cut(s)	198, 262, 335, 493, 571, 610, 967, 989, 997
BsaXI	ACNNNNNCTCC	4 cut(s)	780, 810, 1215, 1245
Bse1I	ACTGG	3 cut(s)	440, 926, 1224
Bse3DI	GCAATG	2 cut(s)	316, 852
BseBI	CCWGG	1 cut(s)	337
BseCI	ATCGAT	1 cut(s)	1041
BseDI	CCNNGG	9 cut(s)	198, 262, 335, 493, 571, 610, 967, 989, 997
BseGI	GGATG	8 cut(s)	58, 194, 390, 581, 640, 930, 1068, 1279
BseMI	GCAATG	2 cut(s)	316, 852
BseMII	CTCAG	4 cut(s)	33, 458, 642, 698
BseNI	ACTGG	3 cut(s)	440, 926, 1224
BseRI	GAGGAG	1 cut(s)	1038
BseSI	GKGCMC	3 cut(s)	181, 271, 452
BseX3I	CGGCCG	1 cut(s)	107
BseXI	GCAGC	3 cut(s)	410, 838, 843
BseYI	CCCAGC	1 cut(s)	321
Bsh1236I	CGCG	1 cut(s)	1047
Bsh1285I	CGRYCG	2 cut(s)	110, 1045
BshFI	GGCC	5 cut(s)	109, 427, 492, 972, 1201
BshNI	GGYRCC	3 cut(s)	266, 449, 993
BshVI	ATCGAT	1 cut(s)	1041
BsiEI	CGRYCG	2 cut(s)	110, 1045
BsiHKAI	GWGCWC	1 cut(s)	230
BsiSI	CCGG	4 cut(s)	106, 264, 752, 1308
BslFI	GGGAC	4 cut(s)	176, 548, 1099, 1304
BsmAI	GTCTC	1 cut(s)	237
BsmBI	CGTCTC	1 cut(s)	237
BsmFI	GGGAC	4 cut(s)	176, 548, 1099, 1304
BsmI	GAATGC	1 cut(s)	29
BsnI	GGCC	5 cut(s)	109, 427, 492, 972, 1201
Bsp1286I	GDGCHC	5 cut(s)	181, 230, 271, 452, 466
Bsp143I	GATC	5 cut(s)	341, 1042, 1059, 1249, 1310
Bsp1720I	GCTNAGC	1 cut(s)	1155
Bsp19I	CCATGG	3 cut(s)	571, 610, 989
Bsp68I	TCGCGA	1 cut(s)	1047
BspACI	CCGC	4 cut(s)	371, 389, 1151, 1208
BspANI	GGCC	5 cut(s)	109, 427, 492, 972, 1201
BspCNI	CTCAG	4 cut(s)	32, 457, 643, 697
BspDI	ATCGAT	1 cut(s)	1041
BspFNI	CGCG	1 cut(s)	1047
BspHI	TCATGA	1 cut(s)	1075
BspLI	GGNNCC	9 cut(s)	14, 191, 192, 268, 333, 451, 885, 995, 1200
BspPI	GGATC	2 cut(s)	1067, 1318
BspT107I	GGYRCC	3 cut(s)	266, 449, 993
BspTI	CTTAAG	1 cut(s)	899
BsrDI	GCAATG	2 cut(s)	316, 852
BsrI	ACTGG	3 cut(s)	440, 926, 1224
BssECI	CCNNGG	9 cut(s)	198, 262, 335, 493, 571, 610, 967, 989, 997
BssMI	GATC	5 cut(s)	341, 1042, 1059, 1249, 1310
BssSI	CACGAG	1 cut(s)	974
BssT1I	CCWWGG	3 cut(s)	571, 610, 989
Bst2BI	CACGAG	1 cut(s)	974
Bst2UI	CCWGG	1 cut(s)	337
Bst4CI	ACNGT	6 cut(s)	91, 248, 792, 911, 998, 1055
Bst6I	CTCTTC	2 cut(s)	45, 1112
BstAFI	CTTAAG	1 cut(s)	899
BstAPI	GCANNNNNTGC	2 cut(s)	646, 851
BstBAI	YACGTR	1 cut(s)	1258
BstC8I	GCNNGC	1 cut(s)	720
BstDEI	CTNAG	7 cut(s)	19, 444, 651, 684, 946, 1019, 1155
BstDSI	CCRYGG	7 cut(s)	198, 493, 571, 610, 967, 989, 997
BstENI	CCTNNNNNAGG	1 cut(s)	804
BstF5I	GGATG	8 cut(s)	58, 194, 390, 581, 640, 930, 1068, 1279
BstFNI	CGCG	1 cut(s)	1047
BstHHI	GCGC	1 cut(s)	856
BstKTI	GATC	5 cut(s)	344, 1045, 1062, 1252, 1313
BstMAI	GTCTC	1 cut(s)	237
BstMBI	GATC	5 cut(s)	341, 1042, 1059, 1249, 1310
BstMCI	CGRYCG	2 cut(s)	110, 1045
BstMWI	GCNNNNNNNGC	8 cut(s)	395, 398, 498, 646, 851, 1148, 1207, 1313
BstNI	CCWGG	1 cut(s)	337
BstPAI	GACNNNNGTC	1 cut(s)	11
BstSCI	CCNGG	3 cut(s)	262, 335, 751
BstSFI	CTRYAG	1 cut(s)	1177
BstSLI	GKGCMC	3 cut(s)	181, 271, 452
BstUI	CGCG	1 cut(s)	1047
BstV1I	GCAGC	3 cut(s)	410, 838, 843
BstX2I	RGATCY	1 cut(s)	341
BstYI	RGATCY	1 cut(s)	341
BstZI	CGGCCG	1 cut(s)	107
Bsu15I	ATCGAT	1 cut(s)	1041
BsuRI	GGCC	5 cut(s)	109, 427, 492, 972, 1201
BsuTUI	ATCGAT	1 cut(s)	1041
BtgI	CCRYGG	7 cut(s)	198, 493, 571, 610, 967, 989, 997
BtrI	CACGTC	1 cut(s)	619
BtsCI	GGATG	8 cut(s)	58, 194, 390, 581, 640, 930, 1068, 1279
BtsI	GCAGTG	1 cut(s)	828
BtsIMutI	CAGTG	1 cut(s)	828
BtuMI	TCGCGA	1 cut(s)	1047
Cac8I	GCNNGC	1 cut(s)	720
CciI	TCATGA	1 cut(s)	1075
CfoI	GCGC	1 cut(s)	856
Cfr13I	GGNCC	5 cut(s)	13, 190, 331, 884, 1199
ClaI	ATCGAT	1 cut(s)	1041
Csp6I	GTAC	2 cut(s)	994, 1174
CviAII	CATG	9 cut(s)	233, 454, 482, 572, 611, 715, 990, 1076, 1091
CviQI	GTAC	2 cut(s)	994, 1174
DdeI	CTNAG	7 cut(s)	19, 444, 651, 684, 946, 1019, 1155
DpnI	GATC	5 cut(s)	343, 1044, 1061, 1251, 1312
DpnII	GATC	5 cut(s)	341, 1042, 1059, 1249, 1310
DraI	TTTAAA	1 cut(s)	1243
EaeI	YGGCCR	3 cut(s)	107, 490, 970
EagI	CGGCCG	1 cut(s)	107
Eam1104I	CTCTTC	2 cut(s)	45, 1112
EarI	CTCTTC	2 cut(s)	45, 1112
EclXI	CGGCCG	1 cut(s)	107
Eco130I	CCWWGG	3 cut(s)	571, 610, 989
Eco24I	GRGCYC	1 cut(s)	466
Eco32I	GATATC	1 cut(s)	817
Eco47I	GGWCC	4 cut(s)	13, 190, 331, 884
Eco52I	CGGCCG	1 cut(s)	107
EcoNI	CCTNNNNNAGG	1 cut(s)	804
EcoO109I	RGGNCCY	5 cut(s)	13, 190, 331, 884, 1199
EcoRII	CCWGG	1 cut(s)	335
EcoRV	GATATC	1 cut(s)	817
EcoT14I	CCWWGG	3 cut(s)	571, 610, 989
EcoT38I	GRGCYC	1 cut(s)	466
ErhI	CCWWGG	3 cut(s)	571, 610, 989
Esp3I	CGTCTC	1 cut(s)	237
FaeI	CATG	9 cut(s)	236, 457, 485, 575, 614, 718, 993, 1079, 1094
FaqI	GGGAC	4 cut(s)	176, 548, 1099, 1304
FatI	CATG	9 cut(s)	232, 453, 481, 571, 610, 714, 989, 1075, 1090
FauI	CCCGC	1 cut(s)	1215
Fnu4HI	GCNGC	5 cut(s)	390, 399, 852, 857, 1152
FokI	GGATG	8 cut(s)	45, 181, 397, 588, 627, 937, 1055, 1286
FriOI	GRGCYC	1 cut(s)	466
Fsp4HI	GCNGC	5 cut(s)	390, 399, 852, 857, 1152
FspBI	CTAG	1 cut(s)	860
GlaI	GCGC	1 cut(s)	855
GluI	GCNGC	5 cut(s)	390, 399, 852, 857, 1152
GsaI	CCCAGC	1 cut(s)	325
GsuI	CTGGAG	1 cut(s)	358
HaeIII	GGCC	5 cut(s)	109, 427, 492, 972, 1201
HapII	CCGG	4 cut(s)	106, 264, 752, 1308
HhaI	GCGC	1 cut(s)	856
Hin1II	CATG	9 cut(s)	236, 457, 485, 575, 614, 718, 993, 1079, 1094
Hin6I	GCGC	1 cut(s)	854
HinP1I	GCGC	1 cut(s)	854
HindIII	AAGCTT	3 cut(s)	499, 983, 1237
HinfI	GANTC	7 cut(s)	255, 632, 682, 696, 742, 1087, 1224
HpaII	CCGG	4 cut(s)	106, 264, 752, 1308
HphI	GGTGA	3 cut(s)	302, 691, 956
Hpy166II	GTNNAC	1 cut(s)	202
Hpy188I	TCNGA	6 cut(s)	22, 149, 362, 516, 606, 687
Hpy188III	TCNNGA	6 cut(s)	242, 298, 505, 1046, 1076, 1084
Hpy8I	GTNNAC	1 cut(s)	202
Hpy99I	CGWCG	1 cut(s)	623
HpyAV	CCTTC	4 cut(s)	105, 289, 800, 997
HpyCH4III	ACNGT	6 cut(s)	91, 248, 792, 911, 998, 1055
HpyCH4IV	ACGT	2 cut(s)	618, 1257
HpyCH4V	TGCA	3 cut(s)	27, 398, 961
HpyF10VI	GCNNNNNNNGC	8 cut(s)	395, 398, 498, 646, 851, 1148, 1207, 1313
HpyF3I	CTNAG	7 cut(s)	19, 444, 651, 684, 946, 1019, 1155
HpySE526I	ACGT	2 cut(s)	618, 1257
Hsp92II	CATG	9 cut(s)	236, 457, 485, 575, 614, 718, 993, 1079, 1094
HspAI	GCGC	1 cut(s)	854
KflI	GGGWCCC	1 cut(s)	190
KpnI	GGTACC	1 cut(s)	997
Kzo9I	GATC	5 cut(s)	341, 1042, 1059, 1249, 1310
Lsp1109I	GCAGC	3 cut(s)	410, 838, 843
LweI	GCATC	2 cut(s)	649, 970
MaeI	CTAG	1 cut(s)	860
MaeII	ACGT	2 cut(s)	618, 1257
MaeIII	GTNAC	4 cut(s)	162, 290, 949, 1013
MalI	GATC	5 cut(s)	343, 1044, 1061, 1251, 1312
MboI	GATC	5 cut(s)	341, 1042, 1059, 1249, 1310
MboII	GAAGA	7 cut(s)	32, 221, 533, 536, 543, 1099, 1201
MfeI	CAATTG	1 cut(s)	412
MflI	RGATCY	1 cut(s)	341
MhlI	GDGCHC	5 cut(s)	181, 230, 271, 452, 466
MlsI	TGGCCA	1 cut(s)	492
MluCI	AATT	5 cut(s)	47, 283, 412, 582, 657
MluNI	TGGCCA	1 cut(s)	492
MlyI	GAGTC	4 cut(s)	641, 676, 1081, 1233
MmeI	TCCRAC	3 cut(s)	818, 921, 1037
Mox20I	TGGCCA	1 cut(s)	492
MroXI	GAANNNNTTC	1 cut(s)	792
MscI	TGGCCA	1 cut(s)	492
MseI	TTAA	5 cut(s)	327, 408, 900, 1133, 1242
MslI	CAYNNNNRTG	1 cut(s)	486
Msp20I	TGGCCA	1 cut(s)	492
MspCI	CTTAAG	1 cut(s)	899
MspI	CCGG	4 cut(s)	106, 264, 752, 1308
MspR9I	CCNGG	3 cut(s)	264, 337, 753
MunI	CAATTG	1 cut(s)	412
Mva1269I	GAATGC	1 cut(s)	29
MvaI	CCWGG	1 cut(s)	337
MvnI	CGCG	1 cut(s)	1047
MwoI	GCNNNNNNNGC	8 cut(s)	395, 398, 498, 646, 851, 1148, 1207, 1313
NciI	CCSGG	2 cut(s)	264, 753
NcoI	CCATGG	3 cut(s)	571, 610, 989
NdeII	GATC	5 cut(s)	341, 1042, 1059, 1249, 1310
NlaIII	CATG	9 cut(s)	236, 457, 485, 575, 614, 718, 993, 1079, 1094
NlaIV	GGNNCC	9 cut(s)	14, 191, 192, 268, 333, 451, 885, 995, 1200
NmuCI	GTSAC	3 cut(s)	162, 290, 949
NruI	TCGCGA	1 cut(s)	1047
PagI	TCATGA	1 cut(s)	1075
PcsI	WCGNNNNNNNCGW	2 cut(s)	512, 627
PctI	GAATGC	1 cut(s)	29
PdmI	GAANNNNTTC	1 cut(s)	792
PfeI	GAWTC	3 cut(s)	255, 696, 742
PflMI	CCANNNNNTGG	2 cut(s)	459, 572
PfoI	TCCNGGA	1 cut(s)	751
PkrI	GCNGC	5 cut(s)	391, 400, 853, 858, 1153
Ple19I	CGATCG	1 cut(s)	1045
PleI	GAGTC	4 cut(s)	640, 676, 1081, 1232
PpsI	GAGTC	4 cut(s)	640, 676, 1081, 1232
Ppu21I	YACGTR	1 cut(s)	1258
PpuMI	RGGWCCY	4 cut(s)	13, 190, 331, 884
PshAI	GACNNNNGTC	1 cut(s)	11
Psp5II	RGGWCCY	4 cut(s)	13, 190, 331, 884
Psp6I	CCWGG	1 cut(s)	335
PspFI	CCCAGC	1 cut(s)	321
PspGI	CCWGG	1 cut(s)	335
PspN4I	GGNNCC	9 cut(s)	14, 191, 192, 268, 333, 451, 885, 995, 1200
PspPI	GGNCC	5 cut(s)	13, 190, 331, 884, 1199
PspPPI	RGGWCCY	4 cut(s)	13, 190, 331, 884
PsuI	RGATCY	1 cut(s)	341
PvuI	CGATCG	1 cut(s)	1045
RruI	TCGCGA	1 cut(s)	1047
RsaI	GTAC	2 cut(s)	995, 1175
RsaNI	GTAC	2 cut(s)	994, 1174
RseI	CAYNNNNRTG	1 cut(s)	486
SaqAI	TTAA	5 cut(s)	327, 408, 900, 1133, 1242
SatI	GCNGC	5 cut(s)	390, 399, 852, 857, 1152
Sau3AI	GATC	5 cut(s)	341, 1042, 1059, 1249, 1310
Sau96I	GGNCC	5 cut(s)	13, 190, 331, 884, 1199
ScaI	AGTACT	1 cut(s)	1175
SchI	GAGTC	4 cut(s)	641, 676, 1081, 1233
ScrFI	CCNGG	3 cut(s)	264, 337, 753
SduI	GDGCHC	5 cut(s)	181, 230, 271, 452, 466
SfaNI	GCATC	2 cut(s)	649, 970
SfcI	CTRYAG	1 cut(s)	1177
SinI	GGWCC	4 cut(s)	13, 190, 331, 884
SmiMI	CAYNNNNRTG	1 cut(s)	486
SmlI	CTYRAG	1 cut(s)	899
SmoI	CTYRAG	1 cut(s)	899
Sse9I	AATT	5 cut(s)	47, 283, 412, 582, 657
SsiI	CCGC	4 cut(s)	371, 389, 1151, 1208
SspMI	CTAG	1 cut(s)	860
StyD4I	CCNGG	3 cut(s)	262, 335, 751
StyI	CCWWGG	3 cut(s)	571, 610, 989
TaaI	ACNGT	6 cut(s)	91, 248, 792, 911, 998, 1055
TaiI	ACGT	2 cut(s)	621, 1260
TaqI	TCGA	6 cut(s)	241, 258, 506, 621, 745, 1041
TaqII	GACCGA	1 cut(s)	854
TasI	AATT	5 cut(s)	47, 283, 412, 582, 657
TatI	WGTACW	1 cut(s)	1173
TauI	GCSGC	2 cut(s)	392, 1154
TfiI	GAWTC	3 cut(s)	255, 696, 742
Tru1I	TTAA	5 cut(s)	327, 408, 900, 1133, 1242
Tru9I	TTAA	5 cut(s)	327, 408, 900, 1133, 1242
TscAI	CASTG	1 cut(s)	835
TseFI	GTSAC	3 cut(s)	162, 290, 949
TseI	GCWGC	3 cut(s)	398, 851, 856
Tsp45I	GTSAC	3 cut(s)	162, 290, 949
TspDTI	ATGAA	2 cut(s)	536, 819
TspGWI	ACGGA	4 cut(s)	187, 510, 633, 1160
TspRI	CASTG	1 cut(s)	835
Van91I	CCANNNNNTGG	2 cut(s)	459, 572
Vha464I	CTTAAG	1 cut(s)	899
VpaK11BI	GGWCC	4 cut(s)	13, 190, 331, 884
XagI	CCTNNNNNAGG	1 cut(s)	804
XapI	RAATTY	2 cut(s)	47, 657
XcmI	CCANNNNNNNNNTGG	2 cut(s)	318, 573
XmnI	GAANNNNTTC	1 cut(s)	792
XspI	CTAG	1 cut(s)	860
ZrmI	AGTACT	1 cut(s)	1175

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.