Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002181

Physical Location & Seq

Genomic Coordinates Reverse (-)

24900958 .. 24903916

Gene Structure 2959 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1168600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1362 bp

ATGGCGAGTTGGGTCCTCTCTGAATGCGGCCTAAAGCCTCTCCCGCAAGTTTGGAACCGACCCAGAACCGCAGTTTGCTCTAGCTACCGGTCAAAAGTTCGGATTTTTCGGTCAAACCAGAGCTCGGCGGATCTGAGATTGGATTCGACCAAAATCGCAGGTGGGTTGTTTAGAGAGAGAAGATGGGATCTTAACGTGAGCGCTCCGGTGAGGGTAGCTTCTGTTGAAGAGGAGGAGGAGAGAGTTAACGGCGTTAATGGCGGCGGAGATGGAGAAGAGGAGGTGGGATTTGATCCGGGTGCGCCGCCGCCGTTCAAATTAGCCGATGTTAAAGCTGCCATTCCGAAGCATTGTTGGGTTAGGGACCCGTGGAGGTCAATGAGCTATGTGGTGAGGGATGTGGCGGTGGTTTTCGGGTTGGCTGCGGCTGCGGTTTATGTGAACAAGTGGTTTGTTTGGCCTCTGTATTGGGCTGCTCAGGGGACAATGTTTTGGGCTATCTTTGTTCTTGGCCATGATTGTGGCCATGGAAGCTTTTCGAATAATCCCAAGCTAAACAGTGTTGTGGGGCATCTCTTGCATTCTTCAATTCTTGTACCCTATCATGGATGGAGAATTAGCCACAGGACTCATCATCAAAACCATGGTCATGTTGAGAATGACGAGTCATGGCACCCGTTGTCTGAAAAAATCTACAAGAAATTGGATAGCATGACACGAATTTTGCGCTTCACCGTTCCTTTTCCCATGCTTGCTTATCCTTTCTATCTTTGGAATAGAAGTCCAGGAAAGACTGGTTCCCACTTTGACCCGAAGAGCGATCTATTTGTCCCGAATGAGAGGAAAGATGTGATCACTTCCACTGTATGTTGGACAGCAATGGCTGCTTTACTTGTGGGATTATCGTTTGTAATGGGTCCTGTTCAAGTGCTTAAGCTCTACGGCATTCCCTACTGGGTTTTTGTCATGTGGCTGGATTTGGTAACTTACTTGCATCACCATGGGCACGAAGACAAACTACCATGGTATCGAGGGAAGGAATGGAGTTATCTGAGGGGAGGGCTTACGACACTGGATCGGGACTACGGATTGATCAATAACATCCACCACGACATCGGAACCCATGTGGTTCATCATCTTTTTCCTCAAATCCCCCACTACCACTTGGTTGAAGCAACTGAGGCAGCAAAGCCGGTATTCGGGAACTACTACAGAGAGCCGAAGAAATCAGGACCTCTACCATTTCACTTGCTTGGAAGTTTTATCAAAAGCTTGAAAAAGGACCACTATGTGAGTGACAATGGCGATGTTGTGTACTACCAAACTGACCCTAGCTTTGGTGGCTTTCCTCCATCAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3474 FA_desaturase

Protein Analysis

454

Amino Acids

51.61

Weight (kDa)

8.74

Isoelectric Point (pI)

39.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF3474	PF11960	1 - 142	3.6e-58	Domain of unknown function (DUF3474)
FA_desaturase	PF00487	150 - 404	1.3e-31	Fatty acid desaturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002181)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11170 AT5G05580 AT5G05580
fragaria_vesca	FvH4_6g11310 FvH4_6g11310 FvH4_6g11310 FvH4_6g11310 FvH4_7g24590 FvH4_7g24590 FvH4_7g24590
malus_domestica	MD01G1154100.v1.1 MD04G1155400.v1.1 MD07G1223100.v1.1 MD12G1168600.v1.1 MD17G1258700.v1.1
prunus_persica	Prupe.2G251200_v2.0.a1 Prupe.6G278800_v2.0.a1 Prupe.6G278800_v2.0.a1
pyrus_communis	pycom01g17140 pycom04g13980 pycom07g20170 pycom12g16010
rosa_chinensis	RchiOBHm_Chr1g0370421 RchiOBHm_Chr3g0462851
rosa_laevigata	RLG00000024827 RLG00000027049
rosa_multiflora	Rmu_sc0000766.1_g000013 Rmu_sc0005656.1_g000006
rosa_roxburghii	Rroxscaffold_4G00286590 Rroxscaffold_6G00417140
rosa_rugosa	Rorug01G0356300 Rorug03G0059600
rosa_samantha	Rh1AG364800 Rh1BG328000 Rh1CG341000 Rh1DG358300 Rh3AG117800 Rh3BG121600 Rh3CG124500 Rh3DG123200
rosa_wichuraiana	Rw1G032100 Rw1G032150 Rw3G009990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	149
Acc36I	ACCTGC	1 cut(s)	149
AccB1I	GGYRCC	1 cut(s)	672
AclWI	GGATC	4 cut(s)	138, 195, 287, 1083
AcoI	YGGCCR	2 cut(s)	511, 523
AcsI	RAATTY	1 cut(s)	720
AdeI	CACNNNGTG	1 cut(s)	1291
AfaI	GTAC	2 cut(s)	597, 1316
AfeI	AGCGCT	1 cut(s)	202
AfiI	CCNNNNNNNGG	4 cut(s)	124, 605, 1199, 1337
AflII	CTTAAG	1 cut(s)	932
AgeI	ACCGGT	1 cut(s)	87
AgsI	TTSAA	6 cut(s)	227, 316, 588, 926, 1172, 1276
AjnI	CCWGG	1 cut(s)	784
Alw21I	GWGCWC	1 cut(s)	125
AlwI	GGATC	4 cut(s)	138, 195, 287, 1083
Aor51HI	AGCGCT	1 cut(s)	202
AoxI	GGCC	4 cut(s)	28, 458, 511, 523
ApeKI	GCWGC	6 cut(s)	335, 422, 428, 473, 884, 1184
ApoI	RAATTY	1 cut(s)	720
ArsI	GACNNNNNNTTYG	6 cut(s)	631, 663, 706, 738, 1319, 1351
AsiGI	ACCGGT	1 cut(s)	87
Asp700I	GAANNNNTTC	1 cut(s)	535
AspLEI	GCGC	3 cut(s)	203, 304, 729
AspS9I	GGNCC	5 cut(s)	13, 364, 917, 1232, 1282
AsuC2I	CCSGG	1 cut(s)	297
AsuHPI	GGTGA	4 cut(s)	220, 403, 724, 989
AsuII	TTCGAA	1 cut(s)	539
AvaII	GGWCC	5 cut(s)	13, 364, 917, 1232, 1282
BaeGI	GKGCMC	1 cut(s)	1008
BaeI	ACNNNNGTAYC	2 cut(s)	1010, 1043
BalI	TGGCCA	2 cut(s)	513, 525
BanI	GGYRCC	1 cut(s)	672
BanII	GRGCYC	1 cut(s)	125
BarI	GAAGNNNNNNTAC	2 cut(s)	1002, 1034
BbsI	GAAGAC	1 cut(s)	1017
Bbv12I	GWGCWC	1 cut(s)	125
BbvI	GCAGC	6 cut(s)	322, 409, 415, 460, 871, 1196
BccI	CCATC	4 cut(s)	177, 263, 603, 1360
BceAI	ACGGC	3 cut(s)	265, 295, 958
BciT130I	CCWGG	1 cut(s)	786
BclI	TGATCA	2 cut(s)	852, 1092
BcnI	CCSGG	1 cut(s)	297
BfaI	CTAG	2 cut(s)	81, 1332
BfmI	CTRYAG	1 cut(s)	1210
BfoI	RGCGCY	1 cut(s)	204
BfrI	CTTAAG	1 cut(s)	932
BfuAI	ACCTGC	1 cut(s)	149
Bme1390I	CCNGG	2 cut(s)	297, 786
Bme18I	GGWCC	5 cut(s)	13, 364, 917, 1232, 1282
BmgT120I	GGNCC	5 cut(s)	13, 364, 917, 1232, 1282
BmiI	GGNNCC	8 cut(s)	14, 56, 365, 366, 674, 799, 918, 1120
BmrFI	CCNGG	2 cut(s)	297, 786
BmrI	ACTGGG	1 cut(s)	964
BmsI	GCATC	2 cut(s)	580, 1003
BmuI	ACTGGG	1 cut(s)	964
BpiI	GAAGAC	1 cut(s)	1017
Bpu10I	CCTNAGC	1 cut(s)	477
Bpu14I	TTCGAA	1 cut(s)	539
BpuMI	CCSGG	1 cut(s)	297
BsaJI	CCNNGG	5 cut(s)	368, 526, 643, 1000, 1022
BsaWI	WCCGGW	2 cut(s)	87, 205
Bsc4I	CCNNNNNNNGG	4 cut(s)	124, 605, 1199, 1337
Bse118I	RCCGGY	2 cut(s)	87, 1192
Bse1I	ACTGG	3 cut(s)	799, 959, 1077
Bse3DI	GCAATG	1 cut(s)	885
BseBI	CCWGG	1 cut(s)	786
BseDI	CCNNGG	5 cut(s)	368, 526, 643, 1000, 1022
BseGI	GGATG	3 cut(s)	403, 614, 1101
BseLI	CCNNNNNNNGG	4 cut(s)	124, 605, 1199, 1337
BseMI	GCAATG	1 cut(s)	885
BseMII	CTCAG	4 cut(s)	125, 491, 1043, 1170
BseNI	ACTGG	3 cut(s)	799, 959, 1077
BseRI	GAGGAG	4 cut(s)	245, 248, 251, 293
BseSI	GKGCMC	1 cut(s)	1008
BseXI	GCAGC	6 cut(s)	322, 409, 415, 460, 871, 1196
BshFI	GGCC	4 cut(s)	30, 460, 513, 525
BshNI	GGYRCC	1 cut(s)	672
BshTI	ACCGGT	1 cut(s)	87
BsiHKAI	GWGCWC	1 cut(s)	125
BsiSI	CCGG	4 cut(s)	88, 206, 296, 1193
BslFI	GGGAC	4 cut(s)	377, 496, 815, 1094
BslI	CCNNNNNNNGG	4 cut(s)	124, 605, 1199, 1337
BsmFI	GGGAC	4 cut(s)	377, 496, 815, 1094
BsmI	GAATGC	3 cut(s)	29, 580, 945
BsnI	GGCC	4 cut(s)	30, 460, 513, 525
Bsp119I	TTCGAA	1 cut(s)	539
Bsp1286I	GDGCHC	2 cut(s)	125, 1008
Bsp143I	GATC	7 cut(s)	130, 187, 292, 820, 852, 1075, 1092
Bsp19I	CCATGG	4 cut(s)	526, 643, 1000, 1022
BspANI	GGCC	4 cut(s)	30, 460, 513, 525
BspCNI	CTCAG	4 cut(s)	126, 490, 1044, 1171
BspLI	GGNNCC	8 cut(s)	14, 56, 365, 366, 674, 799, 918, 1120
BspMI	ACCTGC	1 cut(s)	149
BspPI	GGATC	4 cut(s)	138, 195, 287, 1083
BspQI	GCTCTTC	1 cut(s)	809
BspT104I	TTCGAA	1 cut(s)	539
BspT107I	GGYRCC	1 cut(s)	672
BspTI	CTTAAG	1 cut(s)	932
BsrDI	GCAATG	1 cut(s)	885
BsrFI	RCCGGY	2 cut(s)	87, 1192
BsrI	ACTGG	3 cut(s)	799, 959, 1077
BssAI	RCCGGY	2 cut(s)	87, 1192
BssECI	CCNNGG	5 cut(s)	368, 526, 643, 1000, 1022
BssMI	GATC	7 cut(s)	130, 187, 292, 820, 852, 1075, 1092
BssT1I	CCWWGG	4 cut(s)	526, 643, 1000, 1022
Bst2UI	CCWGG	1 cut(s)	786
Bst4CI	ACNGT	3 cut(s)	560, 736, 865
Bst6I	CTCTTC	3 cut(s)	222, 270, 809
BstAFI	CTTAAG	1 cut(s)	932
BstAPI	GCANNNNNTGC	2 cut(s)	577, 884
BstBI	TTCGAA	1 cut(s)	539
BstC8I	GCNNGC	1 cut(s)	753
BstDEI	CTNAG	4 cut(s)	134, 477, 1052, 1179
BstDSI	CCRYGG	5 cut(s)	368, 526, 643, 1000, 1022
BstF5I	GGATG	3 cut(s)	403, 614, 1101
BstH2I	RGCGCY	1 cut(s)	204
BstHHI	GCGC	3 cut(s)	203, 304, 729
BstKTI	GATC	7 cut(s)	133, 190, 295, 823, 855, 1078, 1095
BstMBI	GATC	7 cut(s)	130, 187, 292, 820, 852, 1075, 1092
BstMWI	GCNNNNNNNGC	8 cut(s)	43, 258, 428, 531, 577, 884, 1181, 1341
BstNI	CCWGG	1 cut(s)	786
BstSCI	CCNGG	2 cut(s)	295, 784
BstSFI	CTRYAG	1 cut(s)	1210
BstSLI	GKGCMC	1 cut(s)	1008
BstV1I	GCAGC	6 cut(s)	322, 409, 415, 460, 871, 1196
BstV2I	GAAGAC	1 cut(s)	1017
BstX2I	RGATCY	2 cut(s)	130, 187
BstXI	CCANNNNNNTGG	1 cut(s)	521
BstYI	RGATCY	2 cut(s)	130, 187
BsuRI	GGCC	4 cut(s)	30, 460, 513, 525
BtgI	CCRYGG	5 cut(s)	368, 526, 643, 1000, 1022
BtgZI	GCGATG	1 cut(s)	1320
BtsCI	GGATG	3 cut(s)	403, 614, 1101
BtsIMutI	CAGTG	3 cut(s)	565, 861, 1070
BveI	ACCTGC	1 cut(s)	149
Cac8I	GCNNGC	1 cut(s)	753
CfoI	GCGC	3 cut(s)	203, 304, 729
Cfr10I	RCCGGY	2 cut(s)	87, 1192
Cfr13I	GGNCC	5 cut(s)	13, 364, 917, 1232, 1282
Csp6I	GTAC	2 cut(s)	596, 1315
CspAI	ACCGGT	1 cut(s)	87
CviQI	GTAC	2 cut(s)	596, 1315
DdeI	CTNAG	4 cut(s)	134, 477, 1052, 1179
DpnI	GATC	7 cut(s)	132, 189, 294, 822, 854, 1077, 1094
DpnII	GATC	7 cut(s)	130, 187, 292, 820, 852, 1075, 1092
DraIII	CACNNNGTG	1 cut(s)	1291
EaeI	YGGCCR	2 cut(s)	511, 523
Eam1104I	CTCTTC	3 cut(s)	222, 270, 809
EarI	CTCTTC	3 cut(s)	222, 270, 809
EciI	GGCGGA	2 cut(s)	143, 279
Ecl136II	GAGCTC	1 cut(s)	123
Eco130I	CCWWGG	4 cut(s)	526, 643, 1000, 1022
Eco24I	GRGCYC	1 cut(s)	125
Eco47I	GGWCC	5 cut(s)	13, 364, 917, 1232, 1282
Eco47III	AGCGCT	1 cut(s)	202
Eco53kI	GAGCTC	1 cut(s)	123
EcoICRI	GAGCTC	1 cut(s)	123
EcoO109I	RGGNCCY	4 cut(s)	13, 364, 917, 1232
EcoRII	CCWGG	1 cut(s)	784
EcoT14I	CCWWGG	4 cut(s)	526, 643, 1000, 1022
EcoT38I	GRGCYC	1 cut(s)	125
ErhI	CCWWGG	4 cut(s)	526, 643, 1000, 1022
FaqI	GGGAC	4 cut(s)	377, 496, 815, 1094
FauI	CCCGC	1 cut(s)	51
FbaI	TGATCA	2 cut(s)	852, 1092
FokI	GGATG	3 cut(s)	410, 621, 1088
FriOI	GRGCYC	1 cut(s)	125
FspBI	CTAG	2 cut(s)	81, 1332
GlaI	GCGC	3 cut(s)	202, 303, 728
HaeII	RGCGCY	1 cut(s)	204
HaeIII	GGCC	4 cut(s)	30, 460, 513, 525
HapII	CCGG	4 cut(s)	88, 206, 296, 1193
HhaI	GCGC	3 cut(s)	203, 304, 729
Hin6I	GCGC	3 cut(s)	201, 302, 727
HinP1I	GCGC	3 cut(s)	201, 302, 727
HincII	GTYRAC	1 cut(s)	247
HindII	GTYRAC	1 cut(s)	247
HindIII	AAGCTT	2 cut(s)	532, 1270
HinfI	GANTC	3 cut(s)	143, 628, 665
HpaI	GTTAAC	1 cut(s)	247
HpaII	CCGG	4 cut(s)	88, 206, 296, 1193
HphI	GGTGA	4 cut(s)	220, 403, 724, 989
Hpy166II	GTNNAC	3 cut(s)	247, 442, 1315
Hpy188I	TCNGA	7 cut(s)	22, 102, 135, 345, 685, 1053, 1118
Hpy188III	TCNNGA	4 cut(s)	832, 1079, 1201, 1230
Hpy8I	GTNNAC	3 cut(s)	247, 442, 1315
HpyAV	CCTTC	1 cut(s)	1030
HpyCH4III	ACNGT	3 cut(s)	560, 736, 865
HpyCH4IV	ACGT	1 cut(s)	195
HpyCH4V	TGCA	2 cut(s)	580, 994
HpyF10VI	GCNNNNNNNGC	8 cut(s)	43, 258, 428, 531, 577, 884, 1181, 1341
HpyF3I	CTNAG	4 cut(s)	134, 477, 1052, 1179
HpySE526I	ACGT	1 cut(s)	195
HspAI	GCGC	3 cut(s)	201, 302, 727
KflI	GGGWCCC	1 cut(s)	364
Ksp22I	TGATCA	2 cut(s)	852, 1092
KspAI	GTTAAC	1 cut(s)	247
Kzo9I	GATC	7 cut(s)	130, 187, 292, 820, 852, 1075, 1092
LguI	GCTCTTC	1 cut(s)	809
LmnI	GCTCC	1 cut(s)	208
Lsp1109I	GCAGC	6 cut(s)	322, 409, 415, 460, 871, 1196
LweI	GCATC	2 cut(s)	580, 1003
MaeI	CTAG	2 cut(s)	81, 1332
MaeII	ACGT	1 cut(s)	195
MaeIII	GTNAC	2 cut(s)	982, 1295
MalI	GATC	7 cut(s)	132, 189, 294, 822, 854, 1077, 1094
MboI	GATC	7 cut(s)	130, 187, 292, 820, 852, 1075, 1092
MboII	GAAGA	7 cut(s)	192, 239, 287, 576, 826, 1022, 1234
MflI	RGATCY	2 cut(s)	130, 187
MhlI	GDGCHC	2 cut(s)	125, 1008
MlsI	TGGCCA	2 cut(s)	513, 525
MluCI	AATT	6 cut(s)	317, 588, 615, 701, 720, 1357
MluNI	TGGCCA	2 cut(s)	513, 525
MlyI	GAGTC	2 cut(s)	622, 674
MmeI	TCCRAC	1 cut(s)	851
Mox20I	TGGCCA	2 cut(s)	513, 525
MroXI	GAANNNNTTC	1 cut(s)	535
MscI	TGGCCA	2 cut(s)	513, 525
MseI	TTAA	6 cut(s)	192, 246, 255, 330, 933, 1360
MslI	CAYNNNNRTG	3 cut(s)	519, 648, 999
Msp20I	TGGCCA	2 cut(s)	513, 525
MspCI	CTTAAG	1 cut(s)	932
MspI	CCGG	4 cut(s)	88, 206, 296, 1193
MspR9I	CCNGG	2 cut(s)	297, 786
Mva1269I	GAATGC	3 cut(s)	29, 580, 945
MvaI	CCWGG	1 cut(s)	786
MwoI	GCNNNNNNNGC	8 cut(s)	43, 258, 428, 531, 577, 884, 1181, 1341
NciI	CCSGG	1 cut(s)	297
NcoI	CCATGG	4 cut(s)	526, 643, 1000, 1022
NdeII	GATC	7 cut(s)	130, 187, 292, 820, 852, 1075, 1092
NlaIV	GGNNCC	8 cut(s)	14, 56, 365, 366, 674, 799, 918, 1120
NmeAIII	GCCGAG	1 cut(s)	104
NmuCI	GTSAC	1 cut(s)	1295
NspV	TTCGAA	1 cut(s)	539
PaqCI	CACCTGC	1 cut(s)	149
PciSI	GCTCTTC	1 cut(s)	809
PctI	GAATGC	3 cut(s)	29, 580, 945
PdmI	GAANNNNTTC	1 cut(s)	535
PfeI	GAWTC	1 cut(s)	143
PfoI	TCCNGGA	1 cut(s)	784
PinAI	ACCGGT	1 cut(s)	87
PleI	GAGTC	2 cut(s)	622, 673
PpsI	GAGTC	2 cut(s)	622, 673
PpuMI	RGGWCCY	4 cut(s)	13, 364, 917, 1232
Psp124BI	GAGCTC	1 cut(s)	125
Psp5II	RGGWCCY	4 cut(s)	13, 364, 917, 1232
Psp6I	CCWGG	1 cut(s)	784
PspGI	CCWGG	1 cut(s)	784
PspN4I	GGNNCC	8 cut(s)	14, 56, 365, 366, 674, 799, 918, 1120
PspPI	GGNCC	5 cut(s)	13, 364, 917, 1232, 1282
PspPPI	RGGWCCY	4 cut(s)	13, 364, 917, 1232
PsuI	RGATCY	2 cut(s)	130, 187
RsaI	GTAC	2 cut(s)	597, 1316
RsaNI	GTAC	2 cut(s)	596, 1315
RseI	CAYNNNNRTG	3 cut(s)	519, 648, 999
SacI	GAGCTC	1 cut(s)	125
SapI	GCTCTTC	1 cut(s)	809
SaqAI	TTAA	6 cut(s)	192, 246, 255, 330, 933, 1360
Sau3AI	GATC	7 cut(s)	130, 187, 292, 820, 852, 1075, 1092
Sau96I	GGNCC	5 cut(s)	13, 364, 917, 1232, 1282
SchI	GAGTC	2 cut(s)	622, 674
ScrFI	CCNGG	2 cut(s)	297, 786
SduI	GDGCHC	2 cut(s)	125, 1008
SfaNI	GCATC	2 cut(s)	580, 1003
SfcI	CTRYAG	1 cut(s)	1210
SfuI	TTCGAA	1 cut(s)	539
SinI	GGWCC	5 cut(s)	13, 364, 917, 1232, 1282
SmiMI	CAYNNNNRTG	3 cut(s)	519, 648, 999
SmlI	CTYRAG	1 cut(s)	932
SmoI	CTYRAG	1 cut(s)	932
Sse9I	AATT	6 cut(s)	317, 588, 615, 701, 720, 1357
SspMI	CTAG	2 cut(s)	81, 1332
SstI	GAGCTC	1 cut(s)	125
StyD4I	CCNGG	2 cut(s)	295, 784
StyI	CCWWGG	4 cut(s)	526, 643, 1000, 1022
TaaI	ACNGT	3 cut(s)	560, 736, 865
TaiI	ACGT	1 cut(s)	198
TaqI	TCGA	3 cut(s)	146, 539, 1030
TaqII	GACCGA	1 cut(s)	99
TasI	AATT	6 cut(s)	317, 588, 615, 701, 720, 1357
TatI	WGTACW	1 cut(s)	1314
TauI	GCSGC	5 cut(s)	30, 264, 307, 310, 428
TfiI	GAWTC	1 cut(s)	143
Tru1I	TTAA	6 cut(s)	192, 246, 255, 330, 933, 1360
Tru9I	TTAA	6 cut(s)	192, 246, 255, 330, 933, 1360
TscAI	CASTG	3 cut(s)	565, 868, 1077
TseFI	GTSAC	1 cut(s)	1295
TseI	GCWGC	6 cut(s)	335, 422, 428, 473, 884, 1184
Tsp45I	GTSAC	1 cut(s)	1295
TspDTI	ATGAA	1 cut(s)	1121
TspGWI	ACGGA	1 cut(s)	1101
TspRI	CASTG	3 cut(s)	565, 868, 1077
Vha464I	CTTAAG	1 cut(s)	932
VpaK11BI	GGWCC	5 cut(s)	13, 364, 917, 1232, 1282
XapI	RAATTY	1 cut(s)	720
XmnI	GAANNNNTTC	1 cut(s)	535
XspI	CTAG	2 cut(s)	81, 1332

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.