Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0002181

Physical Location & Seq

Genomic Coordinates Reverse (-)

18460445 .. 18462704

Gene Structure 2260 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g16010.5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1362 bp

ATGGCGAGTTGGGTCCTCTCTGAATGCGGCCTAAAGCCTCTCCCGCAAGTTTGGAACCAACCCAGAACCGCAGTTTGCTCTAGCTACCCGTCAAAAGTTCGGATTTTTGGGTCAAACCAGAGCTCGGCGGATCTGAGATTGGATTCGACCAAAATCGGAGGTGGGTTGTTTAGAGAGAGAAGATGGGGTCTAAACGTGAGCGCTCCGGTGAGGGTAGCTGCTGTTGAAGAGGAGGAGGAGAGGGTTAACGGCGTTAATGGCGGCGGAGATGGAGAAGAGGAGGTGGGATTTGATCCCGGTGCGCCGCCGCCGTTCAAATTGGCCGACGTTAAAGCTGCCATTCCGAAGCATTGTTGGGTTAGGGACCCGTGGAGGTCAATGAGCTATGTGGTGAGGGATGTGGCGGTGGTTTTCGGGTTGGCTGCGGCTGCGGTTTATGTGAACAAGTGGTTTGTTTGGCCTCTGTATTGGGCTGCTCAGGGGACAATGTTTTGGGCTCTCTTTGTTCTTGGCCATGATTGTGGCCATGGAAGCTTTTCGAATAATCCCAAGCTAAACAGTGTTGTGGGGCATCTCTTGCATTCTTCAATTCTTGTACCCTATCATGGATGGAGAATTAGCCACAGGACTCATCATCAAAACCACGGTCATGTCGAGAATGACGAGTCATGGCACCCGTTGTCTGAGAAAATCTACAAGAAATTGGATAGCATGACACGAATTTTGCGCTTCACCGTACCTTTTCCCATGCTTGCTTATCCTTTCTATCTTTGGAATAGAAGTCCAGGAAAGACTGGTTCCCACTTTGACCCGAAGAGCGATCTATTTGTCCCGAATGAGAGGAAAGATGTGACCACTTCCACAGTATGTTGGACAGCAATGGCTGGGTTACTTGTGGGATTATCCTTTGTAATGGGTCCTGTTCAAGTGCTTAAGCTCTATGGCATTCCCTACTGGGTTTTTGTCATGTGGCTGGATTTGGTAACTTACTTGCATCACCATGGCCACGAAGACAAATTACCATGGTATCGAGGGAAGGAATGGAGTTATCTGAGGGGAGGGCTTACGACACTGGATCGAGACTACGGATTGATCAATAACATCCACCACGACATCGGAACCCATGTGGTTCATCATCTTTTTCCTCAAATCCCCCACTACCACTTAGTTGAAGCAACTGAGGCAGCAAAGCCGGTATTCGGGAACTACTACAGAGAGCCGAAGAAATCAGGACCTCTACCATTTCACTTGCTTGGAAGTTTTATCAAAAGCTTGAAAAAAGACCACTATGTGAGCGACAATGGCGATGTTGTGTACTACCAAACTGACCCTAGCTTTGGTGGCTTTCCTCCATCAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3474 FA_desaturase

Protein Analysis

454

Amino Acids

51.31

Weight (kDa)

8.42

Isoelectric Point (pI)

38.53

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002181)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11170 AT5G05580 AT5G05580
fragaria_vesca	FvH4_6g11310 FvH4_6g11310 FvH4_6g11310 FvH4_6g11310 FvH4_7g24590 FvH4_7g24590 FvH4_7g24590
malus_domestica	MD01G1154100.v1.1 MD04G1155400.v1.1 MD07G1223100.v1.1 MD12G1168600.v1.1 MD17G1258700.v1.1
prunus_persica	Prupe.2G251200_v2.0.a1 Prupe.6G278800_v2.0.a1 Prupe.6G278800_v2.0.a1
pyrus_communis	pycom01g17140 pycom04g13980 pycom07g20170 pycom12g16010
rosa_chinensis	RchiOBHm_Chr1g0370421 RchiOBHm_Chr3g0462851
rosa_laevigata	RLG00000024827 RLG00000027049
rosa_multiflora	Rmu_sc0000766.1_g000013 Rmu_sc0005656.1_g000006
rosa_roxburghii	Rroxscaffold_4G00286590 Rroxscaffold_6G00417140
rosa_rugosa	Rorug01G0356300 Rorug03G0059600
rosa_samantha	Rh1AG364800 Rh1BG328000 Rh1CG341000 Rh1DG358300 Rh3AG117800 Rh3BG121600 Rh3CG124500 Rh3DG123200
rosa_wichuraiana	Rw1G032100 Rw1G032150 Rw3G009990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	672
AclWI	GGATC	3 cut(s)	138, 287, 1083
AcoI	YGGCCR	4 cut(s)	321, 511, 523, 1003
AcsI	RAATTY	1 cut(s)	720
AdeI	CACNNNGTG	1 cut(s)	1291
AfaI	GTAC	3 cut(s)	597, 738, 1316
AfeI	AGCGCT	1 cut(s)	202
AfiI	CCNNNNNNNGG	4 cut(s)	124, 605, 1199, 1337
AflII	CTTAAG	1 cut(s)	932
AgsI	TTSAA	6 cut(s)	227, 316, 588, 926, 1172, 1276
AjnI	CCWGG	1 cut(s)	784
Alw21I	GWGCWC	1 cut(s)	125
Alw26I	GTCTC	1 cut(s)	1074
AlwI	GGATC	3 cut(s)	138, 287, 1083
Aor51HI	AGCGCT	1 cut(s)	202
AoxI	GGCC	6 cut(s)	28, 321, 458, 511, 523, 1003
ApeKI	GCWGC	6 cut(s)	218, 335, 422, 428, 473, 1184
ApoI	RAATTY	1 cut(s)	720
ArsI	GACNNNNNNTTYG	6 cut(s)	631, 663, 706, 738, 1319, 1351
Asp700I	GAANNNNTTC	1 cut(s)	535
AspLEI	GCGC	3 cut(s)	203, 304, 729
AspS9I	GGNCC	4 cut(s)	13, 364, 917, 1232
AsuC2I	CCSGG	1 cut(s)	297
AsuHPI	GGTGA	4 cut(s)	220, 403, 724, 989
AsuII	TTCGAA	1 cut(s)	539
AvaII	GGWCC	4 cut(s)	13, 364, 917, 1232
BaeI	ACNNNNGTAYC	2 cut(s)	1010, 1043
BalI	TGGCCA	3 cut(s)	513, 525, 1005
BanI	GGYRCC	1 cut(s)	672
BanII	GRGCYC	2 cut(s)	125, 499
BarI	GAAGNNNNNNTAC	2 cut(s)	1002, 1034
BbsI	GAAGAC	1 cut(s)	1017
Bbv12I	GWGCWC	1 cut(s)	125
BbvI	GCAGC	6 cut(s)	205, 322, 409, 415, 460, 1196
BccI	CCATC	4 cut(s)	177, 263, 603, 1360
BceAI	ACGGC	2 cut(s)	265, 295
BciT130I	CCWGG	1 cut(s)	786
BclI	TGATCA	1 cut(s)	1092
BcnI	CCSGG	1 cut(s)	297
BcoDI	GTCTC	1 cut(s)	1074
BfaI	CTAG	2 cut(s)	81, 1332
BfmI	CTRYAG	1 cut(s)	1210
BfoI	RGCGCY	1 cut(s)	204
BfrI	CTTAAG	1 cut(s)	932
Bme1390I	CCNGG	2 cut(s)	297, 786
Bme18I	GGWCC	4 cut(s)	13, 364, 917, 1232
BmgT120I	GGNCC	4 cut(s)	13, 364, 917, 1232
BmiI	GGNNCC	8 cut(s)	14, 56, 365, 366, 674, 799, 918, 1120
BmrFI	CCNGG	2 cut(s)	297, 786
BmrI	ACTGGG	1 cut(s)	964
BmsI	GCATC	2 cut(s)	580, 1003
BmuI	ACTGGG	1 cut(s)	964
BpiI	GAAGAC	1 cut(s)	1017
Bpu10I	CCTNAGC	1 cut(s)	477
Bpu14I	TTCGAA	1 cut(s)	539
BpuMI	CCSGG	1 cut(s)	297
BsaJI	CCNNGG	5 cut(s)	368, 526, 643, 1000, 1022
BsaWI	WCCGGW	1 cut(s)	205
Bsc4I	CCNNNNNNNGG	4 cut(s)	124, 605, 1199, 1337
Bse118I	RCCGGY	1 cut(s)	1192
Bse1I	ACTGG	3 cut(s)	799, 959, 1077
Bse3DI	GCAATG	1 cut(s)	885
BseBI	CCWGG	1 cut(s)	786
BseDI	CCNNGG	5 cut(s)	368, 526, 643, 1000, 1022
BseGI	GGATG	3 cut(s)	403, 614, 1101
BseLI	CCNNNNNNNGG	4 cut(s)	124, 605, 1199, 1337
BseMI	GCAATG	1 cut(s)	885
BseMII	CTCAG	5 cut(s)	125, 491, 675, 1043, 1170
BseNI	ACTGG	3 cut(s)	799, 959, 1077
BseRI	GAGGAG	4 cut(s)	245, 248, 251, 293
BseXI	GCAGC	6 cut(s)	205, 322, 409, 415, 460, 1196
BseYI	CCCAGC	1 cut(s)	884
BshFI	GGCC	6 cut(s)	30, 323, 460, 513, 525, 1005
BshNI	GGYRCC	1 cut(s)	672
BsiHKAI	GWGCWC	1 cut(s)	125
BsiSI	CCGG	3 cut(s)	206, 297, 1193
BslFI	GGGAC	3 cut(s)	377, 496, 815
BslI	CCNNNNNNNGG	4 cut(s)	124, 605, 1199, 1337
BsmAI	GTCTC	1 cut(s)	1074
BsmFI	GGGAC	3 cut(s)	377, 496, 815
BsmI	GAATGC	3 cut(s)	29, 580, 945
BsnI	GGCC	6 cut(s)	30, 323, 460, 513, 525, 1005
Bsp119I	TTCGAA	1 cut(s)	539
Bsp1286I	GDGCHC	2 cut(s)	125, 499
Bsp143I	GATC	5 cut(s)	130, 292, 820, 1075, 1092
Bsp19I	CCATGG	3 cut(s)	526, 1000, 1022
BspANI	GGCC	6 cut(s)	30, 323, 460, 513, 525, 1005
BspCNI	CTCAG	5 cut(s)	126, 490, 676, 1044, 1171
BspLI	GGNNCC	8 cut(s)	14, 56, 365, 366, 674, 799, 918, 1120
BspPI	GGATC	3 cut(s)	138, 287, 1083
BspQI	GCTCTTC	1 cut(s)	809
BspT104I	TTCGAA	1 cut(s)	539
BspT107I	GGYRCC	1 cut(s)	672
BspTI	CTTAAG	1 cut(s)	932
BsrDI	GCAATG	1 cut(s)	885
BsrFI	RCCGGY	1 cut(s)	1192
BsrI	ACTGG	3 cut(s)	799, 959, 1077
BssAI	RCCGGY	1 cut(s)	1192
BssECI	CCNNGG	5 cut(s)	368, 526, 643, 1000, 1022
BssMI	GATC	5 cut(s)	130, 292, 820, 1075, 1092
BssT1I	CCWWGG	3 cut(s)	526, 1000, 1022
Bst2UI	CCWGG	1 cut(s)	786
Bst4CI	ACNGT	4 cut(s)	560, 647, 736, 865
Bst6I	CTCTTC	3 cut(s)	222, 270, 809
BstAFI	CTTAAG	1 cut(s)	932
BstAPI	GCANNNNNTGC	1 cut(s)	577
BstBI	TTCGAA	1 cut(s)	539
BstC8I	GCNNGC	1 cut(s)	753
BstDEI	CTNAG	6 cut(s)	134, 477, 684, 1052, 1165, 1179
BstDSI	CCRYGG	5 cut(s)	368, 526, 643, 1000, 1022
BstF5I	GGATG	3 cut(s)	403, 614, 1101
BstH2I	RGCGCY	1 cut(s)	204
BstHHI	GCGC	3 cut(s)	203, 304, 729
BstKTI	GATC	5 cut(s)	133, 295, 823, 1078, 1095
BstMAI	GTCTC	1 cut(s)	1074
BstMBI	GATC	5 cut(s)	130, 292, 820, 1075, 1092
BstMWI	GCNNNNNNNGC	8 cut(s)	43, 258, 428, 531, 577, 1181, 1302, 1341
BstNI	CCWGG	1 cut(s)	786
BstSCI	CCNGG	2 cut(s)	295, 784
BstSFI	CTRYAG	1 cut(s)	1210
BstV1I	GCAGC	6 cut(s)	205, 322, 409, 415, 460, 1196
BstV2I	GAAGAC	1 cut(s)	1017
BstX2I	RGATCY	1 cut(s)	130
BstXI	CCANNNNNNTGG	1 cut(s)	521
BstYI	RGATCY	1 cut(s)	130
BsuRI	GGCC	6 cut(s)	30, 323, 460, 513, 525, 1005
BtgI	CCRYGG	5 cut(s)	368, 526, 643, 1000, 1022
BtgZI	GCGATG	1 cut(s)	1320
BtsCI	GGATG	3 cut(s)	403, 614, 1101
BtsIMutI	CAGTG	2 cut(s)	565, 1070
Cac8I	GCNNGC	1 cut(s)	753
CfoI	GCGC	3 cut(s)	203, 304, 729
Cfr10I	RCCGGY	1 cut(s)	1192
Cfr13I	GGNCC	4 cut(s)	13, 364, 917, 1232
Csp6I	GTAC	3 cut(s)	596, 737, 1315
CviQI	GTAC	3 cut(s)	596, 737, 1315
DdeI	CTNAG	6 cut(s)	134, 477, 684, 1052, 1165, 1179
DpnI	GATC	5 cut(s)	132, 294, 822, 1077, 1094
DpnII	GATC	5 cut(s)	130, 292, 820, 1075, 1092
DraIII	CACNNNGTG	1 cut(s)	1291
EaeI	YGGCCR	4 cut(s)	321, 511, 523, 1003
Eam1104I	CTCTTC	3 cut(s)	222, 270, 809
EarI	CTCTTC	3 cut(s)	222, 270, 809
EciI	GGCGGA	2 cut(s)	143, 279
Ecl136II	GAGCTC	1 cut(s)	123
Eco130I	CCWWGG	3 cut(s)	526, 1000, 1022
Eco24I	GRGCYC	2 cut(s)	125, 499
Eco47I	GGWCC	4 cut(s)	13, 364, 917, 1232
Eco47III	AGCGCT	1 cut(s)	202
Eco53kI	GAGCTC	1 cut(s)	123
EcoICRI	GAGCTC	1 cut(s)	123
EcoO109I	RGGNCCY	4 cut(s)	13, 364, 917, 1232
EcoRII	CCWGG	1 cut(s)	784
EcoT14I	CCWWGG	3 cut(s)	526, 1000, 1022
EcoT38I	GRGCYC	2 cut(s)	125, 499
ErhI	CCWWGG	3 cut(s)	526, 1000, 1022
FaqI	GGGAC	3 cut(s)	377, 496, 815
FauI	CCCGC	1 cut(s)	51
FbaI	TGATCA	1 cut(s)	1092
FokI	GGATG	3 cut(s)	410, 621, 1088
FriOI	GRGCYC	2 cut(s)	125, 499
FspBI	CTAG	2 cut(s)	81, 1332
GlaI	GCGC	3 cut(s)	202, 303, 728
GsaI	CCCAGC	1 cut(s)	888
HaeII	RGCGCY	1 cut(s)	204
HaeIII	GGCC	6 cut(s)	30, 323, 460, 513, 525, 1005
HapII	CCGG	3 cut(s)	206, 297, 1193
HhaI	GCGC	3 cut(s)	203, 304, 729
Hin6I	GCGC	3 cut(s)	201, 302, 727
HinP1I	GCGC	3 cut(s)	201, 302, 727
HincII	GTYRAC	1 cut(s)	247
HindII	GTYRAC	1 cut(s)	247
HindIII	AAGCTT	2 cut(s)	532, 1270
HinfI	GANTC	3 cut(s)	143, 628, 665
HpaI	GTTAAC	1 cut(s)	247
HpaII	CCGG	3 cut(s)	206, 297, 1193
HphI	GGTGA	4 cut(s)	220, 403, 724, 989
Hpy166II	GTNNAC	3 cut(s)	247, 442, 1315
Hpy188I	TCNGA	8 cut(s)	22, 102, 135, 158, 345, 685, 1053, 1118
Hpy188III	TCNNGA	5 cut(s)	655, 832, 1079, 1201, 1230
Hpy8I	GTNNAC	3 cut(s)	247, 442, 1315
Hpy99I	CGWCG	1 cut(s)	329
HpyAV	CCTTC	1 cut(s)	1030
HpyCH4III	ACNGT	4 cut(s)	560, 647, 736, 865
HpyCH4IV	ACGT	2 cut(s)	195, 327
HpyCH4V	TGCA	2 cut(s)	580, 994
HpyF10VI	GCNNNNNNNGC	8 cut(s)	43, 258, 428, 531, 577, 1181, 1302, 1341
HpyF3I	CTNAG	6 cut(s)	134, 477, 684, 1052, 1165, 1179
HpySE526I	ACGT	2 cut(s)	195, 327
HspAI	GCGC	3 cut(s)	201, 302, 727
KflI	GGGWCCC	1 cut(s)	364
Ksp22I	TGATCA	1 cut(s)	1092
KspAI	GTTAAC	1 cut(s)	247
Kzo9I	GATC	5 cut(s)	130, 292, 820, 1075, 1092
LguI	GCTCTTC	1 cut(s)	809
LmnI	GCTCC	1 cut(s)	208
Lsp1109I	GCAGC	6 cut(s)	205, 322, 409, 415, 460, 1196
LweI	GCATC	2 cut(s)	580, 1003
MaeI	CTAG	2 cut(s)	81, 1332
MaeII	ACGT	2 cut(s)	195, 327
MaeIII	GTNAC	3 cut(s)	850, 888, 982
MalI	GATC	5 cut(s)	132, 294, 822, 1077, 1094
MboI	GATC	5 cut(s)	130, 292, 820, 1075, 1092
MboII	GAAGA	7 cut(s)	192, 239, 287, 576, 826, 1022, 1234
MflI	RGATCY	1 cut(s)	130
MhlI	GDGCHC	2 cut(s)	125, 499
MlsI	TGGCCA	3 cut(s)	513, 525, 1005
MluCI	AATT	7 cut(s)	317, 588, 615, 701, 720, 1016, 1357
MluNI	TGGCCA	3 cut(s)	513, 525, 1005
MlyI	GAGTC	2 cut(s)	622, 674
MmeI	TCCRAC	1 cut(s)	851
Mox20I	TGGCCA	3 cut(s)	513, 525, 1005
MroXI	GAANNNNTTC	1 cut(s)	535
MscI	TGGCCA	3 cut(s)	513, 525, 1005
MseI	TTAA	5 cut(s)	246, 255, 330, 933, 1360
MslI	CAYNNNNRTG	3 cut(s)	519, 648, 999
Msp20I	TGGCCA	3 cut(s)	513, 525, 1005
MspCI	CTTAAG	1 cut(s)	932
MspI	CCGG	3 cut(s)	206, 297, 1193
MspR9I	CCNGG	2 cut(s)	297, 786
Mva1269I	GAATGC	3 cut(s)	29, 580, 945
MvaI	CCWGG	1 cut(s)	786
MwoI	GCNNNNNNNGC	8 cut(s)	43, 258, 428, 531, 577, 1181, 1302, 1341
NciI	CCSGG	1 cut(s)	297
NcoI	CCATGG	3 cut(s)	526, 1000, 1022
NdeII	GATC	5 cut(s)	130, 292, 820, 1075, 1092
NlaIV	GGNNCC	8 cut(s)	14, 56, 365, 366, 674, 799, 918, 1120
NmeAIII	GCCGAG	1 cut(s)	104
NmuCI	GTSAC	1 cut(s)	850
NspV	TTCGAA	1 cut(s)	539
PciSI	GCTCTTC	1 cut(s)	809
PcsI	WCGNNNNNNNCGW	2 cut(s)	651, 660
PctI	GAATGC	3 cut(s)	29, 580, 945
PdmI	GAANNNNTTC	1 cut(s)	535
PfeI	GAWTC	1 cut(s)	143
PfoI	TCCNGGA	1 cut(s)	784
PleI	GAGTC	2 cut(s)	622, 673
PpsI	GAGTC	2 cut(s)	622, 673
PpuMI	RGGWCCY	4 cut(s)	13, 364, 917, 1232
Psp124BI	GAGCTC	1 cut(s)	125
Psp5II	RGGWCCY	4 cut(s)	13, 364, 917, 1232
Psp6I	CCWGG	1 cut(s)	784
PspFI	CCCAGC	1 cut(s)	884
PspGI	CCWGG	1 cut(s)	784
PspN4I	GGNNCC	8 cut(s)	14, 56, 365, 366, 674, 799, 918, 1120
PspPI	GGNCC	4 cut(s)	13, 364, 917, 1232
PspPPI	RGGWCCY	4 cut(s)	13, 364, 917, 1232
PsuI	RGATCY	1 cut(s)	130
RsaI	GTAC	3 cut(s)	597, 738, 1316
RsaNI	GTAC	3 cut(s)	596, 737, 1315
RseI	CAYNNNNRTG	3 cut(s)	519, 648, 999
SacI	GAGCTC	1 cut(s)	125
SapI	GCTCTTC	1 cut(s)	809
SaqAI	TTAA	5 cut(s)	246, 255, 330, 933, 1360
Sau3AI	GATC	5 cut(s)	130, 292, 820, 1075, 1092
Sau96I	GGNCC	4 cut(s)	13, 364, 917, 1232
SchI	GAGTC	2 cut(s)	622, 674
ScrFI	CCNGG	2 cut(s)	297, 786
SduI	GDGCHC	2 cut(s)	125, 499
SfaNI	GCATC	2 cut(s)	580, 1003
SfcI	CTRYAG	1 cut(s)	1210
SfuI	TTCGAA	1 cut(s)	539
SinI	GGWCC	4 cut(s)	13, 364, 917, 1232
SmiMI	CAYNNNNRTG	3 cut(s)	519, 648, 999
SmlI	CTYRAG	1 cut(s)	932
SmoI	CTYRAG	1 cut(s)	932
Sse9I	AATT	7 cut(s)	317, 588, 615, 701, 720, 1016, 1357
SspMI	CTAG	2 cut(s)	81, 1332
SstI	GAGCTC	1 cut(s)	125
StyD4I	CCNGG	2 cut(s)	295, 784
StyI	CCWWGG	3 cut(s)	526, 1000, 1022
TaaI	ACNGT	4 cut(s)	560, 647, 736, 865
TaiI	ACGT	2 cut(s)	198, 330
TaqI	TCGA	5 cut(s)	146, 539, 654, 1030, 1078
TasI	AATT	7 cut(s)	317, 588, 615, 701, 720, 1016, 1357
TatI	WGTACW	1 cut(s)	1314
TauI	GCSGC	5 cut(s)	30, 264, 307, 310, 428
TfiI	GAWTC	1 cut(s)	143
Tru1I	TTAA	5 cut(s)	246, 255, 330, 933, 1360
Tru9I	TTAA	5 cut(s)	246, 255, 330, 933, 1360
TscAI	CASTG	2 cut(s)	565, 1077
TseFI	GTSAC	1 cut(s)	850
TseI	GCWGC	6 cut(s)	218, 335, 422, 428, 473, 1184
Tsp45I	GTSAC	1 cut(s)	850
TspDTI	ATGAA	1 cut(s)	1121
TspGWI	ACGGA	1 cut(s)	1101
TspRI	CASTG	2 cut(s)	565, 1077
Vha464I	CTTAAG	1 cut(s)	932
VpaK11BI	GGWCC	4 cut(s)	13, 364, 917, 1232
XapI	RAATTY	1 cut(s)	720
XmnI	GAANNNNTTC	1 cut(s)	535
XspI	CTAG	2 cut(s)	81, 1332

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.