Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002181

Physical Location & Seq

Genomic Coordinates Reverse (-)

24313422 .. 24316242

Gene Structure 2821 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1155400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1365 bp

ATGGCGAGTCGGGTCCTCTCAGAATGCGGACTAAAACCTCTCCCCAAAGTTTGGCGCGGACCCGGAACCGCAATTTGCTGTAGCCACCGGTCAAAAGTTCGAATTTTTCGGTCCAACCAGAGCTCGACGGATCTGAGATTGGATTCCACCAAAATCCCAGTTGGGCCGTTCAGAGAGAGAAGATGGGGACTAAACGTGAGCGCTCCGTTGAGAGTTGCTTCATTTGAAGAGGAGGAGGAGAGGGTTAACGGCGTTAATGGTGGCGGAGATGGAGAAGAGGAGGTGGGATTTGATCCGGGTGCGCCGCCGCCGTTTAAATTGGCTGACGTTAAAGCTGCCATTCCGAAGCATTGTTGGGTTAAGGACCCCTGGAGGTCAATGAGCTATGTGGTGAGGGATGTGGCGGTGGTTTTTGGGTTAGCTGCGGCCGCGGTTTATGTAAACAAGTGGTTTGTTTGGCCTCTGTATTGGGCTGCTCAGGGGACAATGTTTTGGGCTCTATTTGTCCTCGGCCATGATTGTGGCCATGGAAGCTTTTCGAATAATCCGAAGCTAAACAGTGTGGTGGGGCATCTCTTGCATTCATCAATTCTTGTACCCTATCATGGATGGAGAATTAGCCATAGGACTCATCATCAGAACCATGGCCATGTTGAGAATGACGAGTCGTGGCACCCATTGTCTGAGAAAATCTACAAGAACTTGGATAACATGACACGAATTTTGCGCTTCACCATGCCTTTTCCCATGCTTGCATACCCTTTCTACCTCTGGAATAGAAGTCCAGGAAAGACTGGTTCCCACTTTCACCCGGACAGCGATCTGTTTGTCAAGAAAGAGAGGAAAGATGTGATCACTTCCAGTGTATGTTGGACAGCAATGGCCGCGCTACTTGTGGGTTTGTCCTTTGTAATGGGTCCTGTTCAAGTTCTTAAGCTCTACGGCATTCCCTATTGGGTTTTTGTCATGTGGCTGGATTTGGTAACGTACTTGCATCACCATGGTCATGAAGACAAACTACCCTGGTATCGAGGAAAGGAATGGAGTTATCTGAGAGGAGGGCTTACGACACTGGATCGTGACTATGGATTGATCAATAACATCCACCATGACATTGGAACCCATGTGGTTCATCATCTTTTTCCTCAGATCCCCCACTACCATTTAGTTGAAGCAACCGAGGCAGCAAAGCCAGTATTCGGGAAATACTACAGAGAACCAAAGAAAGCAGGACCTCTACCATTTCACTTGCTTGGAAGTTTTATAAGAAGCTTGAAAAAAGACCACTACGTGAGTGACACTGGCGATGTTGTGTACTACCAAACCGACCCTAACTTTGGTGGCAGGAATCCTGCATCGAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3474 FA_desaturase

Protein Analysis

455

Amino Acids

51.71

Weight (kDa)

9.08

Isoelectric Point (pI)

38.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF3474	PF11960	1 - 142	8.2e-56	Domain of unknown function (DUF3474)
FA_desaturase	PF00487	150 - 404	5.7e-32	Fatty acid desaturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002181)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11170 AT5G05580 AT5G05580
fragaria_vesca	FvH4_6g11310 FvH4_6g11310 FvH4_6g11310 FvH4_6g11310 FvH4_7g24590 FvH4_7g24590 FvH4_7g24590
malus_domestica	MD01G1154100.v1.1 MD04G1155400.v1.1 MD07G1223100.v1.1 MD12G1168600.v1.1 MD17G1258700.v1.1
prunus_persica	Prupe.2G251200_v2.0.a1 Prupe.6G278800_v2.0.a1 Prupe.6G278800_v2.0.a1
pyrus_communis	pycom01g17140 pycom04g13980 pycom07g20170 pycom12g16010
rosa_chinensis	RchiOBHm_Chr1g0370421 RchiOBHm_Chr3g0462851
rosa_laevigata	RLG00000024827 RLG00000027049
rosa_multiflora	Rmu_sc0000766.1_g000013 Rmu_sc0005656.1_g000006
rosa_roxburghii	Rroxscaffold_4G00286590 Rroxscaffold_6G00417140
rosa_rugosa	Rorug01G0356300 Rorug03G0059600
rosa_samantha	Rh1AG364800 Rh1BG328000 Rh1CG341000 Rh1DG358300 Rh3AG117800 Rh3BG121600 Rh3CG124500 Rh3DG123200
rosa_wichuraiana	Rw1G032100 Rw1G032150 Rw3G009990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1265
AccB1I	GGYRCC	1 cut(s)	672
AccB7I	CCANNNNNTGG	1 cut(s)	51
AccII	CGCG	3 cut(s)	57, 431, 887
AclWI	GGATC	4 cut(s)	138, 287, 1083, 1144
AcoI	YGGCCR	5 cut(s)	426, 511, 523, 646, 882
AcsI	RAATTY	2 cut(s)	102, 720
AdeI	CACNNNGTG	1 cut(s)	1291
AfaI	GTAC	3 cut(s)	597, 989, 1316
AfeI	AGCGCT	1 cut(s)	202
AfiI	CCNNNNNNNGG	4 cut(s)	51, 605, 1199, 1337
AflII	CTTAAG	1 cut(s)	932
AgeI	ACCGGT	1 cut(s)	87
AgsI	TTSAA	4 cut(s)	227, 926, 1172, 1276
AjnI	CCWGG	3 cut(s)	368, 784, 1022
AluBI	AGCT	8 cut(s)	123, 335, 384, 422, 534, 553, 937, 1272
AluI	AGCT	8 cut(s)	123, 335, 384, 422, 534, 553, 937, 1272
Alw21I	GWGCWC	1 cut(s)	125
AlwI	GGATC	4 cut(s)	138, 287, 1083, 1144
Aor51HI	AGCGCT	1 cut(s)	202
AoxI	GGCC	7 cut(s)	164, 426, 458, 511, 523, 646, 882
ApeKI	GCWGC	4 cut(s)	335, 422, 473, 1184
ApoI	RAATTY	2 cut(s)	102, 720
ArsI	GACNNNNNNTTYG	4 cut(s)	706, 738, 1319, 1351
AsiGI	ACCGGT	1 cut(s)	87
Asp700I	GAANNNNTTC	1 cut(s)	535
AspLEI	GCGC	5 cut(s)	57, 203, 304, 729, 889
AspS9I	GGNCC	7 cut(s)	13, 59, 111, 164, 364, 917, 1232
AsuC2I	CCSGG	3 cut(s)	63, 297, 812
AsuHPI	GGTGA	4 cut(s)	403, 724, 800, 989
AsuII	TTCGAA	2 cut(s)	100, 539
AvaII	GGWCC	6 cut(s)	13, 59, 111, 364, 917, 1232
BaeI	ACNNNNGTAYC	2 cut(s)	1010, 1043
BalI	TGGCCA	2 cut(s)	525, 648
BanI	GGYRCC	1 cut(s)	672
BanII	GRGCYC	2 cut(s)	125, 499
BarI	GAAGNNNNNNTAC	2 cut(s)	1002, 1034
BbsI	GAAGAC	1 cut(s)	1017
Bbv12I	GWGCWC	1 cut(s)	125
BbvI	GCAGC	4 cut(s)	322, 409, 460, 1196
BccI	CCATC	3 cut(s)	177, 263, 603
BceAI	ACGGC	4 cut(s)	151, 265, 295, 958
BciT130I	CCWGG	3 cut(s)	370, 786, 1024
BclI	TGATCA	2 cut(s)	852, 1092
BcnI	CCSGG	3 cut(s)	63, 297, 812
BfmI	CTRYAG	2 cut(s)	79, 1210
BfoI	RGCGCY	1 cut(s)	204
BfrI	CTTAAG	1 cut(s)	932
BisI	GCNGC	9 cut(s)	305, 308, 336, 423, 426, 429, 474, 885, 1185
BlsI	GCNGC	9 cut(s)	306, 309, 337, 424, 427, 430, 475, 886, 1186
Bme1390I	CCNGG	6 cut(s)	63, 297, 370, 786, 812, 1024
Bme18I	GGWCC	6 cut(s)	13, 59, 111, 364, 917, 1232
BmgT120I	GGNCC	7 cut(s)	13, 59, 111, 164, 364, 917, 1232
BmiI	GGNNCC	8 cut(s)	14, 61, 67, 366, 674, 799, 918, 1120
BmrFI	CCNGG	6 cut(s)	63, 297, 370, 786, 812, 1024
BmrI	ACTGGG	1 cut(s)	152
BmsI	GCATC	2 cut(s)	580, 1003
BmuI	ACTGGG	1 cut(s)	152
BpiI	GAAGAC	1 cut(s)	1017
BpmI	CTGGAG	1 cut(s)	391
Bpu10I	CCTNAGC	1 cut(s)	477
Bpu14I	TTCGAA	2 cut(s)	100, 539
BpuMI	CCSGG	3 cut(s)	63, 297, 812
BsaAI	YACGTR	1 cut(s)	1291
BsaJI	CCNNGG	8 cut(s)	368, 429, 508, 526, 643, 1000, 1022, 1179
BsaWI	WCCGGW	1 cut(s)	87
Bsc4I	CCNNNNNNNGG	4 cut(s)	51, 605, 1199, 1337
Bse118I	RCCGGY	1 cut(s)	87
Bse1I	ACTGG	6 cut(s)	158, 799, 861, 1077, 1193, 1306
Bse3DI	GCAATG	1 cut(s)	885
BseBI	CCWGG	3 cut(s)	370, 786, 1024
BseDI	CCNNGG	8 cut(s)	368, 429, 508, 526, 643, 1000, 1022, 1179
BseGI	GGATG	3 cut(s)	403, 614, 1101
BseLI	CCNNNNNNNGG	4 cut(s)	51, 605, 1199, 1337
BseMI	GCAATG	1 cut(s)	885
BseMII	CTCAG	6 cut(s)	33, 125, 491, 675, 1043, 1160
BseNI	ACTGG	6 cut(s)	158, 799, 861, 1077, 1193, 1306
BseRI	GAGGAG	5 cut(s)	245, 248, 251, 293, 1071
BseX3I	CGGCCG	1 cut(s)	426
BseXI	GCAGC	4 cut(s)	322, 409, 460, 1196
Bsh1236I	CGCG	3 cut(s)	57, 431, 887
Bsh1285I	CGRYCG	1 cut(s)	429
BshFI	GGCC	7 cut(s)	166, 428, 460, 513, 525, 648, 884
BshNI	GGYRCC	1 cut(s)	672
BshTI	ACCGGT	1 cut(s)	87
BsiEI	CGRYCG	1 cut(s)	429
BsiHKAI	GWGCWC	1 cut(s)	125
BsiSI	CCGG	4 cut(s)	63, 88, 296, 812
BslFI	GGGAC	2 cut(s)	201, 496
BslI	CCNNNNNNNGG	4 cut(s)	51, 605, 1199, 1337
BsmFI	GGGAC	2 cut(s)	201, 496
BsmI	GAATGC	3 cut(s)	29, 580, 945
BsnI	GGCC	7 cut(s)	166, 428, 460, 513, 525, 648, 884
Bsp119I	TTCGAA	2 cut(s)	100, 539
Bsp1286I	GDGCHC	2 cut(s)	125, 499
Bsp143I	GATC	7 cut(s)	130, 292, 820, 852, 1075, 1092, 1149
Bsp19I	CCATGG	3 cut(s)	526, 643, 1000
BspANI	GGCC	7 cut(s)	166, 428, 460, 513, 525, 648, 884
BspCNI	CTCAG	6 cut(s)	32, 126, 490, 676, 1044, 1159
BspFNI	CGCG	3 cut(s)	57, 431, 887
BspHI	TCATGA	1 cut(s)	1006
BspLI	GGNNCC	8 cut(s)	14, 61, 67, 366, 674, 799, 918, 1120
BspPI	GGATC	4 cut(s)	138, 287, 1083, 1144
BspT104I	TTCGAA	2 cut(s)	100, 539
BspT107I	GGYRCC	1 cut(s)	672
BspTI	CTTAAG	1 cut(s)	932
BsrDI	GCAATG	1 cut(s)	885
BsrFI	RCCGGY	1 cut(s)	87
BsrI	ACTGG	6 cut(s)	158, 799, 861, 1077, 1193, 1306
BssAI	RCCGGY	1 cut(s)	87
BssECI	CCNNGG	8 cut(s)	368, 429, 508, 526, 643, 1000, 1022, 1179
BssMI	GATC	7 cut(s)	130, 292, 820, 852, 1075, 1092, 1149
BssT1I	CCWWGG	3 cut(s)	526, 643, 1000
Bst2UI	CCWGG	3 cut(s)	370, 786, 1024
Bst4CI	ACNGT	1 cut(s)	560
Bst6I	CTCTTC	2 cut(s)	222, 270
BstAFI	CTTAAG	1 cut(s)	932
BstAPI	GCANNNNNTGC	1 cut(s)	577
BstBAI	YACGTR	1 cut(s)	1291
BstBI	TTCGAA	2 cut(s)	100, 539
BstC8I	GCNNGC	1 cut(s)	753
BstDEI	CTNAG	6 cut(s)	19, 134, 477, 684, 1052, 1146
BstDSI	CCRYGG	4 cut(s)	429, 526, 643, 1000
BstF5I	GGATG	3 cut(s)	403, 614, 1101
BstFNI	CGCG	3 cut(s)	57, 431, 887
BstH2I	RGCGCY	1 cut(s)	204
BstHHI	GCGC	5 cut(s)	57, 203, 304, 729, 889
BstKTI	GATC	7 cut(s)	133, 295, 823, 855, 1078, 1095, 1152
BstMBI	GATC	7 cut(s)	130, 292, 820, 852, 1075, 1092, 1149
BstMCI	CGRYCG	1 cut(s)	429
BstMWI	GCNNNNNNNGC	5 cut(s)	428, 531, 577, 884, 1181
BstNI	CCWGG	3 cut(s)	370, 786, 1024
BstSCI	CCNGG	6 cut(s)	61, 295, 368, 784, 810, 1022
BstSFI	CTRYAG	2 cut(s)	79, 1210
BstUI	CGCG	3 cut(s)	57, 431, 887
BstV1I	GCAGC	4 cut(s)	322, 409, 460, 1196
BstV2I	GAAGAC	1 cut(s)	1017
BstX2I	RGATCY	2 cut(s)	130, 1149
BstXI	CCANNNNNNTGG	2 cut(s)	521, 1115
BstYI	RGATCY	2 cut(s)	130, 1149
BstZI	CGGCCG	1 cut(s)	426
BsuRI	GGCC	7 cut(s)	166, 428, 460, 513, 525, 648, 884
BtgI	CCRYGG	4 cut(s)	429, 526, 643, 1000
BtgZI	GCGATG	1 cut(s)	1320
BtsCI	GGATG	3 cut(s)	403, 614, 1101
BtsIMutI	CAGTG	4 cut(s)	565, 868, 1070, 1299
Cac8I	GCNNGC	1 cut(s)	753
CciI	TCATGA	1 cut(s)	1006
CciNI	GCGGCCGC	1 cut(s)	426
CfoI	GCGC	5 cut(s)	57, 203, 304, 729, 889
Cfr10I	RCCGGY	1 cut(s)	87
Cfr13I	GGNCC	7 cut(s)	13, 59, 111, 164, 364, 917, 1232
Cfr42I	CCGCGG	1 cut(s)	432
Csp6I	GTAC	3 cut(s)	596, 988, 1315
CspAI	ACCGGT	1 cut(s)	87
CviQI	GTAC	3 cut(s)	596, 988, 1315
DdeI	CTNAG	6 cut(s)	19, 134, 477, 684, 1052, 1146
DpnI	GATC	7 cut(s)	132, 294, 822, 854, 1077, 1094, 1151
DpnII	GATC	7 cut(s)	130, 292, 820, 852, 1075, 1092, 1149
DraI	TTTAAA	1 cut(s)	316
DraIII	CACNNNGTG	1 cut(s)	1291
EaeI	YGGCCR	5 cut(s)	426, 511, 523, 646, 882
EagI	CGGCCG	1 cut(s)	426
Eam1104I	CTCTTC	2 cut(s)	222, 270
EarI	CTCTTC	2 cut(s)	222, 270
EciI	GGCGGA	1 cut(s)	279
Ecl136II	GAGCTC	1 cut(s)	123
EclXI	CGGCCG	1 cut(s)	426
Eco130I	CCWWGG	3 cut(s)	526, 643, 1000
Eco24I	GRGCYC	2 cut(s)	125, 499
Eco47I	GGWCC	6 cut(s)	13, 59, 111, 364, 917, 1232
Eco47III	AGCGCT	1 cut(s)	202
Eco52I	CGGCCG	1 cut(s)	426
Eco53kI	GAGCTC	1 cut(s)	123
EcoICRI	GAGCTC	1 cut(s)	123
EcoO109I	RGGNCCY	4 cut(s)	13, 364, 917, 1232
EcoRII	CCWGG	3 cut(s)	368, 784, 1022
EcoT14I	CCWWGG	3 cut(s)	526, 643, 1000
EcoT38I	GRGCYC	2 cut(s)	125, 499
ErhI	CCWWGG	3 cut(s)	526, 643, 1000
FaqI	GGGAC	2 cut(s)	201, 496
FbaI	TGATCA	2 cut(s)	852, 1092
Fnu4HI	GCNGC	9 cut(s)	305, 308, 336, 423, 426, 429, 474, 885, 1185
FokI	GGATG	3 cut(s)	410, 621, 1088
FriOI	GRGCYC	2 cut(s)	125, 499
Fsp4HI	GCNGC	9 cut(s)	305, 308, 336, 423, 426, 429, 474, 885, 1185
GlaI	GCGC	5 cut(s)	56, 202, 303, 728, 888
GluI	GCNGC	9 cut(s)	305, 308, 336, 423, 426, 429, 474, 885, 1185
GsuI	CTGGAG	1 cut(s)	391
HaeII	RGCGCY	1 cut(s)	204
HaeIII	GGCC	7 cut(s)	166, 428, 460, 513, 525, 648, 884
HapII	CCGG	4 cut(s)	63, 88, 296, 812
HhaI	GCGC	5 cut(s)	57, 203, 304, 729, 889
Hin6I	GCGC	5 cut(s)	55, 201, 302, 727, 887
HinP1I	GCGC	5 cut(s)	55, 201, 302, 727, 887
HincII	GTYRAC	1 cut(s)	247
HindII	GTYRAC	1 cut(s)	247
HindIII	AAGCTT	2 cut(s)	532, 1270
HinfI	GANTC	5 cut(s)	7, 143, 628, 665, 1348
HpaI	GTTAAC	1 cut(s)	247
HpaII	CCGG	4 cut(s)	63, 88, 296, 812
HphI	GGTGA	4 cut(s)	403, 724, 800, 989
Hpy166II	GTNNAC	3 cut(s)	247, 442, 1315
Hpy188I	TCNGA	9 cut(s)	22, 135, 173, 345, 549, 639, 685, 1053, 1149
Hpy188III	TCNNGA	5 cut(s)	772, 832, 1007, 1079, 1201
Hpy8I	GTNNAC	3 cut(s)	247, 442, 1315
Hpy99I	CGWCG	1 cut(s)	130
HpyCH4III	ACNGT	1 cut(s)	560
HpyCH4IV	ACGT	4 cut(s)	195, 327, 986, 1290
HpyCH4V	TGCA	4 cut(s)	580, 755, 994, 1355
HpyF10VI	GCNNNNNNNGC	5 cut(s)	428, 531, 577, 884, 1181
HpyF3I	CTNAG	6 cut(s)	19, 134, 477, 684, 1052, 1146
HpySE526I	ACGT	4 cut(s)	195, 327, 986, 1290
HspAI	GCGC	5 cut(s)	55, 201, 302, 727, 887
Ksp22I	TGATCA	2 cut(s)	852, 1092
KspAI	GTTAAC	1 cut(s)	247
KspI	CCGCGG	1 cut(s)	432
Kzo9I	GATC	7 cut(s)	130, 292, 820, 852, 1075, 1092, 1149
LmnI	GCTCC	1 cut(s)	208
Lsp1109I	GCAGC	4 cut(s)	322, 409, 460, 1196
LweI	GCATC	2 cut(s)	580, 1003
MaeII	ACGT	4 cut(s)	195, 327, 986, 1290
MaeIII	GTNAC	3 cut(s)	982, 1079, 1295
MalI	GATC	7 cut(s)	132, 294, 822, 854, 1077, 1094, 1151
MboI	GATC	7 cut(s)	130, 292, 820, 852, 1075, 1092, 1149
MboII	GAAGA	4 cut(s)	192, 239, 287, 1022
MflI	RGATCY	2 cut(s)	130, 1149
MhlI	GDGCHC	2 cut(s)	125, 499
MlsI	TGGCCA	2 cut(s)	525, 648
MluCI	AATT	7 cut(s)	72, 102, 317, 588, 615, 720, 1360
MluNI	TGGCCA	2 cut(s)	525, 648
MlyI	GAGTC	3 cut(s)	16, 622, 674
MmeI	TCCRAC	2 cut(s)	138, 851
Mox20I	TGGCCA	2 cut(s)	525, 648
MroXI	GAANNNNTTC	1 cut(s)	535
MscI	TGGCCA	2 cut(s)	525, 648
MseI	TTAA	7 cut(s)	246, 255, 315, 330, 360, 933, 1363
MslI	CAYNNNNRTG	4 cut(s)	519, 648, 999, 1005
Msp20I	TGGCCA	2 cut(s)	525, 648
MspA1I	CMGCKG	1 cut(s)	431
MspCI	CTTAAG	1 cut(s)	932
MspI	CCGG	4 cut(s)	63, 88, 296, 812
MspR9I	CCNGG	6 cut(s)	63, 297, 370, 786, 812, 1024
Mva1269I	GAATGC	3 cut(s)	29, 580, 945
MvaI	CCWGG	3 cut(s)	370, 786, 1024
MvnI	CGCG	3 cut(s)	57, 431, 887
MwoI	GCNNNNNNNGC	5 cut(s)	428, 531, 577, 884, 1181
NciI	CCSGG	3 cut(s)	63, 297, 812
NcoI	CCATGG	3 cut(s)	526, 643, 1000
NdeII	GATC	7 cut(s)	130, 292, 820, 852, 1075, 1092, 1149
NlaIV	GGNNCC	8 cut(s)	14, 61, 67, 366, 674, 799, 918, 1120
NmeAIII	GCCGAG	1 cut(s)	489
NmuCI	GTSAC	2 cut(s)	1079, 1295
NotI	GCGGCCGC	1 cut(s)	426
NspV	TTCGAA	2 cut(s)	100, 539
PagI	TCATGA	1 cut(s)	1006
PcsI	WCGNNNNNNNCGW	1 cut(s)	545
PctI	GAATGC	3 cut(s)	29, 580, 945
PdmI	GAANNNNTTC	1 cut(s)	535
PfeI	GAWTC	2 cut(s)	143, 1348
PflMI	CCANNNNNTGG	1 cut(s)	51
PfoI	TCCNGGA	1 cut(s)	784
PinAI	ACCGGT	1 cut(s)	87
PkrI	GCNGC	9 cut(s)	306, 309, 337, 424, 427, 430, 475, 886, 1186
PleI	GAGTC	3 cut(s)	15, 622, 673
PpsI	GAGTC	3 cut(s)	15, 622, 673
Ppu21I	YACGTR	1 cut(s)	1291
PpuMI	RGGWCCY	4 cut(s)	13, 364, 917, 1232
PsiI	TTATAA	1 cut(s)	1265
Psp124BI	GAGCTC	1 cut(s)	125
Psp5II	RGGWCCY	4 cut(s)	13, 364, 917, 1232
Psp6I	CCWGG	3 cut(s)	368, 784, 1022
PspGI	CCWGG	3 cut(s)	368, 784, 1022
PspN4I	GGNNCC	8 cut(s)	14, 61, 67, 366, 674, 799, 918, 1120
PspPI	GGNCC	7 cut(s)	13, 59, 111, 164, 364, 917, 1232
PspPPI	RGGWCCY	4 cut(s)	13, 364, 917, 1232
PsuI	RGATCY	2 cut(s)	130, 1149
RsaI	GTAC	3 cut(s)	597, 989, 1316
RsaNI	GTAC	3 cut(s)	596, 988, 1315
RseI	CAYNNNNRTG	4 cut(s)	519, 648, 999, 1005
SacI	GAGCTC	1 cut(s)	125
SacII	CCGCGG	1 cut(s)	432
SaqAI	TTAA	7 cut(s)	246, 255, 315, 330, 360, 933, 1363
SatI	GCNGC	9 cut(s)	305, 308, 336, 423, 426, 429, 474, 885, 1185
Sau3AI	GATC	7 cut(s)	130, 292, 820, 852, 1075, 1092, 1149
Sau96I	GGNCC	7 cut(s)	13, 59, 111, 164, 364, 917, 1232
SchI	GAGTC	3 cut(s)	16, 622, 674
ScrFI	CCNGG	6 cut(s)	63, 297, 370, 786, 812, 1024
SduI	GDGCHC	2 cut(s)	125, 499
SfaNI	GCATC	2 cut(s)	580, 1003
SfcI	CTRYAG	2 cut(s)	79, 1210
Sfr303I	CCGCGG	1 cut(s)	432
SfuI	TTCGAA	2 cut(s)	100, 539
SgrBI	CCGCGG	1 cut(s)	432
SinI	GGWCC	6 cut(s)	13, 59, 111, 364, 917, 1232
SmiMI	CAYNNNNRTG	4 cut(s)	519, 648, 999, 1005
SmlI	CTYRAG	1 cut(s)	932
SmoI	CTYRAG	1 cut(s)	932
Sse9I	AATT	7 cut(s)	72, 102, 317, 588, 615, 720, 1360
SstI	GAGCTC	1 cut(s)	125
StyD4I	CCNGG	6 cut(s)	61, 295, 368, 784, 810, 1022
StyI	CCWWGG	3 cut(s)	526, 643, 1000
TaaI	ACNGT	1 cut(s)	560
TaiI	ACGT	4 cut(s)	198, 330, 989, 1293
TaqI	TCGA	5 cut(s)	100, 125, 539, 1030, 1358
TaqII	GACCGA	1 cut(s)	99
TasI	AATT	7 cut(s)	72, 102, 317, 588, 615, 720, 1360
TatI	WGTACW	1 cut(s)	1314
TauI	GCSGC	5 cut(s)	307, 310, 428, 431, 887
TfiI	GAWTC	2 cut(s)	143, 1348
Tru1I	TTAA	7 cut(s)	246, 255, 315, 330, 360, 933, 1363
Tru9I	TTAA	7 cut(s)	246, 255, 315, 330, 360, 933, 1363
TscAI	CASTG	4 cut(s)	565, 868, 1077, 1306
TseFI	GTSAC	2 cut(s)	1079, 1295
TseI	GCWGC	4 cut(s)	335, 422, 473, 1184
Tsp45I	GTSAC	2 cut(s)	1079, 1295
TspDTI	ATGAA	4 cut(s)	210, 573, 1023, 1121
TspGWI	ACGGA	2 cut(s)	143, 195
TspRI	CASTG	4 cut(s)	565, 868, 1077, 1306
Van91I	CCANNNNNTGG	1 cut(s)	51
Vha464I	CTTAAG	1 cut(s)	932
VpaK11BI	GGWCC	6 cut(s)	13, 59, 111, 364, 917, 1232
XapI	RAATTY	2 cut(s)	102, 720
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1112
XmnI	GAANNNNTTC	1 cut(s)	535

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.