MD00G1002600.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0006418
Physical Location & Seq
Forward (+)
505653 .. 506910
1258 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1002600.v1.1.491

Sequence Viewer

Length: 741 bp
ATGTTTCCTAATTCATATCATCATGATCAAAGCAAAGATTTGGTATTCCAGAATTCCTTTATTGATGATTCCCACCATAATGATATGATGCAAGTACAAGATCTAATGCGGGGTCATGAGGTTTTTCCAGAGGAGAAGAATCCATCCCGTAATAAGATAATGCGAAAAGGCAAGCAGCAGCAGCAACAACAACATATGATATTATCACCTAATTCCAGTAATACTGGAGAAAATAATAGTGATGGTAAGACTGAAAGGAAGATCGTGCGTAGGGACAACGAACGCCAAAGAAGACAGCTTATGGCAGTTCTTAATTCATCACTTCGATCCCTCCTCCCCTGTGAATTGATCAAGGGAAAACGTTCAATCACCGAGCACATGAATGAGGCCGTCAATTATATAAATCACACGAAGGCGAAGATTGAAGAACTGAATGCCAAGAAGGAAAAGATTAAAAAGTTGTATGAATGTGATTCTAAAGATTTTGGTCTCGAAAATGAAAGTTCGGGTCACGATTTTCTTAAGTACTCTATCATGGTTCGCCCAACCTGTTTGGGGGCAGTGGAGGTTGTGATAAGTAGCAGCTGCGGCGAGGAGGAAGGGTTGCCTCTTTCGGGAGTGCTCAAAGTATTGCTTGCTGAAGGCCTTAGTGTTGTTGGGTGTGCTTCCACCAGAGTAAATGAAAGTTCGTTTCACAACATTCAGTGTTGGCAACGATTTGGCATGTCTGGATTTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

247

Amino Acids

28.04

Weight (kDa)

7.68

Isoelectric Point (pI)

56.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 86 - 136 7.1e-06 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 109, 588
AclI AACGTT 1 cut(s) 361
AclWI GGATC 1 cut(s) 321
AcsI RAATTY 1 cut(s) 52
AcuI CTGAAG 1 cut(s) 660
AfaI GTAC 2 cut(s) 96, 527
AfiI CCNNNNNNNGG 2 cut(s) 555, 614
AflII CTTAAG 1 cut(s) 521
AgsI TTSAA 2 cut(s) 366, 425
AluBI AGCT 2 cut(s) 298, 585
AluI AGCT 2 cut(s) 298, 585
Alw21I GWGCWC 2 cut(s) 378, 624
Alw26I GTCTC 1 cut(s) 494
AlwI GGATC 1 cut(s) 321
AoxI GGCC 2 cut(s) 387, 643
ApeKI GCWGC 5 cut(s) 175, 178, 181, 582, 585
ApoI RAATTY 1 cut(s) 52
Asp700I GAANNNNTTC 1 cut(s) 361
AsuHPI GGTGA 2 cut(s) 198, 361
BbsI GAAGAC 1 cut(s) 298
Bbv12I GWGCWC 2 cut(s) 378, 624
BbvI GCAGC 5 cut(s) 187, 190, 193, 572, 594
BccI CCATC 2 cut(s) 151, 236
BceAI ACGGC 1 cut(s) 374
BclI TGATCA 2 cut(s) 25, 348
BcoDI GTCTC 1 cut(s) 494
BfrI CTTAAG 1 cut(s) 521
BglII AGATCT 1 cut(s) 100
BisI GCNGC 6 cut(s) 176, 179, 182, 583, 586, 589
BlsI GCNGC 6 cut(s) 177, 180, 183, 584, 587, 590
BmcAI AGTACT 1 cut(s) 527
BmsI GCATC 1 cut(s) 78
BpiI GAAGAC 1 cut(s) 298
BpmI CTGGAG 1 cut(s) 246
BsaI GGTCTC 1 cut(s) 494
BsaXI ACNNNNNCTCC 2 cut(s) 587, 617
Bsc4I CCNNNNNNNGG 2 cut(s) 555, 614
Bse1I ACTGG 2 cut(s) 216, 229
BseGI GGATG 1 cut(s) 143
BseLI CCNNNNNNNGG 2 cut(s) 555, 614
BseNI ACTGG 2 cut(s) 216, 229
BseRI GAGGAG 3 cut(s) 146, 323, 608
BseXI GCAGC 5 cut(s) 187, 190, 193, 572, 594
BshFI GGCC 2 cut(s) 389, 645
BsiHKAI GWGCWC 2 cut(s) 378, 624
BslFI GGGAC 1 cut(s) 287
BslI CCNNNNNNNGG 2 cut(s) 555, 614
BsmAI GTCTC 1 cut(s) 494
BsmFI GGGAC 1 cut(s) 287
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 2 cut(s) 389, 645
Bso31I GGTCTC 1 cut(s) 494
Bsp1286I GDGCHC 2 cut(s) 378, 624
Bsp143I GATC 5 cut(s) 25, 100, 261, 326, 348
BspACI CCGC 2 cut(s) 109, 588
BspANI GGCC 2 cut(s) 389, 645
BspHI TCATGA 2 cut(s) 22, 115
BspPI GGATC 1 cut(s) 321
BspTI CTTAAG 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 494
BsrI ACTGG 2 cut(s) 216, 229
BssMI GATC 5 cut(s) 25, 100, 261, 326, 348
BstAFI CTTAAG 1 cut(s) 521
BstC8I GCNNGC 2 cut(s) 173, 636
BstDEI CTNAG 1 cut(s) 647
BstF5I GGATG 1 cut(s) 143
BstKTI GATC 5 cut(s) 28, 103, 264, 329, 351
BstMAI GTCTC 1 cut(s) 494
BstMBI GATC 5 cut(s) 25, 100, 261, 326, 348
BstMWI GCNNNNNNNGC 2 cut(s) 181, 588
BstNSI RCATGY 1 cut(s) 727
BstV1I GCAGC 5 cut(s) 187, 190, 193, 572, 594
BstV2I GAAGAC 1 cut(s) 298
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 2 cut(s) 389, 645
BtsCI GGATG 1 cut(s) 143
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 2 cut(s) 567, 710
Cac8I GCNNGC 2 cut(s) 173, 636
CciI TCATGA 2 cut(s) 22, 115
Csp6I GTAC 2 cut(s) 95, 526
CviAII CATG 5 cut(s) 23, 116, 379, 535, 724
CviJI RGCY 4 cut(s) 298, 389, 585, 645
CviKI_1 RGCY 4 cut(s) 298, 389, 585, 645
CviQI GTAC 2 cut(s) 95, 526
DdeI CTNAG 1 cut(s) 647
DpnI GATC 5 cut(s) 27, 102, 263, 328, 350
DpnII GATC 5 cut(s) 25, 100, 261, 326, 348
Eco147I AGGCCT 1 cut(s) 645
Eco31I GGTCTC 1 cut(s) 494
Eco57I CTGAAG 1 cut(s) 660
EcoRI GAATTC 1 cut(s) 52
FaeI CATG 5 cut(s) 26, 119, 382, 538, 727
FalI AAGNNNNNCTT 2 cut(s) 618, 650
FaqI GGGAC 1 cut(s) 287
FatI CATG 5 cut(s) 22, 115, 378, 534, 723
FauI CCCGC 1 cut(s) 102
FauNDI CATATG 1 cut(s) 195
FbaI TGATCA 2 cut(s) 25, 348
Fnu4HI GCNGC 6 cut(s) 176, 179, 182, 583, 586, 589
FokI GGATG 1 cut(s) 130
Fsp4HI GCNGC 6 cut(s) 176, 179, 182, 583, 586, 589
GluI GCNGC 6 cut(s) 176, 179, 182, 583, 586, 589
GsuI CTGGAG 1 cut(s) 246
HaeIII GGCC 2 cut(s) 389, 645
Hin1II CATG 5 cut(s) 26, 119, 382, 538, 727
HinfI GANTC 3 cut(s) 68, 139, 473
HphI GGTGA 2 cut(s) 198, 361
Hpy188I TCNGA 1 cut(s) 740
Hpy188III TCNNGA 8 cut(s) 23, 49, 116, 128, 491, 512, 615, 729
HpyAV CCTTC 4 cut(s) 406, 436, 593, 635
HpyCH4IV ACGT 1 cut(s) 361
HpyCH4V TGCA 1 cut(s) 91
HpyF10VI GCNNNNNNNGC 2 cut(s) 181, 588
HpyF3I CTNAG 1 cut(s) 647
HpySE526I ACGT 1 cut(s) 361
Hsp92II CATG 5 cut(s) 26, 119, 382, 538, 727
Ksp22I TGATCA 2 cut(s) 25, 348
Kzo9I GATC 5 cut(s) 25, 100, 261, 326, 348
LpnPI CCDG 8 cut(s) 62, 141, 210, 229, 352, 562, 685, 714
Lsp1109I GCAGC 5 cut(s) 187, 190, 193, 572, 594
LweI GCATC 1 cut(s) 78
MaeII ACGT 1 cut(s) 361
MaeIII GTNAC 1 cut(s) 509
MalI GATC 5 cut(s) 27, 102, 263, 328, 350
MboI GATC 5 cut(s) 25, 100, 261, 326, 348
MboII GAAGA 5 cut(s) 148, 271, 303, 430, 437
MflI RGATCY 1 cut(s) 100
MhlI GDGCHC 2 cut(s) 378, 624
MluCI AATT 6 cut(s) 10, 52, 211, 313, 344, 394
MnlI CCTC 9 cut(s) 112, 124, 341, 344, 379, 559, 586, 589, 618
MroXI GAANNNNTTC 1 cut(s) 361
MseI TTAA 3 cut(s) 312, 453, 522
MslI CAYNNNNRTG 2 cut(s) 78, 381
MspA1I CMGCKG 1 cut(s) 585
MspCI CTTAAG 1 cut(s) 521
Mva1269I GAATGC 1 cut(s) 439
MwoI GCNNNNNNNGC 2 cut(s) 181, 588
NdeI CATATG 1 cut(s) 195
NdeII GATC 5 cut(s) 25, 100, 261, 326, 348
NlaIII CATG 5 cut(s) 26, 119, 382, 538, 727
NmuCI GTSAC 1 cut(s) 509
NspI RCATGY 1 cut(s) 727
PagI TCATGA 2 cut(s) 22, 115
PceI AGGCCT 1 cut(s) 645
PctI GAATGC 1 cut(s) 439
PdmI GAANNNNTTC 1 cut(s) 361
PfeI GAWTC 3 cut(s) 68, 139, 473
PkrI GCNGC 6 cut(s) 177, 180, 183, 584, 587, 590
Psp1406I AACGTT 1 cut(s) 361
PsuI RGATCY 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 585
RsaI GTAC 2 cut(s) 96, 527
RsaNI GTAC 2 cut(s) 95, 526
RseI CAYNNNNRTG 2 cut(s) 78, 381
SaqAI TTAA 3 cut(s) 312, 453, 522
SatI GCNGC 6 cut(s) 176, 179, 182, 583, 586, 589
Sau3AI GATC 5 cut(s) 25, 100, 261, 326, 348
ScaI AGTACT 1 cut(s) 527
SduI GDGCHC 2 cut(s) 378, 624
SetI ASST 7 cut(s) 123, 211, 300, 364, 551, 570, 587
SfaNI GCATC 1 cut(s) 78
SmiMI CAYNNNNRTG 2 cut(s) 78, 381
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 6 cut(s) 10, 52, 211, 313, 344, 394
SseBI AGGCCT 1 cut(s) 645
SsiI CCGC 2 cut(s) 109, 588
StuI AGGCCT 1 cut(s) 645
TaiI ACGT 1 cut(s) 364
TaqI TCGA 2 cut(s) 325, 492
TasI AATT 6 cut(s) 10, 52, 211, 313, 344, 394
TatI WGTACW 2 cut(s) 94, 525
TauI GCSGC 1 cut(s) 591
TfiI GAWTC 3 cut(s) 68, 139, 473
Tru1I TTAA 3 cut(s) 312, 453, 522
Tru9I TTAA 3 cut(s) 312, 453, 522
TscAI CASTG 2 cut(s) 567, 710
TseFI GTSAC 1 cut(s) 509
TseI GCWGC 5 cut(s) 175, 178, 181, 582, 585
Tsp45I GTSAC 1 cut(s) 509
TspDTI ATGAA 5 cut(s) 306, 395, 480, 513, 696
TspRI CASTG 2 cut(s) 567, 710
Vha464I CTTAAG 1 cut(s) 521
XapI RAATTY 1 cut(s) 52
XceI RCATGY 1 cut(s) 727
XmnI GAANNNNTTC 1 cut(s) 361
ZrmI AGTACT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.