MD01G1191700.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006418
Physical Location & Seq
Forward (+)
29029081 .. 29030338
1258 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1191700.v1.1.491

Sequence Viewer

Length: 792 bp
ATGTTTCCCAATGCATATCATCGTGGTCAAAGCAAAGATTTGGTATTCCAGACCTCCACTATCAATTATCCCCACCGAGAAGATATGATGCGTGTACAAGATCTTATGCGGTGTCATGAGGTGTTTCCAGAGGGGAAGAATCCATCCCGTAATAAGATGATGGGGAAAGGCAAGCAACAACAACAACAGCAACATCGTATGATATTATCACCTAATTCCAACAATAATGGAGCAAATCCTAGTGATGGTAAGACTGAAAGGAAGATCGTGCGTAGGGACAACGAACGCCAAAGAAGACAACTTATGGCAGCTCTTAATGCATCACTTCGATCCCTCCTCCCCTATGAATTGATCAAGGGAAAACGTTCAATCACCGAGCACATGAATGAGGCCGTCAACTATATAAATCACATGAAGGCAAAGATCGAAGAACTGAATGCCAAGAAGGAAAAGATTAATAAAAAGTTGTATGAATGTGATTCTCAAGATTTTGGTCTCAGAAATGAAGGTTCGGGTCACAATTTTCTCAAGTACTCTATCATGGTTCGCCCAACTTGTTTGGGAGGAGTGGAGGTTTTGGTAAGTAGTAGCTGCGGTGAGGAGGAAGGGTTGCTTCTTTCGGGAGTGCTCAAAGTATTGCTTGCGAAAGGCCTTAGTGTTGTTGAGTGTGCTTCCACCAGAGTAAATGAAAGTTTGTTTCACACCATTCAGTGTGAGGTTGTCAACGATTTGGCATGTCTGGATTTGTCTGAGTTGCAACTGAAACTAAATAACCATTACAATTCCGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

264

Amino Acids

29.89

Weight (kDa)

9.01

Isoelectric Point (pI)

58.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 87 - 138 1e-05 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 109, 594
AclI AACGTT 1 cut(s) 364
AclWI GGATC 1 cut(s) 324
AfaI GTAC 2 cut(s) 96, 533
AfiI CCNNNNNNNGG 1 cut(s) 245
AgsI TTSAA 1 cut(s) 369
AluBI AGCT 2 cut(s) 311, 591
AluI AGCT 2 cut(s) 311, 591
Alw21I GWGCWC 2 cut(s) 381, 630
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 1 cut(s) 324
AoxI GGCC 2 cut(s) 390, 649
ApeKI GCWGC 2 cut(s) 308, 591
AseI ATTAAT 1 cut(s) 456
Asp700I GAANNNNTTC 1 cut(s) 364
AsuC2I CCSGG 1 cut(s) 787
AsuHPI GGTGA 3 cut(s) 201, 364, 608
BbsI GAAGAC 1 cut(s) 301
Bbv12I GWGCWC 2 cut(s) 381, 630
BbvI GCAGC 2 cut(s) 320, 578
BccI CCATC 3 cut(s) 151, 154, 239
BceAI ACGGC 1 cut(s) 377
BclI TGATCA 1 cut(s) 351
BcnI CCSGG 1 cut(s) 787
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 1 cut(s) 240
BglII AGATCT 1 cut(s) 100
BisI GCNGC 2 cut(s) 309, 592
BlsI GCNGC 2 cut(s) 310, 593
BmcAI AGTACT 1 cut(s) 533
Bme1390I CCNGG 1 cut(s) 787
BmrFI CCNGG 1 cut(s) 787
BmsI GCATC 2 cut(s) 78, 329
BpiI GAAGAC 1 cut(s) 301
BpuEI CTTGAG 2 cut(s) 468, 512
BpuMI CCSGG 1 cut(s) 787
BsaI GGTCTC 1 cut(s) 500
BsaXI ACNNNNNCTCC 4 cut(s) 558, 588, 593, 623
Bsc4I CCNNNNNNNGG 1 cut(s) 245
BseGI GGATG 1 cut(s) 143
BseLI CCNNNNNNNGG 1 cut(s) 245
BseMII CTCAG 2 cut(s) 511, 741
BseRI GAGGAG 3 cut(s) 326, 579, 614
BseXI GCAGC 2 cut(s) 320, 578
BshFI GGCC 2 cut(s) 392, 651
BsiHKAI GWGCWC 2 cut(s) 381, 630
BsiSI CCGG 1 cut(s) 786
BslFI GGGAC 1 cut(s) 290
BslI CCNNNNNNNGG 1 cut(s) 245
BsmAI GTCTC 1 cut(s) 500
BsmFI GGGAC 1 cut(s) 290
BsmI GAATGC 1 cut(s) 442
BsnI GGCC 2 cut(s) 392, 651
Bso31I GGTCTC 1 cut(s) 500
Bsp1286I GDGCHC 2 cut(s) 381, 630
Bsp1407I TGTACA 1 cut(s) 94
Bsp143I GATC 5 cut(s) 100, 264, 329, 351, 423
BspACI CCGC 2 cut(s) 109, 594
BspANI GGCC 2 cut(s) 392, 651
BspCNI CTCAG 2 cut(s) 510, 742
BspHI TCATGA 1 cut(s) 115
BspPI GGATC 1 cut(s) 324
BspTNI GGTCTC 1 cut(s) 500
BsrGI TGTACA 1 cut(s) 94
BssMI GATC 5 cut(s) 100, 264, 329, 351, 423
BstAUI TGTACA 1 cut(s) 94
BstC8I GCNNGC 2 cut(s) 173, 642
BstDEI CTNAG 3 cut(s) 497, 653, 750
BstF5I GGATG 1 cut(s) 143
BstKTI GATC 5 cut(s) 103, 267, 332, 354, 426
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 5 cut(s) 100, 264, 329, 351, 423
BstMWI GCNNNNNNNGC 1 cut(s) 317
BstNSI RCATGY 1 cut(s) 738
BstSCI CCNGG 1 cut(s) 785
BstV1I GCAGC 2 cut(s) 320, 578
BstV2I GAAGAC 1 cut(s) 301
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 2 cut(s) 392, 651
BtsCI GGATG 1 cut(s) 143
BtsIMutI CAGTG 1 cut(s) 716
Cac8I GCNNGC 2 cut(s) 173, 642
CciI TCATGA 1 cut(s) 115
Csp6I GTAC 2 cut(s) 95, 532
CviAII CATG 5 cut(s) 116, 382, 412, 541, 735
CviJI RGCY 4 cut(s) 311, 392, 591, 651
CviKI_1 RGCY 4 cut(s) 311, 392, 591, 651
CviQI GTAC 2 cut(s) 95, 532
DdeI CTNAG 3 cut(s) 497, 653, 750
DpnI GATC 5 cut(s) 102, 266, 331, 353, 425
DpnII GATC 5 cut(s) 100, 264, 329, 351, 423
Eco147I AGGCCT 1 cut(s) 651
Eco31I GGTCTC 1 cut(s) 500
EcoT22I ATGCAT 2 cut(s) 16, 322
FaeI CATG 5 cut(s) 119, 385, 415, 544, 738
FalI AAGNNNNNCTT 4 cut(s) 597, 629, 624, 656
FaqI GGGAC 1 cut(s) 290
FatI CATG 5 cut(s) 115, 381, 411, 540, 734
FbaI TGATCA 1 cut(s) 351
Fnu4HI GCNGC 2 cut(s) 309, 592
FokI GGATG 1 cut(s) 130
Fsp4HI GCNGC 2 cut(s) 309, 592
FspBI CTAG 1 cut(s) 240
GluI GCNGC 2 cut(s) 309, 592
HaeIII GGCC 2 cut(s) 392, 651
HapII CCGG 1 cut(s) 786
Hin1II CATG 5 cut(s) 119, 385, 415, 544, 738
HincII GTYRAC 2 cut(s) 397, 724
HindII GTYRAC 2 cut(s) 397, 724
HinfI GANTC 2 cut(s) 139, 479
HpaII CCGG 1 cut(s) 786
HphI GGTGA 3 cut(s) 201, 364, 608
Hpy166II GTNNAC 3 cut(s) 95, 397, 724
Hpy188I TCNGA 2 cut(s) 500, 751
Hpy188III TCNNGA 6 cut(s) 49, 116, 128, 485, 621, 740
Hpy8I GTNNAC 3 cut(s) 95, 397, 724
HpyAV CCTTC 4 cut(s) 409, 439, 500, 599
HpyCH4IV ACGT 1 cut(s) 364
HpyCH4V TGCA 3 cut(s) 14, 320, 757
HpyF10VI GCNNNNNNNGC 1 cut(s) 317
HpyF3I CTNAG 3 cut(s) 497, 653, 750
HpySE526I ACGT 1 cut(s) 364
Hsp92II CATG 5 cut(s) 119, 385, 415, 544, 738
Ksp22I TGATCA 1 cut(s) 351
Kzo9I GATC 5 cut(s) 100, 264, 329, 351, 423
LmnI GCTCC 1 cut(s) 230
LpnPI CCDG 4 cut(s) 62, 141, 691, 725
Lsp1109I GCAGC 2 cut(s) 320, 578
LweI GCATC 2 cut(s) 78, 329
MaeI CTAG 1 cut(s) 240
MaeII ACGT 1 cut(s) 364
MaeIII GTNAC 1 cut(s) 515
MalI GATC 5 cut(s) 102, 266, 331, 353, 425
MboI GATC 5 cut(s) 100, 264, 329, 351, 423
MboII GAAGA 5 cut(s) 92, 148, 274, 306, 440
MflI RGATCY 1 cut(s) 100
MhlI GDGCHC 2 cut(s) 381, 630
MluCI AATT 5 cut(s) 64, 214, 347, 520, 781
MmeI TCCRAC 1 cut(s) 243
Mph1103I ATGCAT 2 cut(s) 16, 322
MroXI GAANNNNTTC 1 cut(s) 364
MseI TTAA 2 cut(s) 315, 456
MslI CAYNNNNRTG 1 cut(s) 384
MspI CCGG 1 cut(s) 786
MspR9I CCNGG 1 cut(s) 787
Mva1269I GAATGC 1 cut(s) 442
MwoI GCNNNNNNNGC 1 cut(s) 317
NciI CCSGG 1 cut(s) 787
NdeII GATC 5 cut(s) 100, 264, 329, 351, 423
NlaIII CATG 5 cut(s) 119, 385, 415, 544, 738
NmuCI GTSAC 1 cut(s) 515
NsiI ATGCAT 2 cut(s) 16, 322
NspI RCATGY 1 cut(s) 738
PagI TCATGA 1 cut(s) 115
PceI AGGCCT 1 cut(s) 651
PctI GAATGC 1 cut(s) 442
PdmI GAANNNNTTC 1 cut(s) 364
PfeI GAWTC 2 cut(s) 139, 479
PkrI GCNGC 2 cut(s) 310, 593
PshBI ATTAAT 1 cut(s) 456
Psp1406I AACGTT 1 cut(s) 364
PsuI RGATCY 1 cut(s) 100
RsaI GTAC 2 cut(s) 96, 533
RsaNI GTAC 2 cut(s) 95, 532
RseI CAYNNNNRTG 1 cut(s) 384
SaqAI TTAA 2 cut(s) 315, 456
SatI GCNGC 2 cut(s) 309, 592
Sau3AI GATC 5 cut(s) 100, 264, 329, 351, 423
ScaI AGTACT 1 cut(s) 533
ScrFI CCNGG 1 cut(s) 787
SduI GDGCHC 2 cut(s) 381, 630
SetI ASST 9 cut(s) 56, 123, 214, 313, 367, 511, 576, 593, 720
SfaNI GCATC 2 cut(s) 78, 329
SmiMI CAYNNNNRTG 1 cut(s) 384
SmlI CTYRAG 2 cut(s) 483, 527
SmoI CTYRAG 2 cut(s) 483, 527
Sse9I AATT 5 cut(s) 64, 214, 347, 520, 781
SseBI AGGCCT 1 cut(s) 651
SsiI CCGC 2 cut(s) 109, 594
SspMI CTAG 1 cut(s) 240
StuI AGGCCT 1 cut(s) 651
StyD4I CCNGG 1 cut(s) 785
TaiI ACGT 1 cut(s) 367
TaqI TCGA 2 cut(s) 328, 426
TasI AATT 5 cut(s) 64, 214, 347, 520, 781
TatI WGTACW 2 cut(s) 94, 531
TfiI GAWTC 2 cut(s) 139, 479
Tru1I TTAA 2 cut(s) 315, 456
Tru9I TTAA 2 cut(s) 315, 456
TscAI CASTG 1 cut(s) 716
TseFI GTSAC 1 cut(s) 515
TseI GCWGC 2 cut(s) 308, 591
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 6 cut(s) 360, 398, 428, 486, 519, 702
TspRI CASTG 1 cut(s) 716
VspI ATTAAT 1 cut(s) 456
XceI RCATGY 1 cut(s) 738
XmnI GAANNNNTTC 1 cut(s) 364
XspI CTAG 1 cut(s) 240
ZrmI AGTACT 1 cut(s) 533
Zsp2I ATGCAT 2 cut(s) 16, 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.