Rw1G035950

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006418
Physical Location & Seq
Forward (+)
63763389 .. 63764396
1008 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035950.1

Sequence Viewer

Length: 753 bp
ATGTCTCCTTTCCCTCATGATCATCAAAGCAAAGATATGGAATTACAAATATCTTTGACTCCCCACCAAGAAGATTTTATGCAACAGGATCTGATCATGATGCGGGGTAGTCATGAGGTTTTGCCATCTGATCTGCAGGGACGTAGTCTATCCAATATTAATATTAGTGAAGCAAATATTAACAATATGGCTGCTGATTCTGACGAAAGGAAGATCATGCGTAGGGACAACGAGCGCCAAAGAAGACAAGTTATGGCGATTCGCACTGCATCACTTCGATCCCTCCTCCCTATAGAATTAATCAAGGGAAAACGTTCAATCTCCGAGCACATAAATGAAGCAGTGAATTATATAAAGCAGCTGAAGGAAAAGATACAGGAGCTGAATGCCAAGAGGGGAGAGCTTAGAAGGGTGTATAAACATTACAGTAGTTCTGCACATGATTTCAGTCCTGAAATTAATGAAAATTTGGGTACTAAGAGTACTTCCAATATCATGGTTGGCCCAACATGTTGGGGTGGAGTGGAGATTGTGGTAAGTACTAATGGTACAAATTGCAGTGAGCTAGAAGGGTTTTCTCTTTCAGGAGTACTCAAAGCATTGCTTGCAGAGGGCCTTACCGTTGTGGAGTGTGCTTCCACCACAGCAAATCAGAGGTTGATTCACTCTATTCAGTGTGAGGTCGACGATTTGGCATGTCTGGATTTGTCTGAGCTACAACTGAAACTAAATTTTTTATGCAATTCTGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

250

Amino Acids

28.01

Weight (kDa)

5.78

Isoelectric Point (pI)

61.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 70 - 122 1.1e-06 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 684
AciI CCGC 1 cut(s) 103
AclI AACGTT 1 cut(s) 313
AclWI GGATC 2 cut(s) 96, 273
AcsI RAATTY 2 cut(s) 466, 730
AcuI CTGAAG 1 cut(s) 383
AfaI GTAC 5 cut(s) 475, 484, 541, 550, 591
AflIII ACRYGT 1 cut(s) 509
AgsI TTSAA 1 cut(s) 318
AluBI AGCT 5 cut(s) 361, 382, 403, 565, 715
AluI AGCT 5 cut(s) 361, 382, 403, 565, 715
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 2 cut(s) 96, 273
AlwNI CAGNNNCTG 2 cut(s) 91, 382
AoxI GGCC 2 cut(s) 502, 613
ApeKI GCWGC 2 cut(s) 191, 358
ApoI RAATTY 2 cut(s) 466, 730
AseI ATTAAT 3 cut(s) 159, 299, 459
Asp700I GAANNNNTTC 1 cut(s) 313
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 2 cut(s) 503, 613
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 330
BbvI GCAGC 2 cut(s) 178, 370
BccI CCATC 1 cut(s) 133
BclI TGATCA 2 cut(s) 19, 93
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 2 cut(s) 566, 751
BfmI CTRYAG 2 cut(s) 134, 291
BfoI RGCGCY 1 cut(s) 238
BisI GCNGC 2 cut(s) 192, 359
BlsI GCNGC 2 cut(s) 193, 360
BmcAI AGTACT 3 cut(s) 484, 541, 591
BmgT120I GGNCC 2 cut(s) 503, 613
BmsI GCATC 2 cut(s) 90, 278
BpiI GAAGAC 1 cut(s) 250
Bse3DI GCAATG 1 cut(s) 599
BseMI GCAATG 1 cut(s) 599
BseMII CTCAG 1 cut(s) 702
BseRI GAGGAG 1 cut(s) 275
BseXI GCAGC 2 cut(s) 178, 370
BsgI GTGCAG 1 cut(s) 420
BshFI GGCC 2 cut(s) 504, 615
BsiHKAI GWGCWC 1 cut(s) 330
BslFI GGGAC 2 cut(s) 153, 239
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 2 cut(s) 153, 239
BsmI GAATGC 1 cut(s) 391
BsnI GGCC 2 cut(s) 504, 615
Bsp1286I GDGCHC 1 cut(s) 330
Bsp143I GATC 6 cut(s) 19, 88, 93, 130, 213, 278
BspACI CCGC 1 cut(s) 103
BspANI GGCC 2 cut(s) 504, 615
BspCNI CTCAG 1 cut(s) 703
BspHI TCATGA 3 cut(s) 16, 96, 112
BspMAI CTGCAG 1 cut(s) 138
BspPI GGATC 2 cut(s) 96, 273
BsrDI GCAATG 1 cut(s) 599
BssMI GATC 6 cut(s) 19, 88, 93, 130, 213, 278
Bst4CI ACNGT 2 cut(s) 428, 622
BstAPI GCANNNNNTGC 1 cut(s) 605
BstC8I GCNNGC 1 cut(s) 606
BstDEI CTNAG 3 cut(s) 404, 477, 711
BstH2I RGCGCY 1 cut(s) 238
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 6 cut(s) 22, 91, 96, 133, 216, 281
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 6 cut(s) 19, 88, 93, 130, 213, 278
BstMWI GCNNNNNNNGC 2 cut(s) 605, 747
BstNSI RCATGY 2 cut(s) 513, 699
BstSFI CTRYAG 2 cut(s) 134, 291
BstV1I GCAGC 2 cut(s) 178, 370
BstV2I GAAGAC 1 cut(s) 250
BstX2I RGATCY 1 cut(s) 88
BstXI CCANNNNNNTGG 2 cut(s) 496, 513
BstYI RGATCY 1 cut(s) 88
BsuRI GGCC 2 cut(s) 504, 615
BtsI GCAGTG 3 cut(s) 264, 348, 565
BtsIMutI CAGTG 4 cut(s) 264, 348, 565, 680
Cac8I GCNNGC 1 cut(s) 606
CaiI CAGNNNCTG 2 cut(s) 91, 382
CciI TCATGA 3 cut(s) 16, 96, 112
CfoI GCGC 1 cut(s) 237
Cfr13I GGNCC 2 cut(s) 503, 613
Csp6I GTAC 5 cut(s) 474, 483, 540, 549, 590
CviAII CATG 8 cut(s) 17, 97, 113, 217, 440, 496, 510, 696
CviJI RGCY 9 cut(s) 191, 361, 382, 403, 504, 565, 615, 715, 750
CviKI_1 RGCY 9 cut(s) 191, 361, 382, 403, 504, 565, 615, 715, 750
CviQI GTAC 5 cut(s) 474, 483, 540, 549, 590
DdeI CTNAG 3 cut(s) 404, 477, 711
DpnI GATC 6 cut(s) 21, 90, 95, 132, 215, 280
DpnII GATC 6 cut(s) 19, 88, 93, 130, 213, 278
Eco57I CTGAAG 1 cut(s) 383
EcoO109I RGGNCCY 1 cut(s) 613
FaeI CATG 8 cut(s) 20, 100, 116, 220, 443, 499, 513, 699
FalI AAGNNNNNCTT 2 cut(s) 588, 620
FaqI GGGAC 2 cut(s) 153, 239
FatI CATG 8 cut(s) 16, 96, 112, 216, 439, 495, 509, 695
FauI CCCGC 1 cut(s) 96
FbaI TGATCA 2 cut(s) 19, 93
FblI GTMKAC 1 cut(s) 684
Fnu4HI GCNGC 2 cut(s) 192, 359
Fsp4HI GCNGC 2 cut(s) 192, 359
FspBI CTAG 2 cut(s) 566, 751
GlaI GCGC 1 cut(s) 236
GluI GCNGC 2 cut(s) 192, 359
HaeII RGCGCY 1 cut(s) 238
HaeIII GGCC 2 cut(s) 504, 615
HhaI GCGC 1 cut(s) 237
Hin1II CATG 8 cut(s) 20, 100, 116, 220, 443, 499, 513, 699
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HincII GTYRAC 1 cut(s) 685
HindII GTYRAC 1 cut(s) 685
HinfI GANTC 4 cut(s) 58, 197, 259, 661
Hpy166II GTNNAC 1 cut(s) 685
Hpy188I TCNGA 6 cut(s) 93, 130, 202, 325, 654, 712
Hpy188III TCNNGA 6 cut(s) 17, 97, 113, 452, 585, 701
Hpy8I GTNNAC 1 cut(s) 685
Hpy99I CGWCG 1 cut(s) 689
HpyAV CCTTC 3 cut(s) 358, 402, 563
HpyCH4III ACNGT 2 cut(s) 428, 622
HpyCH4IV ACGT 2 cut(s) 142, 313
HpyCH4V TGCA 7 cut(s) 82, 136, 269, 437, 558, 608, 741
HpyF10VI GCNNNNNNNGC 2 cut(s) 605, 747
HpyF3I CTNAG 3 cut(s) 404, 477, 711
HpySE526I ACGT 2 cut(s) 142, 313
Hsp92II CATG 8 cut(s) 20, 100, 116, 220, 443, 499, 513, 699
HspAI GCGC 1 cut(s) 235
Ksp22I TGATCA 2 cut(s) 19, 93
Kzo9I GATC 6 cut(s) 19, 88, 93, 130, 213, 278
LmnI GCTCC 1 cut(s) 379
LpnPI CCDG 7 cut(s) 71, 122, 362, 465, 570, 686, 732
Lsp1109I GCAGC 2 cut(s) 178, 370
LweI GCATC 2 cut(s) 90, 278
MaeI CTAG 2 cut(s) 566, 751
MaeII ACGT 2 cut(s) 142, 313
MalI GATC 6 cut(s) 21, 90, 95, 132, 215, 280
MboI GATC 6 cut(s) 19, 88, 93, 130, 213, 278
MboII GAAGA 3 cut(s) 83, 223, 255
MflI RGATCY 1 cut(s) 88
MhlI GDGCHC 1 cut(s) 330
MluCI AATT 8 cut(s) 41, 296, 346, 456, 466, 553, 730, 742
MlyI GAGTC 1 cut(s) 52
MnlI CCTC 8 cut(s) 24, 109, 293, 296, 387, 604, 648, 673
MroXI GAANNNNTTC 1 cut(s) 313
MseI TTAA 4 cut(s) 159, 180, 299, 459
MslI CAYNNNNRTG 1 cut(s) 333
MspA1I CMGCKG 1 cut(s) 361
Mva1269I GAATGC 1 cut(s) 391
MwoI GCNNNNNNNGC 2 cut(s) 605, 747
NdeII GATC 6 cut(s) 19, 88, 93, 130, 213, 278
NlaIII CATG 8 cut(s) 20, 100, 116, 220, 443, 499, 513, 699
NspI RCATGY 2 cut(s) 513, 699
PagI TCATGA 3 cut(s) 16, 96, 112
PciI ACATGT 1 cut(s) 509
PctI GAATGC 1 cut(s) 391
PdmI GAANNNNTTC 1 cut(s) 313
PfeI GAWTC 3 cut(s) 197, 259, 661
PflFI GACNNNGTC 1 cut(s) 144
PkrI GCNGC 2 cut(s) 193, 360
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
PscI ACATGT 1 cut(s) 509
PshBI ATTAAT 3 cut(s) 159, 299, 459
Psp1406I AACGTT 1 cut(s) 313
PspPI GGNCC 2 cut(s) 503, 613
PstI CTGCAG 1 cut(s) 138
PstNI CAGNNNCTG 2 cut(s) 91, 382
PsuI RGATCY 1 cut(s) 88
PsyI GACNNNGTC 1 cut(s) 144
PvuII CAGCTG 1 cut(s) 361
RsaI GTAC 5 cut(s) 475, 484, 541, 550, 591
RsaNI GTAC 5 cut(s) 474, 483, 540, 549, 590
RseI CAYNNNNRTG 1 cut(s) 333
SalI GTCGAC 1 cut(s) 683
SaqAI TTAA 4 cut(s) 159, 180, 299, 459
SatI GCNGC 2 cut(s) 192, 359
Sau3AI GATC 6 cut(s) 19, 88, 93, 130, 213, 278
Sau96I GGNCC 2 cut(s) 503, 613
ScaI AGTACT 3 cut(s) 484, 541, 591
SchI GAGTC 1 cut(s) 52
SduI GDGCHC 1 cut(s) 330
SfaNI GCATC 2 cut(s) 90, 278
SfcI CTRYAG 2 cut(s) 134, 291
SmiMI CAYNNNNRTG 1 cut(s) 333
Sse9I AATT 8 cut(s) 41, 296, 346, 456, 466, 553, 730, 742
SsiI CCGC 1 cut(s) 103
SspI AATATT 3 cut(s) 157, 163, 178
SspMI CTAG 2 cut(s) 566, 751
TaaI ACNGT 2 cut(s) 428, 622
TaiI ACGT 2 cut(s) 145, 316
TaqI TCGA 2 cut(s) 277, 684
TasI AATT 8 cut(s) 41, 296, 346, 456, 466, 553, 730, 742
TatI WGTACW 3 cut(s) 482, 539, 589
TfiI GAWTC 3 cut(s) 197, 259, 661
Tru1I TTAA 4 cut(s) 159, 180, 299, 459
Tru9I TTAA 4 cut(s) 159, 180, 299, 459
TscAI CASTG 4 cut(s) 271, 348, 565, 680
TseI GCWGC 2 cut(s) 191, 358
TspDTI ATGAA 2 cut(s) 351, 477
TspRI CASTG 4 cut(s) 271, 348, 565, 680
Tth111I GACNNNGTC 1 cut(s) 144
VspI ATTAAT 3 cut(s) 159, 299, 459
XapI RAATTY 2 cut(s) 466, 730
XceI RCATGY 2 cut(s) 513, 699
XmiI GTMKAC 1 cut(s) 684
XmnI GAANNNNTTC 1 cut(s) 313
XspI CTAG 2 cut(s) 566, 751
ZrmI AGTACT 3 cut(s) 484, 541, 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.