pycom01g20290

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0006418
Physical Location & Seq
Forward (+)
18661888 .. 18663036
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g20290.1

Sequence Viewer

Length: 789 bp
ATGTTTCCCAATTCATATCATCGTGGTCAAAGCAAAGATTTGGTATTCCAGACCTCCACTATCAATTTTCCCCACCGGGAAGATATGATGCATGTACAAGATCTGATGCGGGGTCATGAGGTGTTTCCAGAGGGGAAGAATCCATCCCGTAATAAGATGATGGGGAAAGGCAAGCAACAACAACAGCAACATCGTATGATATTATCACCTAATTCCAACAATAATGGAGCAAATCCTAGTGATGGTAAGACTGAAAGGAAGATCGTGCGTAGGGACAACGAACGCCAAAGACGACAACATATGGCAGCTCTTCATGCATCACTTCGATCCCTCCTCCCCTATGAATTGATCAAGGGAAAACGTTCAATCACTGAGCACATGAATGAGGCCGTCAACTATATAAATCACTTGAAGGCGAAGATCGAAGAACTGAATGCCAAGAAGGAAAAGATTAATAAAAAGTTGTATGAATGTGATTCTCAAGATTTTGGTCTCAGAAATGAAAGTTCGGGTCACAATTTTCTCAAGTACTCTATCATGGTTCGCCCAACTTGTTTGGGAGGAGTGGAGGTTTTGGTAAGTAGTAGCTGCGGTGAGGAGGAAGGGTTGCTTCTTTCAGGAGTGCTCAAAGTATTGCTTGCGGAAGGCCTTAGTGTTGTTGAGTATGCTTCCACCAGAGTAAATGAAAGTTTGTTTCACACCATTCAGTGTGAGGTTGTCAACGATTTGGCATGTCTGGATTTGTCTGAGTTGCAACTGAAACTAAATAACTATTACAATTCCGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

263

Amino Acids

29.84

Weight (kDa)

8.71

Isoelectric Point (pI)

57.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 86 - 138 1.9e-07 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 109, 591, 641
AclI AACGTT 1 cut(s) 361
AclWI GGATC 1 cut(s) 321
AfaI GTAC 2 cut(s) 96, 530
AfiI CCNNNNNNNGG 1 cut(s) 242
AgsI TTSAA 2 cut(s) 366, 412
AluBI AGCT 2 cut(s) 308, 588
AluI AGCT 2 cut(s) 308, 588
Alw21I GWGCWC 2 cut(s) 378, 627
Alw26I GTCTC 1 cut(s) 497
AlwI GGATC 1 cut(s) 321
AoxI GGCC 2 cut(s) 387, 646
ApeKI GCWGC 2 cut(s) 305, 588
AseI ATTAAT 1 cut(s) 453
Asp700I GAANNNNTTC 1 cut(s) 361
AsuC2I CCSGG 2 cut(s) 77, 784
AsuHPI GGTGA 2 cut(s) 198, 605
Bbv12I GWGCWC 2 cut(s) 378, 627
BbvI GCAGC 2 cut(s) 317, 575
BccI CCATC 3 cut(s) 151, 154, 236
BceAI ACGGC 1 cut(s) 374
BclI TGATCA 1 cut(s) 348
BcnI CCSGG 2 cut(s) 77, 784
BcoDI GTCTC 1 cut(s) 497
BfaI CTAG 1 cut(s) 237
BglII AGATCT 1 cut(s) 100
BisI GCNGC 2 cut(s) 306, 589
BlsI GCNGC 2 cut(s) 307, 590
BmcAI AGTACT 1 cut(s) 530
Bme1390I CCNGG 2 cut(s) 77, 784
BmrFI CCNGG 2 cut(s) 77, 784
BmsI GCATC 3 cut(s) 78, 96, 326
BpuEI CTTGAG 2 cut(s) 465, 509
BpuMI CCSGG 2 cut(s) 77, 784
BsaI GGTCTC 1 cut(s) 497
BsaXI ACNNNNNCTCC 4 cut(s) 555, 585, 590, 620
Bsc4I CCNNNNNNNGG 1 cut(s) 242
BseGI GGATG 1 cut(s) 143
BseLI CCNNNNNNNGG 1 cut(s) 242
BseMII CTCAG 3 cut(s) 363, 508, 738
BseRI GAGGAG 3 cut(s) 323, 576, 611
BseXI GCAGC 2 cut(s) 317, 575
BshFI GGCC 2 cut(s) 389, 648
BsiHKAI GWGCWC 2 cut(s) 378, 627
BsiSI CCGG 2 cut(s) 76, 783
BslFI GGGAC 1 cut(s) 287
BslI CCNNNNNNNGG 1 cut(s) 242
BsmAI GTCTC 1 cut(s) 497
BsmFI GGGAC 1 cut(s) 287
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 2 cut(s) 389, 648
Bso31I GGTCTC 1 cut(s) 497
Bsp1286I GDGCHC 2 cut(s) 378, 627
Bsp1407I TGTACA 1 cut(s) 94
Bsp143I GATC 5 cut(s) 100, 261, 326, 348, 420
BspACI CCGC 3 cut(s) 109, 591, 641
BspANI GGCC 2 cut(s) 389, 648
BspCNI CTCAG 3 cut(s) 364, 507, 739
BspHI TCATGA 1 cut(s) 115
BspPI GGATC 1 cut(s) 321
BspQI GCTCTTC 1 cut(s) 315
BspTNI GGTCTC 1 cut(s) 497
BsrGI TGTACA 1 cut(s) 94
BssMI GATC 5 cut(s) 100, 261, 326, 348, 420
Bst6I CTCTTC 1 cut(s) 315
BstAUI TGTACA 1 cut(s) 94
BstC8I GCNNGC 2 cut(s) 173, 639
BstDEI CTNAG 4 cut(s) 372, 494, 650, 747
BstF5I GGATG 1 cut(s) 143
BstKTI GATC 5 cut(s) 103, 264, 329, 351, 423
BstMAI GTCTC 1 cut(s) 497
BstMBI GATC 5 cut(s) 100, 261, 326, 348, 420
BstMWI GCNNNNNNNGC 1 cut(s) 314
BstNSI RCATGY 2 cut(s) 95, 735
BstSCI CCNGG 2 cut(s) 75, 782
BstV1I GCAGC 2 cut(s) 317, 575
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 2 cut(s) 389, 648
BtsCI GGATG 1 cut(s) 143
BtsIMutI CAGTG 2 cut(s) 369, 713
Cac8I GCNNGC 2 cut(s) 173, 639
CciI TCATGA 1 cut(s) 115
Csp6I GTAC 2 cut(s) 95, 529
CviAII CATG 6 cut(s) 92, 116, 314, 379, 538, 732
CviJI RGCY 4 cut(s) 308, 389, 588, 648
CviKI_1 RGCY 4 cut(s) 308, 389, 588, 648
CviQI GTAC 2 cut(s) 95, 529
DdeI CTNAG 4 cut(s) 372, 494, 650, 747
DpnI GATC 5 cut(s) 102, 263, 328, 350, 422
DpnII GATC 5 cut(s) 100, 261, 326, 348, 420
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
Eco147I AGGCCT 1 cut(s) 648
Eco31I GGTCTC 1 cut(s) 497
EcoT22I ATGCAT 2 cut(s) 93, 319
FaeI CATG 6 cut(s) 95, 119, 317, 382, 541, 735
FalI AAGNNNNNCTT 4 cut(s) 594, 626, 621, 653
FaqI GGGAC 1 cut(s) 287
FatI CATG 6 cut(s) 91, 115, 313, 378, 537, 731
FauI CCCGC 1 cut(s) 102
FauNDI CATATG 1 cut(s) 300
FbaI TGATCA 1 cut(s) 348
Fnu4HI GCNGC 2 cut(s) 306, 589
FokI GGATG 1 cut(s) 130
Fsp4HI GCNGC 2 cut(s) 306, 589
FspBI CTAG 1 cut(s) 237
GluI GCNGC 2 cut(s) 306, 589
HaeIII GGCC 2 cut(s) 389, 648
HapII CCGG 2 cut(s) 76, 783
Hin1II CATG 6 cut(s) 95, 119, 317, 382, 541, 735
HincII GTYRAC 2 cut(s) 394, 721
HindII GTYRAC 2 cut(s) 394, 721
HinfI GANTC 2 cut(s) 139, 476
HpaII CCGG 2 cut(s) 76, 783
HphI GGTGA 2 cut(s) 198, 605
Hpy166II GTNNAC 2 cut(s) 394, 721
Hpy188I TCNGA 3 cut(s) 105, 497, 748
Hpy188III TCNNGA 6 cut(s) 49, 116, 128, 482, 618, 737
Hpy8I GTNNAC 2 cut(s) 394, 721
HpyAV CCTTC 4 cut(s) 406, 436, 596, 638
HpyCH4IV ACGT 1 cut(s) 361
HpyCH4V TGCA 3 cut(s) 91, 317, 754
HpyF10VI GCNNNNNNNGC 1 cut(s) 314
HpyF3I CTNAG 4 cut(s) 372, 494, 650, 747
HpySE526I ACGT 1 cut(s) 361
Hsp92II CATG 6 cut(s) 95, 119, 317, 382, 541, 735
Ksp22I TGATCA 1 cut(s) 348
Kzo9I GATC 5 cut(s) 100, 261, 326, 348, 420
LguI GCTCTTC 1 cut(s) 315
LmnI GCTCC 1 cut(s) 227
LpnPI CCDG 6 cut(s) 62, 89, 141, 603, 688, 722
Lsp1109I GCAGC 2 cut(s) 317, 575
LweI GCATC 3 cut(s) 78, 96, 326
MaeI CTAG 1 cut(s) 237
MaeII ACGT 1 cut(s) 361
MaeIII GTNAC 1 cut(s) 512
MalI GATC 5 cut(s) 102, 263, 328, 350, 422
MboI GATC 5 cut(s) 100, 261, 326, 348, 420
MboII GAAGA 6 cut(s) 92, 148, 271, 302, 430, 437
MflI RGATCY 1 cut(s) 100
MhlI GDGCHC 2 cut(s) 378, 627
MluCI AATT 6 cut(s) 10, 64, 211, 344, 517, 778
MmeI TCCRAC 1 cut(s) 240
Mph1103I ATGCAT 2 cut(s) 93, 319
MroXI GAANNNNTTC 1 cut(s) 361
MseI TTAA 1 cut(s) 453
MslI CAYNNNNRTG 1 cut(s) 381
MspI CCGG 2 cut(s) 76, 783
MspR9I CCNGG 2 cut(s) 77, 784
Mva1269I GAATGC 1 cut(s) 439
MwoI GCNNNNNNNGC 1 cut(s) 314
NciI CCSGG 2 cut(s) 77, 784
NdeI CATATG 1 cut(s) 300
NdeII GATC 5 cut(s) 100, 261, 326, 348, 420
NlaIII CATG 6 cut(s) 95, 119, 317, 382, 541, 735
NmuCI GTSAC 1 cut(s) 512
NsiI ATGCAT 2 cut(s) 93, 319
NspI RCATGY 2 cut(s) 95, 735
PagI TCATGA 1 cut(s) 115
PceI AGGCCT 1 cut(s) 648
PciSI GCTCTTC 1 cut(s) 315
PcsI WCGNNNNNNNCGW 1 cut(s) 289
PctI GAATGC 1 cut(s) 439
PdmI GAANNNNTTC 1 cut(s) 361
PfeI GAWTC 2 cut(s) 139, 476
PkrI GCNGC 2 cut(s) 307, 590
PshBI ATTAAT 1 cut(s) 453
Psp1406I AACGTT 1 cut(s) 361
PsuI RGATCY 1 cut(s) 100
RsaI GTAC 2 cut(s) 96, 530
RsaNI GTAC 2 cut(s) 95, 529
RseI CAYNNNNRTG 1 cut(s) 381
SapI GCTCTTC 1 cut(s) 315
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 2 cut(s) 306, 589
Sau3AI GATC 5 cut(s) 100, 261, 326, 348, 420
ScaI AGTACT 1 cut(s) 530
ScrFI CCNGG 2 cut(s) 77, 784
SduI GDGCHC 2 cut(s) 378, 627
SetI ASST 8 cut(s) 56, 123, 211, 310, 364, 573, 590, 717
SfaNI GCATC 3 cut(s) 78, 96, 326
SmiMI CAYNNNNRTG 1 cut(s) 381
SmlI CTYRAG 2 cut(s) 480, 524
SmoI CTYRAG 2 cut(s) 480, 524
Sse9I AATT 6 cut(s) 10, 64, 211, 344, 517, 778
SseBI AGGCCT 1 cut(s) 648
SsiI CCGC 3 cut(s) 109, 591, 641
SspMI CTAG 1 cut(s) 237
StuI AGGCCT 1 cut(s) 648
StyD4I CCNGG 2 cut(s) 75, 782
TaiI ACGT 1 cut(s) 364
TaqI TCGA 2 cut(s) 325, 423
TasI AATT 6 cut(s) 10, 64, 211, 344, 517, 778
TatI WGTACW 2 cut(s) 94, 528
TfiI GAWTC 2 cut(s) 139, 476
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 2 cut(s) 376, 713
TseFI GTSAC 1 cut(s) 512
TseI GCWGC 2 cut(s) 305, 588
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 6 cut(s) 302, 357, 395, 483, 516, 699
TspRI CASTG 2 cut(s) 376, 713
VspI ATTAAT 1 cut(s) 453
XceI RCATGY 2 cut(s) 95, 735
XmnI GAANNNNTTC 1 cut(s) 361
XspI CTAG 1 cut(s) 237
ZrmI AGTACT 1 cut(s) 530
Zsp2I ATGCAT 2 cut(s) 93, 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.