Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0004497

Physical Location & Seq

Genomic Coordinates Forward (+)

5778304 .. 5780314

Gene Structure 2011 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1033400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 897 bp

ATGGCCAAGAAGAAGAACGGCGGCGGCAACAACAATGCCGGCGACAACGCGGCCAGCAACGAAGGTGGTGAGAAGAAGAAGGGCGACGGAGGTGAGAAGAAGGAGGAAACCCCCATCACCGTCATCCTCAAGGTTGACATGCACTGTGAGGGCTGCGCTACTAAGATTGTCAAATGCGTCAAATCTTTTGGAGACGTGGAGACAGTGAAGTCAGAGAGCGAGGCGAACAAGCTGACGGTGGTCGGCAAAGTGGACCCCACCAAGCTCCGGGACAAGGTCGCCGCCAAGACCAAGAAGAAGGTCGACCTCGTCTCCCCCCAGCCCAAAAAGGACACCAACAAAGACGACGGCGGCGACGCCAAGAAAAAGCAGCCGGAGAAATCCAACGACGACAAAAAACCCAAAGAGCCTCCGGTGACGACGGCGGTTCTGAAGCTGAACCTCCACTGCCAGGGATGCATCAAGAAGATCCACAAGATTGTCACCAAGACTAAAGGGTTCAACGACATGAGCGTCGACAAGGAGAAGGAGCTGGTGACAGTGAAGGGCTCGATGGACATGAAGGTGTTGGCGGAGAGCTTGAAGGAGAAGCTGAAGAGGTCGGTCGACATTGTCCCGCCGAAGAAAGAGAAGGAGAAGGGCGACAATGGCGGCGGTGGCGGCGGAGATAAGAAGAAAAAGGAGGGGGAGGAAGGAAATGGTGGTGGGAAGCTTGAGGGAAACAAGATGGATTTTCCAGTGGGTCAGACCGGGTACTGGCAGCAGATTCCATACGAGATGGCATACGGGCCTGGATACGGAGCACCCGGAAACCCGATTCCGCCTCCACCGATGTACATTGGCCATCCGCCAGAGATGTTCAGCGATGAGAACCCTAATGCCTGCTCAATTATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0008150 GO:0008270 GO:0009909 GO:0009910 GO:0009987 GO:0019725 GO:0030001 GO:0030003 GO:0031974 GO:0031981 GO:0042592 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046872 GO:0046914 GO:0046916 GO:0048519 GO:0048580 GO:0048581 GO:0048831 GO:0048878 GO:0050789 GO:0050793 GO:0050801 GO:0051093 GO:0051179 GO:0051234 GO:0051239 GO:0051241 GO:0055065 GO:0055076 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0070013 GO:0098771 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HMA

Protein Analysis

299

Amino Acids

32.03

Weight (kDa)

9.1

Isoelectric Point (pI)

26.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	44 - 95	5.5e-08	Heavy-metal-associated domain
HMA	PF00403	147 - 199	5e-08	Heavy-metal-associated domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004497)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60800 AT5G60800
fragaria_vesca	FvH4_3g31060 FvH4_3g31060
malus_domestica	MD00G1033400.v1.1 MD00G1102700.v1.1
prunus_persica	Prupe.6G108800_v2.0.a1
pyrus_communis	pycom11g11880
rosa_chinensis	RchiOBHm_Chr5g0057721
rosa_laevigata	RLG00000035171
rosa_multiflora	Rmu_co8079862.1_g000001 Rmu_sc0001849.1_g000008 Rmu_sc0002589.1_g000039
rosa_roxburghii	Rroxscaffold_1G00022790
rosa_rugosa	Rorug05G0309100
rosa_samantha	Rh1AG058000 Rh1BG049100 Rh1BG308100 Rh1CG323700 Rh1CG323800 Rh1DG340200 Rh3BG104100 Rh4CG207800 Rh5AG192400 Rh5AG376900 Rh5BG281200 Rh5BG388400 Rh5CG412800 Rh5DG402700 Rh7AG428400
rosa_wichuraiana	Rw2G051380 Rw5G035580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	208, 512
AccI	GTMKAC	3 cut(s)	303, 516, 606
AccII	CGCG	1 cut(s)	50
AclWI	GGATC	1 cut(s)	463
AcoI	YGGCCR	3 cut(s)	3, 51, 841
AcuI	CTGAAG	2 cut(s)	452, 614
AcyI	GRCGYC	1 cut(s)	357
AfaI	GTAC	2 cut(s)	755, 836
AfiI	CCNNNNNNNGG	5 cut(s)	267, 274, 451, 756, 797
AgsI	TTSAA	2 cut(s)	502, 583
AjiI	CACGTC	1 cut(s)	196
AjnI	CCWGG	2 cut(s)	450, 790
AluBI	AGCT	7 cut(s)	232, 265, 436, 532, 579, 592, 712
AluI	AGCT	7 cut(s)	232, 265, 436, 532, 579, 592, 712
Alw21I	GWGCWC	1 cut(s)	805
Alw26I	GTCTC	3 cut(s)	186, 194, 316
AlwI	GGATC	1 cut(s)	463
AoxI	GGCC	4 cut(s)	3, 51, 788, 841
ApeKI	GCWGC	3 cut(s)	153, 370, 760
AspLEI	GCGC	1 cut(s)	158
AspS9I	GGNCC	2 cut(s)	253, 788
AsuC2I	CCSGG	3 cut(s)	269, 751, 807
AsuHPI	GGTGA	6 cut(s)	80, 104, 109, 427, 475, 547
AvaII	GGWCC	1 cut(s)	253
BalI	TGGCCA	2 cut(s)	5, 843
BanII	GRGCYC	1 cut(s)	551
Bbv12I	GWGCWC	1 cut(s)	805
BbvI	GCAGC	3 cut(s)	140, 382, 772
BccI	CCATC	5 cut(s)	122, 547, 721, 772, 852
BceAI	ACGGC	3 cut(s)	34, 364, 438
BciT130I	CCWGG	2 cut(s)	452, 792
BciVI	GTATCC	1 cut(s)	788
BcnI	CCSGG	3 cut(s)	269, 751, 807
BcoDI	GTCTC	3 cut(s)	186, 194, 316
BfuI	GTATCC	1 cut(s)	788
Bme1390I	CCNGG	5 cut(s)	269, 452, 751, 792, 807
Bme18I	GGWCC	1 cut(s)	253
BmgBI	CACGTC	1 cut(s)	196
BmgT120I	GGNCC	2 cut(s)	253, 788
BmiI	GGNNCC	1 cut(s)	255
BmrFI	CCNGG	5 cut(s)	269, 452, 751, 792, 807
BmsI	GCATC	2 cut(s)	446, 468
BoxI	GACNNNNGTC	1 cut(s)	239
BpuEI	CTTGAG	2 cut(s)	113, 734
BpuMI	CCSGG	3 cut(s)	269, 751, 807
BsaHI	GRCGYC	1 cut(s)	357
BsaJI	CCNNGG	1 cut(s)	451
BsaWI	WCCGGW	1 cut(s)	412
BsaXI	ACNNNNNCTCC	2 cut(s)	296, 326
Bsc4I	CCNNNNNNNGG	5 cut(s)	267, 274, 451, 756, 797
Bse118I	RCCGGY	1 cut(s)	38
Bse1I	ACTGG	2 cut(s)	737, 761
BseBI	CCWGG	2 cut(s)	452, 792
BseDI	CCNNGG	1 cut(s)	451
BseGI	GGATG	3 cut(s)	123, 461, 844
BseLI	CCNNNNNNNGG	5 cut(s)	267, 274, 451, 756, 797
BseNI	ACTGG	2 cut(s)	737, 761
BseXI	GCAGC	3 cut(s)	140, 382, 772
BseYI	CCCAGC	1 cut(s)	318
Bsh1236I	CGCG	1 cut(s)	50
Bsh1285I	CGRYCG	1 cut(s)	606
BshFI	GGCC	4 cut(s)	5, 53, 790, 843
BsiEI	CGRYCG	1 cut(s)	606
BsiHKAI	GWGCWC	1 cut(s)	805
BsiSI	CCGG	6 cut(s)	39, 268, 374, 413, 750, 807
BslFI	GGGAC	2 cut(s)	284, 599
BslI	CCNNNNNNNGG	5 cut(s)	267, 274, 451, 756, 797
BsmAI	GTCTC	3 cut(s)	186, 194, 316
BsmBI	CGTCTC	2 cut(s)	186, 316
BsmFI	GGGAC	2 cut(s)	284, 599
BsnI	GGCC	4 cut(s)	5, 53, 790, 843
Bsp1286I	GDGCHC	2 cut(s)	551, 805
Bsp1407I	TGTACA	1 cut(s)	834
Bsp143I	GATC	1 cut(s)	468
BspANI	GGCC	4 cut(s)	5, 53, 790, 843
BspFNI	CGCG	1 cut(s)	50
BspLI	GGNNCC	1 cut(s)	255
BspPI	GGATC	1 cut(s)	463
BsrFI	RCCGGY	1 cut(s)	38
BsrGI	TGTACA	1 cut(s)	834
BsrI	ACTGG	2 cut(s)	737, 761
BssAI	RCCGGY	1 cut(s)	38
BssECI	CCNNGG	1 cut(s)	451
BssMI	GATC	1 cut(s)	468
BssNI	GRCGYC	1 cut(s)	357
Bst2UI	CCWGG	2 cut(s)	452, 792
Bst4CI	ACNGT	5 cut(s)	121, 146, 205, 238, 541
Bst6I	CTCTTC	1 cut(s)	590
BstACI	GRCGYC	1 cut(s)	357
BstAUI	TGTACA	1 cut(s)	834
BstC8I	GCNNGC	3 cut(s)	40, 55, 883
BstDEI	CTNAG	1 cut(s)	162
BstF5I	GGATG	3 cut(s)	123, 461, 844
BstFNI	CGCG	1 cut(s)	50
BstHHI	GCGC	1 cut(s)	158
BstKTI	GATC	1 cut(s)	471
BstMAI	GTCTC	3 cut(s)	186, 194, 316
BstMBI	GATC	1 cut(s)	468
BstMCI	CGRYCG	1 cut(s)	606
BstMWI	GCNNNNNNNGC	4 cut(s)	456, 648, 657, 660
BstNI	CCWGG	2 cut(s)	452, 792
BstNSI	RCATGY	1 cut(s)	142
BstPAI	GACNNNNGTC	1 cut(s)	239
BstSCI	CCNGG	5 cut(s)	267, 450, 749, 790, 805
BstUI	CGCG	1 cut(s)	50
BstV1I	GCAGC	3 cut(s)	140, 382, 772
BstX2I	RGATCY	1 cut(s)	468
BstYI	RGATCY	1 cut(s)	468
BsuI	GTATCC	1 cut(s)	788
BsuRI	GGCC	4 cut(s)	5, 53, 790, 843
BtgZI	GCGATG	1 cut(s)	879
BtrI	CACGTC	1 cut(s)	196
BtsCI	GGATG	3 cut(s)	123, 461, 844
BtsI	GCAGTG	1 cut(s)	445
BtsIMutI	CAGTG	5 cut(s)	142, 210, 445, 546, 744
Cac8I	GCNNGC	3 cut(s)	40, 55, 883
CfoI	GCGC	1 cut(s)	158
Cfr10I	RCCGGY	1 cut(s)	38
Cfr13I	GGNCC	2 cut(s)	253, 788
CseI	GACGC	3 cut(s)	166, 365, 502
Csp6I	GTAC	2 cut(s)	754, 835
CspCI	CAANNNNNGTGG	2 cut(s)	46, 81
CviAII	CATG	3 cut(s)	139, 508, 559
CviQI	GTAC	2 cut(s)	754, 835
DdeI	CTNAG	1 cut(s)	162
DpnI	GATC	1 cut(s)	470
DpnII	GATC	1 cut(s)	468
DrdI	GACNNNNNNGTC	2 cut(s)	208, 512
DseDI	GACNNNNNNGTC	2 cut(s)	208, 512
EaeI	YGGCCR	3 cut(s)	3, 51, 841
Eam1104I	CTCTTC	1 cut(s)	590
EarI	CTCTTC	1 cut(s)	590
EciI	GGCGGA	4 cut(s)	587, 678, 810, 837
Eco24I	GRGCYC	1 cut(s)	551
Eco47I	GGWCC	1 cut(s)	253
Eco57I	CTGAAG	2 cut(s)	452, 614
EcoRII	CCWGG	2 cut(s)	450, 790
EcoT22I	ATGCAT	1 cut(s)	461
EcoT38I	GRGCYC	1 cut(s)	551
Esp3I	CGTCTC	2 cut(s)	186, 316
FaeI	CATG	3 cut(s)	142, 511, 562
FaiI	YATR	6 cut(s)	140, 509, 560, 772, 784, 893
FaqI	GGGAC	2 cut(s)	284, 599
FatI	CATG	3 cut(s)	138, 507, 558
FauI	CCCGC	1 cut(s)	624
FblI	GTMKAC	3 cut(s)	303, 516, 606
FokI	GGATG	3 cut(s)	110, 468, 831
FriOI	GRGCYC	1 cut(s)	551
GlaI	GCGC	1 cut(s)	157
GsaI	CCCAGC	1 cut(s)	322
HaeIII	GGCC	4 cut(s)	5, 53, 790, 843
HapII	CCGG	6 cut(s)	39, 268, 374, 413, 750, 807
HgaI	GACGC	3 cut(s)	166, 365, 502
HhaI	GCGC	1 cut(s)	158
Hin1I	GRCGYC	1 cut(s)	357
Hin1II	CATG	3 cut(s)	142, 511, 562
Hin6I	GCGC	1 cut(s)	156
HinP1I	GCGC	1 cut(s)	156
HincII	GTYRAC	4 cut(s)	136, 304, 517, 607
HindII	GTYRAC	4 cut(s)	136, 304, 517, 607
HindIII	AAGCTT	1 cut(s)	710
HinfI	GANTC	2 cut(s)	766, 817
HpaII	CCGG	6 cut(s)	39, 268, 374, 413, 750, 807
HphI	GGTGA	6 cut(s)	80, 104, 109, 427, 475, 547
Hpy166II	GTNNAC	5 cut(s)	136, 253, 304, 517, 607
Hpy188I	TCNGA	3 cut(s)	214, 432, 747
Hpy188III	TCNNGA	1 cut(s)	463
Hpy8I	GTNNAC	5 cut(s)	136, 253, 304, 517, 607
Hpy99I	CGWCG	6 cut(s)	89, 350, 359, 392, 424, 518
HpyCH4III	ACNGT	5 cut(s)	121, 146, 205, 238, 541
HpyCH4IV	ACGT	1 cut(s)	195
HpyCH4V	TGCA	2 cut(s)	142, 459
HpyF10VI	GCNNNNNNNGC	4 cut(s)	456, 648, 657, 660
HpyF3I	CTNAG	1 cut(s)	162
HpySE526I	ACGT	1 cut(s)	195
Hsp92I	GRCGYC	1 cut(s)	357
Hsp92II	CATG	3 cut(s)	142, 511, 562
HspAI	GCGC	1 cut(s)	156
KroI	GCCGGC	1 cut(s)	38
KroNI	GCCGGC	1 cut(s)	40
Kzo9I	GATC	1 cut(s)	468
LmnI	GCTCC	3 cut(s)	270, 529, 800
Lsp1109I	GCAGC	3 cut(s)	140, 382, 772
LweI	GCATC	2 cut(s)	446, 468
MaeII	ACGT	1 cut(s)	195
MaeIII	GTNAC	3 cut(s)	415, 481, 535
MalI	GATC	1 cut(s)	470
MboI	GATC	1 cut(s)	468
MflI	RGATCY	1 cut(s)	468
MhlI	GDGCHC	2 cut(s)	551, 805
MlsI	TGGCCA	2 cut(s)	5, 843
MluCI	AATT	1 cut(s)	888
MluNI	TGGCCA	2 cut(s)	5, 843
MmeI	TCCRAC	1 cut(s)	408
Mox20I	TGGCCA	2 cut(s)	5, 843
Mph1103I	ATGCAT	1 cut(s)	461
MroNI	GCCGGC	1 cut(s)	38
MscI	TGGCCA	2 cut(s)	5, 843
MslI	CAYNNNNRTG	1 cut(s)	563
Msp20I	TGGCCA	2 cut(s)	5, 843
MspI	CCGG	6 cut(s)	39, 268, 374, 413, 750, 807
MspR9I	CCNGG	5 cut(s)	269, 452, 751, 792, 807
MvaI	CCWGG	2 cut(s)	452, 792
MvnI	CGCG	1 cut(s)	50
MwoI	GCNNNNNNNGC	4 cut(s)	456, 648, 657, 660
NaeI	GCCGGC	1 cut(s)	40
NciI	CCSGG	3 cut(s)	269, 751, 807
NdeII	GATC	1 cut(s)	468
NgoMIV	GCCGGC	1 cut(s)	38
NlaIII	CATG	3 cut(s)	142, 511, 562
NlaIV	GGNNCC	1 cut(s)	255
NmuCI	GTSAC	3 cut(s)	415, 481, 535
NsiI	ATGCAT	1 cut(s)	461
NspI	RCATGY	1 cut(s)	142
PcsI	WCGNNNNNNNCGW	2 cut(s)	351, 510
PdiI	GCCGGC	1 cut(s)	40
PfeI	GAWTC	2 cut(s)	766, 817
PflFI	GACNNNGTC	3 cut(s)	275, 308, 611
PfoI	TCCNGGA	1 cut(s)	267
PshAI	GACNNNNGTC	1 cut(s)	239
Psp6I	CCWGG	2 cut(s)	450, 790
PspFI	CCCAGC	1 cut(s)	318
PspGI	CCWGG	2 cut(s)	450, 790
PspN4I	GGNNCC	1 cut(s)	255
PspPI	GGNCC	2 cut(s)	253, 788
PsuI	RGATCY	1 cut(s)	468
PsyI	GACNNNGTC	3 cut(s)	275, 308, 611
RsaI	GTAC	2 cut(s)	755, 836
RsaNI	GTAC	2 cut(s)	754, 835
RseI	CAYNNNNRTG	1 cut(s)	563
SalI	GTCGAC	3 cut(s)	302, 515, 605
Sau3AI	GATC	1 cut(s)	468
Sau96I	GGNCC	2 cut(s)	253, 788
ScrFI	CCNGG	5 cut(s)	269, 452, 751, 792, 807
SduI	GDGCHC	2 cut(s)	551, 805
SfaNI	GCATC	2 cut(s)	446, 468
SinI	GGWCC	1 cut(s)	253
SmiMI	CAYNNNNRTG	1 cut(s)	563
SmlI	CTYRAG	2 cut(s)	128, 713
SmoI	CTYRAG	2 cut(s)	128, 713
Sse9I	AATT	1 cut(s)	888
StyD4I	CCNGG	5 cut(s)	267, 450, 749, 790, 805
TaaI	ACNGT	5 cut(s)	121, 146, 205, 238, 541
TaiI	ACGT	1 cut(s)	198
TaqI	TCGA	4 cut(s)	303, 516, 551, 606
TaqII	GACCGA	1 cut(s)	592
TasI	AATT	1 cut(s)	888
TatI	WGTACW	1 cut(s)	834
TauI	GCSGC	7 cut(s)	24, 27, 53, 284, 354, 654, 663
TfiI	GAWTC	2 cut(s)	766, 817
TscAI	CASTG	5 cut(s)	149, 210, 452, 546, 744
TseFI	GTSAC	3 cut(s)	415, 481, 535
TseI	GCWGC	3 cut(s)	153, 370, 760
Tsp45I	GTSAC	3 cut(s)	415, 481, 535
TspDTI	ATGAA	1 cut(s)	575
TspGWI	ACGGA	2 cut(s)	102, 813
TspRI	CASTG	5 cut(s)	149, 210, 452, 546, 744
Tth111I	GACNNNGTC	3 cut(s)	275, 308, 611
VpaK11BI	GGWCC	1 cut(s)	253
XceI	RCATGY	1 cut(s)	142
XmiI	GTMKAC	3 cut(s)	303, 516, 606
Zsp2I	ATGCAT	1 cut(s)	461

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.