Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0004497

Physical Location & Seq

Genomic Coordinates Forward (+)

21635061 .. 21637161

Gene Structure 2101 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1102700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 906 bp

ATGGCCAAGAAGAAGAAGGGCGGCGGCGGCGGCGACAATAACGCCGGCAACACCGCCAGCAACGACGGGGGTGAGAAGAAGAAGGGCGACGGAGGTGAGAAGAAGGAGGAAACCCCCATCACTGTAATCCTGAAGATTGACATGCACTGTGAGGGCTGCGCTACCAAGATTGTCAAACGCGTCAAAGGTTTTGCAGGGGTGGAGACAGTGAAGTCAGAGAGCCAGGCGAACAAGCTGACGGTGGTCGGGAAAGTGGACCCCACCACGCTCCGGGACAAGCTCGCCGCCAAGACCAATAAGAAGGTCGACCTCGTCTCCCCCCAGCCCAAAAAGGACAACAAAGACGACGGCGGCGACGCCAAGAAAAAGCCGCCGGAGAAATCCAACGACGACAAAAAAACCAAAGAGCCTCCGGTGACGACCGCGGTTCTGAAGCTGAACCTCCACTGCCAGGGATGCATCCAGAAGATCCACAAGACCGTCACCAAGACGAAAGGGTTCACCGAGATGAGCATCGACAAGGAGAAGGAGCTGCTGACGGTGAAGGGCTCGATGGACATGAAGGTTCTGGCCGAGAGCTTGAAGGAGAAGCTGAAGAGGTCCGTCGACATTGTCCCGCCGAAGAAAGAGAAGGAGAAGGGCGAAAATAACGGCGGAGGCGGAGGTGGCGGCGGGGATAAGAAGAAAAAGGAAGGGGAGGAGGGAAAGGAGGGTGGGAAACTTGAGGGGAACAGGATGGATTTTCCTCCGGGTCCGACCGGGTACGGGCAGCAGATTCCATACGTGATGCCATACGGGCCTGGATACGGAGCACCCGGATACCCGATTCCGCCCCCACCGATGTACATTGGCAACCCGCCGGAGATGTTCAGCGATGAGAACCCTAATGCCTGCTCAATTATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0008150 GO:0008270 GO:0009909 GO:0009910 GO:0009987 GO:0019725 GO:0030001 GO:0030003 GO:0031974 GO:0031981 GO:0042592 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046872 GO:0046914 GO:0046916 GO:0048519 GO:0048580 GO:0048581 GO:0048831 GO:0048878 GO:0050789 GO:0050793 GO:0050801 GO:0051093 GO:0051179 GO:0051234 GO:0051239 GO:0051241 GO:0055065 GO:0055076 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0070013 GO:0098771 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HMA

Protein Analysis

302

Amino Acids

32.13

Weight (kDa)

9.25

Isoelectric Point (pI)

32.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	45 - 97	2.2e-10	Heavy-metal-associated domain
HMA	PF00403	147 - 199	7.1e-08	Heavy-metal-associated domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004497)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60800 AT5G60800
fragaria_vesca	FvH4_3g31060 FvH4_3g31060
malus_domestica	MD00G1033400.v1.1 MD00G1102700.v1.1
prunus_persica	Prupe.6G108800_v2.0.a1
pyrus_communis	pycom11g11880
rosa_chinensis	RchiOBHm_Chr5g0057721
rosa_laevigata	RLG00000035171
rosa_multiflora	Rmu_co8079862.1_g000001 Rmu_sc0001849.1_g000008 Rmu_sc0002589.1_g000039
rosa_roxburghii	Rroxscaffold_1G00022790
rosa_rugosa	Rorug05G0309100
rosa_samantha	Rh1AG058000 Rh1BG049100 Rh1BG308100 Rh1CG323700 Rh1CG323800 Rh1DG340200 Rh3BG104100 Rh4CG207800 Rh5AG192400 Rh5AG376900 Rh5BG281200 Rh5BG388400 Rh5CG412800 Rh5DG402700 Rh7AG428400
rosa_wichuraiana	Rw2G051380 Rw5G035580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	211
AccI	GTMKAC	2 cut(s)	306, 606
AccII	CGCG	2 cut(s)	180, 425
AclWI	GGATC	1 cut(s)	463
AcoI	YGGCCR	2 cut(s)	3, 570
AcuI	CTGAAG	3 cut(s)	152, 452, 614
AcyI	GRCGYC	1 cut(s)	357
AfaI	GTAC	2 cut(s)	764, 845
AfiI	CCNNNNNNNGG	4 cut(s)	270, 451, 765, 806
AflIII	ACRYGT	1 cut(s)	178
AgsI	TTSAA	1 cut(s)	583
AjnI	CCWGG	3 cut(s)	222, 450, 799
AluBI	AGCT	6 cut(s)	235, 280, 436, 532, 579, 592
AluI	AGCT	6 cut(s)	235, 280, 436, 532, 579, 592
Alw21I	GWGCWC	1 cut(s)	814
Alw26I	GTCTC	2 cut(s)	197, 319
AlwI	GGATC	1 cut(s)	463
AoxI	GGCC	3 cut(s)	3, 570, 797
ApeKI	GCWGC	3 cut(s)	156, 532, 769
Asp700I	GAANNNNTTC	1 cut(s)	497
AspLEI	GCGC	1 cut(s)	161
AspS9I	GGNCC	4 cut(s)	256, 600, 752, 797
AsuC2I	CCSGG	4 cut(s)	272, 750, 760, 816
AsuHPI	GGTGA	6 cut(s)	83, 107, 427, 475, 493, 553
AvaII	GGWCC	3 cut(s)	256, 600, 752
BalI	TGGCCA	1 cut(s)	5
BanII	GRGCYC	1 cut(s)	551
Bbv12I	GWGCWC	1 cut(s)	814
BbvI	GCAGC	3 cut(s)	143, 519, 781
BccI	CCATC	3 cut(s)	125, 547, 730
BceAI	ACGGC	2 cut(s)	364, 667
BciT130I	CCWGG	3 cut(s)	224, 452, 801
BciVI	GTATCC	2 cut(s)	797, 812
BcnI	CCSGG	4 cut(s)	272, 750, 760, 816
BcoDI	GTCTC	2 cut(s)	197, 319
BfuI	GTATCC	2 cut(s)	797, 812
BglI	GCCNNNNNGGC	1 cut(s)	796
Bme1390I	CCNGG	7 cut(s)	224, 272, 452, 750, 760, 801, 816
Bme18I	GGWCC	3 cut(s)	256, 600, 752
BmgT120I	GGNCC	4 cut(s)	256, 600, 752, 797
BmiI	GGNNCC	2 cut(s)	258, 753
BmrFI	CCNGG	7 cut(s)	224, 272, 452, 750, 760, 801, 816
BmsI	GCATC	4 cut(s)	446, 468, 522, 777
BoxI	GACNNNNGTC	1 cut(s)	242
BpuEI	CTTGAG	1 cut(s)	743
BpuMI	CCSGG	4 cut(s)	272, 750, 760, 816
BsaAI	YACGTR	1 cut(s)	784
BsaBI	GATNNNNATC	1 cut(s)	512
BsaHI	GRCGYC	1 cut(s)	357
BsaJI	CCNNGG	2 cut(s)	423, 451
BsaWI	WCCGGW	1 cut(s)	412
BsaXI	ACNNNNNCTCC	2 cut(s)	299, 329
Bsc4I	CCNNNNNNNGG	4 cut(s)	270, 451, 765, 806
Bse118I	RCCGGY	1 cut(s)	44
Bse8I	GATNNNNATC	1 cut(s)	512
BseBI	CCWGG	3 cut(s)	224, 452, 801
BseDI	CCNNGG	2 cut(s)	423, 451
BseGI	GGATG	3 cut(s)	459, 461, 741
BseJI	GATNNNNATC	1 cut(s)	512
BseLI	CCNNNNNNNGG	4 cut(s)	270, 451, 765, 806
BseRI	GAGGAG	1 cut(s)	713
BseXI	GCAGC	3 cut(s)	143, 519, 781
BseYI	CCCAGC	1 cut(s)	321
Bsh1236I	CGCG	2 cut(s)	180, 425
Bsh1285I	CGRYCG	2 cut(s)	423, 759
BshFI	GGCC	3 cut(s)	5, 572, 799
BsiEI	CGRYCG	2 cut(s)	423, 759
BsiHKAI	GWGCWC	1 cut(s)	814
BsiSI	CCGG	8 cut(s)	45, 271, 374, 413, 749, 759, 816, 860
BslFI	GGGAC	2 cut(s)	287, 599
BslI	CCNNNNNNNGG	4 cut(s)	270, 451, 765, 806
BsmAI	GTCTC	2 cut(s)	197, 319
BsmBI	CGTCTC	1 cut(s)	319
BsmFI	GGGAC	2 cut(s)	287, 599
BsnI	GGCC	3 cut(s)	5, 572, 799
Bsp1286I	GDGCHC	2 cut(s)	551, 814
Bsp1407I	TGTACA	1 cut(s)	843
Bsp143I	GATC	1 cut(s)	468
BspANI	GGCC	3 cut(s)	5, 572, 799
BspFNI	CGCG	2 cut(s)	180, 425
BspLI	GGNNCC	2 cut(s)	258, 753
BspPI	GGATC	1 cut(s)	463
BsrFI	RCCGGY	1 cut(s)	44
BsrGI	TGTACA	1 cut(s)	843
BssAI	RCCGGY	1 cut(s)	44
BssECI	CCNNGG	2 cut(s)	423, 451
BssMI	GATC	1 cut(s)	468
BssNI	GRCGYC	1 cut(s)	357
Bst2UI	CCWGG	3 cut(s)	224, 452, 801
Bst4CI	ACNGT	6 cut(s)	124, 149, 208, 241, 481, 541
Bst6I	CTCTTC	1 cut(s)	590
BstACI	GRCGYC	1 cut(s)	357
BstAUI	TGTACA	1 cut(s)	843
BstBAI	YACGTR	1 cut(s)	784
BstC8I	GCNNGC	4 cut(s)	46, 58, 282, 892
BstDSI	CCRYGG	1 cut(s)	423
BstF5I	GGATG	3 cut(s)	459, 461, 741
BstFNI	CGCG	2 cut(s)	180, 425
BstHHI	GCGC	1 cut(s)	161
BstKTI	GATC	1 cut(s)	471
BstMAI	GTCTC	2 cut(s)	197, 319
BstMBI	GATC	1 cut(s)	468
BstMCI	CGRYCG	2 cut(s)	423, 759
BstMWI	GCNNNNNNNGC	5 cut(s)	27, 30, 456, 666, 796
BstNI	CCWGG	3 cut(s)	224, 452, 801
BstNSI	RCATGY	1 cut(s)	145
BstPAI	GACNNNNGTC	1 cut(s)	242
BstSCI	CCNGG	7 cut(s)	222, 270, 450, 748, 758, 799, 814
BstUI	CGCG	2 cut(s)	180, 425
BstV1I	GCAGC	3 cut(s)	143, 519, 781
BstX2I	RGATCY	1 cut(s)	468
BstYI	RGATCY	1 cut(s)	468
BsuI	GTATCC	2 cut(s)	797, 812
BsuRI	GGCC	3 cut(s)	5, 572, 799
BtgI	CCRYGG	1 cut(s)	423
BtgZI	GCGATG	1 cut(s)	888
BtsCI	GGATG	3 cut(s)	459, 461, 741
BtsI	GCAGTG	1 cut(s)	445
BtsIMutI	CAGTG	4 cut(s)	120, 145, 213, 445
Cac8I	GCNNGC	4 cut(s)	46, 58, 282, 892
CfoI	GCGC	1 cut(s)	161
Cfr10I	RCCGGY	1 cut(s)	44
Cfr13I	GGNCC	4 cut(s)	256, 600, 752, 797
Cfr42I	CCGCGG	1 cut(s)	426
CseI	GACGC	2 cut(s)	169, 365
Csp6I	GTAC	2 cut(s)	763, 844
CviAII	CATG	2 cut(s)	142, 559
CviQI	GTAC	2 cut(s)	763, 844
DpnI	GATC	1 cut(s)	470
DpnII	GATC	1 cut(s)	468
DrdI	GACNNNNNNGTC	1 cut(s)	211
DseDI	GACNNNNNNGTC	1 cut(s)	211
EaeI	YGGCCR	2 cut(s)	3, 570
Eam1104I	CTCTTC	1 cut(s)	590
EarI	CTCTTC	1 cut(s)	590
EciI	GGCGGA	3 cut(s)	669, 675, 819
Eco24I	GRGCYC	1 cut(s)	551
Eco47I	GGWCC	3 cut(s)	256, 600, 752
Eco57I	CTGAAG	3 cut(s)	152, 452, 614
EcoRII	CCWGG	3 cut(s)	222, 450, 799
EcoT22I	ATGCAT	1 cut(s)	461
EcoT38I	GRGCYC	1 cut(s)	551
Esp3I	CGTCTC	1 cut(s)	319
FaeI	CATG	2 cut(s)	145, 562
FaiI	YATR	5 cut(s)	143, 560, 781, 793, 902
FaqI	GGGAC	2 cut(s)	287, 599
FatI	CATG	2 cut(s)	141, 558
FauI	CCCGC	3 cut(s)	624, 665, 864
FblI	GTMKAC	2 cut(s)	306, 606
FokI	GGATG	3 cut(s)	446, 468, 748
FriOI	GRGCYC	1 cut(s)	551
GlaI	GCGC	1 cut(s)	160
GsaI	CCCAGC	1 cut(s)	325
HaeIII	GGCC	3 cut(s)	5, 572, 799
HapII	CCGG	8 cut(s)	45, 271, 374, 413, 749, 759, 816, 860
HgaI	GACGC	2 cut(s)	169, 365
HhaI	GCGC	1 cut(s)	161
Hin1I	GRCGYC	1 cut(s)	357
Hin1II	CATG	2 cut(s)	145, 562
Hin6I	GCGC	1 cut(s)	159
HinP1I	GCGC	1 cut(s)	159
HincII	GTYRAC	2 cut(s)	307, 607
HindII	GTYRAC	2 cut(s)	307, 607
HinfI	GANTC	2 cut(s)	775, 826
HpaII	CCGG	8 cut(s)	45, 271, 374, 413, 749, 759, 816, 860
HphI	GGTGA	6 cut(s)	83, 107, 427, 475, 493, 553
Hpy166II	GTNNAC	4 cut(s)	256, 307, 501, 607
Hpy188I	TCNGA	3 cut(s)	217, 432, 756
Hpy188III	TCNNGA	3 cut(s)	130, 247, 463
Hpy8I	GTNNAC	4 cut(s)	256, 307, 501, 607
Hpy99I	CGWCG	6 cut(s)	68, 92, 350, 359, 392, 608
HpyCH4III	ACNGT	6 cut(s)	124, 149, 208, 241, 481, 541
HpyCH4IV	ACGT	1 cut(s)	783
HpyCH4V	TGCA	3 cut(s)	145, 194, 459
HpyF10VI	GCNNNNNNNGC	5 cut(s)	27, 30, 456, 666, 796
HpySE526I	ACGT	1 cut(s)	783
Hsp92I	GRCGYC	1 cut(s)	357
Hsp92II	CATG	2 cut(s)	145, 562
HspAI	GCGC	1 cut(s)	159
KroI	GCCGGC	1 cut(s)	44
KroNI	GCCGGC	1 cut(s)	46
KspI	CCGCGG	1 cut(s)	426
Kzo9I	GATC	1 cut(s)	468
LmnI	GCTCC	3 cut(s)	273, 529, 809
Lsp1109I	GCAGC	3 cut(s)	143, 519, 781
LweI	GCATC	4 cut(s)	446, 468, 522, 777
MaeII	ACGT	1 cut(s)	783
MaeIII	GTNAC	2 cut(s)	415, 481
MalI	GATC	1 cut(s)	470
MboI	GATC	1 cut(s)	468
MflI	RGATCY	1 cut(s)	468
MhlI	GDGCHC	2 cut(s)	551, 814
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	1 cut(s)	897
MluI	ACGCGT	1 cut(s)	178
MluNI	TGGCCA	1 cut(s)	5
MmeI	TCCRAC	2 cut(s)	408, 779
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	461
MroNI	GCCGGC	1 cut(s)	44
MroXI	GAANNNNTTC	1 cut(s)	497
MscI	TGGCCA	1 cut(s)	5
MslI	CAYNNNNRTG	1 cut(s)	506
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	425
MspI	CCGG	8 cut(s)	45, 271, 374, 413, 749, 759, 816, 860
MspR9I	CCNGG	7 cut(s)	224, 272, 452, 750, 760, 801, 816
MvaI	CCWGG	3 cut(s)	224, 452, 801
MvnI	CGCG	2 cut(s)	180, 425
MwoI	GCNNNNNNNGC	5 cut(s)	27, 30, 456, 666, 796
NaeI	GCCGGC	1 cut(s)	46
NciI	CCSGG	4 cut(s)	272, 750, 760, 816
NdeII	GATC	1 cut(s)	468
NgoMIV	GCCGGC	1 cut(s)	44
NlaIII	CATG	2 cut(s)	145, 562
NlaIV	GGNNCC	2 cut(s)	258, 753
NmeAIII	GCCGAG	1 cut(s)	598
NmuCI	GTSAC	2 cut(s)	415, 481
NsiI	ATGCAT	1 cut(s)	461
NspI	RCATGY	1 cut(s)	145
PcsI	WCGNNNNNNNCGW	1 cut(s)	351
PdiI	GCCGGC	1 cut(s)	46
PdmI	GAANNNNTTC	1 cut(s)	497
PfeI	GAWTC	2 cut(s)	775, 826
PflFI	GACNNNGTC	2 cut(s)	311, 611
PfoI	TCCNGGA	1 cut(s)	270
Ppu21I	YACGTR	1 cut(s)	784
PshAI	GACNNNNGTC	1 cut(s)	242
Psp6I	CCWGG	3 cut(s)	222, 450, 799
PspFI	CCCAGC	1 cut(s)	321
PspGI	CCWGG	3 cut(s)	222, 450, 799
PspN4I	GGNNCC	2 cut(s)	258, 753
PspPI	GGNCC	4 cut(s)	256, 600, 752, 797
PsuI	RGATCY	1 cut(s)	468
PsyI	GACNNNGTC	2 cut(s)	311, 611
RsaI	GTAC	2 cut(s)	764, 845
RsaNI	GTAC	2 cut(s)	763, 844
RseI	CAYNNNNRTG	1 cut(s)	506
SacII	CCGCGG	1 cut(s)	426
SalI	GTCGAC	2 cut(s)	305, 605
Sau3AI	GATC	1 cut(s)	468
Sau96I	GGNCC	4 cut(s)	256, 600, 752, 797
ScrFI	CCNGG	7 cut(s)	224, 272, 452, 750, 760, 801, 816
SduI	GDGCHC	2 cut(s)	551, 814
SfaNI	GCATC	4 cut(s)	446, 468, 522, 777
Sfr303I	CCGCGG	1 cut(s)	426
SgrBI	CCGCGG	1 cut(s)	426
SinI	GGWCC	3 cut(s)	256, 600, 752
SmiMI	CAYNNNNRTG	1 cut(s)	506
SmlI	CTYRAG	1 cut(s)	722
SmoI	CTYRAG	1 cut(s)	722
Sse9I	AATT	1 cut(s)	897
StyD4I	CCNGG	7 cut(s)	222, 270, 450, 748, 758, 799, 814
TaaI	ACNGT	6 cut(s)	124, 149, 208, 241, 481, 541
TaiI	ACGT	1 cut(s)	786
TaqI	TCGA	4 cut(s)	306, 516, 551, 606
TasI	AATT	1 cut(s)	897
TatI	WGTACW	1 cut(s)	843
TauI	GCSGC	8 cut(s)	24, 27, 30, 33, 287, 354, 373, 672
TfiI	GAWTC	2 cut(s)	775, 826
TscAI	CASTG	4 cut(s)	127, 152, 213, 452
TseFI	GTSAC	2 cut(s)	415, 481
TseI	GCWGC	3 cut(s)	156, 532, 769
Tsp45I	GTSAC	2 cut(s)	415, 481
TspDTI	ATGAA	1 cut(s)	575
TspGWI	ACGGA	3 cut(s)	105, 592, 822
TspRI	CASTG	4 cut(s)	127, 152, 213, 452
Tth111I	GACNNNGTC	2 cut(s)	311, 611
VpaK11BI	GGWCC	3 cut(s)	256, 600, 752
XceI	RCATGY	1 cut(s)	145
XmiI	GTMKAC	2 cut(s)	306, 606
XmnI	GAANNNNTTC	1 cut(s)	497
Zsp2I	ATGCAT	1 cut(s)	461

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.