MD00G1104700.v1.1
MYB Family

Myb-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0005146
Physical Location & Seq
Reverse (-)
21970709 .. 21972003
1295 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1104700.v1.1.491

Sequence Viewer

Length: 756 bp
ATGGGCAGGCAACCTTGTTGCGACAAGCTTGGAGTGAAGAAAGGGCCATGGACTGCCGAGGAGGACAAGAAATTGGTGAATTTTCTCCTCACACATGGCCAATGTTGTTGGCGGGCCGTCCCCAAGCTTGCCGGCCTCCGCCGCTGCGGCAAGAGCTGCCGCCTCCGGTGGATTAATTACCTCCGCCCCGACTTGAAAAGAGGCCTCCTTAATGATGCTGAAGAACAACTTGTTATTGACCTGCACGCCCGCCTTGGCAATAGGTGGTCGAAAATTGCAGCAAGATTGCCGGGAAGAACTGACAATGAGATCAAGAATCACTGGAACACGCACATCAAGAAGAAGCTTGTCAAGATGGGGATTGATCCAATTACTCATGAGCCTCTCCACAAACAAGTCACCACCCTACAAGAAATGCCTTGTGAAGCAAGTAATCAACCTGCAAACTCAGACATGAGTATTCAGCAGATGAATACAAACATCCCAGAACATGGAATCAGCACCAACAGTGACGGTAACTCTACAAGTGAAAATTCCCCAAGCAATGACTCAGAGCCAGCTGAGCCAAACCCTAATTACAGTGAGGAAGAAGACCCGTTGGTGAGTTTCATATTATCCGATACATTTCTTGAGGATTTAACATGGGATTTTTCGACCTCGTCAGAATACAGCTCAGCTGATAATCCAACGGAGGAGAATAGCTTAGCATGGTTCATGGACTGTAATGATTTTGGAGTTGAAGATTTCGAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

252

Amino Acids

28.45

Weight (kDa)

5.06

Isoelectric Point (pI)

51.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 2.9e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 2.5e-09 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 72 - 116 2.4e-07 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 73 - 111 1.6e-15 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 249, 448
AccB7I CCANNNNNTGG 1 cut(s) 491
AciI CCGC 7 cut(s) 112, 139, 142, 147, 160, 184, 250
AclWI GGATC 1 cut(s) 359
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 2 cut(s) 79, 532
AcuI CTGAAG 1 cut(s) 240
AfiI CCNNNNNNNGG 1 cut(s) 491
AgsI TTSAA 2 cut(s) 196, 740
AluBI AGCT 9 cut(s) 28, 127, 156, 346, 560, 672, 677, 702, 751
AluI AGCT 9 cut(s) 28, 127, 156, 346, 560, 672, 677, 702, 751
AlwI GGATC 1 cut(s) 359
AoxI GGCC 5 cut(s) 44, 97, 114, 133, 202
ApeKI GCWGC 3 cut(s) 144, 156, 278
ApoI RAATTY 2 cut(s) 79, 532
AseI ATTAAT 1 cut(s) 174
AspS9I GGNCC 2 cut(s) 44, 114
AsuC2I CCSGG 1 cut(s) 291
AsuHPI GGTGA 3 cut(s) 88, 391, 613
BalI TGGCCA 1 cut(s) 99
BbsI GAAGAC 1 cut(s) 597
BbvI GCAGC 3 cut(s) 131, 143, 290
BccI CCATC 1 cut(s) 349
BceAI ACGGC 1 cut(s) 101
BcnI CCSGG 1 cut(s) 291
BfuAI ACCTGC 2 cut(s) 249, 448
BglI GCCNNNNNGGC 1 cut(s) 147
BisI GCNGC 6 cut(s) 142, 145, 148, 157, 160, 279
BlpI GCTNAGC 3 cut(s) 561, 673, 703
BlsI GCNGC 6 cut(s) 143, 146, 149, 158, 161, 280
Bme1390I CCNGG 1 cut(s) 291
BmgT120I GGNCC 2 cut(s) 44, 114
BmrFI CCNGG 1 cut(s) 291
BmsI GCATC 1 cut(s) 205
BpiI GAAGAC 1 cut(s) 597
Bpu1102I GCTNAGC 3 cut(s) 561, 673, 703
BpuEI CTTGAG 1 cut(s) 650
BpuMI CCSGG 1 cut(s) 291
BsaJI CCNNGG 3 cut(s) 47, 57, 253
BsaWI WCCGGW 1 cut(s) 165
Bsc4I CCNNNNNNNGG 1 cut(s) 491
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 1 cut(s) 326
Bse3DI GCAATG 1 cut(s) 550
BseDI CCNNGG 3 cut(s) 47, 57, 253
BseGI GGATG 1 cut(s) 480
BseLI CCNNNNNNNGG 1 cut(s) 491
BseMI GCAATG 1 cut(s) 550
BseMII CTCAG 4 cut(s) 462, 552, 564, 687
BseNI ACTGG 1 cut(s) 326
BseRI GAGGAG 3 cut(s) 74, 77, 707
BseXI GCAGC 3 cut(s) 131, 143, 290
BsgI GTGCAG 1 cut(s) 227
BshFI GGCC 5 cut(s) 46, 99, 116, 135, 204
BsiSI CCGG 3 cut(s) 132, 166, 290
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 1 cut(s) 491
BsmFI GGGAC 1 cut(s) 104
BsnI GGCC 5 cut(s) 46, 99, 116, 135, 204
Bsp143I GATC 2 cut(s) 309, 364
Bsp1720I GCTNAGC 3 cut(s) 561, 673, 703
Bsp19I CCATGG 1 cut(s) 47
BspACI CCGC 7 cut(s) 112, 139, 142, 147, 160, 184, 250
BspANI GGCC 5 cut(s) 46, 99, 116, 135, 204
BspCNI CTCAG 4 cut(s) 461, 553, 563, 686
BspHI TCATGA 1 cut(s) 376
BspMI ACCTGC 2 cut(s) 249, 448
BspPI GGATC 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 550
BsrFI RCCGGY 1 cut(s) 131
BsrI ACTGG 1 cut(s) 326
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 3 cut(s) 47, 57, 253
BssMI GATC 2 cut(s) 309, 364
BssT1I CCWWGG 2 cut(s) 47, 253
Bst4CI ACNGT 4 cut(s) 509, 515, 581, 722
BstAPI GCANNNNNTGC 1 cut(s) 156
BstC8I GCNNGC 7 cut(s) 8, 114, 129, 133, 246, 250, 558
BstDEI CTNAG 5 cut(s) 448, 550, 561, 673, 703
BstDSI CCRYGG 1 cut(s) 47
BstF5I GGATG 1 cut(s) 480
BstKTI GATC 2 cut(s) 312, 367
BstMBI GATC 2 cut(s) 309, 364
BstMWI GCNNNNNNNGC 5 cut(s) 141, 147, 153, 156, 562
BstSCI CCNGG 1 cut(s) 289
BstV1I GCAGC 3 cut(s) 131, 143, 290
BstV2I GAAGAC 1 cut(s) 597
BsuRI GGCC 5 cut(s) 46, 99, 116, 135, 204
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 1 cut(s) 480
BtsIMutI CAGTG 3 cut(s) 319, 514, 586
BveI ACCTGC 2 cut(s) 249, 448
Cac8I GCNNGC 7 cut(s) 8, 114, 129, 133, 246, 250, 558
CciI TCATGA 1 cut(s) 376
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 44, 114
CviAII CATG 8 cut(s) 48, 95, 377, 454, 491, 642, 708, 715
DdeI CTNAG 5 cut(s) 448, 550, 561, 673, 703
DpnI GATC 2 cut(s) 311, 366
DpnII GATC 2 cut(s) 309, 364
EaeI YGGCCR 1 cut(s) 97
EciI GGCGGA 2 cut(s) 128, 173
Eco130I CCWWGG 2 cut(s) 47, 253
Eco147I AGGCCT 1 cut(s) 204
Eco57I CTGAAG 1 cut(s) 240
EcoT14I CCWWGG 2 cut(s) 47, 253
ErhI CCWWGG 2 cut(s) 47, 253
FaeI CATG 8 cut(s) 51, 98, 380, 457, 494, 645, 711, 718
FaiI YATR 9 cut(s) 49, 96, 378, 455, 492, 611, 643, 709, 716
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FaqI GGGAC 1 cut(s) 104
FatI CATG 8 cut(s) 47, 94, 376, 453, 490, 641, 707, 714
FauI CCCGC 2 cut(s) 105, 257
Fnu4HI GCNGC 6 cut(s) 142, 145, 148, 157, 160, 279
FokI GGATG 1 cut(s) 467
Fsp4HI GCNGC 6 cut(s) 142, 145, 148, 157, 160, 279
GluI GCNGC 6 cut(s) 142, 145, 148, 157, 160, 279
HaeIII GGCC 5 cut(s) 46, 99, 116, 135, 204
HapII CCGG 3 cut(s) 132, 166, 290
Hin1II CATG 8 cut(s) 51, 98, 380, 457, 494, 645, 711, 718
HindIII AAGCTT 3 cut(s) 26, 125, 344
HinfI GANTC 3 cut(s) 316, 495, 548
HpaII CCGG 3 cut(s) 132, 166, 290
HphI GGTGA 3 cut(s) 88, 391, 613
Hpy188I TCNGA 4 cut(s) 451, 553, 619, 664
Hpy188III TCNNGA 5 cut(s) 313, 337, 352, 377, 629
HpyCH4III ACNGT 4 cut(s) 509, 515, 581, 722
HpyCH4V TGCA 3 cut(s) 244, 278, 443
HpyF10VI GCNNNNNNNGC 5 cut(s) 141, 147, 153, 156, 562
HpyF3I CTNAG 5 cut(s) 448, 550, 561, 673, 703
Hsp92II CATG 8 cut(s) 51, 98, 380, 457, 494, 645, 711, 718
KroI GCCGGC 1 cut(s) 131
KroNI GCCGGC 1 cut(s) 133
Kzo9I GATC 2 cut(s) 309, 364
LpnPI CCDG 8 cut(s) 145, 179, 254, 303, 307, 453, 498, 570
Lsp1109I GCAGC 3 cut(s) 131, 143, 290
LweI GCATC 1 cut(s) 205
MaeIII GTNAC 3 cut(s) 397, 509, 515
MalI GATC 2 cut(s) 311, 366
MboI GATC 2 cut(s) 309, 364
MboII GAAGA 7 cut(s) 49, 233, 306, 352, 599, 602, 752
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 7 cut(s) 71, 79, 175, 273, 369, 532, 574
MluNI TGGCCA 1 cut(s) 99
MlyI GAGTC 1 cut(s) 542
MmeI TCCRAC 1 cut(s) 710
Mox20I TGGCCA 1 cut(s) 99
MroNI GCCGGC 1 cut(s) 131
MscI TGGCCA 1 cut(s) 99
MseI TTAA 3 cut(s) 174, 210, 638
Msp20I TGGCCA 1 cut(s) 99
MspA1I CMGCKG 3 cut(s) 144, 560, 677
MspI CCGG 3 cut(s) 132, 166, 290
MspR9I CCNGG 1 cut(s) 291
MwoI GCNNNNNNNGC 5 cut(s) 141, 147, 153, 156, 562
NaeI GCCGGC 1 cut(s) 133
NciI CCSGG 1 cut(s) 291
NcoI CCATGG 1 cut(s) 47
NdeII GATC 2 cut(s) 309, 364
NgoMIV GCCGGC 1 cut(s) 131
NlaIII CATG 8 cut(s) 51, 98, 380, 457, 494, 645, 711, 718
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 2 cut(s) 397, 509
PagI TCATGA 1 cut(s) 376
PceI AGGCCT 1 cut(s) 204
PdiI GCCGGC 1 cut(s) 133
PfeI GAWTC 2 cut(s) 316, 495
PflFI GACNNNGTC 1 cut(s) 658
PflMI CCANNNNNTGG 1 cut(s) 491
PkrI GCNGC 6 cut(s) 143, 146, 149, 158, 161, 280
PleI GAGTC 1 cut(s) 542
PpsI GAGTC 1 cut(s) 542
PshBI ATTAAT 1 cut(s) 174
PspPI GGNCC 2 cut(s) 44, 114
PsyI GACNNNGTC 1 cut(s) 658
PvuII CAGCTG 2 cut(s) 560, 677
SaqAI TTAA 3 cut(s) 174, 210, 638
SatI GCNGC 6 cut(s) 142, 145, 148, 157, 160, 279
Sau3AI GATC 2 cut(s) 309, 364
Sau96I GGNCC 2 cut(s) 44, 114
SchI GAGTC 1 cut(s) 542
ScrFI CCNGG 1 cut(s) 291
SfaNI GCATC 1 cut(s) 205
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
Sse9I AATT 7 cut(s) 71, 79, 175, 273, 369, 532, 574
SseBI AGGCCT 1 cut(s) 204
SsiI CCGC 7 cut(s) 112, 139, 142, 147, 160, 184, 250
StuI AGGCCT 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 2 cut(s) 47, 253
TaaI ACNGT 4 cut(s) 509, 515, 581, 722
TaqI TCGA 3 cut(s) 269, 653, 747
TasI AATT 7 cut(s) 71, 79, 175, 273, 369, 532, 574
TauI GCSGC 3 cut(s) 144, 150, 162
TfiI GAWTC 2 cut(s) 316, 495
Tru1I TTAA 3 cut(s) 174, 210, 638
Tru9I TTAA 3 cut(s) 174, 210, 638
TscAI CASTG 3 cut(s) 326, 514, 586
TseFI GTSAC 2 cut(s) 397, 509
TseI GCWGC 3 cut(s) 144, 156, 278
Tsp45I GTSAC 2 cut(s) 397, 509
TspDTI ATGAA 3 cut(s) 485, 598, 703
TspGWI ACGGA 1 cut(s) 704
TspRI CASTG 3 cut(s) 326, 514, 586
Tth111I GACNNNGTC 1 cut(s) 658
Van91I CCANNNNNTGG 1 cut(s) 491
VspI ATTAAT 1 cut(s) 174
XapI RAATTY 2 cut(s) 79, 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.