Rroxscaffold_4G00283220
MYB Family

Myb-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005146
Physical Location & Seq
Reverse (-)
5193467 .. 5194573
1107 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00283220.1

Sequence Viewer

Length: 750 bp
ATGGGCAGGCAACCTTGTTGTGACAAGCTTGGCGTCAAGAAAGGTCCGTGGACCGCCGAGGAGGACAAGAAACTGGTGAACTTCATCCTCACTCATGGCCAATGCTGCTGGCGCGCCGTCCCCAAGCTCGCGGGTCTGCGCCGCTGCGGCAAGAGCTGCCGCCTCCGCTGGACTAACTATCTCCGGCCTGACTTGAAGCGAGGGCTACTCAATGAAGCTGAGGAGCAGCTTGTTATTGACCTCCATGCTAGTCTTGGCAACAGGTGGTCTAAAATTGCAGCAAGATTGCCTGGAAGAACCGACAACGAGATCAAGAATCACTGGAATACACACATCAAGAAAAAACTTATCAAGATGGGGATTGATCCAATTACTCATGCGCCTCTCCAAAAACAAGTGACTACAACCCCACAGGAAACGCCTTGTGAAGCAGATATTCAACAAAGTGTGAACATCCCGGAGCATGGAGTCTCCGGCAACGAAAATGGCAGCTCTGCAACTGAGAATTCCTCGAGCAATGAAGCTCATTTACTAATTGAGGTCAATGCTAATGGTGACGACGACCCTTTGGTGAGTTACATATTATCCGATACGTTTCTTCAGGATTGGTCATGGAATTTTCCAGCTGTGGAAGACAATTACAGTGATCTTGGCTTGTCTTCAACTTCATCAGAGGATAGCTCAGCTTGGTTTATGGATTGCAAGGATTTTGGGGTTGAGGATTTCGAGCTTGGAAATTGTATCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

249

Amino Acids

27.87

Weight (kDa)

5.4

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 1.7e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 3.3e-09 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 72 - 111 2e-15 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 72 - 115 5.9e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 114, 131
AciI CCGC 6 cut(s) 54, 131, 142, 147, 160, 166
AclWI GGATC 1 cut(s) 359
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 2 cut(s) 505, 616
AcuI CTGAAG 1 cut(s) 584
AcyI GRCGYC 1 cut(s) 33
AfiI CCNNNNNNNGG 1 cut(s) 464
AgsI TTSAA 3 cut(s) 196, 440, 663
AjnI CCWGG 1 cut(s) 289
Alw26I GTCTC 1 cut(s) 475
AlwI GGATC 1 cut(s) 359
Ama87I CYCGRG 1 cut(s) 511
AoxI GGCC 2 cut(s) 97, 185
ApeKI GCWGC 6 cut(s) 105, 144, 156, 226, 278, 489
ApoI RAATTY 2 cut(s) 505, 616
AscI GGCGCGCC 1 cut(s) 112
AspLEI GCGC 4 cut(s) 114, 116, 141, 382
AspS9I GGNCC 2 cut(s) 44, 51
AsuC2I CCSGG 1 cut(s) 458
AsuHPI GGTGA 3 cut(s) 88, 566, 583
AvaI CYCGRG 1 cut(s) 511
AvaII GGWCC 2 cut(s) 44, 51
BalI TGGCCA 1 cut(s) 99
BarI GAAGNNNNNNTAC 2 cut(s) 513, 545
BbsI GAAGAC 2 cut(s) 639, 651
BbvCI CCTCAGC 1 cut(s) 219
BbvI GCAGC 6 cut(s) 92, 131, 143, 238, 290, 501
BccI CCATC 1 cut(s) 349
BceAI ACGGC 1 cut(s) 101
BciT130I CCWGG 1 cut(s) 291
BcnI CCSGG 1 cut(s) 458
BcoDI GTCTC 1 cut(s) 475
BfaI CTAG 1 cut(s) 249
BglI GCCNNNNNGGC 1 cut(s) 147
BisI GCNGC 9 cut(s) 106, 142, 145, 148, 157, 160, 227, 279, 490
BlpI GCTNAGC 1 cut(s) 682
BlsI GCNGC 9 cut(s) 107, 143, 146, 149, 158, 161, 228, 280, 491
Bme1390I CCNGG 2 cut(s) 291, 458
Bme18I GGWCC 2 cut(s) 44, 51
BmeT110I CYCGRG 1 cut(s) 511
BmgT120I GGNCC 2 cut(s) 44, 51
BmrFI CCNGG 2 cut(s) 291, 458
BpiI GAAGAC 2 cut(s) 639, 651
BplI GAGNNNNNCTC 6 cut(s) 192, 224, 494, 526, 665, 697
Bpu10I CCTNAGC 1 cut(s) 219
Bpu1102I GCTNAGC 1 cut(s) 682
BpuMI CCSGG 1 cut(s) 458
BsaHI GRCGYC 1 cut(s) 33
BsaJI CCNNGG 2 cut(s) 47, 57
Bsc4I CCNNNNNNNGG 1 cut(s) 464
Bse1I ACTGG 2 cut(s) 78, 326
Bse3DI GCAATG 1 cut(s) 523
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 47, 57
BseGI GGATG 2 cut(s) 84, 453
BseLI CCNNNNNNNGG 1 cut(s) 464
BseMI GCAATG 1 cut(s) 523
BseMII CTCAG 3 cut(s) 210, 492, 696
BseNI ACTGG 2 cut(s) 78, 326
BsePI GCGCGC 1 cut(s) 112
BseRI GAGGAG 2 cut(s) 74, 236
BseXI GCAGC 6 cut(s) 92, 131, 143, 238, 290, 501
Bsh1236I CGCG 2 cut(s) 114, 131
BshFI GGCC 2 cut(s) 99, 187
BsiHKCI CYCGRG 1 cut(s) 511
BsiSI CCGG 3 cut(s) 184, 458, 474
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 1 cut(s) 464
BsmAI GTCTC 1 cut(s) 475
BsmFI GGGAC 1 cut(s) 104
BsnI GGCC 2 cut(s) 99, 187
BsoBI CYCGRG 1 cut(s) 511
Bsp143I GATC 3 cut(s) 309, 364, 646
Bsp1720I GCTNAGC 1 cut(s) 682
BspACI CCGC 6 cut(s) 54, 131, 142, 147, 160, 166
BspANI GGCC 2 cut(s) 99, 187
BspCNI CTCAG 3 cut(s) 211, 493, 695
BspFNI CGCG 2 cut(s) 114, 131
BspPI GGATC 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 523
BsrI ACTGG 2 cut(s) 78, 326
BssECI CCNNGG 2 cut(s) 47, 57
BssHII GCGCGC 1 cut(s) 112
BssMI GATC 3 cut(s) 309, 364, 646
BssNI GRCGYC 1 cut(s) 33
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 1 cut(s) 644
BstACI GRCGYC 1 cut(s) 33
BstAPI GCANNNNNTGC 1 cut(s) 156
BstC8I GCNNGC 4 cut(s) 8, 110, 114, 129
BstDEI CTNAG 3 cut(s) 219, 501, 682
BstDSI CCRYGG 1 cut(s) 47
BstF5I GGATG 2 cut(s) 84, 453
BstFNI CGCG 2 cut(s) 114, 131
BstHHI GCGC 4 cut(s) 114, 116, 141, 382
BstKTI GATC 3 cut(s) 312, 367, 649
BstMAI GTCTC 1 cut(s) 475
BstMBI GATC 3 cut(s) 309, 364, 646
BstMWI GCNNNNNNNGC 6 cut(s) 105, 111, 147, 153, 156, 165
BstNI CCWGG 1 cut(s) 291
BstSCI CCNGG 2 cut(s) 289, 456
BstUI CGCG 2 cut(s) 114, 131
BstV1I GCAGC 6 cut(s) 92, 131, 143, 238, 290, 501
BstV2I GAAGAC 2 cut(s) 639, 651
BsuRI GGCC 2 cut(s) 99, 187
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 2 cut(s) 84, 453
BtsIMutI CAGTG 2 cut(s) 319, 649
Cac8I GCNNGC 4 cut(s) 8, 110, 114, 129
CfoI GCGC 4 cut(s) 114, 116, 141, 382
Cfr13I GGNCC 2 cut(s) 44, 51
CseI GACGC 1 cut(s) 22
CviAII CATG 5 cut(s) 95, 245, 377, 464, 612
DdeI CTNAG 3 cut(s) 219, 501, 682
DpnI GATC 3 cut(s) 311, 366, 648
DpnII GATC 3 cut(s) 309, 364, 646
EaeI YGGCCR 1 cut(s) 97
Eco47I GGWCC 2 cut(s) 44, 51
Eco57I CTGAAG 1 cut(s) 584
Eco88I CYCGRG 1 cut(s) 511
EcoRI GAATTC 1 cut(s) 505
EcoRII CCWGG 1 cut(s) 289
FaeI CATG 5 cut(s) 98, 248, 380, 467, 615
FaiI YATR 7 cut(s) 96, 246, 378, 465, 581, 613, 695
FaqI GGGAC 1 cut(s) 104
FatI CATG 5 cut(s) 94, 244, 376, 463, 611
FauI CCCGC 1 cut(s) 124
Fnu4HI GCNGC 9 cut(s) 106, 142, 145, 148, 157, 160, 227, 279, 490
FokI GGATG 2 cut(s) 71, 440
Fsp4HI GCNGC 9 cut(s) 106, 142, 145, 148, 157, 160, 227, 279, 490
FspBI CTAG 1 cut(s) 249
GlaI GCGC 4 cut(s) 113, 115, 140, 381
GluI GCNGC 9 cut(s) 106, 142, 145, 148, 157, 160, 227, 279, 490
HaeIII GGCC 2 cut(s) 99, 187
HapII CCGG 3 cut(s) 184, 458, 474
HgaI GACGC 1 cut(s) 22
HhaI GCGC 4 cut(s) 114, 116, 141, 382
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 5 cut(s) 98, 248, 380, 467, 615
Hin6I GCGC 4 cut(s) 112, 114, 139, 380
HinP1I GCGC 4 cut(s) 112, 114, 139, 380
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 2 cut(s) 316, 468
HpaII CCGG 3 cut(s) 184, 458, 474
HphI GGTGA 3 cut(s) 88, 566, 583
Hpy166II GTNNAC 3 cut(s) 51, 79, 451
Hpy188I TCNGA 2 cut(s) 589, 673
Hpy188III TCNNGA 5 cut(s) 37, 313, 337, 352, 602
Hpy8I GTNNAC 3 cut(s) 51, 79, 451
Hpy99I CGWCG 1 cut(s) 563
HpyCH4III ACNGT 1 cut(s) 644
HpyCH4IV ACGT 1 cut(s) 593
HpyCH4V TGCA 3 cut(s) 278, 497, 702
HpyF10VI GCNNNNNNNGC 6 cut(s) 105, 111, 147, 153, 156, 165
HpyF3I CTNAG 3 cut(s) 219, 501, 682
HpySE526I ACGT 1 cut(s) 593
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 5 cut(s) 98, 248, 380, 467, 615
HspAI GCGC 4 cut(s) 112, 114, 139, 380
Kzo9I GATC 3 cut(s) 309, 364, 646
LmnI GCTCC 2 cut(s) 223, 460
Lsp1109I GCAGC 6 cut(s) 92, 131, 143, 238, 290, 501
MaeI CTAG 1 cut(s) 249
MaeII ACGT 1 cut(s) 593
MaeIII GTNAC 4 cut(s) 20, 397, 554, 575
MalI GATC 3 cut(s) 311, 366, 648
MboI GATC 3 cut(s) 309, 364, 646
MboII GAAGA 4 cut(s) 306, 590, 644, 651
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 8 cut(s) 273, 369, 505, 534, 616, 637, 736, 745
MluNI TGGCCA 1 cut(s) 99
MlyI GAGTC 1 cut(s) 477
Mox20I TGGCCA 1 cut(s) 99
MscI TGGCCA 1 cut(s) 99
MseI TTAA 1 cut(s) 748
Msp20I TGGCCA 1 cut(s) 99
MspA1I CMGCKG 3 cut(s) 144, 168, 626
MspI CCGG 3 cut(s) 184, 458, 474
MspR9I CCNGG 2 cut(s) 291, 458
MvaI CCWGG 1 cut(s) 291
MvnI CGCG 2 cut(s) 114, 131
MwoI GCNNNNNNNGC 6 cut(s) 105, 111, 147, 153, 156, 165
NciI CCSGG 1 cut(s) 458
NdeII GATC 3 cut(s) 309, 364, 646
NlaIII CATG 5 cut(s) 98, 248, 380, 467, 615
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 3 cut(s) 20, 397, 554
PaeR7I CTCGAG 1 cut(s) 511
PalAI GGCGCGCC 1 cut(s) 112
PauI GCGCGC 1 cut(s) 112
PfeI GAWTC 1 cut(s) 316
PfoI TCCNGGA 1 cut(s) 456
PkrI GCNGC 9 cut(s) 107, 143, 146, 149, 158, 161, 228, 280, 491
PleI GAGTC 1 cut(s) 476
PpsI GAGTC 1 cut(s) 476
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspPI GGNCC 2 cut(s) 44, 51
PspXI VCTCGAGB 1 cut(s) 511
PteI GCGCGC 1 cut(s) 112
PvuII CAGCTG 1 cut(s) 626
SaqAI TTAA 1 cut(s) 748
SatI GCNGC 9 cut(s) 106, 142, 145, 148, 157, 160, 227, 279, 490
Sau3AI GATC 3 cut(s) 309, 364, 646
Sau96I GGNCC 2 cut(s) 44, 51
SchI GAGTC 1 cut(s) 477
ScrFI CCNGG 2 cut(s) 291, 458
Sfr274I CTCGAG 1 cut(s) 511
SgsI GGCGCGCC 1 cut(s) 112
SinI GGWCC 2 cut(s) 44, 51
SlaI CTCGAG 1 cut(s) 511
SmlI CTYRAG 1 cut(s) 511
SmoI CTYRAG 1 cut(s) 511
Sse9I AATT 8 cut(s) 273, 369, 505, 534, 616, 637, 736, 745
SsiI CCGC 6 cut(s) 54, 131, 142, 147, 160, 166
SspMI CTAG 1 cut(s) 249
StyD4I CCNGG 2 cut(s) 289, 456
TaaI ACNGT 1 cut(s) 644
TaiI ACGT 1 cut(s) 596
TaqI TCGA 2 cut(s) 512, 726
TasI AATT 8 cut(s) 273, 369, 505, 534, 616, 637, 736, 745
TauI GCSGC 3 cut(s) 144, 150, 162
TfiI GAWTC 1 cut(s) 316
Tru1I TTAA 1 cut(s) 748
Tru9I TTAA 1 cut(s) 748
TscAI CASTG 2 cut(s) 326, 649
TseFI GTSAC 3 cut(s) 20, 397, 554
TseI GCWGC 6 cut(s) 105, 144, 156, 226, 278, 489
Tsp45I GTSAC 3 cut(s) 20, 397, 554
TspDTI ATGAA 4 cut(s) 73, 228, 534, 657
TspGWI ACGGA 1 cut(s) 36
TspRI CASTG 2 cut(s) 326, 649
VpaK11BI GGWCC 2 cut(s) 44, 51
XapI RAATTY 2 cut(s) 505, 616
XcmI CCANNNNNNNNNTGG 1 cut(s) 251
XhoI CTCGAG 1 cut(s) 511
XspI CTAG 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.