MD07G1256200.v1.1
MYB Family

Myb-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005146
Physical Location & Seq
Forward (+)
32297236 .. 32298745
1510 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1256200.v1.1.491

Sequence Viewer

Length: 840 bp
ATGGGCAGGCAACCTTGTTGTGACAAGCTTGGAGTGAAGAAAGGGCCATGGACTGCCGAGGAGGATAAGAAACTGGTGAATTTTCTCCTCACGCATGGCCAATGTTGTTGGCGGGCTGTCCCCAAGCTCGCTGGCCTCCGTCGCTGCGGCAAGAGCTGCCGCCTCCGGTGGACTAATTACCTCCGCCCCGACTTGAAAAGAGGCCTCCTTAATGATGCTGAAGAACAACTTGTTATTGACCTCCATGCCCGCCTTGGCAACAGGTGGTCTAAAATTGCAGCAAGATTGCCTGGAAGAACTGACAATGAGATCAAGAACCATTGGAATACACACATCAAGAAAAAGCTTATCAAGATGGGGATTGATCCAATTACTCATGAGCCTCTCCACAAACAGGCCATCACCCCAGAAGAAATGCCTTGTGAAGCAAGCAATCAACCTGCAGGCTCAGACGTGAATATTCCGCAGAATATGGATATCCCAGAACATGAAGTCTATAGCAACAGTGAAGCAAGCAATCAACCTGCAGGCTTAGACATGAATATTCCGAGGAATATGAATATCCCAGAACATGAAATCTCTAGCAACAGTGATGGTGGTGGCTCTGCAAGTGAAAATTCCCCAAGCAATGAGTCTCAGTCAGCTGAGCCAAACCCTAATTACAGTGAGGAAGATGACCCATTGTTGAGTTTCATATTATCCGATACATTTCTCGAGGATTTAACATGGGACTTTTCGACCTCGTCAGAAGACGGCTCAGCTGATAATCCAACGGAGGAGAATAGCTTAGCATGGTTCATGGACTGTAAGGATTTTGGAGTTCAAGATTTTGAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

280

Amino Acids

31.3

Weight (kDa)

4.92

Isoelectric Point (pI)

53.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 3.9e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 1.3e-08 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 72 - 115 3.2e-07 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 73 - 111 1.8e-15 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 448, 532
AciI CCGC 6 cut(s) 112, 147, 160, 184, 250, 464
AclWI GGATC 1 cut(s) 359
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 2 cut(s) 79, 616
AcuI CTGAAG 1 cut(s) 240
AfiI CCNNNNNNNGG 1 cut(s) 394
AgsI TTSAA 2 cut(s) 196, 824
AjiI CACGTC 1 cut(s) 454
AjnI CCWGG 1 cut(s) 289
AluBI AGCT 8 cut(s) 28, 127, 156, 346, 644, 761, 786, 835
AluI AGCT 8 cut(s) 28, 127, 156, 346, 644, 761, 786, 835
Alw26I GTCTC 1 cut(s) 639
AlwI GGATC 1 cut(s) 359
Ama87I CYCGRG 1 cut(s) 713
AoxI GGCC 5 cut(s) 44, 97, 133, 202, 396
ApeKI GCWGC 3 cut(s) 144, 156, 278
ApoI RAATTY 2 cut(s) 79, 616
AspS9I GGNCC 1 cut(s) 44
AsuHPI GGTGA 2 cut(s) 88, 394
AvaI CYCGRG 1 cut(s) 713
BalI TGGCCA 1 cut(s) 99
BbsI GAAGAC 1 cut(s) 756
BbvI GCAGC 3 cut(s) 131, 143, 290
BccI CCATC 3 cut(s) 349, 407, 587
BceAI ACGGC 1 cut(s) 769
BciT130I CCWGG 1 cut(s) 291
BcoDI GTCTC 1 cut(s) 639
BfaI CTAG 1 cut(s) 582
BfmI CTRYAG 3 cut(s) 441, 496, 525
BfuAI ACCTGC 2 cut(s) 448, 532
BisI GCNGC 5 cut(s) 145, 148, 157, 160, 279
BlpI GCTNAGC 3 cut(s) 645, 757, 787
BlsI GCNGC 5 cut(s) 146, 149, 158, 161, 280
Bme1390I CCNGG 1 cut(s) 291
BmeT110I CYCGRG 1 cut(s) 713
BmgBI CACGTC 1 cut(s) 454
BmgT120I GGNCC 1 cut(s) 44
BmrFI CCNGG 1 cut(s) 291
BmsI GCATC 1 cut(s) 205
BpiI GAAGAC 1 cut(s) 756
Bpu1102I GCTNAGC 3 cut(s) 645, 757, 787
BsaJI CCNNGG 4 cut(s) 47, 57, 253, 548
BsaWI WCCGGW 1 cut(s) 165
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse1I ACTGG 1 cut(s) 78
Bse3DI GCAATG 1 cut(s) 634
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 4 cut(s) 47, 57, 253, 548
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMI GCAATG 1 cut(s) 634
BseMII CTCAG 4 cut(s) 462, 636, 650, 771
BseNI ACTGG 1 cut(s) 78
BseRI GAGGAG 3 cut(s) 74, 77, 791
BseXI GCAGC 3 cut(s) 131, 143, 290
BshFI GGCC 5 cut(s) 46, 99, 135, 204, 398
BsiHKCI CYCGRG 1 cut(s) 713
BsiSI CCGG 1 cut(s) 166
BslFI GGGAC 2 cut(s) 104, 743
BslI CCNNNNNNNGG 1 cut(s) 394
BsmAI GTCTC 1 cut(s) 639
BsmFI GGGAC 2 cut(s) 104, 743
BsnI GGCC 5 cut(s) 46, 99, 135, 204, 398
BsoBI CYCGRG 1 cut(s) 713
Bsp143I GATC 2 cut(s) 309, 364
Bsp1720I GCTNAGC 3 cut(s) 645, 757, 787
Bsp19I CCATGG 1 cut(s) 47
BspACI CCGC 6 cut(s) 112, 147, 160, 184, 250, 464
BspANI GGCC 5 cut(s) 46, 99, 135, 204, 398
BspCNI CTCAG 4 cut(s) 461, 637, 649, 770
BspHI TCATGA 1 cut(s) 376
BspMAI CTGCAG 2 cut(s) 445, 529
BspMI ACCTGC 2 cut(s) 448, 532
BspPI GGATC 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 634
BsrI ACTGG 1 cut(s) 78
BssECI CCNNGG 4 cut(s) 47, 57, 253, 548
BssMI GATC 2 cut(s) 309, 364
BssT1I CCWWGG 2 cut(s) 47, 253
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 4 cut(s) 506, 590, 665, 806
BstAPI GCANNNNNTGC 1 cut(s) 156
BstC8I GCNNGC 9 cut(s) 8, 114, 129, 133, 250, 430, 445, 514, 529
BstDEI CTNAG 6 cut(s) 448, 532, 636, 645, 757, 787
BstDSI CCRYGG 1 cut(s) 47
BstKTI GATC 2 cut(s) 312, 367
BstMAI GTCTC 1 cut(s) 639
BstMBI GATC 2 cut(s) 309, 364
BstMWI GCNNNNNNNGC 3 cut(s) 141, 153, 156
BstNI CCWGG 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 289
BstSFI CTRYAG 3 cut(s) 441, 496, 525
BstV1I GCAGC 3 cut(s) 131, 143, 290
BstV2I GAAGAC 1 cut(s) 756
BsuRI GGCC 5 cut(s) 46, 99, 135, 204, 398
BtgI CCRYGG 1 cut(s) 47
BtrI CACGTC 1 cut(s) 454
BtsIMutI CAGTG 3 cut(s) 511, 595, 670
BveI ACCTGC 2 cut(s) 448, 532
Cac8I GCNNGC 9 cut(s) 8, 114, 129, 133, 250, 430, 445, 514, 529
CciI TCATGA 1 cut(s) 376
Cfr13I GGNCC 1 cut(s) 44
DdeI CTNAG 6 cut(s) 448, 532, 636, 645, 757, 787
DpnI GATC 2 cut(s) 311, 366
DpnII GATC 2 cut(s) 309, 364
EaeI YGGCCR 1 cut(s) 97
EciI GGCGGA 1 cut(s) 173
Eco130I CCWWGG 2 cut(s) 47, 253
Eco147I AGGCCT 1 cut(s) 204
Eco32I GATATC 1 cut(s) 478
Eco57I CTGAAG 1 cut(s) 240
Eco88I CYCGRG 1 cut(s) 713
EcoRII CCWGG 1 cut(s) 289
EcoRV GATATC 1 cut(s) 478
EcoT14I CCWWGG 2 cut(s) 47, 253
ErhI CCWWGG 2 cut(s) 47, 253
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FaqI GGGAC 2 cut(s) 104, 743
FauI CCCGC 2 cut(s) 105, 257
Fnu4HI GCNGC 5 cut(s) 145, 148, 157, 160, 279
Fsp4HI GCNGC 5 cut(s) 145, 148, 157, 160, 279
FspBI CTAG 1 cut(s) 582
GluI GCNGC 5 cut(s) 145, 148, 157, 160, 279
HaeIII GGCC 5 cut(s) 46, 99, 135, 204, 398
HapII CCGG 1 cut(s) 166
HindIII AAGCTT 2 cut(s) 26, 344
HinfI GANTC 1 cut(s) 632
HpaII CCGG 1 cut(s) 166
HphI GGTGA 2 cut(s) 88, 394
Hpy166II GTNNAC 1 cut(s) 171
Hpy188I TCNGA 4 cut(s) 451, 549, 703, 748
Hpy188III TCNNGA 6 cut(s) 313, 337, 352, 377, 713, 824
Hpy8I GTNNAC 1 cut(s) 171
Hpy99I CGWCG 1 cut(s) 144
HpyCH4III ACNGT 4 cut(s) 506, 590, 665, 806
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 4 cut(s) 278, 443, 527, 608
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 153, 156
HpyF3I CTNAG 6 cut(s) 448, 532, 636, 645, 757, 787
HpySE526I ACGT 1 cut(s) 453
Kzo9I GATC 2 cut(s) 309, 364
Lsp1109I GCAGC 3 cut(s) 131, 143, 290
LweI GCATC 1 cut(s) 205
MaeI CTAG 1 cut(s) 582
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 1 cut(s) 20
MalI GATC 2 cut(s) 311, 366
MboI GATC 2 cut(s) 309, 364
MboII GAAGA 6 cut(s) 49, 233, 306, 422, 683, 761
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 6 cut(s) 79, 175, 273, 369, 616, 658
MluNI TGGCCA 1 cut(s) 99
MlyI GAGTC 1 cut(s) 641
MmeI TCCRAC 1 cut(s) 794
Mox20I TGGCCA 1 cut(s) 99
MscI TGGCCA 1 cut(s) 99
MseI TTAA 2 cut(s) 210, 722
Msp20I TGGCCA 1 cut(s) 99
MspA1I CMGCKG 2 cut(s) 644, 761
MspI CCGG 1 cut(s) 166
MspR9I CCNGG 1 cut(s) 291
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 3 cut(s) 141, 153, 156
NcoI CCATGG 1 cut(s) 47
NdeII GATC 2 cut(s) 309, 364
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 1 cut(s) 20
PaeR7I CTCGAG 1 cut(s) 713
PagI TCATGA 1 cut(s) 376
PceI AGGCCT 1 cut(s) 204
PflFI GACNNNGTC 1 cut(s) 742
PkrI GCNGC 5 cut(s) 146, 149, 158, 161, 280
PleI GAGTC 1 cut(s) 640
PpsI GAGTC 1 cut(s) 640
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspPI GGNCC 1 cut(s) 44
PstI CTGCAG 2 cut(s) 445, 529
PsyI GACNNNGTC 1 cut(s) 742
PvuII CAGCTG 2 cut(s) 644, 761
SaqAI TTAA 2 cut(s) 210, 722
SatI GCNGC 5 cut(s) 145, 148, 157, 160, 279
Sau3AI GATC 2 cut(s) 309, 364
Sau96I GGNCC 1 cut(s) 44
SbfI CCTGCAGG 2 cut(s) 445, 529
SchI GAGTC 1 cut(s) 641
ScrFI CCNGG 1 cut(s) 291
SdaI CCTGCAGG 2 cut(s) 445, 529
SfaNI GCATC 1 cut(s) 205
SfcI CTRYAG 3 cut(s) 441, 496, 525
Sfr274I CTCGAG 1 cut(s) 713
SlaI CTCGAG 1 cut(s) 713
SmlI CTYRAG 1 cut(s) 713
SmoI CTYRAG 1 cut(s) 713
Sse8387I CCTGCAGG 2 cut(s) 445, 529
Sse9I AATT 6 cut(s) 79, 175, 273, 369, 616, 658
SseBI AGGCCT 1 cut(s) 204
SsiI CCGC 6 cut(s) 112, 147, 160, 184, 250, 464
SspI AATATT 2 cut(s) 460, 544
SspMI CTAG 1 cut(s) 582
StuI AGGCCT 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 2 cut(s) 47, 253
TaaI ACNGT 4 cut(s) 506, 590, 665, 806
TaiI ACGT 1 cut(s) 456
TaqI TCGA 2 cut(s) 714, 737
TasI AATT 6 cut(s) 79, 175, 273, 369, 616, 658
TauI GCSGC 2 cut(s) 150, 162
Tru1I TTAA 2 cut(s) 210, 722
Tru9I TTAA 2 cut(s) 210, 722
TscAI CASTG 3 cut(s) 511, 595, 670
TseFI GTSAC 1 cut(s) 20
TseI GCWGC 3 cut(s) 144, 156, 278
Tsp45I GTSAC 1 cut(s) 20
TspDTI ATGAA 6 cut(s) 504, 554, 572, 588, 682, 787
TspGWI ACGGA 2 cut(s) 128, 788
TspRI CASTG 3 cut(s) 511, 595, 670
Tth111I GACNNNGTC 1 cut(s) 742
XapI RAATTY 2 cut(s) 79, 616
XcmI CCANNNNNNNNNTGG 1 cut(s) 251
XhoI CTCGAG 1 cut(s) 713
XspI CTAG 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.