Rroxscaffold_3G00254920
MYB Family

Myb-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005146
Physical Location & Seq
Forward (+)
49221860 .. 49223984
2125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00254920.1

Sequence Viewer

Length: 636 bp
ATGGGAAGGCAACCTTGCTGTGACAAACTTGGGGTTAAGAAAGGGCCATGGACAGCCGAGGAAGATAAGAAGCTCATCAACTTCATTCTCACAAATGGCCAGTGTTGCTGGAGAGCCGTCCCCAAGCTCGCTGGGCTTCGCCGCTGTGGCAAGAGTTGCAGACTCCGTTGGACCAACTATCTCCGTCCCGACTTGAAGAGAGGCCTCCTTACAGAATCTGAAGAACAATTGGTCATTGATCTCCATGCTCGTCTCGGAAATAGGTGGTCCAAGATTGCAGCCAGATTGCCAGGGAGGACTGATAATGAAATCAAGAATCACTGGAACACCCACATCAAGAAAAAGCTGCTTAGAATGGGAATTGATCCCGTCACGCATGAACCCCTCCACAAAGATCAAGATGACTTGATCAACAAGGAAACTATTATTTCATCCCATACTAATCAAGCTAACAATAACTTGCCTCAATCTACAACTGAAAATTCATCTTCAAACTCATCACCAGCTGAGAATTCATCATCAAGCTCATCACCAAATGAAAATTCTTCTGGAGACGACCAATCGACTTTAATTGATAGCATTTGCAACGTTGACGAGTCGTTGATGAACAGTCTTTGGGTGGATGAAACTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

211

Amino Acids

23.72

Weight (kDa)

7.62

Isoelectric Point (pI)

56.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 1.2e-15 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 3e-09 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 70 - 111 9.4e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 115 1.3e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 142
AclI AACGTT 1 cut(s) 588
AclWI GGATC 1 cut(s) 359
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 3 cut(s) 481, 511, 541
AcuI CTGAAG 1 cut(s) 240
AgsI TTSAA 2 cut(s) 196, 492
AjnI CCWGG 1 cut(s) 289
AjuI GAANNNNNNNTTGG 2 cut(s) 526, 558
AluBI AGCT 6 cut(s) 73, 127, 346, 449, 506, 525
AluI AGCT 6 cut(s) 73, 127, 346, 449, 506, 525
Alw26I GTCTC 2 cut(s) 257, 546
AlwI GGATC 1 cut(s) 359
AlwNI CAGNNNCTG 1 cut(s) 218
AoxI GGCC 3 cut(s) 44, 97, 202
ApeKI GCWGC 2 cut(s) 278, 346
ApoI RAATTY 3 cut(s) 481, 511, 541
AspS9I GGNCC 3 cut(s) 44, 171, 267
AsuHPI GGTGA 2 cut(s) 492, 522
AvaII GGWCC 2 cut(s) 171, 267
BalI TGGCCA 1 cut(s) 99
BbvI GCAGC 2 cut(s) 290, 333
BceAI ACGGC 1 cut(s) 101
BciT130I CCWGG 1 cut(s) 291
BclI TGATCA 1 cut(s) 408
BcoDI GTCTC 2 cut(s) 257, 546
BglI GCCNNNNNGGC 1 cut(s) 147
BisI GCNGC 3 cut(s) 142, 279, 347
BlsI GCNGC 3 cut(s) 143, 280, 348
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 2 cut(s) 171, 267
BmgT120I GGNCC 3 cut(s) 44, 171, 267
BmrFI CCNGG 1 cut(s) 291
BpmI CTGGAG 2 cut(s) 130, 570
BsaJI CCNNGG 3 cut(s) 47, 57, 290
Bse1I ACTGG 2 cut(s) 100, 326
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 3 cut(s) 47, 57, 290
BseGI GGATG 2 cut(s) 431, 628
BseMII CTCAG 1 cut(s) 498
BseNI ACTGG 2 cut(s) 100, 326
BseXI GCAGC 2 cut(s) 290, 333
BseYI CCCAGC 1 cut(s) 131
BshFI GGCC 3 cut(s) 46, 99, 204
BslFI GGGAC 2 cut(s) 104, 171
BsmAI GTCTC 2 cut(s) 257, 546
BsmBI CGTCTC 2 cut(s) 257, 546
BsmFI GGGAC 2 cut(s) 104, 171
BsnI GGCC 3 cut(s) 46, 99, 204
Bsp143I GATC 4 cut(s) 238, 364, 394, 408
Bsp19I CCATGG 1 cut(s) 47
BspACI CCGC 1 cut(s) 142
BspANI GGCC 3 cut(s) 46, 99, 204
BspCNI CTCAG 1 cut(s) 499
BspPI GGATC 1 cut(s) 359
BsrI ACTGG 2 cut(s) 100, 326
BssECI CCNNGG 3 cut(s) 47, 57, 290
BssMI GATC 4 cut(s) 238, 364, 394, 408
BssT1I CCWWGG 1 cut(s) 47
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 1 cut(s) 611
Bst6I CTCTTC 1 cut(s) 191
BstAPI GCANNNNNTGC 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 129
BstDEI CTNAG 2 cut(s) 350, 507
BstDSI CCRYGG 1 cut(s) 47
BstF5I GGATG 2 cut(s) 431, 628
BstKTI GATC 4 cut(s) 241, 367, 397, 411
BstMAI GTCTC 2 cut(s) 257, 546
BstMBI GATC 4 cut(s) 238, 364, 394, 408
BstMWI GCNNNNNNNGC 4 cut(s) 105, 133, 147, 156
BstNI CCWGG 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 289
BstV1I GCAGC 2 cut(s) 290, 333
BsuRI GGCC 3 cut(s) 46, 99, 204
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 2 cut(s) 431, 628
BtsIMutI CAGTG 2 cut(s) 107, 319
Cac8I GCNNGC 1 cut(s) 129
CaiI CAGNNNCTG 1 cut(s) 218
Cfr13I GGNCC 3 cut(s) 44, 171, 267
CviAII CATG 3 cut(s) 48, 245, 377
DdeI CTNAG 2 cut(s) 350, 507
DpnI GATC 4 cut(s) 240, 366, 396, 410
DpnII GATC 4 cut(s) 238, 364, 394, 408
EaeI YGGCCR 1 cut(s) 97
Eam1104I CTCTTC 1 cut(s) 191
EarI CTCTTC 1 cut(s) 191
Eco130I CCWWGG 1 cut(s) 47
Eco147I AGGCCT 1 cut(s) 204
Eco47I GGWCC 2 cut(s) 171, 267
Eco57I CTGAAG 1 cut(s) 240
EcoRI GAATTC 1 cut(s) 511
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 47
ErhI CCWWGG 1 cut(s) 47
Esp3I CGTCTC 2 cut(s) 257, 546
FaeI CATG 3 cut(s) 51, 248, 380
FaiI YATR 4 cut(s) 49, 246, 378, 438
FalI AAGNNNNNCTT 1 cut(s) 30
FaqI GGGAC 2 cut(s) 104, 171
FatI CATG 3 cut(s) 47, 244, 376
FbaI TGATCA 1 cut(s) 408
Fnu4HI GCNGC 3 cut(s) 142, 279, 347
FokI GGATG 1 cut(s) 418
Fsp4HI GCNGC 3 cut(s) 142, 279, 347
GluI GCNGC 3 cut(s) 142, 279, 347
GsaI CCCAGC 1 cut(s) 135
GsuI CTGGAG 2 cut(s) 130, 570
HaeIII GGCC 3 cut(s) 46, 99, 204
Hin1II CATG 3 cut(s) 51, 248, 380
HincII GTYRAC 1 cut(s) 592
HindII GTYRAC 1 cut(s) 592
HinfI GANTC 4 cut(s) 162, 215, 316, 596
HphI GGTGA 2 cut(s) 492, 522
Hpy166II GTNNAC 1 cut(s) 592
Hpy188I TCNGA 2 cut(s) 220, 257
Hpy188III TCNNGA 5 cut(s) 188, 313, 337, 398, 549
Hpy8I GTNNAC 1 cut(s) 592
HpyCH4III ACNGT 1 cut(s) 611
HpyCH4IV ACGT 1 cut(s) 588
HpyCH4V TGCA 3 cut(s) 159, 278, 585
HpyF10VI GCNNNNNNNGC 4 cut(s) 105, 133, 147, 156
HpyF3I CTNAG 2 cut(s) 350, 507
HpySE526I ACGT 1 cut(s) 588
Hsp92II CATG 3 cut(s) 51, 248, 380
Ksp22I TGATCA 1 cut(s) 408
Kzo9I GATC 4 cut(s) 238, 364, 394, 408
LpnPI CCDG 9 cut(s) 94, 113, 117, 276, 295, 303, 307, 516, 534
Lsp1109I GCAGC 2 cut(s) 290, 333
MaeII ACGT 1 cut(s) 588
MaeIII GTNAC 2 cut(s) 20, 370
MalI GATC 4 cut(s) 240, 366, 396, 410
MboI GATC 4 cut(s) 238, 364, 394, 408
MboII GAAGA 5 cut(s) 74, 208, 233, 480, 537
MfeI CAATTG 1 cut(s) 227
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 6 cut(s) 227, 360, 481, 511, 541, 570
MluNI TGGCCA 1 cut(s) 99
MlyI GAGTC 2 cut(s) 156, 605
MmeI TCCRAC 1 cut(s) 149
MnlI CCTC 6 cut(s) 52, 194, 215, 288, 395, 474
Mox20I TGGCCA 1 cut(s) 99
MscI TGGCCA 1 cut(s) 99
MseI TTAA 2 cut(s) 36, 569
Msp20I TGGCCA 1 cut(s) 99
MspA1I CMGCKG 2 cut(s) 144, 506
MspR9I CCNGG 1 cut(s) 291
MunI CAATTG 1 cut(s) 227
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 4 cut(s) 105, 133, 147, 156
NcoI CCATGG 1 cut(s) 47
NdeII GATC 4 cut(s) 238, 364, 394, 408
NlaIII CATG 3 cut(s) 51, 248, 380
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 2 cut(s) 20, 370
PceI AGGCCT 1 cut(s) 204
PfeI GAWTC 2 cut(s) 215, 316
PkrI GCNGC 3 cut(s) 143, 280, 348
PleI GAGTC 2 cut(s) 156, 604
PpsI GAGTC 2 cut(s) 156, 604
Psp1406I AACGTT 1 cut(s) 588
Psp6I CCWGG 1 cut(s) 289
PspFI CCCAGC 1 cut(s) 131
PspGI CCWGG 1 cut(s) 289
PspPI GGNCC 3 cut(s) 44, 171, 267
PstNI CAGNNNCTG 1 cut(s) 218
PvuII CAGCTG 1 cut(s) 506
SaqAI TTAA 2 cut(s) 36, 569
SatI GCNGC 3 cut(s) 142, 279, 347
Sau3AI GATC 4 cut(s) 238, 364, 394, 408
Sau96I GGNCC 3 cut(s) 44, 171, 267
SchI GAGTC 2 cut(s) 156, 605
ScrFI CCNGG 1 cut(s) 291
SetI ASST 9 cut(s) 16, 75, 129, 266, 348, 451, 508, 527, 591
SinI GGWCC 2 cut(s) 171, 267
Sse9I AATT 6 cut(s) 227, 360, 481, 511, 541, 570
SseBI AGGCCT 1 cut(s) 204
SsiI CCGC 1 cut(s) 142
StuI AGGCCT 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 1 cut(s) 47
TaaI ACNGT 1 cut(s) 611
TaiI ACGT 1 cut(s) 591
TaqI TCGA 1 cut(s) 563
TasI AATT 6 cut(s) 227, 360, 481, 511, 541, 570
TauI GCSGC 1 cut(s) 144
TfiI GAWTC 2 cut(s) 215, 316
Tru1I TTAA 2 cut(s) 36, 569
Tru9I TTAA 2 cut(s) 36, 569
TscAI CASTG 2 cut(s) 107, 326
TseFI GTSAC 2 cut(s) 20, 370
TseI GCWGC 2 cut(s) 278, 346
Tsp45I GTSAC 2 cut(s) 20, 370
TspDTI ATGAA 8 cut(s) 73, 321, 393, 420, 474, 504, 552, 620
TspGWI ACGGA 2 cut(s) 155, 173
TspRI CASTG 2 cut(s) 107, 326
VpaK11BI GGWCC 2 cut(s) 171, 267
XapI RAATTY 3 cut(s) 481, 511, 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.