MD04G1080600.v1.1
MYB Family

Myb-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005146
Physical Location & Seq
Reverse (-)
11085369 .. 11087517
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1080600.v1.1.491

Sequence Viewer

Length: 909 bp
ATGGGAAGGCAACCGTGTTGTGACAAACTTGGGGTAAAGAAAGGGCCATGGACTGCTGAGGAGGACAAGAAGCTCATCAGCTTTATTATCACCAATGGCCAGTGTTGTTGGAGGGCTGTGCCTAAACTCGCTGGCCTTCGCCGGTGTGGTAAGAGTTGCAGGCTCCGTTGGACGAATTATCTCCGCCCCGACTTGAAGAGAGGCCTTCTCACTGAAGCTGAAGAACAGTTGGTTATCGATCTCCACGCTCGTCTTGGAAATAGGTGGTCCAAGATTGCAGGCAGATTGCCAGGGAGAACAGATAATGAGATCAAGAATCATTGGAACACCCATATCAAGAAAAAGCTGCTTAGGATGGGAATTGATCCTGTCACACATGAACCCCTTCACAAAGAAGAAGAGTTGCCGTCCAATATTAAGGGAACTAACTCATCATCATCATCTTCCCATGATCATGATGATCATAACAATAATTTGCCAGCTGATCAGTCTACAAATGATCTTCACAATTCACAAGAAAATGATCAAAGCGTGGTGAATTCATCAGGATCGGAGGACATAAACTCATCAACCGAAAACTCCTCCACAGGCGCTGAATCGGCGTTGTTAGACAACATTTGCAATGACGATTCGTTAATGAACAGCCTGTGGATGGATGAAACACCTCTGATTGATGCATTGTGGAACGACAACAATCAAGTAGAACTTGATCAAGGAGGGATCGATTACAATATCATTGAGAATAATGGAATGGGATTCCAATCTAATTGGGAGGACAATTGTGCATGGCTTTTGGACTGCCAAGACTTTGGTATTCATGATTTTGGTATGGATTGCTTCAATGATGTGGAAGTAAATGGTAACAAAACACTAGAGGTGGGGGGAAAATTAAGCACTAAAATAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

303

Amino Acids

33.87

Weight (kDa)

5.01

Isoelectric Point (pI)

50.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 2.3e-15 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 68 3.1e-07 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 71 - 111 4.7e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 116 2.7e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 491
AciI CCGC 1 cut(s) 184
AclWI GGATC 3 cut(s) 359, 556, 728
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 1 cut(s) 538
AcuI CTGAAG 2 cut(s) 234, 240
AfiI CCNNNNNNNGG 1 cut(s) 652
AgsI TTSAA 2 cut(s) 196, 841
AjnI CCWGG 1 cut(s) 289
AluBI AGCT 5 cut(s) 73, 81, 218, 346, 482
AluI AGCT 5 cut(s) 73, 81, 218, 346, 482
AlwI GGATC 3 cut(s) 359, 556, 728
AlwNI CAGNNNCTG 1 cut(s) 593
AoxI GGCC 4 cut(s) 44, 97, 133, 202
ApeKI GCWGC 1 cut(s) 346
ApoI RAATTY 1 cut(s) 538
Asp700I GAANNNNTTC 1 cut(s) 384
AspLEI GCGC 1 cut(s) 593
AspS9I GGNCC 2 cut(s) 44, 267
AsuHPI GGTGA 2 cut(s) 82, 547
AvaII GGWCC 1 cut(s) 267
BalI TGGCCA 1 cut(s) 99
BbvCI CCTCAGC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 333
BccI CCATC 2 cut(s) 349, 646
BceAI ACGGC 1 cut(s) 391
BciT130I CCWGG 1 cut(s) 291
BclI TGATCA 5 cut(s) 451, 460, 484, 523, 709
BfaI CTAG 1 cut(s) 872
BfoI RGCGCY 1 cut(s) 594
BisI GCNGC 1 cut(s) 347
BlsI GCNGC 1 cut(s) 348
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 1 cut(s) 267
BmgT120I GGNCC 2 cut(s) 44, 267
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 1 cut(s) 291
BmsI GCATC 1 cut(s) 664
BplI GAGNNNNNCTC 2 cut(s) 192, 224
Bpu10I CCTNAGC 2 cut(s) 57, 350
Bsa29I ATCGAT 2 cut(s) 237, 723
BsaBI GATNNNNATC 1 cut(s) 760
BsaJI CCNNGG 2 cut(s) 47, 290
Bsc4I CCNNNNNNNGG 1 cut(s) 652
Bse118I RCCGGY 1 cut(s) 141
Bse1I ACTGG 1 cut(s) 100
Bse3DI GCAATG 1 cut(s) 628
Bse8I GATNNNNATC 1 cut(s) 760
BseBI CCWGG 1 cut(s) 291
BseCI ATCGAT 2 cut(s) 237, 723
BseDI CCNNGG 2 cut(s) 47, 290
BseGI GGATG 3 cut(s) 360, 657, 661
BseJI GATNNNNATC 1 cut(s) 760
BseLI CCNNNNNNNGG 1 cut(s) 652
BseMI GCAATG 1 cut(s) 628
BseMII CTCAG 1 cut(s) 48
BseNI ACTGG 1 cut(s) 100
BseRI GAGGAG 2 cut(s) 74, 571
BseXI GCAGC 1 cut(s) 333
BshFI GGCC 4 cut(s) 46, 99, 135, 204
BshVI ATCGAT 2 cut(s) 237, 723
BsiSI CCGG 1 cut(s) 142
BslI CCNNNNNNNGG 1 cut(s) 652
BsnI GGCC 4 cut(s) 46, 99, 135, 204
Bsp19I CCATGG 1 cut(s) 47
BspACI CCGC 1 cut(s) 184
BspANI GGCC 4 cut(s) 46, 99, 135, 204
BspCNI CTCAG 1 cut(s) 49
BspDI ATCGAT 2 cut(s) 237, 723
BspHI TCATGA 2 cut(s) 454, 817
BspLI GGNNCC 1 cut(s) 164
BspPI GGATC 3 cut(s) 359, 556, 728
BsrDI GCAATG 1 cut(s) 628
BsrFI RCCGGY 1 cut(s) 141
BsrI ACTGG 1 cut(s) 100
BssAI RCCGGY 1 cut(s) 141
BssECI CCNNGG 2 cut(s) 47, 290
BssT1I CCWWGG 1 cut(s) 47
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 2 cut(s) 15, 228
Bst6I CTCTTC 2 cut(s) 191, 393
BstC8I GCNNGC 4 cut(s) 133, 161, 280, 480
BstDEI CTNAG 2 cut(s) 57, 350
BstDSI CCRYGG 1 cut(s) 47
BstF5I GGATG 3 cut(s) 360, 657, 661
BstH2I RGCGCY 1 cut(s) 594
BstHHI GCGC 1 cut(s) 593
BstMWI GCNNNNNNNGC 1 cut(s) 599
BstNI CCWGG 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 289
BstV1I GCAGC 1 cut(s) 333
BstXI CCANNNNNNTGG 1 cut(s) 809
Bsu15I ATCGAT 2 cut(s) 237, 723
BsuRI GGCC 4 cut(s) 46, 99, 135, 204
BsuTUI ATCGAT 2 cut(s) 237, 723
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 3 cut(s) 360, 657, 661
BtsIMutI CAGTG 2 cut(s) 107, 210
Cac8I GCNNGC 4 cut(s) 133, 161, 280, 480
CaiI CAGNNNCTG 1 cut(s) 593
CciI TCATGA 2 cut(s) 454, 817
CfoI GCGC 1 cut(s) 593
Cfr10I RCCGGY 1 cut(s) 141
Cfr13I GGNCC 2 cut(s) 44, 267
ClaI ATCGAT 2 cut(s) 237, 723
CviAII CATG 6 cut(s) 48, 377, 449, 455, 786, 818
DdeI CTNAG 2 cut(s) 57, 350
EaeI YGGCCR 1 cut(s) 97
Eam1104I CTCTTC 2 cut(s) 191, 393
EarI CTCTTC 2 cut(s) 191, 393
EciI GGCGGA 1 cut(s) 173
Eco130I CCWWGG 1 cut(s) 47
Eco147I AGGCCT 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 267
Eco57I CTGAAG 2 cut(s) 234, 240
EcoRI GAATTC 1 cut(s) 538
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 47
EcoT22I ATGCAT 1 cut(s) 679
ErhI CCWWGG 1 cut(s) 47
FaeI CATG 6 cut(s) 51, 380, 452, 458, 789, 821
FalI AAGNNNNNCTT 2 cut(s) 690, 722
FatI CATG 6 cut(s) 47, 376, 448, 454, 785, 817
FbaI TGATCA 5 cut(s) 451, 460, 484, 523, 709
FblI GTMKAC 1 cut(s) 491
Fnu4HI GCNGC 1 cut(s) 347
FokI GGATG 3 cut(s) 367, 664, 668
Fsp4HI GCNGC 1 cut(s) 347
FspBI CTAG 1 cut(s) 872
GlaI GCGC 1 cut(s) 592
GluI GCNGC 1 cut(s) 347
HaeII RGCGCY 1 cut(s) 594
HaeIII GGCC 4 cut(s) 46, 99, 135, 204
HapII CCGG 1 cut(s) 142
HhaI GCGC 1 cut(s) 593
Hin1II CATG 6 cut(s) 51, 380, 452, 458, 789, 821
Hin6I GCGC 1 cut(s) 591
HinP1I GCGC 1 cut(s) 591
HinfI GANTC 4 cut(s) 316, 596, 629, 756
HpaII CCGG 1 cut(s) 142
HphI GGTGA 2 cut(s) 82, 547
Hpy166II GTNNAC 1 cut(s) 492
Hpy188I TCNGA 2 cut(s) 553, 669
Hpy188III TCNNGA 5 cut(s) 313, 337, 455, 546, 818
Hpy8I GTNNAC 1 cut(s) 492
HpyAV CCTTC 3 cut(s) 146, 215, 395
HpyCH4III ACNGT 2 cut(s) 15, 228
HpyCH4V TGCA 5 cut(s) 159, 278, 621, 677, 785
HpyF10VI GCNNNNNNNGC 1 cut(s) 599
HpyF3I CTNAG 2 cut(s) 57, 350
Hsp92II CATG 6 cut(s) 51, 380, 452, 458, 789, 821
HspAI GCGC 1 cut(s) 591
Ksp22I TGATCA 5 cut(s) 451, 460, 484, 523, 709
LmnI GCTCC 1 cut(s) 168
Lsp1109I GCAGC 1 cut(s) 333
LweI GCATC 1 cut(s) 664
MaeI CTAG 1 cut(s) 872
MaeIII GTNAC 3 cut(s) 20, 370, 860
MboII GAAGA 6 cut(s) 208, 233, 407, 410, 435, 494
MfeI CAATTG 1 cut(s) 778
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 9 cut(s) 175, 360, 472, 508, 538, 766, 778, 887, 904
MluNI TGGCCA 1 cut(s) 99
MmeI TCCRAC 2 cut(s) 89, 149
Mox20I TGGCCA 1 cut(s) 99
Mph1103I ATGCAT 1 cut(s) 679
MroXI GAANNNNTTC 1 cut(s) 384
MscI TGGCCA 1 cut(s) 99
MseI TTAA 4 cut(s) 417, 635, 890, 907
MslI CAYNNNNRTG 1 cut(s) 453
Msp20I TGGCCA 1 cut(s) 99
MspA1I CMGCKG 1 cut(s) 482
MspI CCGG 1 cut(s) 142
MspR9I CCNGG 1 cut(s) 291
MunI CAATTG 1 cut(s) 778
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 1 cut(s) 599
NcoI CCATGG 1 cut(s) 47
NlaIII CATG 6 cut(s) 51, 380, 452, 458, 789, 821
NlaIV GGNNCC 1 cut(s) 164
NmuCI GTSAC 2 cut(s) 20, 370
NsiI ATGCAT 1 cut(s) 679
PagI TCATGA 2 cut(s) 454, 817
PceI AGGCCT 1 cut(s) 204
PdmI GAANNNNTTC 1 cut(s) 384
PfeI GAWTC 4 cut(s) 316, 596, 629, 756
PkrI GCNGC 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 164
PspPI GGNCC 2 cut(s) 44, 267
PstNI CAGNNNCTG 1 cut(s) 593
PvuII CAGCTG 1 cut(s) 482
RseI CAYNNNNRTG 1 cut(s) 453
SaqAI TTAA 4 cut(s) 417, 635, 890, 907
SatI GCNGC 1 cut(s) 347
Sau96I GGNCC 2 cut(s) 44, 267
ScrFI CCNGG 1 cut(s) 291
SetI ASST 8 cut(s) 75, 83, 220, 266, 348, 484, 667, 879
SfaNI GCATC 1 cut(s) 664
SgrAI CRCCGGYG 1 cut(s) 141
SinI GGWCC 1 cut(s) 267
SmiMI CAYNNNNRTG 1 cut(s) 453
Sse9I AATT 9 cut(s) 175, 360, 472, 508, 538, 766, 778, 887, 904
SseBI AGGCCT 1 cut(s) 204
SsiI CCGC 1 cut(s) 184
SspI AATATT 1 cut(s) 415
SspMI CTAG 1 cut(s) 872
StuI AGGCCT 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 1 cut(s) 47
TaaI ACNGT 2 cut(s) 15, 228
TaqI TCGA 2 cut(s) 237, 723
TasI AATT 9 cut(s) 175, 360, 472, 508, 538, 766, 778, 887, 904
TfiI GAWTC 4 cut(s) 316, 596, 629, 756
Tru1I TTAA 4 cut(s) 417, 635, 890, 907
Tru9I TTAA 4 cut(s) 417, 635, 890, 907
TscAI CASTG 2 cut(s) 107, 217
TseFI GTSAC 2 cut(s) 20, 370
TseI GCWGC 1 cut(s) 346
Tsp45I GTSAC 2 cut(s) 20, 370
TspDTI ATGAA 5 cut(s) 393, 531, 653, 672, 806
TspGWI ACGGA 1 cut(s) 155
TspRI CASTG 2 cut(s) 107, 217
VpaK11BI GGWCC 1 cut(s) 267
XapI RAATTY 1 cut(s) 538
XcmI CCANNNNNNNNNTGG 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 491
XmnI GAANNNNTTC 1 cut(s) 384
XspI CTAG 1 cut(s) 872
Zsp2I ATGCAT 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.