Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0004268

Physical Location & Seq

Genomic Coordinates Reverse (-)

30713804 .. 30719742

Gene Structure 5939 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1140500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3123 bp

ATGGCTGCGGCAGCAGGAGGAACGGCATTGTCGTTTTCGGTCGCGTCGATGGTGGAGGACGTGCTTCAACAGCACGGCACTCGCCTCGGCGATCTTAATTTGGAGTCTCGAAAAGCTGAGGAAGCCGCATCAAGGAGAAATGAGGCGGCCGGGTGGCTTCGAAAGATGATCGGCGTTGTTGCCGCTAAAGATATGCCGGCAGAGCCTTCGGAGGAAGAGTTTAGGCTTGGATTGAGAAGTGGGATAATCCTCTGCAATGCGATCAATAAAGTTCAACCTGGAGCTGTGCCCAAGGTGGTAGAAAGTCCATGCGATTCAGCTTTGATCCCCGATGGGGCGGCTTTGTCTGCGTTTCAGTACTTCGAGAATGTGAGGAACTTCCTTGTTGCTATCGAGGGGATGGGGCTTCCCACGTTTGAGGCATCTGATTTGGAACAAGGAGGGAAATCTGCAAGAGTTGTAAATACGGTTCTGGTGCTTAAATCTTACAGTGAGGGGAAACAGACCGGGGGAATTGGCATATCGAAATTTGGTGCAAACATAAAACCCACTACTTCTGCCAAGCCGTTTGTAAGAAAAAATTCGGAGCCATTCACGAATTCGCTGTCAAGAACTTCCTCAATGAATGACAAACCCTTGAGTGCTCCGTCCTCTGATCTTAATTCTAATAAGATGCCTAATTCTCACTCCTTGAGTATGCTTGTTCGCGCAGTTTTATTAGATAAGAAGCCTGAAGAAGTTCCGATGTTAGTAGAGTCTGTGCTAAGCAAGGTCATGGAGGAATTTGAACAACGTATGTCCAGCCAATGTGAATTGACGAAACCAAGTTCAAAAGATGTGGCTGTTTCACGTGGAAACAAGTTTCCAACGAAATTCGCTTCTGGTGATAAAAAGGTGGAAGACAAATCACTTGTCAATACAAATCACATCTCGGATGAGGAATCAAAAAGTCGGCTGGTTAAAAAGCAAATGCTCTTTGATCAACAACAAAGAGATGTTCAGGAACTAAAGCAAACTCTTCACACTACCAAATCTGGTATTCAGTTTATGAAAATGAAGTTCCATGAGGAGTTCCACAATATTGGTTTACACATTCACGGCTTAGCTCATGCAGCAAGTGGATATCACAGAGTTCTTGAAGAAAATCGAAAGCTTTACAATCAAGTTCAAGATCTCAAAGGAAGTATCAGGGTTTATTGTCGAGTGAGACCCTTCTTATCTGGAGTATCAAACTGTTTAAGCACTGTGGATCATATAGAAGATGGAAATATCACTATTAACACCCCAGCAAGGCATGGGAACGGACGCAAGTCCTTCAGTTTCAACAAAGTATTCGGGCCATCTGCAACCCAAGCTGATGTTTTCTCTGATATGCAACCACTGATTCGGTCCGTCCTTGATGGTTACAATGTTTGCATATTTGCATACGGGCAAACTGGATCAGGCAAAACTTACACTATGAGTGGACCCAGAGAACTTACAGAGAAAAGCCAAGGTGTAAATTATAGGGCATTGGGAGACTTGTTTTTTATAGCAGATCGAAGAAAGGACACTTTCCGGTATGATGTTTCTGTTCAAATGATTGAGATCTATAACGAGCAAGTACGAGATCTCCTTGTTACTGATGGGAGTCATGAAGGTCAAGCAACATGTCAAAACACTTGTGATAAACTAAAAACTAAAGTTGTATTTTCGTTGTCTTTCACACATTCCAGGCTTTCCTTAACTATTCACACATTAGAAATTCGTAATAGTTCTCAAACAGGTCTCAGTGTGCCAGATGCAAATCTTGTTCCTGTATCATCAACATCTGATGTTATCGATCTGATGAACCTTGGACAAAAGAATCGTGTTGTGGGTGCAACTGCCCTAAATGACCGTAGTAGTCGTTCTCACAGTTGCTTGAATGTTCATGTTCAAGGAAGGGATTTGACATCTGGAGCTATTCTTCGTGGCTGTATGCATCTGGTTGATCTTGCAGGAAGTGAGAGGGTGGACAAATCTGAGGTGACTGGGGATAGATTAAAAGAAGCTCAACATATTAACAAATCACTCTCAGCTTTAGGCGATGTTATTGCTTCCCTTGCCCAAAAGAATCCACATGTTCCTTATAGAAATAGTAAACTAACACAGTTGCTCCAAGATTCACTTGGTGGACAGGCCAAGACACTTATGTTTGTTCACATAAGCCCTGAGCCAGATGCTGTTGGTGAAACAATAAGCACGCTCAAATTTGCAGAGCGAGTAGCCACAGTTGAACTTGGTGCAGCCCGGGTAAACAAAGATAGCACAGATGTCAAAGCTCTCAAAGAACAGATTGCTGATCTTAAGGCACAACTAGCCAGGAAAGAGGAAGCTGAACACAATCAACCCCCAGTCTCTGGCAGCTCTGATAAATACAGGATGAAATCTGGTGAGATGTCACCATTTCATTCTAAACATCAGGGTACAGACATGATGGGGGATCAGAATGGTTGCCGGACACCAATGATCAATGTAGGCAAAATCGAGATTAACGACAATTCTGCTTTGAGGACAAAAAGACAAAGCTTTGATCTTGATGAGCTACTAGGGAATTCATCTCCCTGGCCCCCAGTTGATAGTCCTATTCAGAACTGTGGGGAGGATGATAGAGACATGAGCTCGGGCGAGTGGGTTGATAAGGTTATGGTAAACAAGCTAGAAGCAAGCAGAGCTGGAAATCCTTTAGGATCATGGGGAGCAGACAGTGGGAATTTATCTGATGGTTTCTACCAGAAATATCTCCAGGATTCTTCCAAGGTTTATCCAGAACAATCCTACAATATGTTCGCGGGAAGCAATGGGTTCAATGTTGCTAGCACCGATGACATGGATGATCTTGATGCTGCCACCAGTGACTCTTCAGAGCATGATTTGCTCTGGCAATTCAATCAAACTAAACTTACCAGCATAGGCAATGGGATTGCTTCAAAAACCAAGAAACCTAATGGAAAGCCAATGAAAGGCCTAGAACCAAGCAAGAGCTTTAATCCTTCTCTAGGCCCTTCACCGTCACGAAAACCTTTGAACGGGGTACCACATCGGACTGCAAGGCAGCCAGTTCCGGCTGAGATGAAACGCAAAGCTGGGAGTAGAAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006810 GO:0006928 GO:0007017 GO:0007018 GO:0007097 GO:0008017 GO:0008092 GO:0008150 GO:0008569 GO:0009987 GO:0015629 GO:0015630 GO:0015631 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031534 GO:0032991 GO:0042623 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043621 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0046907 GO:0051012 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051647 GO:0051649 GO:0051656 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CH Kinesin Microtub_bd

Protein Analysis

1041

Amino Acids

113.8

Weight (kDa)

8.45

Isoelectric Point (pI)

44.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CH	PF00307	45 - 163	2.4e-11	Calponin homology (CH) domain
Microtub_bd	PF16796	381 - 539	2.6e-43	Microtubule binding
Kinesin	PF00225	418 - 751	6.1e-105	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004268)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47500 AT2G47500
fragaria_vesca	FvH4_2g26160 FvH4_2g26160 FvH4_2g26160
malus_domestica	MD00G1140500.v1.1 MD03G1292600.v1.1
prunus_persica	Prupe.8G265600_v2.0.a1 Prupe.8G265600_v2.0.a1
pyrus_communis	pycom03g23180 pycom11g27320
rosa_chinensis	RchiOBHm_Chr5g0061681 RchiOBHm_Chr6g0294551 RchiOBHm_Chr6g0294561
rosa_laevigata	RLG00000003184 RLG00000011860
rosa_multiflora	Rmu_co8460257.1_g000001 Rmu_sc0004268.1_g000003 Rmu_sc0004268.1_g000004 Rmu_sc0042489.1_g000001 Rmu_ssc0000248.1_g000004
rosa_roxburghii	Rroxscaffold_3G00250060 Rroxscaffold_7G00173230 Rroxscaffold_7G00197400
rosa_rugosa	Rorug06G0246000
rosa_samantha	Rh6AG358300 Rh6BG365000 Rh6BG365100 Rh6CG372100 Rh6DG358900 Rh7DG248300
rosa_wichuraiana	Rw6G031250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	3055
AccB1I	GGYRCC	1 cut(s)	3055
AccB7I	CCANNNNNTGG	1 cut(s)	2381
AccII	CGCG	3 cut(s)	44, 708, 2813
AciI	CCGC	6 cut(s)	8, 126, 146, 183, 338, 2813
AclWI	GGATC	5 cut(s)	319, 1257, 1447, 2472, 2719
AcoI	YGGCCR	1 cut(s)	147
AcsI	RAATTY	9 cut(s)	527, 580, 598, 782, 872, 1743, 2231, 2575, 2734
AcuI	CTGAAG	3 cut(s)	753, 1300, 2868
AcvI	CACGTG	1 cut(s)	851
AdeI	CACNNNGTG	1 cut(s)	2153
AfaI	GTAC	4 cut(s)	359, 1605, 2449, 3057
AfiI	CCNNNNNNNGG	7 cut(s)	132, 334, 1290, 2381, 2984, 3020, 3050
AflII	CTTAAG	1 cut(s)	2327
AflIII	ACRYGT	2 cut(s)	1649, 2101
AjiI	CACGTC	1 cut(s)	61
AjnI	CCWGG	5 cut(s)	277, 1712, 2342, 2585, 2766
Alw21I	GWGCWC	2 cut(s)	646, 2645
Alw26I	GTCTC	6 cut(s)	111, 1201, 1512, 1772, 2383, 2628
AlwI	GGATC	5 cut(s)	319, 1257, 1447, 2472, 2719
AlwNI	CAGNNNCTG	2 cut(s)	2204, 2381
Ama87I	CYCGRG	2 cut(s)	2271, 2644
AoxI	GGCC	6 cut(s)	147, 1337, 2160, 2588, 2986, 3022
ApeKI	GCWGC	7 cut(s)	5, 11, 1112, 2267, 2385, 2867, 3076
ApoI	RAATTY	9 cut(s)	527, 580, 598, 782, 872, 1743, 2231, 2575, 2734
ArsI	GACNNNNNNTTYG	4 cut(s)	590, 622, 897, 929
Asp700I	GAANNNNTTC	2 cut(s)	580, 738
Asp718I	GGTACC	1 cut(s)	3055
AspLEI	GCGC	1 cut(s)	710
AspS9I	GGNCC	5 cut(s)	1337, 1389, 1466, 2589, 3023
AsuC2I	CCSGG	4 cut(s)	151, 508, 2272, 2273
AsuHPI	GGTGA	6 cut(s)	896, 2020, 2222, 2415, 2426, 3021
AsuII	TTCGAA	1 cut(s)	160
AsuNHI	GCTAGC	1 cut(s)	2837
AvaI	CYCGRG	2 cut(s)	2271, 2644
AvaII	GGWCC	2 cut(s)	1389, 1466
BaeGI	GKGCMC	1 cut(s)	291
BanI	GGYRCC	1 cut(s)	3055
BanII	GRGCYC	1 cut(s)	2645
BbrPI	CACGTG	1 cut(s)	851
BbsI	GAAGAC	1 cut(s)	906
Bbv12I	GWGCWC	2 cut(s)	646, 2645
BbvCI	CCTCAGC	1 cut(s)	117
BbvI	GCAGC	6 cut(s)	23, 1124, 2279, 2397, 2854, 3088
BccI	CCATC	9 cut(s)	43, 326, 394, 1256, 1348, 1394, 1619, 2452, 2738
BceAI	ACGGC	4 cut(s)	39, 91, 550, 1114
BciT130I	CCWGG	5 cut(s)	279, 1714, 2344, 2587, 2768
BclI	TGATCA	2 cut(s)	979, 2490
BcnI	CCSGG	4 cut(s)	151, 508, 2272, 2273
BcoDI	GTCTC	6 cut(s)	111, 1201, 1512, 1772, 2383, 2628
BfaI	CTAG	6 cut(s)	2339, 2570, 2681, 2838, 2990, 3020
BfrI	CTTAAG	1 cut(s)	2327
BglII	AGATCT	3 cut(s)	1171, 1587, 1609
BlpI	GCTNAGC	2 cut(s)	764, 1102
BmcAI	AGTACT	1 cut(s)	359
Bme1390I	CCNGG	9 cut(s)	151, 279, 508, 1714, 2272, 2273, 2344, 2587, 2768
Bme18I	GGWCC	2 cut(s)	1389, 1466
BmeT110I	CYCGRG	2 cut(s)	2271, 2644
BmgBI	CACGTC	1 cut(s)	61
BmgT120I	GGNCC	5 cut(s)	1337, 1389, 1466, 2589, 3023
BmiI	GGNNCC	4 cut(s)	588, 1468, 2591, 3057
BmrFI	CCNGG	9 cut(s)	151, 279, 508, 1714, 2272, 2273, 2344, 2587, 2768
BmrI	ACTGGG	3 cut(s)	2022, 2369, 2588
BmsI	GCATC	7 cut(s)	137, 431, 663, 1771, 1972, 2191, 2854
BmtI	GCTAGC	1 cut(s)	2841
BmuI	ACTGGG	3 cut(s)	2022, 2369, 2588
BoxI	GACNNNNGTC	1 cut(s)	1309
BpiI	GAAGAC	1 cut(s)	906
BpmI	CTGGAG	4 cut(s)	300, 1242, 1959, 2750
Bpu10I	CCTNAGC	2 cut(s)	117, 2193
Bpu1102I	GCTNAGC	2 cut(s)	764, 1102
Bpu14I	TTCGAA	1 cut(s)	160
BpuEI	CTTGAG	2 cut(s)	658, 712
BpuMI	CCSGG	4 cut(s)	151, 508, 2272, 2273
Bsa29I	ATCGAT	1 cut(s)	1821
BsaAI	YACGTR	1 cut(s)	851
BsaBI	GATNNNNATC	2 cut(s)	1586, 1785
BsaI	GGTCTC	2 cut(s)	1201, 1772
BsaJI	CCNNGG	8 cut(s)	85, 291, 507, 1492, 1834, 2271, 2585, 2778
BsaWI	WCCGGW	1 cut(s)	1557
BsaXI	ACNNNNNCTCC	6 cut(s)	203, 233, 1596, 1626, 2121, 2151
Bsc4I	CCNNNNNNNGG	7 cut(s)	132, 334, 1290, 2381, 2984, 3020, 3050
Bse118I	RCCGGY	1 cut(s)	196
Bse1I	ACTGG	6 cut(s)	1441, 2017, 2375, 2594, 2874, 3080
Bse3DI	GCAATG	3 cut(s)	262, 2827, 2944
Bse8I	GATNNNNATC	2 cut(s)	1586, 1785
BseBI	CCWGG	5 cut(s)	279, 1714, 2344, 2587, 2768
BseCI	ATCGAT	1 cut(s)	1821
BseDI	CCNNGG	8 cut(s)	85, 291, 507, 1492, 1834, 2271, 2585, 2778
BseGI	GGATG	5 cut(s)	405, 940, 2409, 2632, 2860
BseJI	GATNNNNATC	2 cut(s)	1586, 1785
BseLI	CCNNNNNNNGG	7 cut(s)	132, 334, 1290, 2381, 2984, 3020, 3050
BseMI	GCAATG	3 cut(s)	262, 2827, 2944
BseMII	CTCAG	6 cut(s)	108, 1783, 1995, 2070, 2184, 3081
BseNI	ACTGG	6 cut(s)	1441, 2017, 2375, 2594, 2874, 3080
BseRI	GAGGAG	1 cut(s)	1082
BseSI	GKGCMC	1 cut(s)	291
BseX3I	CGGCCG	1 cut(s)	147
BseXI	GCAGC	6 cut(s)	23, 1124, 2279, 2397, 2854, 3088
BseYI	CCCAGC	2 cut(s)	1285, 3107
BsgI	GTGCAG	1 cut(s)	2286
Bsh1236I	CGCG	3 cut(s)	44, 708, 2813
Bsh1285I	CGRYCG	2 cut(s)	42, 150
BshFI	GGCC	6 cut(s)	149, 1339, 2162, 2590, 2988, 3024
BshNI	GGYRCC	1 cut(s)	3055
BshVI	ATCGAT	1 cut(s)	1821
BsiEI	CGRYCG	2 cut(s)	42, 150
BsiHKAI	GWGCWC	2 cut(s)	646, 2645
BsiHKCI	CYCGRG	2 cut(s)	2271, 2644
BsiSI	CCGG	7 cut(s)	150, 197, 507, 1558, 2272, 2479, 3086
BslI	CCNNNNNNNGG	7 cut(s)	132, 334, 1290, 2381, 2984, 3020, 3050
BsmAI	GTCTC	6 cut(s)	111, 1201, 1512, 1772, 2383, 2628
BsnI	GGCC	6 cut(s)	149, 1339, 2162, 2590, 2988, 3024
Bso31I	GGTCTC	2 cut(s)	1201, 1772
BsoBI	CYCGRG	2 cut(s)	2271, 2644
Bsp119I	TTCGAA	1 cut(s)	160
Bsp1286I	GDGCHC	3 cut(s)	291, 646, 2645
Bsp1720I	GCTNAGC	2 cut(s)	764, 1102
BspACI	CCGC	6 cut(s)	8, 126, 146, 183, 338, 2813
BspANI	GGCC	6 cut(s)	149, 1339, 2162, 2590, 2988, 3024
BspCNI	CTCAG	6 cut(s)	109, 1782, 1996, 2069, 2185, 3082
BspDI	ATCGAT	1 cut(s)	1821
BspFNI	CGCG	3 cut(s)	44, 708, 2813
BspHI	TCATGA	1 cut(s)	1633
BspLI	GGNNCC	4 cut(s)	588, 1468, 2591, 3057
BspOI	GCTAGC	1 cut(s)	2841
BspPI	GGATC	5 cut(s)	319, 1257, 1447, 2472, 2719
BspT104I	TTCGAA	1 cut(s)	160
BspT107I	GGYRCC	1 cut(s)	3055
BspTI	CTTAAG	1 cut(s)	2327
BspTNI	GGTCTC	2 cut(s)	1201, 1772
BsrDI	GCAATG	3 cut(s)	262, 2827, 2944
BsrFI	RCCGGY	1 cut(s)	196
BsrI	ACTGG	6 cut(s)	1441, 2017, 2375, 2594, 2874, 3080
BssAI	RCCGGY	1 cut(s)	196
BssECI	CCNNGG	8 cut(s)	85, 291, 507, 1492, 1834, 2271, 2585, 2778
BssT1I	CCWWGG	4 cut(s)	291, 1492, 1834, 2778
Bst2UI	CCWGG	5 cut(s)	279, 1714, 2344, 2587, 2768
Bst6I	CTCTTC	3 cut(s)	210, 1023, 2887
BstAFI	CTTAAG	1 cut(s)	2327
BstAPI	GCANNNNNTGC	1 cut(s)	2896
BstBAI	YACGTR	1 cut(s)	851
BstBI	TTCGAA	1 cut(s)	160
BstC8I	GCNNGC	4 cut(s)	198, 2225, 2689, 2839
BstDEI	CTNAG	8 cut(s)	117, 764, 1102, 1769, 2004, 2056, 2193, 3090
BstENI	CCTNNNNNAGG	1 cut(s)	3018
BstF5I	GGATG	5 cut(s)	405, 940, 2409, 2632, 2860
BstFNI	CGCG	3 cut(s)	44, 708, 2813
BstHHI	GCGC	1 cut(s)	710
BstMAI	GTCTC	6 cut(s)	111, 1201, 1512, 1772, 2383, 2628
BstMCI	CGRYCG	2 cut(s)	42, 150
BstNI	CCWGG	5 cut(s)	279, 1714, 2344, 2587, 2768
BstNSI	RCATGY	2 cut(s)	1653, 2105
BstPAI	GACNNNNGTC	1 cut(s)	1309
BstSCI	CCNGG	9 cut(s)	149, 277, 506, 1712, 2270, 2271, 2342, 2585, 2766
BstSLI	GKGCMC	1 cut(s)	291
BstUI	CGCG	3 cut(s)	44, 708, 2813
BstV1I	GCAGC	6 cut(s)	23, 1124, 2279, 2397, 2854, 3088
BstV2I	GAAGAC	1 cut(s)	906
BstX2I	RGATCY	3 cut(s)	1171, 1587, 1609
BstXI	CCANNNNNNTGG	1 cut(s)	1082
BstYI	RGATCY	3 cut(s)	1171, 1587, 1609
BstZI	CGGCCG	1 cut(s)	147
Bsu15I	ATCGAT	1 cut(s)	1821
BsuRI	GGCC	6 cut(s)	149, 1339, 2162, 2590, 2988, 3024
BsuTUI	ATCGAT	1 cut(s)	1821
BtgZI	GCGATG	1 cut(s)	2082
BtrI	CACGTC	1 cut(s)	61
BtsCI	GGATG	5 cut(s)	405, 940, 2409, 2632, 2860
BtsIMutI	CAGTG	6 cut(s)	496, 1242, 1379, 1777, 2734, 2881
Cac8I	GCNNGC	4 cut(s)	198, 2225, 2689, 2839
CaiI	CAGNNNCTG	2 cut(s)	2204, 2381
CciI	TCATGA	1 cut(s)	1633
CfoI	GCGC	1 cut(s)	710
Cfr10I	RCCGGY	1 cut(s)	196
Cfr13I	GGNCC	5 cut(s)	1337, 1389, 1466, 2589, 3023
Cfr9I	CCCGGG	1 cut(s)	2271
ClaI	ATCGAT	1 cut(s)	1821
CpoI	CGGWCCG	1 cut(s)	1389
CseI	GACGC	2 cut(s)	33, 1314
Csp6I	GTAC	4 cut(s)	358, 1604, 2448, 3056
CspCI	CAANNNNNGTGG	2 cut(s)	819, 854
CspI	CGGWCCG	1 cut(s)	1389
CviQI	GTAC	4 cut(s)	358, 1604, 2448, 3056
DdeI	CTNAG	8 cut(s)	117, 764, 1102, 1769, 2004, 2056, 2193, 3090
DraIII	CACNNNGTG	1 cut(s)	2153
EaeI	YGGCCR	1 cut(s)	147
EagI	CGGCCG	1 cut(s)	147
Eam1104I	CTCTTC	3 cut(s)	210, 1023, 2887
EarI	CTCTTC	3 cut(s)	210, 1023, 2887
Ecl136II	GAGCTC	1 cut(s)	2643
EclXI	CGGCCG	1 cut(s)	147
Eco130I	CCWWGG	4 cut(s)	291, 1492, 1834, 2778
Eco147I	AGGCCT	1 cut(s)	2988
Eco24I	GRGCYC	1 cut(s)	2645
Eco31I	GGTCTC	2 cut(s)	1201, 1772
Eco32I	GATATC	1 cut(s)	1124
Eco47I	GGWCC	2 cut(s)	1389, 1466
Eco52I	CGGCCG	1 cut(s)	147
Eco53kI	GAGCTC	1 cut(s)	2643
Eco57I	CTGAAG	3 cut(s)	753, 1300, 2868
Eco72I	CACGTG	1 cut(s)	851
Eco88I	CYCGRG	2 cut(s)	2271, 2644
EcoICRI	GAGCTC	1 cut(s)	2643
EcoNI	CCTNNNNNAGG	1 cut(s)	3018
EcoO109I	RGGNCCY	1 cut(s)	3023
EcoRI	GAATTC	2 cut(s)	598, 2575
EcoRII	CCWGG	5 cut(s)	277, 1712, 2342, 2585, 2766
EcoRV	GATATC	1 cut(s)	1124
EcoT14I	CCWWGG	4 cut(s)	291, 1492, 1834, 2778
EcoT22I	ATGCAT	1 cut(s)	1965
EcoT38I	GRGCYC	1 cut(s)	2645
ErhI	CCWWGG	4 cut(s)	291, 1492, 1834, 2778
FalI	AAGNNNNNCTT	2 cut(s)	2133, 2165
FauI	CCCGC	1 cut(s)	2806
FbaI	TGATCA	2 cut(s)	979, 2490
FokI	GGATG	5 cut(s)	412, 947, 2416, 2639, 2867
FriOI	GRGCYC	1 cut(s)	2645
FspBI	CTAG	6 cut(s)	2339, 2570, 2681, 2838, 2990, 3020
GlaI	GCGC	1 cut(s)	709
GsaI	CCCAGC	2 cut(s)	1289, 3111
GsuI	CTGGAG	4 cut(s)	300, 1242, 1959, 2750
HaeIII	GGCC	6 cut(s)	149, 1339, 2162, 2590, 2988, 3024
HapII	CCGG	7 cut(s)	150, 197, 507, 1558, 2272, 2479, 3086
HgaI	GACGC	2 cut(s)	33, 1314
HhaI	GCGC	1 cut(s)	710
Hin6I	GCGC	1 cut(s)	708
HinP1I	GCGC	1 cut(s)	708
HindIII	AAGCTT	2 cut(s)	1151, 2548
HpaII	CCGG	7 cut(s)	150, 197, 507, 1558, 2272, 2479, 3086
HphI	GGTGA	6 cut(s)	896, 2020, 2222, 2415, 2426, 3021
Hpy166II	GTNNAC	8 cut(s)	1088, 1466, 1996, 2123, 2156, 2182, 2278, 2674
Hpy8I	GTNNAC	8 cut(s)	1088, 1466, 1996, 2123, 2156, 2182, 2278, 2674
Hpy99I	CGWCG	1 cut(s)	49
HpyAV	CCTTC	7 cut(s)	216, 1222, 1324, 1631, 1917, 3024, 3036
HpyCH4IV	ACGT	4 cut(s)	60, 413, 793, 850
HpyF3I	CTNAG	8 cut(s)	117, 764, 1102, 1769, 2004, 2056, 2193, 3090
HpySE526I	ACGT	4 cut(s)	60, 413, 793, 850
HspAI	GCGC	1 cut(s)	708
KpnI	GGTACC	1 cut(s)	3059
KroI	GCCGGC	1 cut(s)	196
KroNI	GCCGGC	1 cut(s)	198
Ksp22I	TGATCA	2 cut(s)	979, 2490
LmnI	GCTCC	6 cut(s)	281, 586, 649, 1940, 2142, 2720
Lsp1109I	GCAGC	6 cut(s)	23, 1124, 2279, 2397, 2854, 3088
LweI	GCATC	7 cut(s)	137, 431, 663, 1771, 1972, 2191, 2854
MaeI	CTAG	6 cut(s)	2339, 2570, 2681, 2838, 2990, 3020
MaeII	ACGT	4 cut(s)	60, 413, 793, 850
MaeIII	GTNAC	6 cut(s)	1403, 1618, 2008, 2421, 2876, 3033
MflI	RGATCY	3 cut(s)	1171, 1587, 1609
MhlI	GDGCHC	3 cut(s)	291, 646, 2645
MlyI	GAGTC	4 cut(s)	113, 764, 1639, 2873
MmeI	TCCRAC	1 cut(s)	890
Mph1103I	ATGCAT	1 cut(s)	1965
MroNI	GCCGGC	1 cut(s)	196
MroXI	GAANNNNTTC	2 cut(s)	580, 738
MspCI	CTTAAG	1 cut(s)	2327
MspI	CCGG	7 cut(s)	150, 197, 507, 1558, 2272, 2479, 3086
MspR9I	CCNGG	9 cut(s)	151, 279, 508, 1714, 2272, 2273, 2344, 2587, 2768
MvaI	CCWGG	5 cut(s)	279, 1714, 2344, 2587, 2768
MvnI	CGCG	3 cut(s)	44, 708, 2813
NaeI	GCCGGC	1 cut(s)	198
NciI	CCSGG	4 cut(s)	151, 508, 2272, 2273
NgoMIV	GCCGGC	1 cut(s)	196
NheI	GCTAGC	1 cut(s)	2837
NlaIV	GGNNCC	4 cut(s)	588, 1468, 2591, 3057
NmeAIII	GCCGAG	1 cut(s)	66
NmuCI	GTSAC	4 cut(s)	2008, 2421, 2876, 3033
NsiI	ATGCAT	1 cut(s)	1965
NspI	RCATGY	2 cut(s)	1653, 2105
NspV	TTCGAA	1 cut(s)	160
PagI	TCATGA	1 cut(s)	1633
PceI	AGGCCT	1 cut(s)	2988
PciI	ACATGT	2 cut(s)	1649, 2101
PcsI	WCGNNNNNNNCGW	3 cut(s)	29, 44, 2514
PdiI	GCCGGC	1 cut(s)	198
PdmI	GAANNNNTTC	2 cut(s)	580, 738
PfeI	GAWTC	7 cut(s)	314, 941, 1384, 1846, 2095, 2144, 2771
PflMI	CCANNNNNTGG	1 cut(s)	2381
PfoI	TCCNGGA	1 cut(s)	2766
PleI	GAGTC	4 cut(s)	112, 763, 1638, 2873
PmaCI	CACGTG	1 cut(s)	851
PmlI	CACGTG	1 cut(s)	851
PpsI	GAGTC	4 cut(s)	112, 763, 1638, 2873
Ppu21I	YACGTR	1 cut(s)	851
PscI	ACATGT	2 cut(s)	1649, 2101
PshAI	GACNNNNGTC	1 cut(s)	1309
Psp124BI	GAGCTC	1 cut(s)	2645
Psp6I	CCWGG	5 cut(s)	277, 1712, 2342, 2585, 2766
PspCI	CACGTG	1 cut(s)	851
PspFI	CCCAGC	2 cut(s)	1285, 3107
PspGI	CCWGG	5 cut(s)	277, 1712, 2342, 2585, 2766
PspN4I	GGNNCC	4 cut(s)	588, 1468, 2591, 3057
PspPI	GGNCC	5 cut(s)	1337, 1389, 1466, 2589, 3023
PsrI	GAACNNNNNNTAC	4 cut(s)	453, 485, 1873, 1905
PstNI	CAGNNNCTG	2 cut(s)	2204, 2381
PsuI	RGATCY	3 cut(s)	1171, 1587, 1609
RsaI	GTAC	4 cut(s)	359, 1605, 2449, 3057
RsaNI	GTAC	4 cut(s)	358, 1604, 2448, 3056
Rsr2I	CGGWCCG	1 cut(s)	1389
RsrII	CGGWCCG	1 cut(s)	1389
SacI	GAGCTC	1 cut(s)	2645
Sau96I	GGNCC	5 cut(s)	1337, 1389, 1466, 2589, 3023
ScaI	AGTACT	1 cut(s)	359
SchI	GAGTC	4 cut(s)	113, 764, 1639, 2873
ScrFI	CCNGG	9 cut(s)	151, 279, 508, 1714, 2272, 2273, 2344, 2587, 2768
SduI	GDGCHC	3 cut(s)	291, 646, 2645
SfaNI	GCATC	7 cut(s)	137, 431, 663, 1771, 1972, 2191, 2854
SfuI	TTCGAA	1 cut(s)	160
SinI	GGWCC	2 cut(s)	1389, 1466
SmaI	CCCGGG	1 cut(s)	2273
SmlI	CTYRAG	3 cut(s)	637, 691, 2327
SmoI	CTYRAG	3 cut(s)	637, 691, 2327
SseBI	AGGCCT	1 cut(s)	2988
SsiI	CCGC	6 cut(s)	8, 126, 146, 183, 338, 2813
SspI	AATATT	1 cut(s)	1081
SspMI	CTAG	6 cut(s)	2339, 2570, 2681, 2838, 2990, 3020
SstI	GAGCTC	1 cut(s)	2645
StuI	AGGCCT	1 cut(s)	2988
StyD4I	CCNGG	9 cut(s)	149, 277, 506, 1712, 2270, 2271, 2342, 2585, 2766
StyI	CCWWGG	4 cut(s)	291, 1492, 1834, 2778
TaiI	ACGT	4 cut(s)	63, 416, 796, 853
TaqII	GACCGA	2 cut(s)	28, 1377
TatI	WGTACW	1 cut(s)	357
TauI	GCSGC	5 cut(s)	11, 128, 149, 185, 341
TfiI	GAWTC	7 cut(s)	314, 941, 1384, 1846, 2095, 2144, 2771
TscAI	CASTG	6 cut(s)	496, 1249, 1386, 1777, 2734, 2881
TseFI	GTSAC	4 cut(s)	2008, 2421, 2876, 3033
TseI	GCWGC	7 cut(s)	5, 11, 1112, 2267, 2385, 2867, 3076
Tsp45I	GTSAC	4 cut(s)	2008, 2421, 2876, 3033
TspGWI	ACGGA	3 cut(s)	636, 1317, 1381
TspMI	CCCGGG	1 cut(s)	2271
TspRI	CASTG	6 cut(s)	496, 1249, 1386, 1777, 2734, 2881
Van91I	CCANNNNNTGG	1 cut(s)	2381
Vha464I	CTTAAG	1 cut(s)	2327
VpaK11BI	GGWCC	2 cut(s)	1389, 1466
XagI	CCTNNNNNAGG	1 cut(s)	3018
XapI	RAATTY	9 cut(s)	527, 580, 598, 782, 872, 1743, 2231, 2575, 2734
XceI	RCATGY	2 cut(s)	1653, 2105
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2147
XmaI	CCCGGG	1 cut(s)	2271
XmnI	GAANNNNTTC	2 cut(s)	580, 738
XspI	CTAG	6 cut(s)	2339, 2570, 2681, 2838, 2990, 3020
ZrmI	AGTACT	1 cut(s)	359
Zsp2I	ATGCAT	1 cut(s)	1965

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.