Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0004268

Physical Location & Seq

Genomic Coordinates Reverse (-)

23095237 .. 23100224

Gene Structure 4988 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom03g23180.14

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2589 bp

ATGCCGGCAGAGCCTTCGGAGGAAGAGTTCAGGCTTGCATTGAGAAGTGGGATTATCCTCTGCAATGCTATCAATAAAGTTCAACCTGGAGTTGTGCCCAAGGGAGGGAAATCTGCAAGGGTTGTGAATACGGTTCTGGCGCTTAAATCTTACAGTGAGGGGAAACAGACCGGGGGAAATGGGCCAAATGGCGTATCGAAATTTGGTGCAAACATAAAACCTACTACTTCTACCAAGCCTTTTGTAAGAAAAAATTCGGAGCCATTCACGAACTCGCTGTCAAGGACTTCCTCAATGAATGACAAATCCTTGAGTGCTCTGTCCTCTGACCTTAATTCTAAAAAGATGGTTGGTAACCTAACCTTATATTATCAATTTTGTTTTGTGTATTGTTGCATTCTTGAGTATTCCGATGGGTTATCTCTTTGTTTCTTTTTCATACACTCACGTACTTACAAATTAATCTTACAGACAAAACCAAGTCAAAAAGATGTGACTGTTTCACACGGAAACAAATTTCCGATGAAATTTGCTTCTGGTGATAAAAAGGGTAGCATACTTATGCCGTATATCAAATTTGCAGGAACTAAAGCAAACTCTTCACACTACCAAATCTGGTATTCAGTTTATGAAAATGAAGTTCCACGAGGAGTTCGACAATATTGCTTTATACTCATTCAACTTTCTGCTTCCATAGGTTTGCACATTCATGGCCTAGCTCAAGCAGCGAGTGGATATCACAGAGTTCTTGAAGAAAATCGAAAGCTTTACAATCAAGTCCAAGACCTCAAAGGTAATAGAATAATTGGTGGCTGCCTAATCTCTAAGTTTTGTGTCTACCTTCATAATGGTTTATTGTCGAGTGAGACCCTTCTTATCTGGAGTATCAAACTGCTTAAGTACTGTGGATCGATCGATATAGAAGATGGAAATATCACTATTAACACCCCAGCAAGGCATGGGAACGGACGCAAGTCCTTCAGTTTCAACAAAGTATTCGGGCCATCTGCAACCCAAGCTGATGTTTTCTCTGACATGCACCCATTGATTCGGTCGGTCCTTGATGGTTACAATGTTTGCATATTTGCATACGGCCAAACTGGATCGGGCAAAACTTACACTATGAGTGGACCCAGAGAGCTTACGGAGAAAAGCCAAGGTGTAAATTTTAGGGCATTGGGAGATTTGTTTTTTATAGCAGATCAAAGAAAGGACATTTTCCGTTATGATGTTTCTGTTCAAATGATTGAGATCTATAACGAGCAAGTACGAGATCTCCTTGTTACTGACGGGAGTAACAAAAGATATCCTTTACTTGTTTACTCTACTTGTCAAACACTGGTGAGACTCCAAAAACTTATGTTGTCTTTCACATATTCCAGGCTTTCCTTAACTATTCATACATTAGAAATTCGTAATAGTTCTCAGACAGGTCTCAATGTGCCAGATGCAAATCTTGTTCCTGTCTCATCAACATCTGATGTTATTGATCTGATGAACCTTGGACATAGGAATCGTGTTGTGGGTGCAACTGCCCTGAATGACCGTAGTAGTCGTTCTCACAGTTGCTTGACTGTTCATGTTCAAGGAAGGGATTTGACATCTGGAGCTATTCTTCGTGGCTGTATGCATCTGGTTGATCTTGCGGGAAGTGAGAGGGTGAACAAATCTGAGGTGACCGGAGATAGACTAAAAGAAGCTCAACATATTAACAAATCCCTCTCAGCTTTAGGAGATGTGATCGCTTCCCTTGCCCAAAAGAATCCACATGTTCCATATAGAAATAGTAAACTAACACAGCTGCTCCAAGATTCACTTGGTGGACAGGCGAAGACACTTATGTTTGTTCACATAAGCCCTGAGCCAGATGCTGTTGGTGAAACAATAAGCACGCTCAAATTTGCAGAGCGAGTAGCCACAGTTGAACTTGGTGCAGCCCAAGTAAACAAAGATAGCACAGATGTCAAAGCTCTCAAAGAACAGATTGCTGGTCTTAGGGCATCACTAGCCAGGAAGGAGGAGGAAGCTGAACGCAATCAACGCCAAGTCTGTGGTAGCTCTGATAAATCCAGGATGAAAGCTAGTGAGCTGTCACCATTTCATTCTAACCGTCAGGGTACAGACATGATGGGCGATCTGAATGGTTGCGGGACACCAATGGTAGGCAAAATTGAGGGAATTCATCTCCCTGGCCCCCAGTTGATAGTCCTATCCAGAACTGATCCTGGGGAGCAGACAATGGGAATTTATCTGATGGTTTCTACCAGAAATATCTCCAGGATTCTTCGAAGGTTTATCCAGACCAATCCTACAATATGTTCGTGGAAAGCAACAGGTTCAAGGTTGCTAGCACCGATGAAATGGATGATCTTGATGCTGCCACGAGCGACTCTTCAGAGCCAGATTTGCTCTGGCAATTCAATCAGACTAAACTTACTAGGTAATTCGAACTTGGCATTCTGTTTGAAAAGTTTTAATCCTTCTCTAGGCCCTTCATTGTCACGAAAACCTTTGAACGGGGTACCACATCGGACCGCAAGGCAGCCAGTTCCAGCTGAGATGAAACGAAAAGCTGGGAGTAGAAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006810 GO:0006928 GO:0007017 GO:0007018 GO:0007097 GO:0008017 GO:0008092 GO:0008150 GO:0008569 GO:0009987 GO:0015629 GO:0015630 GO:0015631 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031534 GO:0032991 GO:0042623 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043621 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0046907 GO:0051012 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051647 GO:0051649 GO:0051656 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CH Kinesin Microtub_bd

Protein Analysis

863

Amino Acids

95.02

Weight (kDa)

9.62

Isoelectric Point (pI)

40.65

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004268)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47500 AT2G47500
fragaria_vesca	FvH4_2g26160 FvH4_2g26160 FvH4_2g26160
malus_domestica	MD00G1140500.v1.1 MD03G1292600.v1.1
prunus_persica	Prupe.8G265600_v2.0.a1 Prupe.8G265600_v2.0.a1
pyrus_communis	pycom03g23180 pycom11g27320
rosa_chinensis	RchiOBHm_Chr5g0061681 RchiOBHm_Chr6g0294551 RchiOBHm_Chr6g0294561
rosa_laevigata	RLG00000003184 RLG00000011860
rosa_multiflora	Rmu_co8460257.1_g000001 Rmu_sc0004268.1_g000003 Rmu_sc0004268.1_g000004 Rmu_sc0042489.1_g000001 Rmu_ssc0000248.1_g000004
rosa_roxburghii	Rroxscaffold_3G00250060 Rroxscaffold_7G00173230 Rroxscaffold_7G00197400
rosa_rugosa	Rorug06G0246000
rosa_samantha	Rh6AG358300 Rh6BG365000 Rh6BG365100 Rh6CG372100 Rh6DG358900 Rh7DG248300
rosa_wichuraiana	Rw6G031250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	2521
AccB1I	GGYRCC	1 cut(s)	2521
AccI	GTMKAC	1 cut(s)	837
AciI	CCGC	3 cut(s)	1646, 2148, 2535
AclWI	GGATC	3 cut(s)	916, 1111, 2216
AcoI	YGGCCR	1 cut(s)	1093
AcuI	CTGAAG	2 cut(s)	964, 2378
AdeI	CACNNNGTG	1 cut(s)	1820
AfaI	GTAC	5 cut(s)	451, 902, 1269, 2119, 2523
AfiI	CCNNNNNNNGG	6 cut(s)	104, 105, 954, 2162, 2486, 2516
AflII	CTTAAG	1 cut(s)	896
AflIII	ACRYGT	1 cut(s)	1768
AjnI	CCWGG	7 cut(s)	85, 1379, 2009, 2069, 2188, 2224, 2276
AloI	GAACNNNNNNTCC	2 cut(s)	11, 43
Alw21I	GWGCWC	1 cut(s)	319
Alw26I	GTCTC	4 cut(s)	860, 1339, 1439, 1471
AlwI	GGATC	3 cut(s)	916, 1111, 2216
AlwNI	CAGNNNCTG	1 cut(s)	1871
AoxI	GGCC	6 cut(s)	182, 712, 1001, 1093, 2191, 2488
ApeKI	GCWGC	6 cut(s)	725, 813, 1801, 1934, 2377, 2542
ArsI	GACNNNNNNTTYG	2 cut(s)	263, 295
AseI	ATTAAT	1 cut(s)	461
Asp700I	GAANNNNTTC	1 cut(s)	253
Asp718I	GGTACC	1 cut(s)	2521
AspLEI	GCGC	1 cut(s)	142
AspS9I	GGNCC	7 cut(s)	182, 1001, 1057, 1130, 2192, 2489, 2532
AsuC2I	CCSGG	1 cut(s)	172
AsuHPI	GGTGA	6 cut(s)	551, 1354, 1672, 1687, 1889, 2085
AsuII	TTCGAA	2 cut(s)	2287, 2447
AsuNHI	GCTAGC	1 cut(s)	2347
AvaII	GGWCC	3 cut(s)	1057, 1130, 2532
BaeGI	GKGCMC	1 cut(s)	99
BanI	GGYRCC	1 cut(s)	2521
BauI	CACGAG	2 cut(s)	645, 2382
BbsI	GAAGAC	1 cut(s)	1838
Bbv12I	GWGCWC	1 cut(s)	319
BbvI	GCAGC	6 cut(s)	737, 800, 1788, 1946, 2364, 2554
BccI	CCATC	7 cut(s)	340, 407, 920, 1012, 1058, 2122, 2248
BceAI	ACGGC	2 cut(s)	550, 1108
BcgI	CGANNNNNNTGC	3 cut(s)	31, 645, 679
BciT130I	CCWGG	7 cut(s)	87, 1381, 2011, 2071, 2190, 2226, 2278
BcnI	CCSGG	1 cut(s)	172
BcoDI	GTCTC	4 cut(s)	860, 1339, 1439, 1471
BfaI	CTAG	6 cut(s)	716, 2006, 2082, 2348, 2438, 2486
BfoI	RGCGCY	1 cut(s)	143
BfrI	CTTAAG	1 cut(s)	896
BglII	AGATCT	2 cut(s)	1251, 1273
BisI	GCNGC	6 cut(s)	726, 814, 1802, 1935, 2378, 2543
BlsI	GCNGC	6 cut(s)	727, 815, 1803, 1936, 2379, 2544
BmcAI	AGTACT	1 cut(s)	902
Bme1390I	CCNGG	8 cut(s)	87, 172, 1381, 2011, 2071, 2190, 2226, 2278
Bme18I	GGWCC	3 cut(s)	1057, 1130, 2532
BmgT120I	GGNCC	7 cut(s)	182, 1001, 1057, 1130, 2192, 2489, 2532
BmiI	GGNNCC	4 cut(s)	261, 1132, 2194, 2523
BmrFI	CCNGG	8 cut(s)	87, 172, 1381, 2011, 2071, 2190, 2226, 2278
BmrI	ACTGGG	1 cut(s)	2191
BmsI	GCATC	5 cut(s)	1438, 1639, 1858, 2009, 2364
BmtI	GCTAGC	1 cut(s)	2351
BmuI	ACTGGG	1 cut(s)	2191
BoxI	GACNNNNGTC	1 cut(s)	973
BpiI	GAAGAC	1 cut(s)	1838
BpmI	CTGGAG	4 cut(s)	108, 901, 1626, 2260
Bpu10I	CCTNAGC	1 cut(s)	1860
Bpu14I	TTCGAA	2 cut(s)	2287, 2447
BpuEI	CTTGAG	3 cut(s)	331, 422, 705
BpuMI	CCSGG	1 cut(s)	172
Bsa29I	ATCGAT	2 cut(s)	911, 915
BsaAI	YACGTR	1 cut(s)	449
BsaBI	GATNNNNATC	2 cut(s)	1250, 1452
BsaI	GGTCTC	2 cut(s)	860, 1439
BsaJI	CCNNGG	6 cut(s)	99, 171, 1156, 1501, 2188, 2225
BsaWI	WCCGGW	1 cut(s)	1679
BsaXI	ACNNNNNCTCC	6 cut(s)	11, 41, 1260, 1290, 1788, 1818
Bsc4I	CCNNNNNNNGG	6 cut(s)	104, 105, 954, 2162, 2486, 2516
Bse118I	RCCGGY	1 cut(s)	4
Bse1I	ACTGG	4 cut(s)	1105, 1344, 2197, 2546
Bse3DI	GCAATG	1 cut(s)	70
Bse8I	GATNNNNATC	2 cut(s)	1250, 1452
BseBI	CCWGG	7 cut(s)	87, 1381, 2011, 2071, 2190, 2226, 2278
BseCI	ATCGAT	2 cut(s)	911, 915
BseDI	CCNNGG	6 cut(s)	99, 171, 1156, 1501, 2188, 2225
BseGI	GGATG	2 cut(s)	2079, 2370
BseJI	GATNNNNATC	2 cut(s)	1250, 1452
BseLI	CCNNNNNNNGG	6 cut(s)	104, 105, 954, 2162, 2486, 2516
BseMI	GCAATG	1 cut(s)	70
BseMII	CTCAG	5 cut(s)	1439, 1662, 1737, 1851, 2547
BseNI	ACTGG	4 cut(s)	1105, 1344, 2197, 2546
BseRI	GAGGAG	2 cut(s)	663, 2033
BseSI	GKGCMC	1 cut(s)	99
BseXI	GCAGC	6 cut(s)	737, 800, 1788, 1946, 2364, 2554
BseYI	CCCAGC	2 cut(s)	949, 2573
BsgI	GTGCAG	1 cut(s)	1953
Bsh1285I	CGRYCG	2 cut(s)	915, 1055
BshFI	GGCC	6 cut(s)	184, 714, 1003, 1095, 2193, 2490
BshNI	GGYRCC	1 cut(s)	2521
BshVI	ATCGAT	2 cut(s)	911, 915
BsiEI	CGRYCG	2 cut(s)	915, 1055
BsiHKAI	GWGCWC	1 cut(s)	319
BsiSI	CCGG	3 cut(s)	5, 171, 1680
BslFI	GGGAC	1 cut(s)	2164
BslI	CCNNNNNNNGG	6 cut(s)	104, 105, 954, 2162, 2486, 2516
BsmAI	GTCTC	4 cut(s)	860, 1339, 1439, 1471
BsmFI	GGGAC	1 cut(s)	2164
BsmI	GAATGC	2 cut(s)	396, 2456
BsnI	GGCC	6 cut(s)	184, 714, 1003, 1095, 2193, 2490
Bso31I	GGTCTC	2 cut(s)	860, 1439
Bsp119I	TTCGAA	2 cut(s)	2287, 2447
Bsp1286I	GDGCHC	2 cut(s)	99, 319
BspACI	CCGC	3 cut(s)	1646, 2148, 2535
BspANI	GGCC	6 cut(s)	184, 714, 1003, 1095, 2193, 2490
BspCNI	CTCAG	5 cut(s)	1438, 1663, 1736, 1852, 2548
BspDI	ATCGAT	2 cut(s)	911, 915
BspLI	GGNNCC	4 cut(s)	261, 1132, 2194, 2523
BspOI	GCTAGC	1 cut(s)	2351
BspPI	GGATC	3 cut(s)	916, 1111, 2216
BspT104I	TTCGAA	2 cut(s)	2287, 2447
BspT107I	GGYRCC	1 cut(s)	2521
BspTI	CTTAAG	1 cut(s)	896
BspTNI	GGTCTC	2 cut(s)	860, 1439
BsrDI	GCAATG	1 cut(s)	70
BsrFI	RCCGGY	1 cut(s)	4
BsrI	ACTGG	4 cut(s)	1105, 1344, 2197, 2546
BssAI	RCCGGY	1 cut(s)	4
BssECI	CCNNGG	6 cut(s)	99, 171, 1156, 1501, 2188, 2225
BssSI	CACGAG	2 cut(s)	645, 2382
BssT1I	CCWWGG	3 cut(s)	99, 1156, 1501
Bst2BI	CACGAG	2 cut(s)	645, 2382
Bst2UI	CCWGG	7 cut(s)	87, 1381, 2011, 2071, 2190, 2226, 2278
Bst4CI	ACNGT	9 cut(s)	133, 155, 499, 905, 1547, 1565, 1576, 1921, 2111
Bst6I	CTCTTC	3 cut(s)	18, 604, 2397
BstAFI	CTTAAG	1 cut(s)	896
BstBAI	YACGTR	1 cut(s)	449
BstBI	TTCGAA	2 cut(s)	2287, 2447
BstC8I	GCNNGC	4 cut(s)	6, 36, 1892, 2349
BstDEI	CTNAG	7 cut(s)	825, 1425, 1671, 1723, 1860, 1994, 2556
BstEII	GGTNACC	2 cut(s)	353, 1675
BstENI	CCTNNNNNAGG	1 cut(s)	2484
BstF5I	GGATG	2 cut(s)	2079, 2370
BstH2I	RGCGCY	1 cut(s)	143
BstHHI	GCGC	1 cut(s)	142
BstMAI	GTCTC	4 cut(s)	860, 1339, 1439, 1471
BstMCI	CGRYCG	2 cut(s)	915, 1055
BstMWI	GCNNNNNNNGC	7 cut(s)	10, 725, 1016, 1751, 2006, 2040, 2406
BstNI	CCWGG	7 cut(s)	87, 1381, 2011, 2071, 2190, 2226, 2278
BstNSI	RCATGY	2 cut(s)	1039, 1772
BstPAI	GACNNNNGTC	1 cut(s)	973
BstPI	GGTNACC	2 cut(s)	353, 1675
BstSCI	CCNGG	8 cut(s)	85, 170, 1379, 2009, 2069, 2188, 2224, 2276
BstSLI	GKGCMC	1 cut(s)	99
BstV1I	GCAGC	6 cut(s)	737, 800, 1788, 1946, 2364, 2554
BstV2I	GAAGAC	1 cut(s)	1838
BstX2I	RGATCY	2 cut(s)	1251, 1273
BstXI	CCANNNNNNTGG	1 cut(s)	2051
BstYI	RGATCY	2 cut(s)	1251, 1273
Bsu15I	ATCGAT	2 cut(s)	911, 915
BsuRI	GGCC	6 cut(s)	184, 714, 1003, 1095, 2193, 2490
BsuTUI	ATCGAT	2 cut(s)	911, 915
BtsCI	GGATG	2 cut(s)	2079, 2370
BtsIMutI	CAGTG	2 cut(s)	160, 1337
Cac8I	GCNNGC	4 cut(s)	6, 36, 1892, 2349
CaiI	CAGNNNCTG	1 cut(s)	1871
CfoI	GCGC	1 cut(s)	142
Cfr10I	RCCGGY	1 cut(s)	4
Cfr13I	GGNCC	7 cut(s)	182, 1001, 1057, 1130, 2192, 2489, 2532
ClaI	ATCGAT	2 cut(s)	911, 915
CpoI	CGGWCCG	1 cut(s)	2532
CseI	GACGC	1 cut(s)	978
Csp6I	GTAC	5 cut(s)	450, 901, 1268, 2118, 2522
CspI	CGGWCCG	1 cut(s)	2532
CviAII	CATG	6 cut(s)	710, 959, 1036, 1580, 1769, 2125
CviQI	GTAC	5 cut(s)	450, 901, 1268, 2118, 2522
DdeI	CTNAG	7 cut(s)	825, 1425, 1671, 1723, 1860, 1994, 2556
DraIII	CACNNNGTG	1 cut(s)	1820
EaeI	YGGCCR	1 cut(s)	1093
Eam1104I	CTCTTC	3 cut(s)	18, 604, 2397
EarI	CTCTTC	3 cut(s)	18, 604, 2397
Eco130I	CCWWGG	3 cut(s)	99, 1156, 1501
Eco31I	GGTCTC	2 cut(s)	860, 1439
Eco32I	GATATC	2 cut(s)	737, 1307
Eco47I	GGWCC	3 cut(s)	1057, 1130, 2532
Eco57I	CTGAAG	2 cut(s)	964, 2378
Eco91I	GGTNACC	2 cut(s)	353, 1675
EcoNI	CCTNNNNNAGG	1 cut(s)	2484
EcoO109I	RGGNCCY	1 cut(s)	2489
EcoO65I	GGTNACC	2 cut(s)	353, 1675
EcoRI	GAATTC	1 cut(s)	2178
EcoRII	CCWGG	7 cut(s)	85, 1379, 2009, 2069, 2188, 2224, 2276
EcoRV	GATATC	2 cut(s)	737, 1307
EcoT14I	CCWWGG	3 cut(s)	99, 1156, 1501
EcoT22I	ATGCAT	1 cut(s)	1632
ErhI	CCWWGG	3 cut(s)	99, 1156, 1501
FaeI	CATG	6 cut(s)	713, 962, 1039, 1583, 1772, 2128
FalI	AAGNNNNNCTT	4 cut(s)	1294, 1326, 1800, 1832
FaqI	GGGAC	1 cut(s)	2164
FatI	CATG	6 cut(s)	709, 958, 1035, 1579, 1768, 2124
FauI	CCCGC	2 cut(s)	1639, 2141
FblI	GTMKAC	1 cut(s)	837
Fnu4HI	GCNGC	6 cut(s)	726, 814, 1802, 1935, 2378, 2543
FokI	GGATG	2 cut(s)	2086, 2377
Fsp4HI	GCNGC	6 cut(s)	726, 814, 1802, 1935, 2378, 2543
FspBI	CTAG	6 cut(s)	716, 2006, 2082, 2348, 2438, 2486
GlaI	GCGC	1 cut(s)	141
GluI	GCNGC	6 cut(s)	726, 814, 1802, 1935, 2378, 2543
GsaI	CCCAGC	2 cut(s)	953, 2577
GsuI	CTGGAG	4 cut(s)	108, 901, 1626, 2260
HaeII	RGCGCY	1 cut(s)	143
HaeIII	GGCC	6 cut(s)	184, 714, 1003, 1095, 2193, 2490
HapII	CCGG	3 cut(s)	5, 171, 1680
HgaI	GACGC	1 cut(s)	978
HhaI	GCGC	1 cut(s)	142
Hin1II	CATG	6 cut(s)	713, 962, 1039, 1583, 1772, 2128
Hin6I	GCGC	1 cut(s)	140
HinP1I	GCGC	1 cut(s)	140
HindIII	AAGCTT	1 cut(s)	764
HinfI	GANTC	7 cut(s)	1048, 1347, 1513, 1762, 1811, 2281, 2389
HpaII	CCGG	3 cut(s)	5, 171, 1680
HphI	GGTGA	6 cut(s)	551, 1354, 1672, 1687, 1889, 2085
Hpy166II	GTNNAC	8 cut(s)	838, 1130, 1321, 1663, 1790, 1823, 1849, 1945
Hpy188III	TCNNGA	9 cut(s)	268, 401, 749, 880, 1605, 2214, 2299, 2371, 2502
Hpy8I	GTNNAC	8 cut(s)	838, 1130, 1321, 1663, 1790, 1823, 1849, 1945
HpyAV	CCTTC	9 cut(s)	24, 851, 881, 988, 1584, 2008, 2283, 2490, 2502
HpyCH4III	ACNGT	9 cut(s)	133, 155, 499, 905, 1547, 1565, 1576, 1921, 2111
HpyCH4IV	ACGT	1 cut(s)	448
HpyF10VI	GCNNNNNNNGC	7 cut(s)	10, 725, 1016, 1751, 2006, 2040, 2406
HpyF3I	CTNAG	7 cut(s)	825, 1425, 1671, 1723, 1860, 1994, 2556
HpySE526I	ACGT	1 cut(s)	448
Hsp92II	CATG	6 cut(s)	713, 962, 1039, 1583, 1772, 2128
HspAI	GCGC	1 cut(s)	140
KpnI	GGTACC	1 cut(s)	2525
KroI	GCCGGC	1 cut(s)	4
KroNI	GCCGGC	1 cut(s)	6
LmnI	GCTCC	4 cut(s)	259, 1607, 1809, 2230
Lsp1109I	GCAGC	6 cut(s)	737, 800, 1788, 1946, 2364, 2554
LweI	GCATC	5 cut(s)	1438, 1639, 1858, 2009, 2364
MaeI	CTAG	6 cut(s)	716, 2006, 2082, 2348, 2438, 2486
MaeII	ACGT	1 cut(s)	448
MaeIII	GTNAC	8 cut(s)	353, 493, 1067, 1282, 1295, 1675, 2091, 2499
MboII	GAAGA	8 cut(s)	35, 591, 764, 935, 1607, 1843, 2276, 2384
MflI	RGATCY	2 cut(s)	1251, 1273
MhlI	GDGCHC	2 cut(s)	99, 319
MlyI	GAGTC	2 cut(s)	1341, 2383
Mph1103I	ATGCAT	1 cut(s)	1632
MroNI	GCCGGC	1 cut(s)	4
MroXI	GAANNNNTTC	1 cut(s)	253
MseI	TTAA	8 cut(s)	144, 333, 461, 897, 942, 1391, 1710, 2475
MslI	CAYNNNNRTG	2 cut(s)	560, 708
MspA1I	CMGCKG	2 cut(s)	1801, 2555
MspCI	CTTAAG	1 cut(s)	896
MspI	CCGG	3 cut(s)	5, 171, 1680
MspR9I	CCNGG	8 cut(s)	87, 172, 1381, 2011, 2071, 2190, 2226, 2278
Mva1269I	GAATGC	2 cut(s)	396, 2456
MvaI	CCWGG	7 cut(s)	87, 1381, 2011, 2071, 2190, 2226, 2278
MwoI	GCNNNNNNNGC	7 cut(s)	10, 725, 1016, 1751, 2006, 2040, 2406
NaeI	GCCGGC	1 cut(s)	6
NciI	CCSGG	1 cut(s)	172
NgoMIV	GCCGGC	1 cut(s)	4
NheI	GCTAGC	1 cut(s)	2347
NlaIII	CATG	6 cut(s)	713, 962, 1039, 1583, 1772, 2128
NlaIV	GGNNCC	4 cut(s)	261, 1132, 2194, 2523
NmuCI	GTSAC	4 cut(s)	493, 1675, 2091, 2499
NsiI	ATGCAT	1 cut(s)	1632
NspI	RCATGY	2 cut(s)	1039, 1772
NspV	TTCGAA	2 cut(s)	2287, 2447
PciI	ACATGT	1 cut(s)	1768
PcsI	WCGNNNNNNNCGW	1 cut(s)	652
PctI	GAATGC	2 cut(s)	396, 2456
PdiI	GCCGGC	1 cut(s)	6
PdmI	GAANNNNTTC	1 cut(s)	253
PfeI	GAWTC	5 cut(s)	1048, 1513, 1762, 1811, 2281
PfoI	TCCNGGA	2 cut(s)	2069, 2276
PkrI	GCNGC	6 cut(s)	727, 815, 1803, 1936, 2379, 2544
Ple19I	CGATCG	1 cut(s)	915
PleI	GAGTC	2 cut(s)	1341, 2383
PpsI	GAGTC	2 cut(s)	1341, 2383
Ppu21I	YACGTR	1 cut(s)	449
PscI	ACATGT	1 cut(s)	1768
PshAI	GACNNNNGTC	1 cut(s)	973
PshBI	ATTAAT	1 cut(s)	461
Psp6I	CCWGG	7 cut(s)	85, 1379, 2009, 2069, 2188, 2224, 2276
PspEI	GGTNACC	2 cut(s)	353, 1675
PspFI	CCCAGC	2 cut(s)	949, 2573
PspGI	CCWGG	7 cut(s)	85, 1379, 2009, 2069, 2188, 2224, 2276
PspN4I	GGNNCC	4 cut(s)	261, 1132, 2194, 2523
PspPI	GGNCC	7 cut(s)	182, 1001, 1057, 1130, 2192, 2489, 2532
PsrI	GAACNNNNNNTAC	2 cut(s)	1540, 1572
PstNI	CAGNNNCTG	1 cut(s)	1871
PsuI	RGATCY	2 cut(s)	1251, 1273
PvuI	CGATCG	1 cut(s)	915
PvuII	CAGCTG	2 cut(s)	1801, 2555
RsaI	GTAC	5 cut(s)	451, 902, 1269, 2119, 2523
RsaNI	GTAC	5 cut(s)	450, 901, 1268, 2118, 2522
RseI	CAYNNNNRTG	2 cut(s)	560, 708
Rsr2I	CGGWCCG	1 cut(s)	2532
RsrII	CGGWCCG	1 cut(s)	2532
SaqAI	TTAA	8 cut(s)	144, 333, 461, 897, 942, 1391, 1710, 2475
SatI	GCNGC	6 cut(s)	726, 814, 1802, 1935, 2378, 2543
Sau96I	GGNCC	7 cut(s)	182, 1001, 1057, 1130, 2192, 2489, 2532
ScaI	AGTACT	1 cut(s)	902
SchI	GAGTC	2 cut(s)	1341, 2383
ScrFI	CCNGG	8 cut(s)	87, 172, 1381, 2011, 2071, 2190, 2226, 2278
SduI	GDGCHC	2 cut(s)	99, 319
SfaNI	GCATC	5 cut(s)	1438, 1639, 1858, 2009, 2364
SfuI	TTCGAA	2 cut(s)	2287, 2447
SinI	GGWCC	3 cut(s)	1057, 1130, 2532
SmiMI	CAYNNNNRTG	2 cut(s)	560, 708
SmlI	CTYRAG	4 cut(s)	310, 401, 720, 896
SmoI	CTYRAG	4 cut(s)	310, 401, 720, 896
SsiI	CCGC	3 cut(s)	1646, 2148, 2535
SspI	AATATT	1 cut(s)	662
SspMI	CTAG	6 cut(s)	716, 2006, 2082, 2348, 2438, 2486
StyD4I	CCNGG	8 cut(s)	85, 170, 1379, 2009, 2069, 2188, 2224, 2276
StyI	CCWWGG	3 cut(s)	99, 1156, 1501
TaaI	ACNGT	9 cut(s)	133, 155, 499, 905, 1547, 1565, 1576, 1921, 2111
TaiI	ACGT	1 cut(s)	451
TaqI	TCGA	8 cut(s)	197, 655, 760, 860, 911, 915, 2287, 2447
TaqII	GACCGA	2 cut(s)	1041, 1045
TatI	WGTACW	1 cut(s)	900
TfiI	GAWTC	5 cut(s)	1048, 1513, 1762, 1811, 2281
Tru1I	TTAA	8 cut(s)	144, 333, 461, 897, 942, 1391, 1710, 2475
Tru9I	TTAA	8 cut(s)	144, 333, 461, 897, 942, 1391, 1710, 2475
TscAI	CASTG	2 cut(s)	160, 1344
TseFI	GTSAC	4 cut(s)	493, 1675, 2091, 2499
TseI	GCWGC	6 cut(s)	725, 813, 1801, 1934, 2377, 2542
Tsp45I	GTSAC	4 cut(s)	493, 1675, 2091, 2499
TspGWI	ACGGA	4 cut(s)	522, 981, 1160, 1211
TspRI	CASTG	2 cut(s)	160, 1344
Vha464I	CTTAAG	1 cut(s)	896
VpaK11BI	GGWCC	3 cut(s)	1057, 1130, 2532
VspI	ATTAAT	1 cut(s)	461
XagI	CCTNNNNNAGG	1 cut(s)	2484
XceI	RCATGY	2 cut(s)	1039, 1772
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1814, 2408
XmiI	GTMKAC	1 cut(s)	837
XmnI	GAANNNNTTC	1 cut(s)	253
XspI	CTAG	6 cut(s)	716, 2006, 2082, 2348, 2438, 2486
ZrmI	AGTACT	1 cut(s)	902
Zsp2I	ATGCAT	1 cut(s)	1632

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.