Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004268

Physical Location & Seq

Genomic Coordinates Reverse (-)

36946806 .. 36952641

Gene Structure 5836 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1292600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3009 bp

ATGGCGGTGGCAGGAGGGGGAACGGCATTGTCGTTTTCGGTTGCGTCCATGGTGGAGGACGTGCTTCAACAGCACGGCACTCGCCTCGGCGATCTTAAGTTGGAGTCTCGAAAAGCTGAGGAAGCAGCATCGAGGAGAAATGAGGCGGCCGGGTGGCTTCGAAAGATGATCGGCGTTGTTGCGGCTAAAGATATGCCGGCAGAGCCTTCGGAGGAAGAGTTCAGGCTTGCATTGAGAAGTGGGATTATCCTCTGCAATGCTATCAATAAAGTTCAACCTGGAGTTGTGCCCAAGGTGGTAGAAAGTCCATGTGATTCAGCATTGATTCCCGATGGGGCGGCTTTGTCTGCATTTCAGTACTTCGAGAATGTGAGGAACTTCCTTGTTGCTATTCAGGGGATGGGACTTCCCACGTTTGAGGCATCTGATTTGGAACAAGGAGGGAAATCTGCAAGGGTTGTGAATACGGTTCTGGCGCTTAAATCTTACAGTGAGGGGAAACAAACCGGGGGAAATGGGCCAAATGGCTCGAAATTTGGTGCAAACATAAAACCTACTACTTCTGCCAAGCCTTTTGTAAGAAAAAATTCGGAGCCATTCACGAATTCGCTGTCAAGGACTTCCTCAATGAATGACAAATCTTTGAGTGCTCTGTCCTCTGATCTTAATTCTAACAAGATGCCTAATTCTCAGTCCTTGAGTATGCTTGTTCGCGCAGTTTTATTAGATAAGAAGCCTGAAGAAGTTCCGATGTTGGTAGAGTCTGTGCTAAGCAAGCTCGTGGAGGAATTTGAGCAACGTATGTCCAGCCAATATGAATTGACAAAACCAAGTCAAAAAGATGTGGCTGTTTCACACGGAAACAAATTTCCGATGAAATTCGCTTCTGGTGATAAAAAGATGGAAGACAAAACACTTGTCAATAAAAATTACATATCTGACGAGGAATCAAAAAGTCGGCTCCTTAAACAGCAAATGATCTTTGATCAACAACAAAGAGATGTTCAGGAACTAAAGCAAACTCTTCACACTACCAAATCTGGTATTCAGTTTATGAAAATGAAGTTCCATGAGGAGTTCCACAATATTGGTTTGCACATTCACGGCCTAGCTCATGCAGCGAGTGGATATCACAGAGTTCTTGAAGAAAATCGAAAGCTTTACAATCAAGTCCAAGACCTCAAAGGAAGTATCAGGGTTTATTGTCGAGTGAGACCCTTCTTATCTGGAGTATCAAACTGCTTAAGTACTGTGGATCATATAGAAGATGGAAATATCACTATTAACACCCCAGCAAGGCATGGGAACGGACGCAAGTCCTTCAGTTTCAACAAAGTATTCGGGCCATCTGCAACCCAAGCTGATGTTTTCTCTGACATGCACCCATTGATTCGGTCGGTCCTTGATGGTTACAATGTTTGCATATTTGCATACGGCCAAACTGGATCGGGCAAAACTTACACTATGAGTGGACCCAGAGAGCTTACGGAGAAAAGCCAAGGTGTAAATTTTAGGGCATTGGGAGATTTGTTTTTTATAGCAGATCAAAGAAAGGACACTTTCCGTTATGATGTTTCTGTTCAAATGATTGAGATCTATAACGAGCAAGTACGAGATCTCCTTGTTACTGACGGGAAAATTCGTAATAGTTCCCAGACAGGTCTCAATGTGCCAGATGCAAATCTTGTTCCTGTGTCATCAACATCTGCTGTTATTGATCTGATGAACCTTGGACATAGGAATCGTGTTGTGGGTGCAACTGCCCTGAATGACCGTAGTAGTCGTTCTCACAGTTGCTTGACTGTTCATGTTCAAGGAAGGGATTTGACGTCTGGAGCTATTCTTCGTGGCTGTATGCATCTGGTTGATCTTGCGGGAAGTGAGAGGGTGGACAAATCTGAGGTGACCGGAGATAGACTAAAAGAAGCTCAACATATTAACAAATCCCTCTCAGCTTTAGGAGATGTGATCGCTTCCCTTGCCCAAAAGAATCCACATGTTCCATATAGAAATAGTAAACTAACACAGCTGCTCCAAGATTCACTTGGTGGACAGGCGAAGACACTTATGTTTGTTCACATAAGCCCTGAGCCAGATGCTGTTGGTGAAACAATAAGCACGCTCAAATTTGCAGAGCGAGTAGCCACAGTTGAACTTGGTGCAGCCCAAGTAAACAAAGATAGCACAGATGTCAAAGCTCTCAAAGAACAGATTGCTGGTCTTAGGGCATCACTAGCCAGGAAGGACGAGGAAGCTGAACGCAATCAACGCCAAGTCTGTGGCAGCTCTGATAAATCCAGGACGAAAGCTAGTGAGCTGTCACCATTTCATTTTAACCGTCAGGGTACAGACATGATGGGCGATCTGAATGGTTGCAGGACACCAATAGTAGGCAAAATCGAGCTTGACGACAATTCTGCTTCGAGGACAAAAAGACAAAGCTTTGATCTTGAAGAGCTACTAGGGAATTCATCACCCTGGCCCCCAGTTGATAGTCCTATCCAGAACTATGAGGAGGATGATAGAGACGTGAGCTCGGGCGAGTGGGTTGATAAGGTTATGGTGAACAAGCAAGAAGCAAGGAGGCTTGGAAATCCTTTAGGATCCTGGGGAGCAGACAATGGGAATTTATCTGATGGTTTCTACCAGAAATATCTCCAGGATTCTTCCAAGGTTTATCCAGACCAATCCTACAATATGTTCGTGGGAAGCAACAAGTTCAATGTTGCTAGCACCGATGAAATGGATGATCTTGATGCTGCCACGAGCGACTCTTCAGAGCCAGATTTTCTCTGGCAATTCAATCAGACTAAACTTACTAGCATAGGCAATGGCATTGCATCGAAAACCAAGAAACCTAACGGAAAGCCAATGAAAAGCCTAGAACCAAGGAAAAGTTTTAATCCTTCTCTAGGCCCTTCACGAAAACCTTTGAACGGGGTACCACATCGGACTGCAAGGCAGCCAGTTCCAGCTGAGATGAAACGAAAAGCTGGGAGTAGAAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006810 GO:0006928 GO:0007017 GO:0007018 GO:0007097 GO:0008017 GO:0008092 GO:0008150 GO:0008569 GO:0009987 GO:0015629 GO:0015630 GO:0015631 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031534 GO:0032991 GO:0042623 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043621 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0046907 GO:0051012 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051647 GO:0051649 GO:0051656 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CH Kinesin Microtub_bd

Protein Analysis

1003

Amino Acids

110.14

Weight (kDa)

8.75

Isoelectric Point (pI)

45.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CH	PF00307	45 - 163	5.8e-12	Calponin homology (CH) domain
Microtub_bd	PF16796	383 - 541	3.9e-43	Microtubule binding
Kinesin	PF00225	420 - 716	1.8e-105	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004268)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47500 AT2G47500
fragaria_vesca	FvH4_2g26160 FvH4_2g26160 FvH4_2g26160
malus_domestica	MD00G1140500.v1.1 MD03G1292600.v1.1
prunus_persica	Prupe.8G265600_v2.0.a1 Prupe.8G265600_v2.0.a1
pyrus_communis	pycom03g23180 pycom11g27320
rosa_chinensis	RchiOBHm_Chr5g0061681 RchiOBHm_Chr6g0294551 RchiOBHm_Chr6g0294561
rosa_laevigata	RLG00000003184 RLG00000011860
rosa_multiflora	Rmu_co8460257.1_g000001 Rmu_sc0004268.1_g000003 Rmu_sc0004268.1_g000004 Rmu_sc0042489.1_g000001 Rmu_ssc0000248.1_g000004
rosa_roxburghii	Rroxscaffold_3G00250060 Rroxscaffold_7G00173230 Rroxscaffold_7G00197400
rosa_rugosa	Rorug06G0246000
rosa_samantha	Rh6AG358300 Rh6BG365000 Rh6BG365100 Rh6CG372100 Rh6DG358900 Rh7DG248300
rosa_wichuraiana	Rw6G031250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1832
Acc65I	GGTACC	1 cut(s)	2941
AccB1I	GGYRCC	1 cut(s)	2941
AccII	CGCG	1 cut(s)	714
AciI	CCGC	5 cut(s)	5, 146, 182, 338, 1874
AclWI	GGATC	4 cut(s)	1263, 1453, 2598, 2611
AcoI	YGGCCR	2 cut(s)	147, 1435
AcuI	CTGAAG	3 cut(s)	759, 1306, 2760
AcyI	GRCGYC	1 cut(s)	1829
AdeI	CACNNNGTG	1 cut(s)	2048
AfaI	GTAC	5 cut(s)	359, 1249, 1611, 2347, 2943
AfiI	CCNNNNNNNGG	4 cut(s)	1296, 2390, 2912, 2936
AflII	CTTAAG	2 cut(s)	95, 1243
AflIII	ACRYGT	1 cut(s)	1996
AjiI	CACGTC	2 cut(s)	61, 2530
AjnI	CCWGG	6 cut(s)	277, 2237, 2297, 2477, 2606, 2658
AloI	GAACNNNNNNTCC	2 cut(s)	203, 235
Alw21I	GWGCWC	2 cut(s)	652, 2537
Alw26I	GTCTC	4 cut(s)	111, 1207, 1667, 2520
AlwI	GGATC	4 cut(s)	1263, 1453, 2598, 2611
AlwNI	CAGNNNCTG	1 cut(s)	2099
Ama87I	CYCGRG	1 cut(s)	2536
AoxI	GGCC	7 cut(s)	147, 518, 1105, 1343, 1435, 2480, 2914
ApeKI	GCWGC	7 cut(s)	125, 1118, 2029, 2162, 2283, 2759, 2962
ArsI	GACNNNNNNTTYG	4 cut(s)	596, 628, 903, 935
Asp700I	GAANNNNTTC	2 cut(s)	586, 744
Asp718I	GGTACC	1 cut(s)	2941
AspLEI	GCGC	2 cut(s)	478, 716
AspS9I	GGNCC	6 cut(s)	518, 1343, 1399, 1472, 2481, 2915
AsuC2I	CCSGG	2 cut(s)	151, 508
AsuHPI	GGTGA	6 cut(s)	902, 1915, 2117, 2313, 2466, 2575
AsuII	TTCGAA	1 cut(s)	160
AsuNHI	GCTAGC	1 cut(s)	2729
AvaI	CYCGRG	1 cut(s)	2536
AvaII	GGWCC	2 cut(s)	1399, 1472
BaeGI	GKGCMC	1 cut(s)	291
BamHI	GGATCC	1 cut(s)	2603
BanI	GGYRCC	1 cut(s)	2941
BanII	GRGCYC	1 cut(s)	2537
BauI	CACGAG	2 cut(s)	779, 2764
BbsI	GAAGAC	2 cut(s)	912, 2066
Bbv12I	GWGCWC	2 cut(s)	652, 2537
BbvCI	CCTCAGC	1 cut(s)	117
BbvI	GCAGC	7 cut(s)	137, 1130, 2016, 2174, 2295, 2746, 2974
BccI	CCATC	8 cut(s)	326, 394, 895, 1262, 1354, 1400, 2350, 2630
BceAI	ACGGC	4 cut(s)	39, 91, 1120, 1450
BcgI	CGANNNNNNTGC	8 cut(s)	62, 67, 96, 101, 189, 223, 2399, 2433
BciT130I	CCWGG	6 cut(s)	279, 2239, 2299, 2479, 2608, 2660
BclI	TGATCA	1 cut(s)	985
BcnI	CCSGG	2 cut(s)	151, 508
BcoDI	GTCTC	4 cut(s)	111, 1207, 1667, 2520
BfaI	CTAG	8 cut(s)	1109, 2234, 2310, 2462, 2730, 2820, 2882, 2912
BfoI	RGCGCY	1 cut(s)	479
BfrI	CTTAAG	2 cut(s)	95, 1243
BglII	AGATCT	2 cut(s)	1593, 1615
BlpI	GCTNAGC	1 cut(s)	770
BmcAI	AGTACT	2 cut(s)	359, 1249
Bme1390I	CCNGG	8 cut(s)	151, 279, 508, 2239, 2299, 2479, 2608, 2660
Bme18I	GGWCC	2 cut(s)	1399, 1472
BmeT110I	CYCGRG	1 cut(s)	2536
BmgBI	CACGTC	2 cut(s)	61, 2530
BmgT120I	GGNCC	6 cut(s)	518, 1343, 1399, 1472, 2481, 2915
BmiI	GGNNCC	6 cut(s)	594, 962, 1474, 2483, 2605, 2943
BmrFI	CCNGG	8 cut(s)	151, 279, 508, 2239, 2299, 2479, 2608, 2660
BmrI	ACTGGG	1 cut(s)	2480
BmsI	GCATC	9 cut(s)	137, 431, 669, 1666, 1867, 2086, 2237, 2746, 2849
BmtI	GCTAGC	1 cut(s)	2733
BmuI	ACTGGG	1 cut(s)	2480
BoxI	GACNNNNGTC	1 cut(s)	1315
BpiI	GAAGAC	2 cut(s)	912, 2066
BpmI	CTGGAG	4 cut(s)	300, 1248, 1854, 2642
Bpu10I	CCTNAGC	2 cut(s)	117, 2088
Bpu1102I	GCTNAGC	1 cut(s)	770
Bpu14I	TTCGAA	1 cut(s)	160
BpuEI	CTTGAG	1 cut(s)	718
BpuMI	CCSGG	2 cut(s)	151, 508
BsaBI	GATNNNNATC	2 cut(s)	1592, 1680
BsaHI	GRCGYC	1 cut(s)	1829
BsaI	GGTCTC	2 cut(s)	1207, 1667
BsaWI	WCCGGW	1 cut(s)	1907
BsaXI	ACNNNNNCTCC	6 cut(s)	203, 233, 1602, 1632, 2016, 2046
Bsc4I	CCNNNNNNNGG	4 cut(s)	1296, 2390, 2912, 2936
Bse118I	RCCGGY	1 cut(s)	196
Bse1I	ACTGG	3 cut(s)	1447, 2486, 2966
Bse3DI	GCAATG	3 cut(s)	262, 2835, 2836
Bse8I	GATNNNNATC	2 cut(s)	1592, 1680
BseBI	CCWGG	6 cut(s)	279, 2239, 2299, 2479, 2608, 2660
BseGI	GGATG	3 cut(s)	405, 2524, 2752
BseJI	GATNNNNATC	2 cut(s)	1592, 1680
BseLI	CCNNNNNNNGG	4 cut(s)	1296, 2390, 2912, 2936
BseMI	GCAATG	3 cut(s)	262, 2835, 2836
BseMII	CTCAG	6 cut(s)	108, 704, 1890, 1965, 2079, 2967
BseNI	ACTGG	3 cut(s)	1447, 2486, 2966
BseRI	GAGGAG	3 cut(s)	148, 1088, 2528
BseSI	GKGCMC	1 cut(s)	291
BseX3I	CGGCCG	1 cut(s)	147
BseXI	GCAGC	7 cut(s)	137, 1130, 2016, 2174, 2295, 2746, 2974
BseYI	CCCAGC	2 cut(s)	1291, 2993
BsgI	GTGCAG	1 cut(s)	2181
Bsh1236I	CGCG	1 cut(s)	714
Bsh1285I	CGRYCG	2 cut(s)	150, 1397
BshFI	GGCC	7 cut(s)	149, 520, 1107, 1345, 1437, 2482, 2916
BshNI	GGYRCC	1 cut(s)	2941
BsiEI	CGRYCG	2 cut(s)	150, 1397
BsiHKAI	GWGCWC	2 cut(s)	652, 2537
BsiHKCI	CYCGRG	1 cut(s)	2536
BsiSI	CCGG	4 cut(s)	150, 197, 507, 1908
BslFI	GGGAC	1 cut(s)	417
BslI	CCNNNNNNNGG	4 cut(s)	1296, 2390, 2912, 2936
BsmAI	GTCTC	4 cut(s)	111, 1207, 1667, 2520
BsmBI	CGTCTC	1 cut(s)	2520
BsmFI	GGGAC	1 cut(s)	417
BsnI	GGCC	7 cut(s)	149, 520, 1107, 1345, 1437, 2482, 2916
Bso31I	GGTCTC	2 cut(s)	1207, 1667
BsoBI	CYCGRG	1 cut(s)	2536
Bsp119I	TTCGAA	1 cut(s)	160
Bsp1286I	GDGCHC	3 cut(s)	291, 652, 2537
Bsp1720I	GCTNAGC	1 cut(s)	770
Bsp19I	CCATGG	1 cut(s)	48
BspACI	CCGC	5 cut(s)	5, 146, 182, 338, 1874
BspANI	GGCC	7 cut(s)	149, 520, 1107, 1345, 1437, 2482, 2916
BspCNI	CTCAG	6 cut(s)	109, 703, 1891, 1964, 2080, 2968
BspFNI	CGCG	1 cut(s)	714
BspLI	GGNNCC	6 cut(s)	594, 962, 1474, 2483, 2605, 2943
BspOI	GCTAGC	1 cut(s)	2733
BspPI	GGATC	4 cut(s)	1263, 1453, 2598, 2611
BspQI	GCTCTTC	1 cut(s)	2448
BspT104I	TTCGAA	1 cut(s)	160
BspT107I	GGYRCC	1 cut(s)	2941
BspTI	CTTAAG	2 cut(s)	95, 1243
BspTNI	GGTCTC	2 cut(s)	1207, 1667
BsrDI	GCAATG	3 cut(s)	262, 2835, 2836
BsrFI	RCCGGY	1 cut(s)	196
BsrI	ACTGG	3 cut(s)	1447, 2486, 2966
BssAI	RCCGGY	1 cut(s)	196
BssNI	GRCGYC	1 cut(s)	1829
BssSI	CACGAG	2 cut(s)	779, 2764
BssT1I	CCWWGG	6 cut(s)	48, 291, 1498, 1729, 2670, 2888
Bst2BI	CACGAG	2 cut(s)	779, 2764
Bst2UI	CCWGG	6 cut(s)	279, 2239, 2299, 2479, 2608, 2660
Bst4CI	ACNGT	8 cut(s)	469, 491, 1252, 1775, 1793, 1804, 2149, 2339
Bst6I	CTCTTC	4 cut(s)	210, 1029, 2448, 2779
BstACI	GRCGYC	1 cut(s)	1829
BstAFI	CTTAAG	2 cut(s)	95, 1243
BstBI	TTCGAA	1 cut(s)	160
BstC8I	GCNNGC	5 cut(s)	198, 228, 776, 2120, 2731
BstDEI	CTNAG	8 cut(s)	117, 690, 770, 1899, 1951, 2088, 2222, 2976
BstDSI	CCRYGG	1 cut(s)	48
BstEII	GGTNACC	1 cut(s)	1903
BstENI	CCTNNNNNAGG	1 cut(s)	2910
BstF5I	GGATG	3 cut(s)	405, 2524, 2752
BstFNI	CGCG	1 cut(s)	714
BstH2I	RGCGCY	1 cut(s)	479
BstHHI	GCGC	2 cut(s)	478, 716
BstMAI	GTCTC	4 cut(s)	111, 1207, 1667, 2520
BstMCI	CGRYCG	2 cut(s)	150, 1397
BstNI	CCWGG	6 cut(s)	279, 2239, 2299, 2479, 2608, 2660
BstNSI	RCATGY	2 cut(s)	1381, 2000
BstPAI	GACNNNNGTC	1 cut(s)	1315
BstPI	GGTNACC	1 cut(s)	1903
BstSCI	CCNGG	8 cut(s)	149, 277, 506, 2237, 2297, 2477, 2606, 2658
BstSLI	GKGCMC	1 cut(s)	291
BstUI	CGCG	1 cut(s)	714
BstV1I	GCAGC	7 cut(s)	137, 1130, 2016, 2174, 2295, 2746, 2974
BstV2I	GAAGAC	2 cut(s)	912, 2066
BstX2I	RGATCY	3 cut(s)	1593, 1615, 2603
BstXI	CCANNNNNNTGG	2 cut(s)	1088, 2279
BstYI	RGATCY	3 cut(s)	1593, 1615, 2603
BstZI	CGGCCG	1 cut(s)	147
BsuRI	GGCC	7 cut(s)	149, 520, 1107, 1345, 1437, 2482, 2916
BtgI	CCRYGG	1 cut(s)	48
BtrI	CACGTC	2 cut(s)	61, 2530
BtsCI	GGATG	3 cut(s)	405, 2524, 2752
BtsIMutI	CAGTG	1 cut(s)	496
Cac8I	GCNNGC	5 cut(s)	198, 228, 776, 2120, 2731
CaiI	CAGNNNCTG	1 cut(s)	2099
CfoI	GCGC	2 cut(s)	478, 716
Cfr10I	RCCGGY	1 cut(s)	196
Cfr13I	GGNCC	6 cut(s)	518, 1343, 1399, 1472, 2481, 2915
CseI	GACGC	2 cut(s)	33, 1320
Csp6I	GTAC	5 cut(s)	358, 1248, 1610, 2346, 2942
CviAII	CATG	9 cut(s)	49, 309, 1070, 1115, 1301, 1378, 1808, 1997, 2353
CviQI	GTAC	5 cut(s)	358, 1248, 1610, 2346, 2942
DdeI	CTNAG	8 cut(s)	117, 690, 770, 1899, 1951, 2088, 2222, 2976
DraIII	CACNNNGTG	1 cut(s)	2048
EaeI	YGGCCR	2 cut(s)	147, 1435
EagI	CGGCCG	1 cut(s)	147
Eam1104I	CTCTTC	4 cut(s)	210, 1029, 2448, 2779
EarI	CTCTTC	4 cut(s)	210, 1029, 2448, 2779
Ecl136II	GAGCTC	1 cut(s)	2535
EclXI	CGGCCG	1 cut(s)	147
Eco130I	CCWWGG	6 cut(s)	48, 291, 1498, 1729, 2670, 2888
Eco24I	GRGCYC	1 cut(s)	2537
Eco31I	GGTCTC	2 cut(s)	1207, 1667
Eco32I	GATATC	1 cut(s)	1130
Eco47I	GGWCC	2 cut(s)	1399, 1472
Eco52I	CGGCCG	1 cut(s)	147
Eco53kI	GAGCTC	1 cut(s)	2535
Eco57I	CTGAAG	3 cut(s)	759, 1306, 2760
Eco88I	CYCGRG	1 cut(s)	2536
Eco91I	GGTNACC	1 cut(s)	1903
EcoICRI	GAGCTC	1 cut(s)	2535
EcoNI	CCTNNNNNAGG	1 cut(s)	2910
EcoO109I	RGGNCCY	1 cut(s)	2915
EcoO65I	GGTNACC	1 cut(s)	1903
EcoRI	GAATTC	2 cut(s)	604, 2467
EcoRII	CCWGG	6 cut(s)	277, 2237, 2297, 2477, 2606, 2658
EcoRV	GATATC	1 cut(s)	1130
EcoT14I	CCWWGG	6 cut(s)	48, 291, 1498, 1729, 2670, 2888
EcoT22I	ATGCAT	1 cut(s)	1860
EcoT38I	GRGCYC	1 cut(s)	2537
ErhI	CCWWGG	6 cut(s)	48, 291, 1498, 1729, 2670, 2888
Esp3I	CGTCTC	1 cut(s)	2520
FaeI	CATG	9 cut(s)	52, 312, 1073, 1118, 1304, 1381, 1811, 2000, 2356
FalI	AAGNNNNNCTT	2 cut(s)	2028, 2060
FaqI	GGGAC	1 cut(s)	417
FatI	CATG	9 cut(s)	48, 308, 1069, 1114, 1300, 1377, 1807, 1996, 2352
FauI	CCCGC	1 cut(s)	1867
FbaI	TGATCA	1 cut(s)	985
FokI	GGATG	3 cut(s)	412, 2531, 2759
FriOI	GRGCYC	1 cut(s)	2537
FspBI	CTAG	8 cut(s)	1109, 2234, 2310, 2462, 2730, 2820, 2882, 2912
GlaI	GCGC	2 cut(s)	477, 715
GsaI	CCCAGC	2 cut(s)	1295, 2997
GsuI	CTGGAG	4 cut(s)	300, 1248, 1854, 2642
HaeII	RGCGCY	1 cut(s)	479
HaeIII	GGCC	7 cut(s)	149, 520, 1107, 1345, 1437, 2482, 2916
HapII	CCGG	4 cut(s)	150, 197, 507, 1908
HgaI	GACGC	2 cut(s)	33, 1320
HhaI	GCGC	2 cut(s)	478, 716
Hin1I	GRCGYC	1 cut(s)	1829
Hin1II	CATG	9 cut(s)	52, 312, 1073, 1118, 1304, 1381, 1811, 2000, 2356
Hin6I	GCGC	2 cut(s)	476, 714
HinP1I	GCGC	2 cut(s)	476, 714
HindIII	AAGCTT	2 cut(s)	1157, 2440
HpaII	CCGG	4 cut(s)	150, 197, 507, 1908
HphI	GGTGA	6 cut(s)	902, 1915, 2117, 2313, 2466, 2575
Hpy166II	GTNNAC	7 cut(s)	1472, 1891, 2018, 2051, 2077, 2173, 2566
Hpy8I	GTNNAC	7 cut(s)	1472, 1891, 2018, 2051, 2077, 2173, 2566
HpyAV	CCTTC	7 cut(s)	216, 1228, 1330, 1812, 2236, 2916, 2928
HpyCH4III	ACNGT	8 cut(s)	469, 491, 1252, 1775, 1793, 1804, 2149, 2339
HpyCH4IV	ACGT	5 cut(s)	60, 413, 799, 1829, 2529
HpyF3I	CTNAG	8 cut(s)	117, 690, 770, 1899, 1951, 2088, 2222, 2976
HpySE526I	ACGT	5 cut(s)	60, 413, 799, 1829, 2529
Hsp92I	GRCGYC	1 cut(s)	1829
Hsp92II	CATG	9 cut(s)	52, 312, 1073, 1118, 1304, 1381, 1811, 2000, 2356
HspAI	GCGC	2 cut(s)	476, 714
KpnI	GGTACC	1 cut(s)	2945
KroI	GCCGGC	1 cut(s)	196
KroNI	GCCGGC	1 cut(s)	198
Ksp22I	TGATCA	1 cut(s)	985
LguI	GCTCTTC	1 cut(s)	2448
LmnI	GCTCC	5 cut(s)	592, 966, 1835, 2037, 2612
Lsp1109I	GCAGC	7 cut(s)	137, 1130, 2016, 2174, 2295, 2746, 2974
LweI	GCATC	9 cut(s)	137, 431, 669, 1666, 1867, 2086, 2237, 2746, 2849
MaeI	CTAG	8 cut(s)	1109, 2234, 2310, 2462, 2730, 2820, 2882, 2912
MaeII	ACGT	5 cut(s)	60, 413, 799, 1829, 2529
MaeIII	GTNAC	4 cut(s)	1409, 1624, 1903, 2319
MflI	RGATCY	3 cut(s)	1593, 1615, 2603
MhlI	GDGCHC	3 cut(s)	291, 652, 2537
MlyI	GAGTC	3 cut(s)	113, 770, 2765
MmeI	TCCRAC	1 cut(s)	81
Mph1103I	ATGCAT	1 cut(s)	1860
MroNI	GCCGGC	1 cut(s)	196
MroXI	GAANNNNTTC	2 cut(s)	586, 744
MseI	TTAA	9 cut(s)	96, 480, 666, 966, 1244, 1284, 1938, 2334, 2901
MspA1I	CMGCKG	2 cut(s)	2029, 2975
MspCI	CTTAAG	2 cut(s)	95, 1243
MspI	CCGG	4 cut(s)	150, 197, 507, 1908
MspR9I	CCNGG	8 cut(s)	151, 279, 508, 2239, 2299, 2479, 2608, 2660
MvaI	CCWGG	6 cut(s)	279, 2239, 2299, 2479, 2608, 2660
MvnI	CGCG	1 cut(s)	714
NaeI	GCCGGC	1 cut(s)	198
NciI	CCSGG	2 cut(s)	151, 508
NcoI	CCATGG	1 cut(s)	48
NgoMIV	GCCGGC	1 cut(s)	196
NheI	GCTAGC	1 cut(s)	2729
NlaIII	CATG	9 cut(s)	52, 312, 1073, 1118, 1304, 1381, 1811, 2000, 2356
NlaIV	GGNNCC	6 cut(s)	594, 962, 1474, 2483, 2605, 2943
NmeAIII	GCCGAG	1 cut(s)	66
NmuCI	GTSAC	2 cut(s)	1903, 2319
NsiI	ATGCAT	1 cut(s)	1860
NspI	RCATGY	2 cut(s)	1381, 2000
NspV	TTCGAA	1 cut(s)	160
PciI	ACATGT	1 cut(s)	1996
PciSI	GCTCTTC	1 cut(s)	2448
PcsI	WCGNNNNNNNCGW	2 cut(s)	29, 2406
PdiI	GCCGGC	1 cut(s)	198
PdmI	GAANNNNTTC	2 cut(s)	586, 744
PfeI	GAWTC	8 cut(s)	314, 325, 947, 1390, 1741, 1990, 2039, 2663
PfoI	TCCNGGA	2 cut(s)	2297, 2658
PleI	GAGTC	3 cut(s)	112, 769, 2765
PpsI	GAGTC	3 cut(s)	112, 769, 2765
PscI	ACATGT	1 cut(s)	1996
PshAI	GACNNNNGTC	1 cut(s)	1315
Psp124BI	GAGCTC	1 cut(s)	2537
Psp6I	CCWGG	6 cut(s)	277, 2237, 2297, 2477, 2606, 2658
PspEI	GGTNACC	1 cut(s)	1903
PspFI	CCCAGC	2 cut(s)	1291, 2993
PspGI	CCWGG	6 cut(s)	277, 2237, 2297, 2477, 2606, 2658
PspN4I	GGNNCC	6 cut(s)	594, 962, 1474, 2483, 2605, 2943
PspPI	GGNCC	6 cut(s)	518, 1343, 1399, 1472, 2481, 2915
PsrI	GAACNNNNNNTAC	2 cut(s)	1768, 1800
PstNI	CAGNNNCTG	1 cut(s)	2099
PsuI	RGATCY	3 cut(s)	1593, 1615, 2603
PvuII	CAGCTG	2 cut(s)	2029, 2975
RsaI	GTAC	5 cut(s)	359, 1249, 1611, 2347, 2943
RsaNI	GTAC	5 cut(s)	358, 1248, 1610, 2346, 2942
SacI	GAGCTC	1 cut(s)	2537
SapI	GCTCTTC	1 cut(s)	2448
SaqAI	TTAA	9 cut(s)	96, 480, 666, 966, 1244, 1284, 1938, 2334, 2901
Sau96I	GGNCC	6 cut(s)	518, 1343, 1399, 1472, 2481, 2915
ScaI	AGTACT	2 cut(s)	359, 1249
SchI	GAGTC	3 cut(s)	113, 770, 2765
ScrFI	CCNGG	8 cut(s)	151, 279, 508, 2239, 2299, 2479, 2608, 2660
SduI	GDGCHC	3 cut(s)	291, 652, 2537
SfaNI	GCATC	9 cut(s)	137, 431, 669, 1666, 1867, 2086, 2237, 2746, 2849
SfuI	TTCGAA	1 cut(s)	160
SinI	GGWCC	2 cut(s)	1399, 1472
SmlI	CTYRAG	3 cut(s)	95, 697, 1243
SmoI	CTYRAG	3 cut(s)	95, 697, 1243
SsiI	CCGC	5 cut(s)	5, 146, 182, 338, 1874
SspI	AATATT	1 cut(s)	1087
SspMI	CTAG	8 cut(s)	1109, 2234, 2310, 2462, 2730, 2820, 2882, 2912
SstI	GAGCTC	1 cut(s)	2537
StyD4I	CCNGG	8 cut(s)	149, 277, 506, 2237, 2297, 2477, 2606, 2658
StyI	CCWWGG	6 cut(s)	48, 291, 1498, 1729, 2670, 2888
TaaI	ACNGT	8 cut(s)	469, 491, 1252, 1775, 1793, 1804, 2149, 2339
TaiI	ACGT	5 cut(s)	63, 416, 802, 1832, 2532
TaqII	GACCGA	2 cut(s)	1383, 1387
TatI	WGTACW	2 cut(s)	357, 1247
TauI	GCSGC	3 cut(s)	149, 185, 341
TfiI	GAWTC	8 cut(s)	314, 325, 947, 1390, 1741, 1990, 2039, 2663
Tru1I	TTAA	9 cut(s)	96, 480, 666, 966, 1244, 1284, 1938, 2334, 2901
Tru9I	TTAA	9 cut(s)	96, 480, 666, 966, 1244, 1284, 1938, 2334, 2901
TscAI	CASTG	1 cut(s)	496
TseFI	GTSAC	2 cut(s)	1903, 2319
TseI	GCWGC	7 cut(s)	125, 1118, 2029, 2162, 2283, 2759, 2962
Tsp45I	GTSAC	2 cut(s)	1903, 2319
TspGWI	ACGGA	5 cut(s)	873, 1323, 1502, 1553, 2877
TspRI	CASTG	1 cut(s)	496
Vha464I	CTTAAG	2 cut(s)	95, 1243
VpaK11BI	GGWCC	2 cut(s)	1399, 1472
XagI	CCTNNNNNAGG	1 cut(s)	2910
XceI	RCATGY	2 cut(s)	1381, 2000
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2042, 2790
XmnI	GAANNNNTTC	2 cut(s)	586, 744
XspI	CTAG	8 cut(s)	1109, 2234, 2310, 2462, 2730, 2820, 2882, 2912
ZraI	GACGTC	1 cut(s)	1830
ZrmI	AGTACT	2 cut(s)	359, 1249
Zsp2I	ATGCAT	1 cut(s)	1860

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.