Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0006142

Physical Location & Seq

Genomic Coordinates Reverse (-)

48311348 .. 48312640

Gene Structure 1293 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1202300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1293 bp

ATGGATGGTTACTTTAAAGAGCGAAAGGAGTCAGTTGCTCTGAACTTGGCCCAAGAGATGATGGAGAAAAATATAGGGTTTGATGTTGTTGCATACAATGTCTTAATGAATGGTCTATTGAGGCTTGGGAAGTATGAAGCAATATCTGTTTGTACTGGAATGAAAAAGTTGGGTTTGGCTCTTGACTATGCCACATACAATACCATGATTAATGCATTCTGTAGAGAGGGTGACGCTGAAAATGCTTTTAAACTCTGGCATGAGATGAAGTGTCAAGGGTTAATTCCAAACTCAAACACTTGTAACATTCTGATCCGAGGACTTTGCGATGCAAATGAGATTGAAAAAGCCATAGATGTCTTGAATGGAATGTTGTCTGTTGGTTCTCTCCCTACCTCATTTATTCATAGAATTCTGCTTGATGCATCTTCAAAGAGTAGAAGAGCTGATTCAATTTTGCAGATGCATCATAAGCTTGTATGTATGGGGCTTAACCTCAATCGGGATGTTTATAATAATCTCATCATTGTCTTGTGTAGGCTAGGTATGACCATGAAAGCCACTTTAGTGTTGAAAGAGATGATTGGAGGAGGATTTTTAGCAGATATTGATACTTACAATGCCTTTATATGTGGATATTGTAGAAGCAGCCATATGAAAAGGGCTTTTGCTACTTACTCGCAAATGTTGGCTGAGGGAGTTTCTCCAAATATTGAAACGTACAATCTTCTTTTAGGTGGACTTTCAAGTGCTGGTCTGATGACAGATGCGGAGGAGTTATTTGGTCAAATGAAGAACAGAGGTTTTGTTCCTAATGCTTCTACATATGATACTTTGGTTTCTGGTCATGGTAAGAAGGGAAATAAAAAGGAAGCTATAAGACTTTATTGTGAAATGGTAAGCAAGGGTTTTGTTCCCAAAACTAGCACTTACAATATCCTTATTAGTGATTTTGCTAAGGCGGGAAAGATGGGCCAAGCTAGGGAGCTTATGAATGAGATGCAGACAAGAGGGACCTCTCCTAACTCTTCAACTTACAACATACTAATTTGCGGCTGGTGTTGGCTATCAAAGCAGCCAGAGCTGGAGAGAAGTTTGAAAAAGTCATATCGGGCTGAGGCAAAAAGATTATTAACTGATATGAAAGACAAAGATTATGTTCCATGTGAAATTACCCTTCTATGCATCAGTTCTACCTTTGCTCGACCAGGAAAGAAGGCTGATGCTCAGAGGCTATTGAAGGAACTGTATAAGAAAAAGTCATATGACCAAGAAATGATGGGAGTAAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

431

Amino Acids

48.1

Weight (kDa)

8.95

Isoelectric Point (pI)

38.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	12 - 159	2.4e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	16 - 73	9.1e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	28 - 75	1.5e-10	PPR repeat family
TPR_24	PF23276	47 - 154	3.3e-08	Fungal tetratrico peptide repeats
PPR_3	PF13812	51 - 106	2e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	58 - 90	8.6e-11	PPR repeat
PPR_2	PF13041	63 - 110	8.4e-19	PPR repeat family
PPR	PF01535	65 - 93	1.2e-09	PPR repeat
PPR_1	PF12854	93 - 124	3.7e-06	PPR repeat
PPR_2	PF13041	96 - 142	1.2e-07	PPR repeat family
PPR_3	PF13812	190 - 246	2.4e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	202 - 249	1.3e-13	PPR repeat family
PPR_long	PF17177	215 - 349	4.7e-10	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	233 - 265	9.2e-07	PPR repeat
PPR_2	PF13041	236 - 283	1.8e-12	PPR repeat family
PPR	PF01535	240 - 269	1.4e-06	PPR repeat
PPR_3	PF13812	260 - 317	3.9e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	267 - 300	5.9e-07	PPR repeat
PPR_2	PF13041	271 - 320	2.3e-12	PPR repeat family
PPR	PF01535	274 - 304	3.8e-06	PPR repeat
PPR_3	PF13812	295 - 350	2.3e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	302 - 335	3.8e-07	PPR repeat
PPR_2	PF13041	306 - 354	1.9e-15	PPR repeat family
PPR	PF01535	309 - 338	4.1e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006142)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14770 AT5G14770 AT5G14770 AT5G14770 AT5G14770
fragaria_vesca	FvH4_7g02371
malus_domestica	MD00G1202300.v1.1 MD00G1202400.v1.1 MD03G1142100.v1.1 MD11G1161100.v1.1 MD11G1161200.v1.1
prunus_persica	Prupe.2G007100_v2.0.a1
pyrus_communis	pycom03g09690 pycom11g13400
rosa_chinensis	RchiOBHm_Chr1g0384651
rosa_laevigata	RLG00000008173 RLG00000026023
rosa_multiflora	Rmu_co8325115.1_g000001 Rmu_sc0012136.1_g000024
rosa_roxburghii	Rroxscaffold_4G00276490
rosa_rugosa	Rorug01G0450400
rosa_samantha	Rh1AG475000 Rh1BG433200 Rh1CG446400 Rh1CG446600 Rh1DG465700
rosa_wichuraiana	Rw1G039760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	513
AciI	CCGC	3 cut(s)	770, 962, 1053
AclWI	GGATC	1 cut(s)	307
AcsI	RAATTY	1 cut(s)	411
AfaI	GTAC	2 cut(s)	154, 722
AfiI	CCNNNNNNNGG	2 cut(s)	502, 982
AjnI	CCWGG	1 cut(s)	1207
AleI	CACNNNNGTG	1 cut(s)	566
AluBI	AGCT	6 cut(s)	446, 475, 875, 980, 988, 1084
AluI	AGCT	6 cut(s)	446, 475, 875, 980, 988, 1084
AlwI	GGATC	1 cut(s)	307
AoxI	GGCC	2 cut(s)	48, 973
ApeKI	GCWGC	2 cut(s)	648, 1075
ApoI	RAATTY	1 cut(s)	411
AseI	ATTAAT	1 cut(s)	210
AspS9I	GGNCC	3 cut(s)	49, 973, 1014
AsuHPI	GGTGA	1 cut(s)	242
AvaII	GGWCC	1 cut(s)	1014
BaeI	ACNNNNGTAYC	2 cut(s)	822, 855
BbvCI	CCTCAGC	2 cut(s)	693, 1116
BbvI	GCAGC	2 cut(s)	660, 1087
BccI	CCATC	3 cut(s)	55, 964, 1273
BcgI	CGANNNNNNTGC	2 cut(s)	306, 340
BciT130I	CCWGG	1 cut(s)	1209
BfaI	CTAG	3 cut(s)	542, 924, 981
BfmI	CTRYAG	1 cut(s)	220
BisI	GCNGC	3 cut(s)	649, 1054, 1076
BlsI	GCNGC	3 cut(s)	650, 1055, 1077
Bme1390I	CCNGG	1 cut(s)	1209
Bme18I	GGWCC	1 cut(s)	1014
BmgT120I	GGNCC	3 cut(s)	49, 973, 1014
BmiI	GGNNCC	1 cut(s)	1015
BmrFI	CCNGG	1 cut(s)	1209
BmsI	GCATC	9 cut(s)	319, 412, 434, 453, 475, 757, 990, 1194, 1213
BpmI	CTGGAG	1 cut(s)	1106
Bpu10I	CCTNAGC	3 cut(s)	693, 957, 1116
BsaJI	CCNNGG	1 cut(s)	316
Bsc4I	CCNNNNNNNGG	2 cut(s)	502, 982
Bse1I	ACTGG	1 cut(s)	160
BseBI	CCWGG	1 cut(s)	1209
BseDI	CCNNGG	1 cut(s)	316
BseGI	GGATG	2 cut(s)	10, 511
BseLI	CCNNNNNNNGG	2 cut(s)	502, 982
BseMII	CTCAG	3 cut(s)	684, 1107, 1241
BseNI	ACTGG	1 cut(s)	160
BseRI	GAGGAG	2 cut(s)	603, 788
BseXI	GCAGC	2 cut(s)	660, 1087
BshFI	GGCC	2 cut(s)	50, 975
BslFI	GGGAC	1 cut(s)	1027
BslI	CCNNNNNNNGG	2 cut(s)	502, 982
BsmFI	GGGAC	1 cut(s)	1027
BsmI	GAATGC	1 cut(s)	215
BsnI	GGCC	2 cut(s)	50, 975
Bsp143I	GATC	1 cut(s)	312
BspACI	CCGC	3 cut(s)	770, 962, 1053
BspANI	GGCC	2 cut(s)	50, 975
BspCNI	CTCAG	3 cut(s)	685, 1108, 1240
BspLI	GGNNCC	1 cut(s)	1015
BspPI	GGATC	1 cut(s)	307
BspQI	GCTCTTC	1 cut(s)	436
BsrI	ACTGG	1 cut(s)	160
BssECI	CCNNGG	1 cut(s)	316
BssMI	GATC	1 cut(s)	312
Bst2UI	CCWGG	1 cut(s)	1209
Bst4CI	ACNGT	1 cut(s)	1248
Bst6I	CTCTTC	2 cut(s)	436, 1033
BstDEI	CTNAG	4 cut(s)	693, 957, 1116, 1227
BstF5I	GGATG	2 cut(s)	10, 511
BstKTI	GATC	1 cut(s)	315
BstMBI	GATC	1 cut(s)	312
BstMWI	GCNNNNNNNGC	4 cut(s)	242, 472, 1072, 1081
BstNI	CCWGG	1 cut(s)	1209
BstSCI	CCNGG	1 cut(s)	1207
BstSFI	CTRYAG	1 cut(s)	220
BstV1I	GCAGC	2 cut(s)	660, 1087
BsuRI	GGCC	2 cut(s)	50, 975
BtgZI	GCGATG	1 cut(s)	342
BtsCI	GGATG	2 cut(s)	10, 511
Cfr13I	GGNCC	3 cut(s)	49, 973, 1014
CseI	GACGC	1 cut(s)	242
Csp6I	GTAC	2 cut(s)	153, 721
CviAII	CATG	5 cut(s)	205, 260, 553, 848, 1164
CviQI	GTAC	2 cut(s)	153, 721
DdeI	CTNAG	4 cut(s)	693, 957, 1116, 1227
DpnI	GATC	1 cut(s)	314
DpnII	GATC	1 cut(s)	312
DraI	TTTAAA	2 cut(s)	16, 250
Eam1104I	CTCTTC	2 cut(s)	436, 1033
EarI	CTCTTC	2 cut(s)	436, 1033
Eco47I	GGWCC	1 cut(s)	1014
EcoO109I	RGGNCCY	1 cut(s)	1014
EcoRI	GAATTC	1 cut(s)	411
EcoRII	CCWGG	1 cut(s)	1207
EcoT22I	ATGCAT	4 cut(s)	217, 427, 468, 1187
FaeI	CATG	5 cut(s)	208, 263, 556, 851, 1167
FaqI	GGGAC	1 cut(s)	1027
FatI	CATG	5 cut(s)	204, 259, 552, 847, 1163
FauI	CCCGC	1 cut(s)	955
FauNDI	CATATG	3 cut(s)	654, 826, 1264
Fnu4HI	GCNGC	3 cut(s)	649, 1054, 1076
FokI	GGATG	2 cut(s)	17, 518
Fsp4HI	GCNGC	3 cut(s)	649, 1054, 1076
FspBI	CTAG	3 cut(s)	542, 924, 981
GluI	GCNGC	3 cut(s)	649, 1054, 1076
GsuI	CTGGAG	1 cut(s)	1106
HaeIII	GGCC	2 cut(s)	50, 975
HgaI	GACGC	1 cut(s)	242
Hin1II	CATG	5 cut(s)	208, 263, 556, 851, 1167
HindIII	AAGCTT	1 cut(s)	473
HinfI	GANTC	2 cut(s)	29, 449
HphI	GGTGA	1 cut(s)	242
Hpy166II	GTNNAC	1 cut(s)	740
Hpy188I	TCNGA	5 cut(s)	42, 312, 317, 759, 1230
Hpy188III	TCNNGA	3 cut(s)	182, 361, 503
Hpy8I	GTNNAC	1 cut(s)	740
HpyAV	CCTTC	4 cut(s)	850, 1187, 1210, 1234
HpyCH4III	ACNGT	1 cut(s)	1248
HpyCH4IV	ACGT	1 cut(s)	719
HpyCH4V	TGCA	8 cut(s)	92, 215, 332, 425, 460, 466, 1003, 1185
HpyF10VI	GCNNNNNNNGC	4 cut(s)	242, 472, 1072, 1081
HpyF3I	CTNAG	4 cut(s)	693, 957, 1116, 1227
HpySE526I	ACGT	1 cut(s)	719
Hsp92II	CATG	5 cut(s)	208, 263, 556, 851, 1167
Kzo9I	GATC	1 cut(s)	312
LguI	GCTCTTC	1 cut(s)	436
LmnI	GCTCC	1 cut(s)	985
LpnPI	CCDG	9 cut(s)	141, 241, 738, 828, 1042, 1070, 1092, 1194, 1221
Lsp1109I	GCAGC	2 cut(s)	660, 1087
LweI	GCATC	9 cut(s)	319, 412, 434, 453, 475, 757, 990, 1194, 1213
MaeI	CTAG	3 cut(s)	542, 924, 981
MaeII	ACGT	1 cut(s)	719
MaeIII	GTNAC	3 cut(s)	8, 230, 302
MalI	GATC	1 cut(s)	314
MboI	GATC	1 cut(s)	312
MboII	GAAGA	5 cut(s)	420, 453, 719, 805, 1020
MluCI	AATT	5 cut(s)	282, 411, 453, 1047, 1170
MlyI	GAGTC	1 cut(s)	38
Mph1103I	ATGCAT	4 cut(s)	217, 427, 468, 1187
MseI	TTAA	7 cut(s)	15, 104, 210, 249, 281, 492, 1133
MslI	CAYNNNNRTG	1 cut(s)	566
MspR9I	CCNGG	1 cut(s)	1209
Mva1269I	GAATGC	1 cut(s)	215
MvaI	CCWGG	1 cut(s)	1209
MwoI	GCNNNNNNNGC	4 cut(s)	242, 472, 1072, 1081
NdeI	CATATG	3 cut(s)	654, 826, 1264
NdeII	GATC	1 cut(s)	312
NlaIII	CATG	5 cut(s)	208, 263, 556, 851, 1167
NlaIV	GGNNCC	1 cut(s)	1015
NmuCI	GTSAC	1 cut(s)	230
NsiI	ATGCAT	4 cut(s)	217, 427, 468, 1187
OliI	CACNNNNGTG	1 cut(s)	566
PciSI	GCTCTTC	1 cut(s)	436
PctI	GAATGC	1 cut(s)	215
PfeI	GAWTC	1 cut(s)	449
PkrI	GCNGC	3 cut(s)	650, 1055, 1077
PleI	GAGTC	1 cut(s)	37
PpsI	GAGTC	1 cut(s)	37
PpuMI	RGGWCCY	1 cut(s)	1014
PshBI	ATTAAT	1 cut(s)	210
PsiI	TTATAA	1 cut(s)	513
Psp5II	RGGWCCY	1 cut(s)	1014
Psp6I	CCWGG	1 cut(s)	1207
PspGI	CCWGG	1 cut(s)	1207
PspN4I	GGNNCC	1 cut(s)	1015
PspPI	GGNCC	3 cut(s)	49, 973, 1014
PspPPI	RGGWCCY	1 cut(s)	1014
RsaI	GTAC	2 cut(s)	154, 722
RsaNI	GTAC	2 cut(s)	153, 721
RseI	CAYNNNNRTG	1 cut(s)	566
SapI	GCTCTTC	1 cut(s)	436
SaqAI	TTAA	7 cut(s)	15, 104, 210, 249, 281, 492, 1133
SatI	GCNGC	3 cut(s)	649, 1054, 1076
Sau3AI	GATC	1 cut(s)	312
Sau96I	GGNCC	3 cut(s)	49, 973, 1014
SchI	GAGTC	1 cut(s)	38
ScrFI	CCNGG	1 cut(s)	1209
SfaNI	GCATC	9 cut(s)	319, 412, 434, 453, 475, 757, 990, 1194, 1213
SfcI	CTRYAG	1 cut(s)	220
SinI	GGWCC	1 cut(s)	1014
SmiMI	CAYNNNNRTG	1 cut(s)	566
Sse9I	AATT	5 cut(s)	282, 411, 453, 1047, 1170
SsiI	CCGC	3 cut(s)	770, 962, 1053
SspI	AATATT	1 cut(s)	712
SspMI	CTAG	3 cut(s)	542, 924, 981
StyD4I	CCNGG	1 cut(s)	1207
TaaI	ACNGT	1 cut(s)	1248
TaiI	ACGT	1 cut(s)	722
TaqI	TCGA	1 cut(s)	1204
TasI	AATT	5 cut(s)	282, 411, 453, 1047, 1170
TatI	WGTACW	1 cut(s)	152
TauI	GCSGC	1 cut(s)	1056
TfiI	GAWTC	1 cut(s)	449
Tru1I	TTAA	7 cut(s)	15, 104, 210, 249, 281, 492, 1133
Tru9I	TTAA	7 cut(s)	15, 104, 210, 249, 281, 492, 1133
TseFI	GTSAC	1 cut(s)	230
TseI	GCWGC	2 cut(s)	648, 1075
Tsp45I	GTSAC	1 cut(s)	230
VpaK11BI	GGWCC	1 cut(s)	1014
VspI	ATTAAT	1 cut(s)	210
XapI	RAATTY	1 cut(s)	411
XspI	CTAG	3 cut(s)	542, 924, 981
Zsp2I	ATGCAT	4 cut(s)	217, 427, 468, 1187

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.