MD00G1202400.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0006142
Physical Location & Seq
Reverse (-)
48312705 .. 48315859
3155 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1202400.v1.1.491

Sequence Viewer

Length: 1494 bp
ATGTTCTTCAGTAAGCTCAAACCCTTTCATATTGCTCCATCTTTGAGTTTCTCATCAGCCAAACTGCATTGTTCAATTCCCATGACCATTCAAAACCCACCAATGTCTTCTCCTTCCCATGTTCAAGGCTACAGTTCTGCCATCCCTCCATCCAAGACCCACCTCTATGCATCATTCTTCTGCACCCTTGTACACCTTTACTTGACATGTGGCAGATTTTCCAATGCTTCTGGTGCCTTCCGTTCTATGCGAAATCACGGCCTTGTTCCTGATTTGCCGCTTTGGAATCAACTTCTTTACCAATTCAATTCCTCAGGTTGGGTTTCTCAGGCTTTTGGGTTTTTGACTCAAATGGTGAAGAGGGATATACCAATCGATTCATATACTTGCAATACACTAGTTAAAGGGTTTTGTCAAATTGGGTTGGTAGAGTACGCAGCACGGGTTATGGATAATTTGGTGGATGGTGGAATTCCACAGGATGTTGTAGGTTTTAACACCTTGATTGCTGGACATTGCAAAGCTGGACAAGTTAGTCAGGCACTTGAATTGATGGACAGAATGGGTGGGGAGAGTCTGTCTCCTGATATTATTACTTATAATACTCTGATTCATGGGTTTTGCAGCACGGGTGATTTTGCAAGGGCCAAGAGTCTTATAGATATGATGTTGAGATCTCAGACAAATGAAGAATGCCCTCATGATGAAAGAGATGATGGCCAAAATCAGACTGAAGATAAGAATTTGAAACCAAACCTTATAACACACACCACACTTATTAGTTCTTATAGTAAGCGGCAAGGACTTGAAGAAGCTCTGTCTTTGTTTGAGGAAATGGTTATGAATGCCATTTATCCAGATGTAGTTGCTTATAGCTCCATTATAAATGGCATTTGCAAGTATGGGAGGCTATCAGAAGCCAAAGTGCTTTTAAGGGAGATGGAGGAAATGGGTGTGGATCCTAATCATATCTCCTATACTACCCTTGTGGATTTCTTGTTTAAGGCAGGCTCTTCCGTGGAAGCTCTTGCCCTTCAAAGCCAAATGGTTGTTGGGAAAGCTGTTGATGCTAAAGACCTGTTTCGAACAATTTCAAAGCTTGGCCTTGTTCCAAACTCCATCACCTACTCTGCGTTCATACATGGACTTTGCAATTTAGGAGACATGAATGATGCAGAATCTGTACTTAAAGAAATGGAGAAGCGCCACATTCTCCCAAATATTATTACTTATTCTTCAATGATAAATGGCTTCGTGAAGGAAGGAAAGCTTGGGGAGGCCATGAGTTTATTAAGGGAGATGGTGCAAAAGAATATCCTGCCAAATGCTTTTGTGTATGCAACACTAATTGACGGGTGCTTTAAGGCAGGTAAACAAGAGCTCGCTCTTGATCTTTACAATGAAATGAAAATGGGAGGAGTGGAGGATAACAATTTTGTACTTGATACTTTTTTGAACAACATGAAAAGGCGCAGAAGGATGGAGGAAAACTGA

Protein Analysis

498

Amino Acids

55.35

Weight (kDa)

6.03

Isoelectric Point (pI)

41.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 90 - 139 3.4e-07 PPR repeat family
PPR_3 PF13812 148 - 205 1.1e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 157 - 188 6.9e-09 PPR repeat
PPR_2 PF13041 161 - 208 2e-16 PPR repeat family
PPR_1 PF12854 193 - 223 8.6e-10 PPR repeat
PPR_2 PF13041 195 - 226 9.8e-07 PPR repeat family
PPR PF01535 198 - 226 3.5e-06 PPR repeat
PPR_3 PF13812 244 - 298 2.3e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 247 - 280 6.9e-07 PPR repeat
PPR_2 PF13041 251 - 300 2.1e-12 PPR repeat family
PPR_1 PF12854 285 - 315 1.6e-09 PPR repeat
PPR_2 PF13041 286 - 333 2.7e-13 PPR repeat family
PPR PF01535 289 - 319 3.9e-06 PPR repeat
PPR_long PF17177 357 - 460 5.6e-08 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 357 - 469 3.7e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 360 - 420 7.6e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 368 - 400 1.1e-10 PPR repeat
PPR_2 PF13041 371 - 420 4.1e-16 PPR repeat family
PPR PF01535 374 - 403 1.4e-06 PPR repeat
PPR_1 PF12854 403 - 435 5.8e-10 PPR repeat
PPR_2 PF13041 408 - 455 1.6e-13 PPR repeat family
PPR PF01535 409 - 439 6.6e-09 PPR repeat
PPR_3 PF13812 429 - 475 6.4e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 437 - 470 4e-06 PPR repeat
PPR_2 PF13041 441 - 483 6.3e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 600, 761, 884
AasI GACNNNNNNGTC 1 cut(s) 534
Acc36I ACCTGC 1 cut(s) 1358
AccB1I GGYRCC 1 cut(s) 233
AciI CCGC 2 cut(s) 278, 796
AclWI GGATC 2 cut(s) 953, 966
AcoI YGGCCR 1 cut(s) 718
AcsI RAATTY 2 cut(s) 471, 742
AcuI CTGAAG 1 cut(s) 753
AfaI GTAC 4 cut(s) 192, 434, 1185, 1440
AfiI CCNNNNNNNGG 1 cut(s) 318
AflIII ACRYGT 1 cut(s) 206
AhlI ACTAGT 1 cut(s) 397
AjuI GAANNNNNNNTTGG 2 cut(s) 1254, 1286
AluBI AGCT 9 cut(s) 16, 524, 815, 876, 1025, 1061, 1099, 1270, 1381
AluI AGCT 9 cut(s) 16, 524, 815, 876, 1025, 1061, 1099, 1270, 1381
Alw21I GWGCWC 1 cut(s) 1383
Alw26I GTCTC 2 cut(s) 585, 1155
AlwI GGATC 2 cut(s) 953, 966
AlwNI CAGNNNCTG 1 cut(s) 1181
AoxI GGCC 5 cut(s) 259, 645, 718, 1102, 1278
ApeKI GCWGC 2 cut(s) 437, 624
ApoI RAATTY 2 cut(s) 471, 742
Asp700I GAANNNNTTC 1 cut(s) 1090
AspLEI GCGC 2 cut(s) 1206, 1473
AspS9I GGNCC 1 cut(s) 645
AsuHPI GGTGA 3 cut(s) 367, 644, 1114
AsuII TTCGAA 1 cut(s) 1084
AxyI CCTNAGG 1 cut(s) 313
BalI TGGCCA 1 cut(s) 720
BamHI GGATCC 1 cut(s) 958
BanI GGYRCC 1 cut(s) 233
BanII GRGCYC 1 cut(s) 1383
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 1383
BbvI GCAGC 2 cut(s) 449, 636
BceAI ACGGC 1 cut(s) 274
BcoDI GTCTC 2 cut(s) 585, 1155
BcuI ACTAGT 1 cut(s) 397
BfaI CTAG 1 cut(s) 398
BfmI CTRYAG 1 cut(s) 130
BfoI RGCGCY 1 cut(s) 1207
BfuAI ACCTGC 1 cut(s) 1358
BglII AGATCT 1 cut(s) 674
BisI GCNGC 4 cut(s) 278, 438, 625, 797
BlsI GCNGC 4 cut(s) 279, 439, 626, 798
BmgT120I GGNCC 1 cut(s) 645
BmiI GGNNCC 2 cut(s) 235, 960
BmsI GCATC 3 cut(s) 179, 1057, 1162
BpiI GAAGAC 1 cut(s) 99
BplI GAGNNNNNCTC 2 cut(s) 565, 597
Bpu14I TTCGAA 1 cut(s) 1084
Bsa29I ATCGAT 1 cut(s) 375
BsaBI GATNNNNATC 1 cut(s) 963
BsaJI CCNNGG 1 cut(s) 1017
BsaXI ACNNNNNCTCC 2 cut(s) 563, 593
Bsc4I CCNNNNNNNGG 1 cut(s) 318
Bse21I CCTNAGG 1 cut(s) 313
Bse3DI GCAATG 1 cut(s) 514
Bse8I GATNNNNATC 1 cut(s) 963
BseCI ATCGAT 1 cut(s) 375
BseDI CCNNGG 1 cut(s) 1017
BseGI GGATG 5 cut(s) 141, 149, 469, 487, 1485
BseJI GATNNNNATC 1 cut(s) 963
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMI GCAATG 1 cut(s) 514
BseMII CTCAG 3 cut(s) 327, 341, 692
BseRI GAGGAG 1 cut(s) 1431
BseXI GCAGC 2 cut(s) 449, 636
BsgI GTGCAG 1 cut(s) 166
BshFI GGCC 5 cut(s) 261, 647, 720, 1104, 1280
BshNI GGYRCC 1 cut(s) 233
BshVI ATCGAT 1 cut(s) 375
BsiHKAI GWGCWC 1 cut(s) 1383
BslI CCNNNNNNNGG 1 cut(s) 318
BsmAI GTCTC 2 cut(s) 585, 1155
BsmI GAATGC 2 cut(s) 698, 850
BsnI GGCC 5 cut(s) 261, 647, 720, 1104, 1280
Bsp119I TTCGAA 1 cut(s) 1084
Bsp1286I GDGCHC 1 cut(s) 1383
Bsp1407I TGTACA 1 cut(s) 190
Bsp143I GATC 3 cut(s) 674, 958, 1390
BspACI CCGC 2 cut(s) 278, 796
BspANI GGCC 5 cut(s) 261, 647, 720, 1104, 1280
BspCNI CTCAG 3 cut(s) 326, 340, 691
BspDI ATCGAT 1 cut(s) 375
BspHI TCATGA 1 cut(s) 700
BspLI GGNNCC 2 cut(s) 235, 960
BspMI ACCTGC 1 cut(s) 1358
BspPI GGATC 2 cut(s) 953, 966
BspQI GCTCTTC 1 cut(s) 1018
BspT104I TTCGAA 1 cut(s) 1084
BspT107I GGYRCC 1 cut(s) 233
BsrDI GCAATG 1 cut(s) 514
BsrGI TGTACA 1 cut(s) 190
BssECI CCNNGG 1 cut(s) 1017
BssMI GATC 3 cut(s) 674, 958, 1390
Bst4CI ACNGT 1 cut(s) 134
Bst6I CTCTTC 2 cut(s) 353, 1018
BstAUI TGTACA 1 cut(s) 190
BstBI TTCGAA 1 cut(s) 1084
BstC8I GCNNGC 2 cut(s) 1009, 1383
BstDEI CTNAG 3 cut(s) 313, 327, 678
BstDSI CCRYGG 1 cut(s) 1017
BstF5I GGATG 5 cut(s) 141, 149, 469, 487, 1485
BstH2I RGCGCY 1 cut(s) 1207
BstHHI GCGC 2 cut(s) 1206, 1473
BstKTI GATC 3 cut(s) 677, 961, 1393
BstMAI GTCTC 2 cut(s) 585, 1155
BstMBI GATC 3 cut(s) 674, 958, 1390
BstMWI GCNNNNNNNGC 2 cut(s) 233, 1067
BstNSI RCATGY 1 cut(s) 210
BstSFI CTRYAG 1 cut(s) 130
BstV1I GCAGC 2 cut(s) 449, 636
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 2 cut(s) 674, 958
BstYI RGATCY 2 cut(s) 674, 958
Bsu15I ATCGAT 1 cut(s) 375
Bsu36I CCTNAGG 1 cut(s) 313
BsuRI GGCC 5 cut(s) 261, 647, 720, 1104, 1280
BsuTUI ATCGAT 1 cut(s) 375
BtgI CCRYGG 1 cut(s) 1017
BtsCI GGATG 5 cut(s) 141, 149, 469, 487, 1485
BveI ACCTGC 1 cut(s) 1358
Cac8I GCNNGC 2 cut(s) 1009, 1383
CaiI CAGNNNCTG 1 cut(s) 1181
CciI TCATGA 1 cut(s) 700
CfoI GCGC 2 cut(s) 1206, 1473
Cfr13I GGNCC 1 cut(s) 645
ClaI ATCGAT 1 cut(s) 375
Csp6I GTAC 4 cut(s) 191, 433, 1184, 1439
CviAII CATG 9 cut(s) 82, 119, 207, 614, 701, 1142, 1165, 1282, 1462
CviQI GTAC 4 cut(s) 191, 433, 1184, 1439
DdeI CTNAG 3 cut(s) 313, 327, 678
DpnI GATC 3 cut(s) 676, 960, 1392
DpnII GATC 3 cut(s) 674, 958, 1390
DrdI GACNNNNNNGTC 1 cut(s) 534
DseDI GACNNNNNNGTC 1 cut(s) 534
EaeI YGGCCR 1 cut(s) 718
Eam1104I CTCTTC 2 cut(s) 353, 1018
EarI CTCTTC 2 cut(s) 353, 1018
Ecl136II GAGCTC 1 cut(s) 1381
Eco24I GRGCYC 1 cut(s) 1383
Eco53kI GAGCTC 1 cut(s) 1381
Eco57I CTGAAG 1 cut(s) 753
Eco81I CCTNAGG 1 cut(s) 313
EcoICRI GAGCTC 1 cut(s) 1381
EcoRI GAATTC 1 cut(s) 471
EcoT22I ATGCAT 1 cut(s) 172
EcoT38I GRGCYC 1 cut(s) 1383
FaeI CATG 9 cut(s) 85, 122, 210, 617, 704, 1145, 1168, 1285, 1465
FalI AAGNNNNNCTT 2 cut(s) 1254, 1286
FatI CATG 9 cut(s) 81, 118, 206, 613, 700, 1141, 1164, 1281, 1461
Fnu4HI GCNGC 4 cut(s) 278, 438, 625, 797
FokI GGATG 4 cut(s) 128, 136, 476, 494
FriOI GRGCYC 1 cut(s) 1383
Fsp4HI GCNGC 4 cut(s) 278, 438, 625, 797
FspBI CTAG 1 cut(s) 398
GlaI GCGC 2 cut(s) 1205, 1472
GluI GCNGC 4 cut(s) 278, 438, 625, 797
HaeII RGCGCY 1 cut(s) 1207
HaeIII GGCC 5 cut(s) 261, 647, 720, 1104, 1280
HhaI GCGC 2 cut(s) 1206, 1473
Hin1II CATG 9 cut(s) 85, 122, 210, 617, 704, 1145, 1168, 1285, 1465
Hin6I GCGC 2 cut(s) 1204, 1471
HinP1I GCGC 2 cut(s) 1204, 1471
HindIII AAGCTT 2 cut(s) 1097, 1268
HinfI GANTC 7 cut(s) 286, 346, 377, 574, 610, 652, 1178
HphI GGTGA 3 cut(s) 367, 644, 1114
Hpy166II GTNNAC 2 cut(s) 193, 1373
Hpy188I TCNGA 4 cut(s) 609, 681, 729, 916
Hpy188III TCNNGA 6 cut(s) 269, 584, 701, 857, 1255, 1388
Hpy8I GTNNAC 2 cut(s) 193, 1373
HpyAV CCTTC 6 cut(s) 123, 247, 1043, 1252, 1256, 1470
HpyCH4III ACNGT 1 cut(s) 134
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 1067
HpyF3I CTNAG 3 cut(s) 313, 327, 678
Hsp92II CATG 9 cut(s) 85, 122, 210, 617, 704, 1145, 1168, 1285, 1465
HspAI GCGC 2 cut(s) 1204, 1471
Kzo9I GATC 3 cut(s) 674, 958, 1390
LguI GCTCTTC 1 cut(s) 1018
LmnI GCTCC 2 cut(s) 40, 881
Lsp1109I GCAGC 2 cut(s) 449, 636
LweI GCATC 3 cut(s) 179, 1057, 1162
MaeI CTAG 1 cut(s) 398
MalI GATC 3 cut(s) 676, 960, 1392
MboI GATC 3 cut(s) 674, 958, 1390
MboII GAAGA 8 cut(s) 99, 169, 370, 701, 746, 821, 1005, 1227
MflI RGATCY 2 cut(s) 674, 958
MhlI GDGCHC 1 cut(s) 1383
MlsI TGGCCA 1 cut(s) 720
MluNI TGGCCA 1 cut(s) 720
MlyI GAGTC 3 cut(s) 340, 583, 661
Mox20I TGGCCA 1 cut(s) 720
Mph1103I ATGCAT 1 cut(s) 172
MroXI GAANNNNTTC 1 cut(s) 1090
MscI TGGCCA 1 cut(s) 720
MseI TTAA 7 cut(s) 402, 495, 932, 1002, 1188, 1292, 1362
MslI CAYNNNNRTG 1 cut(s) 165
Msp20I TGGCCA 1 cut(s) 720
Mva1269I GAATGC 2 cut(s) 698, 850
MwoI GCNNNNNNNGC 2 cut(s) 233, 1067
NdeII GATC 3 cut(s) 674, 958, 1390
NlaIII CATG 9 cut(s) 85, 122, 210, 617, 704, 1145, 1168, 1285, 1465
NlaIV GGNNCC 2 cut(s) 235, 960
NsiI ATGCAT 1 cut(s) 172
NspI RCATGY 1 cut(s) 210
NspV TTCGAA 1 cut(s) 1084
PagI TCATGA 1 cut(s) 700
PciI ACATGT 1 cut(s) 206
PciSI GCTCTTC 1 cut(s) 1018
PctI GAATGC 2 cut(s) 698, 850
PdmI GAANNNNTTC 1 cut(s) 1090
PfeI GAWTC 4 cut(s) 286, 377, 610, 1178
PkrI GCNGC 4 cut(s) 279, 439, 626, 798
PleI GAGTC 3 cut(s) 340, 582, 660
PpsI GAGTC 3 cut(s) 340, 582, 660
PscI ACATGT 1 cut(s) 206
PsiI TTATAA 3 cut(s) 600, 761, 884
Psp124BI GAGCTC 1 cut(s) 1383
PspN4I GGNNCC 2 cut(s) 235, 960
PspPI GGNCC 1 cut(s) 645
PstNI CAGNNNCTG 1 cut(s) 1181
PsuI RGATCY 2 cut(s) 674, 958
RsaI GTAC 4 cut(s) 192, 434, 1185, 1440
RsaNI GTAC 4 cut(s) 191, 433, 1184, 1439
RseI CAYNNNNRTG 1 cut(s) 165
SacI GAGCTC 1 cut(s) 1383
SapI GCTCTTC 1 cut(s) 1018
SaqAI TTAA 7 cut(s) 402, 495, 932, 1002, 1188, 1292, 1362
SatI GCNGC 4 cut(s) 278, 438, 625, 797
Sau3AI GATC 3 cut(s) 674, 958, 1390
Sau96I GGNCC 1 cut(s) 645
SchI GAGTC 3 cut(s) 340, 583, 661
SduI GDGCHC 1 cut(s) 1383
SfaNI GCATC 3 cut(s) 179, 1057, 1162
SfcI CTRYAG 1 cut(s) 130
SfuI TTCGAA 1 cut(s) 1084
SmiMI CAYNNNNRTG 1 cut(s) 165
SpeI ACTAGT 1 cut(s) 397
SsiI CCGC 2 cut(s) 278, 796
SspI AATATT 1 cut(s) 1222
SspMI CTAG 1 cut(s) 398
SstI GAGCTC 1 cut(s) 1383
TaaI ACNGT 1 cut(s) 134
TaqI TCGA 2 cut(s) 375, 1084
TatI WGTACW 3 cut(s) 190, 1183, 1438
TauI GCSGC 2 cut(s) 280, 799
TfiI GAWTC 4 cut(s) 286, 377, 610, 1178
Tru1I TTAA 7 cut(s) 402, 495, 932, 1002, 1188, 1292, 1362
Tru9I TTAA 7 cut(s) 402, 495, 932, 1002, 1188, 1292, 1362
TseI GCWGC 2 cut(s) 437, 624
TspGWI ACGGA 2 cut(s) 230, 1006
XapI RAATTY 2 cut(s) 471, 742
XceI RCATGY 1 cut(s) 210
XcmI CCANNNNNNNNNTGG 1 cut(s) 1049
XmnI GAANNNNTTC 1 cut(s) 1090
XspI CTAG 1 cut(s) 398
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.