RLG00000008173

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0006142
Physical Location & Seq
Forward (+)
26624408 .. 26625166
759 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008173

Sequence Viewer

Length: 654 bp
ATGGATGGAGTCCAGCTGAATGTTATAACCTTTAACTCTTTAATAGATGCTCTTTGTAAGGAGAGAAGGACCAAGGACGCCCTCGCTGCTTTACGGCTAATGACTCAAAGAGGTCTAAGTCCTGATGTGATCACTTACAACTGTCTAGTTCATGGATTATGCTCTTTGCAAGGAGCTGAGAGAGGGACCAATGACACCCTCACTGTTTTACTGCTAATGGCTGAAAGAGGTCTAAGTCCTGATGTTATCACTTACAACTGTCTAATTTATGGCATGTGCAATACATCACAATTGGGTGAAGCCACAAGGTTGTATGATGAAACGGTTGGTCTAGGCATCTTACCTGATATGATAACTTTGACAGTACTTTTCGATGCTCTCTGCAAAGAAGGGATGACAGAAGAGGCTCGCAAAGCAGTTGAGGCAACATTTCAATATGGCATGGAGGTTAGCACGATCACATTCGACATGCTGCTTCATCATTATTGTTTGCGTGGAAAAATTGACAAGGCAGAGAAAGTCATTAGTTTTATGGTGTTGAAGGGTCATGTTCCTGATACTGCCTCCTGTACAACCTTGGACAATAACTTTGTGAAAGTTAACAGTATAGATGAAGCTTTGAGGGTTGTTAAGGAAATGATCCAAAAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

218

Amino Acids

23.99

Weight (kDa)

5.25

Isoelectric Point (pI)

25.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 2 - 34 3.5e-09 PPR repeat
PPR_3 PF13812 2 - 50 4.6e-10 Pentatricopeptide repeat domain
TPR_24 PF23276 3 - 107 9.1e-09 Fungal tetratrico peptide repeats
PPR_2 PF13041 7 - 54 7e-16 PPR repeat family
PPR PF01535 9 - 39 8.1e-06 PPR repeat
PPR_1 PF12854 37 - 55 6.6e-07 PPR repeat
PPR_1 PF12854 76 - 107 1e-08 PPR repeat
PPR_2 PF13041 80 - 129 2.3e-13 PPR repeat family
PPR_2 PF13041 151 - 195 4.1e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 26
AclWI GGATC 1 cut(s) 634
AcyI GRCGYC 1 cut(s) 78
AfaI GTAC 2 cut(s) 366, 571
AgsI TTSAA 2 cut(s) 434, 541
AluBI AGCT 3 cut(s) 16, 176, 617
AluI AGCT 3 cut(s) 16, 176, 617
AlwI GGATC 1 cut(s) 634
ApeKI GCWGC 2 cut(s) 86, 472
ArsI GACNNNNNNTTYG 2 cut(s) 572, 604
AspS9I GGNCC 2 cut(s) 69, 186
AsuHPI GGTGA 1 cut(s) 308
AvaII GGWCC 2 cut(s) 69, 186
BbvI GCAGC 2 cut(s) 73, 459
BceAI ACGGC 1 cut(s) 110
BclI TGATCA 1 cut(s) 129
BfaI CTAG 2 cut(s) 146, 332
BisI GCNGC 2 cut(s) 87, 473
BlsI GCNGC 2 cut(s) 88, 474
BmcAI AGTACT 1 cut(s) 366
Bme18I GGWCC 2 cut(s) 69, 186
BmgT120I GGNCC 2 cut(s) 69, 186
BmiI GGNNCC 1 cut(s) 187
BmsI GCATC 3 cut(s) 37, 345, 364
BsaHI GRCGYC 1 cut(s) 78
BsaJI CCNNGG 2 cut(s) 72, 576
BseDI CCNNGG 2 cut(s) 72, 576
BseGI GGATG 2 cut(s) 10, 399
BseMII CTCAG 1 cut(s) 168
BseXI GCAGC 2 cut(s) 73, 459
BslFI GGGAC 1 cut(s) 199
BsmFI GGGAC 1 cut(s) 199
Bsp1407I TGTACA 1 cut(s) 569
Bsp143I GATC 3 cut(s) 129, 456, 639
BspCNI CTCAG 1 cut(s) 169
BspLI GGNNCC 1 cut(s) 187
BspPI GGATC 1 cut(s) 634
BsrGI TGTACA 1 cut(s) 569
BssECI CCNNGG 2 cut(s) 72, 576
BssMI GATC 3 cut(s) 129, 456, 639
BssNI GRCGYC 1 cut(s) 78
BssT1I CCWWGG 2 cut(s) 72, 576
Bst4CI ACNGT 6 cut(s) 143, 205, 260, 325, 364, 605
Bst6I CTCTTC 1 cut(s) 396
BstACI GRCGYC 1 cut(s) 78
BstAUI TGTACA 1 cut(s) 569
BstC8I GCNNGC 1 cut(s) 409
BstDEI CTNAG 3 cut(s) 116, 177, 233
BstF5I GGATG 2 cut(s) 10, 399
BstKTI GATC 3 cut(s) 132, 459, 642
BstMBI GATC 3 cut(s) 129, 456, 639
BstMWI GCNNNNNNNGC 3 cut(s) 86, 413, 422
BstNSI RCATGY 2 cut(s) 277, 472
BstV1I GCAGC 2 cut(s) 73, 459
BtsCI GGATG 2 cut(s) 10, 399
BtsIMutI CAGTG 1 cut(s) 201
Cac8I GCNNGC 1 cut(s) 409
Cfr13I GGNCC 2 cut(s) 69, 186
CseI GACGC 1 cut(s) 86
Csp6I GTAC 2 cut(s) 365, 570
CviAII CATG 5 cut(s) 152, 274, 442, 469, 548
CviJI RGCY 7 cut(s) 16, 97, 176, 221, 302, 407, 617
CviKI_1 RGCY 7 cut(s) 16, 97, 176, 221, 302, 407, 617
CviQI GTAC 2 cut(s) 365, 570
DdeI CTNAG 3 cut(s) 116, 177, 233
DpnI GATC 3 cut(s) 131, 458, 641
DpnII GATC 3 cut(s) 129, 456, 639
Eam1104I CTCTTC 1 cut(s) 396
EarI CTCTTC 1 cut(s) 396
Eco130I CCWWGG 2 cut(s) 72, 576
Eco47I GGWCC 2 cut(s) 69, 186
EcoT14I CCWWGG 2 cut(s) 72, 576
ErhI CCWWGG 2 cut(s) 72, 576
FaeI CATG 5 cut(s) 155, 277, 445, 472, 551
FaqI GGGAC 1 cut(s) 199
FatI CATG 5 cut(s) 151, 273, 441, 468, 547
FbaI TGATCA 1 cut(s) 129
Fnu4HI GCNGC 2 cut(s) 87, 473
FokI GGATG 2 cut(s) 17, 406
Fsp4HI GCNGC 2 cut(s) 87, 473
FspBI CTAG 2 cut(s) 146, 332
GluI GCNGC 2 cut(s) 87, 473
HgaI GACGC 1 cut(s) 86
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 5 cut(s) 155, 277, 445, 472, 551
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HindIII AAGCTT 1 cut(s) 615
HinfI GANTC 2 cut(s) 9, 103
HpaI GTTAAC 1 cut(s) 601
HphI GGTGA 1 cut(s) 308
Hpy166II GTNNAC 1 cut(s) 601
Hpy188III TCNNGA 3 cut(s) 122, 239, 554
Hpy8I GTNNAC 1 cut(s) 601
HpyAV CCTTC 3 cut(s) 60, 383, 535
HpyCH4III ACNGT 6 cut(s) 143, 205, 260, 325, 364, 605
HpyCH4V TGCA 3 cut(s) 169, 279, 384
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 413, 422
HpyF3I CTNAG 3 cut(s) 116, 177, 233
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 5 cut(s) 155, 277, 445, 472, 551
Ksp22I TGATCA 1 cut(s) 129
KspAI GTTAAC 1 cut(s) 601
Kzo9I GATC 3 cut(s) 129, 456, 639
LmnI GCTCC 1 cut(s) 173
LpnPI CCDG 6 cut(s) 26, 135, 252, 357, 567, 580
Lsp1109I GCAGC 2 cut(s) 73, 459
LweI GCATC 3 cut(s) 37, 345, 364
MaeI CTAG 2 cut(s) 146, 332
MalI GATC 3 cut(s) 131, 458, 641
MboI GATC 3 cut(s) 129, 456, 639
MboII GAAGA 1 cut(s) 413
MfeI CAATTG 1 cut(s) 290
MluCI AATT 3 cut(s) 264, 290, 501
MlyI GAGTC 2 cut(s) 18, 97
MseI TTAA 5 cut(s) 33, 41, 600, 630, 652
MspA1I CMGCKG 1 cut(s) 16
MunI CAATTG 1 cut(s) 290
MwoI GCNNNNNNNGC 3 cut(s) 86, 413, 422
NdeII GATC 3 cut(s) 129, 456, 639
NlaIII CATG 5 cut(s) 155, 277, 445, 472, 551
NlaIV GGNNCC 1 cut(s) 187
NspI RCATGY 2 cut(s) 277, 472
PkrI GCNGC 2 cut(s) 88, 474
PleI GAGTC 2 cut(s) 17, 97
PpsI GAGTC 2 cut(s) 17, 97
PsiI TTATAA 1 cut(s) 26
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 2 cut(s) 69, 186
PvuII CAGCTG 1 cut(s) 16
RsaI GTAC 2 cut(s) 366, 571
RsaNI GTAC 2 cut(s) 365, 570
SaqAI TTAA 5 cut(s) 33, 41, 600, 630, 652
SatI GCNGC 2 cut(s) 87, 473
Sau3AI GATC 3 cut(s) 129, 456, 639
Sau96I GGNCC 2 cut(s) 69, 186
ScaI AGTACT 1 cut(s) 366
SchI GAGTC 2 cut(s) 18, 97
SfaNI GCATC 3 cut(s) 37, 345, 364
SinI GGWCC 2 cut(s) 69, 186
Sse9I AATT 3 cut(s) 264, 290, 501
SspMI CTAG 2 cut(s) 146, 332
StyI CCWWGG 2 cut(s) 72, 576
TaaI ACNGT 6 cut(s) 143, 205, 260, 325, 364, 605
TaqI TCGA 2 cut(s) 372, 465
TasI AATT 3 cut(s) 264, 290, 501
TatI WGTACW 2 cut(s) 364, 569
Tru1I TTAA 5 cut(s) 33, 41, 600, 630, 652
Tru9I TTAA 5 cut(s) 33, 41, 600, 630, 652
TscAI CASTG 1 cut(s) 208
TseI GCWGC 2 cut(s) 86, 472
TspDTI ATGAA 4 cut(s) 140, 333, 467, 627
TspRI CASTG 1 cut(s) 208
VpaK11BI GGWCC 2 cut(s) 69, 186
XceI RCATGY 2 cut(s) 277, 472
XspI CTAG 2 cut(s) 146, 332
ZrmI AGTACT 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.