Rroxscaffold_4G00276490

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006142
Physical Location & Seq
Forward (+)
63946 .. 68391
4446 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00276490.1

Sequence Viewer

Length: 3072 bp
ATGAATATGATGATGCGCCAGCGTTTGAAGATGCAAGCCAAGACTAGTCTACTTTTCCTGGCTCGATCTTTGAGTTCCTCCTCGTCCTACTCATTTGGCAAGCCATTCTTTGCTCCCCAAAACTACTACTCCATCCCACCATCCAAGACTCACCTCTATTCATCCTTCTTTTGCACCCTTATACACCTCTACTTGGCCTGCGGCAGATTCTCCGATGCTTCCCACGCCTTCTTCTCTATGCGAAGTCATGGCCTCGTTCCTGTTCTGCCTCTTTGGAATCGGCTTCTTCACCATTTCAGTGCCTCTGGTTTGTCTTCTCAGGTATCCCTTCTCTACTCTGAGATGCTCTCCTGTGGGGTTGTGCCTAGTGTTTTCACCCGCAACATATTGATTCACTCTTTCTGCAAAGTCGGAAACTTGAGTTTGGCACTGCATTTCTTAAGAAATACCCAAATTGATACTGTTTCCTATAACACTCTCATTTGGGGCTTCTCCCAACAAGGAATGCCTTATCAAGCTTTTGGATTTTTGTCTCAAATGCTGCGGAGGGCTATATTCATAGATTCTTATACTTGCAATTTACTAGTTAACGGGTTTTGCCGAATTGGCCTACTTGAGTATGCAGAATGGGTTATGGATAATTTGGTCAGTGGTGGAATTACTCAGGATGTTGTAGGTTTTAACACCTTGATTGCTGGATATTGCAAAGCTGGACAAGTTAGCCGTGCACTCCAGTTAATTGAGAGAATGGAGAGGGATGGTCTTCTTCCTGATACTCTTACTTATAATACTCTTGTTCATGGGTTTTGTCACGTGGGGGATTTTGCAAATGCCAAGAGTACAATACGTAACATTTTGAGATCTCAAGGAAACGAAGATGGCCCTCATAATGAGAGAGGCCATGATCAGAACTGGACTGGCAGTATGAATTTTAGACCAAATGTTATAACTCTCACCACGCTCATTAGCGCTTACGGTAAGCATCAAGGACTTGAAGAGGCTCTTTCTCTGTATGAGGAAATGCTTATGAATGGCATTTTCCCGGATGTTGTTACTTATAGCTCCATTATATATGGTCTTTGCAAGCATGGGAGGCTGTCAGAAGCCAAAGTGCTCTTAAGGGAGATAGAGAAAATGGGTGTAAATCCTAACAATGTCTCCTTCACTACCCTCGTGGATTCTTTGTTTAAGGCAGGCTCTTCTAGGGAAGCTCTCATCCTTCAAGGCCAAATGGTGGTTCGCGGCCTAGTTTTTGATTTAGTAATTTGCACTGCTTTAATGGCTGGACTGTTTAAAGTTGGAAAAGTCGGCGAGGCTAAGGACCTGTTTCAAACAATTTCAAAGCTCAGCTTAGTTCCAAACTGTATCACCTATTCTGCTTTGATAAATGGGCATTGCAATTCAGGAGACATGGGCGGTGCAGAAGCAATACTTAGAGAAATGGAAGATAAACATATTTTTCCAAATATTATCACTTATTCTTCTCTCATAAAGGGTTATATGAAGAAAGGAATGATTGGTGATGCTATTGGTTTATTGAGGAAGATGGTGCAAAAAAATGTATTGCCAAATGTTTTTGTGTATGCAATACTAATTGATGGGTGTTTTAAGGCAGGTAAACAAGAATTTGCTCTTGATCTCTACAATGAAATGAAAATTAGAGGATTGGAGGACAACAGTTTCATATTTGATGCTTTTGTGAACAACATGAAAAAGTGCAGAAGGATGGAACAAGCTGAGGGATTAATTATGGATATGACTTCTGAGGGCTTGTTTCTTGACCGTGTTAACTACACATCTCTGATGGATGGCTTCTTTAAAGTGGGAAAAGAGTCAATTGCTCTTAACTTGTTCGAAGAAATGGCGGAGAAAAATATAGGGTTTGATGTTGTTTCATACAATGTCTTGATAAATGGTCTGTTGCGGCTTGGGAAATATGAAGTCAAATCTGTCTATACTGGAATGAGAGAGTTGGGAGTGGCTCCCGACTGTGTCACGTACAATACCCTGATTATTGCATTCTGCAGAGAGGGTGACACTGAAAATGCTTTTGACCTTTGGAATGAGATGAAGGGTCAAGGGTTCATTTTAAATTCATTCACTTGTAACATTCTGATAGGAGGGCTTTGTGAAGAAGATAAGATTGAAAAAGCAATGGATGTCTTAAATGAAATGGTGGCTGTGGGCTCTCTCCCTACCTCAGTTACTCATAGGCTTCTGCTTGATGCTTCTTCAAAGAGTAGAAGAGCGGATCCAATTTTGCAAATGCATCAGAAGCTTGTAAGTATGGGGCTTGACCTCAATCATGATGTTTATAATAATCTCATCACTATCTTATGTAGGCTGGGAATGACCAGGAAGGCCACTACAGTATTAGAAGAGATGACTGGAAGAGGATTTTTAGCTGATACTATTACTTACAATGCCCTTATACGTGGATATGGTATGAGCAGCCATCTAAAAAGGGCTTTTGCTACACACTCAAAGATGCTGGCTGAGGGAGTTTCTCCAAATATTGAAACGTACAATATTCTTTTAGGGGTCCTTTCAGGTGCTGGTTTGATGACAAAGGCAGATGAATTATTTAGTGAAATGAAGAATAGAGGCTTTCTTCCTAATGCTTCCACATATGATATATTGGTTTCCGGCTATGGTAAGATTGGAAATAAGAAGGAAGCCATAAGGCGTTATTGTGAAATGGTAAGCAAAAGTTTTGTTCCCAATACTAGCACCTACAATGTGCTTATTAGTGATTTTGCTAAGGTGGGAAAGATGAGCCAAGCTAGGGAGCTTATGAATGAGATGCAGGCAAGGGGTGCCTGTCCTAATTCTTCAACATACAGCATACTAATCTGTGGCTGGTGTAAGCTATCAAAGCAGCCAGAGGTGGAGAAGAAGTTGCGAAAGTCATACCGAGCTGAGGCAAAGAGATTATTAGCCGAGATGAATGAGAAAGGGTATGTTCCAGGTGGAAGTACCCTTTCATCTATCAGTTCTACCTTTGCTAGACCAGGAAAGAAGGCTGATGCTCAGAGGCTATTAAAGGAACTGTACATCAGCAAGAACATATGA

Protein Analysis

1023

Amino Acids

114.02

Weight (kDa)

8.77

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 92 - 136 3.6e-07 PPR repeat family
PPR_2 PF13041 153 - 201 2.7e-12 PPR repeat family
PPR_3 PF13812 211 - 266 7e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 219 - 251 2.6e-09 PPR repeat
PPR_2 PF13041 223 - 271 7e-17 PPR repeat family
PPR PF01535 227 - 255 2e-07 PPR repeat
PPR_1 PF12854 253 - 278 2.3e-06 PPR repeat
PPR_3 PF13812 310 - 357 1.6e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 313 - 362 1.6e-15 PPR repeat family
PPR PF01535 316 - 346 5.2e-06 PPR repeat
PPR_1 PF12854 344 - 376 5.1e-12 PPR repeat
PPR_2 PF13041 348 - 397 8e-13 PPR repeat family
PPR PF01535 351 - 381 1.9e-06 PPR repeat
PPR_2 PF13041 419 - 467 1.6e-10 PPR repeat family
PPR_long PF17177 437 - 542 3e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 443 - 500 1.2e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 451 - 482 3.2e-09 PPR repeat
PPR PF01535 456 - 485 1e-05 PPR repeat
PPR_2 PF13041 456 - 500 7e-12 PPR repeat family
PPR_1 PF12854 485 - 517 6.2e-08 PPR repeat
PPR_2 PF13041 488 - 537 4.3e-14 PPR repeat family
PPR PF01535 491 - 521 2.1e-06 PPR repeat
PPR_2 PF13041 523 - 560 4.2e-07 PPR repeat family
PPR_3 PF13812 583 - 638 2.6e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 594 - 641 1.9e-10 PPR repeat family
PPR_long PF17177 613 - 760 1.8e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 617 - 674 1.3e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 629 - 676 1.6e-10 PPR repeat family
PPR_1 PF12854 659 - 691 2.6e-12 PPR repeat
PPR_2 PF13041 662 - 710 9.3e-17 PPR repeat family
PPR PF01535 665 - 695 8.1e-09 PPR repeat
PPR_1 PF12854 694 - 726 5.9e-06 PPR repeat
PPR_2 PF13041 698 - 743 1.9e-10 PPR repeat family
PPR_long PF17177 741 - 888 4.3e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 771 - 814 2.4e-09 PPR repeat family
TPR_24 PF23276 774 - 899 5.2e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 791 - 849 1.3e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 803 - 849 4.5e-11 PPR repeat family
PPR_long PF17177 816 - 950 3.9e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 837 - 885 2.1e-13 PPR repeat family
PPR_3 PF13812 861 - 918 4.8e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 875 - 921 1.1e-09 PPR repeat family
PPR_1 PF12854 904 - 936 5.1e-06 PPR repeat
PPR_2 PF13041 907 - 956 4.1e-15 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 786, 947, 2316
Acc36I ACCTGC 1 cut(s) 1604
AccB1I GGYRCC 1 cut(s) 2816
AccB7I CCANNNNNTGG 1 cut(s) 1234
AccBSI CCGCTC 1 cut(s) 2249
AccI GTMKAC 1 cut(s) 49
AccII CGCG 1 cut(s) 1242
AciI CCGC 8 cut(s) 201, 379, 544, 1242, 1416, 1865, 1924, 2249
AclWI GGATC 2 cut(s) 2246, 2259
AcsI RAATTY 3 cut(s) 928, 1625, 2092
AcvI CACGTG 1 cut(s) 814
AfaI GTAC 5 cut(s) 841, 2000, 2525, 2977, 3053
AfeI AGCGCT 1 cut(s) 970
AfiI CCNNNNNNNGG 4 cut(s) 193, 1234, 2785, 2920
AflII CTTAAG 2 cut(s) 439, 1117
AgsI TTSAA 9 cut(s) 28, 995, 1223, 1331, 1341, 2147, 2235, 2519, 2835
AhlI ACTAGT 2 cut(s) 44, 583
AjnI CCWGG 4 cut(s) 57, 2354, 2965, 3010
AjuI GAANNNNNNNTTGG 2 cut(s) 1221, 1253
Alw21I GWGCWC 2 cut(s) 730, 1116
Alw26I GTCTC 3 cut(s) 537, 1162, 1401
Alw44I GTGCAC 1 cut(s) 726
AlwI GGATC 2 cut(s) 2246, 2259
AlwNI CAGNNNCTG 1 cut(s) 2555
Aor51HI AGCGCT 1 cut(s) 970
AoxI GGCC 8 cut(s) 195, 250, 607, 880, 898, 1225, 1243, 2361
ApaLI GTGCAC 1 cut(s) 726
ApeKI GCWGC 3 cut(s) 541, 2451, 2878
ApoI RAATTY 3 cut(s) 928, 1625, 2092
AseI ATTAAT 1 cut(s) 1745
AspLEI GCGC 2 cut(s) 18, 971
AspS9I GGNCC 3 cut(s) 881, 1321, 2542
AsuC2I CCSGG 1 cut(s) 1043
AsuHPI GGTGA 7 cut(s) 143, 281, 367, 946, 1360, 1532, 2045
AsuII TTCGAA 1 cut(s) 1854
AvaII GGWCC 2 cut(s) 1321, 2542
BaeGI GKGCMC 1 cut(s) 730
BamHI GGATCC 1 cut(s) 2251
BanI GGYRCC 1 cut(s) 2816
BanII GRGCYC 1 cut(s) 2189
BauI CACGAG 1 cut(s) 1172
BbrPI CACGTG 1 cut(s) 814
BbsI GAAGAC 2 cut(s) 306, 755
Bbv12I GWGCWC 2 cut(s) 730, 1116
BbvCI CCTCAGC 3 cut(s) 1737, 2496, 2919
BbvI GCAGC 3 cut(s) 528, 2463, 2890
BceAI ACGGC 1 cut(s) 708
BciT130I CCWGG 4 cut(s) 59, 2356, 2967, 3012
BciVI GTATCC 1 cut(s) 334
BclI TGATCA 1 cut(s) 904
BcnI CCSGG 1 cut(s) 1043
BcoDI GTCTC 3 cut(s) 537, 1162, 1401
BcuI ACTAGT 2 cut(s) 44, 583
BfaI CTAG 8 cut(s) 45, 366, 584, 1203, 1247, 2727, 2784, 3006
BfmI CTRYAG 2 cut(s) 2023, 2367
BfoI RGCGCY 1 cut(s) 972
BfrI CTTAAG 2 cut(s) 439, 1117
BfuAI ACCTGC 1 cut(s) 1604
BfuI GTATCC 1 cut(s) 334
BglI GCCNNNNNGGC 1 cut(s) 606
BglII AGATCT 1 cut(s) 860
BisI GCNGC 6 cut(s) 202, 542, 1243, 1925, 2452, 2879
BlpI GCTNAGC 1 cut(s) 1346
BlsI GCNGC 6 cut(s) 203, 543, 1244, 1926, 2453, 2880
Bme1390I CCNGG 5 cut(s) 59, 1043, 2356, 2967, 3012
Bme18I GGWCC 2 cut(s) 1321, 2542
BmgT120I GGNCC 3 cut(s) 881, 1321, 2542
BmiI GGNNCC 4 cut(s) 1983, 2253, 2543, 2818
BmrFI CCNGG 5 cut(s) 59, 1043, 2356, 2967, 3012
BpiI GAAGAC 2 cut(s) 306, 755
BplI GAGNNNNNCTC 2 cut(s) 332, 364
BpmI CTGGAG 1 cut(s) 716
Bpu10I CCTNAGC 5 cut(s) 1317, 1737, 2496, 2760, 2919
Bpu1102I GCTNAGC 1 cut(s) 1346
Bpu14I TTCGAA 1 cut(s) 1854
BpuEI CTTGAG 3 cut(s) 439, 635, 849
BpuMI CCSGG 1 cut(s) 1043
BsaAI YACGTR 4 cut(s) 814, 848, 1998, 2435
BsaXI ACNNNNNCTCC 6 cut(s) 113, 143, 1142, 1172, 2882, 2912
Bsc4I CCNNNNNNNGG 4 cut(s) 193, 1234, 2785, 2920
Bse1I ACTGG 5 cut(s) 733, 917, 922, 1963, 2392
Bse3DI GCAATG 2 cut(s) 1393, 2160
BseBI CCWGG 4 cut(s) 59, 2356, 2967, 3012
BseLI CCNNNNNNNGG 4 cut(s) 193, 1234, 2785, 2920
BseMI GCAATG 2 cut(s) 1393, 2160
BseNI ACTGG 5 cut(s) 733, 917, 922, 1963, 2392
BseRI GAGGAG 1 cut(s) 70
BseSI GKGCMC 1 cut(s) 730
BseXI GCAGC 3 cut(s) 528, 2463, 2890
BseYI CCCAGC 1 cut(s) 2344
BsgI GTGCAG 2 cut(s) 1440, 1738
Bsh1236I CGCG 1 cut(s) 1242
BshFI GGCC 8 cut(s) 197, 252, 609, 882, 900, 1227, 1245, 2363
BshNI GGYRCC 1 cut(s) 2816
BsiHKAI GWGCWC 2 cut(s) 730, 1116
BsiSI CCGG 2 cut(s) 1043, 2646
BslI CCNNNNNNNGG 4 cut(s) 193, 1234, 2785, 2920
BsmAI GTCTC 3 cut(s) 537, 1162, 1401
BsmI GAATGC 2 cut(s) 510, 2018
BsnI GGCC 8 cut(s) 197, 252, 609, 882, 900, 1227, 1245, 2363
Bsp119I TTCGAA 1 cut(s) 1854
Bsp1286I GDGCHC 3 cut(s) 730, 1116, 2189
Bsp1407I TGTACA 1 cut(s) 3051
Bsp143I GATC 5 cut(s) 65, 860, 904, 1636, 2251
Bsp1720I GCTNAGC 1 cut(s) 1346
BspACI CCGC 8 cut(s) 201, 379, 544, 1242, 1416, 1865, 1924, 2249
BspANI GGCC 8 cut(s) 197, 252, 609, 882, 900, 1227, 1245, 2363
BspFNI CGCG 1 cut(s) 1242
BspHI TCATGA 1 cut(s) 2305
BspLI GGNNCC 4 cut(s) 1983, 2253, 2543, 2818
BspMAI CTGCAG 1 cut(s) 2027
BspMI ACCTGC 1 cut(s) 1604
BspPI GGATC 2 cut(s) 2246, 2259
BspQI GCTCTTC 2 cut(s) 1204, 2239
BspT104I TTCGAA 1 cut(s) 1854
BspT107I GGYRCC 1 cut(s) 2816
BspTI CTTAAG 2 cut(s) 439, 1117
BsrBI CCGCTC 1 cut(s) 2249
BsrDI GCAATG 2 cut(s) 1393, 2160
BsrGI TGTACA 1 cut(s) 3051
BsrI ACTGG 5 cut(s) 733, 917, 922, 1963, 2392
BssMI GATC 5 cut(s) 65, 860, 904, 1636, 2251
BssSI CACGAG 1 cut(s) 1172
Bst2BI CACGAG 1 cut(s) 1172
Bst2UI CCWGG 4 cut(s) 59, 2356, 2967, 3012
Bst4CI ACNGT 9 cut(s) 463, 977, 1290, 1364, 1679, 1784, 1991, 2371, 3051
Bst6I CTCTTC 5 cut(s) 990, 1204, 2239, 2373, 2386
BstAFI CTTAAG 2 cut(s) 439, 1117
BstAPI GCANNNNNTGC 1 cut(s) 2816
BstAUI TGTACA 1 cut(s) 3051
BstBAI YACGTR 4 cut(s) 814, 848, 1998, 2435
BstBI TTCGAA 1 cut(s) 1854
BstC8I GCNNGC 8 cut(s) 20, 36, 101, 199, 1085, 1195, 2493, 2808
BstFNI CGCG 1 cut(s) 1242
BstH2I RGCGCY 1 cut(s) 972
BstHHI GCGC 2 cut(s) 18, 971
BstKTI GATC 5 cut(s) 68, 863, 907, 1639, 2254
BstMAI GTCTC 3 cut(s) 537, 1162, 1401
BstMBI GATC 5 cut(s) 65, 860, 904, 1636, 2251
BstMWI GCNNNNNNNGC 7 cut(s) 224, 606, 1093, 1280, 2275, 2816, 2875
BstNI CCWGG 4 cut(s) 59, 2356, 2967, 3012
BstSCI CCNGG 5 cut(s) 57, 1041, 2354, 2965, 3010
BstSFI CTRYAG 2 cut(s) 2023, 2367
BstSLI GKGCMC 1 cut(s) 730
BstSNI TACGTA 1 cut(s) 848
BstUI CGCG 1 cut(s) 1242
BstV1I GCAGC 3 cut(s) 528, 2463, 2890
BstV2I GAAGAC 2 cut(s) 306, 755
BstX2I RGATCY 2 cut(s) 860, 2251
BstYI RGATCY 2 cut(s) 860, 2251
BsuI GTATCC 1 cut(s) 334
BsuRI GGCC 8 cut(s) 197, 252, 609, 882, 900, 1227, 1245, 2363
BtsI GCAGTG 2 cut(s) 428, 1269
BtsIMutI CAGTG 5 cut(s) 304, 428, 655, 1269, 2037
BveI ACCTGC 1 cut(s) 1604
Cac8I GCNNGC 8 cut(s) 20, 36, 101, 199, 1085, 1195, 2493, 2808
CaiI CAGNNNCTG 1 cut(s) 2555
CciI TCATGA 1 cut(s) 2305
CfoI GCGC 2 cut(s) 18, 971
Cfr13I GGNCC 3 cut(s) 881, 1321, 2542
Csp6I GTAC 5 cut(s) 840, 1999, 2524, 2976, 3052
CviAII CATG 7 cut(s) 248, 800, 902, 1088, 1411, 1708, 2306
CviQI GTAC 5 cut(s) 840, 1999, 2524, 2976, 3052
DpnI GATC 5 cut(s) 67, 862, 906, 1638, 2253
DpnII GATC 5 cut(s) 65, 860, 904, 1636, 2251
DraI TTTAAA 3 cut(s) 1294, 1819, 2091
Eam1104I CTCTTC 5 cut(s) 990, 1204, 2239, 2373, 2386
EarI CTCTTC 5 cut(s) 990, 1204, 2239, 2373, 2386
EciI GGCGGA 1 cut(s) 1880
Eco105I TACGTA 1 cut(s) 848
Eco24I GRGCYC 1 cut(s) 2189
Eco47I GGWCC 2 cut(s) 1321, 2542
Eco47III AGCGCT 1 cut(s) 970
Eco72I CACGTG 1 cut(s) 814
EcoO109I RGGNCCY 2 cut(s) 1321, 2542
EcoRII CCWGG 4 cut(s) 57, 2354, 2965, 3010
EcoT22I ATGCAT 1 cut(s) 2271
EcoT38I GRGCYC 1 cut(s) 2189
FaeI CATG 7 cut(s) 251, 803, 905, 1091, 1414, 1711, 2309
FalI AAGNNNNNCTT 8 cut(s) 92, 124, 987, 1019, 1334, 1366, 1416, 1448
FatI CATG 7 cut(s) 247, 799, 901, 1087, 1410, 1707, 2305
FauI CCCGC 1 cut(s) 386
FauNDI CATATG 2 cut(s) 2629, 3068
FbaI TGATCA 1 cut(s) 904
FblI GTMKAC 1 cut(s) 49
Fnu4HI GCNGC 6 cut(s) 202, 542, 1243, 1925, 2452, 2879
FriOI GRGCYC 1 cut(s) 2189
Fsp4HI GCNGC 6 cut(s) 202, 542, 1243, 1925, 2452, 2879
FspBI CTAG 8 cut(s) 45, 366, 584, 1203, 1247, 2727, 2784, 3006
GlaI GCGC 2 cut(s) 17, 970
GluI GCNGC 6 cut(s) 202, 542, 1243, 1925, 2452, 2879
GsaI CCCAGC 1 cut(s) 2348
GsuI CTGGAG 1 cut(s) 716
HaeII RGCGCY 1 cut(s) 972
HaeIII GGCC 8 cut(s) 197, 252, 609, 882, 900, 1227, 1245, 2363
HapII CCGG 2 cut(s) 1043, 2646
HhaI GCGC 2 cut(s) 18, 971
Hin1II CATG 7 cut(s) 251, 803, 905, 1091, 1414, 1711, 2309
Hin6I GCGC 2 cut(s) 16, 969
HinP1I GCGC 2 cut(s) 16, 969
HincII GTYRAC 2 cut(s) 589, 1789
HindII GTYRAC 2 cut(s) 589, 1789
HindIII AAGCTT 2 cut(s) 516, 2276
HinfI GANTC 7 cut(s) 148, 207, 277, 391, 563, 1178, 1832
HpaI GTTAAC 2 cut(s) 589, 1789
HpaII CCGG 2 cut(s) 1043, 2646
HphI GGTGA 7 cut(s) 143, 281, 367, 946, 1360, 1532, 2045
Hpy166II GTNNAC 6 cut(s) 50, 589, 728, 1619, 1702, 1789
Hpy188III TCNNGA 8 cut(s) 665, 770, 1404, 1634, 1778, 1906, 1985, 2306
Hpy8I GTNNAC 6 cut(s) 50, 589, 728, 1619, 1702, 1789
HpyCH4III ACNGT 9 cut(s) 463, 977, 1290, 1364, 1679, 1784, 1991, 2371, 3051
HpyCH4IV ACGT 5 cut(s) 813, 847, 1997, 2434, 2522
HpyF10VI GCNNNNNNNGC 7 cut(s) 224, 606, 1093, 1280, 2275, 2816, 2875
HpySE526I ACGT 5 cut(s) 813, 847, 1997, 2434, 2522
Hsp92II CATG 7 cut(s) 251, 803, 905, 1091, 1414, 1711, 2309
HspAI GCGC 2 cut(s) 16, 969
Ksp22I TGATCA 1 cut(s) 904
KspAI GTTAAC 2 cut(s) 589, 1789
Kzo9I GATC 5 cut(s) 65, 860, 904, 1636, 2251
LguI GCTCTTC 2 cut(s) 1204, 2239
LmnI GCTCC 4 cut(s) 118, 1067, 1987, 2788
Lsp1109I GCAGC 3 cut(s) 528, 2463, 2890
MaeI CTAG 8 cut(s) 45, 366, 584, 1203, 1247, 2727, 2784, 3006
MaeII ACGT 5 cut(s) 813, 847, 1997, 2434, 2522
MaeIII GTNAC 7 cut(s) 809, 848, 1051, 1993, 2033, 2105, 2203
MalI GATC 5 cut(s) 67, 862, 906, 1638, 2253
MbiI CCGCTC 1 cut(s) 2249
MboI GATC 5 cut(s) 65, 860, 904, 1636, 2251
MfeI CAATTG 1 cut(s) 1836
MflI RGATCY 2 cut(s) 860, 2251
MhlI GDGCHC 3 cut(s) 730, 1116, 2189
MlyI GAGTC 2 cut(s) 142, 1841
MmeI TCCRAC 2 cut(s) 391, 1279
Mph1103I ATGCAT 1 cut(s) 2271
MslI CAYNNNNRTG 1 cut(s) 297
MspCI CTTAAG 2 cut(s) 439, 1117
MspI CCGG 2 cut(s) 1043, 2646
MspR9I CCNGG 5 cut(s) 59, 1043, 2356, 2967, 3012
MunI CAATTG 1 cut(s) 1836
Mva1269I GAATGC 2 cut(s) 510, 2018
MvaI CCWGG 4 cut(s) 59, 2356, 2967, 3012
MvnI CGCG 1 cut(s) 1242
MwoI GCNNNNNNNGC 7 cut(s) 224, 606, 1093, 1280, 2275, 2816, 2875
NciI CCSGG 1 cut(s) 1043
NdeI CATATG 2 cut(s) 2629, 3068
NdeII GATC 5 cut(s) 65, 860, 904, 1636, 2251
NlaIII CATG 7 cut(s) 251, 803, 905, 1091, 1414, 1711, 2309
NlaIV GGNNCC 4 cut(s) 1983, 2253, 2543, 2818
NmeAIII GCCGAG 1 cut(s) 2965
NmuCI GTSAC 3 cut(s) 809, 1993, 2033
NsiI ATGCAT 1 cut(s) 2271
NspV TTCGAA 1 cut(s) 1854
PagI TCATGA 1 cut(s) 2305
PciSI GCTCTTC 2 cut(s) 1204, 2239
PctI GAATGC 2 cut(s) 510, 2018
PfeI GAWTC 5 cut(s) 207, 277, 391, 563, 1178
PflFI GACNNNGTC 1 cut(s) 1991
PflMI CCANNNNNTGG 1 cut(s) 1234
PfoI TCCNGGA 1 cut(s) 1041
PkrI GCNGC 6 cut(s) 203, 543, 1244, 1926, 2453, 2880
PleI GAGTC 2 cut(s) 142, 1840
PmaCI CACGTG 1 cut(s) 814
PmlI CACGTG 1 cut(s) 814
PpsI GAGTC 2 cut(s) 142, 1840
Ppu21I YACGTR 4 cut(s) 814, 848, 1998, 2435
PpuMI RGGWCCY 2 cut(s) 1321, 2542
PshBI ATTAAT 1 cut(s) 1745
PsiI TTATAA 3 cut(s) 786, 947, 2316
Psp5II RGGWCCY 2 cut(s) 1321, 2542
Psp6I CCWGG 4 cut(s) 57, 2354, 2965, 3010
PspCI CACGTG 1 cut(s) 814
PspFI CCCAGC 1 cut(s) 2344
PspGI CCWGG 4 cut(s) 57, 2354, 2965, 3010
PspN4I GGNNCC 4 cut(s) 1983, 2253, 2543, 2818
PspPI GGNCC 3 cut(s) 881, 1321, 2542
PspPPI RGGWCCY 2 cut(s) 1321, 2542
PstI CTGCAG 1 cut(s) 2027
PstNI CAGNNNCTG 1 cut(s) 2555
PsuI RGATCY 2 cut(s) 860, 2251
PsyI GACNNNGTC 1 cut(s) 1991
RsaI GTAC 5 cut(s) 841, 2000, 2525, 2977, 3053
RsaNI GTAC 5 cut(s) 840, 1999, 2524, 2976, 3052
RseI CAYNNNNRTG 1 cut(s) 297
SapI GCTCTTC 2 cut(s) 1204, 2239
SatI GCNGC 6 cut(s) 202, 542, 1243, 1925, 2452, 2879
Sau3AI GATC 5 cut(s) 65, 860, 904, 1636, 2251
Sau96I GGNCC 3 cut(s) 881, 1321, 2542
SchI GAGTC 2 cut(s) 142, 1841
ScrFI CCNGG 5 cut(s) 59, 1043, 2356, 2967, 3012
SduI GDGCHC 3 cut(s) 730, 1116, 2189
SfcI CTRYAG 2 cut(s) 2023, 2367
SfuI TTCGAA 1 cut(s) 1854
SinI GGWCC 2 cut(s) 1321, 2542
SmiMI CAYNNNNRTG 1 cut(s) 297
SmlI CTYRAG 5 cut(s) 418, 439, 614, 864, 1117
SmoI CTYRAG 5 cut(s) 418, 439, 614, 864, 1117
SnaBI TACGTA 1 cut(s) 848
SpeI ACTAGT 2 cut(s) 44, 583
SsiI CCGC 8 cut(s) 201, 379, 544, 1242, 1416, 1865, 1924, 2249
SspI AATATT 3 cut(s) 1468, 2515, 2530
SspMI CTAG 8 cut(s) 45, 366, 584, 1203, 1247, 2727, 2784, 3006
StyD4I CCNGG 5 cut(s) 57, 1041, 2354, 2965, 3010
TaaI ACNGT 9 cut(s) 463, 977, 1290, 1364, 1679, 1784, 1991, 2371, 3051
TaiI ACGT 5 cut(s) 816, 850, 2000, 2437, 2525
TaqI TCGA 2 cut(s) 64, 1854
TatI WGTACW 2 cut(s) 839, 3051
TauI GCSGC 3 cut(s) 204, 1245, 1927
TfiI GAWTC 5 cut(s) 207, 277, 391, 563, 1178
TscAI CASTG 5 cut(s) 304, 435, 655, 1276, 2044
TseFI GTSAC 3 cut(s) 809, 1993, 2033
TseI GCWGC 3 cut(s) 541, 2451, 2878
Tsp45I GTSAC 3 cut(s) 809, 1993, 2033
TspRI CASTG 5 cut(s) 304, 435, 655, 1276, 2044
Tth111I GACNNNGTC 1 cut(s) 1991
Van91I CCANNNNNTGG 1 cut(s) 1234
Vha464I CTTAAG 2 cut(s) 439, 1117
VneI GTGCAC 1 cut(s) 726
VpaK11BI GGWCC 2 cut(s) 1321, 2542
VspI ATTAAT 1 cut(s) 1745
XapI RAATTY 3 cut(s) 928, 1625, 2092
XmiI GTMKAC 1 cut(s) 49
XspI CTAG 8 cut(s) 45, 366, 584, 1203, 1247, 2727, 2784, 3006
Zsp2I ATGCAT 1 cut(s) 2271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.