MD11G1161100.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0006142
Physical Location & Seq
Reverse (-)
15925750 .. 15927868
2119 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1161100.v1.1.491

Sequence Viewer

Length: 1695 bp
ATGGCATTTGCAAGTATGGGAGGCTATCAGAAGCCAAAGTGCTTTTTAAGGGAGATGGAGGAAATGGGTGTGGATCCTAATCATATCTCCTATACTACCCTTGTGGATTTCTTGTTTAAGGCAGGCTCTTCCGTGGAAGCTCTTGCCCTTCAAAGCCAAATGGTTGTTGGGAAAGCTGTTGATGCTAAAGACCTGTTTCGAACAATTTCAAAGCTTGGCCTTGTTCCAAACTCCATCACCTACTCTGCGTTCATACATGGACTTTGCAATTTAGGAGACATGAATGATGCAGAATCTGTACTTAAAGAAATGGAGAAGCGCCACATTCTCCCAAATATTATTACTTATTCTTCAATGATAAATGGCTTCGTGAAGGAAGGAAAGCTTGGGGAGGCCATGAGTTTATTAAGGGAGATGGTGCAAAAGAATATCCTGCCAAATGCTTTTGTGTATGCAACACTAATTGACGGGTGCTTTAAGGCAGGTAAACAAGAGCTCGCTCTTGATCTTTACAATGAAATGAAAATGGGAGGAGTGGAGGATAACAATTTTGTACTTGATACTTTTTTGAACAACATGAAAAGGCGCAGAAGGATGGAGGAAACTGAAGGATTAATTATGGATATGACTTCTCGGGGTTTGTTTCTTGATCGCGTTAACTACACATCTCTAATGGATGGTTACTTTAAAGAGCGAAAGGAGTCAGTTGCTCTGAACTTGGCCCAAGAGATGATGGAGAAAAATATAGGGTTTGATGTTGTTGCATACAATGTCTTAATGAATGGTCTATTGAGGCTTGGGAAGTATGAAGCAATATCTGTTTGTACTGGAATGAAAAAGTTGGGTTTGGCTCTTGACTATGCCACATACAATACCATGATTAATGCATTCTGTAGAGAGGGTGACGCTGAAAATGCTTTTAAACTCTGGCATGAGATGAAGTGTCAAGGGTTAATTCCAAACTCAAACACTTGTAACATTCTGATCCGAGGACTTTGCGATGCAAATGAGATTGAAAAAGCCATAGATGTCTTGAATGGAATGTTGTCTGTTGGTTCTCTCCCTACCTCATTTATTCATAGAATTCTGCTTGATGCATCTTCAAAGAGTAGAAGAGCTGATTCAATTTTGCAGATGCATCATAAGCTTGTATGTATGGGGCTTAACCTCAATCGGGATGTTTATAATAATCTCATCATTGTCTTGTGTAGGCTAGGTATGACCATGAAAGCCACTTTAGTGTTGAAAGAGATGATTGGAGGAGGATTTTTAGCAGATATTGATACTTACAATGCCTTTATATGTGGATATTGTGGACTTTCAAGTGCTGGTCTGATGACAGATGCGGAGGAGTTATTTGGTCAAATGAAGAACAGAGGTTTTGTTCCTAATGCTTCTACATATGATACTTTGGTTTCTGGTCATGGTAAGAAGGGAAATAAAAAGGAAGCTATAAGACTTTATTGTGAAATGGTAAGCAAGGGTTTTGTTCCCAAAACTAGCACTTACAATATCCTTATTAGTGATTTTGCTAAGGCGGGAAAGATGGGCCAAGCTAGGGAGCTTATGAATGAGATGCAGACAAGAGGGACCTCTCCTAACTCTTCAACTTACAACATACTAATTTGCGGCTGGTGTTGGCTATCAAAGCAGCCAGAGCTGGAGAGAAGTTTGAAAAAAGTCATATCGGGCTGA

Protein Analysis

565

Amino Acids

62.85

Weight (kDa)

8.05

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 62 - 174 4.6e-07 Fungal tetratrico peptide repeats
PPR_long PF17177 63 - 165 7.7e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 65 - 125 9.1e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 73 - 105 1.3e-10 PPR repeat
PPR_2 PF13041 76 - 125 4.9e-16 PPR repeat family
PPR PF01535 79 - 108 1.7e-06 PPR repeat
PPR_1 PF12854 108 - 140 6.7e-10 PPR repeat
PPR_2 PF13041 111 - 160 5.7e-15 PPR repeat family
PPR PF01535 114 - 144 7.8e-09 PPR repeat
PPR_3 PF13812 134 - 180 7.6e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 142 - 175 4.7e-06 PPR repeat
PPR_2 PF13041 146 - 184 1e-07 PPR repeat family
PPR_3 PF13812 206 - 261 1.6e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 217 - 264 1.7e-08 PPR repeat family
PPR_long PF17177 235 - 383 3e-09 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 252 - 299 2.3e-10 PPR repeat family
TPR_24 PF23276 271 - 351 9.7e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 275 - 330 2.9e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 282 - 314 1.2e-10 PPR repeat
PPR_2 PF13041 287 - 334 1.2e-18 PPR repeat family
PPR PF01535 289 - 317 1.7e-09 PPR repeat
PPR_1 PF12854 317 - 348 5.2e-06 PPR repeat
PPR_2 PF13041 320 - 366 1.8e-07 PPR repeat family
PPR_2 PF13041 394 - 438 5.6e-06 PPR repeat family
PPR_2 PF13041 426 - 475 9.2e-11 PPR repeat family
PPR_long PF17177 446 - 541 4.1e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 452 - 508 8e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 459 - 492 8.3e-07 PPR repeat
PPR_2 PF13041 463 - 512 3.4e-12 PPR repeat family
PPR PF01535 466 - 496 5.3e-06 PPR repeat
PPR_3 PF13812 487 - 542 3.4e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 494 - 527 5.3e-07 PPR repeat
PPR_2 PF13041 498 - 546 2.7e-15 PPR repeat family
PPR PF01535 501 - 530 5.8e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1185
Acc36I ACCTGC 1 cut(s) 473
AccII CGCG 1 cut(s) 654
AciI CCGC 3 cut(s) 1346, 1538, 1629
AclWI GGATC 3 cut(s) 68, 81, 979
AcsI RAATTY 1 cut(s) 1083
AcuI CTGAAG 1 cut(s) 627
AfaI GTAC 3 cut(s) 300, 555, 826
AfiI CCNNNNNNNGG 2 cut(s) 1174, 1558
AjuI GAANNNNNNNTTGG 2 cut(s) 369, 401
AleI CACNNNNGTG 1 cut(s) 1238
Alw21I GWGCWC 1 cut(s) 498
Alw26I GTCTC 1 cut(s) 270
AlwI GGATC 3 cut(s) 68, 81, 979
AlwNI CAGNNNCTG 1 cut(s) 296
Ama87I CYCGRG 1 cut(s) 633
AoxI GGCC 4 cut(s) 217, 393, 720, 1549
ApeKI GCWGC 1 cut(s) 1651
ApoI RAATTY 1 cut(s) 1083
AseI ATTAAT 2 cut(s) 614, 882
Asp700I GAANNNNTTC 1 cut(s) 205
AspLEI GCGC 2 cut(s) 321, 588
AspS9I GGNCC 3 cut(s) 721, 1549, 1590
AsuHPI GGTGA 2 cut(s) 229, 914
AsuII TTCGAA 1 cut(s) 199
AvaI CYCGRG 1 cut(s) 633
AvaII GGWCC 1 cut(s) 1590
BaeI ACNNNNGTAYC 2 cut(s) 1398, 1431
BamHI GGATCC 1 cut(s) 73
BanII GRGCYC 1 cut(s) 498
Bbv12I GWGCWC 1 cut(s) 498
BbvI GCAGC 1 cut(s) 1663
BccI CCATC 7 cut(s) 49, 242, 409, 589, 671, 727, 1540
BcgI CGANNNNNNTGC 2 cut(s) 978, 1012
BcoDI GTCTC 1 cut(s) 270
BfaI CTAG 3 cut(s) 1214, 1500, 1557
BfmI CTRYAG 1 cut(s) 892
BfoI RGCGCY 1 cut(s) 322
BfuAI ACCTGC 1 cut(s) 473
BisI GCNGC 2 cut(s) 1630, 1652
BlsI GCNGC 2 cut(s) 1631, 1653
Bme18I GGWCC 1 cut(s) 1590
BmeT110I CYCGRG 1 cut(s) 633
BmgT120I GGNCC 3 cut(s) 721, 1549, 1590
BmiI GGNNCC 2 cut(s) 75, 1591
BmsI GCATC 9 cut(s) 172, 277, 991, 1084, 1106, 1125, 1147, 1333, 1566
BpmI CTGGAG 1 cut(s) 1682
Bpu10I CCTNAGC 1 cut(s) 1533
Bpu14I TTCGAA 1 cut(s) 199
BsaBI GATNNNNATC 1 cut(s) 78
BsaJI CCNNGG 2 cut(s) 132, 988
Bsc4I CCNNNNNNNGG 2 cut(s) 1174, 1558
Bse1I ACTGG 1 cut(s) 832
Bse8I GATNNNNATC 1 cut(s) 78
BseDI CCNNGG 2 cut(s) 132, 988
BseGI GGATG 3 cut(s) 600, 682, 1183
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 2 cut(s) 1174, 1558
BseNI ACTGG 1 cut(s) 832
BseRI GAGGAG 3 cut(s) 546, 1275, 1364
BseXI GCAGC 1 cut(s) 1663
Bsh1236I CGCG 1 cut(s) 654
BshFI GGCC 4 cut(s) 219, 395, 722, 1551
BsiHKAI GWGCWC 1 cut(s) 498
BsiHKCI CYCGRG 1 cut(s) 633
BslFI GGGAC 1 cut(s) 1603
BslI CCNNNNNNNGG 2 cut(s) 1174, 1558
BsmAI GTCTC 1 cut(s) 270
BsmFI GGGAC 1 cut(s) 1603
BsmI GAATGC 1 cut(s) 887
BsnI GGCC 4 cut(s) 219, 395, 722, 1551
BsoBI CYCGRG 1 cut(s) 633
Bsp119I TTCGAA 1 cut(s) 199
Bsp1286I GDGCHC 1 cut(s) 498
Bsp143I GATC 4 cut(s) 73, 505, 649, 984
BspACI CCGC 3 cut(s) 1346, 1538, 1629
BspANI GGCC 4 cut(s) 219, 395, 722, 1551
BspFNI CGCG 1 cut(s) 654
BspLI GGNNCC 2 cut(s) 75, 1591
BspMI ACCTGC 1 cut(s) 473
BspPI GGATC 3 cut(s) 68, 81, 979
BspQI GCTCTTC 2 cut(s) 133, 1108
BspT104I TTCGAA 1 cut(s) 199
BsrI ACTGG 1 cut(s) 832
BssECI CCNNGG 2 cut(s) 132, 988
BssMI GATC 4 cut(s) 73, 505, 649, 984
Bst6I CTCTTC 3 cut(s) 133, 1108, 1609
BstBI TTCGAA 1 cut(s) 199
BstC8I GCNNGC 2 cut(s) 124, 498
BstDEI CTNAG 1 cut(s) 1533
BstDSI CCRYGG 1 cut(s) 132
BstF5I GGATG 3 cut(s) 600, 682, 1183
BstFNI CGCG 1 cut(s) 654
BstH2I RGCGCY 1 cut(s) 322
BstHHI GCGC 2 cut(s) 321, 588
BstKTI GATC 4 cut(s) 76, 508, 652, 987
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 4 cut(s) 73, 505, 649, 984
BstMWI GCNNNNNNNGC 5 cut(s) 182, 914, 1144, 1648, 1657
BstSFI CTRYAG 1 cut(s) 892
BstUI CGCG 1 cut(s) 654
BstV1I GCAGC 1 cut(s) 1663
BstX2I RGATCY 1 cut(s) 73
BstYI RGATCY 1 cut(s) 73
BsuRI GGCC 4 cut(s) 219, 395, 722, 1551
BtgI CCRYGG 1 cut(s) 132
BtgZI GCGATG 1 cut(s) 1014
BtsCI GGATG 3 cut(s) 600, 682, 1183
BveI ACCTGC 1 cut(s) 473
Cac8I GCNNGC 2 cut(s) 124, 498
CaiI CAGNNNCTG 1 cut(s) 296
CfoI GCGC 2 cut(s) 321, 588
Cfr13I GGNCC 3 cut(s) 721, 1549, 1590
CseI GACGC 1 cut(s) 914
Csp6I GTAC 3 cut(s) 299, 554, 825
CviAII CATG 8 cut(s) 257, 280, 397, 577, 877, 932, 1225, 1424
CviQI GTAC 3 cut(s) 299, 554, 825
DdeI CTNAG 1 cut(s) 1533
DpnI GATC 4 cut(s) 75, 507, 651, 986
DpnII GATC 4 cut(s) 73, 505, 649, 984
DraI TTTAAA 2 cut(s) 688, 922
Eam1104I CTCTTC 3 cut(s) 133, 1108, 1609
EarI CTCTTC 3 cut(s) 133, 1108, 1609
Ecl136II GAGCTC 1 cut(s) 496
Eco24I GRGCYC 1 cut(s) 498
Eco47I GGWCC 1 cut(s) 1590
Eco53kI GAGCTC 1 cut(s) 496
Eco57I CTGAAG 1 cut(s) 627
Eco88I CYCGRG 1 cut(s) 633
EcoICRI GAGCTC 1 cut(s) 496
EcoO109I RGGNCCY 1 cut(s) 1590
EcoRI GAATTC 1 cut(s) 1083
EcoT22I ATGCAT 3 cut(s) 889, 1099, 1140
EcoT38I GRGCYC 1 cut(s) 498
FaeI CATG 8 cut(s) 260, 283, 400, 580, 880, 935, 1228, 1427
FalI AAGNNNNNCTT 2 cut(s) 369, 401
FaqI GGGAC 1 cut(s) 1603
FatI CATG 8 cut(s) 256, 279, 396, 576, 876, 931, 1224, 1423
FauI CCCGC 1 cut(s) 1531
FauNDI CATATG 1 cut(s) 1402
Fnu4HI GCNGC 2 cut(s) 1630, 1652
FokI GGATG 3 cut(s) 607, 689, 1190
FriOI GRGCYC 1 cut(s) 498
Fsp4HI GCNGC 2 cut(s) 1630, 1652
FspBI CTAG 3 cut(s) 1214, 1500, 1557
GlaI GCGC 2 cut(s) 320, 587
GluI GCNGC 2 cut(s) 1630, 1652
GsuI CTGGAG 1 cut(s) 1682
HaeII RGCGCY 1 cut(s) 322
HaeIII GGCC 4 cut(s) 219, 395, 722, 1551
HgaI GACGC 1 cut(s) 914
HhaI GCGC 2 cut(s) 321, 588
Hin1II CATG 8 cut(s) 260, 283, 400, 580, 880, 935, 1228, 1427
Hin6I GCGC 2 cut(s) 319, 586
HinP1I GCGC 2 cut(s) 319, 586
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HindIII AAGCTT 3 cut(s) 212, 383, 1145
HinfI GANTC 3 cut(s) 293, 701, 1121
HpaI GTTAAC 1 cut(s) 658
HphI GGTGA 2 cut(s) 229, 914
Hpy166II GTNNAC 3 cut(s) 488, 658, 1316
Hpy188I TCNGA 5 cut(s) 30, 714, 984, 989, 1335
Hpy188III TCNNGA 6 cut(s) 370, 503, 647, 854, 1033, 1175
Hpy8I GTNNAC 3 cut(s) 488, 658, 1316
HpyAV CCTTC 6 cut(s) 158, 367, 371, 585, 602, 1426
HpyF10VI GCNNNNNNNGC 5 cut(s) 182, 914, 1144, 1648, 1657
HpyF3I CTNAG 1 cut(s) 1533
Hsp92II CATG 8 cut(s) 260, 283, 400, 580, 880, 935, 1228, 1427
HspAI GCGC 2 cut(s) 319, 586
KspAI GTTAAC 1 cut(s) 658
Kzo9I GATC 4 cut(s) 73, 505, 649, 984
LguI GCTCTTC 2 cut(s) 133, 1108
LmnI GCTCC 1 cut(s) 1561
Lsp1109I GCAGC 1 cut(s) 1663
LweI GCATC 9 cut(s) 172, 277, 991, 1084, 1106, 1125, 1147, 1333, 1566
MaeI CTAG 3 cut(s) 1214, 1500, 1557
MaeIII GTNAC 3 cut(s) 680, 902, 974
MalI GATC 4 cut(s) 75, 507, 651, 986
MboI GATC 4 cut(s) 73, 505, 649, 984
MboII GAAGA 6 cut(s) 120, 342, 1092, 1125, 1381, 1596
MflI RGATCY 1 cut(s) 73
MhlI GDGCHC 1 cut(s) 498
MluCI AATT 9 cut(s) 204, 268, 462, 547, 615, 954, 1083, 1125, 1623
MlyI GAGTC 1 cut(s) 710
Mph1103I ATGCAT 3 cut(s) 889, 1099, 1140
MroXI GAANNNNTTC 1 cut(s) 205
MslI CAYNNNNRTG 1 cut(s) 1238
Mva1269I GAATGC 1 cut(s) 887
MvnI CGCG 1 cut(s) 654
MwoI GCNNNNNNNGC 5 cut(s) 182, 914, 1144, 1648, 1657
NdeI CATATG 1 cut(s) 1402
NdeII GATC 4 cut(s) 73, 505, 649, 984
NlaIII CATG 8 cut(s) 260, 283, 400, 580, 880, 935, 1228, 1427
NlaIV GGNNCC 2 cut(s) 75, 1591
NmuCI GTSAC 1 cut(s) 902
NsiI ATGCAT 3 cut(s) 889, 1099, 1140
NspV TTCGAA 1 cut(s) 199
OliI CACNNNNGTG 1 cut(s) 1238
PciSI GCTCTTC 2 cut(s) 133, 1108
PctI GAATGC 1 cut(s) 887
PdmI GAANNNNTTC 1 cut(s) 205
PfeI GAWTC 2 cut(s) 293, 1121
PkrI GCNGC 2 cut(s) 1631, 1653
PleI GAGTC 1 cut(s) 709
PpsI GAGTC 1 cut(s) 709
PpuMI RGGWCCY 1 cut(s) 1590
PshBI ATTAAT 2 cut(s) 614, 882
PsiI TTATAA 1 cut(s) 1185
Psp124BI GAGCTC 1 cut(s) 498
Psp5II RGGWCCY 1 cut(s) 1590
PspN4I GGNNCC 2 cut(s) 75, 1591
PspPI GGNCC 3 cut(s) 721, 1549, 1590
PspPPI RGGWCCY 1 cut(s) 1590
PstNI CAGNNNCTG 1 cut(s) 296
PsuI RGATCY 1 cut(s) 73
RsaI GTAC 3 cut(s) 300, 555, 826
RsaNI GTAC 3 cut(s) 299, 554, 825
RseI CAYNNNNRTG 1 cut(s) 1238
SacI GAGCTC 1 cut(s) 498
SapI GCTCTTC 2 cut(s) 133, 1108
SatI GCNGC 2 cut(s) 1630, 1652
Sau3AI GATC 4 cut(s) 73, 505, 649, 984
Sau96I GGNCC 3 cut(s) 721, 1549, 1590
SchI GAGTC 1 cut(s) 710
SduI GDGCHC 1 cut(s) 498
SfaNI GCATC 9 cut(s) 172, 277, 991, 1084, 1106, 1125, 1147, 1333, 1566
SfcI CTRYAG 1 cut(s) 892
SfuI TTCGAA 1 cut(s) 199
SinI GGWCC 1 cut(s) 1590
SmiMI CAYNNNNRTG 1 cut(s) 1238
Sse9I AATT 9 cut(s) 204, 268, 462, 547, 615, 954, 1083, 1125, 1623
SsiI CCGC 3 cut(s) 1346, 1538, 1629
SspI AATATT 1 cut(s) 337
SspMI CTAG 3 cut(s) 1214, 1500, 1557
SstI GAGCTC 1 cut(s) 498
TaqI TCGA 1 cut(s) 199
TasI AATT 9 cut(s) 204, 268, 462, 547, 615, 954, 1083, 1125, 1623
TatI WGTACW 3 cut(s) 298, 553, 824
TauI GCSGC 1 cut(s) 1632
TfiI GAWTC 2 cut(s) 293, 1121
TseFI GTSAC 1 cut(s) 902
TseI GCWGC 1 cut(s) 1651
Tsp45I GTSAC 1 cut(s) 902
TspGWI ACGGA 1 cut(s) 121
VpaK11BI GGWCC 1 cut(s) 1590
VspI ATTAAT 2 cut(s) 614, 882
XapI RAATTY 1 cut(s) 1083
XcmI CCANNNNNNNNNTGG 1 cut(s) 164
XmnI GAANNNNTTC 1 cut(s) 205
XspI CTAG 3 cut(s) 1214, 1500, 1557
Zsp2I ATGCAT 3 cut(s) 889, 1099, 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.