MD11G1161200.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0006142
Physical Location & Seq
Reverse (-)
15927973 .. 15928950
978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1161200.v1.1.491

Sequence Viewer

Length: 978 bp
ATGTTCTTCAGTAAGCTCAAACCCTTTCATATTGCTCCATCTTTGAGTTTCTCATCAGCCAAACTGCATTGTTCAATTCCCATGACCATTCAAAACCCACCAATGTCTTCTCCTTCCCATGTTCAAGGCTACAGTTCTGCCATCCCTCCATCCAAGACCCACCTCTATGCATCATTCTTCTGCACCCTTGTACACCTTTACTTGACATGTGGCAGATTTTCCAATGCTTCTGGTGCCTTCCGTTCTATGCGAAATCACGGCCTTGTTCCTGATTTGCCGCTTTGGAATCAACTTCTTTACCAATTCAATTCCTCAGGTTGGGTTTCTCAGGTATCTCTTCTTTACTCTGAGATGCTTTCTTGTGGGGTTATGCCCAATGTTTTCACCTGCAACATATTGATTCATTCTTTCTGCAAAGTGGGAAACTTGAGTTTTGCACTAGATTTCCTGAGAACTGGTGAAATTGATACTGTTTCCTATAATACTGTCATATGGGGTTTCTGTAAAAAAGGATTGGCTTATCAGGCTTTTGGGTTTTTGACTCAAATGGTGAAGAGGGATATACCAATCGATTCATATACTTGCAATACACTAGTTAAAGGGTTTTGTCAAATTGGGTTGGTAGAGTACGCAGCACGGGTTATGGATAATTTGGTGGATGGTGGAATTCCACAGGATGTTGTAGGTTTTAACACCTTGATTGCTGGACATTGCAAAGCTGGACAAGTTAGTCAGGCACTTGAATTGATGGACAGAATGGGTGGGGAGAGTCTGTCTCCTGATATTATTACTTATAATACTCTGATTCATGGGTTTTGCAGCACGGGTGATTTTGCAAGGGCCAAGAGTCTTATAGATATGATGTTGAGATCTCAGACAAATGAAGAATGCCCTCATGATGAAAGAGATGATGGCCAAAATCAGACTGAAGATAAGAATTTGAAACCAAACCTTATAACACACACCCACACTTATTAG

Protein Analysis

326

Amino Acids

36.11

Weight (kDa)

6.62

Isoelectric Point (pI)

33.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 91 - 139 3.6e-07 PPR repeat family
PPR_1 PF12854 122 - 151 1.4e-06 PPR repeat
TPR_24 PF23276 151 - 249 9e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 155 - 184 3e-06 PPR repeat
PPR_2 PF13041 156 - 204 1.8e-14 PPR repeat family
PPR_3 PF13812 213 - 270 5.6e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 222 - 253 4e-09 PPR repeat
PPR_2 PF13041 226 - 273 1.1e-16 PPR repeat family
PPR_1 PF12854 258 - 288 4.9e-10 PPR repeat
PPR_2 PF13041 260 - 291 5.6e-07 PPR repeat family
PPR PF01535 263 - 291 2e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 795, 956
AarI CACCTGC 1 cut(s) 395
AasI GACNNNNNNGTC 1 cut(s) 729
Acc36I ACCTGC 1 cut(s) 395
AccB1I GGYRCC 1 cut(s) 233
AciI CCGC 1 cut(s) 278
AcoI YGGCCR 1 cut(s) 913
AcsI RAATTY 2 cut(s) 666, 937
AcuI CTGAAG 1 cut(s) 948
AfaI GTAC 2 cut(s) 192, 629
AfiI CCNNNNNNNGG 1 cut(s) 318
AflIII ACRYGT 1 cut(s) 206
AgsI TTSAA 6 cut(s) 75, 92, 125, 307, 743, 943
AhlI ACTAGT 1 cut(s) 592
AluBI AGCT 2 cut(s) 16, 719
AluI AGCT 2 cut(s) 16, 719
Alw26I GTCTC 1 cut(s) 780
AoxI GGCC 3 cut(s) 259, 840, 913
ApeKI GCWGC 2 cut(s) 632, 819
ApoI RAATTY 2 cut(s) 666, 937
AspS9I GGNCC 1 cut(s) 840
AsuHPI GGTGA 4 cut(s) 376, 470, 562, 839
AxyI CCTNAGG 1 cut(s) 313
BalI TGGCCA 1 cut(s) 915
BanI GGYRCC 1 cut(s) 233
BbsI GAAGAC 1 cut(s) 99
BbvI GCAGC 2 cut(s) 644, 831
BccI CCATC 6 cut(s) 46, 149, 157, 653, 742, 905
BceAI ACGGC 1 cut(s) 274
BcoDI GTCTC 1 cut(s) 780
BcuI ACTAGT 1 cut(s) 592
BfaI CTAG 2 cut(s) 440, 593
BfmI CTRYAG 1 cut(s) 130
BfuAI ACCTGC 1 cut(s) 395
BglII AGATCT 1 cut(s) 869
BisI GCNGC 3 cut(s) 278, 633, 820
BlsI GCNGC 3 cut(s) 279, 634, 821
BmgT120I GGNCC 1 cut(s) 840
BmiI GGNNCC 1 cut(s) 235
BmsI GCATC 2 cut(s) 179, 342
BpiI GAAGAC 1 cut(s) 99
BplI GAGNNNNNCTC 2 cut(s) 760, 792
BpuEI CTTGAG 1 cut(s) 448
Bsa29I ATCGAT 1 cut(s) 570
BsaXI ACNNNNNCTCC 2 cut(s) 758, 788
Bsc4I CCNNNNNNNGG 1 cut(s) 318
Bse1I ACTGG 1 cut(s) 460
Bse21I CCTNAGG 1 cut(s) 313
Bse3DI GCAATG 1 cut(s) 709
BseCI ATCGAT 1 cut(s) 570
BseGI GGATG 4 cut(s) 141, 149, 664, 682
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMI GCAATG 1 cut(s) 709
BseMII CTCAG 5 cut(s) 327, 339, 341, 440, 887
BseNI ACTGG 1 cut(s) 460
BseXI GCAGC 2 cut(s) 644, 831
BsgI GTGCAG 1 cut(s) 166
BshFI GGCC 3 cut(s) 261, 842, 915
BshNI GGYRCC 1 cut(s) 233
BshVI ATCGAT 1 cut(s) 570
BslI CCNNNNNNNGG 1 cut(s) 318
BsmAI GTCTC 1 cut(s) 780
BsmI GAATGC 1 cut(s) 893
BsnI GGCC 3 cut(s) 261, 842, 915
Bsp1407I TGTACA 1 cut(s) 190
Bsp143I GATC 1 cut(s) 869
BspACI CCGC 1 cut(s) 278
BspANI GGCC 3 cut(s) 261, 842, 915
BspCNI CTCAG 5 cut(s) 326, 340, 340, 441, 886
BspDI ATCGAT 1 cut(s) 570
BspHI TCATGA 1 cut(s) 895
BspLI GGNNCC 1 cut(s) 235
BspMI ACCTGC 1 cut(s) 395
BspT107I GGYRCC 1 cut(s) 233
BsrDI GCAATG 1 cut(s) 709
BsrGI TGTACA 1 cut(s) 190
BsrI ACTGG 1 cut(s) 460
BssMI GATC 1 cut(s) 869
Bst4CI ACNGT 3 cut(s) 134, 472, 487
Bst6I CTCTTC 2 cut(s) 342, 548
BstAUI TGTACA 1 cut(s) 190
BstDEI CTNAG 5 cut(s) 313, 327, 348, 449, 873
BstF5I GGATG 4 cut(s) 141, 149, 664, 682
BstKTI GATC 1 cut(s) 872
BstMAI GTCTC 1 cut(s) 780
BstMBI GATC 1 cut(s) 869
BstMWI GCNNNNNNNGC 2 cut(s) 233, 524
BstNSI RCATGY 1 cut(s) 210
BstSFI CTRYAG 1 cut(s) 130
BstV1I GCAGC 2 cut(s) 644, 831
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 1 cut(s) 869
BstYI RGATCY 1 cut(s) 869
Bsu15I ATCGAT 1 cut(s) 570
Bsu36I CCTNAGG 1 cut(s) 313
BsuRI GGCC 3 cut(s) 261, 842, 915
BsuTUI ATCGAT 1 cut(s) 570
BtsCI GGATG 4 cut(s) 141, 149, 664, 682
BveI ACCTGC 1 cut(s) 395
CciI TCATGA 1 cut(s) 895
Cfr13I GGNCC 1 cut(s) 840
ClaI ATCGAT 1 cut(s) 570
Csp6I GTAC 2 cut(s) 191, 628
CviAII CATG 5 cut(s) 82, 119, 207, 809, 896
CviJI RGCY 9 cut(s) 16, 59, 129, 261, 518, 527, 719, 842, 915
CviKI_1 RGCY 9 cut(s) 16, 59, 129, 261, 518, 527, 719, 842, 915
CviQI GTAC 2 cut(s) 191, 628
DdeI CTNAG 5 cut(s) 313, 327, 348, 449, 873
DpnI GATC 1 cut(s) 871
DpnII GATC 1 cut(s) 869
DrdI GACNNNNNNGTC 1 cut(s) 729
DseDI GACNNNNNNGTC 1 cut(s) 729
EaeI YGGCCR 1 cut(s) 913
Eam1104I CTCTTC 2 cut(s) 342, 548
EarI CTCTTC 2 cut(s) 342, 548
Eco57I CTGAAG 1 cut(s) 948
Eco81I CCTNAGG 1 cut(s) 313
EcoRI GAATTC 1 cut(s) 666
EcoT22I ATGCAT 1 cut(s) 172
FaeI CATG 5 cut(s) 85, 122, 210, 812, 899
FatI CATG 5 cut(s) 81, 118, 206, 808, 895
FauNDI CATATG 1 cut(s) 491
Fnu4HI GCNGC 3 cut(s) 278, 633, 820
FokI GGATG 4 cut(s) 128, 136, 671, 689
Fsp4HI GCNGC 3 cut(s) 278, 633, 820
FspBI CTAG 2 cut(s) 440, 593
GluI GCNGC 3 cut(s) 278, 633, 820
HaeIII GGCC 3 cut(s) 261, 842, 915
Hin1II CATG 5 cut(s) 85, 122, 210, 812, 899
HinfI GANTC 7 cut(s) 286, 400, 541, 572, 769, 805, 847
HphI GGTGA 4 cut(s) 376, 470, 562, 839
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 4 cut(s) 349, 804, 876, 924
Hpy188III TCNNGA 4 cut(s) 269, 448, 779, 896
Hpy8I GTNNAC 1 cut(s) 193
HpyAV CCTTC 2 cut(s) 123, 247
HpyCH4III ACNGT 3 cut(s) 134, 472, 487
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 524
HpyF3I CTNAG 5 cut(s) 313, 327, 348, 449, 873
Hsp92II CATG 5 cut(s) 85, 122, 210, 812, 899
Kzo9I GATC 1 cut(s) 869
LmnI GCTCC 1 cut(s) 40
Lsp1109I GCAGC 2 cut(s) 644, 831
LweI GCATC 2 cut(s) 179, 342
MaeI CTAG 2 cut(s) 440, 593
MalI GATC 1 cut(s) 871
MboI GATC 1 cut(s) 869
MboII GAAGA 6 cut(s) 99, 169, 329, 565, 896, 941
MflI RGATCY 1 cut(s) 869
MlsI TGGCCA 1 cut(s) 915
MluCI AATT 9 cut(s) 75, 302, 307, 462, 612, 649, 666, 743, 937
MluNI TGGCCA 1 cut(s) 915
MlyI GAGTC 3 cut(s) 535, 778, 856
MnlI CCTC 5 cut(s) 156, 173, 322, 549, 903
Mox20I TGGCCA 1 cut(s) 915
Mph1103I ATGCAT 1 cut(s) 172
MscI TGGCCA 1 cut(s) 915
MseI TTAA 2 cut(s) 597, 690
MslI CAYNNNNRTG 1 cut(s) 165
Msp20I TGGCCA 1 cut(s) 915
Mva1269I GAATGC 1 cut(s) 893
MwoI GCNNNNNNNGC 2 cut(s) 233, 524
NdeI CATATG 1 cut(s) 491
NdeII GATC 1 cut(s) 869
NlaIII CATG 5 cut(s) 85, 122, 210, 812, 899
NlaIV GGNNCC 1 cut(s) 235
NsiI ATGCAT 1 cut(s) 172
NspI RCATGY 1 cut(s) 210
PagI TCATGA 1 cut(s) 895
PaqCI CACCTGC 1 cut(s) 395
PciI ACATGT 1 cut(s) 206
PctI GAATGC 1 cut(s) 893
PfeI GAWTC 4 cut(s) 286, 400, 572, 805
PkrI GCNGC 3 cut(s) 279, 634, 821
PleI GAGTC 3 cut(s) 535, 777, 855
PpsI GAGTC 3 cut(s) 535, 777, 855
PscI ACATGT 1 cut(s) 206
PsiI TTATAA 2 cut(s) 795, 956
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 1 cut(s) 840
PsuI RGATCY 1 cut(s) 869
RsaI GTAC 2 cut(s) 192, 629
RsaNI GTAC 2 cut(s) 191, 628
RseI CAYNNNNRTG 1 cut(s) 165
SaqAI TTAA 2 cut(s) 597, 690
SatI GCNGC 3 cut(s) 278, 633, 820
Sau3AI GATC 1 cut(s) 869
Sau96I GGNCC 1 cut(s) 840
SchI GAGTC 3 cut(s) 535, 778, 856
SfaNI GCATC 2 cut(s) 179, 342
SfcI CTRYAG 1 cut(s) 130
SmiMI CAYNNNNRTG 1 cut(s) 165
SmlI CTYRAG 1 cut(s) 427
SmoI CTYRAG 1 cut(s) 427
SpeI ACTAGT 1 cut(s) 592
Sse9I AATT 9 cut(s) 75, 302, 307, 462, 612, 649, 666, 743, 937
SsiI CCGC 1 cut(s) 278
SspMI CTAG 2 cut(s) 440, 593
TaaI ACNGT 3 cut(s) 134, 472, 487
TaqI TCGA 1 cut(s) 570
TasI AATT 9 cut(s) 75, 302, 307, 462, 612, 649, 666, 743, 937
TatI WGTACW 1 cut(s) 190
TauI GCSGC 1 cut(s) 280
TfiI GAWTC 4 cut(s) 286, 400, 572, 805
Tru1I TTAA 2 cut(s) 597, 690
Tru9I TTAA 2 cut(s) 597, 690
TseI GCWGC 2 cut(s) 632, 819
TspDTI ATGAA 6 cut(s) 17, 392, 564, 797, 897, 915
TspGWI ACGGA 1 cut(s) 230
XapI RAATTY 2 cut(s) 666, 937
XceI RCATGY 1 cut(s) 210
XspI CTAG 2 cut(s) 440, 593
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.