MD01G1088300.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0005503
Physical Location & Seq
Reverse (-)
19933628 .. 19935181
1554 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1088300.v1.1.491

Sequence Viewer

Length: 1251 bp
ATGGCGCCTGAAAACATGAGCATATCTGTAAATGGGCAATCTCAAGTCCCTCCTGGTTTCAGGTTTCATCCAACCGAAGAGGAACTCTTGCAGTACTATTTGAAGAAGAAGGTCTCAAATCAGAGGATTGATCTCGATGTCATACGTGAAGTCGATCTCAATAAGCTTGAGCCATGGGATATACAAGAGAAGTGTAAGATAGGAACTACACCACAGAATGATTGGTATTTCTTCAGCCATAAAGACAAGAAGTACCCGACTGGAACACGAACAAATCGTGCAACTGCTGTCGGATTCTGGAAGGCAACTGGCCGTGATAAAGTGATATGTAGCAACTGCAGGCGGATCGGTATGAGGAAGACTCTTGTGTTCTACAAGGGCCGAGCTCCCCATGGCCAAAAGTCTGATTGGATCATGCATGAATATAGACTTGACGACAATAATACTAGCAATTGCAATATCACTAATGTACCCACTGTTGTGGGAGATGCAGCACAAGAGGAGGGATGGGTGGTTTGCCGAATTTTCAAGAAGAAAAACCTCCACAAAATTTTGAGCAGCCCAATCTTGAGTACAACTACATCCATCACAACAGAAACAAGAAGTGGCCAATCACTGTTTGATTCGTGTCCCGAGGGAGCTTTGGAGCAAATACTTCAATACATGGGGAGGACATGCAAGGAAGAAGAGAATGAAGCCTACATTAACAATAATAGCACAAGATTTAATCTCCAACCAATCAACACAGGCATTAGTAATACTAGTAATCACAATGGCTTCCATGAGAAGTTCTTGAAACTTCCGACCCTAGACAGCCCAAACTCCACAAGCAGCCAGGATTGTTACCAACCAAACATTCATGAGGGGATAATGGTCACAGAGAATAATAACAACCCGGTGAGTCCTTTCACTGATCATCACCAAAATATGGATTACCATGCACACCACATGGACTCAGGAATCACCAACTGGGCAGCTCTCGACCGCCTTGTGGCTTCACAACTCAATGGACAAACCGAGACCTCTAGGCAGTTAGCTTGTTTCAATATCACTGACCCTTATGACAACGATGATCATGATGATGAACTCCAGTTACCAAGTACCCTACGAGGACCGTTGTCTTCATCCAACACCTACCATGCCACACACGACTACAACAACAGGGAGATCGACCTGTGGAATTTCTATCGACAATCATCACCGTTGTCATCCTCCGACACGCTGTGCCACGTGTCGAACGGTCCTATATAA

Protein Analysis

417

Amino Acids

47.41

Weight (kDa)

6.01

Isoelectric Point (pI)

51.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 17 - 144 7.8e-46 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 928
AciI CCGC 2 cut(s) 343, 985
AclWI GGATC 2 cut(s) 353, 419
AcoI YGGCCR 3 cut(s) 310, 394, 607
AcsI RAATTY 3 cut(s) 522, 549, 1180
AcuI CTGAAG 1 cut(s) 217
AcvI CACGTG 1 cut(s) 1231
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 1224
AfaI GTAC 5 cut(s) 95, 254, 471, 574, 1102
AfiI CCNNNNNNNGG 2 cut(s) 928, 991
AflIII ACRYGT 1 cut(s) 1230
AgsI TTSAA 5 cut(s) 103, 529, 659, 796, 1045
AhlI ACTAGT 1 cut(s) 761
AjnI CCWGG 2 cut(s) 52, 834
AjuI GAANNNNNNNTTGG 2 cut(s) 840, 872
AleI CACNNNNGTG 1 cut(s) 479
AluBI AGCT 5 cut(s) 166, 386, 641, 977, 1037
AluI AGCT 5 cut(s) 166, 386, 641, 977, 1037
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 2 cut(s) 118, 1013
AlwI GGATC 2 cut(s) 353, 419
Ama87I CYCGRG 1 cut(s) 632
AoxI GGCC 4 cut(s) 310, 379, 394, 607
ApeKI GCWGC 4 cut(s) 491, 558, 831, 974
ApoI RAATTY 3 cut(s) 522, 549, 1180
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 3 cut(s) 379, 1112, 1241
AsuC2I CCSGG 1 cut(s) 896
AsuHPI GGTGA 4 cut(s) 910, 911, 955, 1191
AvaI CYCGRG 1 cut(s) 632
AvaII GGWCC 2 cut(s) 1112, 1241
BaeI ACNNNNGTAYC 2 cut(s) 453, 486
BalI TGGCCA 2 cut(s) 396, 609
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 388
BbrPI CACGTG 1 cut(s) 1231
BbsI GAAGAC 2 cut(s) 365, 1113
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 4 cut(s) 503, 570, 843, 986
BccI CCATC 2 cut(s) 501, 593
BceAI ACGGC 1 cut(s) 297
BcgI CGANNNNNNTGC 2 cut(s) 328, 362
BciT130I CCWGG 2 cut(s) 54, 836
BclI TGATCA 2 cut(s) 913, 1072
BcnI CCSGG 1 cut(s) 896
BcoDI GTCTC 2 cut(s) 118, 1013
BcuI ACTAGT 1 cut(s) 761
BfaI CTAG 4 cut(s) 447, 762, 809, 1026
BfmI CTRYAG 1 cut(s) 337
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 4 cut(s) 492, 559, 832, 975
BlsI GCNGC 4 cut(s) 493, 560, 833, 976
BmcAI AGTACT 1 cut(s) 95
Bme1390I CCNGG 3 cut(s) 54, 836, 896
Bme18I GGWCC 2 cut(s) 1112, 1241
BmeT110I CYCGRG 1 cut(s) 632
BmgT120I GGNCC 3 cut(s) 379, 1112, 1241
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 3 cut(s) 54, 836, 896
BmrI ACTGGG 1 cut(s) 979
BmsI GCATC 1 cut(s) 478
BmuI ACTGGG 1 cut(s) 979
BoxI GACNNNNGTC 1 cut(s) 1117
BpiI GAAGAC 2 cut(s) 365, 1113
BplI GAGNNNNNCTC 2 cut(s) 346, 378
BpmI CTGGAG 1 cut(s) 1073
BpuEI CTTGAG 3 cut(s) 27, 188, 589
BpuMI CCSGG 1 cut(s) 896
BsaAI YACGTR 2 cut(s) 146, 1231
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 118, 1013
BsaJI CCNNGG 3 cut(s) 173, 391, 633
Bsc4I CCNNNNNNNGG 2 cut(s) 928, 991
Bse1I ACTGG 4 cut(s) 265, 313, 974, 1090
BseBI CCWGG 2 cut(s) 54, 836
BseDI CCNNGG 3 cut(s) 173, 391, 633
BseGI GGATG 5 cut(s) 67, 512, 581, 1124, 1208
BseLI CCNNNNNNNGG 2 cut(s) 928, 991
BseMII CTCAG 1 cut(s) 969
BseNI ACTGG 4 cut(s) 265, 313, 974, 1090
BseRI GAGGAG 1 cut(s) 515
BseXI GCAGC 4 cut(s) 503, 570, 843, 986
Bsh1285I CGRYCG 1 cut(s) 985
BshFI GGCC 4 cut(s) 312, 381, 396, 609
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 985
BsiHKAI GWGCWC 1 cut(s) 388
BsiHKCI CYCGRG 1 cut(s) 632
BsiSI CCGG 1 cut(s) 896
BslFI GGGAC 2 cut(s) 32, 615
BslI CCNNNNNNNGG 2 cut(s) 928, 991
BsmAI GTCTC 2 cut(s) 118, 1013
BsmFI GGGAC 2 cut(s) 32, 615
BsnI GGCC 4 cut(s) 312, 381, 396, 609
Bso31I GGTCTC 2 cut(s) 118, 1013
BsoBI CYCGRG 1 cut(s) 632
Bsp1286I GDGCHC 1 cut(s) 388
Bsp143I GATC 7 cut(s) 130, 154, 345, 411, 913, 1072, 1167
Bsp19I CCATGG 2 cut(s) 173, 391
BspACI CCGC 2 cut(s) 343, 985
BspANI GGCC 4 cut(s) 312, 381, 396, 609
BspCNI CTCAG 1 cut(s) 968
BspHI TCATGA 2 cut(s) 859, 1075
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 341
BspPI GGATC 2 cut(s) 353, 419
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 2 cut(s) 118, 1013
BsrI ACTGG 4 cut(s) 265, 313, 974, 1090
BssECI CCNNGG 3 cut(s) 173, 391, 633
BssMI GATC 7 cut(s) 130, 154, 345, 411, 913, 1072, 1167
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 173, 391
Bst2UI CCWGG 2 cut(s) 54, 836
Bst4CI ACNGT 5 cut(s) 478, 618, 1116, 1203, 1241
Bst6I CTCTTC 2 cut(s) 72, 681
BstACI GRCGYC 1 cut(s) 5
BstBAI YACGTR 2 cut(s) 146, 1231
BstC8I GCNNGC 1 cut(s) 341
BstDEI CTNAG 1 cut(s) 955
BstDSI CCRYGG 2 cut(s) 173, 391
BstF5I GGATG 5 cut(s) 67, 512, 581, 1124, 1208
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 7 cut(s) 133, 157, 348, 414, 916, 1075, 1170
BstMAI GTCTC 2 cut(s) 118, 1013
BstMBI GATC 7 cut(s) 130, 154, 345, 411, 913, 1072, 1167
BstMCI CGRYCG 1 cut(s) 985
BstNI CCWGG 2 cut(s) 54, 836
BstNSI RCATGY 1 cut(s) 678
BstPAI GACNNNNGTC 1 cut(s) 1117
BstSCI CCNGG 3 cut(s) 52, 834, 894
BstSFI CTRYAG 1 cut(s) 337
BstV1I GCAGC 4 cut(s) 503, 570, 843, 986
BstV2I GAAGAC 2 cut(s) 365, 1113
BstXI CCANNNNNNTGG 1 cut(s) 481
BsuRI GGCC 4 cut(s) 312, 381, 396, 609
BtgI CCRYGG 2 cut(s) 173, 391
BtsCI GGATG 5 cut(s) 67, 512, 581, 1124, 1208
BtsIMutI CAGTG 4 cut(s) 474, 614, 909, 1050
Cac8I GCNNGC 1 cut(s) 341
CciI TCATGA 2 cut(s) 859, 1075
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 3 cut(s) 379, 1112, 1241
Csp6I GTAC 5 cut(s) 94, 253, 470, 573, 1101
CviQI GTAC 5 cut(s) 94, 253, 470, 573, 1101
DdeI CTNAG 1 cut(s) 955
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 132, 156, 347, 413, 915, 1074, 1169
DpnII GATC 7 cut(s) 130, 154, 345, 411, 913, 1072, 1167
DraIII CACNNNGTG 1 cut(s) 1224
EaeI YGGCCR 3 cut(s) 310, 394, 607
Eam1104I CTCTTC 2 cut(s) 72, 681
EarI CTCTTC 2 cut(s) 72, 681
EciI GGCGGA 1 cut(s) 358
Ecl136II GAGCTC 1 cut(s) 386
Eco130I CCWWGG 2 cut(s) 173, 391
Eco24I GRGCYC 1 cut(s) 388
Eco31I GGTCTC 2 cut(s) 118, 1013
Eco47I GGWCC 2 cut(s) 1112, 1241
Eco53kI GAGCTC 1 cut(s) 386
Eco57I CTGAAG 1 cut(s) 217
Eco72I CACGTG 1 cut(s) 1231
Eco88I CYCGRG 1 cut(s) 632
EcoICRI GAGCTC 1 cut(s) 386
EcoRII CCWGG 2 cut(s) 52, 834
EcoT14I CCWWGG 2 cut(s) 173, 391
EcoT22I ATGCAT 1 cut(s) 420
EcoT38I GRGCYC 1 cut(s) 388
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 173, 391
FaqI GGGAC 2 cut(s) 32, 615
FbaI TGATCA 2 cut(s) 913, 1072
Fnu4HI GCNGC 4 cut(s) 492, 559, 832, 975
FokI GGATG 5 cut(s) 54, 519, 568, 1111, 1195
FriOI GRGCYC 1 cut(s) 388
Fsp4HI GCNGC 4 cut(s) 492, 559, 832, 975
FspBI CTAG 4 cut(s) 447, 762, 809, 1026
GlaI GCGC 1 cut(s) 6
GluI GCNGC 4 cut(s) 492, 559, 832, 975
GsuI CTGGAG 1 cut(s) 1073
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 4 cut(s) 312, 381, 396, 609
HapII CCGG 1 cut(s) 896
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HindIII AAGCTT 1 cut(s) 164
HinfI GANTC 6 cut(s) 294, 361, 623, 901, 953, 960
HpaII CCGG 1 cut(s) 896
HphI GGTGA 4 cut(s) 910, 911, 955, 1191
Hpy188I TCNGA 5 cut(s) 123, 293, 406, 804, 1216
HpyAV CCTTC 2 cut(s) 103, 295
HpyCH4III ACNGT 5 cut(s) 478, 618, 1116, 1203, 1241
HpyCH4IV ACGT 2 cut(s) 145, 1230
HpyCH4V TGCA 8 cut(s) 91, 281, 339, 418, 456, 491, 678, 941
HpyF3I CTNAG 1 cut(s) 955
HpySE526I ACGT 2 cut(s) 145, 1230
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 2 cut(s) 913, 1072
Kzo9I GATC 7 cut(s) 130, 154, 345, 411, 913, 1072, 1167
LmnI GCTCC 3 cut(s) 391, 638, 646
Lsp1109I GCAGC 4 cut(s) 503, 570, 843, 986
LweI GCATC 1 cut(s) 478
MaeI CTAG 4 cut(s) 447, 762, 809, 1026
MaeII ACGT 2 cut(s) 145, 1230
MaeIII GTNAC 3 cut(s) 842, 874, 1092
MalI GATC 7 cut(s) 132, 156, 347, 413, 915, 1074, 1169
MboI GATC 7 cut(s) 130, 154, 345, 411, 913, 1072, 1167
MboII GAAGA 9 cut(s) 89, 115, 118, 223, 370, 544, 695, 698, 1113
MfeI CAATTG 1 cut(s) 451
MhlI GDGCHC 1 cut(s) 388
MlsI TGGCCA 2 cut(s) 396, 609
MluCI AATT 4 cut(s) 451, 522, 549, 1180
MluNI TGGCCA 2 cut(s) 396, 609
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 3 cut(s) 355, 910, 947
MmeI TCCRAC 6 cut(s) 95, 271, 757, 827, 1152, 1239
Mox20I TGGCCA 2 cut(s) 396, 609
Mph1103I ATGCAT 1 cut(s) 420
MscI TGGCCA 2 cut(s) 396, 609
MseI TTAA 2 cut(s) 705, 726
MslI CAYNNNNRTG 2 cut(s) 479, 1080
Msp20I TGGCCA 2 cut(s) 396, 609
MspI CCGG 1 cut(s) 896
MspR9I CCNGG 3 cut(s) 54, 836, 896
MunI CAATTG 1 cut(s) 451
MvaI CCWGG 2 cut(s) 54, 836
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 896
NcoI CCATGG 2 cut(s) 173, 391
NdeII GATC 7 cut(s) 130, 154, 345, 411, 913, 1072, 1167
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 407
NmuCI GTSAC 1 cut(s) 874
NsiI ATGCAT 1 cut(s) 420
NspI RCATGY 1 cut(s) 678
OliI CACNNNNGTG 1 cut(s) 479
PagI TCATGA 2 cut(s) 859, 1075
PcsI WCGNNNNNNNCGW 1 cut(s) 274
PfeI GAWTC 3 cut(s) 294, 623, 960
PflMI CCANNNNNTGG 1 cut(s) 928
PkrI GCNGC 4 cut(s) 493, 560, 833, 976
PleI GAGTC 3 cut(s) 355, 909, 947
PluTI GGCGCC 1 cut(s) 8
PmaCI CACGTG 1 cut(s) 1231
PmlI CACGTG 1 cut(s) 1231
PpsI GAGTC 3 cut(s) 355, 909, 947
Ppu21I YACGTR 2 cut(s) 146, 1231
PshAI GACNNNNGTC 1 cut(s) 1117
Psp124BI GAGCTC 1 cut(s) 388
Psp6I CCWGG 2 cut(s) 52, 834
PspCI CACGTG 1 cut(s) 1231
PspGI CCWGG 2 cut(s) 52, 834
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 3 cut(s) 379, 1112, 1241
PsrI GAACNNNNNNTAC 2 cut(s) 1077, 1109
PstI CTGCAG 1 cut(s) 341
RsaI GTAC 5 cut(s) 95, 254, 471, 574, 1102
RsaNI GTAC 5 cut(s) 94, 253, 470, 573, 1101
RseI CAYNNNNRTG 2 cut(s) 479, 1080
SacI GAGCTC 1 cut(s) 388
SaqAI TTAA 2 cut(s) 705, 726
SatI GCNGC 4 cut(s) 492, 559, 832, 975
Sau3AI GATC 7 cut(s) 130, 154, 345, 411, 913, 1072, 1167
Sau96I GGNCC 3 cut(s) 379, 1112, 1241
ScaI AGTACT 1 cut(s) 95
SchI GAGTC 3 cut(s) 355, 910, 947
ScrFI CCNGG 3 cut(s) 54, 836, 896
SduI GDGCHC 1 cut(s) 388
SfaNI GCATC 1 cut(s) 478
SfcI CTRYAG 1 cut(s) 337
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 2 cut(s) 1112, 1241
SmiMI CAYNNNNRTG 2 cut(s) 479, 1080
SmlI CTYRAG 3 cut(s) 42, 167, 568
SmoI CTYRAG 3 cut(s) 42, 167, 568
SpeI ACTAGT 1 cut(s) 761
Sse9I AATT 4 cut(s) 451, 522, 549, 1180
SsiI CCGC 2 cut(s) 343, 985
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 4 cut(s) 447, 762, 809, 1026
SstI GAGCTC 1 cut(s) 388
StyD4I CCNGG 3 cut(s) 52, 834, 894
StyI CCWWGG 2 cut(s) 173, 391
TaaI ACNGT 5 cut(s) 478, 618, 1116, 1203, 1241
TaiI ACGT 2 cut(s) 148, 1233
TaqI TCGA 6 cut(s) 135, 153, 981, 1170, 1189, 1235
TasI AATT 4 cut(s) 451, 522, 549, 1180
TatI WGTACW 2 cut(s) 93, 572
TfiI GAWTC 3 cut(s) 294, 623, 960
Tru1I TTAA 2 cut(s) 705, 726
Tru9I TTAA 2 cut(s) 705, 726
TscAI CASTG 4 cut(s) 481, 621, 916, 1057
TseFI GTSAC 1 cut(s) 874
TseI GCWGC 4 cut(s) 491, 558, 831, 974
Tsp45I GTSAC 1 cut(s) 874
TspDTI ATGAA 6 cut(s) 56, 435, 708, 848, 1098, 1113
TspRI CASTG 4 cut(s) 481, 621, 916, 1057
Van91I CCANNNNNTGG 1 cut(s) 928
VpaK11BI GGWCC 2 cut(s) 1112, 1241
XapI RAATTY 3 cut(s) 522, 549, 1180
XceI RCATGY 1 cut(s) 678
XcmI CCANNNNNNNNNTGG 1 cut(s) 219
XspI CTAG 4 cut(s) 447, 762, 809, 1026
ZrmI AGTACT 1 cut(s) 95
Zsp2I ATGCAT 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.