MD08G1232800.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005503
Physical Location & Seq
Reverse (-)
29945218 .. 29950249
5032 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1232800.v1.1.491

Sequence Viewer

Length: 1155 bp
ATGAGATTTACAATCATAGGGTTTCATCCAACCGAAGAGGAACTCTTGCAGTACTATTTGAAGAAGAAGGTCTCAAATCAGAGGATCGATCTCGATGTCATACGTGAAGTCGATCTCAATAAGCTTGAGCCATGGGATATACAAGAGAAGTGTAAGATAGGAACTGCACCACAGAATGATTGGTATTTCTTCAGCCATAAAGACAAGAAGTACCCGACTGGAATACGAACAAATCGTGCAACTGCTGCTGGATTCTGGAAGGCAACTGGCCGTGATAAAGTGATATGTAGCAACTGCAGGCGGATCGGTATGAGGAAGACTCTTGTGTTCTACAAAGGCCGAGCTCCCCATGGCCAAAAGTCTGATTGGATCATGCATGAATATAGACTCGACGACAATAATACTAGCAATTGCAATATCACTAATGTACCCACTGTTGTGGGAGATGCAGCACAAGAGGAGGGATGGGTGGTTTGCCGAGTTTTCAAGAAGAAAAACCTCCACAAAATTTTGAGCAGCCCAATCTTGAATACTACTACATCCATCACAACAGAAACAAGAAATGGCCAATCACTGTTTGATTCGTGTCCCGAGGGAGCTTTGGAGCCGATACTTCATTACATGGGGAGGACATGCAATGAAGAAGGGAATGAAGCCTACATTAACAATAACAGCACAAGATTTAATCTCCAACCAAACAACACAGGCATTAGTAATACTAGTAATCACAATGGCTTCCATGAGAAGTTCTTGAAACTTCCGACCCTAGACAGCCCAAACTCCACAAGCAGCCAGGATTGTTACCAACCAAACATTCATGAGGGGATGATGGTCACAGAGAATAATAACAACCCGGTGAGTCCTTTTACTGATCATCACCAAAATATGGATTACCATGCACACCACATGGACTCAGGAATCACCAACTGGGCAGCTCTTGACCGCCTTGTGGCTTCACAACTCAATGGACAAACTGAGACCTCTAGGCAGTTAACTTGTTTCAATATCACTGACCCTTATGACAACGATGATCATGATCATGAACGCCAGTTACCAAGTACCCTACGAGGATCGTTGTCTTCATCCAACACCTACCATGCCACACACGACTACAACAACAGCGAGATCGACCTGTGGAATTTCGATCGACAATAA

Protein Analysis

385

Amino Acids

44.09

Weight (kDa)

6.21

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 7 - 130 1.5e-42 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 886
AciI CCGC 2 cut(s) 301, 943
AclWI GGATC 4 cut(s) 92, 311, 377, 1078
AcoI YGGCCR 3 cut(s) 268, 352, 565
AcsI RAATTY 2 cut(s) 507, 1138
AcuI CTGAAG 1 cut(s) 175
AfaI GTAC 4 cut(s) 53, 212, 429, 1060
AfiI CCNNNNNNNGG 2 cut(s) 886, 949
AgsI TTSAA 5 cut(s) 61, 487, 529, 754, 1003
AhlI ACTAGT 1 cut(s) 719
AjnI CCWGG 1 cut(s) 792
AjuI GAANNNNNNNTTGG 2 cut(s) 798, 830
AleI CACNNNNGTG 1 cut(s) 437
AluBI AGCT 4 cut(s) 124, 344, 599, 935
AluI AGCT 4 cut(s) 124, 344, 599, 935
Alw21I GWGCWC 1 cut(s) 346
Alw26I GTCTC 2 cut(s) 76, 971
AlwI GGATC 4 cut(s) 92, 311, 377, 1078
Ama87I CYCGRG 1 cut(s) 590
AoxI GGCC 4 cut(s) 268, 337, 352, 565
ApeKI GCWGC 5 cut(s) 245, 449, 516, 789, 932
ApoI RAATTY 2 cut(s) 507, 1138
AsuC2I CCSGG 1 cut(s) 854
AsuHPI GGTGA 3 cut(s) 868, 869, 913
AvaI CYCGRG 1 cut(s) 590
BaeI ACNNNNGTAYC 2 cut(s) 411, 444
BalI TGGCCA 2 cut(s) 354, 567
BanII GRGCYC 1 cut(s) 346
BbsI GAAGAC 2 cut(s) 323, 1071
Bbv12I GWGCWC 1 cut(s) 346
BbvI GCAGC 5 cut(s) 232, 461, 528, 801, 944
BccI CCATC 3 cut(s) 459, 551, 823
BceAI ACGGC 1 cut(s) 255
BcgI CGANNNNNNTGC 2 cut(s) 286, 320
BciT130I CCWGG 1 cut(s) 794
BclI TGATCA 3 cut(s) 871, 1030, 1036
BcnI CCSGG 1 cut(s) 854
BcoDI GTCTC 2 cut(s) 76, 971
BcuI ACTAGT 1 cut(s) 719
BfaI CTAG 4 cut(s) 405, 720, 767, 984
BfmI CTRYAG 1 cut(s) 295
BisI GCNGC 5 cut(s) 246, 450, 517, 790, 933
BlsI GCNGC 5 cut(s) 247, 451, 518, 791, 934
BmcAI AGTACT 1 cut(s) 53
Bme1390I CCNGG 2 cut(s) 794, 854
BmeT110I CYCGRG 1 cut(s) 590
BmiI GGNNCC 1 cut(s) 606
BmrFI CCNGG 2 cut(s) 794, 854
BmrI ACTGGG 1 cut(s) 937
BmsI GCATC 1 cut(s) 436
BmuI ACTGGG 1 cut(s) 937
BpiI GAAGAC 2 cut(s) 323, 1071
BplI GAGNNNNNCTC 2 cut(s) 304, 336
BpuEI CTTGAG 1 cut(s) 146
BpuMI CCSGG 1 cut(s) 854
Bsa29I ATCGAT 1 cut(s) 87
BsaAI YACGTR 1 cut(s) 104
BsaBI GATNNNNATC 1 cut(s) 1035
BsaI GGTCTC 2 cut(s) 76, 971
BsaJI CCNNGG 3 cut(s) 131, 349, 591
Bsc4I CCNNNNNNNGG 2 cut(s) 886, 949
Bse1I ACTGG 4 cut(s) 223, 271, 932, 1048
Bse3DI GCAATG 1 cut(s) 643
Bse8I GATNNNNATC 1 cut(s) 1035
BseBI CCWGG 1 cut(s) 794
BseCI ATCGAT 1 cut(s) 87
BseDI CCNNGG 3 cut(s) 131, 349, 591
BseGI GGATG 5 cut(s) 25, 470, 539, 831, 1082
BseJI GATNNNNATC 1 cut(s) 1035
BseLI CCNNNNNNNGG 2 cut(s) 886, 949
BseMI GCAATG 1 cut(s) 643
BseMII CTCAG 2 cut(s) 927, 966
BseNI ACTGG 4 cut(s) 223, 271, 932, 1048
BseRI GAGGAG 1 cut(s) 473
BseXI GCAGC 5 cut(s) 232, 461, 528, 801, 944
BsgI GTGCAG 1 cut(s) 150
Bsh1285I CGRYCG 1 cut(s) 1147
BshFI GGCC 4 cut(s) 270, 339, 354, 567
BshVI ATCGAT 1 cut(s) 87
BsiEI CGRYCG 1 cut(s) 1147
BsiHKAI GWGCWC 1 cut(s) 346
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 1 cut(s) 854
BslFI GGGAC 1 cut(s) 573
BslI CCNNNNNNNGG 2 cut(s) 886, 949
BsmAI GTCTC 2 cut(s) 76, 971
BsmFI GGGAC 1 cut(s) 573
BsnI GGCC 4 cut(s) 270, 339, 354, 567
Bso31I GGTCTC 2 cut(s) 76, 971
BsoBI CYCGRG 1 cut(s) 590
Bsp1286I GDGCHC 1 cut(s) 346
Bsp19I CCATGG 2 cut(s) 131, 349
BspACI CCGC 2 cut(s) 301, 943
BspANI GGCC 4 cut(s) 270, 339, 354, 567
BspCNI CTCAG 2 cut(s) 926, 967
BspDI ATCGAT 1 cut(s) 87
BspHI TCATGA 3 cut(s) 817, 1033, 1039
BspLI GGNNCC 1 cut(s) 606
BspMAI CTGCAG 1 cut(s) 299
BspPI GGATC 4 cut(s) 92, 311, 377, 1078
BspTNI GGTCTC 2 cut(s) 76, 971
BsrDI GCAATG 1 cut(s) 643
BsrI ACTGG 4 cut(s) 223, 271, 932, 1048
BssECI CCNNGG 3 cut(s) 131, 349, 591
BssT1I CCWWGG 2 cut(s) 131, 349
Bst2UI CCWGG 1 cut(s) 794
Bst4CI ACNGT 2 cut(s) 436, 576
Bst6I CTCTTC 1 cut(s) 30
BstAPI GCANNNNNTGC 1 cut(s) 245
BstBAI YACGTR 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 299
BstDEI CTNAG 2 cut(s) 913, 975
BstDSI CCRYGG 2 cut(s) 131, 349
BstF5I GGATG 5 cut(s) 25, 470, 539, 831, 1082
BstMAI GTCTC 2 cut(s) 76, 971
BstMCI CGRYCG 1 cut(s) 1147
BstMWI GCNNNNNNNGC 1 cut(s) 245
BstNI CCWGG 1 cut(s) 794
BstNSI RCATGY 1 cut(s) 636
BstSCI CCNGG 2 cut(s) 792, 852
BstSFI CTRYAG 1 cut(s) 295
BstV1I GCAGC 5 cut(s) 232, 461, 528, 801, 944
BstV2I GAAGAC 2 cut(s) 323, 1071
BstXI CCANNNNNNTGG 1 cut(s) 439
Bsu15I ATCGAT 1 cut(s) 87
BsuRI GGCC 4 cut(s) 270, 339, 354, 567
BsuTUI ATCGAT 1 cut(s) 87
BtgI CCRYGG 2 cut(s) 131, 349
BtsCI GGATG 5 cut(s) 25, 470, 539, 831, 1082
BtsIMutI CAGTG 3 cut(s) 432, 572, 1008
Cac8I GCNNGC 1 cut(s) 299
CciI TCATGA 3 cut(s) 817, 1033, 1039
ClaI ATCGAT 1 cut(s) 87
Csp6I GTAC 4 cut(s) 52, 211, 428, 1059
CviQI GTAC 4 cut(s) 52, 211, 428, 1059
DdeI CTNAG 2 cut(s) 913, 975
EaeI YGGCCR 3 cut(s) 268, 352, 565
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
EciI GGCGGA 1 cut(s) 316
Ecl136II GAGCTC 1 cut(s) 344
Eco130I CCWWGG 2 cut(s) 131, 349
Eco24I GRGCYC 1 cut(s) 346
Eco31I GGTCTC 2 cut(s) 76, 971
Eco53kI GAGCTC 1 cut(s) 344
Eco57I CTGAAG 1 cut(s) 175
Eco88I CYCGRG 1 cut(s) 590
EcoICRI GAGCTC 1 cut(s) 344
EcoRII CCWGG 1 cut(s) 792
EcoT14I CCWWGG 2 cut(s) 131, 349
EcoT22I ATGCAT 1 cut(s) 378
EcoT38I GRGCYC 1 cut(s) 346
ErhI CCWWGG 2 cut(s) 131, 349
FaqI GGGAC 1 cut(s) 573
FbaI TGATCA 3 cut(s) 871, 1030, 1036
Fnu4HI GCNGC 5 cut(s) 246, 450, 517, 790, 933
FokI GGATG 5 cut(s) 12, 477, 526, 838, 1069
FriOI GRGCYC 1 cut(s) 346
Fsp4HI GCNGC 5 cut(s) 246, 450, 517, 790, 933
FspBI CTAG 4 cut(s) 405, 720, 767, 984
GluI GCNGC 5 cut(s) 246, 450, 517, 790, 933
HaeIII GGCC 4 cut(s) 270, 339, 354, 567
HapII CCGG 1 cut(s) 854
HincII GTYRAC 1 cut(s) 993
HindII GTYRAC 1 cut(s) 993
HindIII AAGCTT 1 cut(s) 122
HinfI GANTC 7 cut(s) 252, 319, 387, 581, 859, 911, 918
HpaI GTTAAC 1 cut(s) 993
HpaII CCGG 1 cut(s) 854
HphI GGTGA 3 cut(s) 868, 869, 913
Hpy166II GTNNAC 1 cut(s) 993
Hpy188I TCNGA 3 cut(s) 81, 364, 762
Hpy8I GTNNAC 1 cut(s) 993
Hpy99I CGWCG 1 cut(s) 395
HpyAV CCTTC 3 cut(s) 61, 253, 638
HpyCH4III ACNGT 2 cut(s) 436, 576
HpyCH4IV ACGT 1 cut(s) 103
HpyCH4V TGCA 9 cut(s) 49, 167, 239, 297, 376, 414, 449, 636, 899
HpyF10VI GCNNNNNNNGC 1 cut(s) 245
HpyF3I CTNAG 2 cut(s) 913, 975
HpySE526I ACGT 1 cut(s) 103
Ksp22I TGATCA 3 cut(s) 871, 1030, 1036
KspAI GTTAAC 1 cut(s) 993
LmnI GCTCC 3 cut(s) 349, 596, 604
Lsp1109I GCAGC 5 cut(s) 232, 461, 528, 801, 944
LweI GCATC 1 cut(s) 436
MaeI CTAG 4 cut(s) 405, 720, 767, 984
MaeII ACGT 1 cut(s) 103
MaeIII GTNAC 3 cut(s) 800, 832, 1050
MboII GAAGA 8 cut(s) 47, 73, 76, 181, 328, 502, 653, 1071
MfeI CAATTG 1 cut(s) 409
MhlI GDGCHC 1 cut(s) 346
MlsI TGGCCA 2 cut(s) 354, 567
MluCI AATT 3 cut(s) 409, 507, 1138
MluNI TGGCCA 2 cut(s) 354, 567
MlyI GAGTC 4 cut(s) 313, 381, 868, 905
MmeI TCCRAC 4 cut(s) 53, 715, 785, 1110
Mox20I TGGCCA 2 cut(s) 354, 567
Mph1103I ATGCAT 1 cut(s) 378
MscI TGGCCA 2 cut(s) 354, 567
MseI TTAA 3 cut(s) 663, 684, 992
MslI CAYNNNNRTG 2 cut(s) 437, 1038
Msp20I TGGCCA 2 cut(s) 354, 567
MspI CCGG 1 cut(s) 854
MspR9I CCNGG 2 cut(s) 794, 854
MunI CAATTG 1 cut(s) 409
MvaI CCWGG 1 cut(s) 794
MwoI GCNNNNNNNGC 1 cut(s) 245
NciI CCSGG 1 cut(s) 854
NcoI CCATGG 2 cut(s) 131, 349
NlaIV GGNNCC 1 cut(s) 606
NmeAIII GCCGAG 2 cut(s) 365, 503
NmuCI GTSAC 1 cut(s) 832
NsiI ATGCAT 1 cut(s) 378
NspI RCATGY 1 cut(s) 636
OliI CACNNNNGTG 1 cut(s) 437
PagI TCATGA 3 cut(s) 817, 1033, 1039
PcsI WCGNNNNNNNCGW 1 cut(s) 232
PfeI GAWTC 3 cut(s) 252, 581, 918
PflMI CCANNNNNTGG 1 cut(s) 886
PkrI GCNGC 5 cut(s) 247, 451, 518, 791, 934
Ple19I CGATCG 1 cut(s) 1147
PleI GAGTC 4 cut(s) 313, 381, 867, 905
PpsI GAGTC 4 cut(s) 313, 381, 867, 905
Ppu21I YACGTR 1 cut(s) 104
Psp124BI GAGCTC 1 cut(s) 346
Psp6I CCWGG 1 cut(s) 792
PspGI CCWGG 1 cut(s) 792
PspN4I GGNNCC 1 cut(s) 606
PsrI GAACNNNNNNTAC 2 cut(s) 1035, 1067
PstI CTGCAG 1 cut(s) 299
PvuI CGATCG 1 cut(s) 1147
RsaI GTAC 4 cut(s) 53, 212, 429, 1060
RsaNI GTAC 4 cut(s) 52, 211, 428, 1059
RseI CAYNNNNRTG 2 cut(s) 437, 1038
SacI GAGCTC 1 cut(s) 346
SaqAI TTAA 3 cut(s) 663, 684, 992
SatI GCNGC 5 cut(s) 246, 450, 517, 790, 933
ScaI AGTACT 1 cut(s) 53
SchI GAGTC 4 cut(s) 313, 381, 868, 905
ScrFI CCNGG 2 cut(s) 794, 854
SduI GDGCHC 1 cut(s) 346
SfaNI GCATC 1 cut(s) 436
SfcI CTRYAG 1 cut(s) 295
SmiMI CAYNNNNRTG 2 cut(s) 437, 1038
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
SpeI ACTAGT 1 cut(s) 719
Sse9I AATT 3 cut(s) 409, 507, 1138
SsiI CCGC 2 cut(s) 301, 943
SspMI CTAG 4 cut(s) 405, 720, 767, 984
SstI GAGCTC 1 cut(s) 346
StyD4I CCNGG 2 cut(s) 792, 852
StyI CCWWGG 2 cut(s) 131, 349
TaaI ACNGT 2 cut(s) 436, 576
TaiI ACGT 1 cut(s) 106
TaqI TCGA 7 cut(s) 87, 93, 111, 390, 1128, 1143, 1147
TasI AATT 3 cut(s) 409, 507, 1138
TatI WGTACW 1 cut(s) 51
TfiI GAWTC 3 cut(s) 252, 581, 918
Tru1I TTAA 3 cut(s) 663, 684, 992
Tru9I TTAA 3 cut(s) 663, 684, 992
TscAI CASTG 3 cut(s) 439, 579, 1015
TseFI GTSAC 1 cut(s) 832
TseI GCWGC 5 cut(s) 245, 449, 516, 789, 932
Tsp45I GTSAC 1 cut(s) 832
TspDTI ATGAA 8 cut(s) 14, 393, 605, 654, 666, 806, 1056, 1071
TspRI CASTG 3 cut(s) 439, 579, 1015
Van91I CCANNNNNTGG 1 cut(s) 886
XapI RAATTY 2 cut(s) 507, 1138
XceI RCATGY 1 cut(s) 636
XcmI CCANNNNNNNNNTGG 1 cut(s) 177
XspI CTAG 4 cut(s) 405, 720, 767, 984
ZrmI AGTACT 1 cut(s) 53
Zsp2I ATGCAT 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.